14-3-3epsilon cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	14-3-3epsilon
Entrez Gene ID	42186
Full Name	CG31196 gene product from transcript CG31196-RD
Synonyms	14-3-3,14-3-3 epsilon,14-3-3-e,14-3-3-epsilon,14-3-30epsilon,14-3-3EPSILON,14-3-3e,14-3-3omicron,14.3.3 epsilon,1433epsilon,143E_DROME,CG31196,CT24092,D14-3-3e,D14-3-3epsilon,Dmel\CG31196,EK3-5,PAR-5,PAR5,Par-5,SR3-9,Su(Raf)3B,anon-WO0172774.141,anon-WO02059370.52,d14-3-3epsilon,e-14-3-3,eps,l(3)j2B10,par-5
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 90F10-90F10

mRNA and Protein(s)

mRNA	Protein	Name
NM_169798.3	NP_732311.1	14-3-3epsilon, isoform D
NM_169797.3	NP_732310.1	14-3-3epsilon, isoform B
NM_169799.2	NP_732312.1	14-3-3epsilon, isoform C
NM_169796.2	NP_732309.1	14-3-3epsilon, isoform A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	YWHAE	NP_001233297.1
Saccharomyces cerevisiae (baker's yeast)	BMH1	NP_011104.3
Neurospora crassa	NCU02806	XP_964462.1
Arabidopsis thaliana (thale cress)	GRF3	NP_568557.1
Oryza sativa (rice)	Os04g0462500	NP_001053003.1
Oryza sativa (rice)	Os08g0430500	NP_001061856.1
Mus musculus (house mouse)	Ywhae	NP_033562.3
Arabidopsis thaliana (thale cress)	GRF5	NP_568325.1
Xenopus tropicalis (tropical clawed frog)	ywhae	NP_001008156.1
Homo sapiens (human)	YWHAE	NP_006752.1
Bos taurus (cattle)	YWHAE	NP_776916.1
Drosophila melanogaster (fruit fly)	14-3-3epsilon	NP_732311.1
Schizosaccharomyces pombe (fission yeast)	rad25	NP_594247.1
Danio rerio (zebrafish)	ywhae2	NP_001013359.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001151	XP_322009.2
Saccharomyces cerevisiae (baker's yeast)	BMH2	NP_010384.3
Eremothecium gossypii	AGOS_AGR107C	NP_986773.1
Arabidopsis thaliana (thale cress)	GRF7	NP_566174.1
Oryza sativa (rice)	Os02g0580300	NP_001047234.1
Canis lupus familiaris (dog)	YWHAE	XP_854358.1
Rattus norvegicus (Norway rat)	Ywhae	NP_113791.1
Gallus gallus (chicken)	YWHAE	NP_001006219.1
Kluyveromyces lactis	KLLA0F12100g	XP_455629.1
Magnaporthe oryzae (rice blast fungus)	MGG_01588	XP_003714567.1
Oryza sativa (rice)	Os03g0710800	NP_001051051.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Khc73 stalk/14-3-3/NudE pathway defines a physical connection that coordinates the activities of multiple motor proteins to precisely position the spindle.
Title: A NudE/14-3-3 pathway coordinates dynein and the kinesin Khc73 to position the mitotic spindle.

14-3-3epsilon acts as a biochemical control point for axon guidance in Drosophila, silencing Plexin A repulsive axon guidance and regulating a semaphorin repulsion/integrin adhesion switch.
Title: 14-3-3? couples protein kinase A to semaphorin signaling and silences plexin RasGAP-mediated axonal repulsion.

role of 14-3-3epsilon in germ cell migration
Title: 14-3-3? Is required for germ cell migration in Drosophila.

We conclude that 14-3-3epsilon is required for Rab11-positive vesicle function, which in turn enables antimicrobial peptide secretion during an innate immune response.
Title: Drosophila 14-3-3? has a crucial role in anti-microbial peptide secretion and innate immunity.

Hpo signaling inhibited Yki nuclear localization and activity by phosphorylating Yki and both isoforms of 14-3-3, 14-3-3varepsilon and 14-3-3zeta, regulate Yki activity through modulating its subcellular localization.
Title: Hippo signaling regulates Yorkie nuclear localization and activity through 14-3-3 dependent and independent mechanisms.

The following 14-3-3epsilon gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the 14-3-3epsilon cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa08479	NM_169797.3 Latest version!	Drosophila melanogaster 14-3-3epsilon, transcript variant B (14-3-3epsilon), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa08480	NM_169798.3 Latest version!	Drosophila melanogaster 14-3-3epsilon, transcript variant D (14-3-3epsilon), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa08481	NM_169796.2 Latest version!	Drosophila melanogaster 14-3-3epsilon, transcript variant A (14-3-3epsilon), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa08483	NM_169799.2 Latest version!	Drosophila melanogaster 14-3-3epsilon, transcript variant C (14-3-3epsilon), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

14-3-3epsilon ( NM_169797.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732310.1 Drosophila melanogaster 14-3-3epsilon, transcript variant B (14-3-3epsilon), mRNA.

Price & Availability↑

CloneID	OFa08479
Clone ID Related Accession (Same CDS sequence)	NM_169797.3
Accession Version	NM_169797.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	14-3-3epsilon, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169797.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTGAGC GCGAGAACAA TGTGTACAAG GCAAAGCTGG CCGAACAGGC CGAGCGCTAC 
GACGAAATGG TGGAGGCCAT GAAGAAGGTC GCCTCCATGG ACGTAGAGCT GACCGTCGAG 
GAGCGAAATC TGCTGTCGGT GGCGTACAAG AATGTGATTG GAGCACGCCG TGCCTCGTGG 
CGCATCATCA CCTCGATCGA ACAGAAGGAG GAGAACAAGG GGGCCGAGGA GAAATTGGAG 
ATGATCAAAA CCTACCGCGG ACAGGTGGAG AAGGAGCTGC GCGACATCTG CTCGGATATA 
CTGAACGTGC TCGAGAAGCA TCTCATTCCA TGCGCCACAT CCGGCGAAAG CAAAGTATTC 
TACTATAAGA TGAAGGGCGA CTACCATCGC TACCTGGCCG AATTCGCCAC CGGCTCCGAC 
CGCAAGGATG CGGCAGAGAA CTCGCTGATT GCCTACAAGG CGGCCAGCGA TATTGCCATG 
AACGATCTGC CACCAACACA CCCCATCCGT TTGGGCTTGG CATTGAACTT CTCGGTGTTC 
TACTATGAGA TTCTCAACTC GCCGGACCGC GCTTGCCGCT TGGCGAAAGC CGCTTTCGAT 
GATGCCATTG CCGAGTTGGA TACACTGAGC GAAGAGAGCT ACAAAGACTC GACACTCATC 
ATGCAGCTGC TGCGCGACAA CCTCACATTA TGGACGTCCG ATATGCAGGC AGAAGTAGAC 
CCCAACGCAG GTGATGGCGA GCCCAAAGAG CAAATCCAGG ATGTTGAGGA TCAGGACGTG 
TCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732310.1
CDS	316..1101
Translation	MTERENNVYKAKLAEQAERYDEMVEAMKKVASMDVELTVEERNLLSVAYKNVIGARRASWRIITSIEQKEENKGAEEKLEMIKTYRGQVEKELRDICSDILNVLEKHLIPCATSGESKVFYYKMKGDYHRYLAEFATGSDRKDAAENSLIAYKAASDIAMNDLPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQAEVDPNAGDGEPKEQIQDVEDQDVS

Target ORF information:

RefSeq Version	NM_169797.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster 14-3-3epsilon, transcript variant B (14-3-3epsilon), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169797.3

ORF Insert Sequence:

ATGACTGAGC GCGAGAACAA TGTGTACAAG GCAAAGCTGG CCGAACAGGC CGAGCGCTAC 
GACGAAATGG TGGAGGCCAT GAAGAAGGTC GCCTCCATGG ACGTAGAGCT GACCGTCGAG 
GAGCGAAATC TGCTGTCGGT GGCGTACAAG AATGTGATTG GAGCACGCCG TGCCTCGTGG 
CGCATCATCA CCTCGATCGA ACAGAAGGAG GAGAACAAGG GGGCCGAGGA GAAATTGGAG 
ATGATCAAAA CCTACCGCGG ACAGGTGGAG AAGGAGCTGC GCGACATCTG CTCGGATATA 
CTGAACGTGC TCGAGAAGCA TCTCATTCCA TGCGCCACAT CCGGCGAAAG CAAAGTATTC 
TACTATAAGA TGAAGGGCGA CTACCATCGC TACCTGGCCG AATTCGCCAC CGGCTCCGAC 
CGCAAGGATG CGGCAGAGAA CTCGCTGATT GCCTACAAGG CGGCCAGCGA TATTGCCATG 
AACGATCTGC CACCAACACA CCCCATCCGT TTGGGCTTGG CATTGAACTT CTCGGTGTTC 
TACTATGAGA TTCTCAACTC GCCGGACCGC GCTTGCCGCT TGGCGAAAGC CGCTTTCGAT 
GATGCCATTG CCGAGTTGGA TACACTGAGC GAAGAGAGCT ACAAAGACTC GACACTCATC 
ATGCAGCTGC TGCGCGACAA CCTCACATTA TGGACGTCCG ATATGCAGGC AGAAGTAGAC 
CCCAACGCAG GTGATGGCGA GCCCAAAGAG CAAATCCAGG ATGTTGAGGA TCAGGACGTG 
TCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

14-3-3epsilon ( NM_169798.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732311.1 Drosophila melanogaster 14-3-3epsilon, transcript variant D (14-3-3epsilon), mRNA.

Price & Availability↑

CloneID	OFa08480
Clone ID Related Accession (Same CDS sequence)	NM_169798.3
Accession Version	NM_169798.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	14-3-3epsilon, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169798.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTGAGC GCGAGAACAA TGTGTACAAG GCAAAGCTGG CCGAACAGGC CGAGCGCTAC 
GACGAAATGG TGGAGGCCAT GAAGAAGGTC GCCTCCATGG ACGTAGAGCT GACCGTCGAG 
GAGCGAAATC TGCTGTCGGT GGCGTACAAG AATGTGATTG GAGCACGCCG TGCCTCGTGG 
CGCATCATCA CCTCGATCGA ACAGAAGGAG GAGAACAAGG GGGCCGAGGA GAAATTGGAG 
ATGATCAAAA CCTACCGCGG ACAGGTGGAG AAGGAGCTGC GCGACATCTG CTCGGATATA 
CTGAACGTGC TCGAGAAGCA TCTCATTCCA TGCGCCACAT CCGGCGAAAG CAAAGTATTC 
TACTATAAGA TGAAGGGCGA CTACCATCGC TACCTGGCCG AATTCGCCAC CGGCTCCGAC 
CGCAAGGATG CGGCAGAGAA CTCGCTGATT GCCTACAAGG CGGCCAGCGA TATTGCCATG 
AACGATCTGC CACCAACACA CCCCATCCGT TTGGGCTTGG CATTGAACTT CTCGGTGTTC 
TACTATGAGA TTCTCAACTC GCCGGACCGC GCTTGCCGCT TGGCGAAAGC CGCTTTCGAT 
GATGCCATTG CCGAGTTGGA TACACTGAGC GAAGAGAGCT ACAAAGACTC GACACTCATC 
ATGCAGCTGC TGCGCGACAA CCTCACATTA TGGACGTCCG ATATGCAGGC AGAAGACCCC 
AACGCAGGTG ATGGCGAGCC CAAAGAGCAA ATCCAGGATG TTGAGGATCA GGACGTGTCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732311.1
CDS	316..1098
Translation	MTERENNVYKAKLAEQAERYDEMVEAMKKVASMDVELTVEERNLLSVAYKNVIGARRASWRIITSIEQKEENKGAEEKLEMIKTYRGQVEKELRDICSDILNVLEKHLIPCATSGESKVFYYKMKGDYHRYLAEFATGSDRKDAAENSLIAYKAASDIAMNDLPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQAEDPNAGDGEPKEQIQDVEDQDVS

Target ORF information:

RefSeq Version	NM_169798.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster 14-3-3epsilon, transcript variant D (14-3-3epsilon), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169798.3

ORF Insert Sequence:

ATGACTGAGC GCGAGAACAA TGTGTACAAG GCAAAGCTGG CCGAACAGGC CGAGCGCTAC 
GACGAAATGG TGGAGGCCAT GAAGAAGGTC GCCTCCATGG ACGTAGAGCT GACCGTCGAG 
GAGCGAAATC TGCTGTCGGT GGCGTACAAG AATGTGATTG GAGCACGCCG TGCCTCGTGG 
CGCATCATCA CCTCGATCGA ACAGAAGGAG GAGAACAAGG GGGCCGAGGA GAAATTGGAG 
ATGATCAAAA CCTACCGCGG ACAGGTGGAG AAGGAGCTGC GCGACATCTG CTCGGATATA 
CTGAACGTGC TCGAGAAGCA TCTCATTCCA TGCGCCACAT CCGGCGAAAG CAAAGTATTC 
TACTATAAGA TGAAGGGCGA CTACCATCGC TACCTGGCCG AATTCGCCAC CGGCTCCGAC 
CGCAAGGATG CGGCAGAGAA CTCGCTGATT GCCTACAAGG CGGCCAGCGA TATTGCCATG 
AACGATCTGC CACCAACACA CCCCATCCGT TTGGGCTTGG CATTGAACTT CTCGGTGTTC 
TACTATGAGA TTCTCAACTC GCCGGACCGC GCTTGCCGCT TGGCGAAAGC CGCTTTCGAT 
GATGCCATTG CCGAGTTGGA TACACTGAGC GAAGAGAGCT ACAAAGACTC GACACTCATC 
ATGCAGCTGC TGCGCGACAA CCTCACATTA TGGACGTCCG ATATGCAGGC AGAAGACCCC 
AACGCAGGTG ATGGCGAGCC CAAAGAGCAA ATCCAGGATG TTGAGGATCA GGACGTGTCG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

14-3-3epsilon ( NM_169796.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732309.1 Drosophila melanogaster 14-3-3epsilon, transcript variant A (14-3-3epsilon), mRNA.

Price & Availability↑

CloneID	OFa08481
Clone ID Related Accession (Same CDS sequence)	NM_169796.2
Accession Version	NM_169796.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	14-3-3epsilon, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_169796.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTGAGC GCGAGAACAA TGTGTACAAG GCAAAGCTGG CCGAACAGGC CGAGCGCTAC 
GACGAAATGG TGGAGGCCAT GAAGAAGGTC GCCTCCATGG ACGTAGAGCT GACCGTCGAG 
GAGCGAAATC TGCTGTCGGT GGCGTACAAG AATGTGATTG GAGCACGCCG TGCCTCGTGG 
CGCATCATCA CCTCGATCGA ACAGAAGGAG GAGAACAAGG GGGCCGAGGA GAAATTGGAG 
ATGATCAAAA CCTACCGCGG ACAGGTGGAG AAGGAGCTGC GCGACATCTG CTCGGATATA 
CTGAACGTGC TCGAGAAGCA TCTCATTCCA TGCGCCACAT CCGGCGAAAG CAAAGTATTC 
TACTATAAGA TGAAGGGCGA CTACCATCGC TACCTGGCCG AATTCGCCAC CGGCTCCGAC 
CGCAAGGATG CGGCAGAGAA CTCGCTGATT GCCTACAAGG CGGCCAGCGA TATTGCCATG 
AACGATCTGC CACCAACACA CCCCATCCGT TTGGGCTTGG CATTGAACTT CTCGGTGTTC 
TACTATGAGA TTCTCAACTC GCCGGACCGC GCTTGCCGCT TGGCGAAAGC CGCTTTCGAT 
GATGCCATTG CCGAGTTGGA TACACTGAGC GAAGAGAGCT ACAAAGACTC GACACTCATC 
ATGCAGCTGC TGCGCGACAA CCTCACATTA TGGACGTCCG ATATGCAGGC AGAAGAAGTA 
GACCCCAACG CAGGTGATGG CGAGCCCAAA GAGCAAATCC AGGATGTTGA GGATCAGGAC 
GTGTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732309.1
CDS	160..948
Translation	MTERENNVYKAKLAEQAERYDEMVEAMKKVASMDVELTVEERNLLSVAYKNVIGARRASWRIITSIEQKEENKGAEEKLEMIKTYRGQVEKELRDICSDILNVLEKHLIPCATSGESKVFYYKMKGDYHRYLAEFATGSDRKDAAENSLIAYKAASDIAMNDLPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQAEEVDPNAGDGEPKEQIQDVEDQDVS

Target ORF information:

RefSeq Version	NM_169796.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster 14-3-3epsilon, transcript variant A (14-3-3epsilon), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169796.2

ORF Insert Sequence:

ATGACTGAGC GCGAGAACAA TGTGTACAAG GCAAAGCTGG CCGAACAGGC CGAGCGCTAC 
GACGAAATGG TGGAGGCCAT GAAGAAGGTC GCCTCCATGG ACGTAGAGCT GACCGTCGAG 
GAGCGAAATC TGCTGTCGGT GGCGTACAAG AATGTGATTG GAGCACGCCG TGCCTCGTGG 
CGCATCATCA CCTCGATCGA ACAGAAGGAG GAGAACAAGG GGGCCGAGGA GAAATTGGAG 
ATGATCAAAA CCTACCGCGG ACAGGTGGAG AAGGAGCTGC GCGACATCTG CTCGGATATA 
CTGAACGTGC TCGAGAAGCA TCTCATTCCA TGCGCCACAT CCGGCGAAAG CAAAGTATTC 
TACTATAAGA TGAAGGGCGA CTACCATCGC TACCTGGCCG AATTCGCCAC CGGCTCCGAC 
CGCAAGGATG CGGCAGAGAA CTCGCTGATT GCCTACAAGG CGGCCAGCGA TATTGCCATG 
AACGATCTGC CACCAACACA CCCCATCCGT TTGGGCTTGG CATTGAACTT CTCGGTGTTC 
TACTATGAGA TTCTCAACTC GCCGGACCGC GCTTGCCGCT TGGCGAAAGC CGCTTTCGAT 
GATGCCATTG CCGAGTTGGA TACACTGAGC GAAGAGAGCT ACAAAGACTC GACACTCATC 
ATGCAGCTGC TGCGCGACAA CCTCACATTA TGGACGTCCG ATATGCAGGC AGAAGAAGTA 
GACCCCAACG CAGGTGATGG CGAGCCCAAA GAGCAAATCC AGGATGTTGA GGATCAGGAC 
GTGTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

14-3-3epsilon ( NM_169799.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732312.1 Drosophila melanogaster 14-3-3epsilon, transcript variant C (14-3-3epsilon), mRNA.

Price & Availability↑

CloneID	OFa08483
Clone ID Related Accession (Same CDS sequence)	NM_169799.2
Accession Version	NM_169799.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	14-3-3epsilon, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_169799.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTGAGC GCGAGAACAA TGTGTACAAG GCAAAGCTGG CCGAACAGGC CGAGCGCTAC 
GACGAAATGG TGGAGGCCAT GAAGAAGGTC GCCTCCATGG ACGTAGAGCT GACCGTCGAG 
GAGCGAAATC TGCTGTCGGT GGCGTACAAG AATGTGATTG GAGCACGCCG TGCCTCGTGG 
CGCATCATCA CCTCGATCGA ACAGAAGGAG GAGAACAAGG GGGCCGAGGA GAAATTGGAG 
ATGATCAAAA CCTACCGCGG ACAGGTGGAG AAGGAGCTGC GCGACATCTG CTCGGATATA 
CTGAACGTGC TCGAGAAGCA TCTCATTCCA TGCGCCACAT CCGGCGAAAG CAAAGTATTC 
TACTATAAGA TGAAGGGCGA CTACCATCGC TACCTGGCCG AATTCGCCAC CGGCTCCGAC 
CGCAAGGATG CGGCAGAGAA CTCGCTGATT GCCTACAAGG CGGCCAGCGA TATTGCCATG 
AACGATCTGC CACCAACACA CCCCATCCGT TTGGGCTTGG CATTGAACTT CTCGGTGTTC 
TACTATGAGA TTCTCAACTC GCCGGACCGC GCTTGCCGCT TGGCGAAAGC CGCTTTCGAT 
GATGCCATTG CCGAGTTGGA TACACTGAGC GAAGAGAGCT ACAAAGACTC GACACTCATC 
ATGCAGCTGC TGCGCGACAA CCTCACATTA TGGACGTCCG ATATGCAGGC AGAAGGTGAT 
GGCGAGCCCA AAGAGCAAAT CCAGGATGTT GAGGATCAGG ACGTGTCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732312.1
CDS	160..930
Translation	MTERENNVYKAKLAEQAERYDEMVEAMKKVASMDVELTVEERNLLSVAYKNVIGARRASWRIITSIEQKEENKGAEEKLEMIKTYRGQVEKELRDICSDILNVLEKHLIPCATSGESKVFYYKMKGDYHRYLAEFATGSDRKDAAENSLIAYKAASDIAMNDLPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQAEGDGEPKEQIQDVEDQDVS

Target ORF information:

RefSeq Version	NM_169799.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster 14-3-3epsilon, transcript variant C (14-3-3epsilon), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169799.2

ORF Insert Sequence:

ATGACTGAGC GCGAGAACAA TGTGTACAAG GCAAAGCTGG CCGAACAGGC CGAGCGCTAC 
GACGAAATGG TGGAGGCCAT GAAGAAGGTC GCCTCCATGG ACGTAGAGCT GACCGTCGAG 
GAGCGAAATC TGCTGTCGGT GGCGTACAAG AATGTGATTG GAGCACGCCG TGCCTCGTGG 
CGCATCATCA CCTCGATCGA ACAGAAGGAG GAGAACAAGG GGGCCGAGGA GAAATTGGAG 
ATGATCAAAA CCTACCGCGG ACAGGTGGAG AAGGAGCTGC GCGACATCTG CTCGGATATA 
CTGAACGTGC TCGAGAAGCA TCTCATTCCA TGCGCCACAT CCGGCGAAAG CAAAGTATTC 
TACTATAAGA TGAAGGGCGA CTACCATCGC TACCTGGCCG AATTCGCCAC CGGCTCCGAC 
CGCAAGGATG CGGCAGAGAA CTCGCTGATT GCCTACAAGG CGGCCAGCGA TATTGCCATG 
AACGATCTGC CACCAACACA CCCCATCCGT TTGGGCTTGG CATTGAACTT CTCGGTGTTC 
TACTATGAGA TTCTCAACTC GCCGGACCGC GCTTGCCGCT TGGCGAAAGC CGCTTTCGAT 
GATGCCATTG CCGAGTTGGA TACACTGAGC GAAGAGAGCT ACAAAGACTC GACACTCATC 
ATGCAGCTGC TGCGCGACAA CCTCACATTA TGGACGTCCG ATATGCAGGC AGAAGGTGAT 
GGCGAGCCCA AAGAGCAAAT CCAGGATGTT GAGGATCAGG ACGTGTCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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14-3-3epsilon cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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