TFAM cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	TFAM
Entrez Gene ID	42433
Full Name	mitochondrial transcription factor A
Synonyms	CG4217,D-mtTFA,Dmel\CG4217,MTTFA,Tfam,d-TFAM,d-mttfa,d-tfam,mtTFA,mttfa,tfam
General protein information	Preferred Names mitochondrial transcription factor A Names CG4217 gene product from transcript CG4217-RB CG4217-PA CG4217-PB TFAM-PA TFAM-PB mitochondiral transcription fctor a mitochondrial transcription factor
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 92F2-92F2

mRNA and Protein(s)

mRNA	Protein	Name
NM_079691.3	NP_524415.1	mitochondrial transcription factor A, isoform A
NM_169906.2	NP_732527.1	mitochondrial transcription factor A, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	TFAM	NP_003192.1
Danio rerio (zebrafish)	tfam	NP_001070857.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008499	XP_316944.3
Xenopus tropicalis (tropical clawed frog)	tfam	XP_002937148.2
Canis lupus familiaris (dog)	TFAM	XP_546107.2
Mus musculus (house mouse)	Tfam	NP_033386.1
Rattus norvegicus (Norway rat)	Tfam	NP_112616.1
	TFAM	XP_001097656.1
Bos taurus (cattle)	TFAM	NP_001029188.2
Gallus gallus (chicken)	TFAM	NP_989431.1
Pan troglodytes (chimpanzee)	TFAM	NP_001181871.1
Drosophila melanogaster (fruit fly)	TFAM	NP_524415.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The cAMP phosphodiesterase Prune localizes to the mitochondrial matrix and promotes mtDNA replication by stabilizing TFAM.
Title: The cAMP phosphodiesterase Prune localizes to the mitochondrial matrix and promotes mtDNA replication by stabilizing TFAM.

The present results clearly show the effects of TFAM overexpression on the lifespan of Drosophila under both standard conditions and oxidative stress conditions, and our findings contribute to the understanding of the physiological mechanisms involving TFAM in mitochondria.
Title: Effects of overexpression of mitochondrial transcription factor A on lifespan and oxidative stress response in Drosophila melanogaster.

Lon regulates mitochondrial transcription by stabilizing the mitochondrial TFAMmtDNA ratio via selective degradation of TFAM.
Title: Mitochondrial Lon protease regulates mitochondrial DNA copy number and transcription by selective degradation of mitochondrial transcription factor A (TFAM).

Mitochondrial transcription factor A is detected in Drosophila brain throughout the developmental process, as well as in non-proliferating tissues, such as flight muscle and cardia, and is also found in spermatids of imagos.
Title: Spatio-temporal expression of Drosophila mitochondrial transcription factor A during development.

The functions of mtTFA were in relation to mitochondrial biogenesis of Drosophila melanogaster.
Title: Drosophila mitochondrial transcription factor A: characterization of its cDNA and expression pattern during development.

The following TFAM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TFAM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05483	NM_169906.2 Latest version!	Drosophila melanogaster mitochondrial transcription factor A (TFAM), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa05484	NM_079691.3 Latest version!	Drosophila melanogaster mitochondrial transcription factor A (TFAM), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TFAM ( NM_169906.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732527.1 Drosophila melanogaster mitochondrial transcription factor A (TFAM), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa05483
Clone ID Related Accession (Same CDS sequence)	NM_169906.2
Accession Version	NM_169906.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial transcription factor A, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169906.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATCTACA CCACAACACT GATGTCCTCG CGCGGCGGCC TCATCGGCTC GCTGATCAAC 
AAAGTCAGGC AAGTTGTAGC CAAAGATTGG CCACCCAGTG ATCCATCTCG TGCCAATGAC 
ACCGACCACC GACCCCTCAC ACCCCCTAGG CCCCTAGCAG CCGCCAGCAT CAGCAACACT 
CCGGCGGTGC CGTCGAAGAC CCTGGAGGAG CAGTTGGGCC TGCCGCCGCG ACCAAAGAAA 
CCGCTGACTC CCTACTTTCG CTTCATGCGG GAGCAGCGGC CCAAGCTGAA GGCTGCCAAT 
CCCCAGATTA CCACCGTCGA GGTGGTGCGC CAGCTGTCTA AGAACTGGTC CGATGCCGAT 
GCGCAGCTGA AGGAGCGCCT GCAGGCCGAG TTCAAGCGGG ACCAACAAAT CTACGTGGAG 
GAGCGAACAA AGTACGATGC CACACTCACG GAGGAGCAGC GGGCCGAGAT CAAGCAGCTC 
AAGCAGGACC TCGTTGACGC CAAGGAGCGC CGCCAGCTGC GCAAGCGGGT CAAGGAGCTG 
GGGCGACCCA AAAAGCCCGC TTCGGCCTTC CTGCGATTCA TCGCCAGCGA ACGTATCAAC 
ACTCCGCAGG GCGACAAGCA AACCTACCGC GAGTGGCACC AAAAGACCAC CGCCAAGTGG 
ACTCGCCTTT CCGACTCCGA GAAGGAGGTC TACATGCAGG AGTCGCGCAA GGAGATGGAG 
CTCTACAGGA AAGCGATTTC CGTTTGGGAG GAGAAGATGA TCCGCCTGGG CCACATCGAC 
GTGGTGCGTC ACGGAAATCT TATCGATCCA CCTGAGCCAA AGCCCCGCAA GACGCTGGCC 
TCCAAAGATA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732527.1
CDS	138..992
Translation	MIYTTTLMSSRGGLIGSLINKVRQVVAKDWPPSDPSRANDTDHRPLTPPRPLAAASISNTPAVPSKTLEEQLGLPPRPKKPLTPYFRFMREQRPKLKAANPQITTVEVVRQLSKNWSDADAQLKERLQAEFKRDQQIYVEERTKYDATLTEEQRAEIKQLKQDLVDAKERRQLRKRVKELGRPKKPASAFLRFIASERINTPQGDKQTYREWHQKTTAKWTRLSDSEKEVYMQESRKEMELYRKAISVWEEKMIRLGHIDVVRHGNLIDPPEPKPRKTLASKDI

Target ORF information:

RefSeq Version	NM_169906.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial transcription factor A (TFAM), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169906.2

ORF Insert Sequence:

ATGATCTACA CCACAACACT GATGTCCTCG CGCGGCGGCC TCATCGGCTC GCTGATCAAC 
AAAGTCAGGC AAGTTGTAGC CAAAGATTGG CCACCCAGTG ATCCATCTCG TGCCAATGAC 
ACCGACCACC GACCCCTCAC ACCCCCTAGG CCCCTAGCAG CCGCCAGCAT CAGCAACACT 
CCGGCGGTGC CGTCGAAGAC CCTGGAGGAG CAGTTGGGCC TGCCGCCGCG ACCAAAGAAA 
CCGCTGACTC CCTACTTTCG CTTCATGCGG GAGCAGCGGC CCAAGCTGAA GGCTGCCAAT 
CCCCAGATTA CCACCGTCGA GGTGGTGCGC CAGCTGTCTA AGAACTGGTC CGATGCCGAT 
GCGCAGCTGA AGGAGCGCCT GCAGGCCGAG TTCAAGCGGG ACCAACAAAT CTACGTGGAG 
GAGCGAACAA AGTACGATGC CACACTCACG GAGGAGCAGC GGGCCGAGAT CAAGCAGCTC 
AAGCAGGACC TCGTTGACGC CAAGGAGCGC CGCCAGCTGC GCAAGCGGGT CAAGGAGCTG 
GGGCGACCCA AAAAGCCCGC TTCGGCCTTC CTGCGATTCA TCGCCAGCGA ACGTATCAAC 
ACTCCGCAGG GCGACAAGCA AACCTACCGC GAGTGGCACC AAAAGACCAC CGCCAAGTGG 
ACTCGCCTTT CCGACTCCGA GAAGGAGGTC TACATGCAGG AGTCGCGCAA GGAGATGGAG 
CTCTACAGGA AAGCGATTTC CGTTTGGGAG GAGAAGATGA TCCGCCTGGG CCACATCGAC 
GTGGTGCGTC ACGGAAATCT TATCGATCCA CCTGAGCCAA AGCCCCGCAA GACGCTGGCC 
TCCAAAGATA TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TFAM ( NM_079691.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524415.1 Drosophila melanogaster mitochondrial transcription factor A (TFAM), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa05484
Clone ID Related Accession (Same CDS sequence)	NM_079691.3
Accession Version	NM_079691.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial transcription factor A, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079691.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATCTACA CCACAACACT GATGTCCTCG CGCGGCGGCC TCATCGGCTC GCTGATCAAC 
AAAGTCAGGC CCCTAGCAGC CGCCAGCATC AGCAACACTC CGGCGGTGCC GTCGAAGACC 
CTGGAGGAGC AGTTGGGCCT GCCGCCGCGA CCAAAGAAAC CGCTGACTCC CTACTTTCGC 
TTCATGCGGG AGCAGCGGCC CAAGCTGAAG GCTGCCAATC CCCAGATTAC CACCGTCGAG 
GTGGTGCGCC AGCTGTCTAA GAACTGGTCC GATGCCGATG CGCAGCTGAA GGAGCGCCTG 
CAGGCCGAGT TCAAGCGGGA CCAACAAATC TACGTGGAGG AGCGAACAAA GTACGATGCC 
ACACTCACGG AGGAGCAGCG GGCCGAGATC AAGCAGCTCA AGCAGGACCT CGTTGACGCC 
AAGGAGCGCC GCCAGCTGCG CAAGCGGGTC AAGGAGCTGG GGCGACCCAA AAAGCCCGCT 
TCGGCCTTCC TGCGATTCAT CGCCAGCGAA CGTATCAACA CTCCGCAGGG CGACAAGCAA 
ACCTACCGCG AGTGGCACCA AAAGACCACC GCCAAGTGGA CTCGCCTTTC CGACTCCGAG 
AAGGAGGTCT ACATGCAGGA GTCGCGCAAG GAGATGGAGC TCTACAGGAA AGCGATTTCC 
GTTTGGGAGG AGAAGATGAT CCGCCTGGGC CACATCGACG TGGTGCGTCA CGGAAATCTT 
ATCGATCCAC CTGAGCCAAA GCCCCGCAAG ACGCTGGCCT CCAAAGATAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524415.1
CDS	138..911
Translation	MIYTTTLMSSRGGLIGSLINKVRPLAAASISNTPAVPSKTLEEQLGLPPRPKKPLTPYFRFMREQRPKLKAANPQITTVEVVRQLSKNWSDADAQLKERLQAEFKRDQQIYVEERTKYDATLTEEQRAEIKQLKQDLVDAKERRQLRKRVKELGRPKKPASAFLRFIASERINTPQGDKQTYREWHQKTTAKWTRLSDSEKEVYMQESRKEMELYRKAISVWEEKMIRLGHIDVVRHGNLIDPPEPKPRKTLASKDI

Target ORF information:

RefSeq Version	NM_079691.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial transcription factor A (TFAM), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079691.3

ORF Insert Sequence:

ATGATCTACA CCACAACACT GATGTCCTCG CGCGGCGGCC TCATCGGCTC GCTGATCAAC 
AAAGTCAGGC CCCTAGCAGC CGCCAGCATC AGCAACACTC CGGCGGTGCC GTCGAAGACC 
CTGGAGGAGC AGTTGGGCCT GCCGCCGCGA CCAAAGAAAC CGCTGACTCC CTACTTTCGC 
TTCATGCGGG AGCAGCGGCC CAAGCTGAAG GCTGCCAATC CCCAGATTAC CACCGTCGAG 
GTGGTGCGCC AGCTGTCTAA GAACTGGTCC GATGCCGATG CGCAGCTGAA GGAGCGCCTG 
CAGGCCGAGT TCAAGCGGGA CCAACAAATC TACGTGGAGG AGCGAACAAA GTACGATGCC 
ACACTCACGG AGGAGCAGCG GGCCGAGATC AAGCAGCTCA AGCAGGACCT CGTTGACGCC 
AAGGAGCGCC GCCAGCTGCG CAAGCGGGTC AAGGAGCTGG GGCGACCCAA AAAGCCCGCT 
TCGGCCTTCC TGCGATTCAT CGCCAGCGAA CGTATCAACA CTCCGCAGGG CGACAAGCAA 
ACCTACCGCG AGTGGCACCA AAAGACCACC GCCAAGTGGA CTCGCCTTTC CGACTCCGAG 
AAGGAGGTCT ACATGCAGGA GTCGCGCAAG GAGATGGAGC TCTACAGGAA AGCGATTTCC 
GTTTGGGAGG AGAAGATGAT CCGCCTGGGC CACATCGACG TGGTGCGTCA CGGAAATCTT 
ATCGATCCAC CTGAGCCAAA GCCCCGCAAG ACGCTGGCCT CCAAAGATAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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TFAM cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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