Nepl14 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nepl14
Entrez Gene ID	42700
Full Name	CG4723 gene product from transcript CG4723-RA
Synonyms	Dmel\CG4723
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 94C4-94C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_142840.4	NP_651097.1	Neprilysin-like 14

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG4723	NP_651097.1
Drosophila melanogaster (fruit fly)	CG4725	NP_651096.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nepl14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nepl14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08698	NM_142840.4 Latest version!	Drosophila melanogaster Neprilysin-like 14 (Nepl14), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nepl14 ( NM_142840.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651097.1 Drosophila melanogaster Neprilysin-like 14 (Nepl14), mRNA.

Price & Availability↑

CloneID	OFa08698
Clone ID Related Accession (Same CDS sequence)	NM_142840.4
Accession Version	NM_142840.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Neprilysin-like 14
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_142840.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGACC CGTGTTGGAC GACAGTATTT TTATGGCTGC TCTGGCTGCC ACACCTCCAG 
CAAAATGCTT TGGTTTCTGG CGAGGATCTA CCACCGCCGT TCCAGCGGAT CTACCAATCG 
GAACAGACGG TACGACGTTC AAAGGCTCAG GATATGGGCA GTCATGTGAA CAAAAGCATC 
GATCCCTGTG TGGATTTCTA TGCATATGCA TGCGGCAATT GGAAGTCAAC TCTAACCCCA 
CAGCAGCAAT TGCAGCAGCA AACAGACCGG GAGCTTCTTC TGCTCGTGGA GGAGGCTGTA 
AGGAGAGAGG ATAGCGTCAT AGTTCGACAG GCCAAGGAGC TATTCAAGTC CTGCGTATCG 
GCACAGGCTC ATCAGAGCCA GCAGCAGCAG CATTTCCTCA GCGAGTTCAT CAACCAGAAC 
GGAGGCTTTC CAGCTGTTCC CGGTTCCCAA TGGGCAGTGC ATCACCATGA CTACAATTGG 
CTTAGTGTGT TAGGTGGTCT AAGACTTCGC TATGGAATGG ACATCCTGAT CGGACTCCGA 
GTGGGCTATA ACTATGGCAA TGTAAATGAG AACAGCTTGT ATTTAAGTGA ACCGAGCACA 
TTGATTCCAA CCGAGTTGTG CACTCGGAAT CGCTCAGATA TTCGGGACTC CATTTACGAA 
CCCCTTGAAC ATCGAGTGGC ATCCGAACTA AAATCTTGGT TGGCATTGGG ACACGATGAA 
TCCGCTCGTC TAGCTGCCGA CATTCTTAGT TTCGAGCATG AGTTATGTGG TGGAATGAAA 
GGTGGATCCT TTGAATCCTG GGATGCCGAG GAACAGCTCT ATCTAGCCAA CTACACCCGC 
AAAACGTTGC GTGAGTTCTC GAATTCGATC GAACTGGATT TGGATTCATA TGTAAGGGCA 
AGTTTTGGCC AGGATATTTA TAAACCCGTT TACATGGCAG CACCGGAATA TTTTCGCCAA 
CTTAAGCGCA CTGTGAGTGC CCACAACATA AGTCTAGTGG CTAACTATAT AATGTATCGA 
GCGGTGTCTG CTCTGAATTT TCCTCTAGAT GATCGCCCGC AGTTAAGGAA ACGGGAGTGC 
TTGGAACGAA CTAAAGAACT ACTTCCCACG GCATTGGGTG AATTATATGC CCGGCAGTTT 
GGAACTGAAG ATGGCAGAAT AGATTTGGAC AACCTTTATG ATACATTAAA GACAGCTTTG 
AGGCAAAGTC TAGGTGCGGA ATGGTTGGTG GAGGGCACTC AGCGGGTGGG CCAGGAAAAA 
CTTAGGAACC TAAAGCTGCA GATGCCCACT TACGAGCGTC CAGTGATCCT TAAACTGCAG 
CTGGAACGTG GCAATTATTG GCAAAATCTC CGCCAGCTGC TGGGAGTGGT TCAGTCCCAA 
AGAAACAATC GACTTTTCGA AGAGTTTTTG CCTGCTCCCA CCGATCCCGT GGAGGCCTAT 
GAGGCCAGGG TGCGACTGAG ACCAGTTCAG CGACGTTTGG ATATGGGTTT GGCCCTGCTG 
CAGCCACCAG CCTACGATCC TCGCTATGGC CATGCCATCC GCTATGCCAC ATTGGGCGTA 
CAGCTGGCGC AACAGTTGGT GATGGCTTTT GACGATCCGC ATTGGTCGGT GGGATTGCTC 
GAGCGGGATA ATTGGGATGG TCAAACAGCC TGGCAATATC ACATTCGAAG CAATTGCTTT 
GCCCGCCAGG TGGACAATTA TTTGAGGCCC AATGTAAGTG CCACCACGCA GCTGATCACG 
GACAGCGCAG CCATCAATGT CGCCTTTCGG GCGTATCTCA CATGGCTGGG TTTCCAGGAG 
CCAAACAACG ACTTCACTAA ACTGACCAAG GAGACTCTGC CGGATCTGAA CTACTCAAAC 
ACGGCACTAT TCTTCTTAGC TTATGCCCAA GAGCATTGCA GTCTGGAGGA GGCTCGTCCC 
AGGGATGAGC AAGGGACTCC GGAAGGATAC CTCTATAGTG CACGACAGAA TCACACCTGG 
ACTCGCCTGC TTGTAAACGG ACCTTTGCGA AACTCAGTTG AATTTGCGAG TGAATTTCGG 
TGCCCCATTG GATCACCCAT GAATCCTGCA TCCAAGTGCA TCATATACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651097.1
CDS	21..2111
Translation	MSDPCWTTVFLWLLWLPHLQQNALVSGEDLPPPFQRIYQSEQTVRRSKAQDMGSHVNKSIDPCVDFYAYACGNWKSTLTPQQQLQQQTDRELLLLVEEAVRREDSVIVRQAKELFKSCVSAQAHQSQQQQHFLSEFINQNGGFPAVPGSQWAVHHHDYNWLSVLGGLRLRYGMDILIGLRVGYNYGNVNENSLYLSEPSTLIPTELCTRNRSDIRDSIYEPLEHRVASELKSWLALGHDESARLAADILSFEHELCGGMKGGSFESWDAEEQLYLANYTRKTLREFSNSIELDLDSYVRASFGQDIYKPVYMAAPEYFRQLKRTVSAHNISLVANYIMYRAVSALNFPLDDRPQLRKRECLERTKELLPTALGELYARQFGTEDGRIDLDNLYDTLKTALRQSLGAEWLVEGTQRVGQEKLRNLKLQMPTYERPVILKLQLERGNYWQNLRQLLGVVQSQRNNRLFEEFLPAPTDPVEAYEARVRLRPVQRRLDMGLALLQPPAYDPRYGHAIRYATLGVQLAQQLVMAFDDPHWSVGLLERDNWDGQTAWQYHIRSNCFARQVDNYLRPNVSATTQLITDSAAINVAFRAYLTWLGFQEPNNDFTKLTKETLPDLNYSNTALFFLAYAQEHCSLEEARPRDEQGTPEGYLYSARQNHTWTRLLVNGPLRNSVEFASEFRCPIGSPMNPASKCIIY

Target ORF information:

RefSeq Version	NM_142840.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Neprilysin-like 14 (Nepl14), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142840.4

ORF Insert Sequence:

ATGTCGGACC CGTGTTGGAC GACAGTATTT TTATGGCTGC TCTGGCTGCC ACACCTCCAG 
CAAAATGCTT TGGTTTCTGG CGAGGATCTA CCACCGCCGT TCCAGCGGAT CTACCAATCG 
GAACAGACGG TACGACGTTC AAAGGCTCAG GATATGGGCA GTCATGTGAA CAAAAGCATC 
GATCCCTGTG TGGATTTCTA TGCATATGCA TGCGGCAATT GGAAGTCAAC TCTAACCCCA 
CAGCAGCAAT TGCAGCAGCA AACAGACCGG GAGCTTCTTC TGCTCGTGGA GGAGGCTGTA 
AGGAGAGAGG ATAGCGTCAT AGTTCGACAG GCCAAGGAGC TATTCAAGTC CTGCGTATCG 
GCACAGGCTC ATCAGAGCCA GCAGCAGCAG CATTTCCTCA GCGAGTTCAT CAACCAGAAC 
GGAGGCTTTC CAGCTGTTCC CGGTTCCCAA TGGGCAGTGC ATCACCATGA CTACAATTGG 
CTTAGTGTGT TAGGTGGTCT AAGACTTCGC TATGGAATGG ACATCCTGAT CGGACTCCGA 
GTGGGCTATA ACTATGGCAA TGTAAATGAG AACAGCTTGT ATTTAAGTGA ACCGAGCACA 
TTGATTCCAA CCGAGTTGTG CACTCGGAAT CGCTCAGATA TTCGGGACTC CATTTACGAA 
CCCCTTGAAC ATCGAGTGGC ATCCGAACTA AAATCTTGGT TGGCATTGGG ACACGATGAA 
TCCGCTCGTC TAGCTGCCGA CATTCTTAGT TTCGAGCATG AGTTATGTGG TGGAATGAAA 
GGTGGATCCT TTGAATCCTG GGATGCCGAG GAACAGCTCT ATCTAGCCAA CTACACCCGC 
AAAACGTTGC GTGAGTTCTC GAATTCGATC GAACTGGATT TGGATTCATA TGTAAGGGCA 
AGTTTTGGCC AGGATATTTA TAAACCCGTT TACATGGCAG CACCGGAATA TTTTCGCCAA 
CTTAAGCGCA CTGTGAGTGC CCACAACATA AGTCTAGTGG CTAACTATAT AATGTATCGA 
GCGGTGTCTG CTCTGAATTT TCCTCTAGAT GATCGCCCGC AGTTAAGGAA ACGGGAGTGC 
TTGGAACGAA CTAAAGAACT ACTTCCCACG GCATTGGGTG AATTATATGC CCGGCAGTTT 
GGAACTGAAG ATGGCAGAAT AGATTTGGAC AACCTTTATG ATACATTAAA GACAGCTTTG 
AGGCAAAGTC TAGGTGCGGA ATGGTTGGTG GAGGGCACTC AGCGGGTGGG CCAGGAAAAA 
CTTAGGAACC TAAAGCTGCA GATGCCCACT TACGAGCGTC CAGTGATCCT TAAACTGCAG 
CTGGAACGTG GCAATTATTG GCAAAATCTC CGCCAGCTGC TGGGAGTGGT TCAGTCCCAA 
AGAAACAATC GACTTTTCGA AGAGTTTTTG CCTGCTCCCA CCGATCCCGT GGAGGCCTAT 
GAGGCCAGGG TGCGACTGAG ACCAGTTCAG CGACGTTTGG ATATGGGTTT GGCCCTGCTG 
CAGCCACCAG CCTACGATCC TCGCTATGGC CATGCCATCC GCTATGCCAC ATTGGGCGTA 
CAGCTGGCGC AACAGTTGGT GATGGCTTTT GACGATCCGC ATTGGTCGGT GGGATTGCTC 
GAGCGGGATA ATTGGGATGG TCAAACAGCC TGGCAATATC ACATTCGAAG CAATTGCTTT 
GCCCGCCAGG TGGACAATTA TTTGAGGCCC AATGTAAGTG CCACCACGCA GCTGATCACG 
GACAGCGCAG CCATCAATGT CGCCTTTCGG GCGTATCTCA CATGGCTGGG TTTCCAGGAG 
CCAAACAACG ACTTCACTAA ACTGACCAAG GAGACTCTGC CGGATCTGAA CTACTCAAAC 
ACGGCACTAT TCTTCTTAGC TTATGCCCAA GAGCATTGCA GTCTGGAGGA GGCTCGTCCC 
AGGGATGAGC AAGGGACTCC GGAAGGATAC CTCTATAGTG CACGACAGAA TCACACCTGG 
ACTCGCCTGC TTGTAAACGG ACCTTTGCGA AACTCAGTTG AATTTGCGAG TGAATTTCGG 
TGCCCCATTG GATCACCCAT GAATCCTGCA TCCAAGTGCA TCATATACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nepl14 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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