siz cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	siz
Entrez Gene ID	40327
Full Name	schizo
Synonyms	CG10577,CG12980,CG12981,CG32434,Dmel\CG32434,Loner,loner
General protein information	Preferred Names schizo Names CG32434 gene product from transcript CG32434-RB CG32434-PA CG32434-PB CG32434-PC CG32434-PD dBRAG loner siz-PA siz-PB siz-PC siz-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 78A5-78B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001043153.2	NP_001036618.1	schizo, isoform C
NM_001170016.1	NP_001163487.1	schizo, isoform D
NM_168881.3	NP_730594.1	schizo, isoform B
NM_206407.2	NP_996129.1	schizo, isoform A

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	siz	NP_730594.1
Caenorhabditis elegans (roundworm)	M02B7.5	NP_500420.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results suggest that N-cadherin is regulated in a Schizo/Loner- and d-Arf1-dependent manner.
Title: The Arf-GEF Schizo/Loner regulates N-cadherin to induce fusion competence of Drosophila myoblasts.

Results suggest an additive function of the intracellular domain of Duf and an early function of Rols and Loner which is independent of Duf.
Title: The intracellular domain of Dumbfounded affects myoblast fusion efficiency and interacts with Rolling pebbles and Loner.

The following siz gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the siz cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19178	NM_001170016.1 Latest version!	Drosophila melanogaster schizo (siz), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa21165	NM_168881.3 Latest version!	Drosophila melanogaster schizo (siz), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa21167	NM_206407.2 Latest version!	Drosophila melanogaster schizo (siz), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa21168	NM_001043153.2 Latest version!	Drosophila melanogaster schizo (siz), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

siz ( NM_001170016.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163487.1 Drosophila melanogaster schizo (siz), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa19178
Clone ID Related Accession (Same CDS sequence)	NM_001170016.1
Accession Version	NM_001170016.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	schizo, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCAGCT ACACTGCCGC CCAGTATTAC AAGACCACCA ATATGATTAC CTCCAACGAG 
ATTTACTGCT TTCCCCAGAA GAGCCTGGAG CGAAGTGGTT CCACCCAGTA TGAGCTGGCT 
GGAGCGCAAC AGCCAGGATC AGCCAACGCC TCCACCTGCA CAGATAGCGG CAGTGTTGGC 
GGCTATGTTT ACCTGCAGAA TCACTACGCC CCCGGCGCCC ACAGCTCCTC AGCCGCCATC 
AACTACCCTG CGCAACAGCA TCCCCAGATG GTCTACCAAA TACAGCAGTA TCCCACGTGC 
CATCAGCAGC AGCAACAGCA GCACCTCCAG CAGCAGCAGC ATTTGCATCA GACCGGCGCA 
GGTCACTACA TGCAGGTGAC GGCGACGGGT GGTGGCCAGT ATCATCACCA TCACATGCTC 
CATGGTCACG GGCATCATGC CCACCATCAC GGTGGGGCGG TGGTCATCGC CGGGAGTGGT 
GTGGGAACTG GCCTGGGATC TGGAGCCACC AGCGTGATCA TGCAGCACCA GCAACAGCAG 
CAACAGCAAC AGAATATGCA CAAGAAGAAC TCCATCCGGA ACGGCGGAGA TGTCCTCAAG 
CGAACGCGAG CTCAGTCGGC CTACGAACTC TCACAAGATC TGCTTGAGAA ACAGATCGAG 
CTGCTGGAGC GCAAGTACGG CGGAGTGCGA GCCCGTAACG CAGCGGTCAC TATTCAGCGC 
GCCTTCCGTC ACTACATGAT GGTCAAGAAG TTCGCCTCGA TCACGGCAAT GGCCAAAGCT 
GAGAAGCGTC TAAGCCGGCG GATGGTGGTT ACAGCCAGCA GTCTTGGATT GGCGGAGGAG 
GGTGCCTCCT CCTCGTCAGC CTACGGAAGT GCCACGGAAT CTCAGCTCAC CGAGCAGCAA 
CAGCAACAAC AGGCGCAGCA GCAACAGCAG CCACGTGTCA CCATCATGGC GGGTCCGGCG 
GGAGCAGCTT CTCCGGGCTT ATCCCGGACG CCACCTACGC GATCGCTTTC CATGCGGGAG 
CGACGTCAGC TGGACTGCAG TCCCATACCG CGTAGTCAGT CAGGAGCCTC TCCCGCCTCC 
ATATCGAGCT CGACAGTCAG CACATCGGCT CTGGCCTCAC ATCCACATGT TAATCTGCTG 
CACGCGGCAG AGCCACATTA TTATAATGCT CAGGCACTGC CCCAGGGAGC TGCTTACTAC 
ACTAGTTACC ATGGATCACC GCACGACTTG AGCTATGCCA GTTCGGCGGA CACCTCGCTA 
AATGCCTCGT GGGTTAACAC GAGCGGCCAC TCCCCGCACA CGCCCTACTA TTCGGCGGCC 
CAGATCTATA TGCGACCCAA GGGCGGCAGC ACCACGCCCA CGCCCAGTTG CAGTGGCAGC 
ACAGGTAGCG GCAGCGGAGG CAGCGGGAGT GGCAGTAGCA AGAAGGTGCC ACCAGAGGTG 
CCCAAACGCA CCAGTTCCAT TACGGCACAA CAGCAGACAC AGCTTCTTTT GCTGCAGCGC 
CAGACACCGC CGCCTCCTTC GCTGCTAAGG ACCAATGGCC TGTGCAAAAC CGCCGAGAAC 
GGCAGTCTGA CCTCCGTGCA GAGTTCCGGA TCGGATTCTA GTGTTACCTC TGCGGAACGC 
AATCTAAACA GCGATTTGGG CTCGGATCGC AGTAACTCAC CACATACATG GAAACGAGGA 
ACAGCCCTAA ATAGTTCCCA GCAGTTCTCC ACGCACTCGG CGGATTCAGC GGGTGCGGTG 
TCTGGTGGAG GAGTTGGAGT GGCTGGAGGA GCCGGCGTTT ATGCCGCTCA AATGCAGGCT 
GCCGTTGCAG CAGCTACAGC GGCAGGAGGA ATGCCACCAG CGGATGACCA TGCTATCTCC 
TCGCATACGA GTGCCGCTCA GTATGAGCAG CATGAGCAGC AGCAGCACGA GCAACAGCAA 
TTGCAGGCGG CAGCTGCAGC TGCCGGAGTG GCACAAAACT ACAAGATGTC GGAGACGATA 
CGCAAGCGAC AGTATCGCGT TGGACTCAAT CTGTTCAACA AGAAGCCGGA GAAGGGCATC 
ACCTATCTGA TCAGGCGGGG ATTCCTTGAG AATACACCAC AGGGCGTTGC TCGTTTCCTC 
ATCACCCGTA AGGGTTTGTC CCGGCAAATG ATTGGCGAGT ATCTGGGAAA TTTGCAGAAC 
CAGTTCAACA TGGCCGTGCT CAGTTGCTTT GCCATGGAGC TGGACCTGTC CGGCCGGCAA 
GTGGATGTGG CTTTGCGAAA GTTCCAGGCC TATTTCCGCA TGCCTGGAGA GGCACAAAAG 
ATTGAGCGCC TCATGGAGAT CTTTTCACAG CGCTATTGTG AATGCAATGC GGACATTGTC 
GGGCGATTGA GATCATCCGA TACGATCTTC GTCCTGGCTT TCGCCATCAT CATGCTGAAC 
ACGGATCTGC ACACACCCAA TCTAAAGCCA GAACGTCGCA TGCGCGTCGA GGACTTTATC 
AAGAATCTGC GCGGCATCGA CGACTGTCAC GACATTGATA AGGACATGCT GATGGGCATC 
TATGACCGTG TCAAGTCCGA CGAATTTAAG CCCGGCAGCG ACCATGTCAC TCAAGTGATG 
AAGGTACAGG CCACTATTGT GGGCAAGAAA CCAAATCTAG CGCTGCCCCA TCGGCGTCTT 
GTCTGCTATT GCCGACTGTA CGAGATTCCT GACGTGAACA AGAAGGAGCG ACCTGGTGTG 
CATCAGCGCG AGGTGTTTCT GTTCAACGAT CTGCTGGTCA TTACCAAAAT ATTTAGCAAA 
AAGAAGACCT CCGTGACGTA CACATTCCGC AACAGTTTCC CGCTATGCGG CACCGTTGTC 
ACCCTTCTGG ACATGCCCAA CTATCCGTTT TGCATTCAGC TCTCCCAGAA AGTGGATGGC 
AAGATCTTGA TCACCTTCAA CGCCCGCAAC GAACACGATC GCTGCAAGTT TGCCGAGGAT 
CTTAAAGAGT CCATTAGCGA GATGGACGAG ATGGAGTCGC TGCGCATTGA GGCCGAACTG 
GAGCGCCAGA AGTCGGCGCG CAATCGAGCA CCTGGCAATG CGGAGAATCG TGACAGTGGC 
GTAGCCGATG TGGAGGTCTG CCCATGCCCG TATCAGCAAG GATCACAAGC ATCTGGCGAG 
CAGGCTCCAA ACTCTGCCGA TAACTCGCAG CAGCTGAAGC GCAGTGCGCT GAGCAACAGC 
CTTCTCGACA TGCACGAGCA GTTTGGCAAT GAGAAACCTC AACGTCGTGG CAGCGTTGGC 
TCCCTGGACA GCGGCATGAG CATCTCGTTC CAGTCCACTA CAACATCCAG CGCTTCGAGG 
GAAAATGCCG CTGCCATTGC GGCCGCAGCA AATGCAGCTG CAGCAGCCAA GATGCGATTT 
AACATGCCGC CAACGGCAGC GATTGCCACG CCCAGCAATG TGTATGCAGC TCCGGGAATG 
CAGGCGTATA CCCATGCCAA CTTTGTGCAG CAGTCACAGG CCGCCTACAT GTTGCAGCAA 
CAGCAAATGC TCCAGCAGCA GGCACAAATG CAAGCTCAGG CTCAGGCTCA AGCCCAAGCT 
CAGGCCCAAG CTCAAGCCCA GGCTCAGGCG CAGGCGCAGG CGCAGCCGCT TACTGGCCGA 
ATACCGGGAC GCGAGCGAAA GGCTTCGCGG ACGGATGAGA ACGGACGGTC GACGGAGGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163487.1
CDS	501..4163
Translation	MSSYTAAQYYKTTNMITSNEIYCFPQKSLERSGSTQYELAGAQQPGSANASTCTDSGSVGGYVYLQNHYAPGAHSSSAAINYPAQQHPQMVYQIQQYPTCHQQQQQQHLQQQQHLHQTGAGHYMQVTATGGGQYHHHHMLHGHGHHAHHHGGAVVIAGSGVGTGLGSGATSVIMQHQQQQQQQQNMHKKNSIRNGGDVLKRTRAQSAYELSQDLLEKQIELLERKYGGVRARNAAVTIQRAFRHYMMVKKFASITAMAKAEKRLSRRMVVTASSLGLAEEGASSSSAYGSATESQLTEQQQQQQAQQQQQPRVTIMAGPAGAASPGLSRTPPTRSLSMRERRQLDCSPIPRSQSGASPASISSSTVSTSALASHPHVNLLHAAEPHYYNAQALPQGAAYYTSYHGSPHDLSYASSADTSLNASWVNTSGHSPHTPYYSAAQIYMRPKGGSTTPTPSCSGSTGSGSGGSGSGSSKKVPPEVPKRTSSITAQQQTQLLLLQRQTPPPPSLLRTNGLCKTAENGSLTSVQSSGSDSSVTSAERNLNSDLGSDRSNSPHTWKRGTALNSSQQFSTHSADSAGAVSGGGVGVAGGAGVYAAQMQAAVAAATAAGGMPPADDHAISSHTSAAQYEQHEQQQHEQQQLQAAAAAAGVAQNYKMSETIRKRQYRVGLNLFNKKPEKGITYLIRRGFLENTPQGVARFLITRKGLSRQMIGEYLGNLQNQFNMAVLSCFAMELDLSGRQVDVALRKFQAYFRMPGEAQKIERLMEIFSQRYCECNADIVGRLRSSDTIFVLAFAIIMLNTDLHTPNLKPERRMRVEDFIKNLRGIDDCHDIDKDMLMGIYDRVKSDEFKPGSDHVTQVMKVQATIVGKKPNLALPHRRLVCYCRLYEIPDVNKKERPGVHQREVFLFNDLLVITKIFSKKKTSVTYTFRNSFPLCGTVVTLLDMPNYPFCIQLSQKVDGKILITFNARNEHDRCKFAEDLKESISEMDEMESLRIEAELERQKSARNRAPGNAENRDSGVADVEVCPCPYQQGSQASGEQAPNSADNSQQLKRSALSNSLLDMHEQFGNEKPQRRGSVGSLDSGMSISFQSTTTSSASRENAAAIAAAANAAAAAKMRFNMPPTAAIATPSNVYAAPGMQAYTHANFVQQSQAAYMLQQQQMLQQQAQMQAQAQAQAQAQAQAQAQAQAQAQAQPLTGRIPGRERKASRTDENGRSTEV

Target ORF information:

RefSeq Version	NM_001170016.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster schizo (siz), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170016.1

ORF Insert Sequence:

ATGAGCAGCT ACACTGCCGC CCAGTATTAC AAGACCACCA ATATGATTAC CTCCAACGAG 
ATTTACTGCT TTCCCCAGAA GAGCCTGGAG CGAAGTGGTT CCACCCAGTA TGAGCTGGCT 
GGAGCGCAAC AGCCAGGATC AGCCAACGCC TCCACCTGCA CAGATAGCGG CAGTGTTGGC 
GGCTATGTTT ACCTGCAGAA TCACTACGCC CCCGGCGCCC ACAGCTCCTC AGCCGCCATC 
AACTACCCTG CGCAACAGCA TCCCCAGATG GTCTACCAAA TACAGCAGTA TCCCACGTGC 
CATCAGCAGC AGCAACAGCA GCACCTCCAG CAGCAGCAGC ATTTGCATCA GACCGGCGCA 
GGTCACTACA TGCAGGTGAC GGCGACGGGT GGTGGCCAGT ATCATCACCA TCACATGCTC 
CATGGTCACG GGCATCATGC CCACCATCAC GGTGGGGCGG TGGTCATCGC CGGGAGTGGT 
GTGGGAACTG GCCTGGGATC TGGAGCCACC AGCGTGATCA TGCAGCACCA GCAACAGCAG 
CAACAGCAAC AGAATATGCA CAAGAAGAAC TCCATCCGGA ACGGCGGAGA TGTCCTCAAG 
CGAACGCGAG CTCAGTCGGC CTACGAACTC TCACAAGATC TGCTTGAGAA ACAGATCGAG 
CTGCTGGAGC GCAAGTACGG CGGAGTGCGA GCCCGTAACG CAGCGGTCAC TATTCAGCGC 
GCCTTCCGTC ACTACATGAT GGTCAAGAAG TTCGCCTCGA TCACGGCAAT GGCCAAAGCT 
GAGAAGCGTC TAAGCCGGCG GATGGTGGTT ACAGCCAGCA GTCTTGGATT GGCGGAGGAG 
GGTGCCTCCT CCTCGTCAGC CTACGGAAGT GCCACGGAAT CTCAGCTCAC CGAGCAGCAA 
CAGCAACAAC AGGCGCAGCA GCAACAGCAG CCACGTGTCA CCATCATGGC GGGTCCGGCG 
GGAGCAGCTT CTCCGGGCTT ATCCCGGACG CCACCTACGC GATCGCTTTC CATGCGGGAG 
CGACGTCAGC TGGACTGCAG TCCCATACCG CGTAGTCAGT CAGGAGCCTC TCCCGCCTCC 
ATATCGAGCT CGACAGTCAG CACATCGGCT CTGGCCTCAC ATCCACATGT TAATCTGCTG 
CACGCGGCAG AGCCACATTA TTATAATGCT CAGGCACTGC CCCAGGGAGC TGCTTACTAC 
ACTAGTTACC ATGGATCACC GCACGACTTG AGCTATGCCA GTTCGGCGGA CACCTCGCTA 
AATGCCTCGT GGGTTAACAC GAGCGGCCAC TCCCCGCACA CGCCCTACTA TTCGGCGGCC 
CAGATCTATA TGCGACCCAA GGGCGGCAGC ACCACGCCCA CGCCCAGTTG CAGTGGCAGC 
ACAGGTAGCG GCAGCGGAGG CAGCGGGAGT GGCAGTAGCA AGAAGGTGCC ACCAGAGGTG 
CCCAAACGCA CCAGTTCCAT TACGGCACAA CAGCAGACAC AGCTTCTTTT GCTGCAGCGC 
CAGACACCGC CGCCTCCTTC GCTGCTAAGG ACCAATGGCC TGTGCAAAAC CGCCGAGAAC 
GGCAGTCTGA CCTCCGTGCA GAGTTCCGGA TCGGATTCTA GTGTTACCTC TGCGGAACGC 
AATCTAAACA GCGATTTGGG CTCGGATCGC AGTAACTCAC CACATACATG GAAACGAGGA 
ACAGCCCTAA ATAGTTCCCA GCAGTTCTCC ACGCACTCGG CGGATTCAGC GGGTGCGGTG 
TCTGGTGGAG GAGTTGGAGT GGCTGGAGGA GCCGGCGTTT ATGCCGCTCA AATGCAGGCT 
GCCGTTGCAG CAGCTACAGC GGCAGGAGGA ATGCCACCAG CGGATGACCA TGCTATCTCC 
TCGCATACGA GTGCCGCTCA GTATGAGCAG CATGAGCAGC AGCAGCACGA GCAACAGCAA 
TTGCAGGCGG CAGCTGCAGC TGCCGGAGTG GCACAAAACT ACAAGATGTC GGAGACGATA 
CGCAAGCGAC AGTATCGCGT TGGACTCAAT CTGTTCAACA AGAAGCCGGA GAAGGGCATC 
ACCTATCTGA TCAGGCGGGG ATTCCTTGAG AATACACCAC AGGGCGTTGC TCGTTTCCTC 
ATCACCCGTA AGGGTTTGTC CCGGCAAATG ATTGGCGAGT ATCTGGGAAA TTTGCAGAAC 
CAGTTCAACA TGGCCGTGCT CAGTTGCTTT GCCATGGAGC TGGACCTGTC CGGCCGGCAA 
GTGGATGTGG CTTTGCGAAA GTTCCAGGCC TATTTCCGCA TGCCTGGAGA GGCACAAAAG 
ATTGAGCGCC TCATGGAGAT CTTTTCACAG CGCTATTGTG AATGCAATGC GGACATTGTC 
GGGCGATTGA GATCATCCGA TACGATCTTC GTCCTGGCTT TCGCCATCAT CATGCTGAAC 
ACGGATCTGC ACACACCCAA TCTAAAGCCA GAACGTCGCA TGCGCGTCGA GGACTTTATC 
AAGAATCTGC GCGGCATCGA CGACTGTCAC GACATTGATA AGGACATGCT GATGGGCATC 
TATGACCGTG TCAAGTCCGA CGAATTTAAG CCCGGCAGCG ACCATGTCAC TCAAGTGATG 
AAGGTACAGG CCACTATTGT GGGCAAGAAA CCAAATCTAG CGCTGCCCCA TCGGCGTCTT 
GTCTGCTATT GCCGACTGTA CGAGATTCCT GACGTGAACA AGAAGGAGCG ACCTGGTGTG 
CATCAGCGCG AGGTGTTTCT GTTCAACGAT CTGCTGGTCA TTACCAAAAT ATTTAGCAAA 
AAGAAGACCT CCGTGACGTA CACATTCCGC AACAGTTTCC CGCTATGCGG CACCGTTGTC 
ACCCTTCTGG ACATGCCCAA CTATCCGTTT TGCATTCAGC TCTCCCAGAA AGTGGATGGC 
AAGATCTTGA TCACCTTCAA CGCCCGCAAC GAACACGATC GCTGCAAGTT TGCCGAGGAT 
CTTAAAGAGT CCATTAGCGA GATGGACGAG ATGGAGTCGC TGCGCATTGA GGCCGAACTG 
GAGCGCCAGA AGTCGGCGCG CAATCGAGCA CCTGGCAATG CGGAGAATCG TGACAGTGGC 
GTAGCCGATG TGGAGGTCTG CCCATGCCCG TATCAGCAAG GATCACAAGC ATCTGGCGAG 
CAGGCTCCAA ACTCTGCCGA TAACTCGCAG CAGCTGAAGC GCAGTGCGCT GAGCAACAGC 
CTTCTCGACA TGCACGAGCA GTTTGGCAAT GAGAAACCTC AACGTCGTGG CAGCGTTGGC 
TCCCTGGACA GCGGCATGAG CATCTCGTTC CAGTCCACTA CAACATCCAG CGCTTCGAGG 
GAAAATGCCG CTGCCATTGC GGCCGCAGCA AATGCAGCTG CAGCAGCCAA GATGCGATTT 
AACATGCCGC CAACGGCAGC GATTGCCACG CCCAGCAATG TGTATGCAGC TCCGGGAATG 
CAGGCGTATA CCCATGCCAA CTTTGTGCAG CAGTCACAGG CCGCCTACAT GTTGCAGCAA 
CAGCAAATGC TCCAGCAGCA GGCACAAATG CAAGCTCAGG CTCAGGCTCA AGCCCAAGCT 
CAGGCCCAAG CTCAAGCCCA GGCTCAGGCG CAGGCGCAGG CGCAGCCGCT TACTGGCCGA 
ATACCGGGAC GCGAGCGAAA GGCTTCGCGG ACGGATGAGA ACGGACGGTC GACGGAGGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

siz ( NM_168881.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730594.1 Drosophila melanogaster schizo (siz), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa21165
Clone ID Related Accession (Same CDS sequence)	NM_168881.3
Accession Version	NM_168881.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	schizo, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168881.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGAGG CCGATCTGAA GAATCTATCC CAGAACTCGG ATGGCGACCA GGAATTGCTG 
CTGCATCAGA CCACGCAGTC CCTGCTGATG GCCAGCTCCA TAATGTTTGT GGGAAACCAA 
GGAGGGCCGG GCGGTTCCGC TGGGGGTGTC GCCCCACCTG GACCCGTTTC CCTGCCCCTG 
CCCCTCTCCA TCAACATGCC CCATCACCAT GTGCATGCGA TGCCCTACAA TGTGGACGAG 
CTGCTCCGGG AGAACAATGC CCTGCACGCC AAGATCAAGG AGTTGAGTCT GGAACGCGAT 
CGCCTGCTCT GTGAAGTGTC CAATCTGCGA CTGGAGCTGG ACATGTCGGA GCTCAAGCGA 
CTGCCCGTCG ATCTCGATGA TAACTTTCCC CAGAAGAGCC TGGAGCGAAG TGGTTCCACC 
CAGTATGAGC TGGCTGGAGC GCAACAGCCA GGATCAGCCA ACGCCTCCAC CTGCACAGAT 
AGCGGCAGTG TTGGCGGCTA TGTTTACCTG CAGAATCACT ACGCCCCCGG CGCCCACAGC 
TCCTCAGCCG CCATCAACTA CCCTGCGCAA CAGCATCCCC AGATGGTCTA CCAAATACAG 
CAGTATCCCA CGTGCCATCA GCAGCAGCAA CAGCAGCACC TCCAGCAGCA GCAGCATTTG 
CATCAGACCG GCGCAGGTCA CTACATGCAG GTGACGGCGA CGGGTGGTGG CCAGTATCAT 
CACCATCACA TGCTCCATGG TCACGGGCAT CATGCCCACC ATCACGGTGG GGCGGTGGTC 
ATCGCCGGGA GTGGTGTGGG AACTGGCCTG GGATCTGGAG CCACCAGCGT GATCATGCAG 
CACCAGCAAC AGCAGCAACA GCAACAGAAT ATGCACAAGA AGAACTCCAT CCGGAACGGC 
GGAGATGTCC TCAAGCGAAC GCGAGCTCAG TCGGCCTACG AACTCTCACA AGATCTGCTT 
GAGAAACAGA TCGAGCTGCT GGAGCGCAAG TACGGCGGAG TGCGAGCCCG TAACGCAGCG 
GTCACTATTC AGCGCGCCTT CCGTCACTAC ATGATGGTCA AGAAGTTCGC CTCGATCACG 
GCAATGGCCA AAGCTGAGAA GCGTCTAAGC CGGCGGATGG TGGTTACAGC CAGCAGTCTT 
GGATTGGCGG AGGAGGGTGC CTCCTCCTCG TCAGCCTACG GAAGTGCCAC GGAATCTCAG 
CTCACCGAGC AGCAACAGCA ACAACAGGCG CAGCAGCAAC AGCAGCCACG TGTCACCATC 
ATGGCGGGTC CGGCGGGAGC AGCTTCTCCG GGCTTATCCC GGACGCCACC TACGCGATCG 
CTTTCCATGC GGGAGCGACG TCAGCTGGAC TGCAGTCCCA TACCGCGTAG TCAGTCAGGA 
GCCTCTCCCG CCTCCATATC GAGCTCGACA GTCAGCACAT CGGCTCTGGC CTCACATCCA 
CATGTTAATC TGCTGCACGC GGCAGAGCCA CATTATTATA ATGCTCAGGC ACTGCCCCAG 
GGAGCTGCTT ACTACACTAG TTACCATGGA TCACCGCACG ACTTGAGCTA TGCCAGTTCG 
GCGGACACCT CGCTAAATGC CTCGTGGGTT AACACGAGCG GCCACTCCCC GCACACGCCC 
TACTATTCGG CGGCCCAGAT CTATATGCGA CCCAAGGGCG GCAGCACCAC GCCCACGCCC 
AGTTGCAGTG GCAGCACAGG TAGCGGCAGC GGAGGCAGCG GGAGTGGCAG TAGCAAGAAG 
GTGCCACCAG AGGTGCCCAA ACGCACCAGT TCCATTACGG CACAACAGCA GACACAGCTT 
CTTTTGCTGC AGCGCCAGAC ACCGCCGCCT CCTTCGCTGC TAAGGACCAA TGGCCTGTGC 
AAAACCGCCG AGAACGGCAG TCTGACCTCC GTGCAGAGTT CCGGATCGGA TTCTAGTGTT 
ACCTCTGCGG AACGCAATCT AAACAGCGAT TTGGGCTCGG ATCGCAGTAA CTCACCACAT 
ACATGGAAAC GAGGAACAGC CCTAAATAGT TCCCAGCAGT TCTCCACGCA CTCGGCGGAT 
TCAGCGGGTG CGGTGTCTGG TGGAGGAGTT GGAGTGGCTG GAGGAGCCGG CGTTTATGCC 
GCTCAAATGC AGGCTGCCGT TGCAGCAGCT ACAGCGGCAG GAGGAATGCC ACCAGCGGAT 
GACCATGCTA TCTCCTCGCA TACGAGTGCC GCTCAGTATG AGCAGCATGA GCAGCAGCAG 
CACGAGCAAC AGCAATTGCA GGCGGCAGCT GCAGCTGCCG GAGTGGCACA AAACTACAAG 
ATGTCGGAGA CGATACGCAA GCGACAGTAT CGCGTTGGAC TCAATCTGTT CAACAAGAAG 
CCGGAGAAGG GCATCACCTA TCTGATCAGG CGGGGATTCC TTGAGAATAC ACCACAGGGC 
GTTGCTCGTT TCCTCATCAC CCGTAAGGGT TTGTCCCGGC AAATGATTGG CGAGTATCTG 
GGAAATTTGC AGAACCAGTT CAACATGGCC GTGCTCAGTT GCTTTGCCAT GGAGCTGGAC 
CTGTCCGGCC GGCAAGTGGA TGTGGCTTTG CGAAAGTTCC AGGCCTATTT CCGCATGCCT 
GGAGAGGCAC AAAAGATTGA GCGCCTCATG GAGATCTTTT CACAGCGCTA TTGTGAATGC 
AATGCGGACA TTGTCGGGCG ATTGAGATCA TCCGATACGA TCTTCGTCCT GGCTTTCGCC 
ATCATCATGC TGAACACGGA TCTGCACACA CCCAATCTAA AGCCAGAACG TCGCATGCGC 
GTCGAGGACT TTATCAAGAA TCTGCGCGGC ATCGACGACT GTCACGACAT TGATAAGGAC 
ATGCTGATGG GCATCTATGA CCGTGTCAAG TCCGACGAAT TTAAGCCCGG CAGCGACCAT 
GTCACTCAAG TGATGAAGGT ACAGGCCACT ATTGTGGGCA AGAAACCAAA TCTAGCGCTG 
CCCCATCGGC GTCTTGTCTG CTATTGCCGA CTGTACGAGA TTCCTGACGT GAACAAGAAG 
GAGCGACCTG GTGTGCATCA GCGCGAGGTG TTTCTGTTCA ACGATCTGCT GGTCATTACC 
AAAATATTTA GCAAAAAGAA GACCTCCGTG ACGTACACAT TCCGCAACAG TTTCCCGCTA 
TGCGGCACCG TTGTCACCCT TCTGGACATG CCCAACTATC CGTTTTGCAT TCAGCTCTCC 
CAGAAAGTGG ATGGCAAGAT CTTGATCACC TTCAACGCCC GCAACGAACA CGATCGCTGC 
AAGTTTGCCG AGGATCTTAA AGAGTCCATT AGCGAGATGG ACGAGATGGA GTCGCTGCGC 
ATTGAGGCCG AACTGGAGCG CCAGAAGTCG GCGCGCAATC GAGCACCTGG CAATGCGGAG 
AATCGTGACA GTGGCGTAGC CGATGTGGAG GTCTGCCCAT GCCCGTATCA GCAAGGATCA 
CAAGCATCTG GCGAGCAGGC TCCAAACTCT GCCGATAACT CGCAGCAGCT GAAGCGCAGT 
GCGCTGAGCA ACAGCCTTCT CGACATGCAC GAGCAGTTTG GCAATGAGAA ACCTCAACGT 
CGTGGCAGCG TTGGCTCCCT GGACAGCGGC ATGAGCATCT CGTTCCAGTC CACTACAACA 
TCCAGCGCTT CGAGGGAAAA TGCCGCTGCC ATTGCGGCCG CAGCAAATGC AGCTGCAGCA 
GCCAAGATGC GATTTAACAT GCCGCCAACG GCAGCGATTG CCACGCCCAG CAATGTGTAT 
GCAGCTCCGG GAATGCAGGC GTATACCCAT GCCAACTTTG TGCAGCAGTC ACAGGCCGCC 
TACATGTTGC AGCAACAGCA AATGCTCCAG CAGCAGGCAC AAATGCAAGC TCAGGCTCAG 
GCTCAAGCCC AAGCTCAGGC CCAAGCTCAA GCCCAGGCTC AGGCGCAGGC GCAGGCGCAG 
CCGCTTACTG GCCGAATACC GGGACGCGAG CGAAAGGCTT CGCGGACGGA TGAGAACGGA 
CGGTCGACGG AGGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730594.1
CDS	385..4362
Translation	MSEADLKNLSQNSDGDQELLLHQTTQSLLMASSIMFVGNQGGPGGSAGGVAPPGPVSLPLPLSINMPHHHVHAMPYNVDELLRENNALHAKIKELSLERDRLLCEVSNLRLELDMSELKRLPVDLDDNFPQKSLERSGSTQYELAGAQQPGSANASTCTDSGSVGGYVYLQNHYAPGAHSSSAAINYPAQQHPQMVYQIQQYPTCHQQQQQQHLQQQQHLHQTGAGHYMQVTATGGGQYHHHHMLHGHGHHAHHHGGAVVIAGSGVGTGLGSGATSVIMQHQQQQQQQQNMHKKNSIRNGGDVLKRTRAQSAYELSQDLLEKQIELLERKYGGVRARNAAVTIQRAFRHYMMVKKFASITAMAKAEKRLSRRMVVTASSLGLAEEGASSSSAYGSATESQLTEQQQQQQAQQQQQPRVTIMAGPAGAASPGLSRTPPTRSLSMRERRQLDCSPIPRSQSGASPASISSSTVSTSALASHPHVNLLHAAEPHYYNAQALPQGAAYYTSYHGSPHDLSYASSADTSLNASWVNTSGHSPHTPYYSAAQIYMRPKGGSTTPTPSCSGSTGSGSGGSGSGSSKKVPPEVPKRTSSITAQQQTQLLLLQRQTPPPPSLLRTNGLCKTAENGSLTSVQSSGSDSSVTSAERNLNSDLGSDRSNSPHTWKRGTALNSSQQFSTHSADSAGAVSGGGVGVAGGAGVYAAQMQAAVAAATAAGGMPPADDHAISSHTSAAQYEQHEQQQHEQQQLQAAAAAAGVAQNYKMSETIRKRQYRVGLNLFNKKPEKGITYLIRRGFLENTPQGVARFLITRKGLSRQMIGEYLGNLQNQFNMAVLSCFAMELDLSGRQVDVALRKFQAYFRMPGEAQKIERLMEIFSQRYCECNADIVGRLRSSDTIFVLAFAIIMLNTDLHTPNLKPERRMRVEDFIKNLRGIDDCHDIDKDMLMGIYDRVKSDEFKPGSDHVTQVMKVQATIVGKKPNLALPHRRLVCYCRLYEIPDVNKKERPGVHQREVFLFNDLLVITKIFSKKKTSVTYTFRNSFPLCGTVVTLLDMPNYPFCIQLSQKVDGKILITFNARNEHDRCKFAEDLKESISEMDEMESLRIEAELERQKSARNRAPGNAENRDSGVADVEVCPCPYQQGSQASGEQAPNSADNSQQLKRSALSNSLLDMHEQFGNEKPQRRGSVGSLDSGMSISFQSTTTSSASRENAAAIAAAANAAAAAKMRFNMPPTAAIATPSNVYAAPGMQAYTHANFVQQSQAAYMLQQQQMLQQQAQMQAQAQAQAQAQAQAQAQAQAQAQAQPLTGRIPGRERKASRTDENGRSTEV

Target ORF information:

RefSeq Version	NM_168881.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster schizo (siz), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168881.3

ORF Insert Sequence:

ATGTCCGAGG CCGATCTGAA GAATCTATCC CAGAACTCGG ATGGCGACCA GGAATTGCTG 
CTGCATCAGA CCACGCAGTC CCTGCTGATG GCCAGCTCCA TAATGTTTGT GGGAAACCAA 
GGAGGGCCGG GCGGTTCCGC TGGGGGTGTC GCCCCACCTG GACCCGTTTC CCTGCCCCTG 
CCCCTCTCCA TCAACATGCC CCATCACCAT GTGCATGCGA TGCCCTACAA TGTGGACGAG 
CTGCTCCGGG AGAACAATGC CCTGCACGCC AAGATCAAGG AGTTGAGTCT GGAACGCGAT 
CGCCTGCTCT GTGAAGTGTC CAATCTGCGA CTGGAGCTGG ACATGTCGGA GCTCAAGCGA 
CTGCCCGTCG ATCTCGATGA TAACTTTCCC CAGAAGAGCC TGGAGCGAAG TGGTTCCACC 
CAGTATGAGC TGGCTGGAGC GCAACAGCCA GGATCAGCCA ACGCCTCCAC CTGCACAGAT 
AGCGGCAGTG TTGGCGGCTA TGTTTACCTG CAGAATCACT ACGCCCCCGG CGCCCACAGC 
TCCTCAGCCG CCATCAACTA CCCTGCGCAA CAGCATCCCC AGATGGTCTA CCAAATACAG 
CAGTATCCCA CGTGCCATCA GCAGCAGCAA CAGCAGCACC TCCAGCAGCA GCAGCATTTG 
CATCAGACCG GCGCAGGTCA CTACATGCAG GTGACGGCGA CGGGTGGTGG CCAGTATCAT 
CACCATCACA TGCTCCATGG TCACGGGCAT CATGCCCACC ATCACGGTGG GGCGGTGGTC 
ATCGCCGGGA GTGGTGTGGG AACTGGCCTG GGATCTGGAG CCACCAGCGT GATCATGCAG 
CACCAGCAAC AGCAGCAACA GCAACAGAAT ATGCACAAGA AGAACTCCAT CCGGAACGGC 
GGAGATGTCC TCAAGCGAAC GCGAGCTCAG TCGGCCTACG AACTCTCACA AGATCTGCTT 
GAGAAACAGA TCGAGCTGCT GGAGCGCAAG TACGGCGGAG TGCGAGCCCG TAACGCAGCG 
GTCACTATTC AGCGCGCCTT CCGTCACTAC ATGATGGTCA AGAAGTTCGC CTCGATCACG 
GCAATGGCCA AAGCTGAGAA GCGTCTAAGC CGGCGGATGG TGGTTACAGC CAGCAGTCTT 
GGATTGGCGG AGGAGGGTGC CTCCTCCTCG TCAGCCTACG GAAGTGCCAC GGAATCTCAG 
CTCACCGAGC AGCAACAGCA ACAACAGGCG CAGCAGCAAC AGCAGCCACG TGTCACCATC 
ATGGCGGGTC CGGCGGGAGC AGCTTCTCCG GGCTTATCCC GGACGCCACC TACGCGATCG 
CTTTCCATGC GGGAGCGACG TCAGCTGGAC TGCAGTCCCA TACCGCGTAG TCAGTCAGGA 
GCCTCTCCCG CCTCCATATC GAGCTCGACA GTCAGCACAT CGGCTCTGGC CTCACATCCA 
CATGTTAATC TGCTGCACGC GGCAGAGCCA CATTATTATA ATGCTCAGGC ACTGCCCCAG 
GGAGCTGCTT ACTACACTAG TTACCATGGA TCACCGCACG ACTTGAGCTA TGCCAGTTCG 
GCGGACACCT CGCTAAATGC CTCGTGGGTT AACACGAGCG GCCACTCCCC GCACACGCCC 
TACTATTCGG CGGCCCAGAT CTATATGCGA CCCAAGGGCG GCAGCACCAC GCCCACGCCC 
AGTTGCAGTG GCAGCACAGG TAGCGGCAGC GGAGGCAGCG GGAGTGGCAG TAGCAAGAAG 
GTGCCACCAG AGGTGCCCAA ACGCACCAGT TCCATTACGG CACAACAGCA GACACAGCTT 
CTTTTGCTGC AGCGCCAGAC ACCGCCGCCT CCTTCGCTGC TAAGGACCAA TGGCCTGTGC 
AAAACCGCCG AGAACGGCAG TCTGACCTCC GTGCAGAGTT CCGGATCGGA TTCTAGTGTT 
ACCTCTGCGG AACGCAATCT AAACAGCGAT TTGGGCTCGG ATCGCAGTAA CTCACCACAT 
ACATGGAAAC GAGGAACAGC CCTAAATAGT TCCCAGCAGT TCTCCACGCA CTCGGCGGAT 
TCAGCGGGTG CGGTGTCTGG TGGAGGAGTT GGAGTGGCTG GAGGAGCCGG CGTTTATGCC 
GCTCAAATGC AGGCTGCCGT TGCAGCAGCT ACAGCGGCAG GAGGAATGCC ACCAGCGGAT 
GACCATGCTA TCTCCTCGCA TACGAGTGCC GCTCAGTATG AGCAGCATGA GCAGCAGCAG 
CACGAGCAAC AGCAATTGCA GGCGGCAGCT GCAGCTGCCG GAGTGGCACA AAACTACAAG 
ATGTCGGAGA CGATACGCAA GCGACAGTAT CGCGTTGGAC TCAATCTGTT CAACAAGAAG 
CCGGAGAAGG GCATCACCTA TCTGATCAGG CGGGGATTCC TTGAGAATAC ACCACAGGGC 
GTTGCTCGTT TCCTCATCAC CCGTAAGGGT TTGTCCCGGC AAATGATTGG CGAGTATCTG 
GGAAATTTGC AGAACCAGTT CAACATGGCC GTGCTCAGTT GCTTTGCCAT GGAGCTGGAC 
CTGTCCGGCC GGCAAGTGGA TGTGGCTTTG CGAAAGTTCC AGGCCTATTT CCGCATGCCT 
GGAGAGGCAC AAAAGATTGA GCGCCTCATG GAGATCTTTT CACAGCGCTA TTGTGAATGC 
AATGCGGACA TTGTCGGGCG ATTGAGATCA TCCGATACGA TCTTCGTCCT GGCTTTCGCC 
ATCATCATGC TGAACACGGA TCTGCACACA CCCAATCTAA AGCCAGAACG TCGCATGCGC 
GTCGAGGACT TTATCAAGAA TCTGCGCGGC ATCGACGACT GTCACGACAT TGATAAGGAC 
ATGCTGATGG GCATCTATGA CCGTGTCAAG TCCGACGAAT TTAAGCCCGG CAGCGACCAT 
GTCACTCAAG TGATGAAGGT ACAGGCCACT ATTGTGGGCA AGAAACCAAA TCTAGCGCTG 
CCCCATCGGC GTCTTGTCTG CTATTGCCGA CTGTACGAGA TTCCTGACGT GAACAAGAAG 
GAGCGACCTG GTGTGCATCA GCGCGAGGTG TTTCTGTTCA ACGATCTGCT GGTCATTACC 
AAAATATTTA GCAAAAAGAA GACCTCCGTG ACGTACACAT TCCGCAACAG TTTCCCGCTA 
TGCGGCACCG TTGTCACCCT TCTGGACATG CCCAACTATC CGTTTTGCAT TCAGCTCTCC 
CAGAAAGTGG ATGGCAAGAT CTTGATCACC TTCAACGCCC GCAACGAACA CGATCGCTGC 
AAGTTTGCCG AGGATCTTAA AGAGTCCATT AGCGAGATGG ACGAGATGGA GTCGCTGCGC 
ATTGAGGCCG AACTGGAGCG CCAGAAGTCG GCGCGCAATC GAGCACCTGG CAATGCGGAG 
AATCGTGACA GTGGCGTAGC CGATGTGGAG GTCTGCCCAT GCCCGTATCA GCAAGGATCA 
CAAGCATCTG GCGAGCAGGC TCCAAACTCT GCCGATAACT CGCAGCAGCT GAAGCGCAGT 
GCGCTGAGCA ACAGCCTTCT CGACATGCAC GAGCAGTTTG GCAATGAGAA ACCTCAACGT 
CGTGGCAGCG TTGGCTCCCT GGACAGCGGC ATGAGCATCT CGTTCCAGTC CACTACAACA 
TCCAGCGCTT CGAGGGAAAA TGCCGCTGCC ATTGCGGCCG CAGCAAATGC AGCTGCAGCA 
GCCAAGATGC GATTTAACAT GCCGCCAACG GCAGCGATTG CCACGCCCAG CAATGTGTAT 
GCAGCTCCGG GAATGCAGGC GTATACCCAT GCCAACTTTG TGCAGCAGTC ACAGGCCGCC 
TACATGTTGC AGCAACAGCA AATGCTCCAG CAGCAGGCAC AAATGCAAGC TCAGGCTCAG 
GCTCAAGCCC AAGCTCAGGC CCAAGCTCAA GCCCAGGCTC AGGCGCAGGC GCAGGCGCAG 
CCGCTTACTG GCCGAATACC GGGACGCGAG CGAAAGGCTT CGCGGACGGA TGAGAACGGA 
CGGTCGACGG AGGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

siz ( NM_206407.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996129.1 Drosophila melanogaster schizo (siz), transcript variant A, mRNA.

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CloneID	OFa21167
Clone ID Related Accession (Same CDS sequence)	NM_206407.2
Accession Version	NM_206407.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	schizo, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Dec 17, 2009 this sequence version replaced NM_206407.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAGGT GTGACAACCA CAAATCCCGC CGCCAACAGC TGCCCGTTCC GGCGCCCCTG 
TCCTCCTCCC ACTCCAACTC GTCCTCGTCC CGCTCGCAAG GACACCACCA CCACCACCAC 
CACCAGCTCC ATTCGAGCAA CTCGGATCTG GGCGGCAGTG AGTCCTTCCT GCAATATTGC 
AGCGATAGCG AAAAGAGGCC ACCACCGATT GTGGTGGTTG TGGGCGATGG CAGGAGCCGG 
GTACGTCGGG TGGTGCGCAC CGCAACGCGG CACGTCACCG TGGTCAGTCT GTCCACCCGG 
CACAAGGAGA CCCAGACGCA CACCTCGCAC CATGTGACGG CCGTCAGCTT TCCCCAGAAG 
AGCCTGGAGC GAAGTGGTTC CACCCAGTAT GAGCTGGCTG GAGCGCAACA GCCAGGATCA 
GCCAACGCCT CCACCTGCAC AGATAGCGGC AGTGTTGGCG GCTATGTTTA CCTGCAGAAT 
CACTACGCCC CCGGCGCCCA CAGCTCCTCA GCCGCCATCA ACTACCCTGC GCAACAGCAT 
CCCCAGATGG TCTACCAAAT ACAGCAGTAT CCCACGTGCC ATCAGCAGCA GCAACAGCAG 
CACCTCCAGC AGCAGCAGCA TTTGCATCAG ACCGGCGCAG GTCACTACAT GCAGGTGACG 
GCGACGGGTG GTGGCCAGTA TCATCACCAT CACATGCTCC ATGGTCACGG GCATCATGCC 
CACCATCACG GTGGGGCGGT GGTCATCGCC GGGAGTGGTG TGGGAACTGG CCTGGGATCT 
GGAGCCACCA GCGTGATCAT GCAGCACCAG CAACAGCAGC AACAGCAACA GAATATGCAC 
AAGAAGAACT CCATCCGGAA CGGCGGAGAT GTCCTCAAGC GAACGCGAGC TCAGTCGGCC 
TACGAACTCT CACAAGATCT GCTTGAGAAA CAGATCGAGC TGCTGGAGCG CAAGTACGGC 
GGAGTGCGAG CCCGTAACGC AGCGGTCACT ATTCAGCGCG CCTTCCGTCA CTACATGATG 
GTCAAGAAGT TCGCCTCGAT CACGGCAATG GCCAAAGCTG AGAAGCGTCT AAGCCGGCGG 
ATGGTGGTTA CAGCCAGCAG TCTTGGATTG GCGGAGGAGG GTGCCTCCTC CTCGTCAGCC 
TACGGAAGTG CCACGGAATC TCAGCTCACC GAGCAGCAAC AGCAACAACA GGCGCAGCAG 
CAACAGCAGC CACGTGTCAC CATCATGGCG GGTCCGGCGG GAGCAGCTTC TCCGGGCTTA 
TCCCGGACGC CACCTACGCG ATCGCTTTCC ATGCGGGAGC GACGTCAGCT GGACTGCAGT 
CCCATACCGC GTAGTCAGTC AGGAGCCTCT CCCGCCTCCA TATCGAGCTC GACAGTCAGC 
ACATCGGCTC TGGCCTCACA TCCACATGTT AATCTGCTGC ACGCGGCAGA GCCACATTAT 
TATAATGCTC AGGCACTGCC CCAGGGAGCT GCTTACTACA CTAGTTACCA TGGATCACCG 
CACGACTTGA GCTATGCCAG TTCGGCGGAC ACCTCGCTAA ATGCCTCGTG GGTTAACACG 
AGCGGCCACT CCCCGCACAC GCCCTACTAT TCGGCGGCCC AGATCTATAT GCGACCCAAG 
GGCGGCAGCA CCACGCCCAC GCCCAGTTGC AGTGGCAGCA CAGGTAGCGG CAGCGGAGGC 
AGCGGGAGTG GCAGTAGCAA GAAGGTGCCA CCAGAGGTGC CCAAACGCAC CAGTTCCATT 
ACGGCACAAC AGCAGACACA GCTTCTTTTG CTGCAGCGCC AGACACCGCC GCCTCCTTCG 
CTGCTAAGGA CCAATGGCCT GTGCAAAACC GCCGAGAACG GCAGTCTGAC CTCCGTGCAG 
AGTTCCGGAT CGGATTCTAG TGTTACCTCT GCGGAACGCA ATCTAAACAG CGATTTGGGC 
TCGGATCGCA GTAACTCACC ACATACATGG AAACGAGGAA CAGCCCTAAA TAGTTCCCAG 
CAGTTCTCCA CGCACTCGGC GGATTCAGCG GGTGCGGTGT CTGGTGGAGG AGTTGGAGTG 
GCTGGAGGAG CCGGCGTTTA TGCCGCTCAA ATGCAGGCTG CCGTTGCAGC AGCTACAGCG 
GCAGGAGGAA TGCCACCAGC GGATGACCAT GCTATCTCCT CGCATACGAG TGCCGCTCAG 
TATGAGCAGC ATGAGCAGCA GCAGCACGAG CAACAGCAAT TGCAGGCGGC AGCTGCAGCT 
GCCGGAGTGG CACAAAACTA CAAGATGTCG GAGACGATAC GCAAGCGACA GTATCGCGTT 
GGACTCAATC TGTTCAACAA GAAGCCGGAG AAGGGCATCA CCTATCTGAT CAGGCGGGGA 
TTCCTTGAGA ATACACCACA GGGCGTTGCT CGTTTCCTCA TCACCCGTAA GGGTTTGTCC 
CGGCAAATGA TTGGCGAGTA TCTGGGAAAT TTGCAGAACC AGTTCAACAT GGCCGTGCTC 
AGTTGCTTTG CCATGGAGCT GGACCTGTCC GGCCGGCAAG TGGATGTGGC TTTGCGAAAG 
TTCCAGGCCT ATTTCCGCAT GCCTGGAGAG GCACAAAAGA TTGAGCGCCT CATGGAGATC 
TTTTCACAGC GCTATTGTGA ATGCAATGCG GACATTGTCG GGCGATTGAG ATCATCCGAT 
ACGATCTTCG TCCTGGCTTT CGCCATCATC ATGCTGAACA CGGATCTGCA CACACCCAAT 
CTAAAGCCAG AACGTCGCAT GCGCGTCGAG GACTTTATCA AGAATCTGCG CGGCATCGAC 
GACTGTCACG ACATTGATAA GGACATGCTG ATGGGCATCT ATGACCGTGT CAAGTCCGAC 
GAATTTAAGC CCGGCAGCGA CCATGTCACT CAAGTGATGA AGGTACAGGC CACTATTGTG 
GGCAAGAAAC CAAATCTAGC GCTGCCCCAT CGGCGTCTTG TCTGCTATTG CCGACTGTAC 
GAGATTCCTG ACGTGAACAA GAAGGAGCGA CCTGGTGTGC ATCAGCGCGA GGTGTTTCTG 
TTCAACGATC TGCTGGTCAT TACCAAAATA TTTAGCAAAA AGAAGACCTC CGTGACGTAC 
ACATTCCGCA ACAGTTTCCC GCTATGCGGC ACCGTTGTCA CCCTTCTGGA CATGCCCAAC 
TATCCGTTTT GCATTCAGCT CTCCCAGAAA GTGGATGGCA AGATCTTGAT CACCTTCAAC 
GCCCGCAACG AACACGATCG CTGCAAGTTT GCCGAGGATC TTAAAGAGTC CATTAGCGAG 
ATGGACGAGA TGGAGTCGCT GCGCATTGAG GCCGAACTGG AGCGCCAGAA GTCGGCGCGC 
AATCGAGCAC CTGGCAATGC GGAGAATCGT GACAGTGGCG TAGCCGATGT GGAGGTCTGC 
CCATGCCCGT ATCAGCAAGG ATCACAAGCA TCTGGCGAGC AGGCTCCAAA CTCTGCCGAT 
AACTCGCAGC AGCTGAAGCG CAGTGCGCTG AGCAACAGCC TTCTCGACAT GCACGAGCAG 
TTTGGCAATG AGAAACCTCA ACGTCGTGGC AGCGTTGGCT CCCTGGACAG CGGCATGAGC 
ATCTCGTTCC AGTCCACTAC AACATCCAGC GCTTCGAGGG AAAATGCCGC TGCCATTGCG 
GCCGCAGCAA ATGCAGCTGC AGCAGCCAAG ATGCGATTTA ACATGCCGCC AACGGCAGCG 
ATTGCCACGC CCAGCAATGT GTATGCAGCT CCGGGAATGC AGGCGTATAC CCATGCCAAC 
TTTGTGCAGC AGTCACAGGC CGCCTACATG TTGCAGCAAC AGCAAATGCT CCAGCAGCAG 
GCACAAATGC AAGCTCAGGC TCAGGCTCAA GCCCAAGCTC AGGCCCAAGC TCAAGCCCAG 
GCTCAGGCGC AGGCGCAGGC GCAGCCGCTT ACTGGCCGAA TACCGGGACG CGAGCGAAAG 
GCTTCGCGGA CGGATGAGAA CGGACGGTCG ACGGAGGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996129.1
CDS	605..4546
Translation	MSRCDNHKSRRQQLPVPAPLSSSHSNSSSSRSQGHHHHHHHQLHSSNSDLGGSESFLQYCSDSEKRPPPIVVVVGDGRSRVRRVVRTATRHVTVVSLSTRHKETQTHTSHHVTAVSFPQKSLERSGSTQYELAGAQQPGSANASTCTDSGSVGGYVYLQNHYAPGAHSSSAAINYPAQQHPQMVYQIQQYPTCHQQQQQQHLQQQQHLHQTGAGHYMQVTATGGGQYHHHHMLHGHGHHAHHHGGAVVIAGSGVGTGLGSGATSVIMQHQQQQQQQQNMHKKNSIRNGGDVLKRTRAQSAYELSQDLLEKQIELLERKYGGVRARNAAVTIQRAFRHYMMVKKFASITAMAKAEKRLSRRMVVTASSLGLAEEGASSSSAYGSATESQLTEQQQQQQAQQQQQPRVTIMAGPAGAASPGLSRTPPTRSLSMRERRQLDCSPIPRSQSGASPASISSSTVSTSALASHPHVNLLHAAEPHYYNAQALPQGAAYYTSYHGSPHDLSYASSADTSLNASWVNTSGHSPHTPYYSAAQIYMRPKGGSTTPTPSCSGSTGSGSGGSGSGSSKKVPPEVPKRTSSITAQQQTQLLLLQRQTPPPPSLLRTNGLCKTAENGSLTSVQSSGSDSSVTSAERNLNSDLGSDRSNSPHTWKRGTALNSSQQFSTHSADSAGAVSGGGVGVAGGAGVYAAQMQAAVAAATAAGGMPPADDHAISSHTSAAQYEQHEQQQHEQQQLQAAAAAAGVAQNYKMSETIRKRQYRVGLNLFNKKPEKGITYLIRRGFLENTPQGVARFLITRKGLSRQMIGEYLGNLQNQFNMAVLSCFAMELDLSGRQVDVALRKFQAYFRMPGEAQKIERLMEIFSQRYCECNADIVGRLRSSDTIFVLAFAIIMLNTDLHTPNLKPERRMRVEDFIKNLRGIDDCHDIDKDMLMGIYDRVKSDEFKPGSDHVTQVMKVQATIVGKKPNLALPHRRLVCYCRLYEIPDVNKKERPGVHQREVFLFNDLLVITKIFSKKKTSVTYTFRNSFPLCGTVVTLLDMPNYPFCIQLSQKVDGKILITFNARNEHDRCKFAEDLKESISEMDEMESLRIEAELERQKSARNRAPGNAENRDSGVADVEVCPCPYQQGSQASGEQAPNSADNSQQLKRSALSNSLLDMHEQFGNEKPQRRGSVGSLDSGMSISFQSTTTSSASRENAAAIAAAANAAAAAKMRFNMPPTAAIATPSNVYAAPGMQAYTHANFVQQSQAAYMLQQQQMLQQQAQMQAQAQAQAQAQAQAQAQAQAQAQAQPLTGRIPGRERKASRTDENGRSTEV

Target ORF information:

RefSeq Version	NM_206407.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster schizo (siz), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206407.2

ORF Insert Sequence:

ATGTCCAGGT GTGACAACCA CAAATCCCGC CGCCAACAGC TGCCCGTTCC GGCGCCCCTG 
TCCTCCTCCC ACTCCAACTC GTCCTCGTCC CGCTCGCAAG GACACCACCA CCACCACCAC 
CACCAGCTCC ATTCGAGCAA CTCGGATCTG GGCGGCAGTG AGTCCTTCCT GCAATATTGC 
AGCGATAGCG AAAAGAGGCC ACCACCGATT GTGGTGGTTG TGGGCGATGG CAGGAGCCGG 
GTACGTCGGG TGGTGCGCAC CGCAACGCGG CACGTCACCG TGGTCAGTCT GTCCACCCGG 
CACAAGGAGA CCCAGACGCA CACCTCGCAC CATGTGACGG CCGTCAGCTT TCCCCAGAAG 
AGCCTGGAGC GAAGTGGTTC CACCCAGTAT GAGCTGGCTG GAGCGCAACA GCCAGGATCA 
GCCAACGCCT CCACCTGCAC AGATAGCGGC AGTGTTGGCG GCTATGTTTA CCTGCAGAAT 
CACTACGCCC CCGGCGCCCA CAGCTCCTCA GCCGCCATCA ACTACCCTGC GCAACAGCAT 
CCCCAGATGG TCTACCAAAT ACAGCAGTAT CCCACGTGCC ATCAGCAGCA GCAACAGCAG 
CACCTCCAGC AGCAGCAGCA TTTGCATCAG ACCGGCGCAG GTCACTACAT GCAGGTGACG 
GCGACGGGTG GTGGCCAGTA TCATCACCAT CACATGCTCC ATGGTCACGG GCATCATGCC 
CACCATCACG GTGGGGCGGT GGTCATCGCC GGGAGTGGTG TGGGAACTGG CCTGGGATCT 
GGAGCCACCA GCGTGATCAT GCAGCACCAG CAACAGCAGC AACAGCAACA GAATATGCAC 
AAGAAGAACT CCATCCGGAA CGGCGGAGAT GTCCTCAAGC GAACGCGAGC TCAGTCGGCC 
TACGAACTCT CACAAGATCT GCTTGAGAAA CAGATCGAGC TGCTGGAGCG CAAGTACGGC 
GGAGTGCGAG CCCGTAACGC AGCGGTCACT ATTCAGCGCG CCTTCCGTCA CTACATGATG 
GTCAAGAAGT TCGCCTCGAT CACGGCAATG GCCAAAGCTG AGAAGCGTCT AAGCCGGCGG 
ATGGTGGTTA CAGCCAGCAG TCTTGGATTG GCGGAGGAGG GTGCCTCCTC CTCGTCAGCC 
TACGGAAGTG CCACGGAATC TCAGCTCACC GAGCAGCAAC AGCAACAACA GGCGCAGCAG 
CAACAGCAGC CACGTGTCAC CATCATGGCG GGTCCGGCGG GAGCAGCTTC TCCGGGCTTA 
TCCCGGACGC CACCTACGCG ATCGCTTTCC ATGCGGGAGC GACGTCAGCT GGACTGCAGT 
CCCATACCGC GTAGTCAGTC AGGAGCCTCT CCCGCCTCCA TATCGAGCTC GACAGTCAGC 
ACATCGGCTC TGGCCTCACA TCCACATGTT AATCTGCTGC ACGCGGCAGA GCCACATTAT 
TATAATGCTC AGGCACTGCC CCAGGGAGCT GCTTACTACA CTAGTTACCA TGGATCACCG 
CACGACTTGA GCTATGCCAG TTCGGCGGAC ACCTCGCTAA ATGCCTCGTG GGTTAACACG 
AGCGGCCACT CCCCGCACAC GCCCTACTAT TCGGCGGCCC AGATCTATAT GCGACCCAAG 
GGCGGCAGCA CCACGCCCAC GCCCAGTTGC AGTGGCAGCA CAGGTAGCGG CAGCGGAGGC 
AGCGGGAGTG GCAGTAGCAA GAAGGTGCCA CCAGAGGTGC CCAAACGCAC CAGTTCCATT 
ACGGCACAAC AGCAGACACA GCTTCTTTTG CTGCAGCGCC AGACACCGCC GCCTCCTTCG 
CTGCTAAGGA CCAATGGCCT GTGCAAAACC GCCGAGAACG GCAGTCTGAC CTCCGTGCAG 
AGTTCCGGAT CGGATTCTAG TGTTACCTCT GCGGAACGCA ATCTAAACAG CGATTTGGGC 
TCGGATCGCA GTAACTCACC ACATACATGG AAACGAGGAA CAGCCCTAAA TAGTTCCCAG 
CAGTTCTCCA CGCACTCGGC GGATTCAGCG GGTGCGGTGT CTGGTGGAGG AGTTGGAGTG 
GCTGGAGGAG CCGGCGTTTA TGCCGCTCAA ATGCAGGCTG CCGTTGCAGC AGCTACAGCG 
GCAGGAGGAA TGCCACCAGC GGATGACCAT GCTATCTCCT CGCATACGAG TGCCGCTCAG 
TATGAGCAGC ATGAGCAGCA GCAGCACGAG CAACAGCAAT TGCAGGCGGC AGCTGCAGCT 
GCCGGAGTGG CACAAAACTA CAAGATGTCG GAGACGATAC GCAAGCGACA GTATCGCGTT 
GGACTCAATC TGTTCAACAA GAAGCCGGAG AAGGGCATCA CCTATCTGAT CAGGCGGGGA 
TTCCTTGAGA ATACACCACA GGGCGTTGCT CGTTTCCTCA TCACCCGTAA GGGTTTGTCC 
CGGCAAATGA TTGGCGAGTA TCTGGGAAAT TTGCAGAACC AGTTCAACAT GGCCGTGCTC 
AGTTGCTTTG CCATGGAGCT GGACCTGTCC GGCCGGCAAG TGGATGTGGC TTTGCGAAAG 
TTCCAGGCCT ATTTCCGCAT GCCTGGAGAG GCACAAAAGA TTGAGCGCCT CATGGAGATC 
TTTTCACAGC GCTATTGTGA ATGCAATGCG GACATTGTCG GGCGATTGAG ATCATCCGAT 
ACGATCTTCG TCCTGGCTTT CGCCATCATC ATGCTGAACA CGGATCTGCA CACACCCAAT 
CTAAAGCCAG AACGTCGCAT GCGCGTCGAG GACTTTATCA AGAATCTGCG CGGCATCGAC 
GACTGTCACG ACATTGATAA GGACATGCTG ATGGGCATCT ATGACCGTGT CAAGTCCGAC 
GAATTTAAGC CCGGCAGCGA CCATGTCACT CAAGTGATGA AGGTACAGGC CACTATTGTG 
GGCAAGAAAC CAAATCTAGC GCTGCCCCAT CGGCGTCTTG TCTGCTATTG CCGACTGTAC 
GAGATTCCTG ACGTGAACAA GAAGGAGCGA CCTGGTGTGC ATCAGCGCGA GGTGTTTCTG 
TTCAACGATC TGCTGGTCAT TACCAAAATA TTTAGCAAAA AGAAGACCTC CGTGACGTAC 
ACATTCCGCA ACAGTTTCCC GCTATGCGGC ACCGTTGTCA CCCTTCTGGA CATGCCCAAC 
TATCCGTTTT GCATTCAGCT CTCCCAGAAA GTGGATGGCA AGATCTTGAT CACCTTCAAC 
GCCCGCAACG AACACGATCG CTGCAAGTTT GCCGAGGATC TTAAAGAGTC CATTAGCGAG 
ATGGACGAGA TGGAGTCGCT GCGCATTGAG GCCGAACTGG AGCGCCAGAA GTCGGCGCGC 
AATCGAGCAC CTGGCAATGC GGAGAATCGT GACAGTGGCG TAGCCGATGT GGAGGTCTGC 
CCATGCCCGT ATCAGCAAGG ATCACAAGCA TCTGGCGAGC AGGCTCCAAA CTCTGCCGAT 
AACTCGCAGC AGCTGAAGCG CAGTGCGCTG AGCAACAGCC TTCTCGACAT GCACGAGCAG 
TTTGGCAATG AGAAACCTCA ACGTCGTGGC AGCGTTGGCT CCCTGGACAG CGGCATGAGC 
ATCTCGTTCC AGTCCACTAC AACATCCAGC GCTTCGAGGG AAAATGCCGC TGCCATTGCG 
GCCGCAGCAA ATGCAGCTGC AGCAGCCAAG ATGCGATTTA ACATGCCGCC AACGGCAGCG 
ATTGCCACGC CCAGCAATGT GTATGCAGCT CCGGGAATGC AGGCGTATAC CCATGCCAAC 
TTTGTGCAGC AGTCACAGGC CGCCTACATG TTGCAGCAAC AGCAAATGCT CCAGCAGCAG 
GCACAAATGC AAGCTCAGGC TCAGGCTCAA GCCCAAGCTC AGGCCCAAGC TCAAGCCCAG 
GCTCAGGCGC AGGCGCAGGC GCAGCCGCTT ACTGGCCGAA TACCGGGACG CGAGCGAAAG 
GCTTCGCGGA CGGATGAGAA CGGACGGTCG ACGGAGGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

siz ( NM_001043153.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036618.1 Drosophila melanogaster schizo (siz), transcript variant C, mRNA.

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CloneID	OFa21168
Clone ID Related Accession (Same CDS sequence)	NM_001043153.2
Accession Version	NM_001043153.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	schizo, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Dec 17, 2009 this sequence version replaced NM_001043153.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATCATTT GCATATCGGG TTTTATAATT GGCGAAAGCT TTCCCCAGAA GAGCCTGGAG 
CGAAGTGGTT CCACCCAGTA TGAGCTGGCT GGAGCGCAAC AGCCAGGATC AGCCAACGCC 
TCCACCTGCA CAGATAGCGG CAGTGTTGGC GGCTATGTTT ACCTGCAGAA TCACTACGCC 
CCCGGCGCCC ACAGCTCCTC AGCCGCCATC AACTACCCTG CGCAACAGCA TCCCCAGATG 
GTCTACCAAA TACAGCAGTA TCCCACGTGC CATCAGCAGC AGCAACAGCA GCACCTCCAG 
CAGCAGCAGC ATTTGCATCA GACCGGCGCA GGTCACTACA TGCAGGTGAC GGCGACGGGT 
GGTGGCCAGT ATCATCACCA TCACATGCTC CATGGTCACG GGCATCATGC CCACCATCAC 
GGTGGGGCGG TGGTCATCGC CGGGAGTGGT GTGGGAACTG GCCTGGGATC TGGAGCCACC 
AGCGTGATCA TGCAGCACCA GCAACAGCAG CAACAGCAAC AGAATATGCA CAAGAAGAAC 
TCCATCCGGA ACGGCGGAGA TGTCCTCAAG CGAACGCGAG CTCAGTCGGC CTACGAACTC 
TCACAAGATC TGCTTGAGAA ACAGATCGAG CTGCTGGAGC GCAAGTACGG CGGAGTGCGA 
GCCCGTAACG CAGCGGTCAC TATTCAGCGC GCCTTCCGTC ACTACATGAT GGTCAAGAAG 
TTCGCCTCGA TCACGGCAAT GGCCAAAGCT GAGAAGCGTC TAAGCCGGCG GATGGTGGTT 
ACAGCCAGCA GTCTTGGATT GGCGGAGGAG GGTGCCTCCT CCTCGTCAGC CTACGGAAGT 
GCCACGGAAT CTCAGCTCAC CGAGCAGCAA CAGCAACAAC AGGCGCAGCA GCAACAGCAG 
CCACGTGTCA CCATCATGGC GGGTCCGGCG GGAGCAGCTT CTCCGGGCTT ATCCCGGACG 
CCACCTACGC GATCGCTTTC CATGCGGGAG CGACGTCAGC TGGACTGCAG TCCCATACCG 
CGTAGTCAGT CAGGAGCCTC TCCCGCCTCC ATATCGAGCT CGACAGTCAG CACATCGGCT 
CTGGCCTCAC ATCCACATGT TAATCTGCTG CACGCGGCAG AGCCACATTA TTATAATGCT 
CAGGCACTGC CCCAGGGAGC TGCTTACTAC ACTAGTTACC ATGGATCACC GCACGACTTG 
AGCTATGCCA GTTCGGCGGA CACCTCGCTA AATGCCTCGT GGGTTAACAC GAGCGGCCAC 
TCCCCGCACA CGCCCTACTA TTCGGCGGCC CAGATCTATA TGCGACCCAA GGGCGGCAGC 
ACCACGCCCA CGCCCAGTTG CAGTGGCAGC ACAGGTAGCG GCAGCGGAGG CAGCGGGAGT 
GGCAGTAGCA AGAAGGTGCC ACCAGAGGTG CCCAAACGCA CCAGTTCCAT TACGGCACAA 
CAGCAGACAC AGCTTCTTTT GCTGCAGCGC CAGACACCGC CGCCTCCTTC GCTGCTAAGG 
ACCAATGGCC TGTGCAAAAC CGCCGAGAAC GGCAGTCTGA CCTCCGTGCA GAGTTCCGGA 
TCGGATTCTA GTGTTACCTC TGCGGAACGC AATCTAAACA GCGATTTGGG CTCGGATCGC 
AGTAACTCAC CACATACATG GAAACGAGGA ACAGCCCTAA ATAGTTCCCA GCAGTTCTCC 
ACGCACTCGG CGGATTCAGC GGGTGCGGTG TCTGGTGGAG GAGTTGGAGT GGCTGGAGGA 
GCCGGCGTTT ATGCCGCTCA AATGCAGGCT GCCGTTGCAG CAGCTACAGC GGCAGGAGGA 
ATGCCACCAG CGGATGACCA TGCTATCTCC TCGCATACGA GTGCCGCTCA GTATGAGCAG 
CATGAGCAGC AGCAGCACGA GCAACAGCAA TTGCAGGCGG CAGCTGCAGC TGCCGGAGTG 
GCACAAAACT ACAAGATGTC GGAGACGATA CGCAAGCGAC AGTATCGCGT TGGACTCAAT 
CTGTTCAACA AGAAGCCGGA GAAGGGCATC ACCTATCTGA TCAGGCGGGG ATTCCTTGAG 
AATACACCAC AGGGCGTTGC TCGTTTCCTC ATCACCCGTA AGGGTTTGTC CCGGCAAATG 
ATTGGCGAGT ATCTGGGAAA TTTGCAGAAC CAGTTCAACA TGGCCGTGCT CAGTTGCTTT 
GCCATGGAGC TGGACCTGTC CGGCCGGCAA GTGGATGTGG CTTTGCGAAA GTTCCAGGCC 
TATTTCCGCA TGCCTGGAGA GGCACAAAAG ATTGAGCGCC TCATGGAGAT CTTTTCACAG 
CGCTATTGTG AATGCAATGC GGACATTGTC GGGCGATTGA GATCATCCGA TACGATCTTC 
GTCCTGGCTT TCGCCATCAT CATGCTGAAC ACGGATCTGC ACACACCCAA TCTAAAGCCA 
GAACGTCGCA TGCGCGTCGA GGACTTTATC AAGAATCTGC GCGGCATCGA CGACTGTCAC 
GACATTGATA AGGACATGCT GATGGGCATC TATGACCGTG TCAAGTCCGA CGAATTTAAG 
CCCGGCAGCG ACCATGTCAC TCAAGTGATG AAGGTACAGG CCACTATTGT GGGCAAGAAA 
CCAAATCTAG CGCTGCCCCA TCGGCGTCTT GTCTGCTATT GCCGACTGTA CGAGATTCCT 
GACGTGAACA AGAAGGAGCG ACCTGGTGTG CATCAGCGCG AGGTGTTTCT GTTCAACGAT 
CTGCTGGTCA TTACCAAAAT ATTTAGCAAA AAGAAGACCT CCGTGACGTA CACATTCCGC 
AACAGTTTCC CGCTATGCGG CACCGTTGTC ACCCTTCTGG ACATGCCCAA CTATCCGTTT 
TGCATTCAGC TCTCCCAGAA AGTGGATGGC AAGATCTTGA TCACCTTCAA CGCCCGCAAC 
GAACACGATC GCTGCAAGTT TGCCGAGGAT CTTAAAGAGT CCATTAGCGA GATGGACGAG 
ATGGAGTCGC TGCGCATTGA GGCCGAACTG GAGCGCCAGA AGTCGGCGCG CAATCGAGCA 
CCTGGCAATG CGGAGAATCG TGACAGTGGC GTAGCCGATG TGGAGGTCTG CCCATGCCCG 
TATCAGCAAG GATCACAAGC ATCTGGCGAG CAGGCTCCAA ACTCTGCCGA TAACTCGCAG 
CAGCTGAAGC GCAGTGCGCT GAGCAACAGC CTTCTCGACA TGCACGAGCA GTTTGGCAAT 
GAGAAACCTC AACGTCGTGG CAGCGTTGGC TCCCTGGACA GCGGCATGAG CATCTCGTTC 
CAGTCCACTA CAACATCCAG CGCTTCGAGG GAAAATGCCG CTGCCATTGC GGCCGCAGCA 
AATGCAGCTG CAGCAGCCAA GATGCGATTT AACATGCCGC CAACGGCAGC GATTGCCACG 
CCCAGCAATG TGTATGCAGC TCCGGGAATG CAGGCGTATA CCCATGCCAA CTTTGTGCAG 
CAGTCACAGG CCGCCTACAT GTTGCAGCAA CAGCAAATGC TCCAGCAGCA GGCACAAATG 
CAAGCTCAGG CTCAGGCTCA AGCCCAAGCT CAGGCCCAAG CTCAAGCCCA GGCTCAGGCG 
CAGGCGCAGG CGCAGCCGCT TACTGGCCGA ATACCGGGAC GCGAGCGAAA GGCTTCGCGG 
ACGGATGAGA ACGGACGGTC GACGGAGGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036618.1
CDS	254..3886
Translation	MIICISGFIIGESFPQKSLERSGSTQYELAGAQQPGSANASTCTDSGSVGGYVYLQNHYAPGAHSSSAAINYPAQQHPQMVYQIQQYPTCHQQQQQQHLQQQQHLHQTGAGHYMQVTATGGGQYHHHHMLHGHGHHAHHHGGAVVIAGSGVGTGLGSGATSVIMQHQQQQQQQQNMHKKNSIRNGGDVLKRTRAQSAYELSQDLLEKQIELLERKYGGVRARNAAVTIQRAFRHYMMVKKFASITAMAKAEKRLSRRMVVTASSLGLAEEGASSSSAYGSATESQLTEQQQQQQAQQQQQPRVTIMAGPAGAASPGLSRTPPTRSLSMRERRQLDCSPIPRSQSGASPASISSSTVSTSALASHPHVNLLHAAEPHYYNAQALPQGAAYYTSYHGSPHDLSYASSADTSLNASWVNTSGHSPHTPYYSAAQIYMRPKGGSTTPTPSCSGSTGSGSGGSGSGSSKKVPPEVPKRTSSITAQQQTQLLLLQRQTPPPPSLLRTNGLCKTAENGSLTSVQSSGSDSSVTSAERNLNSDLGSDRSNSPHTWKRGTALNSSQQFSTHSADSAGAVSGGGVGVAGGAGVYAAQMQAAVAAATAAGGMPPADDHAISSHTSAAQYEQHEQQQHEQQQLQAAAAAAGVAQNYKMSETIRKRQYRVGLNLFNKKPEKGITYLIRRGFLENTPQGVARFLITRKGLSRQMIGEYLGNLQNQFNMAVLSCFAMELDLSGRQVDVALRKFQAYFRMPGEAQKIERLMEIFSQRYCECNADIVGRLRSSDTIFVLAFAIIMLNTDLHTPNLKPERRMRVEDFIKNLRGIDDCHDIDKDMLMGIYDRVKSDEFKPGSDHVTQVMKVQATIVGKKPNLALPHRRLVCYCRLYEIPDVNKKERPGVHQREVFLFNDLLVITKIFSKKKTSVTYTFRNSFPLCGTVVTLLDMPNYPFCIQLSQKVDGKILITFNARNEHDRCKFAEDLKESISEMDEMESLRIEAELERQKSARNRAPGNAENRDSGVADVEVCPCPYQQGSQASGEQAPNSADNSQQLKRSALSNSLLDMHEQFGNEKPQRRGSVGSLDSGMSISFQSTTTSSASRENAAAIAAAANAAAAAKMRFNMPPTAAIATPSNVYAAPGMQAYTHANFVQQSQAAYMLQQQQMLQQQAQMQAQAQAQAQAQAQAQAQAQAQAQAQPLTGRIPGRERKASRTDENGRSTEV

Target ORF information:

RefSeq Version	NM_001043153.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster schizo (siz), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043153.2

ORF Insert Sequence:

ATGATCATTT GCATATCGGG TTTTATAATT GGCGAAAGCT TTCCCCAGAA GAGCCTGGAG 
CGAAGTGGTT CCACCCAGTA TGAGCTGGCT GGAGCGCAAC AGCCAGGATC AGCCAACGCC 
TCCACCTGCA CAGATAGCGG CAGTGTTGGC GGCTATGTTT ACCTGCAGAA TCACTACGCC 
CCCGGCGCCC ACAGCTCCTC AGCCGCCATC AACTACCCTG CGCAACAGCA TCCCCAGATG 
GTCTACCAAA TACAGCAGTA TCCCACGTGC CATCAGCAGC AGCAACAGCA GCACCTCCAG 
CAGCAGCAGC ATTTGCATCA GACCGGCGCA GGTCACTACA TGCAGGTGAC GGCGACGGGT 
GGTGGCCAGT ATCATCACCA TCACATGCTC CATGGTCACG GGCATCATGC CCACCATCAC 
GGTGGGGCGG TGGTCATCGC CGGGAGTGGT GTGGGAACTG GCCTGGGATC TGGAGCCACC 
AGCGTGATCA TGCAGCACCA GCAACAGCAG CAACAGCAAC AGAATATGCA CAAGAAGAAC 
TCCATCCGGA ACGGCGGAGA TGTCCTCAAG CGAACGCGAG CTCAGTCGGC CTACGAACTC 
TCACAAGATC TGCTTGAGAA ACAGATCGAG CTGCTGGAGC GCAAGTACGG CGGAGTGCGA 
GCCCGTAACG CAGCGGTCAC TATTCAGCGC GCCTTCCGTC ACTACATGAT GGTCAAGAAG 
TTCGCCTCGA TCACGGCAAT GGCCAAAGCT GAGAAGCGTC TAAGCCGGCG GATGGTGGTT 
ACAGCCAGCA GTCTTGGATT GGCGGAGGAG GGTGCCTCCT CCTCGTCAGC CTACGGAAGT 
GCCACGGAAT CTCAGCTCAC CGAGCAGCAA CAGCAACAAC AGGCGCAGCA GCAACAGCAG 
CCACGTGTCA CCATCATGGC GGGTCCGGCG GGAGCAGCTT CTCCGGGCTT ATCCCGGACG 
CCACCTACGC GATCGCTTTC CATGCGGGAG CGACGTCAGC TGGACTGCAG TCCCATACCG 
CGTAGTCAGT CAGGAGCCTC TCCCGCCTCC ATATCGAGCT CGACAGTCAG CACATCGGCT 
CTGGCCTCAC ATCCACATGT TAATCTGCTG CACGCGGCAG AGCCACATTA TTATAATGCT 
CAGGCACTGC CCCAGGGAGC TGCTTACTAC ACTAGTTACC ATGGATCACC GCACGACTTG 
AGCTATGCCA GTTCGGCGGA CACCTCGCTA AATGCCTCGT GGGTTAACAC GAGCGGCCAC 
TCCCCGCACA CGCCCTACTA TTCGGCGGCC CAGATCTATA TGCGACCCAA GGGCGGCAGC 
ACCACGCCCA CGCCCAGTTG CAGTGGCAGC ACAGGTAGCG GCAGCGGAGG CAGCGGGAGT 
GGCAGTAGCA AGAAGGTGCC ACCAGAGGTG CCCAAACGCA CCAGTTCCAT TACGGCACAA 
CAGCAGACAC AGCTTCTTTT GCTGCAGCGC CAGACACCGC CGCCTCCTTC GCTGCTAAGG 
ACCAATGGCC TGTGCAAAAC CGCCGAGAAC GGCAGTCTGA CCTCCGTGCA GAGTTCCGGA 
TCGGATTCTA GTGTTACCTC TGCGGAACGC AATCTAAACA GCGATTTGGG CTCGGATCGC 
AGTAACTCAC CACATACATG GAAACGAGGA ACAGCCCTAA ATAGTTCCCA GCAGTTCTCC 
ACGCACTCGG CGGATTCAGC GGGTGCGGTG TCTGGTGGAG GAGTTGGAGT GGCTGGAGGA 
GCCGGCGTTT ATGCCGCTCA AATGCAGGCT GCCGTTGCAG CAGCTACAGC GGCAGGAGGA 
ATGCCACCAG CGGATGACCA TGCTATCTCC TCGCATACGA GTGCCGCTCA GTATGAGCAG 
CATGAGCAGC AGCAGCACGA GCAACAGCAA TTGCAGGCGG CAGCTGCAGC TGCCGGAGTG 
GCACAAAACT ACAAGATGTC GGAGACGATA CGCAAGCGAC AGTATCGCGT TGGACTCAAT 
CTGTTCAACA AGAAGCCGGA GAAGGGCATC ACCTATCTGA TCAGGCGGGG ATTCCTTGAG 
AATACACCAC AGGGCGTTGC TCGTTTCCTC ATCACCCGTA AGGGTTTGTC CCGGCAAATG 
ATTGGCGAGT ATCTGGGAAA TTTGCAGAAC CAGTTCAACA TGGCCGTGCT CAGTTGCTTT 
GCCATGGAGC TGGACCTGTC CGGCCGGCAA GTGGATGTGG CTTTGCGAAA GTTCCAGGCC 
TATTTCCGCA TGCCTGGAGA GGCACAAAAG ATTGAGCGCC TCATGGAGAT CTTTTCACAG 
CGCTATTGTG AATGCAATGC GGACATTGTC GGGCGATTGA GATCATCCGA TACGATCTTC 
GTCCTGGCTT TCGCCATCAT CATGCTGAAC ACGGATCTGC ACACACCCAA TCTAAAGCCA 
GAACGTCGCA TGCGCGTCGA GGACTTTATC AAGAATCTGC GCGGCATCGA CGACTGTCAC 
GACATTGATA AGGACATGCT GATGGGCATC TATGACCGTG TCAAGTCCGA CGAATTTAAG 
CCCGGCAGCG ACCATGTCAC TCAAGTGATG AAGGTACAGG CCACTATTGT GGGCAAGAAA 
CCAAATCTAG CGCTGCCCCA TCGGCGTCTT GTCTGCTATT GCCGACTGTA CGAGATTCCT 
GACGTGAACA AGAAGGAGCG ACCTGGTGTG CATCAGCGCG AGGTGTTTCT GTTCAACGAT 
CTGCTGGTCA TTACCAAAAT ATTTAGCAAA AAGAAGACCT CCGTGACGTA CACATTCCGC 
AACAGTTTCC CGCTATGCGG CACCGTTGTC ACCCTTCTGG ACATGCCCAA CTATCCGTTT 
TGCATTCAGC TCTCCCAGAA AGTGGATGGC AAGATCTTGA TCACCTTCAA CGCCCGCAAC 
GAACACGATC GCTGCAAGTT TGCCGAGGAT CTTAAAGAGT CCATTAGCGA GATGGACGAG 
ATGGAGTCGC TGCGCATTGA GGCCGAACTG GAGCGCCAGA AGTCGGCGCG CAATCGAGCA 
CCTGGCAATG CGGAGAATCG TGACAGTGGC GTAGCCGATG TGGAGGTCTG CCCATGCCCG 
TATCAGCAAG GATCACAAGC ATCTGGCGAG CAGGCTCCAA ACTCTGCCGA TAACTCGCAG 
CAGCTGAAGC GCAGTGCGCT GAGCAACAGC CTTCTCGACA TGCACGAGCA GTTTGGCAAT 
GAGAAACCTC AACGTCGTGG CAGCGTTGGC TCCCTGGACA GCGGCATGAG CATCTCGTTC 
CAGTCCACTA CAACATCCAG CGCTTCGAGG GAAAATGCCG CTGCCATTGC GGCCGCAGCA 
AATGCAGCTG CAGCAGCCAA GATGCGATTT AACATGCCGC CAACGGCAGC GATTGCCACG 
CCCAGCAATG TGTATGCAGC TCCGGGAATG CAGGCGTATA CCCATGCCAA CTTTGTGCAG 
CAGTCACAGG CCGCCTACAT GTTGCAGCAA CAGCAAATGC TCCAGCAGCA GGCACAAATG 
CAAGCTCAGG CTCAGGCTCA AGCCCAAGCT CAGGCCCAAG CTCAAGCCCA GGCTCAGGCG 
CAGGCGCAGG CGCAGCCGCT TACTGGCCGA ATACCGGGAC GCGAGCGAAA GGCTTCGCGG 
ACGGATGAGA ACGGACGGTC GACGGAGGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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siz cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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