Hcs cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hcs
Entrez Gene ID	40659
Full Name	Holocarboxylase synthetase
Synonyms	CG14670,Dmel\CG14670,HCS,HSC,NEST:bs27h05
General protein information	Preferred Names Holocarboxylase synthetase Names CG14670 gene product from transcript CG14670-RC CG14670-PA CG14670-PB CG14670-PC CG14670-PD Hcs-PA Hcs-PB Hcs-PC Hcs-PD holocarboxylase synthetase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 83A5-83A5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001300247.1	NP_001287176.1	holocarboxylase synthetase, isoform D
NM_141285.2	NP_649542.1	holocarboxylase synthetase, isoform A
NM_001275355.1	NP_001262284.1	holocarboxylase synthetase, isoform B
NM_001300246.1	NP_001287175.1	holocarboxylase synthetase, isoform C

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Hcs	NP_649542.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001481	XP_321640.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HCS may have a role in the repression of gene expression through a mechanism involving its trafficking to the nucleus and interaction with heterochromatic sites coincident with H3K9met3.
Title: Trafficking and chromatin dynamics of holocarboxylase synthetase during development of Drosophila melanogaster.

Gene expression patterns and phenotypes in holocarboxylase synthetase-deficient flies in previous studies are caused by decreased biotinylation of histones, not 3-methylcrotonyl-CoA carboxylase.
Title: Susceptibility to heat stress and aberrant gene expression patterns in holocarboxylase synthetase-deficient Drosophila melanogaster are caused by decreased biotinylation of histones, not of carboxylases.

The following Hcs gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hcs cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa07711	NM_001275355.1 Latest version!	Drosophila melanogaster holocarboxylase synthetase, transcript variant B (Hcs), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa22051	NM_001300247.1 Latest version!	Drosophila melanogaster holocarboxylase synthetase, transcript variant D (Hcs), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa03934	NM_141285.2 Latest version!	Drosophila melanogaster holocarboxylase synthetase, transcript variant A (Hcs), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa22050	NM_001300246.1 Latest version!	Drosophila melanogaster holocarboxylase synthetase, transcript variant C (Hcs), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hcs ( NM_001275355.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262284.1 Drosophila melanogaster holocarboxylase synthetase, transcript variant B (Hcs), mRNA.

Price & Availability↑

CloneID	OFa07711
Clone ID Related Accession (Same CDS sequence)	NM_001275355.1
Accession Version	NM_001275355.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	holocarboxylase synthetase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGACCC TGTATTACGT GAGTGCCACC GTCCTGCAGT CCTGGCGCAT TCAAAAGGCG 
TGCTCCAAGA TCGCCGAGCA CCTCGCGCAG CCCTCGAGCA TCGCCTTCTA TACCCTCCAA 
TCGGGCAGCG ATGACGGATT CGATCCCGCC CTGGCCAGCT CGGAGTTGTG CTGCAATCGG 
AACGCGGCCA AGGTCACGGA CATCCTGTGG CTGCACGCCA ACCAGCGGGG TTGCTGCCTG 
CGTCCGCTGC AAACGCTACA CATCACGCCT TGGATCAGCT TTCCGCCGGC GCCCAGTCTG 
CTTCCCTTCT CCTATGCCGC CGACACGTTA ACGCCGGCGT CCACGCCCAC GGAGGCGGAC 
GTCCCGCGCC AACAACGGGT TTCGCTCTCT GCCCAAGGGG AGGAGCGGAT GCAGCTGCTC 
CTTGAGGCCG ACATAGAGCC ACTACAGCGG CCCAGCTCCG AGGACACATC AGCAGTACGG 
CTGGAAGATT ACGCGGTGCA ACGGGAAGGG GATCCGCAGC CCTTTGAGCT TCTGGCCAAG 
CATCTGAAGC GGCAATCCCG CATAAGCGTC GGTCTGGACA AGGATGGATG GAAGAAGCAC 
ATGGAGGACT TGCGTGCCGT AGGTGTGCTG GCGCACCAGG CCACCGAGTT CGAGTACCAG 
AGGAATCGCT CTGAGGGAAG AACGAAATCG GATCCCACCA CACATGATCA ACGCCCGACT 
TCGGAGCTTT TGGCCAAGGC TGTGGCCGTG GTGGAGCCCA CCAACAAGGC ATATCTGTCT 
CCGGCAAGGA CGACAGAAGC CTCGCTCAAA GCCGAGGCCA AGGGAACGTC AACAAAACCT 
TTTCCGACTA AAAGCGATGC CAAACCCGCC ACTCTTGCCA AGAGCGAGGG CACGCCAGCC 
ACAAGCAAAG AGGACTCCAA GTTAACGCCA ACTAAGGGTG CATTAAAGAC AAGCGAACTA 
GAGAAACTAG CGGCTGTCCA GGCAGCGCAG CAGCAGAAGA AAGAGGCAGT CCAGGTCTCT 
CCCGTCAAGG CTGCTTTCTT AGCTAAACCA CCTATGCTAA GTAAACATGA TGAGCCTGAT 
AAGGCATCAG ATCAGCCAAC GAAGCCCAGG AAATCCTTTG AGGCGGTAAA GCCGTTAAAT 
TCTCCTCCGT CATCCAGAGC TGCTCCCTCG CGTCCTGTGC TGCAACGCAA CAAGGATAGC 
CTGCAGGACG CAAAACCATT GAATGTTTTG GTCTACTCAG ACAGCGCCAG TGCGCGGGAA 
TCCACATTGG CCACTCTGCA GCAGCTGCTG GAGCGCAATG TGTACACGAT CTATCCTCTG 
ATGCCTCAGC AAGCGGCTCA AAAATACTGG ACGGAACAGA CTGCGTTGCT GGTCGTCTGC 
GGATCGGTGG CACACGGAAT TGGGCAGATA TTGGTGGACT ACTTTCTGCA GGGCGGCAAG 
GTGTTAAGCC TGTGCTCGGA TATACTGCAC TTTGTACTCC CCAACTATCG CACAGCGGAG 
GTTCGAGAGC ACGAATTGGT TCAGTTCTCC TATGACAAGT GGCAGCGGGT CAAGATGATG 
CACCACATCT TTTGCTACCA ACCGTCGCCG GTGAAGAAGC ACTTCTCCAC TGACAGCGAG 
GAGTCCACCA AGTCGCACTC TCGCAAACCG TCCATGGAAC TGAAGGATCT GGCCGGTCAC 
AGCCACAATT TGGATGTCCA TGTGCTGGGA ACCGAGGAAA CCTGGAATAC GCCCAGCTTG 
ATGTTGGCCA AAAGCCTGCA GAGCGGCGGA AAGGCCGTCT TCTCCCAAGT GCACTTGGAA 
ATGAATCCCA GCGAATTTGA ATCCGATGAG ACTAAGTACT CCATTCTAAA GCAAAACGAG 
CGAACTCGCC TCGAGATATT TGCGGATCTC CTGGGAAAGT ACTTGGATGT GCAGGTCCGC 
GGTGGAGACG GTGTCGACCA GCCGCAGCCT GGAGTTGTCT ACAAGCACGC ATATTTCCTA 
GGTCGGCACG AGGCCAAGTT TGAGCTTCTG GAGAAGCTAC GTCTGCGCTG CAGCGGATCC 
GACAACGTGA TAGCAACGCC GAACCTGACG ATGAAGTTCT GTGGCAAGGA CGATAAGCCT 
CCAGTGGCCA ACAACAATGT CCTGCCCATA CTCATACATT CCTGTCCGGA TGACTTCTCC 
ACCGTGGATT ACTTCGATAA TCTTAAGACG GAACACATCG GACGACTGGT CATCTATGCA 
CCGGTGGTCA GCAGCTCCAT GCATCTCATA AACAACCTGG AGCTCATCCA CGGCCTGGCT 
GTGCTCCCCG TGCAGCAGAC GTCCGGAGTG GGTCGCAGAA ATAATCAATG GCTTAGTCCG 
CCGGGCTGTG CCATGTTCTC GCTGCAGCTG CACCTGACCA TGGACAGCGC TTTGAGCTCT 
CGGCTGCCTC TGTTGCAGCA CCTTGTTGGA ACGGCGATTG TTAATTCGCT CCGCAGTCAT 
GAAGAGTACG GGGTGCTGGA TATTTCCATT AAGTGGCCGA ATGACATTTA TGCAAATGGA 
AATCAGAAAA TTGGGGGCCT CGTCATTAAT ACCACACTTC AGGGATCCCA AGCCATCGTC 
AATATCGGAA GTGGAATTAA TTTGAACAAT TCAAGACCAA CAGTTTGCAT CAATGACTTG 
ATAAGAGAAT ATAATACCCG TGTCCCGAAC AACAAGTTAC CTATACTTAA GTACGAGCTA 
CTTATTGCCA TGATATTCAA TGAAATCGAA AGACTTTTGG GAGAAGTACA AAACGGAGAC 
TTTGATAGTT TTTATGCATT ATACTATTCG CTATGGTTAC ACAGCGGGCA ATCTGTTAAG 
ATTTGCCTAC AAAAAGATCA GGAAAAAGAG GCCGAAATTG TGGGAATCGA TGACTTTGGA 
TTTTTGGAGG TGAAATTACC AACTGGTACT ATAGAAATTG TGCAGCCCGA TGGAAACAGC 
TTTGATATGT TGAAGGGATT GATTATACCC AAGTATCAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262284.1
CDS	362..3343
Translation	MLTLYYVSATVLQSWRIQKACSKIAEHLAQPSSIAFYTLQSGSDDGFDPALASSELCCNRNAAKVTDILWLHANQRGCCLRPLQTLHITPWISFPPAPSLLPFSYAADTLTPASTPTEADVPRQQRVSLSAQGEERMQLLLEADIEPLQRPSSEDTSAVRLEDYAVQREGDPQPFELLAKHLKRQSRISVGLDKDGWKKHMEDLRAVGVLAHQATEFEYQRNRSEGRTKSDPTTHDQRPTSELLAKAVAVVEPTNKAYLSPARTTEASLKAEAKGTSTKPFPTKSDAKPATLAKSEGTPATSKEDSKLTPTKGALKTSELEKLAAVQAAQQQKKEAVQVSPVKAAFLAKPPMLSKHDEPDKASDQPTKPRKSFEAVKPLNSPPSSRAAPSRPVLQRNKDSLQDAKPLNVLVYSDSASARESTLATLQQLLERNVYTIYPLMPQQAAQKYWTEQTALLVVCGSVAHGIGQILVDYFLQGGKVLSLCSDILHFVLPNYRTAEVREHELVQFSYDKWQRVKMMHHIFCYQPSPVKKHFSTDSEESTKSHSRKPSMELKDLAGHSHNLDVHVLGTEETWNTPSLMLAKSLQSGGKAVFSQVHLEMNPSEFESDETKYSILKQNERTRLEIFADLLGKYLDVQVRGGDGVDQPQPGVVYKHAYFLGRHEAKFELLEKLRLRCSGSDNVIATPNLTMKFCGKDDKPPVANNNVLPILIHSCPDDFSTVDYFDNLKTEHIGRLVIYAPVVSSSMHLINNLELIHGLAVLPVQQTSGVGRRNNQWLSPPGCAMFSLQLHLTMDSALSSRLPLLQHLVGTAIVNSLRSHEEYGVLDISIKWPNDIYANGNQKIGGLVINTTLQGSQAIVNIGSGINLNNSRPTVCINDLIREYNTRVPNNKLPILKYELLIAMIFNEIERLLGEVQNGDFDSFYALYYSLWLHSGQSVKICLQKDQEKEAEIVGIDDFGFLEVKLPTGTIEIVQPDGNSFDMLKGLIIPKYQ

Target ORF information:

RefSeq Version	NM_001275355.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster holocarboxylase synthetase, transcript variant B (Hcs), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275355.1

ORF Insert Sequence:

ATGTTGACCC TGTATTACGT GAGTGCCACC GTCCTGCAGT CCTGGCGCAT TCAAAAGGCG 
TGCTCCAAGA TCGCCGAGCA CCTCGCGCAG CCCTCGAGCA TCGCCTTCTA TACCCTCCAA 
TCGGGCAGCG ATGACGGATT CGATCCCGCC CTGGCCAGCT CGGAGTTGTG CTGCAATCGG 
AACGCGGCCA AGGTCACGGA CATCCTGTGG CTGCACGCCA ACCAGCGGGG TTGCTGCCTG 
CGTCCGCTGC AAACGCTACA CATCACGCCT TGGATCAGCT TTCCGCCGGC GCCCAGTCTG 
CTTCCCTTCT CCTATGCCGC CGACACGTTA ACGCCGGCGT CCACGCCCAC GGAGGCGGAC 
GTCCCGCGCC AACAACGGGT TTCGCTCTCT GCCCAAGGGG AGGAGCGGAT GCAGCTGCTC 
CTTGAGGCCG ACATAGAGCC ACTACAGCGG CCCAGCTCCG AGGACACATC AGCAGTACGG 
CTGGAAGATT ACGCGGTGCA ACGGGAAGGG GATCCGCAGC CCTTTGAGCT TCTGGCCAAG 
CATCTGAAGC GGCAATCCCG CATAAGCGTC GGTCTGGACA AGGATGGATG GAAGAAGCAC 
ATGGAGGACT TGCGTGCCGT AGGTGTGCTG GCGCACCAGG CCACCGAGTT CGAGTACCAG 
AGGAATCGCT CTGAGGGAAG AACGAAATCG GATCCCACCA CACATGATCA ACGCCCGACT 
TCGGAGCTTT TGGCCAAGGC TGTGGCCGTG GTGGAGCCCA CCAACAAGGC ATATCTGTCT 
CCGGCAAGGA CGACAGAAGC CTCGCTCAAA GCCGAGGCCA AGGGAACGTC AACAAAACCT 
TTTCCGACTA AAAGCGATGC CAAACCCGCC ACTCTTGCCA AGAGCGAGGG CACGCCAGCC 
ACAAGCAAAG AGGACTCCAA GTTAACGCCA ACTAAGGGTG CATTAAAGAC AAGCGAACTA 
GAGAAACTAG CGGCTGTCCA GGCAGCGCAG CAGCAGAAGA AAGAGGCAGT CCAGGTCTCT 
CCCGTCAAGG CTGCTTTCTT AGCTAAACCA CCTATGCTAA GTAAACATGA TGAGCCTGAT 
AAGGCATCAG ATCAGCCAAC GAAGCCCAGG AAATCCTTTG AGGCGGTAAA GCCGTTAAAT 
TCTCCTCCGT CATCCAGAGC TGCTCCCTCG CGTCCTGTGC TGCAACGCAA CAAGGATAGC 
CTGCAGGACG CAAAACCATT GAATGTTTTG GTCTACTCAG ACAGCGCCAG TGCGCGGGAA 
TCCACATTGG CCACTCTGCA GCAGCTGCTG GAGCGCAATG TGTACACGAT CTATCCTCTG 
ATGCCTCAGC AAGCGGCTCA AAAATACTGG ACGGAACAGA CTGCGTTGCT GGTCGTCTGC 
GGATCGGTGG CACACGGAAT TGGGCAGATA TTGGTGGACT ACTTTCTGCA GGGCGGCAAG 
GTGTTAAGCC TGTGCTCGGA TATACTGCAC TTTGTACTCC CCAACTATCG CACAGCGGAG 
GTTCGAGAGC ACGAATTGGT TCAGTTCTCC TATGACAAGT GGCAGCGGGT CAAGATGATG 
CACCACATCT TTTGCTACCA ACCGTCGCCG GTGAAGAAGC ACTTCTCCAC TGACAGCGAG 
GAGTCCACCA AGTCGCACTC TCGCAAACCG TCCATGGAAC TGAAGGATCT GGCCGGTCAC 
AGCCACAATT TGGATGTCCA TGTGCTGGGA ACCGAGGAAA CCTGGAATAC GCCCAGCTTG 
ATGTTGGCCA AAAGCCTGCA GAGCGGCGGA AAGGCCGTCT TCTCCCAAGT GCACTTGGAA 
ATGAATCCCA GCGAATTTGA ATCCGATGAG ACTAAGTACT CCATTCTAAA GCAAAACGAG 
CGAACTCGCC TCGAGATATT TGCGGATCTC CTGGGAAAGT ACTTGGATGT GCAGGTCCGC 
GGTGGAGACG GTGTCGACCA GCCGCAGCCT GGAGTTGTCT ACAAGCACGC ATATTTCCTA 
GGTCGGCACG AGGCCAAGTT TGAGCTTCTG GAGAAGCTAC GTCTGCGCTG CAGCGGATCC 
GACAACGTGA TAGCAACGCC GAACCTGACG ATGAAGTTCT GTGGCAAGGA CGATAAGCCT 
CCAGTGGCCA ACAACAATGT CCTGCCCATA CTCATACATT CCTGTCCGGA TGACTTCTCC 
ACCGTGGATT ACTTCGATAA TCTTAAGACG GAACACATCG GACGACTGGT CATCTATGCA 
CCGGTGGTCA GCAGCTCCAT GCATCTCATA AACAACCTGG AGCTCATCCA CGGCCTGGCT 
GTGCTCCCCG TGCAGCAGAC GTCCGGAGTG GGTCGCAGAA ATAATCAATG GCTTAGTCCG 
CCGGGCTGTG CCATGTTCTC GCTGCAGCTG CACCTGACCA TGGACAGCGC TTTGAGCTCT 
CGGCTGCCTC TGTTGCAGCA CCTTGTTGGA ACGGCGATTG TTAATTCGCT CCGCAGTCAT 
GAAGAGTACG GGGTGCTGGA TATTTCCATT AAGTGGCCGA ATGACATTTA TGCAAATGGA 
AATCAGAAAA TTGGGGGCCT CGTCATTAAT ACCACACTTC AGGGATCCCA AGCCATCGTC 
AATATCGGAA GTGGAATTAA TTTGAACAAT TCAAGACCAA CAGTTTGCAT CAATGACTTG 
ATAAGAGAAT ATAATACCCG TGTCCCGAAC AACAAGTTAC CTATACTTAA GTACGAGCTA 
CTTATTGCCA TGATATTCAA TGAAATCGAA AGACTTTTGG GAGAAGTACA AAACGGAGAC 
TTTGATAGTT TTTATGCATT ATACTATTCG CTATGGTTAC ACAGCGGGCA ATCTGTTAAG 
ATTTGCCTAC AAAAAGATCA GGAAAAAGAG GCCGAAATTG TGGGAATCGA TGACTTTGGA 
TTTTTGGAGG TGAAATTACC AACTGGTACT ATAGAAATTG TGCAGCCCGA TGGAAACAGC 
TTTGATATGT TGAAGGGATT GATTATACCC AAGTATCAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hcs ( NM_001300247.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287176.1 Drosophila melanogaster holocarboxylase synthetase, transcript variant D (Hcs), mRNA.

Price & Availability↑

CloneID	OFa22051
Clone ID Related Accession (Same CDS sequence)	NM_001300247.1
Accession Version	NM_001300247.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	holocarboxylase synthetase, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGACCC TGTATTACGT GAGTGCCACC GTCCTGCAGT CCTGGCGCAT TCAAAAGGCG 
TGCTCCAAGA TCGCCGAGCA CCTCGCGCAG CCCTCGAGCA TCGCCTTCTA TACCCTCCAA 
TCGGGCAGCG ATGACGGATT CGATCCCGCC CTGGCCAGCT CGGAGTTGTG CTGCAATCGG 
AACGCGGCCA AGGTCACGGA CATCCTGTGG CTGCACGCCA ACCAGCGGGG TTGCTGCCTG 
CGTCCGCTGC AAACGCTACA CATCACGCCT TGGATCAGCT TTCCGCCGGC GCCCAGTCTG 
CTTCCCTTCT CCTATGCCGC CGACACGTTA ACGCCGGCGT CCACGCCCAC GGAGGCGGAC 
GTCCCGCGCC AACAACGGGT TTCGCTCTCT GCCCAAGGGG AGGAGCGGAT GCAGCTGCTC 
CTTGAGGCCG ACATAGAGCC ACTACAGCGG CCCAGCTCCG AGGACACATC AGCAGTACGG 
CTGGAAGATT ACGCGGTGCA ACGGGAAGGG GATCCGCAGC CCTTTGAGCT TCTGGCCAAG 
CATCTGAAGC GGCAATCCCG CATAAGCGTC GGTCTGGACA AGGATGGATG GAAGAAGCAC 
ATGGAGGACT TGCGTGCCGT AGGTGTGCTG GCGCACCAGG CCACCGAGTT CGAGTACCAG 
AGGAATCGCT CTGAGGGAAG AACGAAATCG GATCCCACCA CACATGATCA ACGCCCGACT 
TCGGAGCTTT TGGCCAAGGC TGTGGCCGTG GTGGAGCCCA CCAACAAGGC ATATCTGTCT 
CCGGCAAGGA CGACAGAAGC CTCGCTCAAA GCCGAGGCCA AGGGAACGTC AACAAAACCT 
TTTCCGACTA AAAGCGATGC CAAACCCGCC ACTCTTGCCA AGAGCGAGGG CACGCCAGCC 
ACAAGCAAAG AGGACTCCAA GTTAACGCCA ACTAAGGGTG CATTAAAGAC AAGCGAACTA 
GAGAAACTAG CGGCTGTCCA GGCAGCGCAG CAGCAGAAGA AAGAGGCAGT CCAGGTCTCT 
CCCGTCAAGG CTGCTTTCTT AGCTAAACCA CCTATGCTAA GTAAACATGA TGAGCCTGAT 
AAGGCATCAG ATCAGCCAAC GAAGCCCAGG AAATCCTTTG AGGCGGTAAA GCCGTTAAAT 
TCTCCTCCGT CATCCAGAGC TGCTCCCTCG CGTCCTGTGC TGCAACGCAA CAAGGATAGC 
CTGCAGGACG CAAAACCATT GAATGTTTTG GTCTACTCAG ACAGCGCCAG TGCGCGGGAA 
TCCACATTGG CCACTCTGCA GCAGCTGCTG GAGCGCAATG TGTACACGAT CTATCCTCTG 
ATGCCTCAGC AAGCGGCTCA AAAATACTGG ACGGAACAGA CTGCGTTGCT GGTCGTCTGC 
GGATCGGTGG CACACGGAAT TGGGCAGATA TTGGTGGACT ACTTTCTGCA GGGCGGCAAG 
GTGTTAAGCC TGTGCTCGGA TATACTGCAC TTTGTACTCC CCAACTATCG CACAGCGGAG 
GTTCGAGAGC ACGAATTGGT TCAGTTCTCC TATGACAAGT GGCAGCGGGT CAAGATGATG 
CACCACATCT TTTGCTACCA ACCGTCGCCG GTGAAGAAGC ACTTCTCCAC TGACAGCGAG 
GAGTCCACCA AGTCGCACTC TCGCAAACCA GCTCTAGTAT GTCCAAGGTC CATGGAACTG 
AAGGATCTGG CCGGTCACAG CCACAATTTG GATGTCCATG TGCTGGGAAC CGAGGAAACC 
TGGAATACGC CCAGCTTGAT GTTGGCCAAA AGCCTGCAGA GCGGCGGAAA GGCCGTCTTC 
TCCCAAGTGC ACTTGGAAAT GAATCCCAGC GAATTTGAAT CCGATGAGAC TAAGTACTCC 
ATTCTAAAGC AAAACGAGCG AACTCGCCTC GAGATATTTG CGGATCTCCT GGGAAAGTAC 
TTGGATGTGC AGGTCCGCGG TGGAGACGGT GTCGACCAGC CGCAGCCTGG AGTTGTCTAC 
AAGCACGCAT ATTTCCTAGG TCGGCACGAG GCCAAGTTTG AGCTTCTGGA GAAGCTACGT 
CTGCGCTGCA GCGGATCCGA CAACGTGATA GCAACGCCGA ACCTGACGAT GAAGTTCTGT 
GGCAAGGACG ATAAGCCTCC AGTGGCCAAC AACAATGTCC TGCCCATACT CATACATTCC 
TGTCCGGATG ACTTCTCCAC CGTGGATTAC TTCGATAATC TTAAGACGGA ACACATCGGA 
CGACTGGTCA TCTATGCACC GGTGGTCAGC AGCTCCATGC ATCTCATAAA CAACCTGGAG 
CTCATCCACG GCCTGGCTGT GCTCCCCGTG CAGCAGACGT CCGGAGTGGG TCGCAGAAAT 
AATCAATGGC TTAGTCCGCC GGGCTGTGCC ATGTTCTCGC TGCAGCTGCA CCTGACCATG 
GACAGCGCTT TGAGCTCTCG GCTGCCTCTG TTGCAGCACC TTGTTGGAAC GGCGATTGTT 
AATTCGCTCC GCAGTCATGA AGAGTACGGG GTGCTGGATA TTTCCATTAA GTGGCCGAAT 
GACATTTATG CAAATGGAAA TCAGAAAATT GGGGGCCTCG TCATTAATAC CACACTTCAG 
GGATCCCAAG CCATCGTCAA TATCGGAAGT GGAATTAATT TGAACAATTC AAGACCAACA 
GTTTGCATCA ATGACTTGAT AAGAGAATAT AATACCCGTG TCCCGAACAA CAAGTTACCT 
ATACTTAAGT ACGAGCTACT TATTGCCATG ATATTCAATG AAATCGAAAG ACTTTTGGGA 
GAAGTACAAA ACGGAGACTT TGATAGTTTT TATGCATTAT ACTATTCGCT ATGGTTACAC 
AGCGGGCAAT CTGTTAAGAT TTGCCTACAA AAAGATCAGG AAAAAGAGGC CGAAATTGTG 
GGAATCGATG ACTTTGGATT TTTGGAGGTG AAATTACCAA CTGGTACTAT AGAAATTGTG 
CAGCCCGATG GAAACAGCTT TGATATGTTG AAGGGATTGA TTATACCCAA GTATCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287176.1
CDS	362..3361
Translation	MLTLYYVSATVLQSWRIQKACSKIAEHLAQPSSIAFYTLQSGSDDGFDPALASSELCCNRNAAKVTDILWLHANQRGCCLRPLQTLHITPWISFPPAPSLLPFSYAADTLTPASTPTEADVPRQQRVSLSAQGEERMQLLLEADIEPLQRPSSEDTSAVRLEDYAVQREGDPQPFELLAKHLKRQSRISVGLDKDGWKKHMEDLRAVGVLAHQATEFEYQRNRSEGRTKSDPTTHDQRPTSELLAKAVAVVEPTNKAYLSPARTTEASLKAEAKGTSTKPFPTKSDAKPATLAKSEGTPATSKEDSKLTPTKGALKTSELEKLAAVQAAQQQKKEAVQVSPVKAAFLAKPPMLSKHDEPDKASDQPTKPRKSFEAVKPLNSPPSSRAAPSRPVLQRNKDSLQDAKPLNVLVYSDSASARESTLATLQQLLERNVYTIYPLMPQQAAQKYWTEQTALLVVCGSVAHGIGQILVDYFLQGGKVLSLCSDILHFVLPNYRTAEVREHELVQFSYDKWQRVKMMHHIFCYQPSPVKKHFSTDSEESTKSHSRKPALVCPRSMELKDLAGHSHNLDVHVLGTEETWNTPSLMLAKSLQSGGKAVFSQVHLEMNPSEFESDETKYSILKQNERTRLEIFADLLGKYLDVQVRGGDGVDQPQPGVVYKHAYFLGRHEAKFELLEKLRLRCSGSDNVIATPNLTMKFCGKDDKPPVANNNVLPILIHSCPDDFSTVDYFDNLKTEHIGRLVIYAPVVSSSMHLINNLELIHGLAVLPVQQTSGVGRRNNQWLSPPGCAMFSLQLHLTMDSALSSRLPLLQHLVGTAIVNSLRSHEEYGVLDISIKWPNDIYANGNQKIGGLVINTTLQGSQAIVNIGSGINLNNSRPTVCINDLIREYNTRVPNNKLPILKYELLIAMIFNEIERLLGEVQNGDFDSFYALYYSLWLHSGQSVKICLQKDQEKEAEIVGIDDFGFLEVKLPTGTIEIVQPDGNSFDMLKGLIIPKYQ

Target ORF information:

RefSeq Version	NM_001300247.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster holocarboxylase synthetase, transcript variant D (Hcs), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300247.1

ORF Insert Sequence:

ATGTTGACCC TGTATTACGT GAGTGCCACC GTCCTGCAGT CCTGGCGCAT TCAAAAGGCG 
TGCTCCAAGA TCGCCGAGCA CCTCGCGCAG CCCTCGAGCA TCGCCTTCTA TACCCTCCAA 
TCGGGCAGCG ATGACGGATT CGATCCCGCC CTGGCCAGCT CGGAGTTGTG CTGCAATCGG 
AACGCGGCCA AGGTCACGGA CATCCTGTGG CTGCACGCCA ACCAGCGGGG TTGCTGCCTG 
CGTCCGCTGC AAACGCTACA CATCACGCCT TGGATCAGCT TTCCGCCGGC GCCCAGTCTG 
CTTCCCTTCT CCTATGCCGC CGACACGTTA ACGCCGGCGT CCACGCCCAC GGAGGCGGAC 
GTCCCGCGCC AACAACGGGT TTCGCTCTCT GCCCAAGGGG AGGAGCGGAT GCAGCTGCTC 
CTTGAGGCCG ACATAGAGCC ACTACAGCGG CCCAGCTCCG AGGACACATC AGCAGTACGG 
CTGGAAGATT ACGCGGTGCA ACGGGAAGGG GATCCGCAGC CCTTTGAGCT TCTGGCCAAG 
CATCTGAAGC GGCAATCCCG CATAAGCGTC GGTCTGGACA AGGATGGATG GAAGAAGCAC 
ATGGAGGACT TGCGTGCCGT AGGTGTGCTG GCGCACCAGG CCACCGAGTT CGAGTACCAG 
AGGAATCGCT CTGAGGGAAG AACGAAATCG GATCCCACCA CACATGATCA ACGCCCGACT 
TCGGAGCTTT TGGCCAAGGC TGTGGCCGTG GTGGAGCCCA CCAACAAGGC ATATCTGTCT 
CCGGCAAGGA CGACAGAAGC CTCGCTCAAA GCCGAGGCCA AGGGAACGTC AACAAAACCT 
TTTCCGACTA AAAGCGATGC CAAACCCGCC ACTCTTGCCA AGAGCGAGGG CACGCCAGCC 
ACAAGCAAAG AGGACTCCAA GTTAACGCCA ACTAAGGGTG CATTAAAGAC AAGCGAACTA 
GAGAAACTAG CGGCTGTCCA GGCAGCGCAG CAGCAGAAGA AAGAGGCAGT CCAGGTCTCT 
CCCGTCAAGG CTGCTTTCTT AGCTAAACCA CCTATGCTAA GTAAACATGA TGAGCCTGAT 
AAGGCATCAG ATCAGCCAAC GAAGCCCAGG AAATCCTTTG AGGCGGTAAA GCCGTTAAAT 
TCTCCTCCGT CATCCAGAGC TGCTCCCTCG CGTCCTGTGC TGCAACGCAA CAAGGATAGC 
CTGCAGGACG CAAAACCATT GAATGTTTTG GTCTACTCAG ACAGCGCCAG TGCGCGGGAA 
TCCACATTGG CCACTCTGCA GCAGCTGCTG GAGCGCAATG TGTACACGAT CTATCCTCTG 
ATGCCTCAGC AAGCGGCTCA AAAATACTGG ACGGAACAGA CTGCGTTGCT GGTCGTCTGC 
GGATCGGTGG CACACGGAAT TGGGCAGATA TTGGTGGACT ACTTTCTGCA GGGCGGCAAG 
GTGTTAAGCC TGTGCTCGGA TATACTGCAC TTTGTACTCC CCAACTATCG CACAGCGGAG 
GTTCGAGAGC ACGAATTGGT TCAGTTCTCC TATGACAAGT GGCAGCGGGT CAAGATGATG 
CACCACATCT TTTGCTACCA ACCGTCGCCG GTGAAGAAGC ACTTCTCCAC TGACAGCGAG 
GAGTCCACCA AGTCGCACTC TCGCAAACCA GCTCTAGTAT GTCCAAGGTC CATGGAACTG 
AAGGATCTGG CCGGTCACAG CCACAATTTG GATGTCCATG TGCTGGGAAC CGAGGAAACC 
TGGAATACGC CCAGCTTGAT GTTGGCCAAA AGCCTGCAGA GCGGCGGAAA GGCCGTCTTC 
TCCCAAGTGC ACTTGGAAAT GAATCCCAGC GAATTTGAAT CCGATGAGAC TAAGTACTCC 
ATTCTAAAGC AAAACGAGCG AACTCGCCTC GAGATATTTG CGGATCTCCT GGGAAAGTAC 
TTGGATGTGC AGGTCCGCGG TGGAGACGGT GTCGACCAGC CGCAGCCTGG AGTTGTCTAC 
AAGCACGCAT ATTTCCTAGG TCGGCACGAG GCCAAGTTTG AGCTTCTGGA GAAGCTACGT 
CTGCGCTGCA GCGGATCCGA CAACGTGATA GCAACGCCGA ACCTGACGAT GAAGTTCTGT 
GGCAAGGACG ATAAGCCTCC AGTGGCCAAC AACAATGTCC TGCCCATACT CATACATTCC 
TGTCCGGATG ACTTCTCCAC CGTGGATTAC TTCGATAATC TTAAGACGGA ACACATCGGA 
CGACTGGTCA TCTATGCACC GGTGGTCAGC AGCTCCATGC ATCTCATAAA CAACCTGGAG 
CTCATCCACG GCCTGGCTGT GCTCCCCGTG CAGCAGACGT CCGGAGTGGG TCGCAGAAAT 
AATCAATGGC TTAGTCCGCC GGGCTGTGCC ATGTTCTCGC TGCAGCTGCA CCTGACCATG 
GACAGCGCTT TGAGCTCTCG GCTGCCTCTG TTGCAGCACC TTGTTGGAAC GGCGATTGTT 
AATTCGCTCC GCAGTCATGA AGAGTACGGG GTGCTGGATA TTTCCATTAA GTGGCCGAAT 
GACATTTATG CAAATGGAAA TCAGAAAATT GGGGGCCTCG TCATTAATAC CACACTTCAG 
GGATCCCAAG CCATCGTCAA TATCGGAAGT GGAATTAATT TGAACAATTC AAGACCAACA 
GTTTGCATCA ATGACTTGAT AAGAGAATAT AATACCCGTG TCCCGAACAA CAAGTTACCT 
ATACTTAAGT ACGAGCTACT TATTGCCATG ATATTCAATG AAATCGAAAG ACTTTTGGGA 
GAAGTACAAA ACGGAGACTT TGATAGTTTT TATGCATTAT ACTATTCGCT ATGGTTACAC 
AGCGGGCAAT CTGTTAAGAT TTGCCTACAA AAAGATCAGG AAAAAGAGGC CGAAATTGTG 
GGAATCGATG ACTTTGGATT TTTGGAGGTG AAATTACCAA CTGGTACTAT AGAAATTGTG 
CAGCCCGATG GAAACAGCTT TGATATGTTG AAGGGATTGA TTATACCCAA GTATCAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hcs ( NM_141285.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649542.1 Drosophila melanogaster holocarboxylase synthetase, transcript variant A (Hcs), mRNA.

Price & Availability↑

CloneID	OFa03934
Clone ID Related Accession (Same CDS sequence)	NM_141285.2
Accession Version	NM_141285.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	holocarboxylase synthetase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141285.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGACCC TGTATTACGT GAGTGCCACC GTCCTGCAGT CCTGGCGCAT TCAAAAGGCG 
TGCTCCAAGA TCGCCGAGCA CCTCGCGCAG CCCTCGAGCA TCGCCTTCTA TACCCTCCAA 
TCGGGCAGCG ATGACGGATT CGATCCCGCC CTGGCCAGCT CGGAGTTGTG CTGCAATCGG 
AACGCGGCCA AGGTCACGGA CATCCTGTGG CTGCACGCCA ACCAGCGGGG TTGCTGCCTG 
CGTCCGCTGC AAACGCTACA CATCACGCCT TGGATCAGCT TTCCGCCGGC GCCCAGTCTG 
CTTCCCTTCT CCTATGCCGC CGACACGTTA ACGCCGGCGT CCACGCCCAC GGAGGCGGAC 
GTCCCGCGCC AACAACGGGT TTCGCTCTCT GCCCAAGGGG AGGAGCGGAT GCAGCTGCTC 
CTTGAGGCCG ACATAGAGCC ACTACAGCGG CCCAGCTCCG AGGACACATC AGCAGTACGG 
CTGGAAGATT ACGGTAAGCT AATTGCTTGG AAAATAGATT CCCACCTGGC GGTGCTCATC 
GAAACAGACG TCGAGCACTT CACGAAACTT TTGATAACGA CTTTCTTGAG AAATAATTTA 
TGCATTAACG ATCAGCTGCC ACTTTTGCGG ATTGAGTCGG TGCAACGGGA AGGGGATCCG 
CAGCCCTTTG AGCTTCTGGC CAAGCATCTG AAGCGGCAAT CCCGCATAAG CGTCGGTCTG 
GACAAGGATG GATGGAAGAA GCACATGGAG GACTTGCGTG CCGTAGGTGT GCTGGCGCAC 
CAGGCCACCG AGTTCGAGTA CCAGAGGAAT CGCTCTGAGG GAAGAACGAA ATCGGATCCC 
ACCACACATG ATCAACGCCC GACTTCGGAG CTTTTGGCCA AGGCTGTGGC CGTGGTGGAG 
CCCACCAACA AGGCATATCT GTCTCCGGCA AGGACGACAG AAGCCTCGCT CAAAGCCGAG 
GCCAAGGGAA CGTCAACAAA ACCTTTTCCG ACTAAAAGCG ATGCCAAACC CGCCACTCTT 
GCCAAGAGCG AGGGCACGCC AGCCACAAGC AAAGAGGACT CCAAGTTAAC GCCAACTAAG 
GGTGCATTAA AGACAAGCGA ACTAGAGAAA CTAGCGGCTG TCCAGGCAGC GCAGCAGCAG 
AAGAAAGAGG CAGTCCAGGT CTCTCCCGTC AAGGCTGCTT TCTTAGCTAA ACCACCTATG 
CTAAGTAAAC ATGATGAGCC TGATAAGGCA TCAGATCAGC CAACGAAGCC CAGGAAATCC 
TTTGAGGCGG TAAAGCCGTT AAATTCTCCT CCGTCATCCA GAGCTGCTCC CTCGCGTCCT 
GTGCTGCAAC GCAACAAGGA TAGCCTGCAG GACGCAAAAC CATTGAATGT TTTGGTCTAC 
TCAGACAGCG CCAGTGCGCG GGAATCCACA TTGGCCACTC TGCAGCAGCT GCTGGAGCGC 
AATGTGTACA CGATCTATCC TCTGATGCCT CAGCAAGCGG CTCAAAAATA CTGGACGGAA 
CAGACTGCGT TGCTGGTCGT CTGCGGATCG GTGGCACACG GAATTGGGCA GATATTGGTG 
GACTACTTTC TGCAGGGCGG CAAGGTGTTA AGCCTGTGCT CGGATATACT GCACTTTGTA 
CTCCCCAACT ATCGCACAGC GGAGGTTCGA GAGCACGAAT TGGTTCAGTT CTCCTATGAC 
AAGTGGCAGC GGGTCAAGAT GATGCACCAC ATCTTTTGCT ACCAACCGTC GCCGGTGAAG 
AAGCACTTCT CCACTGACAG CGAGGAGTCC ACCAAGTCGC ACTCTCGCAA ACCGTCCATG 
GAACTGAAGG ATCTGGCCGG TCACAGCCAC AATTTGGATG TCCATGTGCT GGGAACCGAG 
GAAACCTGGA ATACGCCCAG CTTGATGTTG GCCAAAAGCC TGCAGAGCGG CGGAAAGGCC 
GTCTTCTCCC AAGTGCACTT GGAAATGAAT CCCAGCGAAT TTGAATCCGA TGAGACTAAG 
TACTCCATTC TAAAGCAAAA CGAGCGAACT CGCCTCGAGA TATTTGCGGA TCTCCTGGGA 
AAGTACTTGG ATGTGCAGGT CCGCGGTGGA GACGGTGTCG ACCAGCCGCA GCCTGGAGTT 
GTCTACAAGC ACGCATATTT CCTAGGTCGG CACGAGGCCA AGTTTGAGCT TCTGGAGAAG 
CTACGTCTGC GCTGCAGCGG ATCCGACAAC GTGATAGCAA CGCCGAACCT GACGATGAAG 
TTCTGTGGCA AGGACGATAA GCCTCCAGTG GCCAACAACA ATGTCCTGCC CATACTCATA 
CATTCCTGTC CGGATGACTT CTCCACCGTG GATTACTTCG ATAATCTTAA GACGGAACAC 
ATCGGACGAC TGGTCATCTA TGCACCGGTG GTCAGCAGCT CCATGCATCT CATAAACAAC 
CTGGAGCTCA TCCACGGCCT GGCTGTGCTC CCCGTGCAGC AGACGTCCGG AGTGGGTCGC 
AGAAATAATC AATGGCTTAG TCCGCCGGGC TGTGCCATGT TCTCGCTGCA GCTGCACCTG 
ACCATGGACA GCGCTTTGAG CTCTCGGCTG CCTCTGTTGC AGCACCTTGT TGGAACGGCG 
ATTGTTAATT CGCTCCGCAG TCATGAAGAG TACGGGGTGC TGGATATTTC CATTAAGTGG 
CCGAATGACA TTTATGCAAA TGGAAATCAG AAAATTGGGG GCCTCGTCAT TAATACCACA 
CTTCAGGGAT CCCAAGCCAT CGTCAATATC GGAAGTGGAA TTAATTTGAA CAATTCAAGA 
CCAACAGTTT GCATCAATGA CTTGATAAGA GAATATAATA CCCGTGTCCC GAACAACAAG 
TTACCTATAC TTAAGTACGA GCTACTTATT GCCATGATAT TCAATGAAAT CGAAAGACTT 
TTGGGAGAAG TACAAAACGG AGACTTTGAT AGTTTTTATG CATTATACTA TTCGCTATGG 
TTACACAGCG GGCAATCTGT TAAGATTTGC CTACAAAAAG ATCAGGAAAA AGAGGCCGAA 
ATTGTGGGAA TCGATGACTT TGGATTTTTG GAGGTGAAAT TACCAACTGG TACTATAGAA 
ATTGTGCAGC CCGATGGAAA CAGCTTTGAT ATGTTGAAGG GATTGATTAT ACCCAAGTAT 
CAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649542.1
CDS	362..3487
Translation	MLTLYYVSATVLQSWRIQKACSKIAEHLAQPSSIAFYTLQSGSDDGFDPALASSELCCNRNAAKVTDILWLHANQRGCCLRPLQTLHITPWISFPPAPSLLPFSYAADTLTPASTPTEADVPRQQRVSLSAQGEERMQLLLEADIEPLQRPSSEDTSAVRLEDYGKLIAWKIDSHLAVLIETDVEHFTKLLITTFLRNNLCINDQLPLLRIESVQREGDPQPFELLAKHLKRQSRISVGLDKDGWKKHMEDLRAVGVLAHQATEFEYQRNRSEGRTKSDPTTHDQRPTSELLAKAVAVVEPTNKAYLSPARTTEASLKAEAKGTSTKPFPTKSDAKPATLAKSEGTPATSKEDSKLTPTKGALKTSELEKLAAVQAAQQQKKEAVQVSPVKAAFLAKPPMLSKHDEPDKASDQPTKPRKSFEAVKPLNSPPSSRAAPSRPVLQRNKDSLQDAKPLNVLVYSDSASARESTLATLQQLLERNVYTIYPLMPQQAAQKYWTEQTALLVVCGSVAHGIGQILVDYFLQGGKVLSLCSDILHFVLPNYRTAEVREHELVQFSYDKWQRVKMMHHIFCYQPSPVKKHFSTDSEESTKSHSRKPSMELKDLAGHSHNLDVHVLGTEETWNTPSLMLAKSLQSGGKAVFSQVHLEMNPSEFESDETKYSILKQNERTRLEIFADLLGKYLDVQVRGGDGVDQPQPGVVYKHAYFLGRHEAKFELLEKLRLRCSGSDNVIATPNLTMKFCGKDDKPPVANNNVLPILIHSCPDDFSTVDYFDNLKTEHIGRLVIYAPVVSSSMHLINNLELIHGLAVLPVQQTSGVGRRNNQWLSPPGCAMFSLQLHLTMDSALSSRLPLLQHLVGTAIVNSLRSHEEYGVLDISIKWPNDIYANGNQKIGGLVINTTLQGSQAIVNIGSGINLNNSRPTVCINDLIREYNTRVPNNKLPILKYELLIAMIFNEIERLLGEVQNGDFDSFYALYYSLWLHSGQSVKICLQKDQEKEAEIVGIDDFGFLEVKLPTGTIEIVQPDGNSFDMLKGLIIPKYQ

Target ORF information:

RefSeq Version	NM_141285.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster holocarboxylase synthetase, transcript variant A (Hcs), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141285.2

ORF Insert Sequence:

ATGTTGACCC TGTATTACGT GAGTGCCACC GTCCTGCAGT CCTGGCGCAT TCAAAAGGCG 
TGCTCCAAGA TCGCCGAGCA CCTCGCGCAG CCCTCGAGCA TCGCCTTCTA TACCCTCCAA 
TCGGGCAGCG ATGACGGATT CGATCCCGCC CTGGCCAGCT CGGAGTTGTG CTGCAATCGG 
AACGCGGCCA AGGTCACGGA CATCCTGTGG CTGCACGCCA ACCAGCGGGG TTGCTGCCTG 
CGTCCGCTGC AAACGCTACA CATCACGCCT TGGATCAGCT TTCCGCCGGC GCCCAGTCTG 
CTTCCCTTCT CCTATGCCGC CGACACGTTA ACGCCGGCGT CCACGCCCAC GGAGGCGGAC 
GTCCCGCGCC AACAACGGGT TTCGCTCTCT GCCCAAGGGG AGGAGCGGAT GCAGCTGCTC 
CTTGAGGCCG ACATAGAGCC ACTACAGCGG CCCAGCTCCG AGGACACATC AGCAGTACGG 
CTGGAAGATT ACGGTAAGCT AATTGCTTGG AAAATAGATT CCCACCTGGC GGTGCTCATC 
GAAACAGACG TCGAGCACTT CACGAAACTT TTGATAACGA CTTTCTTGAG AAATAATTTA 
TGCATTAACG ATCAGCTGCC ACTTTTGCGG ATTGAGTCGG TGCAACGGGA AGGGGATCCG 
CAGCCCTTTG AGCTTCTGGC CAAGCATCTG AAGCGGCAAT CCCGCATAAG CGTCGGTCTG 
GACAAGGATG GATGGAAGAA GCACATGGAG GACTTGCGTG CCGTAGGTGT GCTGGCGCAC 
CAGGCCACCG AGTTCGAGTA CCAGAGGAAT CGCTCTGAGG GAAGAACGAA ATCGGATCCC 
ACCACACATG ATCAACGCCC GACTTCGGAG CTTTTGGCCA AGGCTGTGGC CGTGGTGGAG 
CCCACCAACA AGGCATATCT GTCTCCGGCA AGGACGACAG AAGCCTCGCT CAAAGCCGAG 
GCCAAGGGAA CGTCAACAAA ACCTTTTCCG ACTAAAAGCG ATGCCAAACC CGCCACTCTT 
GCCAAGAGCG AGGGCACGCC AGCCACAAGC AAAGAGGACT CCAAGTTAAC GCCAACTAAG 
GGTGCATTAA AGACAAGCGA ACTAGAGAAA CTAGCGGCTG TCCAGGCAGC GCAGCAGCAG 
AAGAAAGAGG CAGTCCAGGT CTCTCCCGTC AAGGCTGCTT TCTTAGCTAA ACCACCTATG 
CTAAGTAAAC ATGATGAGCC TGATAAGGCA TCAGATCAGC CAACGAAGCC CAGGAAATCC 
TTTGAGGCGG TAAAGCCGTT AAATTCTCCT CCGTCATCCA GAGCTGCTCC CTCGCGTCCT 
GTGCTGCAAC GCAACAAGGA TAGCCTGCAG GACGCAAAAC CATTGAATGT TTTGGTCTAC 
TCAGACAGCG CCAGTGCGCG GGAATCCACA TTGGCCACTC TGCAGCAGCT GCTGGAGCGC 
AATGTGTACA CGATCTATCC TCTGATGCCT CAGCAAGCGG CTCAAAAATA CTGGACGGAA 
CAGACTGCGT TGCTGGTCGT CTGCGGATCG GTGGCACACG GAATTGGGCA GATATTGGTG 
GACTACTTTC TGCAGGGCGG CAAGGTGTTA AGCCTGTGCT CGGATATACT GCACTTTGTA 
CTCCCCAACT ATCGCACAGC GGAGGTTCGA GAGCACGAAT TGGTTCAGTT CTCCTATGAC 
AAGTGGCAGC GGGTCAAGAT GATGCACCAC ATCTTTTGCT ACCAACCGTC GCCGGTGAAG 
AAGCACTTCT CCACTGACAG CGAGGAGTCC ACCAAGTCGC ACTCTCGCAA ACCGTCCATG 
GAACTGAAGG ATCTGGCCGG TCACAGCCAC AATTTGGATG TCCATGTGCT GGGAACCGAG 
GAAACCTGGA ATACGCCCAG CTTGATGTTG GCCAAAAGCC TGCAGAGCGG CGGAAAGGCC 
GTCTTCTCCC AAGTGCACTT GGAAATGAAT CCCAGCGAAT TTGAATCCGA TGAGACTAAG 
TACTCCATTC TAAAGCAAAA CGAGCGAACT CGCCTCGAGA TATTTGCGGA TCTCCTGGGA 
AAGTACTTGG ATGTGCAGGT CCGCGGTGGA GACGGTGTCG ACCAGCCGCA GCCTGGAGTT 
GTCTACAAGC ACGCATATTT CCTAGGTCGG CACGAGGCCA AGTTTGAGCT TCTGGAGAAG 
CTACGTCTGC GCTGCAGCGG ATCCGACAAC GTGATAGCAA CGCCGAACCT GACGATGAAG 
TTCTGTGGCA AGGACGATAA GCCTCCAGTG GCCAACAACA ATGTCCTGCC CATACTCATA 
CATTCCTGTC CGGATGACTT CTCCACCGTG GATTACTTCG ATAATCTTAA GACGGAACAC 
ATCGGACGAC TGGTCATCTA TGCACCGGTG GTCAGCAGCT CCATGCATCT CATAAACAAC 
CTGGAGCTCA TCCACGGCCT GGCTGTGCTC CCCGTGCAGC AGACGTCCGG AGTGGGTCGC 
AGAAATAATC AATGGCTTAG TCCGCCGGGC TGTGCCATGT TCTCGCTGCA GCTGCACCTG 
ACCATGGACA GCGCTTTGAG CTCTCGGCTG CCTCTGTTGC AGCACCTTGT TGGAACGGCG 
ATTGTTAATT CGCTCCGCAG TCATGAAGAG TACGGGGTGC TGGATATTTC CATTAAGTGG 
CCGAATGACA TTTATGCAAA TGGAAATCAG AAAATTGGGG GCCTCGTCAT TAATACCACA 
CTTCAGGGAT CCCAAGCCAT CGTCAATATC GGAAGTGGAA TTAATTTGAA CAATTCAAGA 
CCAACAGTTT GCATCAATGA CTTGATAAGA GAATATAATA CCCGTGTCCC GAACAACAAG 
TTACCTATAC TTAAGTACGA GCTACTTATT GCCATGATAT TCAATGAAAT CGAAAGACTT 
TTGGGAGAAG TACAAAACGG AGACTTTGAT AGTTTTTATG CATTATACTA TTCGCTATGG 
TTACACAGCG GGCAATCTGT TAAGATTTGC CTACAAAAAG ATCAGGAAAA AGAGGCCGAA 
ATTGTGGGAA TCGATGACTT TGGATTTTTG GAGGTGAAAT TACCAACTGG TACTATAGAA 
ATTGTGCAGC CCGATGGAAA CAGCTTTGAT ATGTTGAAGG GATTGATTAT ACCCAAGTAT 
CAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hcs ( NM_001300246.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287175.1 Drosophila melanogaster holocarboxylase synthetase, transcript variant C (Hcs), mRNA.

Price & Availability↑

CloneID	OFa22050
Clone ID Related Accession (Same CDS sequence)	NM_001300246.1
Accession Version	NM_001300246.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	holocarboxylase synthetase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGACCC TGTATTACGT GAGTGCCACC GTCCTGCAGT CCTGGCGCAT TCAAAAGGCG 
TGCTCCAAGA TCGCCGAGCA CCTCGCGCAG CCCTCGAGCA TCGCCTTCTA TACCCTCCAA 
TCGGGCAGCG ATGACGGATT CGATCCCGCC CTGGCCAGCT CGGAGTTGTG CTGCAATCGG 
AACGCGGCCA AGGTCACGGA CATCCTGTGG CTGCACGCCA ACCAGCGGGG TTGCTGCCTG 
CGTCCGCTGC AAACGCTACA CATCACGCCT TGGATCAGCT TTCCGCCGGC GCCCAGTCTG 
CTTCCCTTCT CCTATGCCGC CGACACGTTA ACGCCGGCGT CCACGCCCAC GGAGGCGGAC 
GTCCCGCGCC AACAACGGGT TTCGCTCTCT GCCCAAGGGG AGGAGCGGAT GCAGCTGCTC 
CTTGAGGCCG ACATAGAGCC ACTACAGCGG CCCAGCTCCG AGGACACATC AGCAGTACGG 
CTGGAAGATT ACGGTAAGCT AATTGCTTGG AAAATAGATT CCCACCTGGC GGTGCTCATC 
GAAACAGACG TCGAGCACTT CACGAAACTT TTGATAACGA CTTTCTTGAG AAATAATTTA 
TGCATTAACG ATCAGCTGCC ACTTTTGCGG ATTGAGTCGG TGCAACGGGA AGGGGATCCG 
CAGCCCTTTG AGCTTCTGGC CAAGCATCTG AAGCGGCAAT CCCGCATAAG CGTCGGTCTG 
GACAAGGATG GATGGAAGAA GCACATGGAG GACTTGCGTG CCGTAGGTGT GCTGGCGCAC 
CAGGCCACCG AGTTCGAGTA CCAGAGGAAT CGCTCTGAGG GAAGAACGAA ATCGGATCCC 
ACCACACATG ATCAACGCCC GACTTCGGAG CTTTTGGCCA AGGCTGTGGC CGTGGTGGAG 
CCCACCAACA AGGCATATCT GTCTCCGGCA AGGACGACAG AAGCCTCGCT CAAAGCCGAG 
GCCAAGGGAA CGTCAACAAA ACCTTTTCCG ACTAAAAGCG ATGCCAAACC CGCCACTCTT 
GCCAAGAGCG AGGGCACGCC AGCCACAAGC AAAGAGGACT CCAAGTTAAC GCCAACTAAG 
GGTGCATTAA AGACAAGCGA ACTAGAGAAA CTAGCGGCTG TCCAGGCAGC GCAGCAGCAG 
AAGAAAGAGG CAGTCCAGGT CTCTCCCGTC AAGGCTGCTT TCTTAGCTAA ACCACCTATG 
CTAAGTAAAC ATGATGAGCC TGATAAGGCA TCAGATCAGC CAACGAAGCC CAGGAAATCC 
TTTGAGGCGG TAAAGCCGTT AAATTCTCCT CCGTCATCCA GAGCTGCTCC CTCGCGTCCT 
GTGCTGCAAC GCAACAAGGA TAGCCTGCAG GACGCAAAAC CATTGAATGT TTTGGTCTAC 
TCAGACAGCG CCAGTGCGCG GGAATCCACA TTGGCCACTC TGCAGCAGCT GCTGGAGCGC 
AATGTGTACA CGATCTATCC TCTGATGCCT CAGCAAGCGG CTCAAAAATA CTGGACGGAA 
CAGACTGCGT TGCTGGTCGT CTGCGGATCG GTGGCACACG GAATTGGGCA GATATTGGTG 
GACTACTTTC TGCAGGGCGG CAAGGTGTTA AGCCTGTGCT CGGATATACT GCACTTTGTA 
CTCCCCAACT ATCGCACAGC GGAGGTTCGA GAGCACGAAT TGGTTCAGTT CTCCTATGAC 
AAGTGGCAGC GGGTCAAGAT GATGCACCAC ATCTTTTGCT ACCAACCGTC GCCGGTGAAG 
AAGCACTTCT CCACTGACAG CGAGGAGTCC ACCAAGTCGC ACTCTCGCAA ACCAGCTCTA 
GTATGTCCAA GGTCCATGGA ACTGAAGGAT CTGGCCGGTC ACAGCCACAA TTTGGATGTC 
CATGTGCTGG GAACCGAGGA AACCTGGAAT ACGCCCAGCT TGATGTTGGC CAAAAGCCTG 
CAGAGCGGCG GAAAGGCCGT CTTCTCCCAA GTGCACTTGG AAATGAATCC CAGCGAATTT 
GAATCCGATG AGACTAAGTA CTCCATTCTA AAGCAAAACG AGCGAACTCG CCTCGAGATA 
TTTGCGGATC TCCTGGGAAA GTACTTGGAT GTGCAGGTCC GCGGTGGAGA CGGTGTCGAC 
CAGCCGCAGC CTGGAGTTGT CTACAAGCAC GCATATTTCC TAGGTCGGCA CGAGGCCAAG 
TTTGAGCTTC TGGAGAAGCT ACGTCTGCGC TGCAGCGGAT CCGACAACGT GATAGCAACG 
CCGAACCTGA CGATGAAGTT CTGTGGCAAG GACGATAAGC CTCCAGTGGC CAACAACAAT 
GTCCTGCCCA TACTCATACA TTCCTGTCCG GATGACTTCT CCACCGTGGA TTACTTCGAT 
AATCTTAAGA CGGAACACAT CGGACGACTG GTCATCTATG CACCGGTGGT CAGCAGCTCC 
ATGCATCTCA TAAACAACCT GGAGCTCATC CACGGCCTGG CTGTGCTCCC CGTGCAGCAG 
ACGTCCGGAG TGGGTCGCAG AAATAATCAA TGGCTTAGTC CGCCGGGCTG TGCCATGTTC 
TCGCTGCAGC TGCACCTGAC CATGGACAGC GCTTTGAGCT CTCGGCTGCC TCTGTTGCAG 
CACCTTGTTG GAACGGCGAT TGTTAATTCG CTCCGCAGTC ATGAAGAGTA CGGGGTGCTG 
GATATTTCCA TTAAGTGGCC GAATGACATT TATGCAAATG GAAATCAGAA AATTGGGGGC 
CTCGTCATTA ATACCACACT TCAGGGATCC CAAGCCATCG TCAATATCGG AAGTGGAATT 
AATTTGAACA ATTCAAGACC AACAGTTTGC ATCAATGACT TGATAAGAGA ATATAATACC 
CGTGTCCCGA ACAACAAGTT ACCTATACTT AAGTACGAGC TACTTATTGC CATGATATTC 
AATGAAATCG AAAGACTTTT GGGAGAAGTA CAAAACGGAG ACTTTGATAG TTTTTATGCA 
TTATACTATT CGCTATGGTT ACACAGCGGG CAATCTGTTA AGATTTGCCT ACAAAAAGAT 
CAGGAAAAAG AGGCCGAAAT TGTGGGAATC GATGACTTTG GATTTTTGGA GGTGAAATTA 
CCAACTGGTA CTATAGAAAT TGTGCAGCCC GATGGAAACA GCTTTGATAT GTTGAAGGGA 
TTGATTATAC CCAAGTATCA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287175.1
CDS	362..3505
Translation	MLTLYYVSATVLQSWRIQKACSKIAEHLAQPSSIAFYTLQSGSDDGFDPALASSELCCNRNAAKVTDILWLHANQRGCCLRPLQTLHITPWISFPPAPSLLPFSYAADTLTPASTPTEADVPRQQRVSLSAQGEERMQLLLEADIEPLQRPSSEDTSAVRLEDYGKLIAWKIDSHLAVLIETDVEHFTKLLITTFLRNNLCINDQLPLLRIESVQREGDPQPFELLAKHLKRQSRISVGLDKDGWKKHMEDLRAVGVLAHQATEFEYQRNRSEGRTKSDPTTHDQRPTSELLAKAVAVVEPTNKAYLSPARTTEASLKAEAKGTSTKPFPTKSDAKPATLAKSEGTPATSKEDSKLTPTKGALKTSELEKLAAVQAAQQQKKEAVQVSPVKAAFLAKPPMLSKHDEPDKASDQPTKPRKSFEAVKPLNSPPSSRAAPSRPVLQRNKDSLQDAKPLNVLVYSDSASARESTLATLQQLLERNVYTIYPLMPQQAAQKYWTEQTALLVVCGSVAHGIGQILVDYFLQGGKVLSLCSDILHFVLPNYRTAEVREHELVQFSYDKWQRVKMMHHIFCYQPSPVKKHFSTDSEESTKSHSRKPALVCPRSMELKDLAGHSHNLDVHVLGTEETWNTPSLMLAKSLQSGGKAVFSQVHLEMNPSEFESDETKYSILKQNERTRLEIFADLLGKYLDVQVRGGDGVDQPQPGVVYKHAYFLGRHEAKFELLEKLRLRCSGSDNVIATPNLTMKFCGKDDKPPVANNNVLPILIHSCPDDFSTVDYFDNLKTEHIGRLVIYAPVVSSSMHLINNLELIHGLAVLPVQQTSGVGRRNNQWLSPPGCAMFSLQLHLTMDSALSSRLPLLQHLVGTAIVNSLRSHEEYGVLDISIKWPNDIYANGNQKIGGLVINTTLQGSQAIVNIGSGINLNNSRPTVCINDLIREYNTRVPNNKLPILKYELLIAMIFNEIERLLGEVQNGDFDSFYALYYSLWLHSGQSVKICLQKDQEKEAEIVGIDDFGFLEVKLPTGTIEIVQPDGNSFDMLKGLIIPKYQ

Target ORF information:

RefSeq Version	NM_001300246.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster holocarboxylase synthetase, transcript variant C (Hcs), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300246.1

ORF Insert Sequence:

ATGTTGACCC TGTATTACGT GAGTGCCACC GTCCTGCAGT CCTGGCGCAT TCAAAAGGCG 
TGCTCCAAGA TCGCCGAGCA CCTCGCGCAG CCCTCGAGCA TCGCCTTCTA TACCCTCCAA 
TCGGGCAGCG ATGACGGATT CGATCCCGCC CTGGCCAGCT CGGAGTTGTG CTGCAATCGG 
AACGCGGCCA AGGTCACGGA CATCCTGTGG CTGCACGCCA ACCAGCGGGG TTGCTGCCTG 
CGTCCGCTGC AAACGCTACA CATCACGCCT TGGATCAGCT TTCCGCCGGC GCCCAGTCTG 
CTTCCCTTCT CCTATGCCGC CGACACGTTA ACGCCGGCGT CCACGCCCAC GGAGGCGGAC 
GTCCCGCGCC AACAACGGGT TTCGCTCTCT GCCCAAGGGG AGGAGCGGAT GCAGCTGCTC 
CTTGAGGCCG ACATAGAGCC ACTACAGCGG CCCAGCTCCG AGGACACATC AGCAGTACGG 
CTGGAAGATT ACGGTAAGCT AATTGCTTGG AAAATAGATT CCCACCTGGC GGTGCTCATC 
GAAACAGACG TCGAGCACTT CACGAAACTT TTGATAACGA CTTTCTTGAG AAATAATTTA 
TGCATTAACG ATCAGCTGCC ACTTTTGCGG ATTGAGTCGG TGCAACGGGA AGGGGATCCG 
CAGCCCTTTG AGCTTCTGGC CAAGCATCTG AAGCGGCAAT CCCGCATAAG CGTCGGTCTG 
GACAAGGATG GATGGAAGAA GCACATGGAG GACTTGCGTG CCGTAGGTGT GCTGGCGCAC 
CAGGCCACCG AGTTCGAGTA CCAGAGGAAT CGCTCTGAGG GAAGAACGAA ATCGGATCCC 
ACCACACATG ATCAACGCCC GACTTCGGAG CTTTTGGCCA AGGCTGTGGC CGTGGTGGAG 
CCCACCAACA AGGCATATCT GTCTCCGGCA AGGACGACAG AAGCCTCGCT CAAAGCCGAG 
GCCAAGGGAA CGTCAACAAA ACCTTTTCCG ACTAAAAGCG ATGCCAAACC CGCCACTCTT 
GCCAAGAGCG AGGGCACGCC AGCCACAAGC AAAGAGGACT CCAAGTTAAC GCCAACTAAG 
GGTGCATTAA AGACAAGCGA ACTAGAGAAA CTAGCGGCTG TCCAGGCAGC GCAGCAGCAG 
AAGAAAGAGG CAGTCCAGGT CTCTCCCGTC AAGGCTGCTT TCTTAGCTAA ACCACCTATG 
CTAAGTAAAC ATGATGAGCC TGATAAGGCA TCAGATCAGC CAACGAAGCC CAGGAAATCC 
TTTGAGGCGG TAAAGCCGTT AAATTCTCCT CCGTCATCCA GAGCTGCTCC CTCGCGTCCT 
GTGCTGCAAC GCAACAAGGA TAGCCTGCAG GACGCAAAAC CATTGAATGT TTTGGTCTAC 
TCAGACAGCG CCAGTGCGCG GGAATCCACA TTGGCCACTC TGCAGCAGCT GCTGGAGCGC 
AATGTGTACA CGATCTATCC TCTGATGCCT CAGCAAGCGG CTCAAAAATA CTGGACGGAA 
CAGACTGCGT TGCTGGTCGT CTGCGGATCG GTGGCACACG GAATTGGGCA GATATTGGTG 
GACTACTTTC TGCAGGGCGG CAAGGTGTTA AGCCTGTGCT CGGATATACT GCACTTTGTA 
CTCCCCAACT ATCGCACAGC GGAGGTTCGA GAGCACGAAT TGGTTCAGTT CTCCTATGAC 
AAGTGGCAGC GGGTCAAGAT GATGCACCAC ATCTTTTGCT ACCAACCGTC GCCGGTGAAG 
AAGCACTTCT CCACTGACAG CGAGGAGTCC ACCAAGTCGC ACTCTCGCAA ACCAGCTCTA 
GTATGTCCAA GGTCCATGGA ACTGAAGGAT CTGGCCGGTC ACAGCCACAA TTTGGATGTC 
CATGTGCTGG GAACCGAGGA AACCTGGAAT ACGCCCAGCT TGATGTTGGC CAAAAGCCTG 
CAGAGCGGCG GAAAGGCCGT CTTCTCCCAA GTGCACTTGG AAATGAATCC CAGCGAATTT 
GAATCCGATG AGACTAAGTA CTCCATTCTA AAGCAAAACG AGCGAACTCG CCTCGAGATA 
TTTGCGGATC TCCTGGGAAA GTACTTGGAT GTGCAGGTCC GCGGTGGAGA CGGTGTCGAC 
CAGCCGCAGC CTGGAGTTGT CTACAAGCAC GCATATTTCC TAGGTCGGCA CGAGGCCAAG 
TTTGAGCTTC TGGAGAAGCT ACGTCTGCGC TGCAGCGGAT CCGACAACGT GATAGCAACG 
CCGAACCTGA CGATGAAGTT CTGTGGCAAG GACGATAAGC CTCCAGTGGC CAACAACAAT 
GTCCTGCCCA TACTCATACA TTCCTGTCCG GATGACTTCT CCACCGTGGA TTACTTCGAT 
AATCTTAAGA CGGAACACAT CGGACGACTG GTCATCTATG CACCGGTGGT CAGCAGCTCC 
ATGCATCTCA TAAACAACCT GGAGCTCATC CACGGCCTGG CTGTGCTCCC CGTGCAGCAG 
ACGTCCGGAG TGGGTCGCAG AAATAATCAA TGGCTTAGTC CGCCGGGCTG TGCCATGTTC 
TCGCTGCAGC TGCACCTGAC CATGGACAGC GCTTTGAGCT CTCGGCTGCC TCTGTTGCAG 
CACCTTGTTG GAACGGCGAT TGTTAATTCG CTCCGCAGTC ATGAAGAGTA CGGGGTGCTG 
GATATTTCCA TTAAGTGGCC GAATGACATT TATGCAAATG GAAATCAGAA AATTGGGGGC 
CTCGTCATTA ATACCACACT TCAGGGATCC CAAGCCATCG TCAATATCGG AAGTGGAATT 
AATTTGAACA ATTCAAGACC AACAGTTTGC ATCAATGACT TGATAAGAGA ATATAATACC 
CGTGTCCCGA ACAACAAGTT ACCTATACTT AAGTACGAGC TACTTATTGC CATGATATTC 
AATGAAATCG AAAGACTTTT GGGAGAAGTA CAAAACGGAG ACTTTGATAG TTTTTATGCA 
TTATACTATT CGCTATGGTT ACACAGCGGG CAATCTGTTA AGATTTGCCT ACAAAAAGAT 
CAGGAAAAAG AGGCCGAAAT TGTGGGAATC GATGACTTTG GATTTTTGGA GGTGAAATTA 
CCAACTGGTA CTATAGAAAT TGTGCAGCCC GATGGAAACA GCTTTGATAT GTTGAAGGGA 
TTGATTATAC CCAAGTATCA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Hcs cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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