Mst77F cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mst77F
Entrez Gene ID	40304
Full Name	Male-specific transcript 77F
Synonyms	18c,CG3354,Dmel\CG3354,MST77F,R1-18c,ms(3)nc3,mst77F,nc3
General protein information	Preferred Names Male-specific transcript 77F Names CG3354 gene product from transcript CG3354-RA CG3354-PA Mst77F-PA male sterile (3) nc3
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 77E-77F

mRNA and Protein(s)

mRNA	Protein	Name
NM_079464.3	NP_524188.1	Male-specific transcript 77F

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data demonstrate that Mst77F is required for the proper compaction of spermatid chromatin following the histone-to-protamine transition, and show that Mst77F is proteolytically processed during spermiogenesis, in a way remarkably similar to mammalian P2.
Title: The Drosophila chromosomal protein Mst77F is processed to generate an essential component of mature sperm chromatin.

An intrinsically unstructured domain in the C-terminus of Mst77F binds DNA via electrostatic interaction. This binding results in structural reorganization of the domain, which induces interaction with an N-terminal region of the protein.
Title: Multimerization of Drosophila sperm protein Mst77F causes a unique condensed chromatin structure.

Expression of Mst77F, which is a specialized linker histone-like component of sperm chromatin, and of protamine B (ProtB), which contributes to formation of condensed sperm chromatin, is regulated at three levels.
Title: Three levels of regulation lead to protamine and Mst77F expression in Drosophila.

Spermatids of ms(3)nc3 (Mst77F(1)) mutants show disturbed nuclear shaping, instability of perinuclear microtubules but no obvious chromatin condensation defects.
Title: Distinct functions of Mst77F and protamines in nuclear shaping and chromatin condensation during Drosophila spermiogenesis.

identification of functional Mst77Y genes
Title: Functional copies of the Mst77F gene on the Y chromosome of Drosophila melanogaster.

The following Mst77F gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mst77F cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa21141	NM_079464.3 Latest version!	Drosophila melanogaster Male-specific transcript 77F (Mst77F), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mst77F ( NM_079464.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524188.1 Drosophila melanogaster Male-specific transcript 77F (Mst77F), mRNA.

Price & Availability↑

CloneID	OFa21141
Clone ID Related Accession (Same CDS sequence)	NM_079464.3
Accession Version	NM_079464.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Male-specific transcript 77F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079464.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTAATC TGAAACAAAA GGATAGCAAG CCGGAGGTGG CAGTAACAAA ATCAGTGAAA 
ACCTATAAAA AATCAATTGA ATATGTTAAT TCCGACGCCT CCGACATTGA AGAAGATATC 
AATAGGGCAG AGGACGAATA CGCCTCATCG TCTGGCTTTG TTAATTTTCT GAGGGACTTT 
AAGAAGCGCT ACGGAGAATA TTACTCGAAT AACGAGATAA GACGGGCTGC TGAAACCCGA 
TGGAACGAAA TGTCATTCCG TCATCGATGC CAGTATTCTG CGGAACCATT GGACACTTTT 
CATGTAGAGC CCAACAGTGT GAGCAGTCTT CAGCGTTCTA GTGAGGGCGA GCACAGAATG 
CACTCTGAAA TAAGTGGCTG CGCAGACACT TTCTTCGGTG CCGGTGGCTC CAATAGCTGC 
ACTCCAAGAA AGGAGAACAA GTGTTCCAAG CCCAGGGTGC GGAAGAGTTG CCCCAAACCA 
CGGGCGAAAA CCTCGAAGCA ACGTCGCAGT TGCGGCAAAC CGAAGCCCAA GGGCGCCCGA 
CCCCGGAAGG CATGTCCCCG CCCCAGGAAG AAGATGGAAT GCGGCAAGGC GAAGGCAAAG 
CCAAGGTGTC TTAAGCCCAA GAGCTCCAAG CCCAAGTGCT CGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524188.1
CDS	150..797
Translation	MSNLKQKDSKPEVAVTKSVKTYKKSIEYVNSDASDIEEDINRAEDEYASSSGFVNFLRDFKKRYGEYYSNNEIRRAAETRWNEMSFRHRCQYSAEPLDTFHVEPNSVSSLQRSSEGEHRMHSEISGCADTFFGAGGSNSCTPRKENKCSKPRVRKSCPKPRAKTSKQRRSCGKPKPKGARPRKACPRPRKKMECGKAKAKPRCLKPKSSKPKCSM

Target ORF information:

RefSeq Version	NM_079464.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Male-specific transcript 77F (Mst77F), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079464.3

ORF Insert Sequence:

ATGAGTAATC TGAAACAAAA GGATAGCAAG CCGGAGGTGG CAGTAACAAA ATCAGTGAAA 
ACCTATAAAA AATCAATTGA ATATGTTAAT TCCGACGCCT CCGACATTGA AGAAGATATC 
AATAGGGCAG AGGACGAATA CGCCTCATCG TCTGGCTTTG TTAATTTTCT GAGGGACTTT 
AAGAAGCGCT ACGGAGAATA TTACTCGAAT AACGAGATAA GACGGGCTGC TGAAACCCGA 
TGGAACGAAA TGTCATTCCG TCATCGATGC CAGTATTCTG CGGAACCATT GGACACTTTT 
CATGTAGAGC CCAACAGTGT GAGCAGTCTT CAGCGTTCTA GTGAGGGCGA GCACAGAATG 
CACTCTGAAA TAAGTGGCTG CGCAGACACT TTCTTCGGTG CCGGTGGCTC CAATAGCTGC 
ACTCCAAGAA AGGAGAACAA GTGTTCCAAG CCCAGGGTGC GGAAGAGTTG CCCCAAACCA 
CGGGCGAAAA CCTCGAAGCA ACGTCGCAGT TGCGGCAAAC CGAAGCCCAA GGGCGCCCGA 
CCCCGGAAGG CATGTCCCCG CCCCAGGAAG AAGATGGAAT GCGGCAAGGC GAAGGCAAAG 
CCAAGGTGTC TTAAGCCCAA GAGCTCCAAG CCCAAGTGCT CGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Mst77F cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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