Hcs cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following Hcs gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hcs cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa07711 NM_001275355.1
Latest version!
Drosophila melanogaster holocarboxylase synthetase, transcript variant B (Hcs), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa22051 NM_001300247.1
Latest version!
Drosophila melanogaster holocarboxylase synthetase, transcript variant D (Hcs), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa03934 NM_141285.2
Latest version!
Drosophila melanogaster holocarboxylase synthetase, transcript variant A (Hcs), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OFa22050 NM_001300246.1
Latest version!
Drosophila melanogaster holocarboxylase synthetase, transcript variant C (Hcs), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa07711
    Clone ID Related Accession (Same CDS sequence) NM_001275355.1
    Accession Version NM_001275355.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2982bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product holocarboxylase synthetase, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTGACCC TGTATTACGT GAGTGCCACC GTCCTGCAGT CCTGGCGCAT TCAAAAGGCG 
    TGCTCCAAGA TCGCCGAGCA CCTCGCGCAG CCCTCGAGCA TCGCCTTCTA TACCCTCCAA
    TCGGGCAGCG ATGACGGATT CGATCCCGCC CTGGCCAGCT CGGAGTTGTG CTGCAATCGG
    AACGCGGCCA AGGTCACGGA CATCCTGTGG CTGCACGCCA ACCAGCGGGG TTGCTGCCTG
    CGTCCGCTGC AAACGCTACA CATCACGCCT TGGATCAGCT TTCCGCCGGC GCCCAGTCTG
    CTTCCCTTCT CCTATGCCGC CGACACGTTA ACGCCGGCGT CCACGCCCAC GGAGGCGGAC
    GTCCCGCGCC AACAACGGGT TTCGCTCTCT GCCCAAGGGG AGGAGCGGAT GCAGCTGCTC
    CTTGAGGCCG ACATAGAGCC ACTACAGCGG CCCAGCTCCG AGGACACATC AGCAGTACGG
    CTGGAAGATT ACGCGGTGCA ACGGGAAGGG GATCCGCAGC CCTTTGAGCT TCTGGCCAAG
    CATCTGAAGC GGCAATCCCG CATAAGCGTC GGTCTGGACA AGGATGGATG GAAGAAGCAC
    ATGGAGGACT TGCGTGCCGT AGGTGTGCTG GCGCACCAGG CCACCGAGTT CGAGTACCAG
    AGGAATCGCT CTGAGGGAAG AACGAAATCG GATCCCACCA CACATGATCA ACGCCCGACT
    TCGGAGCTTT TGGCCAAGGC TGTGGCCGTG GTGGAGCCCA CCAACAAGGC ATATCTGTCT
    CCGGCAAGGA CGACAGAAGC CTCGCTCAAA GCCGAGGCCA AGGGAACGTC AACAAAACCT
    TTTCCGACTA AAAGCGATGC CAAACCCGCC ACTCTTGCCA AGAGCGAGGG CACGCCAGCC
    ACAAGCAAAG AGGACTCCAA GTTAACGCCA ACTAAGGGTG CATTAAAGAC AAGCGAACTA
    GAGAAACTAG CGGCTGTCCA GGCAGCGCAG CAGCAGAAGA AAGAGGCAGT CCAGGTCTCT
    CCCGTCAAGG CTGCTTTCTT AGCTAAACCA CCTATGCTAA GTAAACATGA TGAGCCTGAT
    AAGGCATCAG ATCAGCCAAC GAAGCCCAGG AAATCCTTTG AGGCGGTAAA GCCGTTAAAT
    TCTCCTCCGT CATCCAGAGC TGCTCCCTCG CGTCCTGTGC TGCAACGCAA CAAGGATAGC
    CTGCAGGACG CAAAACCATT GAATGTTTTG GTCTACTCAG ACAGCGCCAG TGCGCGGGAA
    TCCACATTGG CCACTCTGCA GCAGCTGCTG GAGCGCAATG TGTACACGAT CTATCCTCTG
    ATGCCTCAGC AAGCGGCTCA AAAATACTGG ACGGAACAGA CTGCGTTGCT GGTCGTCTGC
    GGATCGGTGG CACACGGAAT TGGGCAGATA TTGGTGGACT ACTTTCTGCA GGGCGGCAAG
    GTGTTAAGCC TGTGCTCGGA TATACTGCAC TTTGTACTCC CCAACTATCG CACAGCGGAG
    GTTCGAGAGC ACGAATTGGT TCAGTTCTCC TATGACAAGT GGCAGCGGGT CAAGATGATG
    CACCACATCT TTTGCTACCA ACCGTCGCCG GTGAAGAAGC ACTTCTCCAC TGACAGCGAG
    GAGTCCACCA AGTCGCACTC TCGCAAACCG TCCATGGAAC TGAAGGATCT GGCCGGTCAC
    AGCCACAATT TGGATGTCCA TGTGCTGGGA ACCGAGGAAA CCTGGAATAC GCCCAGCTTG
    ATGTTGGCCA AAAGCCTGCA GAGCGGCGGA AAGGCCGTCT TCTCCCAAGT GCACTTGGAA
    ATGAATCCCA GCGAATTTGA ATCCGATGAG ACTAAGTACT CCATTCTAAA GCAAAACGAG
    CGAACTCGCC TCGAGATATT TGCGGATCTC CTGGGAAAGT ACTTGGATGT GCAGGTCCGC
    GGTGGAGACG GTGTCGACCA GCCGCAGCCT GGAGTTGTCT ACAAGCACGC ATATTTCCTA
    GGTCGGCACG AGGCCAAGTT TGAGCTTCTG GAGAAGCTAC GTCTGCGCTG CAGCGGATCC
    GACAACGTGA TAGCAACGCC GAACCTGACG ATGAAGTTCT GTGGCAAGGA CGATAAGCCT
    CCAGTGGCCA ACAACAATGT CCTGCCCATA CTCATACATT CCTGTCCGGA TGACTTCTCC
    ACCGTGGATT ACTTCGATAA TCTTAAGACG GAACACATCG GACGACTGGT CATCTATGCA
    CCGGTGGTCA GCAGCTCCAT GCATCTCATA AACAACCTGG AGCTCATCCA CGGCCTGGCT
    GTGCTCCCCG TGCAGCAGAC GTCCGGAGTG GGTCGCAGAA ATAATCAATG GCTTAGTCCG
    CCGGGCTGTG CCATGTTCTC GCTGCAGCTG CACCTGACCA TGGACAGCGC TTTGAGCTCT
    CGGCTGCCTC TGTTGCAGCA CCTTGTTGGA ACGGCGATTG TTAATTCGCT CCGCAGTCAT
    GAAGAGTACG GGGTGCTGGA TATTTCCATT AAGTGGCCGA ATGACATTTA TGCAAATGGA
    AATCAGAAAA TTGGGGGCCT CGTCATTAAT ACCACACTTC AGGGATCCCA AGCCATCGTC
    AATATCGGAA GTGGAATTAA TTTGAACAAT TCAAGACCAA CAGTTTGCAT CAATGACTTG
    ATAAGAGAAT ATAATACCCG TGTCCCGAAC AACAAGTTAC CTATACTTAA GTACGAGCTA
    CTTATTGCCA TGATATTCAA TGAAATCGAA AGACTTTTGG GAGAAGTACA AAACGGAGAC
    TTTGATAGTT TTTATGCATT ATACTATTCG CTATGGTTAC ACAGCGGGCA ATCTGTTAAG
    ATTTGCCTAC AAAAAGATCA GGAAAAAGAG GCCGAAATTG TGGGAATCGA TGACTTTGGA
    TTTTTGGAGG TGAAATTACC AACTGGTACT ATAGAAATTG TGCAGCCCGA TGGAAACAGC
    TTTGATATGT TGAAGGGATT GATTATACCC AAGTATCAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001262284.1
    CDS362..3343
    Translation

    Target ORF information:

    RefSeq Version NM_001275355.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster holocarboxylase synthetase, transcript variant B (Hcs), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001275355.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTGACCC TGTATTACGT GAGTGCCACC GTCCTGCAGT CCTGGCGCAT TCAAAAGGCG 
    TGCTCCAAGA TCGCCGAGCA CCTCGCGCAG CCCTCGAGCA TCGCCTTCTA TACCCTCCAA
    TCGGGCAGCG ATGACGGATT CGATCCCGCC CTGGCCAGCT CGGAGTTGTG CTGCAATCGG
    AACGCGGCCA AGGTCACGGA CATCCTGTGG CTGCACGCCA ACCAGCGGGG TTGCTGCCTG
    CGTCCGCTGC AAACGCTACA CATCACGCCT TGGATCAGCT TTCCGCCGGC GCCCAGTCTG
    CTTCCCTTCT CCTATGCCGC CGACACGTTA ACGCCGGCGT CCACGCCCAC GGAGGCGGAC
    GTCCCGCGCC AACAACGGGT TTCGCTCTCT GCCCAAGGGG AGGAGCGGAT GCAGCTGCTC
    CTTGAGGCCG ACATAGAGCC ACTACAGCGG CCCAGCTCCG AGGACACATC AGCAGTACGG
    CTGGAAGATT ACGCGGTGCA ACGGGAAGGG GATCCGCAGC CCTTTGAGCT TCTGGCCAAG
    CATCTGAAGC GGCAATCCCG CATAAGCGTC GGTCTGGACA AGGATGGATG GAAGAAGCAC
    ATGGAGGACT TGCGTGCCGT AGGTGTGCTG GCGCACCAGG CCACCGAGTT CGAGTACCAG
    AGGAATCGCT CTGAGGGAAG AACGAAATCG GATCCCACCA CACATGATCA ACGCCCGACT
    TCGGAGCTTT TGGCCAAGGC TGTGGCCGTG GTGGAGCCCA CCAACAAGGC ATATCTGTCT
    CCGGCAAGGA CGACAGAAGC CTCGCTCAAA GCCGAGGCCA AGGGAACGTC AACAAAACCT
    TTTCCGACTA AAAGCGATGC CAAACCCGCC ACTCTTGCCA AGAGCGAGGG CACGCCAGCC
    ACAAGCAAAG AGGACTCCAA GTTAACGCCA ACTAAGGGTG CATTAAAGAC AAGCGAACTA
    GAGAAACTAG CGGCTGTCCA GGCAGCGCAG CAGCAGAAGA AAGAGGCAGT CCAGGTCTCT
    CCCGTCAAGG CTGCTTTCTT AGCTAAACCA CCTATGCTAA GTAAACATGA TGAGCCTGAT
    AAGGCATCAG ATCAGCCAAC GAAGCCCAGG AAATCCTTTG AGGCGGTAAA GCCGTTAAAT
    TCTCCTCCGT CATCCAGAGC TGCTCCCTCG CGTCCTGTGC TGCAACGCAA CAAGGATAGC
    CTGCAGGACG CAAAACCATT GAATGTTTTG GTCTACTCAG ACAGCGCCAG TGCGCGGGAA
    TCCACATTGG CCACTCTGCA GCAGCTGCTG GAGCGCAATG TGTACACGAT CTATCCTCTG
    ATGCCTCAGC AAGCGGCTCA AAAATACTGG ACGGAACAGA CTGCGTTGCT GGTCGTCTGC
    GGATCGGTGG CACACGGAAT TGGGCAGATA TTGGTGGACT ACTTTCTGCA GGGCGGCAAG
    GTGTTAAGCC TGTGCTCGGA TATACTGCAC TTTGTACTCC CCAACTATCG CACAGCGGAG
    GTTCGAGAGC ACGAATTGGT TCAGTTCTCC TATGACAAGT GGCAGCGGGT CAAGATGATG
    CACCACATCT TTTGCTACCA ACCGTCGCCG GTGAAGAAGC ACTTCTCCAC TGACAGCGAG
    GAGTCCACCA AGTCGCACTC TCGCAAACCG TCCATGGAAC TGAAGGATCT GGCCGGTCAC
    AGCCACAATT TGGATGTCCA TGTGCTGGGA ACCGAGGAAA CCTGGAATAC GCCCAGCTTG
    ATGTTGGCCA AAAGCCTGCA GAGCGGCGGA AAGGCCGTCT TCTCCCAAGT GCACTTGGAA
    ATGAATCCCA GCGAATTTGA ATCCGATGAG ACTAAGTACT CCATTCTAAA GCAAAACGAG
    CGAACTCGCC TCGAGATATT TGCGGATCTC CTGGGAAAGT ACTTGGATGT GCAGGTCCGC
    GGTGGAGACG GTGTCGACCA GCCGCAGCCT GGAGTTGTCT ACAAGCACGC ATATTTCCTA
    GGTCGGCACG AGGCCAAGTT TGAGCTTCTG GAGAAGCTAC GTCTGCGCTG CAGCGGATCC
    GACAACGTGA TAGCAACGCC GAACCTGACG ATGAAGTTCT GTGGCAAGGA CGATAAGCCT
    CCAGTGGCCA ACAACAATGT CCTGCCCATA CTCATACATT CCTGTCCGGA TGACTTCTCC
    ACCGTGGATT ACTTCGATAA TCTTAAGACG GAACACATCG GACGACTGGT CATCTATGCA
    CCGGTGGTCA GCAGCTCCAT GCATCTCATA AACAACCTGG AGCTCATCCA CGGCCTGGCT
    GTGCTCCCCG TGCAGCAGAC GTCCGGAGTG GGTCGCAGAA ATAATCAATG GCTTAGTCCG
    CCGGGCTGTG CCATGTTCTC GCTGCAGCTG CACCTGACCA TGGACAGCGC TTTGAGCTCT
    CGGCTGCCTC TGTTGCAGCA CCTTGTTGGA ACGGCGATTG TTAATTCGCT CCGCAGTCAT
    GAAGAGTACG GGGTGCTGGA TATTTCCATT AAGTGGCCGA ATGACATTTA TGCAAATGGA
    AATCAGAAAA TTGGGGGCCT CGTCATTAAT ACCACACTTC AGGGATCCCA AGCCATCGTC
    AATATCGGAA GTGGAATTAA TTTGAACAAT TCAAGACCAA CAGTTTGCAT CAATGACTTG
    ATAAGAGAAT ATAATACCCG TGTCCCGAAC AACAAGTTAC CTATACTTAA GTACGAGCTA
    CTTATTGCCA TGATATTCAA TGAAATCGAA AGACTTTTGG GAGAAGTACA AAACGGAGAC
    TTTGATAGTT TTTATGCATT ATACTATTCG CTATGGTTAC ACAGCGGGCA ATCTGTTAAG
    ATTTGCCTAC AAAAAGATCA GGAAAAAGAG GCCGAAATTG TGGGAATCGA TGACTTTGGA
    TTTTTGGAGG TGAAATTACC AACTGGTACT ATAGAAATTG TGCAGCCCGA TGGAAACAGC
    TTTGATATGT TGAAGGGATT GATTATACCC AAGTATCAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa22051
    Clone ID Related Accession (Same CDS sequence) NM_001300247.1
    Accession Version NM_001300247.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3000bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product holocarboxylase synthetase, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTGACCC TGTATTACGT GAGTGCCACC GTCCTGCAGT CCTGGCGCAT TCAAAAGGCG 
    TGCTCCAAGA TCGCCGAGCA CCTCGCGCAG CCCTCGAGCA TCGCCTTCTA TACCCTCCAA
    TCGGGCAGCG ATGACGGATT CGATCCCGCC CTGGCCAGCT CGGAGTTGTG CTGCAATCGG
    AACGCGGCCA AGGTCACGGA CATCCTGTGG CTGCACGCCA ACCAGCGGGG TTGCTGCCTG
    CGTCCGCTGC AAACGCTACA CATCACGCCT TGGATCAGCT TTCCGCCGGC GCCCAGTCTG
    CTTCCCTTCT CCTATGCCGC CGACACGTTA ACGCCGGCGT CCACGCCCAC GGAGGCGGAC
    GTCCCGCGCC AACAACGGGT TTCGCTCTCT GCCCAAGGGG AGGAGCGGAT GCAGCTGCTC
    CTTGAGGCCG ACATAGAGCC ACTACAGCGG CCCAGCTCCG AGGACACATC AGCAGTACGG
    CTGGAAGATT ACGCGGTGCA ACGGGAAGGG GATCCGCAGC CCTTTGAGCT TCTGGCCAAG
    CATCTGAAGC GGCAATCCCG CATAAGCGTC GGTCTGGACA AGGATGGATG GAAGAAGCAC
    ATGGAGGACT TGCGTGCCGT AGGTGTGCTG GCGCACCAGG CCACCGAGTT CGAGTACCAG
    AGGAATCGCT CTGAGGGAAG AACGAAATCG GATCCCACCA CACATGATCA ACGCCCGACT
    TCGGAGCTTT TGGCCAAGGC TGTGGCCGTG GTGGAGCCCA CCAACAAGGC ATATCTGTCT
    CCGGCAAGGA CGACAGAAGC CTCGCTCAAA GCCGAGGCCA AGGGAACGTC AACAAAACCT
    TTTCCGACTA AAAGCGATGC CAAACCCGCC ACTCTTGCCA AGAGCGAGGG CACGCCAGCC
    ACAAGCAAAG AGGACTCCAA GTTAACGCCA ACTAAGGGTG CATTAAAGAC AAGCGAACTA
    GAGAAACTAG CGGCTGTCCA GGCAGCGCAG CAGCAGAAGA AAGAGGCAGT CCAGGTCTCT
    CCCGTCAAGG CTGCTTTCTT AGCTAAACCA CCTATGCTAA GTAAACATGA TGAGCCTGAT
    AAGGCATCAG ATCAGCCAAC GAAGCCCAGG AAATCCTTTG AGGCGGTAAA GCCGTTAAAT
    TCTCCTCCGT CATCCAGAGC TGCTCCCTCG CGTCCTGTGC TGCAACGCAA CAAGGATAGC
    CTGCAGGACG CAAAACCATT GAATGTTTTG GTCTACTCAG ACAGCGCCAG TGCGCGGGAA
    TCCACATTGG CCACTCTGCA GCAGCTGCTG GAGCGCAATG TGTACACGAT CTATCCTCTG
    ATGCCTCAGC AAGCGGCTCA AAAATACTGG ACGGAACAGA CTGCGTTGCT GGTCGTCTGC
    GGATCGGTGG CACACGGAAT TGGGCAGATA TTGGTGGACT ACTTTCTGCA GGGCGGCAAG
    GTGTTAAGCC TGTGCTCGGA TATACTGCAC TTTGTACTCC CCAACTATCG CACAGCGGAG
    GTTCGAGAGC ACGAATTGGT TCAGTTCTCC TATGACAAGT GGCAGCGGGT CAAGATGATG
    CACCACATCT TTTGCTACCA ACCGTCGCCG GTGAAGAAGC ACTTCTCCAC TGACAGCGAG
    GAGTCCACCA AGTCGCACTC TCGCAAACCA GCTCTAGTAT GTCCAAGGTC CATGGAACTG
    AAGGATCTGG CCGGTCACAG CCACAATTTG GATGTCCATG TGCTGGGAAC CGAGGAAACC
    TGGAATACGC CCAGCTTGAT GTTGGCCAAA AGCCTGCAGA GCGGCGGAAA GGCCGTCTTC
    TCCCAAGTGC ACTTGGAAAT GAATCCCAGC GAATTTGAAT CCGATGAGAC TAAGTACTCC
    ATTCTAAAGC AAAACGAGCG AACTCGCCTC GAGATATTTG CGGATCTCCT GGGAAAGTAC
    TTGGATGTGC AGGTCCGCGG TGGAGACGGT GTCGACCAGC CGCAGCCTGG AGTTGTCTAC
    AAGCACGCAT ATTTCCTAGG TCGGCACGAG GCCAAGTTTG AGCTTCTGGA GAAGCTACGT
    CTGCGCTGCA GCGGATCCGA CAACGTGATA GCAACGCCGA ACCTGACGAT GAAGTTCTGT
    GGCAAGGACG ATAAGCCTCC AGTGGCCAAC AACAATGTCC TGCCCATACT CATACATTCC
    TGTCCGGATG ACTTCTCCAC CGTGGATTAC TTCGATAATC TTAAGACGGA ACACATCGGA
    CGACTGGTCA TCTATGCACC GGTGGTCAGC AGCTCCATGC ATCTCATAAA CAACCTGGAG
    CTCATCCACG GCCTGGCTGT GCTCCCCGTG CAGCAGACGT CCGGAGTGGG TCGCAGAAAT
    AATCAATGGC TTAGTCCGCC GGGCTGTGCC ATGTTCTCGC TGCAGCTGCA CCTGACCATG
    GACAGCGCTT TGAGCTCTCG GCTGCCTCTG TTGCAGCACC TTGTTGGAAC GGCGATTGTT
    AATTCGCTCC GCAGTCATGA AGAGTACGGG GTGCTGGATA TTTCCATTAA GTGGCCGAAT
    GACATTTATG CAAATGGAAA TCAGAAAATT GGGGGCCTCG TCATTAATAC CACACTTCAG
    GGATCCCAAG CCATCGTCAA TATCGGAAGT GGAATTAATT TGAACAATTC AAGACCAACA
    GTTTGCATCA ATGACTTGAT AAGAGAATAT AATACCCGTG TCCCGAACAA CAAGTTACCT
    ATACTTAAGT ACGAGCTACT TATTGCCATG ATATTCAATG AAATCGAAAG ACTTTTGGGA
    GAAGTACAAA ACGGAGACTT TGATAGTTTT TATGCATTAT ACTATTCGCT ATGGTTACAC
    AGCGGGCAAT CTGTTAAGAT TTGCCTACAA AAAGATCAGG AAAAAGAGGC CGAAATTGTG
    GGAATCGATG ACTTTGGATT TTTGGAGGTG AAATTACCAA CTGGTACTAT AGAAATTGTG
    CAGCCCGATG GAAACAGCTT TGATATGTTG AAGGGATTGA TTATACCCAA GTATCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001287176.1
    CDS362..3361
    Translation

    Target ORF information:

    RefSeq Version NM_001300247.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster holocarboxylase synthetase, transcript variant D (Hcs), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001300247.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTGACCC TGTATTACGT GAGTGCCACC GTCCTGCAGT CCTGGCGCAT TCAAAAGGCG 
    TGCTCCAAGA TCGCCGAGCA CCTCGCGCAG CCCTCGAGCA TCGCCTTCTA TACCCTCCAA
    TCGGGCAGCG ATGACGGATT CGATCCCGCC CTGGCCAGCT CGGAGTTGTG CTGCAATCGG
    AACGCGGCCA AGGTCACGGA CATCCTGTGG CTGCACGCCA ACCAGCGGGG TTGCTGCCTG
    CGTCCGCTGC AAACGCTACA CATCACGCCT TGGATCAGCT TTCCGCCGGC GCCCAGTCTG
    CTTCCCTTCT CCTATGCCGC CGACACGTTA ACGCCGGCGT CCACGCCCAC GGAGGCGGAC
    GTCCCGCGCC AACAACGGGT TTCGCTCTCT GCCCAAGGGG AGGAGCGGAT GCAGCTGCTC
    CTTGAGGCCG ACATAGAGCC ACTACAGCGG CCCAGCTCCG AGGACACATC AGCAGTACGG
    CTGGAAGATT ACGCGGTGCA ACGGGAAGGG GATCCGCAGC CCTTTGAGCT TCTGGCCAAG
    CATCTGAAGC GGCAATCCCG CATAAGCGTC GGTCTGGACA AGGATGGATG GAAGAAGCAC
    ATGGAGGACT TGCGTGCCGT AGGTGTGCTG GCGCACCAGG CCACCGAGTT CGAGTACCAG
    AGGAATCGCT CTGAGGGAAG AACGAAATCG GATCCCACCA CACATGATCA ACGCCCGACT
    TCGGAGCTTT TGGCCAAGGC TGTGGCCGTG GTGGAGCCCA CCAACAAGGC ATATCTGTCT
    CCGGCAAGGA CGACAGAAGC CTCGCTCAAA GCCGAGGCCA AGGGAACGTC AACAAAACCT
    TTTCCGACTA AAAGCGATGC CAAACCCGCC ACTCTTGCCA AGAGCGAGGG CACGCCAGCC
    ACAAGCAAAG AGGACTCCAA GTTAACGCCA ACTAAGGGTG CATTAAAGAC AAGCGAACTA
    GAGAAACTAG CGGCTGTCCA GGCAGCGCAG CAGCAGAAGA AAGAGGCAGT CCAGGTCTCT
    CCCGTCAAGG CTGCTTTCTT AGCTAAACCA CCTATGCTAA GTAAACATGA TGAGCCTGAT
    AAGGCATCAG ATCAGCCAAC GAAGCCCAGG AAATCCTTTG AGGCGGTAAA GCCGTTAAAT
    TCTCCTCCGT CATCCAGAGC TGCTCCCTCG CGTCCTGTGC TGCAACGCAA CAAGGATAGC
    CTGCAGGACG CAAAACCATT GAATGTTTTG GTCTACTCAG ACAGCGCCAG TGCGCGGGAA
    TCCACATTGG CCACTCTGCA GCAGCTGCTG GAGCGCAATG TGTACACGAT CTATCCTCTG
    ATGCCTCAGC AAGCGGCTCA AAAATACTGG ACGGAACAGA CTGCGTTGCT GGTCGTCTGC
    GGATCGGTGG CACACGGAAT TGGGCAGATA TTGGTGGACT ACTTTCTGCA GGGCGGCAAG
    GTGTTAAGCC TGTGCTCGGA TATACTGCAC TTTGTACTCC CCAACTATCG CACAGCGGAG
    GTTCGAGAGC ACGAATTGGT TCAGTTCTCC TATGACAAGT GGCAGCGGGT CAAGATGATG
    CACCACATCT TTTGCTACCA ACCGTCGCCG GTGAAGAAGC ACTTCTCCAC TGACAGCGAG
    GAGTCCACCA AGTCGCACTC TCGCAAACCA GCTCTAGTAT GTCCAAGGTC CATGGAACTG
    AAGGATCTGG CCGGTCACAG CCACAATTTG GATGTCCATG TGCTGGGAAC CGAGGAAACC
    TGGAATACGC CCAGCTTGAT GTTGGCCAAA AGCCTGCAGA GCGGCGGAAA GGCCGTCTTC
    TCCCAAGTGC ACTTGGAAAT GAATCCCAGC GAATTTGAAT CCGATGAGAC TAAGTACTCC
    ATTCTAAAGC AAAACGAGCG AACTCGCCTC GAGATATTTG CGGATCTCCT GGGAAAGTAC
    TTGGATGTGC AGGTCCGCGG TGGAGACGGT GTCGACCAGC CGCAGCCTGG AGTTGTCTAC
    AAGCACGCAT ATTTCCTAGG TCGGCACGAG GCCAAGTTTG AGCTTCTGGA GAAGCTACGT
    CTGCGCTGCA GCGGATCCGA CAACGTGATA GCAACGCCGA ACCTGACGAT GAAGTTCTGT
    GGCAAGGACG ATAAGCCTCC AGTGGCCAAC AACAATGTCC TGCCCATACT CATACATTCC
    TGTCCGGATG ACTTCTCCAC CGTGGATTAC TTCGATAATC TTAAGACGGA ACACATCGGA
    CGACTGGTCA TCTATGCACC GGTGGTCAGC AGCTCCATGC ATCTCATAAA CAACCTGGAG
    CTCATCCACG GCCTGGCTGT GCTCCCCGTG CAGCAGACGT CCGGAGTGGG TCGCAGAAAT
    AATCAATGGC TTAGTCCGCC GGGCTGTGCC ATGTTCTCGC TGCAGCTGCA CCTGACCATG
    GACAGCGCTT TGAGCTCTCG GCTGCCTCTG TTGCAGCACC TTGTTGGAAC GGCGATTGTT
    AATTCGCTCC GCAGTCATGA AGAGTACGGG GTGCTGGATA TTTCCATTAA GTGGCCGAAT
    GACATTTATG CAAATGGAAA TCAGAAAATT GGGGGCCTCG TCATTAATAC CACACTTCAG
    GGATCCCAAG CCATCGTCAA TATCGGAAGT GGAATTAATT TGAACAATTC AAGACCAACA
    GTTTGCATCA ATGACTTGAT AAGAGAATAT AATACCCGTG TCCCGAACAA CAAGTTACCT
    ATACTTAAGT ACGAGCTACT TATTGCCATG ATATTCAATG AAATCGAAAG ACTTTTGGGA
    GAAGTACAAA ACGGAGACTT TGATAGTTTT TATGCATTAT ACTATTCGCT ATGGTTACAC
    AGCGGGCAAT CTGTTAAGAT TTGCCTACAA AAAGATCAGG AAAAAGAGGC CGAAATTGTG
    GGAATCGATG ACTTTGGATT TTTGGAGGTG AAATTACCAA CTGGTACTAT AGAAATTGTG
    CAGCCCGATG GAAACAGCTT TGATATGTTG AAGGGATTGA TTATACCCAA GTATCAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa03934
    Clone ID Related Accession (Same CDS sequence) NM_141285.2
    Accession Version NM_141285.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3126bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product holocarboxylase synthetase, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141285.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTGACCC TGTATTACGT GAGTGCCACC GTCCTGCAGT CCTGGCGCAT TCAAAAGGCG 
    TGCTCCAAGA TCGCCGAGCA CCTCGCGCAG CCCTCGAGCA TCGCCTTCTA TACCCTCCAA
    TCGGGCAGCG ATGACGGATT CGATCCCGCC CTGGCCAGCT CGGAGTTGTG CTGCAATCGG
    AACGCGGCCA AGGTCACGGA CATCCTGTGG CTGCACGCCA ACCAGCGGGG TTGCTGCCTG
    CGTCCGCTGC AAACGCTACA CATCACGCCT TGGATCAGCT TTCCGCCGGC GCCCAGTCTG
    CTTCCCTTCT CCTATGCCGC CGACACGTTA ACGCCGGCGT CCACGCCCAC GGAGGCGGAC
    GTCCCGCGCC AACAACGGGT TTCGCTCTCT GCCCAAGGGG AGGAGCGGAT GCAGCTGCTC
    CTTGAGGCCG ACATAGAGCC ACTACAGCGG CCCAGCTCCG AGGACACATC AGCAGTACGG
    CTGGAAGATT ACGGTAAGCT AATTGCTTGG AAAATAGATT CCCACCTGGC GGTGCTCATC
    GAAACAGACG TCGAGCACTT CACGAAACTT TTGATAACGA CTTTCTTGAG AAATAATTTA
    TGCATTAACG ATCAGCTGCC ACTTTTGCGG ATTGAGTCGG TGCAACGGGA AGGGGATCCG
    CAGCCCTTTG AGCTTCTGGC CAAGCATCTG AAGCGGCAAT CCCGCATAAG CGTCGGTCTG
    GACAAGGATG GATGGAAGAA GCACATGGAG GACTTGCGTG CCGTAGGTGT GCTGGCGCAC
    CAGGCCACCG AGTTCGAGTA CCAGAGGAAT CGCTCTGAGG GAAGAACGAA ATCGGATCCC
    ACCACACATG ATCAACGCCC GACTTCGGAG CTTTTGGCCA AGGCTGTGGC CGTGGTGGAG
    CCCACCAACA AGGCATATCT GTCTCCGGCA AGGACGACAG AAGCCTCGCT CAAAGCCGAG
    GCCAAGGGAA CGTCAACAAA ACCTTTTCCG ACTAAAAGCG ATGCCAAACC CGCCACTCTT
    GCCAAGAGCG AGGGCACGCC AGCCACAAGC AAAGAGGACT CCAAGTTAAC GCCAACTAAG
    GGTGCATTAA AGACAAGCGA ACTAGAGAAA CTAGCGGCTG TCCAGGCAGC GCAGCAGCAG
    AAGAAAGAGG CAGTCCAGGT CTCTCCCGTC AAGGCTGCTT TCTTAGCTAA ACCACCTATG
    CTAAGTAAAC ATGATGAGCC TGATAAGGCA TCAGATCAGC CAACGAAGCC CAGGAAATCC
    TTTGAGGCGG TAAAGCCGTT AAATTCTCCT CCGTCATCCA GAGCTGCTCC CTCGCGTCCT
    GTGCTGCAAC GCAACAAGGA TAGCCTGCAG GACGCAAAAC CATTGAATGT TTTGGTCTAC
    TCAGACAGCG CCAGTGCGCG GGAATCCACA TTGGCCACTC TGCAGCAGCT GCTGGAGCGC
    AATGTGTACA CGATCTATCC TCTGATGCCT CAGCAAGCGG CTCAAAAATA CTGGACGGAA
    CAGACTGCGT TGCTGGTCGT CTGCGGATCG GTGGCACACG GAATTGGGCA GATATTGGTG
    GACTACTTTC TGCAGGGCGG CAAGGTGTTA AGCCTGTGCT CGGATATACT GCACTTTGTA
    CTCCCCAACT ATCGCACAGC GGAGGTTCGA GAGCACGAAT TGGTTCAGTT CTCCTATGAC
    AAGTGGCAGC GGGTCAAGAT GATGCACCAC ATCTTTTGCT ACCAACCGTC GCCGGTGAAG
    AAGCACTTCT CCACTGACAG CGAGGAGTCC ACCAAGTCGC ACTCTCGCAA ACCGTCCATG
    GAACTGAAGG ATCTGGCCGG TCACAGCCAC AATTTGGATG TCCATGTGCT GGGAACCGAG
    GAAACCTGGA ATACGCCCAG CTTGATGTTG GCCAAAAGCC TGCAGAGCGG CGGAAAGGCC
    GTCTTCTCCC AAGTGCACTT GGAAATGAAT CCCAGCGAAT TTGAATCCGA TGAGACTAAG
    TACTCCATTC TAAAGCAAAA CGAGCGAACT CGCCTCGAGA TATTTGCGGA TCTCCTGGGA
    AAGTACTTGG ATGTGCAGGT CCGCGGTGGA GACGGTGTCG ACCAGCCGCA GCCTGGAGTT
    GTCTACAAGC ACGCATATTT CCTAGGTCGG CACGAGGCCA AGTTTGAGCT TCTGGAGAAG
    CTACGTCTGC GCTGCAGCGG ATCCGACAAC GTGATAGCAA CGCCGAACCT GACGATGAAG
    TTCTGTGGCA AGGACGATAA GCCTCCAGTG GCCAACAACA ATGTCCTGCC CATACTCATA
    CATTCCTGTC CGGATGACTT CTCCACCGTG GATTACTTCG ATAATCTTAA GACGGAACAC
    ATCGGACGAC TGGTCATCTA TGCACCGGTG GTCAGCAGCT CCATGCATCT CATAAACAAC
    CTGGAGCTCA TCCACGGCCT GGCTGTGCTC CCCGTGCAGC AGACGTCCGG AGTGGGTCGC
    AGAAATAATC AATGGCTTAG TCCGCCGGGC TGTGCCATGT TCTCGCTGCA GCTGCACCTG
    ACCATGGACA GCGCTTTGAG CTCTCGGCTG CCTCTGTTGC AGCACCTTGT TGGAACGGCG
    ATTGTTAATT CGCTCCGCAG TCATGAAGAG TACGGGGTGC TGGATATTTC CATTAAGTGG
    CCGAATGACA TTTATGCAAA TGGAAATCAG AAAATTGGGG GCCTCGTCAT TAATACCACA
    CTTCAGGGAT CCCAAGCCAT CGTCAATATC GGAAGTGGAA TTAATTTGAA CAATTCAAGA
    CCAACAGTTT GCATCAATGA CTTGATAAGA GAATATAATA CCCGTGTCCC GAACAACAAG
    TTACCTATAC TTAAGTACGA GCTACTTATT GCCATGATAT TCAATGAAAT CGAAAGACTT
    TTGGGAGAAG TACAAAACGG AGACTTTGAT AGTTTTTATG CATTATACTA TTCGCTATGG
    TTACACAGCG GGCAATCTGT TAAGATTTGC CTACAAAAAG ATCAGGAAAA AGAGGCCGAA
    ATTGTGGGAA TCGATGACTT TGGATTTTTG GAGGTGAAAT TACCAACTGG TACTATAGAA
    ATTGTGCAGC CCGATGGAAA CAGCTTTGAT ATGTTGAAGG GATTGATTAT ACCCAAGTAT
    CAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_649542.1
    CDS362..3487
    Translation

    Target ORF information:

    RefSeq Version NM_141285.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster holocarboxylase synthetase, transcript variant A (Hcs), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_141285.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTGACCC TGTATTACGT GAGTGCCACC GTCCTGCAGT CCTGGCGCAT TCAAAAGGCG 
    TGCTCCAAGA TCGCCGAGCA CCTCGCGCAG CCCTCGAGCA TCGCCTTCTA TACCCTCCAA
    TCGGGCAGCG ATGACGGATT CGATCCCGCC CTGGCCAGCT CGGAGTTGTG CTGCAATCGG
    AACGCGGCCA AGGTCACGGA CATCCTGTGG CTGCACGCCA ACCAGCGGGG TTGCTGCCTG
    CGTCCGCTGC AAACGCTACA CATCACGCCT TGGATCAGCT TTCCGCCGGC GCCCAGTCTG
    CTTCCCTTCT CCTATGCCGC CGACACGTTA ACGCCGGCGT CCACGCCCAC GGAGGCGGAC
    GTCCCGCGCC AACAACGGGT TTCGCTCTCT GCCCAAGGGG AGGAGCGGAT GCAGCTGCTC
    CTTGAGGCCG ACATAGAGCC ACTACAGCGG CCCAGCTCCG AGGACACATC AGCAGTACGG
    CTGGAAGATT ACGGTAAGCT AATTGCTTGG AAAATAGATT CCCACCTGGC GGTGCTCATC
    GAAACAGACG TCGAGCACTT CACGAAACTT TTGATAACGA CTTTCTTGAG AAATAATTTA
    TGCATTAACG ATCAGCTGCC ACTTTTGCGG ATTGAGTCGG TGCAACGGGA AGGGGATCCG
    CAGCCCTTTG AGCTTCTGGC CAAGCATCTG AAGCGGCAAT CCCGCATAAG CGTCGGTCTG
    GACAAGGATG GATGGAAGAA GCACATGGAG GACTTGCGTG CCGTAGGTGT GCTGGCGCAC
    CAGGCCACCG AGTTCGAGTA CCAGAGGAAT CGCTCTGAGG GAAGAACGAA ATCGGATCCC
    ACCACACATG ATCAACGCCC GACTTCGGAG CTTTTGGCCA AGGCTGTGGC CGTGGTGGAG
    CCCACCAACA AGGCATATCT GTCTCCGGCA AGGACGACAG AAGCCTCGCT CAAAGCCGAG
    GCCAAGGGAA CGTCAACAAA ACCTTTTCCG ACTAAAAGCG ATGCCAAACC CGCCACTCTT
    GCCAAGAGCG AGGGCACGCC AGCCACAAGC AAAGAGGACT CCAAGTTAAC GCCAACTAAG
    GGTGCATTAA AGACAAGCGA ACTAGAGAAA CTAGCGGCTG TCCAGGCAGC GCAGCAGCAG
    AAGAAAGAGG CAGTCCAGGT CTCTCCCGTC AAGGCTGCTT TCTTAGCTAA ACCACCTATG
    CTAAGTAAAC ATGATGAGCC TGATAAGGCA TCAGATCAGC CAACGAAGCC CAGGAAATCC
    TTTGAGGCGG TAAAGCCGTT AAATTCTCCT CCGTCATCCA GAGCTGCTCC CTCGCGTCCT
    GTGCTGCAAC GCAACAAGGA TAGCCTGCAG GACGCAAAAC CATTGAATGT TTTGGTCTAC
    TCAGACAGCG CCAGTGCGCG GGAATCCACA TTGGCCACTC TGCAGCAGCT GCTGGAGCGC
    AATGTGTACA CGATCTATCC TCTGATGCCT CAGCAAGCGG CTCAAAAATA CTGGACGGAA
    CAGACTGCGT TGCTGGTCGT CTGCGGATCG GTGGCACACG GAATTGGGCA GATATTGGTG
    GACTACTTTC TGCAGGGCGG CAAGGTGTTA AGCCTGTGCT CGGATATACT GCACTTTGTA
    CTCCCCAACT ATCGCACAGC GGAGGTTCGA GAGCACGAAT TGGTTCAGTT CTCCTATGAC
    AAGTGGCAGC GGGTCAAGAT GATGCACCAC ATCTTTTGCT ACCAACCGTC GCCGGTGAAG
    AAGCACTTCT CCACTGACAG CGAGGAGTCC ACCAAGTCGC ACTCTCGCAA ACCGTCCATG
    GAACTGAAGG ATCTGGCCGG TCACAGCCAC AATTTGGATG TCCATGTGCT GGGAACCGAG
    GAAACCTGGA ATACGCCCAG CTTGATGTTG GCCAAAAGCC TGCAGAGCGG CGGAAAGGCC
    GTCTTCTCCC AAGTGCACTT GGAAATGAAT CCCAGCGAAT TTGAATCCGA TGAGACTAAG
    TACTCCATTC TAAAGCAAAA CGAGCGAACT CGCCTCGAGA TATTTGCGGA TCTCCTGGGA
    AAGTACTTGG ATGTGCAGGT CCGCGGTGGA GACGGTGTCG ACCAGCCGCA GCCTGGAGTT
    GTCTACAAGC ACGCATATTT CCTAGGTCGG CACGAGGCCA AGTTTGAGCT TCTGGAGAAG
    CTACGTCTGC GCTGCAGCGG ATCCGACAAC GTGATAGCAA CGCCGAACCT GACGATGAAG
    TTCTGTGGCA AGGACGATAA GCCTCCAGTG GCCAACAACA ATGTCCTGCC CATACTCATA
    CATTCCTGTC CGGATGACTT CTCCACCGTG GATTACTTCG ATAATCTTAA GACGGAACAC
    ATCGGACGAC TGGTCATCTA TGCACCGGTG GTCAGCAGCT CCATGCATCT CATAAACAAC
    CTGGAGCTCA TCCACGGCCT GGCTGTGCTC CCCGTGCAGC AGACGTCCGG AGTGGGTCGC
    AGAAATAATC AATGGCTTAG TCCGCCGGGC TGTGCCATGT TCTCGCTGCA GCTGCACCTG
    ACCATGGACA GCGCTTTGAG CTCTCGGCTG CCTCTGTTGC AGCACCTTGT TGGAACGGCG
    ATTGTTAATT CGCTCCGCAG TCATGAAGAG TACGGGGTGC TGGATATTTC CATTAAGTGG
    CCGAATGACA TTTATGCAAA TGGAAATCAG AAAATTGGGG GCCTCGTCAT TAATACCACA
    CTTCAGGGAT CCCAAGCCAT CGTCAATATC GGAAGTGGAA TTAATTTGAA CAATTCAAGA
    CCAACAGTTT GCATCAATGA CTTGATAAGA GAATATAATA CCCGTGTCCC GAACAACAAG
    TTACCTATAC TTAAGTACGA GCTACTTATT GCCATGATAT TCAATGAAAT CGAAAGACTT
    TTGGGAGAAG TACAAAACGG AGACTTTGAT AGTTTTTATG CATTATACTA TTCGCTATGG
    TTACACAGCG GGCAATCTGT TAAGATTTGC CTACAAAAAG ATCAGGAAAA AGAGGCCGAA
    ATTGTGGGAA TCGATGACTT TGGATTTTTG GAGGTGAAAT TACCAACTGG TACTATAGAA
    ATTGTGCAGC CCGATGGAAA CAGCTTTGAT ATGTTGAAGG GATTGATTAT ACCCAAGTAT
    CAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa22050
    Clone ID Related Accession (Same CDS sequence) NM_001300246.1
    Accession Version NM_001300246.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3144bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product holocarboxylase synthetase, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTGACCC TGTATTACGT GAGTGCCACC GTCCTGCAGT CCTGGCGCAT TCAAAAGGCG 
    TGCTCCAAGA TCGCCGAGCA CCTCGCGCAG CCCTCGAGCA TCGCCTTCTA TACCCTCCAA
    TCGGGCAGCG ATGACGGATT CGATCCCGCC CTGGCCAGCT CGGAGTTGTG CTGCAATCGG
    AACGCGGCCA AGGTCACGGA CATCCTGTGG CTGCACGCCA ACCAGCGGGG TTGCTGCCTG
    CGTCCGCTGC AAACGCTACA CATCACGCCT TGGATCAGCT TTCCGCCGGC GCCCAGTCTG
    CTTCCCTTCT CCTATGCCGC CGACACGTTA ACGCCGGCGT CCACGCCCAC GGAGGCGGAC
    GTCCCGCGCC AACAACGGGT TTCGCTCTCT GCCCAAGGGG AGGAGCGGAT GCAGCTGCTC
    CTTGAGGCCG ACATAGAGCC ACTACAGCGG CCCAGCTCCG AGGACACATC AGCAGTACGG
    CTGGAAGATT ACGGTAAGCT AATTGCTTGG AAAATAGATT CCCACCTGGC GGTGCTCATC
    GAAACAGACG TCGAGCACTT CACGAAACTT TTGATAACGA CTTTCTTGAG AAATAATTTA
    TGCATTAACG ATCAGCTGCC ACTTTTGCGG ATTGAGTCGG TGCAACGGGA AGGGGATCCG
    CAGCCCTTTG AGCTTCTGGC CAAGCATCTG AAGCGGCAAT CCCGCATAAG CGTCGGTCTG
    GACAAGGATG GATGGAAGAA GCACATGGAG GACTTGCGTG CCGTAGGTGT GCTGGCGCAC
    CAGGCCACCG AGTTCGAGTA CCAGAGGAAT CGCTCTGAGG GAAGAACGAA ATCGGATCCC
    ACCACACATG ATCAACGCCC GACTTCGGAG CTTTTGGCCA AGGCTGTGGC CGTGGTGGAG
    CCCACCAACA AGGCATATCT GTCTCCGGCA AGGACGACAG AAGCCTCGCT CAAAGCCGAG
    GCCAAGGGAA CGTCAACAAA ACCTTTTCCG ACTAAAAGCG ATGCCAAACC CGCCACTCTT
    GCCAAGAGCG AGGGCACGCC AGCCACAAGC AAAGAGGACT CCAAGTTAAC GCCAACTAAG
    GGTGCATTAA AGACAAGCGA ACTAGAGAAA CTAGCGGCTG TCCAGGCAGC GCAGCAGCAG
    AAGAAAGAGG CAGTCCAGGT CTCTCCCGTC AAGGCTGCTT TCTTAGCTAA ACCACCTATG
    CTAAGTAAAC ATGATGAGCC TGATAAGGCA TCAGATCAGC CAACGAAGCC CAGGAAATCC
    TTTGAGGCGG TAAAGCCGTT AAATTCTCCT CCGTCATCCA GAGCTGCTCC CTCGCGTCCT
    GTGCTGCAAC GCAACAAGGA TAGCCTGCAG GACGCAAAAC CATTGAATGT TTTGGTCTAC
    TCAGACAGCG CCAGTGCGCG GGAATCCACA TTGGCCACTC TGCAGCAGCT GCTGGAGCGC
    AATGTGTACA CGATCTATCC TCTGATGCCT CAGCAAGCGG CTCAAAAATA CTGGACGGAA
    CAGACTGCGT TGCTGGTCGT CTGCGGATCG GTGGCACACG GAATTGGGCA GATATTGGTG
    GACTACTTTC TGCAGGGCGG CAAGGTGTTA AGCCTGTGCT CGGATATACT GCACTTTGTA
    CTCCCCAACT ATCGCACAGC GGAGGTTCGA GAGCACGAAT TGGTTCAGTT CTCCTATGAC
    AAGTGGCAGC GGGTCAAGAT GATGCACCAC ATCTTTTGCT ACCAACCGTC GCCGGTGAAG
    AAGCACTTCT CCACTGACAG CGAGGAGTCC ACCAAGTCGC ACTCTCGCAA ACCAGCTCTA
    GTATGTCCAA GGTCCATGGA ACTGAAGGAT CTGGCCGGTC ACAGCCACAA TTTGGATGTC
    CATGTGCTGG GAACCGAGGA AACCTGGAAT ACGCCCAGCT TGATGTTGGC CAAAAGCCTG
    CAGAGCGGCG GAAAGGCCGT CTTCTCCCAA GTGCACTTGG AAATGAATCC CAGCGAATTT
    GAATCCGATG AGACTAAGTA CTCCATTCTA AAGCAAAACG AGCGAACTCG CCTCGAGATA
    TTTGCGGATC TCCTGGGAAA GTACTTGGAT GTGCAGGTCC GCGGTGGAGA CGGTGTCGAC
    CAGCCGCAGC CTGGAGTTGT CTACAAGCAC GCATATTTCC TAGGTCGGCA CGAGGCCAAG
    TTTGAGCTTC TGGAGAAGCT ACGTCTGCGC TGCAGCGGAT CCGACAACGT GATAGCAACG
    CCGAACCTGA CGATGAAGTT CTGTGGCAAG GACGATAAGC CTCCAGTGGC CAACAACAAT
    GTCCTGCCCA TACTCATACA TTCCTGTCCG GATGACTTCT CCACCGTGGA TTACTTCGAT
    AATCTTAAGA CGGAACACAT CGGACGACTG GTCATCTATG CACCGGTGGT CAGCAGCTCC
    ATGCATCTCA TAAACAACCT GGAGCTCATC CACGGCCTGG CTGTGCTCCC CGTGCAGCAG
    ACGTCCGGAG TGGGTCGCAG AAATAATCAA TGGCTTAGTC CGCCGGGCTG TGCCATGTTC
    TCGCTGCAGC TGCACCTGAC CATGGACAGC GCTTTGAGCT CTCGGCTGCC TCTGTTGCAG
    CACCTTGTTG GAACGGCGAT TGTTAATTCG CTCCGCAGTC ATGAAGAGTA CGGGGTGCTG
    GATATTTCCA TTAAGTGGCC GAATGACATT TATGCAAATG GAAATCAGAA AATTGGGGGC
    CTCGTCATTA ATACCACACT TCAGGGATCC CAAGCCATCG TCAATATCGG AAGTGGAATT
    AATTTGAACA ATTCAAGACC AACAGTTTGC ATCAATGACT TGATAAGAGA ATATAATACC
    CGTGTCCCGA ACAACAAGTT ACCTATACTT AAGTACGAGC TACTTATTGC CATGATATTC
    AATGAAATCG AAAGACTTTT GGGAGAAGTA CAAAACGGAG ACTTTGATAG TTTTTATGCA
    TTATACTATT CGCTATGGTT ACACAGCGGG CAATCTGTTA AGATTTGCCT ACAAAAAGAT
    CAGGAAAAAG AGGCCGAAAT TGTGGGAATC GATGACTTTG GATTTTTGGA GGTGAAATTA
    CCAACTGGTA CTATAGAAAT TGTGCAGCCC GATGGAAACA GCTTTGATAT GTTGAAGGGA
    TTGATTATAC CCAAGTATCA ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001287175.1
    CDS362..3505
    Translation

    Target ORF information:

    RefSeq Version NM_001300246.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster holocarboxylase synthetase, transcript variant C (Hcs), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001300246.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTGACCC TGTATTACGT GAGTGCCACC GTCCTGCAGT CCTGGCGCAT TCAAAAGGCG 
    TGCTCCAAGA TCGCCGAGCA CCTCGCGCAG CCCTCGAGCA TCGCCTTCTA TACCCTCCAA
    TCGGGCAGCG ATGACGGATT CGATCCCGCC CTGGCCAGCT CGGAGTTGTG CTGCAATCGG
    AACGCGGCCA AGGTCACGGA CATCCTGTGG CTGCACGCCA ACCAGCGGGG TTGCTGCCTG
    CGTCCGCTGC AAACGCTACA CATCACGCCT TGGATCAGCT TTCCGCCGGC GCCCAGTCTG
    CTTCCCTTCT CCTATGCCGC CGACACGTTA ACGCCGGCGT CCACGCCCAC GGAGGCGGAC
    GTCCCGCGCC AACAACGGGT TTCGCTCTCT GCCCAAGGGG AGGAGCGGAT GCAGCTGCTC
    CTTGAGGCCG ACATAGAGCC ACTACAGCGG CCCAGCTCCG AGGACACATC AGCAGTACGG
    CTGGAAGATT ACGGTAAGCT AATTGCTTGG AAAATAGATT CCCACCTGGC GGTGCTCATC
    GAAACAGACG TCGAGCACTT CACGAAACTT TTGATAACGA CTTTCTTGAG AAATAATTTA
    TGCATTAACG ATCAGCTGCC ACTTTTGCGG ATTGAGTCGG TGCAACGGGA AGGGGATCCG
    CAGCCCTTTG AGCTTCTGGC CAAGCATCTG AAGCGGCAAT CCCGCATAAG CGTCGGTCTG
    GACAAGGATG GATGGAAGAA GCACATGGAG GACTTGCGTG CCGTAGGTGT GCTGGCGCAC
    CAGGCCACCG AGTTCGAGTA CCAGAGGAAT CGCTCTGAGG GAAGAACGAA ATCGGATCCC
    ACCACACATG ATCAACGCCC GACTTCGGAG CTTTTGGCCA AGGCTGTGGC CGTGGTGGAG
    CCCACCAACA AGGCATATCT GTCTCCGGCA AGGACGACAG AAGCCTCGCT CAAAGCCGAG
    GCCAAGGGAA CGTCAACAAA ACCTTTTCCG ACTAAAAGCG ATGCCAAACC CGCCACTCTT
    GCCAAGAGCG AGGGCACGCC AGCCACAAGC AAAGAGGACT CCAAGTTAAC GCCAACTAAG
    GGTGCATTAA AGACAAGCGA ACTAGAGAAA CTAGCGGCTG TCCAGGCAGC GCAGCAGCAG
    AAGAAAGAGG CAGTCCAGGT CTCTCCCGTC AAGGCTGCTT TCTTAGCTAA ACCACCTATG
    CTAAGTAAAC ATGATGAGCC TGATAAGGCA TCAGATCAGC CAACGAAGCC CAGGAAATCC
    TTTGAGGCGG TAAAGCCGTT AAATTCTCCT CCGTCATCCA GAGCTGCTCC CTCGCGTCCT
    GTGCTGCAAC GCAACAAGGA TAGCCTGCAG GACGCAAAAC CATTGAATGT TTTGGTCTAC
    TCAGACAGCG CCAGTGCGCG GGAATCCACA TTGGCCACTC TGCAGCAGCT GCTGGAGCGC
    AATGTGTACA CGATCTATCC TCTGATGCCT CAGCAAGCGG CTCAAAAATA CTGGACGGAA
    CAGACTGCGT TGCTGGTCGT CTGCGGATCG GTGGCACACG GAATTGGGCA GATATTGGTG
    GACTACTTTC TGCAGGGCGG CAAGGTGTTA AGCCTGTGCT CGGATATACT GCACTTTGTA
    CTCCCCAACT ATCGCACAGC GGAGGTTCGA GAGCACGAAT TGGTTCAGTT CTCCTATGAC
    AAGTGGCAGC GGGTCAAGAT GATGCACCAC ATCTTTTGCT ACCAACCGTC GCCGGTGAAG
    AAGCACTTCT CCACTGACAG CGAGGAGTCC ACCAAGTCGC ACTCTCGCAA ACCAGCTCTA
    GTATGTCCAA GGTCCATGGA ACTGAAGGAT CTGGCCGGTC ACAGCCACAA TTTGGATGTC
    CATGTGCTGG GAACCGAGGA AACCTGGAAT ACGCCCAGCT TGATGTTGGC CAAAAGCCTG
    CAGAGCGGCG GAAAGGCCGT CTTCTCCCAA GTGCACTTGG AAATGAATCC CAGCGAATTT
    GAATCCGATG AGACTAAGTA CTCCATTCTA AAGCAAAACG AGCGAACTCG CCTCGAGATA
    TTTGCGGATC TCCTGGGAAA GTACTTGGAT GTGCAGGTCC GCGGTGGAGA CGGTGTCGAC
    CAGCCGCAGC CTGGAGTTGT CTACAAGCAC GCATATTTCC TAGGTCGGCA CGAGGCCAAG
    TTTGAGCTTC TGGAGAAGCT ACGTCTGCGC TGCAGCGGAT CCGACAACGT GATAGCAACG
    CCGAACCTGA CGATGAAGTT CTGTGGCAAG GACGATAAGC CTCCAGTGGC CAACAACAAT
    GTCCTGCCCA TACTCATACA TTCCTGTCCG GATGACTTCT CCACCGTGGA TTACTTCGAT
    AATCTTAAGA CGGAACACAT CGGACGACTG GTCATCTATG CACCGGTGGT CAGCAGCTCC
    ATGCATCTCA TAAACAACCT GGAGCTCATC CACGGCCTGG CTGTGCTCCC CGTGCAGCAG
    ACGTCCGGAG TGGGTCGCAG AAATAATCAA TGGCTTAGTC CGCCGGGCTG TGCCATGTTC
    TCGCTGCAGC TGCACCTGAC CATGGACAGC GCTTTGAGCT CTCGGCTGCC TCTGTTGCAG
    CACCTTGTTG GAACGGCGAT TGTTAATTCG CTCCGCAGTC ATGAAGAGTA CGGGGTGCTG
    GATATTTCCA TTAAGTGGCC GAATGACATT TATGCAAATG GAAATCAGAA AATTGGGGGC
    CTCGTCATTA ATACCACACT TCAGGGATCC CAAGCCATCG TCAATATCGG AAGTGGAATT
    AATTTGAACA ATTCAAGACC AACAGTTTGC ATCAATGACT TGATAAGAGA ATATAATACC
    CGTGTCCCGA ACAACAAGTT ACCTATACTT AAGTACGAGC TACTTATTGC CATGATATTC
    AATGAAATCG AAAGACTTTT GGGAGAAGTA CAAAACGGAG ACTTTGATAG TTTTTATGCA
    TTATACTATT CGCTATGGTT ACACAGCGGG CAATCTGTTA AGATTTGCCT ACAAAAAGAT
    CAGGAAAAAG AGGCCGAAAT TGTGGGAATC GATGACTTTG GATTTTTGGA GGTGAAATTA
    CCAACTGGTA CTATAGAAAT TGTGCAGCCC GATGGAAACA GCTTTGATAT GTTGAAGGGA
    TTGATTATAC CCAAGTATCA ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.