CG12974 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG12974
Entrez Gene ID	40350
Full Name	CG12974 gene product from transcript CG12974-RD
Synonyms	Dmel\CG12974
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 78C4-78C5

mRNA and Protein(s)

mRNA	Protein	Name
NM_141039.1	NP_649296.1	uncharacterized protein, isoform A
NM_168895.3	NP_730613.2	uncharacterized protein, isoform B
NM_001275213.1	NP_001262142.1	uncharacterized protein, isoform C
NM_001300210.1	NP_001287139.1	uncharacterized protein, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG12974 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG12974 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19189	NM_001275213.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG12974), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa19189	NM_141039.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG12974), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa20301	NM_168895.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG12974), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa20301	NM_001300210.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant D (CG12974), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG12974 ( NM_141039.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649296.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG12974), mRNA.

Price & Availability↑

CloneID	OFa19189
Clone ID Related Accession (Same CDS sequence)	NM_141039.1 , NM_001275213.1
Accession Version	NM_141039.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCTTGA CCATAACAGC GATATGTGCC TACAATTGTG TCCTGTATAT TACAAATGAC 
CTCACCTATG GCTCCATGGT CAAGACTGTA TTCTTTGACG TGTACTTCAA GGGCAGTTGC 
AAGATGAATC CCAGTGATTA CCAGAACTAC GACCAAACCA TCCTGAATAC AGTGACTACT 
GTTTTTGATC CCACCCAGAT TTTGATCTGG AATTCGGTCT ACCTGGGCGT TTCGGTTTGC 
TGGCTAATCG TCTCCGTTGT GCTGCTACTG TGGGTGCGCA AGGACAACCC GAAGCAGACC 
GTGACTGCCA TCTACATCTG GGCCTTCTTC GTGGCCACCA TATGCTGCAT GGATGTAGCT 
TCTGGAGTGA TCTTCGGCGT GGACTACGGA CGTTTCCACA AGGAGGCTCT GAGGTATAAT 
GCCAATAGTA TCAATGCTGG TGTCATCTAT CCCTTGGCTG CCACCTTGAT CGCCGGAGGA 
GTGGCTGCCA TTTCCATGAT GGTCATCTCC TTCAAGGGAT TCGTTCTGTG GTTCATTAAC 
TTTGGACTGC TCGCATACCT TGTCGCGAGA GCCATTCGAA TTTCCACCGA TAAGGACAAT 
GTGGATACTT TGTTCAATCC TCGCAAGGAT TCCAATGATA TTCTGACCAC CAGGACCCCG 
ATTCGCGCCT ACGAGGAGGT GGAGGTCCAG GCGTATAACA ATGCCGCCTA CGTTCCGGAA 
ACCCGTTCCA TGGCCGAGAC CATCCAGGTG AACGAGGGAG CCATCGTGCG GGCGGCGCAC 
TTGTCGATGG ACTCGAACCT AATGGATCGT CGCTTCAGGG ACCTCAACGC GTACCAGCAG 
TACCCGCCTC CGAATCCGCA GCAAAGTCGC CCGCTGAAGC AATCTTCGCA GAATCCCATG 
GAGACCATTG TGGTGGCCAC CTCGGGATTC CCCGTGCCGG ACTACTCCCC GCAGCCCAGT 
CCCAATGGCG TTCTGCGCCA CAACCAGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649296.1
CDS	261..1253
Translation	MGLTITAICAYNCVLYITNDLTYGSMVKTVFFDVYFKGSCKMNPSDYQNYDQTILNTVTTVFDPTQILIWNSVYLGVSVCWLIVSVVLLLWVRKDNPKQTVTAIYIWAFFVATICCMDVASGVIFGVDYGRFHKEALRYNANSINAGVIYPLAATLIAGGVAAISMMVISFKGFVLWFINFGLLAYLVARAIRISTDKDNVDTLFNPRKDSNDILTTRTPIRAYEEVEVQAYNNAAYVPETRSMAETIQVNEGAIVRAAHLSMDSNLMDRRFRDLNAYQQYPPPNPQQSRPLKQSSQNPMETIVVATSGFPVPDYSPQPSPNGVLRHNQY

Target ORF information:

RefSeq Version	NM_141039.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG12974), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141039.1

ORF Insert Sequence:

ATGGGCTTGA CCATAACAGC GATATGTGCC TACAATTGTG TCCTGTATAT TACAAATGAC 
CTCACCTATG GCTCCATGGT CAAGACTGTA TTCTTTGACG TGTACTTCAA GGGCAGTTGC 
AAGATGAATC CCAGTGATTA CCAGAACTAC GACCAAACCA TCCTGAATAC AGTGACTACT 
GTTTTTGATC CCACCCAGAT TTTGATCTGG AATTCGGTCT ACCTGGGCGT TTCGGTTTGC 
TGGCTAATCG TCTCCGTTGT GCTGCTACTG TGGGTGCGCA AGGACAACCC GAAGCAGACC 
GTGACTGCCA TCTACATCTG GGCCTTCTTC GTGGCCACCA TATGCTGCAT GGATGTAGCT 
TCTGGAGTGA TCTTCGGCGT GGACTACGGA CGTTTCCACA AGGAGGCTCT GAGGTATAAT 
GCCAATAGTA TCAATGCTGG TGTCATCTAT CCCTTGGCTG CCACCTTGAT CGCCGGAGGA 
GTGGCTGCCA TTTCCATGAT GGTCATCTCC TTCAAGGGAT TCGTTCTGTG GTTCATTAAC 
TTTGGACTGC TCGCATACCT TGTCGCGAGA GCCATTCGAA TTTCCACCGA TAAGGACAAT 
GTGGATACTT TGTTCAATCC TCGCAAGGAT TCCAATGATA TTCTGACCAC CAGGACCCCG 
ATTCGCGCCT ACGAGGAGGT GGAGGTCCAG GCGTATAACA ATGCCGCCTA CGTTCCGGAA 
ACCCGTTCCA TGGCCGAGAC CATCCAGGTG AACGAGGGAG CCATCGTGCG GGCGGCGCAC 
TTGTCGATGG ACTCGAACCT AATGGATCGT CGCTTCAGGG ACCTCAACGC GTACCAGCAG 
TACCCGCCTC CGAATCCGCA GCAAAGTCGC CCGCTGAAGC AATCTTCGCA GAATCCCATG 
GAGACCATTG TGGTGGCCAC CTCGGGATTC CCCGTGCCGG ACTACTCCCC GCAGCCCAGT 
CCCAATGGCG TTCTGCGCCA CAACCAGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG12974 ( NM_001275213.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262142.1 Drosophila melanogaster uncharacterized protein, transcript variant C (CG12974), mRNA.

Price & Availability↑

CloneID	OFa19189
Clone ID Related Accession (Same CDS sequence)	NM_141039.1 , NM_001275213.1
Accession Version	NM_001275213.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCTTGA CCATAACAGC GATATGTGCC TACAATTGTG TCCTGTATAT TACAAATGAC 
CTCACCTATG GCTCCATGGT CAAGACTGTA TTCTTTGACG TGTACTTCAA GGGCAGTTGC 
AAGATGAATC CCAGTGATTA CCAGAACTAC GACCAAACCA TCCTGAATAC AGTGACTACT 
GTTTTTGATC CCACCCAGAT TTTGATCTGG AATTCGGTCT ACCTGGGCGT TTCGGTTTGC 
TGGCTAATCG TCTCCGTTGT GCTGCTACTG TGGGTGCGCA AGGACAACCC GAAGCAGACC 
GTGACTGCCA TCTACATCTG GGCCTTCTTC GTGGCCACCA TATGCTGCAT GGATGTAGCT 
TCTGGAGTGA TCTTCGGCGT GGACTACGGA CGTTTCCACA AGGAGGCTCT GAGGTATAAT 
GCCAATAGTA TCAATGCTGG TGTCATCTAT CCCTTGGCTG CCACCTTGAT CGCCGGAGGA 
GTGGCTGCCA TTTCCATGAT GGTCATCTCC TTCAAGGGAT TCGTTCTGTG GTTCATTAAC 
TTTGGACTGC TCGCATACCT TGTCGCGAGA GCCATTCGAA TTTCCACCGA TAAGGACAAT 
GTGGATACTT TGTTCAATCC TCGCAAGGAT TCCAATGATA TTCTGACCAC CAGGACCCCG 
ATTCGCGCCT ACGAGGAGGT GGAGGTCCAG GCGTATAACA ATGCCGCCTA CGTTCCGGAA 
ACCCGTTCCA TGGCCGAGAC CATCCAGGTG AACGAGGGAG CCATCGTGCG GGCGGCGCAC 
TTGTCGATGG ACTCGAACCT AATGGATCGT CGCTTCAGGG ACCTCAACGC GTACCAGCAG 
TACCCGCCTC CGAATCCGCA GCAAAGTCGC CCGCTGAAGC AATCTTCGCA GAATCCCATG 
GAGACCATTG TGGTGGCCAC CTCGGGATTC CCCGTGCCGG ACTACTCCCC GCAGCCCAGT 
CCCAATGGCG TTCTGCGCCA CAACCAGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262142.1
CDS	767..1759
Translation	MGLTITAICAYNCVLYITNDLTYGSMVKTVFFDVYFKGSCKMNPSDYQNYDQTILNTVTTVFDPTQILIWNSVYLGVSVCWLIVSVVLLLWVRKDNPKQTVTAIYIWAFFVATICCMDVASGVIFGVDYGRFHKEALRYNANSINAGVIYPLAATLIAGGVAAISMMVISFKGFVLWFINFGLLAYLVARAIRISTDKDNVDTLFNPRKDSNDILTTRTPIRAYEEVEVQAYNNAAYVPETRSMAETIQVNEGAIVRAAHLSMDSNLMDRRFRDLNAYQQYPPPNPQQSRPLKQSSQNPMETIVVATSGFPVPDYSPQPSPNGVLRHNQY

Target ORF information:

RefSeq Version	NM_001275213.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG12974), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275213.1

ORF Insert Sequence:

ATGGGCTTGA CCATAACAGC GATATGTGCC TACAATTGTG TCCTGTATAT TACAAATGAC 
CTCACCTATG GCTCCATGGT CAAGACTGTA TTCTTTGACG TGTACTTCAA GGGCAGTTGC 
AAGATGAATC CCAGTGATTA CCAGAACTAC GACCAAACCA TCCTGAATAC AGTGACTACT 
GTTTTTGATC CCACCCAGAT TTTGATCTGG AATTCGGTCT ACCTGGGCGT TTCGGTTTGC 
TGGCTAATCG TCTCCGTTGT GCTGCTACTG TGGGTGCGCA AGGACAACCC GAAGCAGACC 
GTGACTGCCA TCTACATCTG GGCCTTCTTC GTGGCCACCA TATGCTGCAT GGATGTAGCT 
TCTGGAGTGA TCTTCGGCGT GGACTACGGA CGTTTCCACA AGGAGGCTCT GAGGTATAAT 
GCCAATAGTA TCAATGCTGG TGTCATCTAT CCCTTGGCTG CCACCTTGAT CGCCGGAGGA 
GTGGCTGCCA TTTCCATGAT GGTCATCTCC TTCAAGGGAT TCGTTCTGTG GTTCATTAAC 
TTTGGACTGC TCGCATACCT TGTCGCGAGA GCCATTCGAA TTTCCACCGA TAAGGACAAT 
GTGGATACTT TGTTCAATCC TCGCAAGGAT TCCAATGATA TTCTGACCAC CAGGACCCCG 
ATTCGCGCCT ACGAGGAGGT GGAGGTCCAG GCGTATAACA ATGCCGCCTA CGTTCCGGAA 
ACCCGTTCCA TGGCCGAGAC CATCCAGGTG AACGAGGGAG CCATCGTGCG GGCGGCGCAC 
TTGTCGATGG ACTCGAACCT AATGGATCGT CGCTTCAGGG ACCTCAACGC GTACCAGCAG 
TACCCGCCTC CGAATCCGCA GCAAAGTCGC CCGCTGAAGC AATCTTCGCA GAATCCCATG 
GAGACCATTG TGGTGGCCAC CTCGGGATTC CCCGTGCCGG ACTACTCCCC GCAGCCCAGT 
CCCAATGGCG TTCTGCGCCA CAACCAGTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG12974 ( NM_168895.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730613.2 Drosophila melanogaster uncharacterized protein, transcript variant B (CG12974), mRNA.

Price & Availability↑

CloneID	OFa20301
Clone ID Related Accession (Same CDS sequence)	NM_168895.3 , NM_001300210.1
Accession Version	NM_168895.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168895.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTATTCAG CACCAGCGGC AAAAATCAAG CACTTTGGCT CCCTTAAAAC GGTAGCAGCT 
TGGGCCGGAA TTCTCGGAGT GGCCCAGTCC ATCATCTGGA TGGGCTTGAC CATAACAGCG 
ATATGTGCCT ACAATTGTGT CCTGTATATT ACAAATGACC TCACCTATGG CTCCATGGTC 
AAGACTGTAT TCTTTGACGT GTACTTCAAG GGCAGTTGCA AGATGAATCC CAGTGATTAC 
CAGAACTACG ACCAAACCAT CCTGAATACA GTGACTACTG TTTTTGATCC CACCCAGATT 
TTGATCTGGA ATTCGGTCTA CCTGGGCGTT TCGGTTTGCT GGCTAATCGT CTCCGTTGTG 
CTGCTACTGT GGGTGCGCAA GGACAACCCG AAGCAGACCG TGACTGCCAT CTACATCTGG 
GCCTTCTTCG TGGCCACCAT ATGCTGCATG GATGTAGCTT CTGGAGTGAT CTTCGGCGTG 
GACTACGGAC GTTTCCACAA GGAGGCTCTG AGGTATAATG CCAATAGTAT CAATGCTGGT 
GTCATCTATC CCTTGGCTGC CACCTTGATC GCCGGAGGAG TGGCTGCCAT TTCCATGATG 
GTCATCTCCT TCAAGGGATT CGTTCTGTGG TTCATTAACT TTGGACTGCT CGCATACCTT 
GTCGCGAGAG CCATTCGAAT TTCCACCGAT AAGGACAATG TGGATACTTT GTTCAATCCT 
CGCAAGGATT CCAATGATAT TCTGACCACC AGGACCCCGA TTCGCGCCTA CGAGGAGGTG 
GAGGTCCAGG CGTATAACAA TGCCGCCTAC GTTCCGGAAA CCCGTTCCAT GGCCGAGACC 
ATCCAGGTGA ACGAGGGAGC CATCGTGCGG GCGGCGCACT TGTCGATGGA CTCGAACCTA 
ATGGATCGTC GCTTCAGGGA CCTCAACGCG TACCAGCAGT ACCCGCCTCC GAATCCGCAG 
CAAAGTCGCC CGCTGAAGCA ATCTTCGCAG AATCCCATGG AGACCATTGT GGTGGCCACC 
TCGGGATTCC CCGTGCCGGA CTACTCCCCG CAGCCCAGTC CCAATGGCGT TCTGCGCCAC 
AACCAGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730613.2
CDS	302..1393
Translation	MYSAPAAKIKHFGSLKTVAAWAGILGVAQSIIWMGLTITAICAYNCVLYITNDLTYGSMVKTVFFDVYFKGSCKMNPSDYQNYDQTILNTVTTVFDPTQILIWNSVYLGVSVCWLIVSVVLLLWVRKDNPKQTVTAIYIWAFFVATICCMDVASGVIFGVDYGRFHKEALRYNANSINAGVIYPLAATLIAGGVAAISMMVISFKGFVLWFINFGLLAYLVARAIRISTDKDNVDTLFNPRKDSNDILTTRTPIRAYEEVEVQAYNNAAYVPETRSMAETIQVNEGAIVRAAHLSMDSNLMDRRFRDLNAYQQYPPPNPQQSRPLKQSSQNPMETIVVATSGFPVPDYSPQPSPNGVLRHNQY

Target ORF information:

RefSeq Version	NM_168895.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG12974), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168895.3

ORF Insert Sequence:

ATGTATTCAG CACCAGCGGC AAAAATCAAG CACTTTGGCT CCCTTAAAAC GGTAGCAGCT 
TGGGCCGGAA TTCTCGGAGT GGCCCAGTCC ATCATCTGGA TGGGCTTGAC CATAACAGCG 
ATATGTGCCT ACAATTGTGT CCTGTATATT ACAAATGACC TCACCTATGG CTCCATGGTC 
AAGACTGTAT TCTTTGACGT GTACTTCAAG GGCAGTTGCA AGATGAATCC CAGTGATTAC 
CAGAACTACG ACCAAACCAT CCTGAATACA GTGACTACTG TTTTTGATCC CACCCAGATT 
TTGATCTGGA ATTCGGTCTA CCTGGGCGTT TCGGTTTGCT GGCTAATCGT CTCCGTTGTG 
CTGCTACTGT GGGTGCGCAA GGACAACCCG AAGCAGACCG TGACTGCCAT CTACATCTGG 
GCCTTCTTCG TGGCCACCAT ATGCTGCATG GATGTAGCTT CTGGAGTGAT CTTCGGCGTG 
GACTACGGAC GTTTCCACAA GGAGGCTCTG AGGTATAATG CCAATAGTAT CAATGCTGGT 
GTCATCTATC CCTTGGCTGC CACCTTGATC GCCGGAGGAG TGGCTGCCAT TTCCATGATG 
GTCATCTCCT TCAAGGGATT CGTTCTGTGG TTCATTAACT TTGGACTGCT CGCATACCTT 
GTCGCGAGAG CCATTCGAAT TTCCACCGAT AAGGACAATG TGGATACTTT GTTCAATCCT 
CGCAAGGATT CCAATGATAT TCTGACCACC AGGACCCCGA TTCGCGCCTA CGAGGAGGTG 
GAGGTCCAGG CGTATAACAA TGCCGCCTAC GTTCCGGAAA CCCGTTCCAT GGCCGAGACC 
ATCCAGGTGA ACGAGGGAGC CATCGTGCGG GCGGCGCACT TGTCGATGGA CTCGAACCTA 
ATGGATCGTC GCTTCAGGGA CCTCAACGCG TACCAGCAGT ACCCGCCTCC GAATCCGCAG 
CAAAGTCGCC CGCTGAAGCA ATCTTCGCAG AATCCCATGG AGACCATTGT GGTGGCCACC 
TCGGGATTCC CCGTGCCGGA CTACTCCCCG CAGCCCAGTC CCAATGGCGT TCTGCGCCAC 
AACCAGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG12974 ( NM_001300210.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287139.1 Drosophila melanogaster uncharacterized protein, transcript variant D (CG12974), mRNA.

Price & Availability↑

CloneID	OFa20301
Clone ID Related Accession (Same CDS sequence)	NM_168895.3 , NM_001300210.1
Accession Version	NM_001300210.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTATTCAG CACCAGCGGC AAAAATCAAG CACTTTGGCT CCCTTAAAAC GGTAGCAGCT 
TGGGCCGGAA TTCTCGGAGT GGCCCAGTCC ATCATCTGGA TGGGCTTGAC CATAACAGCG 
ATATGTGCCT ACAATTGTGT CCTGTATATT ACAAATGACC TCACCTATGG CTCCATGGTC 
AAGACTGTAT TCTTTGACGT GTACTTCAAG GGCAGTTGCA AGATGAATCC CAGTGATTAC 
CAGAACTACG ACCAAACCAT CCTGAATACA GTGACTACTG TTTTTGATCC CACCCAGATT 
TTGATCTGGA ATTCGGTCTA CCTGGGCGTT TCGGTTTGCT GGCTAATCGT CTCCGTTGTG 
CTGCTACTGT GGGTGCGCAA GGACAACCCG AAGCAGACCG TGACTGCCAT CTACATCTGG 
GCCTTCTTCG TGGCCACCAT ATGCTGCATG GATGTAGCTT CTGGAGTGAT CTTCGGCGTG 
GACTACGGAC GTTTCCACAA GGAGGCTCTG AGGTATAATG CCAATAGTAT CAATGCTGGT 
GTCATCTATC CCTTGGCTGC CACCTTGATC GCCGGAGGAG TGGCTGCCAT TTCCATGATG 
GTCATCTCCT TCAAGGGATT CGTTCTGTGG TTCATTAACT TTGGACTGCT CGCATACCTT 
GTCGCGAGAG CCATTCGAAT TTCCACCGAT AAGGACAATG TGGATACTTT GTTCAATCCT 
CGCAAGGATT CCAATGATAT TCTGACCACC AGGACCCCGA TTCGCGCCTA CGAGGAGGTG 
GAGGTCCAGG CGTATAACAA TGCCGCCTAC GTTCCGGAAA CCCGTTCCAT GGCCGAGACC 
ATCCAGGTGA ACGAGGGAGC CATCGTGCGG GCGGCGCACT TGTCGATGGA CTCGAACCTA 
ATGGATCGTC GCTTCAGGGA CCTCAACGCG TACCAGCAGT ACCCGCCTCC GAATCCGCAG 
CAAAGTCGCC CGCTGAAGCA ATCTTCGCAG AATCCCATGG AGACCATTGT GGTGGCCACC 
TCGGGATTCC CCGTGCCGGA CTACTCCCCG CAGCCCAGTC CCAATGGCGT TCTGCGCCAC 
AACCAGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287139.1
CDS	302..1393
Translation	MYSAPAAKIKHFGSLKTVAAWAGILGVAQSIIWMGLTITAICAYNCVLYITNDLTYGSMVKTVFFDVYFKGSCKMNPSDYQNYDQTILNTVTTVFDPTQILIWNSVYLGVSVCWLIVSVVLLLWVRKDNPKQTVTAIYIWAFFVATICCMDVASGVIFGVDYGRFHKEALRYNANSINAGVIYPLAATLIAGGVAAISMMVISFKGFVLWFINFGLLAYLVARAIRISTDKDNVDTLFNPRKDSNDILTTRTPIRAYEEVEVQAYNNAAYVPETRSMAETIQVNEGAIVRAAHLSMDSNLMDRRFRDLNAYQQYPPPNPQQSRPLKQSSQNPMETIVVATSGFPVPDYSPQPSPNGVLRHNQY

Target ORF information:

RefSeq Version	NM_001300210.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant D (CG12974), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300210.1

ORF Insert Sequence:

ATGTATTCAG CACCAGCGGC AAAAATCAAG CACTTTGGCT CCCTTAAAAC GGTAGCAGCT 
TGGGCCGGAA TTCTCGGAGT GGCCCAGTCC ATCATCTGGA TGGGCTTGAC CATAACAGCG 
ATATGTGCCT ACAATTGTGT CCTGTATATT ACAAATGACC TCACCTATGG CTCCATGGTC 
AAGACTGTAT TCTTTGACGT GTACTTCAAG GGCAGTTGCA AGATGAATCC CAGTGATTAC 
CAGAACTACG ACCAAACCAT CCTGAATACA GTGACTACTG TTTTTGATCC CACCCAGATT 
TTGATCTGGA ATTCGGTCTA CCTGGGCGTT TCGGTTTGCT GGCTAATCGT CTCCGTTGTG 
CTGCTACTGT GGGTGCGCAA GGACAACCCG AAGCAGACCG TGACTGCCAT CTACATCTGG 
GCCTTCTTCG TGGCCACCAT ATGCTGCATG GATGTAGCTT CTGGAGTGAT CTTCGGCGTG 
GACTACGGAC GTTTCCACAA GGAGGCTCTG AGGTATAATG CCAATAGTAT CAATGCTGGT 
GTCATCTATC CCTTGGCTGC CACCTTGATC GCCGGAGGAG TGGCTGCCAT TTCCATGATG 
GTCATCTCCT TCAAGGGATT CGTTCTGTGG TTCATTAACT TTGGACTGCT CGCATACCTT 
GTCGCGAGAG CCATTCGAAT TTCCACCGAT AAGGACAATG TGGATACTTT GTTCAATCCT 
CGCAAGGATT CCAATGATAT TCTGACCACC AGGACCCCGA TTCGCGCCTA CGAGGAGGTG 
GAGGTCCAGG CGTATAACAA TGCCGCCTAC GTTCCGGAAA CCCGTTCCAT GGCCGAGACC 
ATCCAGGTGA ACGAGGGAGC CATCGTGCGG GCGGCGCACT TGTCGATGGA CTCGAACCTA 
ATGGATCGTC GCTTCAGGGA CCTCAACGCG TACCAGCAGT ACCCGCCTCC GAATCCGCAG 
CAAAGTCGCC CGCTGAAGCA ATCTTCGCAG AATCCCATGG AGACCATTGT GGTGGCCACC 
TCGGGATTCC CCGTGCCGGA CTACTCCCCG CAGCCCAGTC CCAATGGCGT TCTGCGCCAC 
AACCAGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG12974 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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