lbe cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	lbe
Entrez Gene ID	42542
Full Name	ladybird early
Synonyms	CG6545,Dmel\CG6545,Hox11-D125,Lb,Lbe,NK?,lb
General protein information	Preferred Names ladybird early Names CG6545 gene product from transcript CG6545-RA CG6545-PA lady-bird-early ladybird lbe-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 93E1-93E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_079711.3	NP_524435.2	ladybird early

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003672	XP_001230938.3
Drosophila melanogaster (fruit fly)	lbe	NP_524435.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

here we examine the interactions between Msk and the Wg pathway in regulation of the AMP pool size. We find that a myoblast-specific reduction of Msk results in the absence of Vg expression and a complete loss of the Wg pathway readout beta-catenin/Armadillo (Arm). Moreover, msk RNA interference knockdown abolishes expression of the Wg target Ladybird (Lbe) in leg disc myoblasts.
Title: Adult Muscle Formation Requires

Lbe role in the neuronal cell fate specification
Title: Neuronal Cell Fate Specification by the Convergence of Different Spatiotemporal Cues on a Common Terminal Selector Cascade.

evolution of lbe and lbl
Title: Complex interplay of evolutionary forces in the ladybird homeobox genes of Drosophila melanogaster.

in developing Drosophila cardiac progenitor cells, the Eve precursors next to the Hh stripe are distinguished from more distant Lbe precursors by locally augmenting Ras signaling via elevating rho transcripts
Title: Hedgehog and RAS pathways cooperate in the anterior-posterior specification and positioning of cardiac progenitor cells.

Data show that the homeobox genes ladybird early and ladybird late are expressed in subsets of cells deriving from neuroblasts NB 5-3 and NB 5-6 and are essential for their correct development.
Title: The ladybird homeobox genes are essential for the specification of a subpopulation of neural cells.

The following lbe gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lbe cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05602	NM_079711.3 Latest version!	Drosophila melanogaster ladybird early (lbe), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lbe ( NM_079711.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524435.2 Drosophila melanogaster ladybird early (lbe), mRNA.

Price & Availability↑

CloneID	OFa05602
Clone ID Related Accession (Same CDS sequence)	NM_079711.3
Accession Version	NM_079711.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ladybird early
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079711.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTCTGCC CTCCAACCAT GCGTCCGGCC AGTCCCGCCG AATCCGAAAT CTCCGTGGGC 
GGAGCCCCCA GCCCGCTGCC CACGCAGCAT CATACGCATC CGCACTCGCA TCCTCATCCG 
CTGCAGCATC CACGGGCCAG CACCATTGAT CTGCTGCAGC AGCAGCAGCT GCTGATGCAG 
CACCATGCCG CAGCAGCCGC CGCAGCAGCT GCAGCCGCCT CCGGACTCAC CCGGAGTGCC 
GTCGGCAATT TGCCGGAGGA CTACTTCCAT CCGCTGAAGC GCCTGCGCAT GTCCTCGTCC 
TCCTCGGAGC CACGAGACCA CACGCCCAGT CCACCGTCAG CAGTTCCGGA GCCCCAGACC 
AACCAGACTA CCAAGTCGGC CATCGAGGGG GTCAAGAGCT TCTCCATCGC GGACATACTG 
GGTCACTCGG AGAAGCAGCG CGAGGAGTCC GTTTCCCCGC CCCCAAATGC AAACCTCCTG 
GCTCCACCTG CCTCCCGGCC CATTGCCCCC TCGGGCGGAT TACTGCAACC ACGCACGGAA 
CCGTTGGATG TCCACCCGGC CGCTGCTGCT GCCATGCTCC TGCCAAGCGG ACAGATTGTT 
CGGCCGTGGG ATCACCTCCT GGGGCCGACC ATGCCCGTCC GCCCCTTCAT CCCCTCCGCC 
CTGCTGCACT ACGAGCAGCG CCTGGCTCTG GACTACCATC GCCAGTTGCA GGAACACTTT 
AACGCCCAGG CCCAGCTCCT TCGCCACATG GGCATGAATC CCGCGATCAT CGCTTCCGAG 
GACGGCAGCT CGGAGCGTTC CCAGCGATCG AGCAGCAGCA ATGGCAGCAC CGAATGCTGC 
AGCCCACGGC AGGCGGAAAA ACTTGAAAAA CTAACCACCC AAGAGGGAAG TGAGGAAGCT 
CAGAAGAAGA AATCAGAGGA GCAGCCAACG GGTTCAGGAA AGTCAAACGG AGACACTCCT 
CTGGATGCTC TCTTCCAGAT GACCACAAAG GACTTTGACG AGTCGCAAGA TAAATCCCAC 
TTGGACATCT TCTCGAACAG GCCGCAGCCG AAGAAGAAGC GCAAGTCGCG CACCGCCTTC 
ACCAACCACC AGATCTTCGA GCTGGAGAAG CGATTTCTCT ACCAGAAGTA CCTGTCGCCA 
GCGGACCGCG ATGAGATCGC CGCCTCACTG GGACTGTCGA ACGCCCAGGT GATCACCTGG 
TTCCAGAACC GGCGAGCCAA GCAGAAGCGG GACATCGAGG AGCTGAAGAA GGACTTCGAT 
AGCGTCAAGG TCTTCTCCGC CCACAAGTCC TTCCTGGAGA ACGTGAACGA CCTAAGCATC 
CTCAAGAAGA AGCCCATGCA TGAGTCAGAT ATGGTGGGCC TGGCGGCTGC GGCAGCCGCT 
GCCGGAATGG TGGTTCCCGT GCCTGGATCC GTGCCAATGG GAGGCGCTCC GCCCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524435.2
CDS	445..1884
Translation	MLCPPTMRPASPAESEISVGGAPSPLPTQHHTHPHSHPHPLQHPRASTIDLLQQQQLLMQHHAAAAAAAAAAASGLTRSAVGNLPEDYFHPLKRLRMSSSSSEPRDHTPSPPSAVPEPQTNQTTKSAIEGVKSFSIADILGHSEKQREESVSPPPNANLLAPPASRPIAPSGGLLQPRTEPLDVHPAAAAAMLLPSGQIVRPWDHLLGPTMPVRPFIPSALLHYEQRLALDYHRQLQEHFNAQAQLLRHMGMNPAIIASEDGSSERSQRSSSSNGSTECCSPRQAEKLEKLTTQEGSEEAQKKKSEEQPTGSGKSNGDTPLDALFQMTTKDFDESQDKSHLDIFSNRPQPKKKRKSRTAFTNHQIFELEKRFLYQKYLSPADRDEIAASLGLSNAQVITWFQNRRAKQKRDIEELKKDFDSVKVFSAHKSFLENVNDLSILKKKPMHESDMVGLAAAAAAAGMVVPVPGSVPMGGAPPK

Target ORF information:

RefSeq Version	NM_079711.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ladybird early (lbe), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079711.3

ORF Insert Sequence:

ATGCTCTGCC CTCCAACCAT GCGTCCGGCC AGTCCCGCCG AATCCGAAAT CTCCGTGGGC 
GGAGCCCCCA GCCCGCTGCC CACGCAGCAT CATACGCATC CGCACTCGCA TCCTCATCCG 
CTGCAGCATC CACGGGCCAG CACCATTGAT CTGCTGCAGC AGCAGCAGCT GCTGATGCAG 
CACCATGCCG CAGCAGCCGC CGCAGCAGCT GCAGCCGCCT CCGGACTCAC CCGGAGTGCC 
GTCGGCAATT TGCCGGAGGA CTACTTCCAT CCGCTGAAGC GCCTGCGCAT GTCCTCGTCC 
TCCTCGGAGC CACGAGACCA CACGCCCAGT CCACCGTCAG CAGTTCCGGA GCCCCAGACC 
AACCAGACTA CCAAGTCGGC CATCGAGGGG GTCAAGAGCT TCTCCATCGC GGACATACTG 
GGTCACTCGG AGAAGCAGCG CGAGGAGTCC GTTTCCCCGC CCCCAAATGC AAACCTCCTG 
GCTCCACCTG CCTCCCGGCC CATTGCCCCC TCGGGCGGAT TACTGCAACC ACGCACGGAA 
CCGTTGGATG TCCACCCGGC CGCTGCTGCT GCCATGCTCC TGCCAAGCGG ACAGATTGTT 
CGGCCGTGGG ATCACCTCCT GGGGCCGACC ATGCCCGTCC GCCCCTTCAT CCCCTCCGCC 
CTGCTGCACT ACGAGCAGCG CCTGGCTCTG GACTACCATC GCCAGTTGCA GGAACACTTT 
AACGCCCAGG CCCAGCTCCT TCGCCACATG GGCATGAATC CCGCGATCAT CGCTTCCGAG 
GACGGCAGCT CGGAGCGTTC CCAGCGATCG AGCAGCAGCA ATGGCAGCAC CGAATGCTGC 
AGCCCACGGC AGGCGGAAAA ACTTGAAAAA CTAACCACCC AAGAGGGAAG TGAGGAAGCT 
CAGAAGAAGA AATCAGAGGA GCAGCCAACG GGTTCAGGAA AGTCAAACGG AGACACTCCT 
CTGGATGCTC TCTTCCAGAT GACCACAAAG GACTTTGACG AGTCGCAAGA TAAATCCCAC 
TTGGACATCT TCTCGAACAG GCCGCAGCCG AAGAAGAAGC GCAAGTCGCG CACCGCCTTC 
ACCAACCACC AGATCTTCGA GCTGGAGAAG CGATTTCTCT ACCAGAAGTA CCTGTCGCCA 
GCGGACCGCG ATGAGATCGC CGCCTCACTG GGACTGTCGA ACGCCCAGGT GATCACCTGG 
TTCCAGAACC GGCGAGCCAA GCAGAAGCGG GACATCGAGG AGCTGAAGAA GGACTTCGAT 
AGCGTCAAGG TCTTCTCCGC CCACAAGTCC TTCCTGGAGA ACGTGAACGA CCTAAGCATC 
CTCAAGAAGA AGCCCATGCA TGAGTCAGAT ATGGTGGGCC TGGCGGCTGC GGCAGCCGCT 
GCCGGAATGG TGGTTCCCGT GCCTGGATCC GTGCCAATGG GAGGCGCTCC GCCCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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lbe cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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