HisCl1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	HisCl1
Entrez Gene ID	41426
Full Name	Histamine-gated chloride channel subunit 1
Synonyms	CG14723,CT34515,DMHisClalpha2,Dm-HCLB,DmHisCl1,Dmel\CG14723,HA-CI II,HisCl-alpha2,hclB,hisCl1
General protein information	Preferred Names Histamine-gated chloride channel subunit 1 Names CG14723 gene product from transcript CG14723-RA CG14723-PA CG14723-PB CG14723-PC CG14723-PD HisCl1-PA HisCl1-PB HisCl1-PC HisCl1-PD glycine receptor alpha3 subunit-like histamine-gated chloride channel subunit 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 86F7-86F8

mRNA and Protein(s)

mRNA	Protein	Name
NM_141859.4	NP_650116.2	Histamine-gated chloride channel subunit 1, isoform B
NM_206474.3	NP_996196.1	Histamine-gated chloride channel subunit 1, isoform C
NM_169429.4	NP_731632.1	Histamine-gated chloride channel subunit 1, isoform A
NM_001170120.2	NP_001163591.1	Histamine-gated chloride channel subunit 1, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The activity of HisCl1-expressing neurons is acutely and reversibly silenced by exogenous histamine.
Title: Inducible and titratable silencing of Caenorhabditis elegans neurons in vivo with histamine-gated chloride channels.

may play an essential and conserved role in insect neurophysiology
Title: Functional consequences of mutations in the Drosophila histamine receptor HCLB.

These results identify HCLB as an important in vivo target for ivermectin in Drosophila melanogaster, and demonstrate the involvement of this protein in the visual pathway.
Title: Altered ivermectin pharmacology and defective visual system in Drosophila mutants for histamine receptor HCLB.

Histamine-gated chloride channel hclB signaling via the lamina glia plays a previously unrecognized role in shaping the photoreceptor postsynaptic response on large monopolar cells.
Title: Distinct roles for two histamine receptors (hclA and hclB) at the Drosophila photoreceptor synapse.

the genes encoding for proteins involved in histamine signaling [histidine decarboxylase (hdc), histamine-gated chloride channel subunit 1 (hisCl1), ora transientless (ort)] cause abnormal temperature preferences
Title: Histamine and its receptors modulate temperature-preference behaviors in Drosophila.

The following HisCl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HisCl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04579	NM_169429.4 Latest version!	Drosophila melanogaster Histamine-gated chloride channel subunit 1 (HisCl1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa04580	NM_141859.4 Latest version!	Drosophila melanogaster Histamine-gated chloride channel subunit 1 (HisCl1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa04581	NM_206474.3 Latest version!	Drosophila melanogaster Histamine-gated chloride channel subunit 1 (HisCl1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa07779	NM_001170120.2 Latest version!	Drosophila melanogaster Histamine-gated chloride channel subunit 1 (HisCl1), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HisCl1 ( NM_169429.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731632.1 Drosophila melanogaster Histamine-gated chloride channel subunit 1 (HisCl1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa04579
Clone ID Related Accession (Same CDS sequence)	NM_169429.4
Accession Version	NM_169429.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Histamine-gated chloride channel subunit 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_169429.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAAAGCC CAACTAGCAA ATTGGTAGAA TTCAGGTGCC TGATTGCGTT GGCAATATAT 
TTGCACGCGC TGGAGCAATC GATCCAGCAC TGCCATTGTG TTCATGGTTA CAGAAATAAC 
ACGGAGAGCG CCGAGCTGGT CTCCCACTAC GAGTCGAGTC TTTCGCTCCC GGACATTTTG 
CCCATTCCCT CAAAGACGTA CGACAAGAAC CGGGCTCCCA AGCTCCTCGG CCAGCCCACA 
GTAGTCTACT TCCATGTCAC GGTCCTCTCC CTGGACTCCA TTAACGAGGA GTCTATGACC 
TATGTGACGG ACATCTTCCT TGCACAAAGC TGGCGTGATC CTCGCCTGCG GTTGCCTGAG 
AACATGAGTG AGCAGTATCG CATATTGGAT GTCGACTGGT TGCACAGCAT TTGGCGGCCC 
GATTGCTTCT TTAAGAACGC CAAAAAGGTC ACCTTCCATG AGATGAGCAT TCCCAATCAC 
TATCTCTGGC TGTACCACGA CAAAACGCTG CTCTATATGT CCAAACTCAC GTTGGTCCTG 
TCGTGCGCCA TGAAGTTTGA GTCCTATCCG CATGACACGC AAATCTGCTC CATGATGATC 
GAGAGTTTAT CCCATACGGT GGAAGATTTG GTTTTCATTT GGAACATGAC CGACCCACTT 
GTGGTTAACA CGGAGATTGA GTTGCCGCAG CTAGACATAT CAAATAACTA CACAACCGAC 
TGTACTATAG AGTACTCAAC AGGTAACTTC ACCTGCCTGG CCATTGTGTT CAACCTGCGC 
CGACGCCTGG GTTACCATTT GTTCCACACC TACATCCCCT CGGCTCTGAT TGTGGTCATG 
TCTTGGATAT CGTTTTGGAT AAAACCAGAA GCGATACCCG CCCGTGTAAC TCTGGGAGTG 
ACCTCACTGC TAACCCTGGC CACCCAGAAT ACCCAGTCGC AACAATCGCT GCCGCCGGTT 
TCGTATGTCA AGGCTATAGA CGTCTGGATG TCGTCCTGTT CGGTGTTTGT ATTCCTTTCT 
CTGATGGAAT TTGCAGTGGT CAACAATTTT ATGGGACCGG TGGCCACAAA GGCAATGAAG 
GGGTATTCGG ACGAGAACAT CAGTGATCTG GACGACCTAA AGTCTGCACT ACAGCATCAT 
CGGGAATCGA TTATTGAGCC CCAGTACGAC ACTTTCTGCC ATGGCCATGC CACAGCCATT 
TATATAGACA AATTCTCGCG CTTTTTCTTC CCGTTTTCGT TCTTTATACT CAATATTGTC 
TATTGGACAA CGTTCCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731632.1
CDS	317..1597
Translation	MQSPTSKLVEFRCLIALAIYLHALEQSIQHCHCVHGYRNNTESAELVSHYESSLSLPDILPIPSKTYDKNRAPKLLGQPTVVYFHVTVLSLDSINEESMTYVTDIFLAQSWRDPRLRLPENMSEQYRILDVDWLHSIWRPDCFFKNAKKVTFHEMSIPNHYLWLYHDKTLLYMSKLTLVLSCAMKFESYPHDTQICSMMIESLSHTVEDLVFIWNMTDPLVVNTEIELPQLDISNNYTTDCTIEYSTGNFTCLAIVFNLRRRLGYHLFHTYIPSALIVVMSWISFWIKPEAIPARVTLGVTSLLTLATQNTQSQQSLPPVSYVKAIDVWMSSCSVFVFLSLMEFAVVNNFMGPVATKAMKGYSDENISDLDDLKSALQHHRESIIEPQYDTFCHGHATAIYIDKFSRFFFPFSFFILNIVYWTTFL

Target ORF information:

RefSeq Version	NM_169429.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Histamine-gated chloride channel subunit 1 (HisCl1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169429.4

ORF Insert Sequence:

ATGCAAAGCC CAACTAGCAA ATTGGTAGAA TTCAGGTGCC TGATTGCGTT GGCAATATAT 
TTGCACGCGC TGGAGCAATC GATCCAGCAC TGCCATTGTG TTCATGGTTA CAGAAATAAC 
ACGGAGAGCG CCGAGCTGGT CTCCCACTAC GAGTCGAGTC TTTCGCTCCC GGACATTTTG 
CCCATTCCCT CAAAGACGTA CGACAAGAAC CGGGCTCCCA AGCTCCTCGG CCAGCCCACA 
GTAGTCTACT TCCATGTCAC GGTCCTCTCC CTGGACTCCA TTAACGAGGA GTCTATGACC 
TATGTGACGG ACATCTTCCT TGCACAAAGC TGGCGTGATC CTCGCCTGCG GTTGCCTGAG 
AACATGAGTG AGCAGTATCG CATATTGGAT GTCGACTGGT TGCACAGCAT TTGGCGGCCC 
GATTGCTTCT TTAAGAACGC CAAAAAGGTC ACCTTCCATG AGATGAGCAT TCCCAATCAC 
TATCTCTGGC TGTACCACGA CAAAACGCTG CTCTATATGT CCAAACTCAC GTTGGTCCTG 
TCGTGCGCCA TGAAGTTTGA GTCCTATCCG CATGACACGC AAATCTGCTC CATGATGATC 
GAGAGTTTAT CCCATACGGT GGAAGATTTG GTTTTCATTT GGAACATGAC CGACCCACTT 
GTGGTTAACA CGGAGATTGA GTTGCCGCAG CTAGACATAT CAAATAACTA CACAACCGAC 
TGTACTATAG AGTACTCAAC AGGTAACTTC ACCTGCCTGG CCATTGTGTT CAACCTGCGC 
CGACGCCTGG GTTACCATTT GTTCCACACC TACATCCCCT CGGCTCTGAT TGTGGTCATG 
TCTTGGATAT CGTTTTGGAT AAAACCAGAA GCGATACCCG CCCGTGTAAC TCTGGGAGTG 
ACCTCACTGC TAACCCTGGC CACCCAGAAT ACCCAGTCGC AACAATCGCT GCCGCCGGTT 
TCGTATGTCA AGGCTATAGA CGTCTGGATG TCGTCCTGTT CGGTGTTTGT ATTCCTTTCT 
CTGATGGAAT TTGCAGTGGT CAACAATTTT ATGGGACCGG TGGCCACAAA GGCAATGAAG 
GGGTATTCGG ACGAGAACAT CAGTGATCTG GACGACCTAA AGTCTGCACT ACAGCATCAT 
CGGGAATCGA TTATTGAGCC CCAGTACGAC ACTTTCTGCC ATGGCCATGC CACAGCCATT 
TATATAGACA AATTCTCGCG CTTTTTCTTC CCGTTTTCGT TCTTTATACT CAATATTGTC 
TATTGGACAA CGTTCCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HisCl1 ( NM_141859.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650116.2 Drosophila melanogaster Histamine-gated chloride channel subunit 1 (HisCl1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa04580
Clone ID Related Accession (Same CDS sequence)	NM_141859.4
Accession Version	NM_141859.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Histamine-gated chloride channel subunit 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_141859.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAAAGCC CAACTAGCAA ATTGGTAGAA TTCAGGTGCC TGATTGCGTT GGCAATATAT 
TTGCACGCGC TGGAGCAATC GATCCAGCAC TGCCATTGTG TTCATGGTTA CAGAAATAAC 
ACGGAGAGCG CCGAGCTGGT CTCCCACTAC GAGTCGAGTC TTTCGCTCCC GGACATTTTG 
CCCATTCCCT CAAAGACGTA CGACAAGAAC CGGGCTCCCA AGCTCCTCGG CCAGCCCACA 
GTAGTCTACT TCCATGTCAC GGTCCTCTCC CTGGACTCCA TTAACGAGGA GTCTATGACC 
TATGTGACGG ACATCTTCCT TGCACAAAGC TGGCGTGATC CTCGCCTGCG GTTGCCTGAG 
AACATGAGTG AGCAGTATCG CATATTGGAT GTCGACTGGT TGCACAGCAT TTGGCGGCCC 
GATTGCTTCT TTAAGAACGC CAAAAAGGTC ACCTTCCATG AGATGAGCAT TCCCAATCAC 
TATCTCTGGC TGTACCACGA CAAAACGCTG CTCTATATGT CCAAACTCAC GTTGGTCCTG 
TCGTGCGCCA TGAAGTTTGA GTCCTATCCG CATGACACGC AAATCTGCTC CATGATGATC 
GAGAGTTTAT CCCATACGGT GGAAGATTTG GTTTTCATTT GGAACATGAC CGACCCACTT 
GTGGTTAACA CGGAGATTGA GTTGCCGCAG CTAGACATAT CAAATAACTA CACAACCGAC 
TGTACTATAG AGTACTCAAC AGGTAACTTC ACCTGCCTGG CCATTGTGTT CAACCTGCGC 
CGACGCCTGG GTTACCATTT GTTCCACACC TACATCCCCT CGGCTCTGAT TGTGGTCATG 
TCTTGGATAT CGTTTTGGAT AAAACCAGAA GCGATACCCG CCCGTGTAAC TCTGGGAGTG 
ACCTCACTGC TAACCCTGGC CACCCAGAAT ACCCAGTCGC AACAATCGCT GCCGCCGGTT 
TCGTATGTCA AGGCTATAGA CGTCTGGATG TCGTCCTGTT CGGTGTTTGT ATTCCTTTCT 
CTGATGGAAT TTGCAGTGGT CAACAATTTT ATGGGACCGG TGGCCACAAA GGCAATGAAG 
GGGTATTCGG ACGAGAACAT CAGTGATCTG GACGACCTAA AGCATCATCG GGAATCGATT 
ATTGAGCCCC AGTACGACAC TTTCTGCCAT GGCCATGCCA CAGCCATTTA TATAGACAAA 
TTCTCGCGCT TTTTCTTCCC GTTTTCGTTC TTTATACTCA ATATTGTCTA TTGGACAACG 
TTCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650116.2
CDS	317..1585
Translation	MQSPTSKLVEFRCLIALAIYLHALEQSIQHCHCVHGYRNNTESAELVSHYESSLSLPDILPIPSKTYDKNRAPKLLGQPTVVYFHVTVLSLDSINEESMTYVTDIFLAQSWRDPRLRLPENMSEQYRILDVDWLHSIWRPDCFFKNAKKVTFHEMSIPNHYLWLYHDKTLLYMSKLTLVLSCAMKFESYPHDTQICSMMIESLSHTVEDLVFIWNMTDPLVVNTEIELPQLDISNNYTTDCTIEYSTGNFTCLAIVFNLRRRLGYHLFHTYIPSALIVVMSWISFWIKPEAIPARVTLGVTSLLTLATQNTQSQQSLPPVSYVKAIDVWMSSCSVFVFLSLMEFAVVNNFMGPVATKAMKGYSDENISDLDDLKHHRESIIEPQYDTFCHGHATAIYIDKFSRFFFPFSFFILNIVYWTTFL

Target ORF information:

RefSeq Version	NM_141859.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Histamine-gated chloride channel subunit 1 (HisCl1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141859.4

ORF Insert Sequence:

ATGCAAAGCC CAACTAGCAA ATTGGTAGAA TTCAGGTGCC TGATTGCGTT GGCAATATAT 
TTGCACGCGC TGGAGCAATC GATCCAGCAC TGCCATTGTG TTCATGGTTA CAGAAATAAC 
ACGGAGAGCG CCGAGCTGGT CTCCCACTAC GAGTCGAGTC TTTCGCTCCC GGACATTTTG 
CCCATTCCCT CAAAGACGTA CGACAAGAAC CGGGCTCCCA AGCTCCTCGG CCAGCCCACA 
GTAGTCTACT TCCATGTCAC GGTCCTCTCC CTGGACTCCA TTAACGAGGA GTCTATGACC 
TATGTGACGG ACATCTTCCT TGCACAAAGC TGGCGTGATC CTCGCCTGCG GTTGCCTGAG 
AACATGAGTG AGCAGTATCG CATATTGGAT GTCGACTGGT TGCACAGCAT TTGGCGGCCC 
GATTGCTTCT TTAAGAACGC CAAAAAGGTC ACCTTCCATG AGATGAGCAT TCCCAATCAC 
TATCTCTGGC TGTACCACGA CAAAACGCTG CTCTATATGT CCAAACTCAC GTTGGTCCTG 
TCGTGCGCCA TGAAGTTTGA GTCCTATCCG CATGACACGC AAATCTGCTC CATGATGATC 
GAGAGTTTAT CCCATACGGT GGAAGATTTG GTTTTCATTT GGAACATGAC CGACCCACTT 
GTGGTTAACA CGGAGATTGA GTTGCCGCAG CTAGACATAT CAAATAACTA CACAACCGAC 
TGTACTATAG AGTACTCAAC AGGTAACTTC ACCTGCCTGG CCATTGTGTT CAACCTGCGC 
CGACGCCTGG GTTACCATTT GTTCCACACC TACATCCCCT CGGCTCTGAT TGTGGTCATG 
TCTTGGATAT CGTTTTGGAT AAAACCAGAA GCGATACCCG CCCGTGTAAC TCTGGGAGTG 
ACCTCACTGC TAACCCTGGC CACCCAGAAT ACCCAGTCGC AACAATCGCT GCCGCCGGTT 
TCGTATGTCA AGGCTATAGA CGTCTGGATG TCGTCCTGTT CGGTGTTTGT ATTCCTTTCT 
CTGATGGAAT TTGCAGTGGT CAACAATTTT ATGGGACCGG TGGCCACAAA GGCAATGAAG 
GGGTATTCGG ACGAGAACAT CAGTGATCTG GACGACCTAA AGCATCATCG GGAATCGATT 
ATTGAGCCCC AGTACGACAC TTTCTGCCAT GGCCATGCCA CAGCCATTTA TATAGACAAA 
TTCTCGCGCT TTTTCTTCCC GTTTTCGTTC TTTATACTCA ATATTGTCTA TTGGACAACG 
TTCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HisCl1 ( NM_206474.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996196.1 Drosophila melanogaster Histamine-gated chloride channel subunit 1 (HisCl1), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa04581
Clone ID Related Accession (Same CDS sequence)	NM_206474.3
Accession Version	NM_206474.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Histamine-gated chloride channel subunit 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_206474.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAAAGCC CAACTAGCAA ATTGGTAGAA TTCAGGTGCC TGATTGCGTT GGCAATATAT 
TTGCACGCGC TGGAGCAATC GATCCAGCAC TGCCATTGTG TTCATGGTTA CAGAAATAAC 
ACGGAGAGCG CCGAGCTGGT CTCCCACTAC GAGTCGAGTC TTTCGCTCCC GGACATTTTG 
CCCATTCCCT CAAAGACGTA CGACAAGAAC CGGGCTCCCA AGCTCCTCGG CCAGCCCACA 
GTAGTCTACT TCCATGTCAC GGTCCTCTCC CTGGACTCCA TTAACGAGGA GTCTATGACC 
TATGTGACGG ACATCTTCCT TGCACAAAGC TGGCGTGATC CTCGCCTGCG GTTGCCTGAG 
AACATGAGTG AGCAGTATCG CATATTGGAT GTCGACTGGT TGCACAGCAT TTGGCGGCCC 
GATTGCTTCT TTAAGAACGC CAAAAAGGTC ACCTTCCATG AGATGAGCAT TCCCAATCAC 
TATCTCTGGC TGTACCACGA CAAAACGCTG CTCTATATGT CCAAACTCAC GTTGGTCCTG 
TCGTGCGCCA TGAAGTTTGA GTCCTATCCG CATGACACGC AAATCTGCTC CATGATGATC 
GAGAGTTGTA GGTGGAAATG TAATGGTTAT CTACACCCTG CAAATCCCGT ATCGCTTGGA 
ACTTTCCATT CCGCTTCTCG AATTACAAAT AATCTGTGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996196.1
CDS	199..900
Translation	MQSPTSKLVEFRCLIALAIYLHALEQSIQHCHCVHGYRNNTESAELVSHYESSLSLPDILPIPSKTYDKNRAPKLLGQPTVVYFHVTVLSLDSINEESMTYVTDIFLAQSWRDPRLRLPENMSEQYRILDVDWLHSIWRPDCFFKNAKKVTFHEMSIPNHYLWLYHDKTLLYMSKLTLVLSCAMKFESYPHDTQICSMMIESCRWKCNGYLHPANPVSLGTFHSASRITNNLC

Target ORF information:

RefSeq Version	NM_206474.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Histamine-gated chloride channel subunit 1 (HisCl1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206474.3

ORF Insert Sequence:

ATGCAAAGCC CAACTAGCAA ATTGGTAGAA TTCAGGTGCC TGATTGCGTT GGCAATATAT 
TTGCACGCGC TGGAGCAATC GATCCAGCAC TGCCATTGTG TTCATGGTTA CAGAAATAAC 
ACGGAGAGCG CCGAGCTGGT CTCCCACTAC GAGTCGAGTC TTTCGCTCCC GGACATTTTG 
CCCATTCCCT CAAAGACGTA CGACAAGAAC CGGGCTCCCA AGCTCCTCGG CCAGCCCACA 
GTAGTCTACT TCCATGTCAC GGTCCTCTCC CTGGACTCCA TTAACGAGGA GTCTATGACC 
TATGTGACGG ACATCTTCCT TGCACAAAGC TGGCGTGATC CTCGCCTGCG GTTGCCTGAG 
AACATGAGTG AGCAGTATCG CATATTGGAT GTCGACTGGT TGCACAGCAT TTGGCGGCCC 
GATTGCTTCT TTAAGAACGC CAAAAAGGTC ACCTTCCATG AGATGAGCAT TCCCAATCAC 
TATCTCTGGC TGTACCACGA CAAAACGCTG CTCTATATGT CCAAACTCAC GTTGGTCCTG 
TCGTGCGCCA TGAAGTTTGA GTCCTATCCG CATGACACGC AAATCTGCTC CATGATGATC 
GAGAGTTGTA GGTGGAAATG TAATGGTTAT CTACACCCTG CAAATCCCGT ATCGCTTGGA 
ACTTTCCATT CCGCTTCTCG AATTACAAAT AATCTGTGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HisCl1 ( NM_001170120.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163591.1 Drosophila melanogaster Histamine-gated chloride channel subunit 1 (HisCl1), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa07779
Clone ID Related Accession (Same CDS sequence)	NM_001170120.2
Accession Version	NM_001170120.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Histamine-gated chloride channel subunit 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170120.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAAAGCC CAACTAGCAA ATTGGTAGAA TTCAGGTGCC TGATTGCGTT GGCAATATAT 
TTGCACGCGC TGGAGCAATC GATCCAGCAC TGCCATTGTG TTCATGGTTA CAGAAATAAC 
ACGGAGAGCG CCGAGCTGGT CTCCCACTAC GAGTCGAGTC TTTCGCTCCC GGACATTTTG 
CCCATTCCCT CAAAGACGTA CGACAAGAAC CGGGCTCCCA AGCTCCTCGG CCAGCCCACA 
GTAGTCTACT TCCATGTCAC GGTCCTCTCC CTGGACTCCA TTAACGAGGA GTCTATGACC 
TATGTGACGG ACATCTTCCT TGCACAAAGC TGGCGTGATC CTCGCCTGCG GTTGCCTGAG 
AACATGAGTG AGCAGTATCG CATATTGGAT GTCGACTGGT TGCACAGCAT TTGGCGGCCC 
GATTGCTTCT TTAAGAACGC CAAAAAGGTC ACCTTCCATG AGATGAGCAT TCCCAATCAC 
TATCTCTGGC TGTACCACGA CAAAACGCTG CTCTATATGT CCAAACTCAC GTTGGTCCTG 
TCGTGCGCCA TGAAGTTTGA GTCCTATCCG CATGACACGC AAATCTGCTC CATGATGATC 
GAGAGTTATT TGGTTTTCAT TTGGAACATG ACCGACCCAC TTGTGGTTAA CACGGAGATT 
GAGTTGCCGC AGCTAGACAT ATCAAATAAC TACACAACCG ACTGTACTAT AGAGTACTCA 
ACAGGTAACT TCACCTGCCT GGCCATTGTG TTCAACCTGC GCCGACGCCT GGGTTACCAT 
TTGTTCCACA CCTACATCCC CTCGGCTCTG ATTGTGGTCA TGTCTTGGAT ATCGTTTTGG 
ATAAAACCAG AAGCGATACC CGCCCGTGTA ACTCTGGGAG TGACCTCACT GCTAACCCTG 
GCCACCCAGA ATACCCAGTC GCAACAATCG CTGCCGCCGG TTTCGTATGT CAAGGCTATA 
GACGTCTGGA TGTCGTCCTG TTCGGTGTTT GTATTCCTTT CTCTGATGGA ATTTGCAGTG 
GTCAACAATT TTATGGGACC GGTGGCCACA AAGGCAATGA AGGGGTATTC GGACGAGAAC 
ATCAGTGATC TGGACGACCT AAAGCATCAT CGGGAATCGA TTATTGAGCC CCAGTACGAC 
ACTTTCTGCC ATGGCCATGC CACAGCCATT TATATAGACA AATTCTCGCG CTTTTTCTTC 
CCGTTTTCGT TCTTTATACT CAATATTGTC TATTGGACAA CGTTCCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163591.1
CDS	317..1567
Translation	MQSPTSKLVEFRCLIALAIYLHALEQSIQHCHCVHGYRNNTESAELVSHYESSLSLPDILPIPSKTYDKNRAPKLLGQPTVVYFHVTVLSLDSINEESMTYVTDIFLAQSWRDPRLRLPENMSEQYRILDVDWLHSIWRPDCFFKNAKKVTFHEMSIPNHYLWLYHDKTLLYMSKLTLVLSCAMKFESYPHDTQICSMMIESYLVFIWNMTDPLVVNTEIELPQLDISNNYTTDCTIEYSTGNFTCLAIVFNLRRRLGYHLFHTYIPSALIVVMSWISFWIKPEAIPARVTLGVTSLLTLATQNTQSQQSLPPVSYVKAIDVWMSSCSVFVFLSLMEFAVVNNFMGPVATKAMKGYSDENISDLDDLKHHRESIIEPQYDTFCHGHATAIYIDKFSRFFFPFSFFILNIVYWTTFL

Target ORF information:

RefSeq Version	NM_001170120.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Histamine-gated chloride channel subunit 1 (HisCl1), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170120.2

ORF Insert Sequence:

ATGCAAAGCC CAACTAGCAA ATTGGTAGAA TTCAGGTGCC TGATTGCGTT GGCAATATAT 
TTGCACGCGC TGGAGCAATC GATCCAGCAC TGCCATTGTG TTCATGGTTA CAGAAATAAC 
ACGGAGAGCG CCGAGCTGGT CTCCCACTAC GAGTCGAGTC TTTCGCTCCC GGACATTTTG 
CCCATTCCCT CAAAGACGTA CGACAAGAAC CGGGCTCCCA AGCTCCTCGG CCAGCCCACA 
GTAGTCTACT TCCATGTCAC GGTCCTCTCC CTGGACTCCA TTAACGAGGA GTCTATGACC 
TATGTGACGG ACATCTTCCT TGCACAAAGC TGGCGTGATC CTCGCCTGCG GTTGCCTGAG 
AACATGAGTG AGCAGTATCG CATATTGGAT GTCGACTGGT TGCACAGCAT TTGGCGGCCC 
GATTGCTTCT TTAAGAACGC CAAAAAGGTC ACCTTCCATG AGATGAGCAT TCCCAATCAC 
TATCTCTGGC TGTACCACGA CAAAACGCTG CTCTATATGT CCAAACTCAC GTTGGTCCTG 
TCGTGCGCCA TGAAGTTTGA GTCCTATCCG CATGACACGC AAATCTGCTC CATGATGATC 
GAGAGTTATT TGGTTTTCAT TTGGAACATG ACCGACCCAC TTGTGGTTAA CACGGAGATT 
GAGTTGCCGC AGCTAGACAT ATCAAATAAC TACACAACCG ACTGTACTAT AGAGTACTCA 
ACAGGTAACT TCACCTGCCT GGCCATTGTG TTCAACCTGC GCCGACGCCT GGGTTACCAT 
TTGTTCCACA CCTACATCCC CTCGGCTCTG ATTGTGGTCA TGTCTTGGAT ATCGTTTTGG 
ATAAAACCAG AAGCGATACC CGCCCGTGTA ACTCTGGGAG TGACCTCACT GCTAACCCTG 
GCCACCCAGA ATACCCAGTC GCAACAATCG CTGCCGCCGG TTTCGTATGT CAAGGCTATA 
GACGTCTGGA TGTCGTCCTG TTCGGTGTTT GTATTCCTTT CTCTGATGGA ATTTGCAGTG 
GTCAACAATT TTATGGGACC GGTGGCCACA AAGGCAATGA AGGGGTATTC GGACGAGAAC 
ATCAGTGATC TGGACGACCT AAAGCATCAT CGGGAATCGA TTATTGAGCC CCAGTACGAC 
ACTTTCTGCC ATGGCCATGC CACAGCCATT TATATAGACA AATTCTCGCG CTTTTTCTTC 
CCGTTTTCGT TCTTTATACT CAATATTGTC TATTGGACAA CGTTCCTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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HisCl1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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