Leash cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Leash
Entrez Gene ID	41320
Full Name	CG4674 gene product from transcript CG4674-RA
Synonyms	CG32471,CG4674,Dmel\CG4674,leash
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 86C7-86C7

mRNA and Protein(s)

mRNA	Protein	Name
NM_141780.4	NP_650037.2	leash

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Leash gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Leash cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08325	NM_141780.4 Latest version!	Drosophila melanogaster leash (Leash), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Leash ( NM_141780.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650037.2 Drosophila melanogaster leash (Leash), mRNA.

Price & Availability↑

CloneID	OFa08325
Clone ID Related Accession (Same CDS sequence)	NM_141780.4
Accession Version	NM_141780.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	leash
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_141780.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAGCG AGCGGCTGCG AATCACGCTG GACAGCGCGG ATCGGGTTTA CTTTGCGGGC 
GACATAATTC GCGGCGAGAT CTGGATGAAC GTCAACAAGC GCACCAAAAT ACGCGCCATC 
AAGGTGCAGG TCACCGGCAA GGGAAAATGC AAATGGATGG AGATCCTGCG CAAGAACTCG 
AACAACAACG AGTACTCCCG CAGTCTGTTC TACACCAGCA AAGAGGTGTA CGAGCATAGC 
GAGACGCCTC TGCCGGATTT TGAGCCGCGT GGCCTGGAAC TTTCGGCTGG GGAGCACACG 
TTCATCTTCG AGGTGGCGCT GGGTCGCCAG CTACCTTCGA GTTTCAAGGG CAGCTATGGC 
GCCATCAAGT ACAAGATGCG CGTGCTCATC CAGCGACCAT GGACCTTCGA CGAGCGCCAC 
ACGATTCCTT TCACCGTGGT GAAGAACATG CCGCTCCAAC CGATGCAACG CAGCATACCC 
AGTTCGCTGG AGAAGCAGGT GACCAAGACG ATCAGTTTGT TTGGCACTAG ACCGATCACC 
CTGTTGGCCC TTCTGCCCGA AGATTTTGCG GTGCGCGGAG AGCCGCTTAG GATTTGCGCT 
ACTGTGATCA ACAACAGCAC CACCGCTGTG GAGAAGCTGC GCTTTACCGT CCTGCAGTAC 
GTGACCTACT ACAGCCATGT ACCACTGCGG GTGCAGAAGG TGGAGTGCAT AGCGGTGGCC 
ACCAAGGAGA CGGGCTCGGT GCAGAAGAAG ACCGAGCGGA GTTTTGCGCA CGAGCTGCTC 
CTGCCCGATG GAGCCCAGCC GACGGATGAA CAGATGAGCG GCGTCATCAC CATTGTATAC 
GAGCTGCGTG TGGAGGCGGT GCTGCGGGGA TTCTTTAAGA ACTTGATCCT TAATTTGCCC 
TTTAAGGTGT ACTCGCAGGA TCCATCGAAT AGGCAGAGTT CGCTGCGTCC GCCGCCGCCG 
CCACGGCCTA ACGATGGACC ACCTGGTCCG GAATTGGGCT CCGGCAATCT GTTTGAGCCC 
GCCCTGCCGG TCTATCCCTC ACTGGACTCT TCCATTGGCT CGCCCTCGCA CTCATCGCAG 
TTTAGCGAGT GCAGCTCCAT TAACTCGATA ACCTCGGACT CCTCGGCGGC CACTCTGAGT 
CCCGCTGTGG GCGCTGGAAC CGGTGGCATG GATATGTCCT ACACTTCGGG CTCCCAGTCC 
TCGCAGTATG GTAGTCCCTC TGCCAGGACC AGCTTGCGCA GCTCCAATGT GCCCTACATG 
CCCTATTCGT CCAGTCCCCT TATGGTGCAG TCGTATCCGC CACCCCATCT GCCCGCCTAC 
CCGCCGCCGG AGTCGCCGCA ATACTCGCTC CTTGCCCTCA CACCACCGCC GGCCGGAGTG 
GTCGCCGCCC CGCCAACAGG CGGTGCAGCA GCAGGAGGAG CTCCGGCCTA TAGCCAACTG 
CCATCCACCT CGGCCTCAGC ATCGTTCCTG CAGCCTCGCC AGGCGCCTGG AGCCCATGAG 
CTGCCACCTT CATACGAAGA GCTCTTCGGC TTGGCCAACG CACCTCCGGG AACTCCAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650037.2
CDS	434..1996
Translation	MESERLRITLDSADRVYFAGDIIRGEIWMNVNKRTKIRAIKVQVTGKGKCKWMEILRKNSNNNEYSRSLFYTSKEVYEHSETPLPDFEPRGLELSAGEHTFIFEVALGRQLPSSFKGSYGAIKYKMRVLIQRPWTFDERHTIPFTVVKNMPLQPMQRSIPSSLEKQVTKTISLFGTRPITLLALLPEDFAVRGEPLRICATVINNSTTAVEKLRFTVLQYVTYYSHVPLRVQKVECIAVATKETGSVQKKTERSFAHELLLPDGAQPTDEQMSGVITIVYELRVEAVLRGFFKNLILNLPFKVYSQDPSNRQSSLRPPPPPRPNDGPPGPELGSGNLFEPALPVYPSLDSSIGSPSHSSQFSECSSINSITSDSSAATLSPAVGAGTGGMDMSYTSGSQSSQYGSPSARTSLRSSNVPYMPYSSSPLMVQSYPPPHLPAYPPPESPQYSLLALTPPPAGVVAAPPTGGAAAGGAPAYSQLPSTSASASFLQPRQAPGAHELPPSYEELFGLANAPPGTPK

Target ORF information:

RefSeq Version	NM_141780.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster leash (Leash), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141780.4

ORF Insert Sequence:

ATGGAAAGCG AGCGGCTGCG AATCACGCTG GACAGCGCGG ATCGGGTTTA CTTTGCGGGC 
GACATAATTC GCGGCGAGAT CTGGATGAAC GTCAACAAGC GCACCAAAAT ACGCGCCATC 
AAGGTGCAGG TCACCGGCAA GGGAAAATGC AAATGGATGG AGATCCTGCG CAAGAACTCG 
AACAACAACG AGTACTCCCG CAGTCTGTTC TACACCAGCA AAGAGGTGTA CGAGCATAGC 
GAGACGCCTC TGCCGGATTT TGAGCCGCGT GGCCTGGAAC TTTCGGCTGG GGAGCACACG 
TTCATCTTCG AGGTGGCGCT GGGTCGCCAG CTACCTTCGA GTTTCAAGGG CAGCTATGGC 
GCCATCAAGT ACAAGATGCG CGTGCTCATC CAGCGACCAT GGACCTTCGA CGAGCGCCAC 
ACGATTCCTT TCACCGTGGT GAAGAACATG CCGCTCCAAC CGATGCAACG CAGCATACCC 
AGTTCGCTGG AGAAGCAGGT GACCAAGACG ATCAGTTTGT TTGGCACTAG ACCGATCACC 
CTGTTGGCCC TTCTGCCCGA AGATTTTGCG GTGCGCGGAG AGCCGCTTAG GATTTGCGCT 
ACTGTGATCA ACAACAGCAC CACCGCTGTG GAGAAGCTGC GCTTTACCGT CCTGCAGTAC 
GTGACCTACT ACAGCCATGT ACCACTGCGG GTGCAGAAGG TGGAGTGCAT AGCGGTGGCC 
ACCAAGGAGA CGGGCTCGGT GCAGAAGAAG ACCGAGCGGA GTTTTGCGCA CGAGCTGCTC 
CTGCCCGATG GAGCCCAGCC GACGGATGAA CAGATGAGCG GCGTCATCAC CATTGTATAC 
GAGCTGCGTG TGGAGGCGGT GCTGCGGGGA TTCTTTAAGA ACTTGATCCT TAATTTGCCC 
TTTAAGGTGT ACTCGCAGGA TCCATCGAAT AGGCAGAGTT CGCTGCGTCC GCCGCCGCCG 
CCACGGCCTA ACGATGGACC ACCTGGTCCG GAATTGGGCT CCGGCAATCT GTTTGAGCCC 
GCCCTGCCGG TCTATCCCTC ACTGGACTCT TCCATTGGCT CGCCCTCGCA CTCATCGCAG 
TTTAGCGAGT GCAGCTCCAT TAACTCGATA ACCTCGGACT CCTCGGCGGC CACTCTGAGT 
CCCGCTGTGG GCGCTGGAAC CGGTGGCATG GATATGTCCT ACACTTCGGG CTCCCAGTCC 
TCGCAGTATG GTAGTCCCTC TGCCAGGACC AGCTTGCGCA GCTCCAATGT GCCCTACATG 
CCCTATTCGT CCAGTCCCCT TATGGTGCAG TCGTATCCGC CACCCCATCT GCCCGCCTAC 
CCGCCGCCGG AGTCGCCGCA ATACTCGCTC CTTGCCCTCA CACCACCGCC GGCCGGAGTG 
GTCGCCGCCC CGCCAACAGG CGGTGCAGCA GCAGGAGGAG CTCCGGCCTA TAGCCAACTG 
CCATCCACCT CGGCCTCAGC ATCGTTCCTG CAGCCTCGCC AGGCGCCTGG AGCCCATGAG 
CTGCCACCTT CATACGAAGA GCTCTTCGGC TTGGCCAACG CACCTCCGGG AACTCCAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Leash cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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