ninaE cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ninaE
Entrez Gene ID	42367
Full Name	neither inactivation nor afterpotential E
Synonyms	143283_at,1F9,BEST:GH11778,CG4550,DMELRH1,DRh1,Dm Rh1,DmRh1,Dmel\CG4550,FBgn0002940,Nina E,NinaE,R,RH1,Rh,Rh-1,Rh1,Rh1(ninaE),Rh1/ninaE,Rhodopsin-1,opsin,ora,rh1,rh1/ninaE,rhodopsin
General protein information	Preferred Names neither inactivation nor afterpotential E Names CG4550 gene product from transcript CG4550-RA CG4550-PA ninaE-PA rhodopsin rhodopsin 1 rhodopsin-1 rhodopsin1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 92B4-92B4\|3-66.4 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_079683.3	NP_524407.1	neither inactivation nor afterpotential E

Pathways from BioSystems

Homologies

Homo sapiens (human)	OPN4	NP_001025186.1
Pan troglodytes (chimpanzee)	OPN4	XP_001135445.1
Rattus norvegicus (Norway rat)	Opn4	NP_620215.1
Gallus gallus (chicken)	OPN4	NP_001038118.1
Drosophila melanogaster (fruit fly)	ninaE	NP_524407.1
	OPN4	XP_001088248.2
Xenopus tropicalis (tropical clawed frog)	LOC100491785	XP_002937616.1
Canis lupus familiaris (dog)	OPN4	XP_005619497.1
Bos taurus (cattle)	OPN4	NP_001179328.1
Mus musculus (house mouse)	Opn4	NP_001122071.1
Drosophila melanogaster (fruit fly)	Rh2	NP_524398.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Fmr1 protein associates with ninaE mRNA and represses its translation.
Title: Bidirectional regulation of fragile X mental retardation protein phosphorylation controls rhodopsin homoeostasis.

Organisms use multiple strategies to maximize visual capabilities in different light conditions. Many invertebrates show a daily cycle of shedding the photoreceptor's rhabdomeric membranes at dawn and rebuilding these during the following night. We show here that the Aedes aegypti mosquito possesses two distinct light-driven cellular signaling processes for modulating rhodopsin content during this cycle.
Title: Light-Driven Processes Control Both Rhodopsin Maturation and Recycling in Mosquito Photoreceptors.

Data show that site-directed mutagenesis of conserved residues has only modest effects on Rhodopsin 1 absorption.
Title: Analysis of Conserved Glutamate and Aspartate Residues in Drosophila Rhodopsin 1 and Their Influence on Spectral Tuning.

Data indicate that mutations in Golgi SNARE protein gos28 lead to defective rhodopsin (Rh1) trafficking.
Title: The Gos28 SNARE protein mediates intra-Golgi transport of rhodopsin and is required for photoreceptor survival.

GPI biosynthesis is essential for rhodopsin sorting at the trans-Golgi network in Drosophila photoreceptors
Title: GPI biosynthesis is essential for rhodopsin sorting at the trans-Golgi network in Drosophila photoreceptors.

The following ninaE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ninaE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08540	NM_079683.3 Latest version!	Drosophila melanogaster neither inactivation nor afterpotential E (ninaE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ninaE ( NM_079683.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524407.1 Drosophila melanogaster neither inactivation nor afterpotential E (ninaE), mRNA.

Price & Availability↑

CloneID	OFa08540
Clone ID Related Accession (Same CDS sequence)	NM_079683.3
Accession Version	NM_079683.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neither inactivation nor afterpotential E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_079683.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAGCT TTGCAGTAGC AGCCGCGCAA CTGGGGCCAC ACTTTGCCCC CCTGTCCAAT 
GGATCGGTGG TGGACAAGGT CACGCCCGAC ATGGCCCACC TGATCAGCCC GTACTGGAAC 
CAGTTCCCCG CCATGGACCC CATTTGGGCC AAGATCCTGA CCGCCTACAT GATCATGATC 
GGCATGATTT CCTGGTGCGG AAATGGCGTG GTGATCTACA TATTCGCCAC CACCAAATCA 
CTGCGCACGC CCGCTAACCT GCTGGTCATC AACCTGGCCA TCTCCGACTT TGGCATCATG 
ATCACAAACA CGCCCATGAT GGGCATAAAT CTGTATTTCG AGACCTGGGT GCTCGGACCC 
ATGATGTGCG ACATATACGC CGGACTGGGC TCGGCCTTTG GCTGCAGCTC CATCTGGTCC 
ATGTGCATGA TCTCCCTGGA TCGCTACCAA GTGATCGTCA AGGGCATGGC CGGCCGGCCG 
ATGACCATTC CGCTGGCCCT GGGCAAGATT GCCTACATCT GGTTCATGTC GAGCATTTGG 
TGCCTGGCGC CCGCCTTCGG CTGGAGCAGG TATGTGCCGG AGGGTAACCT GACCTCGTGC 
GGTATTGACT ACTTGGAACG CGACTGGAAC CCACGCTCAT ACCTGATCTT CTACTCCATC 
TTCGTGTACT ATATTCCGCT GTTCCTGATC TGCTACTCTT ACTGGTTCAT CATTGCTGCT 
GTCTCCGCCC ACGAGAAGGC CATGCGCGAG CAGGCCAAGA AGATGAATGT CAAGTCCCTC 
CGCTCCTCTG AGGATGCCGA GAAGAGCGCC GAGGGCAAGC TGGCCAAGGT GGCTCTGGTC 
ACCATCACGC TGTGGTTCAT GGCGTGGACA CCATACCTGG TCATCAACTG CATGGGACTG 
TTCAAGTTCG AGGGCCTTAC ACCACTGAAT ACCATTTGGG GAGCTTGCTT CGCCAAATCG 
GCCGCCTGCT ACAATCCAAT TGTATACGGC ATCAGCCATC CGAAATATCG CCTGGCCCTC 
AAGGAGAAGT GTCCTTGCTG CGTCTTTGGC AAGGTCGACG ATGGCAAATC GAGCGATGCC 
CAATCGCAGG CCACCGCCAG CGAGGCCGAG TCCAAGGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524407.1
CDS	177..1298
Translation	MESFAVAAAQLGPHFAPLSNGSVVDKVTPDMAHLISPYWNQFPAMDPIWAKILTAYMIMIGMISWCGNGVVIYIFATTKSLRTPANLLVINLAISDFGIMITNTPMMGINLYFETWVLGPMMCDIYAGLGSAFGCSSIWSMCMISLDRYQVIVKGMAGRPMTIPLALGKIAYIWFMSSIWCLAPAFGWSRYVPEGNLTSCGIDYLERDWNPRSYLIFYSIFVYYIPLFLICYSYWFIIAAVSAHEKAMREQAKKMNVKSLRSSEDAEKSAEGKLAKVALVTITLWFMAWTPYLVINCMGLFKFEGLTPLNTIWGACFAKSAACYNPIVYGISHPKYRLALKEKCPCCVFGKVDDGKSSDAQSQATASEAESKA

Target ORF information:

RefSeq Version	NM_079683.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neither inactivation nor afterpotential E (ninaE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079683.3

ORF Insert Sequence:

ATGGAGAGCT TTGCAGTAGC AGCCGCGCAA CTGGGGCCAC ACTTTGCCCC CCTGTCCAAT 
GGATCGGTGG TGGACAAGGT CACGCCCGAC ATGGCCCACC TGATCAGCCC GTACTGGAAC 
CAGTTCCCCG CCATGGACCC CATTTGGGCC AAGATCCTGA CCGCCTACAT GATCATGATC 
GGCATGATTT CCTGGTGCGG AAATGGCGTG GTGATCTACA TATTCGCCAC CACCAAATCA 
CTGCGCACGC CCGCTAACCT GCTGGTCATC AACCTGGCCA TCTCCGACTT TGGCATCATG 
ATCACAAACA CGCCCATGAT GGGCATAAAT CTGTATTTCG AGACCTGGGT GCTCGGACCC 
ATGATGTGCG ACATATACGC CGGACTGGGC TCGGCCTTTG GCTGCAGCTC CATCTGGTCC 
ATGTGCATGA TCTCCCTGGA TCGCTACCAA GTGATCGTCA AGGGCATGGC CGGCCGGCCG 
ATGACCATTC CGCTGGCCCT GGGCAAGATT GCCTACATCT GGTTCATGTC GAGCATTTGG 
TGCCTGGCGC CCGCCTTCGG CTGGAGCAGG TATGTGCCGG AGGGTAACCT GACCTCGTGC 
GGTATTGACT ACTTGGAACG CGACTGGAAC CCACGCTCAT ACCTGATCTT CTACTCCATC 
TTCGTGTACT ATATTCCGCT GTTCCTGATC TGCTACTCTT ACTGGTTCAT CATTGCTGCT 
GTCTCCGCCC ACGAGAAGGC CATGCGCGAG CAGGCCAAGA AGATGAATGT CAAGTCCCTC 
CGCTCCTCTG AGGATGCCGA GAAGAGCGCC GAGGGCAAGC TGGCCAAGGT GGCTCTGGTC 
ACCATCACGC TGTGGTTCAT GGCGTGGACA CCATACCTGG TCATCAACTG CATGGGACTG 
TTCAAGTTCG AGGGCCTTAC ACCACTGAAT ACCATTTGGG GAGCTTGCTT CGCCAAATCG 
GCCGCCTGCT ACAATCCAAT TGTATACGGC ATCAGCCATC CGAAATATCG CCTGGCCCTC 
AAGGAGAAGT GTCCTTGCTG CGTCTTTGGC AAGGTCGACG ATGGCAAATC GAGCGATGCC 
CAATCGCAGG CCACCGCCAG CGAGGCCGAG TCCAAGGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ninaE cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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