Nepl13 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nepl13
Entrez Gene ID	42699
Full Name	CG4725 gene product from transcript CG4725-RA
Synonyms	Dmel\CG4725
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 94C4-94C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_142839.4	NP_651096.1	Neprilysin-like 13

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG4723	NP_651097.1
Drosophila melanogaster (fruit fly)	CG4725	NP_651096.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nepl13 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nepl13 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08697	NM_142839.4 Latest version!	Drosophila melanogaster Neprilysin-like 13 (Nepl13), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nepl13 ( NM_142839.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651096.1 Drosophila melanogaster Neprilysin-like 13 (Nepl13), mRNA.

Price & Availability↑

CloneID	OFa08697
Clone ID Related Accession (Same CDS sequence)	NM_142839.4
Accession Version	NM_142839.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Neprilysin-like 13
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_142839.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGAGCTA CCAGAAGCGC TTGGTTGGGC TTTCTGCTCG AGCTGCTACT CCTCTTTGAA 
ATCCAACTTT GCGATGTGAC TGAAGCCCGA CAAAACCATA TGAATCGCAT GCAGATGAGT 
GGAAACTTTC CAGACTCACG TGAGGAGCAA CTGGTGCGCC AAAAAATAGC TACAGATGTG 
CAAAAGTATA TGAACCTGAG CGCCGATCCC TGTACGGATT TCTTCGAGTA TGCCTGCGGT 
CAGTGGGGCC GTTATCACAA GCGACAGCTT CGTCCGGATG AGTTGTCCAC TGCCCAGCAA 
CTGATGGAGA CCAAAATTTC GGAGCAACTG CAGCAGCTTC TCACCCAGCC ACTGCCGCCG 
CAACATCATC CGAACGGATA TAGTGGAGCC AGCATGACGA ATGTTCGAAA AGTGCGAGCC 
TTCTACGAAT CTTGCGTCGC AGTGGAAGCA AATGCCAGTG AGAGACGTCG TTTCCTAATG 
AAGATTCTCA AGGATAACGG AGGATTACGC AACGTTCCCA ACTCCAACTG GCAGCACAAT 
CGTCAGTGGG TACAAACACT GGGCGAACTG AGAAGGAACT ACGGATTGGA CATACTTTTG 
GGTCTGGAAA TCGATCTGAA TCTGCAAACC ATGAAGGGTA ATACCATATA CTTTGGTGAG 
CCCAAGCTCA CCATCATTCC AGCTGAGCAC TGCAATGCCT TGGCAACGCG AGGAGCTCAA 
GTGCGAGATG AGGTCTACGA GCTGGTGCAG CAGCAGGTGA CCGAGAATTT GCGCGATTGG 
TTTGGCATGG ACACCGGAGA GGCGGCTAGA TTTGCAGGAG ATATTATTCG TTTCGAGTTT 
GAGCTGTGCA AACAAATGGG AGAGCAGGAT ATTCAGAAGC CAGAGGAGGA GTTTCCACCC 
GCCATACAGG CGCAAATTTA CGGCGCGCGA TCGCGAACTG GTCAAGAGCG TCTGCGGCGC 
CAGAAAGGAG GAATGACCCT GACCGAGCTC ACCAGCGAGA TGGGAAATCA CTTGGACTTT 
AAAATGCTGG TAGAGGTCAT CCTGGAGAGT CAGTACCCAT CCCAAGTCTA TCTTCGTTCT 
CCGGAATATG TTAAACATGT GGTGAGGACT GTTAAGGCCA ATAACCGCAT CACCGTTGGT 
GGCTACATTC TATACGTGGC CCTGAACGAA CTGAATCAGC CGCCAGAGGA AGCACCTTCA 
CAACGTGCCC GCCAATGCGT CCAGGTGACG CAACGTCTCT TTCCGCAAGT TTTGGGTGAG 
ATGTTCCAGC GCCATGTACA GCGCGATAAT GCAAAACATG ATCTGGACGC CGTTTTCAAC 
GATGTGATCA AGGCGCTCGA GGAGCAGTTC CACGTGGAAT GGATGGACGA GAACGATCGC 
AGGGCGGCCA GAACCAGACT GTCACAATAT CGAGTGTCTC TGCCGGATTA CCAAAGTTTG 
GATCTAACCG ATTTGCAGTT CCAGAAGTCA GAAGACTACT GGCGCCGTTT GGAGATTGCA 
CTAAAGTATC GTTCCCATCA GCAGTTCGAA GCCCTCAGGG GCAACGATTT TGGTCAGGAT 
GCAGACGGTC TGGATGCATT CGAGGTGCGA GCTGCTCTAT CCCCCCGCCA GCAAATGGTC 
CTGGTTGGAT GGGGTCTGCT GCAGGCTCCA TACTACAATT ACTACTACCC AAAGTCTTTG 
AAATATGCGC TATTGGGCCA TCGATTGGCC AGTGCATTGG TTCAGGCATT CGATGACGAG 
GGATGGAACA ATCATCCACA GGCGACGGCC CAGTGGAATG AGGTGACCAT GTCTGGTTAC 
CGAAATGCCA GCGAATGCCA ACGGGCTCAG TACAGTAGTT ACTTGTACAA TGAACCTGGC 
GAATTTCGCA ATGCCACCAG GCTAAGAGAG ATTATAGCCG ATGGCAGTGG TCTCAACTTG 
GCCTTCAACG CGTATCTAGC TTGGCTGGAG CAGCAGGATC ACAAGCTACG TCCACTACTG 
GCGAAGGAAA CGCTGTCGGA GTTAAATTTC ACCAACACCC AGCTATTCTT CATATACTTC 
GCACAGACGC GCTGCTGGGC AAAGGACAAC CAGGATGCCA TCCTGGACTC GATGCCTCTG 
ATGCAGCACA CACCGGAGAG ATGGGATGTC AATGGACCGC TCAGCAACTC CGCGGAATTC 
GGACGAGAGT TCGGCTGTGC CCTGGGCACA CCGATGAATA GTGGCGACAA GTGTTTGGTT 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651096.1
CDS	6..2231
Translation	MRATRSAWLGFLLELLLLFEIQLCDVTEARQNHMNRMQMSGNFPDSREEQLVRQKIATDVQKYMNLSADPCTDFFEYACGQWGRYHKRQLRPDELSTAQQLMETKISEQLQQLLTQPLPPQHHPNGYSGASMTNVRKVRAFYESCVAVEANASERRRFLMKILKDNGGLRNVPNSNWQHNRQWVQTLGELRRNYGLDILLGLEIDLNLQTMKGNTIYFGEPKLTIIPAEHCNALATRGAQVRDEVYELVQQQVTENLRDWFGMDTGEAARFAGDIIRFEFELCKQMGEQDIQKPEEEFPPAIQAQIYGARSRTGQERLRRQKGGMTLTELTSEMGNHLDFKMLVEVILESQYPSQVYLRSPEYVKHVVRTVKANNRITVGGYILYVALNELNQPPEEAPSQRARQCVQVTQRLFPQVLGEMFQRHVQRDNAKHDLDAVFNDVIKALEEQFHVEWMDENDRRAARTRLSQYRVSLPDYQSLDLTDLQFQKSEDYWRRLEIALKYRSHQQFEALRGNDFGQDADGLDAFEVRAALSPRQQMVLVGWGLLQAPYYNYYYPKSLKYALLGHRLASALVQAFDDEGWNNHPQATAQWNEVTMSGYRNASECQRAQYSSYLYNEPGEFRNATRLREIIADGSGLNLAFNAYLAWLEQQDHKLRPLLAKETLSELNFTNTQLFFIYFAQTRCWAKDNQDAILDSMPLMQHTPERWDVNGPLSNSAEFGREFGCALGTPMNSGDKCLVY

Target ORF information:

RefSeq Version	NM_142839.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Neprilysin-like 13 (Nepl13), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142839.4

ORF Insert Sequence:

ATGAGAGCTA CCAGAAGCGC TTGGTTGGGC TTTCTGCTCG AGCTGCTACT CCTCTTTGAA 
ATCCAACTTT GCGATGTGAC TGAAGCCCGA CAAAACCATA TGAATCGCAT GCAGATGAGT 
GGAAACTTTC CAGACTCACG TGAGGAGCAA CTGGTGCGCC AAAAAATAGC TACAGATGTG 
CAAAAGTATA TGAACCTGAG CGCCGATCCC TGTACGGATT TCTTCGAGTA TGCCTGCGGT 
CAGTGGGGCC GTTATCACAA GCGACAGCTT CGTCCGGATG AGTTGTCCAC TGCCCAGCAA 
CTGATGGAGA CCAAAATTTC GGAGCAACTG CAGCAGCTTC TCACCCAGCC ACTGCCGCCG 
CAACATCATC CGAACGGATA TAGTGGAGCC AGCATGACGA ATGTTCGAAA AGTGCGAGCC 
TTCTACGAAT CTTGCGTCGC AGTGGAAGCA AATGCCAGTG AGAGACGTCG TTTCCTAATG 
AAGATTCTCA AGGATAACGG AGGATTACGC AACGTTCCCA ACTCCAACTG GCAGCACAAT 
CGTCAGTGGG TACAAACACT GGGCGAACTG AGAAGGAACT ACGGATTGGA CATACTTTTG 
GGTCTGGAAA TCGATCTGAA TCTGCAAACC ATGAAGGGTA ATACCATATA CTTTGGTGAG 
CCCAAGCTCA CCATCATTCC AGCTGAGCAC TGCAATGCCT TGGCAACGCG AGGAGCTCAA 
GTGCGAGATG AGGTCTACGA GCTGGTGCAG CAGCAGGTGA CCGAGAATTT GCGCGATTGG 
TTTGGCATGG ACACCGGAGA GGCGGCTAGA TTTGCAGGAG ATATTATTCG TTTCGAGTTT 
GAGCTGTGCA AACAAATGGG AGAGCAGGAT ATTCAGAAGC CAGAGGAGGA GTTTCCACCC 
GCCATACAGG CGCAAATTTA CGGCGCGCGA TCGCGAACTG GTCAAGAGCG TCTGCGGCGC 
CAGAAAGGAG GAATGACCCT GACCGAGCTC ACCAGCGAGA TGGGAAATCA CTTGGACTTT 
AAAATGCTGG TAGAGGTCAT CCTGGAGAGT CAGTACCCAT CCCAAGTCTA TCTTCGTTCT 
CCGGAATATG TTAAACATGT GGTGAGGACT GTTAAGGCCA ATAACCGCAT CACCGTTGGT 
GGCTACATTC TATACGTGGC CCTGAACGAA CTGAATCAGC CGCCAGAGGA AGCACCTTCA 
CAACGTGCCC GCCAATGCGT CCAGGTGACG CAACGTCTCT TTCCGCAAGT TTTGGGTGAG 
ATGTTCCAGC GCCATGTACA GCGCGATAAT GCAAAACATG ATCTGGACGC CGTTTTCAAC 
GATGTGATCA AGGCGCTCGA GGAGCAGTTC CACGTGGAAT GGATGGACGA GAACGATCGC 
AGGGCGGCCA GAACCAGACT GTCACAATAT CGAGTGTCTC TGCCGGATTA CCAAAGTTTG 
GATCTAACCG ATTTGCAGTT CCAGAAGTCA GAAGACTACT GGCGCCGTTT GGAGATTGCA 
CTAAAGTATC GTTCCCATCA GCAGTTCGAA GCCCTCAGGG GCAACGATTT TGGTCAGGAT 
GCAGACGGTC TGGATGCATT CGAGGTGCGA GCTGCTCTAT CCCCCCGCCA GCAAATGGTC 
CTGGTTGGAT GGGGTCTGCT GCAGGCTCCA TACTACAATT ACTACTACCC AAAGTCTTTG 
AAATATGCGC TATTGGGCCA TCGATTGGCC AGTGCATTGG TTCAGGCATT CGATGACGAG 
GGATGGAACA ATCATCCACA GGCGACGGCC CAGTGGAATG AGGTGACCAT GTCTGGTTAC 
CGAAATGCCA GCGAATGCCA ACGGGCTCAG TACAGTAGTT ACTTGTACAA TGAACCTGGC 
GAATTTCGCA ATGCCACCAG GCTAAGAGAG ATTATAGCCG ATGGCAGTGG TCTCAACTTG 
GCCTTCAACG CGTATCTAGC TTGGCTGGAG CAGCAGGATC ACAAGCTACG TCCACTACTG 
GCGAAGGAAA CGCTGTCGGA GTTAAATTTC ACCAACACCC AGCTATTCTT CATATACTTC 
GCACAGACGC GCTGCTGGGC AAAGGACAAC CAGGATGCCA TCCTGGACTC GATGCCTCTG 
ATGCAGCACA CACCGGAGAG ATGGGATGTC AATGGACCGC TCAGCAACTC CGCGGAATTC 
GGACGAGAGT TCGGCTGTGC CCTGGGCACA CCGATGAATA GTGGCGACAA GTGTTTGGTT 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nepl13 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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