kuk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	kuk
Entrez Gene ID	42063
Full Name	kugelkern
Synonyms	CG5175,Dmel\CG5175,Kuk,char,kuk/char
General protein information	Preferred Names kugelkern Names CG5175 gene product from transcript CG5175-RA CG5175-PA CG5175-PB CG5175-PC Kugelkern/Charleston charleston kugelkem kuk-PA kuk-PB kuk-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 89E12-89E12

mRNA and Protein(s)

mRNA	Protein	Name
NM_001260219.1	NP_001247148.1	kugelkern, isoform C
NM_142338.3	NP_650595.1	kugelkern, isoform A
NM_169745.3	NP_732204.1	kugelkern, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Kugelkern and lamin Dm0 affect nuclear shape without requiring filament formation or the presence of a classical nuclear lamina.
Title: Farnesylated nuclear proteins Kugelkern and lamin Dm0 affect nuclear morphology by directly interacting with the nuclear membrane.

Induced expression of the farnesylated lamina proteins Lamin B and Kugelkern cause aberrant nuclear shapes and reduce the lifespan of adult flies.
Title: The farnesylated nuclear proteins KUGELKERN and LAMIN B promote aging-like phenotypes in Drosophila flies.

Kugelkern encodes a coiled-coil protein with nuclear localization signal and CxxM motif and is required for nuclear elongation.
Title: Developmental control of nuclear size and shape by Kugelkern and Kurzkern.

Char is lipid anchored at the nuclear envelope by a farnesylation group, and localises at the inner nuclear membrane. Char forms a scaffold that regulates nuclear architecture to constrain nuclei in tight columnar epithelial cells.
Title: Developmental control of nuclear morphogenesis and anchoring by charleston, identified in a functional genomic screen of Drosophila cellularisation.

The following kuk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kuk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05192	NM_169745.3 Latest version!	Drosophila melanogaster kugelkern (kuk), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa05192	NM_001260219.1 Latest version!	Drosophila melanogaster kugelkern (kuk), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa05192	NM_142338.3 Latest version!	Drosophila melanogaster kugelkern (kuk), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kuk ( NM_001260219.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247148.1 Drosophila melanogaster kugelkern (kuk), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa05192
Clone ID Related Accession (Same CDS sequence)	NM_001260219.1 , NM_142338.3 , NM_169745.3
Accession Version	NM_001260219.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kugelkern, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTAAGCA AAATGGCTAG CAACACAAGC GGCGGGGGAG GTACCACTTC AGGCACTGGA 
ACGGGACGTC GTCTGCCACT CTCGCTGAGC GGCGGTTCCA CCGTCACGCG CTTGCAGCAA 
TCTTCTGGCA GCAACGGCCG CTCAAATAGC CCCCAAAATG GGAACAAAAA GATCACAGAG 
TCATTTTTGC CGGTAAAAAA GAGCAACCAA CCGCGGACCA CTTCGACTGG ATCCACCGGA 
TCCAGCCAGA ACGGCGTAAG CAAGCCCACA AATGGCAAAG ATAAGTCACA GAATGGCAGC 
TCAACAGGCA AAGACCAGAA GGTAGAGAAA TCTAAAGATA AGTTGCCGGA AAAGAAGGCA 
AAGGAAGTGG GTGCCGAGAA TAAGGATAAG GATGGCGCTG CCGAGATGAC AGATATGGAG 
GTTGTGGTCG AAAATAAGCA AAAGGAGGTG GTATCCGAAA ATAAGCAAAA TGAGGTGGTA 
TCTGAAAATA AGGAAGTGGT ATCTGAAAAT AAGGAGGTGG TATCCGAAAA TAAGGAGGTG 
GCTTCCGAAA AAAAGGTGGT GGTAGCTGAA GTTCCCTCCG ACAAAGACGA CAACGGAGAG 
ACCAAAAAAG ATGATGCTAC CGTTGAGAGT ACTGCTCCTG CTTCTCAAAC GCCTGCGGAT 
GCTGCGGTAA TCATCGATGA AGTAGTGTTT ATTCCCGATC TCAGCACTCC TACTAAGGAT 
CGCAAGTCTA CACGCAAGTC GGGTAGCCAG CAAAAGTCAC CCATCAGCAA GGCAAGCAAC 
GCTTCGCCTG TCCAGAAGAT AAAAACCGAA GCAGTTTCTT CATCGACATC GGGCTCTTTG 
ACTCCACAAG AAGATGTCGA TATGGAACCG CTACAAGTGG ATGCCTCTCC CATACGCAGC 
GTCCACGGCA ATTTGAATGC CGTGCCGACG GCTACATCCA CGCCCGGACG CAGTCTCTTC 
AGTTTCCGTG GTAGCAACAA GCAAAGCACC GAGGTAGAGC TGGCTGCCCA GACTTCCAGC 
TCGCCGGCAG GAACTCCGGC TCGCACTTTC GCCCAGATCA GCGGCAGGCG TAGCATACGA 
CCCATCGACT CACTGACGCC CTCGAAAATA GGCACCTACA GGTGCGGGAA CAGCGATCTG 
GACACCTCGA ACTGCACGAA CGCAAGCATG AATGCCACAG TCGGATCAGA GATCCCCAAC 
AGCTCGTCAT TTTCATTTTC ATTCTTTGGC CGTGGCCGCA AGCGTGAACG CACTCCCCCA 
CCACTGACGG CCTCTCAGTC GACCAGTGAC CTGGGCCACG ACGTGGATAT GTCGCCGCCA 
AAGCGCGCCC GCTTCGACTT GTTCAGCCTG AATTTGGCTT CTCCCTTTAG CTTGCTGCGC 
TCGAGATTCT CCAAGGCAAC AATCGGTACT CCGACCCGTC TGCGTCTGGA GCCGAACAGT 
TCCGGCGTGG AGGAGCAACA TCTGAACACA TCTACGACTG GCGTACCAGA AGAGGTTGAT 
GTGGAGGAGG AACAACCGGC CGAAGACGTG ACCGTTGGTC AGGAGGCCGG AGCGGAGGAA 
ATCCCCGCCA AGAAGGATGT CGAGGATAAG AACGTCAATG GCCAGGATGG CGACAAGGAG 
TCCAGCGATG GAGAAAACAT TGACCCTGTT GTAGAGGTGG CTGATGTTGC CGCGGTCGCT 
GCCGAGGGCC GATCCATCTG CTCAATTATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247148.1
CDS	116..1828
Translation	MLSKMASNTSGGGGTTSGTGTGRRLPLSLSGGSTVTRLQQSSGSNGRSNSPQNGNKKITESFLPVKKSNQPRTTSTGSTGSSQNGVSKPTNGKDKSQNGSSTGKDQKVEKSKDKLPEKKAKEVGAENKDKDGAAEMTDMEVVVENKQKEVVSENKQNEVVSENKEVVSENKEVVSENKEVASEKKVVVAEVPSDKDDNGETKKDDATVESTAPASQTPADAAVIIDEVVFIPDLSTPTKDRKSTRKSGSQQKSPISKASNASPVQKIKTEAVSSSTSGSLTPQEDVDMEPLQVDASPIRSVHGNLNAVPTATSTPGRSLFSFRGSNKQSTEVELAAQTSSSPAGTPARTFAQISGRRSIRPIDSLTPSKIGTYRCGNSDLDTSNCTNASMNATVGSEIPNSSSFSFSFFGRGRKRERTPPPLTASQSTSDLGHDVDMSPPKRARFDLFSLNLASPFSLLRSRFSKATIGTPTRLRLEPNSSGVEEQHLNTSTTGVPEEVDVEEEQPAEDVTVGQEAGAEEIPAKKDVEDKNVNGQDGDKESSDGENIDPVVEVADVAAVAAEGRSICSIM

Target ORF information:

RefSeq Version	NM_001260219.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kugelkern (kuk), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260219.1

ORF Insert Sequence:

ATGTTAAGCA AAATGGCTAG CAACACAAGC GGCGGGGGAG GTACCACTTC AGGCACTGGA 
ACGGGACGTC GTCTGCCACT CTCGCTGAGC GGCGGTTCCA CCGTCACGCG CTTGCAGCAA 
TCTTCTGGCA GCAACGGCCG CTCAAATAGC CCCCAAAATG GGAACAAAAA GATCACAGAG 
TCATTTTTGC CGGTAAAAAA GAGCAACCAA CCGCGGACCA CTTCGACTGG ATCCACCGGA 
TCCAGCCAGA ACGGCGTAAG CAAGCCCACA AATGGCAAAG ATAAGTCACA GAATGGCAGC 
TCAACAGGCA AAGACCAGAA GGTAGAGAAA TCTAAAGATA AGTTGCCGGA AAAGAAGGCA 
AAGGAAGTGG GTGCCGAGAA TAAGGATAAG GATGGCGCTG CCGAGATGAC AGATATGGAG 
GTTGTGGTCG AAAATAAGCA AAAGGAGGTG GTATCCGAAA ATAAGCAAAA TGAGGTGGTA 
TCTGAAAATA AGGAAGTGGT ATCTGAAAAT AAGGAGGTGG TATCCGAAAA TAAGGAGGTG 
GCTTCCGAAA AAAAGGTGGT GGTAGCTGAA GTTCCCTCCG ACAAAGACGA CAACGGAGAG 
ACCAAAAAAG ATGATGCTAC CGTTGAGAGT ACTGCTCCTG CTTCTCAAAC GCCTGCGGAT 
GCTGCGGTAA TCATCGATGA AGTAGTGTTT ATTCCCGATC TCAGCACTCC TACTAAGGAT 
CGCAAGTCTA CACGCAAGTC GGGTAGCCAG CAAAAGTCAC CCATCAGCAA GGCAAGCAAC 
GCTTCGCCTG TCCAGAAGAT AAAAACCGAA GCAGTTTCTT CATCGACATC GGGCTCTTTG 
ACTCCACAAG AAGATGTCGA TATGGAACCG CTACAAGTGG ATGCCTCTCC CATACGCAGC 
GTCCACGGCA ATTTGAATGC CGTGCCGACG GCTACATCCA CGCCCGGACG CAGTCTCTTC 
AGTTTCCGTG GTAGCAACAA GCAAAGCACC GAGGTAGAGC TGGCTGCCCA GACTTCCAGC 
TCGCCGGCAG GAACTCCGGC TCGCACTTTC GCCCAGATCA GCGGCAGGCG TAGCATACGA 
CCCATCGACT CACTGACGCC CTCGAAAATA GGCACCTACA GGTGCGGGAA CAGCGATCTG 
GACACCTCGA ACTGCACGAA CGCAAGCATG AATGCCACAG TCGGATCAGA GATCCCCAAC 
AGCTCGTCAT TTTCATTTTC ATTCTTTGGC CGTGGCCGCA AGCGTGAACG CACTCCCCCA 
CCACTGACGG CCTCTCAGTC GACCAGTGAC CTGGGCCACG ACGTGGATAT GTCGCCGCCA 
AAGCGCGCCC GCTTCGACTT GTTCAGCCTG AATTTGGCTT CTCCCTTTAG CTTGCTGCGC 
TCGAGATTCT CCAAGGCAAC AATCGGTACT CCGACCCGTC TGCGTCTGGA GCCGAACAGT 
TCCGGCGTGG AGGAGCAACA TCTGAACACA TCTACGACTG GCGTACCAGA AGAGGTTGAT 
GTGGAGGAGG AACAACCGGC CGAAGACGTG ACCGTTGGTC AGGAGGCCGG AGCGGAGGAA 
ATCCCCGCCA AGAAGGATGT CGAGGATAAG AACGTCAATG GCCAGGATGG CGACAAGGAG 
TCCAGCGATG GAGAAAACAT TGACCCTGTT GTAGAGGTGG CTGATGTTGC CGCGGTCGCT 
GCCGAGGGCC GATCCATCTG CTCAATTATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kuk ( NM_142338.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650595.1 Drosophila melanogaster kugelkern (kuk), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa05192
Clone ID Related Accession (Same CDS sequence)	NM_001260219.1 , NM_142338.3 , NM_169745.3
Accession Version	NM_142338.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kugelkern, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_142338.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTAAGCA AAATGGCTAG CAACACAAGC GGCGGGGGAG GTACCACTTC AGGCACTGGA 
ACGGGACGTC GTCTGCCACT CTCGCTGAGC GGCGGTTCCA CCGTCACGCG CTTGCAGCAA 
TCTTCTGGCA GCAACGGCCG CTCAAATAGC CCCCAAAATG GGAACAAAAA GATCACAGAG 
TCATTTTTGC CGGTAAAAAA GAGCAACCAA CCGCGGACCA CTTCGACTGG ATCCACCGGA 
TCCAGCCAGA ACGGCGTAAG CAAGCCCACA AATGGCAAAG ATAAGTCACA GAATGGCAGC 
TCAACAGGCA AAGACCAGAA GGTAGAGAAA TCTAAAGATA AGTTGCCGGA AAAGAAGGCA 
AAGGAAGTGG GTGCCGAGAA TAAGGATAAG GATGGCGCTG CCGAGATGAC AGATATGGAG 
GTTGTGGTCG AAAATAAGCA AAAGGAGGTG GTATCCGAAA ATAAGCAAAA TGAGGTGGTA 
TCTGAAAATA AGGAAGTGGT ATCTGAAAAT AAGGAGGTGG TATCCGAAAA TAAGGAGGTG 
GCTTCCGAAA AAAAGGTGGT GGTAGCTGAA GTTCCCTCCG ACAAAGACGA CAACGGAGAG 
ACCAAAAAAG ATGATGCTAC CGTTGAGAGT ACTGCTCCTG CTTCTCAAAC GCCTGCGGAT 
GCTGCGGTAA TCATCGATGA AGTAGTGTTT ATTCCCGATC TCAGCACTCC TACTAAGGAT 
CGCAAGTCTA CACGCAAGTC GGGTAGCCAG CAAAAGTCAC CCATCAGCAA GGCAAGCAAC 
GCTTCGCCTG TCCAGAAGAT AAAAACCGAA GCAGTTTCTT CATCGACATC GGGCTCTTTG 
ACTCCACAAG AAGATGTCGA TATGGAACCG CTACAAGTGG ATGCCTCTCC CATACGCAGC 
GTCCACGGCA ATTTGAATGC CGTGCCGACG GCTACATCCA CGCCCGGACG CAGTCTCTTC 
AGTTTCCGTG GTAGCAACAA GCAAAGCACC GAGGTAGAGC TGGCTGCCCA GACTTCCAGC 
TCGCCGGCAG GAACTCCGGC TCGCACTTTC GCCCAGATCA GCGGCAGGCG TAGCATACGA 
CCCATCGACT CACTGACGCC CTCGAAAATA GGCACCTACA GGTGCGGGAA CAGCGATCTG 
GACACCTCGA ACTGCACGAA CGCAAGCATG AATGCCACAG TCGGATCAGA GATCCCCAAC 
AGCTCGTCAT TTTCATTTTC ATTCTTTGGC CGTGGCCGCA AGCGTGAACG CACTCCCCCA 
CCACTGACGG CCTCTCAGTC GACCAGTGAC CTGGGCCACG ACGTGGATAT GTCGCCGCCA 
AAGCGCGCCC GCTTCGACTT GTTCAGCCTG AATTTGGCTT CTCCCTTTAG CTTGCTGCGC 
TCGAGATTCT CCAAGGCAAC AATCGGTACT CCGACCCGTC TGCGTCTGGA GCCGAACAGT 
TCCGGCGTGG AGGAGCAACA TCTGAACACA TCTACGACTG GCGTACCAGA AGAGGTTGAT 
GTGGAGGAGG AACAACCGGC CGAAGACGTG ACCGTTGGTC AGGAGGCCGG AGCGGAGGAA 
ATCCCCGCCA AGAAGGATGT CGAGGATAAG AACGTCAATG GCCAGGATGG CGACAAGGAG 
TCCAGCGATG GAGAAAACAT TGACCCTGTT GTAGAGGTGG CTGATGTTGC CGCGGTCGCT 
GCCGAGGGCC GATCCATCTG CTCAATTATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650595.1
CDS	147..1859
Translation	MLSKMASNTSGGGGTTSGTGTGRRLPLSLSGGSTVTRLQQSSGSNGRSNSPQNGNKKITESFLPVKKSNQPRTTSTGSTGSSQNGVSKPTNGKDKSQNGSSTGKDQKVEKSKDKLPEKKAKEVGAENKDKDGAAEMTDMEVVVENKQKEVVSENKQNEVVSENKEVVSENKEVVSENKEVASEKKVVVAEVPSDKDDNGETKKDDATVESTAPASQTPADAAVIIDEVVFIPDLSTPTKDRKSTRKSGSQQKSPISKASNASPVQKIKTEAVSSSTSGSLTPQEDVDMEPLQVDASPIRSVHGNLNAVPTATSTPGRSLFSFRGSNKQSTEVELAAQTSSSPAGTPARTFAQISGRRSIRPIDSLTPSKIGTYRCGNSDLDTSNCTNASMNATVGSEIPNSSSFSFSFFGRGRKRERTPPPLTASQSTSDLGHDVDMSPPKRARFDLFSLNLASPFSLLRSRFSKATIGTPTRLRLEPNSSGVEEQHLNTSTTGVPEEVDVEEEQPAEDVTVGQEAGAEEIPAKKDVEDKNVNGQDGDKESSDGENIDPVVEVADVAAVAAEGRSICSIM

Target ORF information:

RefSeq Version	NM_142338.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kugelkern (kuk), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142338.3

ORF Insert Sequence:

ATGTTAAGCA AAATGGCTAG CAACACAAGC GGCGGGGGAG GTACCACTTC AGGCACTGGA 
ACGGGACGTC GTCTGCCACT CTCGCTGAGC GGCGGTTCCA CCGTCACGCG CTTGCAGCAA 
TCTTCTGGCA GCAACGGCCG CTCAAATAGC CCCCAAAATG GGAACAAAAA GATCACAGAG 
TCATTTTTGC CGGTAAAAAA GAGCAACCAA CCGCGGACCA CTTCGACTGG ATCCACCGGA 
TCCAGCCAGA ACGGCGTAAG CAAGCCCACA AATGGCAAAG ATAAGTCACA GAATGGCAGC 
TCAACAGGCA AAGACCAGAA GGTAGAGAAA TCTAAAGATA AGTTGCCGGA AAAGAAGGCA 
AAGGAAGTGG GTGCCGAGAA TAAGGATAAG GATGGCGCTG CCGAGATGAC AGATATGGAG 
GTTGTGGTCG AAAATAAGCA AAAGGAGGTG GTATCCGAAA ATAAGCAAAA TGAGGTGGTA 
TCTGAAAATA AGGAAGTGGT ATCTGAAAAT AAGGAGGTGG TATCCGAAAA TAAGGAGGTG 
GCTTCCGAAA AAAAGGTGGT GGTAGCTGAA GTTCCCTCCG ACAAAGACGA CAACGGAGAG 
ACCAAAAAAG ATGATGCTAC CGTTGAGAGT ACTGCTCCTG CTTCTCAAAC GCCTGCGGAT 
GCTGCGGTAA TCATCGATGA AGTAGTGTTT ATTCCCGATC TCAGCACTCC TACTAAGGAT 
CGCAAGTCTA CACGCAAGTC GGGTAGCCAG CAAAAGTCAC CCATCAGCAA GGCAAGCAAC 
GCTTCGCCTG TCCAGAAGAT AAAAACCGAA GCAGTTTCTT CATCGACATC GGGCTCTTTG 
ACTCCACAAG AAGATGTCGA TATGGAACCG CTACAAGTGG ATGCCTCTCC CATACGCAGC 
GTCCACGGCA ATTTGAATGC CGTGCCGACG GCTACATCCA CGCCCGGACG CAGTCTCTTC 
AGTTTCCGTG GTAGCAACAA GCAAAGCACC GAGGTAGAGC TGGCTGCCCA GACTTCCAGC 
TCGCCGGCAG GAACTCCGGC TCGCACTTTC GCCCAGATCA GCGGCAGGCG TAGCATACGA 
CCCATCGACT CACTGACGCC CTCGAAAATA GGCACCTACA GGTGCGGGAA CAGCGATCTG 
GACACCTCGA ACTGCACGAA CGCAAGCATG AATGCCACAG TCGGATCAGA GATCCCCAAC 
AGCTCGTCAT TTTCATTTTC ATTCTTTGGC CGTGGCCGCA AGCGTGAACG CACTCCCCCA 
CCACTGACGG CCTCTCAGTC GACCAGTGAC CTGGGCCACG ACGTGGATAT GTCGCCGCCA 
AAGCGCGCCC GCTTCGACTT GTTCAGCCTG AATTTGGCTT CTCCCTTTAG CTTGCTGCGC 
TCGAGATTCT CCAAGGCAAC AATCGGTACT CCGACCCGTC TGCGTCTGGA GCCGAACAGT 
TCCGGCGTGG AGGAGCAACA TCTGAACACA TCTACGACTG GCGTACCAGA AGAGGTTGAT 
GTGGAGGAGG AACAACCGGC CGAAGACGTG ACCGTTGGTC AGGAGGCCGG AGCGGAGGAA 
ATCCCCGCCA AGAAGGATGT CGAGGATAAG AACGTCAATG GCCAGGATGG CGACAAGGAG 
TCCAGCGATG GAGAAAACAT TGACCCTGTT GTAGAGGTGG CTGATGTTGC CGCGGTCGCT 
GCCGAGGGCC GATCCATCTG CTCAATTATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kuk ( NM_169745.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732204.1 Drosophila melanogaster kugelkern (kuk), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa05192
Clone ID Related Accession (Same CDS sequence)	NM_001260219.1 , NM_142338.3 , NM_169745.3
Accession Version	NM_169745.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kugelkern, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169745.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTAAGCA AAATGGCTAG CAACACAAGC GGCGGGGGAG GTACCACTTC AGGCACTGGA 
ACGGGACGTC GTCTGCCACT CTCGCTGAGC GGCGGTTCCA CCGTCACGCG CTTGCAGCAA 
TCTTCTGGCA GCAACGGCCG CTCAAATAGC CCCCAAAATG GGAACAAAAA GATCACAGAG 
TCATTTTTGC CGGTAAAAAA GAGCAACCAA CCGCGGACCA CTTCGACTGG ATCCACCGGA 
TCCAGCCAGA ACGGCGTAAG CAAGCCCACA AATGGCAAAG ATAAGTCACA GAATGGCAGC 
TCAACAGGCA AAGACCAGAA GGTAGAGAAA TCTAAAGATA AGTTGCCGGA AAAGAAGGCA 
AAGGAAGTGG GTGCCGAGAA TAAGGATAAG GATGGCGCTG CCGAGATGAC AGATATGGAG 
GTTGTGGTCG AAAATAAGCA AAAGGAGGTG GTATCCGAAA ATAAGCAAAA TGAGGTGGTA 
TCTGAAAATA AGGAAGTGGT ATCTGAAAAT AAGGAGGTGG TATCCGAAAA TAAGGAGGTG 
GCTTCCGAAA AAAAGGTGGT GGTAGCTGAA GTTCCCTCCG ACAAAGACGA CAACGGAGAG 
ACCAAAAAAG ATGATGCTAC CGTTGAGAGT ACTGCTCCTG CTTCTCAAAC GCCTGCGGAT 
GCTGCGGTAA TCATCGATGA AGTAGTGTTT ATTCCCGATC TCAGCACTCC TACTAAGGAT 
CGCAAGTCTA CACGCAAGTC GGGTAGCCAG CAAAAGTCAC CCATCAGCAA GGCAAGCAAC 
GCTTCGCCTG TCCAGAAGAT AAAAACCGAA GCAGTTTCTT CATCGACATC GGGCTCTTTG 
ACTCCACAAG AAGATGTCGA TATGGAACCG CTACAAGTGG ATGCCTCTCC CATACGCAGC 
GTCCACGGCA ATTTGAATGC CGTGCCGACG GCTACATCCA CGCCCGGACG CAGTCTCTTC 
AGTTTCCGTG GTAGCAACAA GCAAAGCACC GAGGTAGAGC TGGCTGCCCA GACTTCCAGC 
TCGCCGGCAG GAACTCCGGC TCGCACTTTC GCCCAGATCA GCGGCAGGCG TAGCATACGA 
CCCATCGACT CACTGACGCC CTCGAAAATA GGCACCTACA GGTGCGGGAA CAGCGATCTG 
GACACCTCGA ACTGCACGAA CGCAAGCATG AATGCCACAG TCGGATCAGA GATCCCCAAC 
AGCTCGTCAT TTTCATTTTC ATTCTTTGGC CGTGGCCGCA AGCGTGAACG CACTCCCCCA 
CCACTGACGG CCTCTCAGTC GACCAGTGAC CTGGGCCACG ACGTGGATAT GTCGCCGCCA 
AAGCGCGCCC GCTTCGACTT GTTCAGCCTG AATTTGGCTT CTCCCTTTAG CTTGCTGCGC 
TCGAGATTCT CCAAGGCAAC AATCGGTACT CCGACCCGTC TGCGTCTGGA GCCGAACAGT 
TCCGGCGTGG AGGAGCAACA TCTGAACACA TCTACGACTG GCGTACCAGA AGAGGTTGAT 
GTGGAGGAGG AACAACCGGC CGAAGACGTG ACCGTTGGTC AGGAGGCCGG AGCGGAGGAA 
ATCCCCGCCA AGAAGGATGT CGAGGATAAG AACGTCAATG GCCAGGATGG CGACAAGGAG 
TCCAGCGATG GAGAAAACAT TGACCCTGTT GTAGAGGTGG CTGATGTTGC CGCGGTCGCT 
GCCGAGGGCC GATCCATCTG CTCAATTATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732204.1
CDS	154..1866
Translation	MLSKMASNTSGGGGTTSGTGTGRRLPLSLSGGSTVTRLQQSSGSNGRSNSPQNGNKKITESFLPVKKSNQPRTTSTGSTGSSQNGVSKPTNGKDKSQNGSSTGKDQKVEKSKDKLPEKKAKEVGAENKDKDGAAEMTDMEVVVENKQKEVVSENKQNEVVSENKEVVSENKEVVSENKEVASEKKVVVAEVPSDKDDNGETKKDDATVESTAPASQTPADAAVIIDEVVFIPDLSTPTKDRKSTRKSGSQQKSPISKASNASPVQKIKTEAVSSSTSGSLTPQEDVDMEPLQVDASPIRSVHGNLNAVPTATSTPGRSLFSFRGSNKQSTEVELAAQTSSSPAGTPARTFAQISGRRSIRPIDSLTPSKIGTYRCGNSDLDTSNCTNASMNATVGSEIPNSSSFSFSFFGRGRKRERTPPPLTASQSTSDLGHDVDMSPPKRARFDLFSLNLASPFSLLRSRFSKATIGTPTRLRLEPNSSGVEEQHLNTSTTGVPEEVDVEEEQPAEDVTVGQEAGAEEIPAKKDVEDKNVNGQDGDKESSDGENIDPVVEVADVAAVAAEGRSICSIM

Target ORF information:

RefSeq Version	NM_169745.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kugelkern (kuk), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169745.3

ORF Insert Sequence:

ATGTTAAGCA AAATGGCTAG CAACACAAGC GGCGGGGGAG GTACCACTTC AGGCACTGGA 
ACGGGACGTC GTCTGCCACT CTCGCTGAGC GGCGGTTCCA CCGTCACGCG CTTGCAGCAA 
TCTTCTGGCA GCAACGGCCG CTCAAATAGC CCCCAAAATG GGAACAAAAA GATCACAGAG 
TCATTTTTGC CGGTAAAAAA GAGCAACCAA CCGCGGACCA CTTCGACTGG ATCCACCGGA 
TCCAGCCAGA ACGGCGTAAG CAAGCCCACA AATGGCAAAG ATAAGTCACA GAATGGCAGC 
TCAACAGGCA AAGACCAGAA GGTAGAGAAA TCTAAAGATA AGTTGCCGGA AAAGAAGGCA 
AAGGAAGTGG GTGCCGAGAA TAAGGATAAG GATGGCGCTG CCGAGATGAC AGATATGGAG 
GTTGTGGTCG AAAATAAGCA AAAGGAGGTG GTATCCGAAA ATAAGCAAAA TGAGGTGGTA 
TCTGAAAATA AGGAAGTGGT ATCTGAAAAT AAGGAGGTGG TATCCGAAAA TAAGGAGGTG 
GCTTCCGAAA AAAAGGTGGT GGTAGCTGAA GTTCCCTCCG ACAAAGACGA CAACGGAGAG 
ACCAAAAAAG ATGATGCTAC CGTTGAGAGT ACTGCTCCTG CTTCTCAAAC GCCTGCGGAT 
GCTGCGGTAA TCATCGATGA AGTAGTGTTT ATTCCCGATC TCAGCACTCC TACTAAGGAT 
CGCAAGTCTA CACGCAAGTC GGGTAGCCAG CAAAAGTCAC CCATCAGCAA GGCAAGCAAC 
GCTTCGCCTG TCCAGAAGAT AAAAACCGAA GCAGTTTCTT CATCGACATC GGGCTCTTTG 
ACTCCACAAG AAGATGTCGA TATGGAACCG CTACAAGTGG ATGCCTCTCC CATACGCAGC 
GTCCACGGCA ATTTGAATGC CGTGCCGACG GCTACATCCA CGCCCGGACG CAGTCTCTTC 
AGTTTCCGTG GTAGCAACAA GCAAAGCACC GAGGTAGAGC TGGCTGCCCA GACTTCCAGC 
TCGCCGGCAG GAACTCCGGC TCGCACTTTC GCCCAGATCA GCGGCAGGCG TAGCATACGA 
CCCATCGACT CACTGACGCC CTCGAAAATA GGCACCTACA GGTGCGGGAA CAGCGATCTG 
GACACCTCGA ACTGCACGAA CGCAAGCATG AATGCCACAG TCGGATCAGA GATCCCCAAC 
AGCTCGTCAT TTTCATTTTC ATTCTTTGGC CGTGGCCGCA AGCGTGAACG CACTCCCCCA 
CCACTGACGG CCTCTCAGTC GACCAGTGAC CTGGGCCACG ACGTGGATAT GTCGCCGCCA 
AAGCGCGCCC GCTTCGACTT GTTCAGCCTG AATTTGGCTT CTCCCTTTAG CTTGCTGCGC 
TCGAGATTCT CCAAGGCAAC AATCGGTACT CCGACCCGTC TGCGTCTGGA GCCGAACAGT 
TCCGGCGTGG AGGAGCAACA TCTGAACACA TCTACGACTG GCGTACCAGA AGAGGTTGAT 
GTGGAGGAGG AACAACCGGC CGAAGACGTG ACCGTTGGTC AGGAGGCCGG AGCGGAGGAA 
ATCCCCGCCA AGAAGGATGT CGAGGATAAG AACGTCAATG GCCAGGATGG CGACAAGGAG 
TCCAGCGATG GAGAAAACAT TGACCCTGTT GTAGAGGTGG CTGATGTTGC CGCGGTCGCT 
GCCGAGGGCC GATCCATCTG CTCAATTATG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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kuk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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