Eip75B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eip75B
Entrez Gene ID	39999
Full Name	Ecdysone-induced protein 75B
Synonyms	57B,75B,BcDNA:GM02640,CG8127,DmE75A,DmE75B,Dmel\CG8127,E75,E75-C,E75A,E75B,E75C,EP1121b,Eip75,Eip75 A,Eip75A,Mgn00274,NR1D3,Rev-Erb,anon-WO0172774.31,anon-WO0172774.32,dE75,eip75B,l(3)07041,l(3)j11A6,l(3)j12E8,l(3)j3A6,l(3)j5E1,l(3)neo25
General protein information	Preferred Names Ecdysone-induced protein 75B Names CG8127 gene product from transcript CG8127-RF CG8127-PA CG8127-PB CG8127-PC CG8127-PD CG8127-PE CG8127-PF CG8127-PG E75 nuclear receptor Eip75B-PA Eip75B-PB Eip75B-PC Eip75B-PD Eip75B-PE Eip75B-PF Eip75B-PG ecdysone induced protein 75 ecdysone inducible protein 75B ecdysone-induced protein 75
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 75A10-75B6

mRNA and Protein(s)

mRNA	Protein	Name
NM_168757.1	NP_730323.1	Ecdysone-induced protein 75B, isoform D
NM_001259893.2	NP_001246822.1	Ecdysone-induced protein 75B, isoform F
NM_168755.1	NP_730321.1	Ecdysone-induced protein 75B, isoform B
NM_168756.2	NP_730322.1	Ecdysone-induced protein 75B, isoform C
NM_079409.3	NP_524133.2	Ecdysone-induced protein 75B, isoform A
NM_001259892.1	NP_001246821.1	Ecdysone-induced protein 75B, isoform E
NM_001316451.1	NP_001303380.1	Ecdysone-induced protein 75B, isoform G

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012223	XP_320316.4
Drosophila melanogaster (fruit fly)	Eip75B	NP_730322.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that E75C, like UNF, is required for developmental regrowth, but not initial outgrowth of mushroom body gamma axons.
Title: Nitric Oxide as a Switching Mechanism between Axon Degeneration and Regrowth during Developmental Remodeling.

E75 and UNF bind to PER regulatory sequences and act together to enhance the CLK/CYC-mediated transcription of the PER gene.
Title: Transcriptional regulation via nuclear receptor crosstalk required for the Drosophila circadian clock.

E75 and ecdysone receptor are expressed in central clock cells, and manipulations of their expression produce drastic effects on circadian rhythms in Drosophila.
Title: An ecdysone-responsive nuclear receptor regulates circadian rhythms in Drosophila.

Covalent attachment of heme to the protein moiety in an insect E75 nitric oxide sensor.
Title: Covalent attachment of heme to the protein moiety in an insect E75 nitric oxide sensor.

Ecdysone and NO mediate gene regulation by competing EcR/Usp and E75A nuclear receptors during Drosophila development
Title: Ecdysone- and NO-mediated gene regulation by competing EcR/Usp and E75A nuclear receptors during Drosophila development.

The following Eip75B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eip75B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18848	NM_001259892.1 Latest version!	Drosophila melanogaster Ecdysone-induced protein 75B (Eip75B), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OFa18848	NM_001259893.2 Latest version!	Drosophila melanogaster Ecdysone-induced protein 75B (Eip75B), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OFa18848	NM_168755.1 Latest version!	Drosophila melanogaster Ecdysone-induced protein 75B (Eip75B), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OFa21052	NM_168756.2 Latest version!	Drosophila melanogaster Ecdysone-induced protein 75B (Eip75B), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa21053	NM_079409.3 Latest version!	Drosophila melanogaster Ecdysone-induced protein 75B (Eip75B), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OFa21054	NM_168757.1 Latest version!	Drosophila melanogaster Ecdysone-induced protein 75B (Eip75B), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa41376	NM_001316451.1 Latest version!	Drosophila melanogaster Ecdysone-induced protein 75B (Eip75B), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eip75B ( NM_001259893.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246822.1 Drosophila melanogaster Ecdysone-induced protein 75B (Eip75B), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa18848
Clone ID Related Accession (Same CDS sequence)	NM_001259893.2 , NM_168755.1 , NM_001259892.1
Accession Version	NM_001259893.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ecdysone-induced protein 75B, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001259893.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGCGG TCCAGGCAGC AGCGGCTGCA ACATCATCCG GAGGATCTTC AGGCAGTGTT 
CCGGGTTCCG GTTCCGGATC TGCATCGAAA CTGATCAAGA CCGAGCCCAT TGACTTTGAG 
ATGCTGCATC TGGAGGAGAA CGAACGGCAG CAGGATATCG AGCGGGAGCC GAGCAGCAGC 
AATAGCAACA GCAATAGCAA CAGTTTGACC CCCCAGCGAT ACACACACGT GCAGGTGCAG 
ACCGTGCCAC CACGCCAACC CACAGGTCTG ACCACGCCCG GTGGCACCCA GAAGGTCATT 
CTGACTCCTC GCGTAGAGTA CGTGCAACAG CGAGCCACCA GTTCCACAGG TGGTGGGATG 
AAGCACGTAT ACAGCCAACA GCAGGGCACT GCTGCATCGC GATCCGCTCC ACCCGAGACC 
ACGGCCCTAC TGACCACCAC TTCGGGCACA CCACAGATTA TCATCACTCG AACCCTACCC 
TCCAATCAGC ACCTGTCGCG CCGTCATTCG GCCAGTCCCT CCGCCCTGCA CCACTACCAG 
CAGCAGCAGC CGCAGCGTCA GCAATCGCCG CCACCGCTGC ACCACCAACA GCAGCAGCAG 
CAGCAGCATG TCCGCGTGAT CCGCGATGGC CGGCTGTACG ATGAAGCCAC GGTAGTGGTG 
GCTGCCCGTC GGCATTCGGT GTCGCCACCA CCACTGCACC ACCACTCCAG ATCGGCGCCC 
GTGTCTCCGG TGATCGCCAG GCGCGGTGGT GCTGCCGCAT ATATGGATCA GCAATATCAG 
CAACGGCAGA CGCCACCGCT GGCACCACCG CCGCCGCCTC CACCACCGCC GCCACCACCA 
CCGCCACCCC AACAGCAGCA GCAACAGTAC ATCTCCACTG GTGTTCCACC ACCCACGGCA 
GCGGCTCGGA AGTTTGTGGT TAGCACCAGC ACGCGACACG TTAATGTGAT CGCCTCCAAT 
CACTTTCAAC AACAGCAGCA GCAGCACCAA GCACAGCAGC ATCAGCAGCA GCATCAGCAG 
CATCAGCAAC ATCAGCAGCA TGTGATCGCC AGCGTTAGTT CTAGCAGCAG CTCATCGGCC 
ATTGGATCAG GCGGCTCTTC TTCTTCGCAC ATCTTTCGCA CGCCCGTGGT TTCCAGCAGC 
AGCAGCAGCA ATATGCACCA TCAGCAGCAA CAGCAACAGC AGCAATCGAG CCTGGGCAAC 
TCGGTGATGC GGCCACCTCC GCCGCCGCCT CCGCCCAAGG TCAAACACGC CTCCAGCTCC 
AGCAGCGGCA ACTCGAGCAG CAGCAACACC AACAACAGCA GCAGCAGCAG CAACGGCGAG 
GAGCCGTCTA GCTCGATTCC TGATCTAGAA TTCGATGGCA CCACAGTGCT GTGCCGCGTT 
TGCGGGGATA AGGCCTCCGG TTTCCATTAC GGCGTGCATT CCTGCGAGGG TTGCAAGGGA 
TTCTTCCGCC GCTCCATCCA GCAAAAGATC CAGTATCGCC CGTGCACCAA GAATCAGCAG 
TGCAGCATTC TGCGCATCAA TCGCAATCGT TGTCAATATT GCCGCCTGAA AAAGTGCATT 
GCCGTGGGCA TGAGTCGCGA TGCTGTGCGT TTTGGACGCG TGCCGAAGCG CGAAAAGGCG 
CGTATCCTGG CGGCCATGCA ACAGAGCACC CAGAATCGCG GCCAGCAGCG AGCCCTCGCC 
ACCGAGCTGG ATGACCAGCC ACGCCTCCTC GCCGCCGTGC TGCGCGCCCA CCTCGAGACC 
TGTGAGTTCA CCAAGGAGAA GGTCTCGGCG ATGCGGCAGC GGGCGCGGGA TTGCCCCTCC 
TACTCCATGC CCACACTTCT GGCCTGTCCG CTGAACCCCG CCCCTGAACT GCAATCGGAG 
CAGGAGTTCT CGCAGCGTTT CGCCCACGTA ATTCGCGGCG TGATCGACTT TGCCGGCATG 
ATTCCCGGCT TCCAGCTGCT CACCCAGGAC GATAAGTTCA CGCTCCTGAA GGCGGGACTC 
TTCGACGCCC TGTTTGTGCG CCTGATCTGC ATGTTTGACT CGTCGATAAA CTCAATCATC 
TGTCTAAATG GCCAGGTGAT GCGACGGGAT GCGATCCAGA ACGGAGCCAA TGCCCGCTTC 
CTGGTGGACT CCACCTTCAA TTTCGCGGAG CGCATGAACT CGATGAACCT GACAGATGCC 
GAGATAGGCC TGTTCTGCGC CATCGTTCTG ATTACGCCGG ATCGCCCCGG TTTGCGCAAC 
CTGGAGCTGA TCGAGAAGAT GTACTCGCGA CTCAAGGGCT GCCTGCAGTA CATTGTCGCC 
CAGAATAGGC CCGATCAGCC CGAGTTCCTG GCCAAGTTGC TGGAGACGAT GCCCGATCTG 
CGCACCCTGA GCACCCTGCA CACCGAGAAA CTGGTAGTTT TCCGCACCGA GCACAAGGAG 
CTGCTGCGCC AGCAGATGTG GTCCATGGAG GACGGCAACA ACAGCGATGG CCAGCAGAAC 
AAGTCGCCCT CGGGCAGCTG GGCGGATGCC ATGGACGTGG AGGCGGCCAA GAGTCCGCTT 
GGCTCGGTAT CGAGCACTGA GTCCGCCGAC CTGGACTACG GCAGTCCGAG CAGTTCGCAG 
CCACAGGGCG TGTCTCTGCC CTCGCCGCCT CAGCAACAGC CCTCGGCTCT GGCCAGCTCG 
GCTCCTCTGC TGGCGGCCAC CCTCTCCGGA GGATGTCCCC TGCGCAACCG GGCCAATTCC 
GGCTCCAGCG GTGACTCCGG AGCAGCTGAG ATGGATATCG TTGGCTCGCA CGCACATCTC 
ACCCAGAACG GGCTGACAAT CACGCCGATT GTGCGACACC AGCAGCAGCA ACAACAGCAG 
CAGCAGATCG GAATACTCAA TAATGCGCAT TCCCGCAACT TGAATGGGGG ACACGCGATG 
TGCCAGCAAC AGCAGCAGCA CCCACAACTG CACCACCACT TGACAGCCGG AGCTGCCCGC 
TACAGAAAGC TAGATTCGCC CACGGATTCG GGCATTGAGT CGGGCAACGA GAAGAACGAG 
TGCAAGGCGG TGAGTTCGGG GGGAAGTTCC TCGTGCTCCA GTCCGCGTTC CAGTGTGGAT 
GATGCGCTGG ACTGCAGCGA TGCCGCCGCC AATCACAATC AGGTGGTGCA GCATCCGCAG 
CTGAGTGTGG TGTCCGTGTC ACCAGTTCGC TCGCCCCAGC CCTCCACCAG CAGCCATCTG 
AAGCGACAGA TTGTGGAGGA TATGCCCGTG CTGAAGCGCG TGCTGCAGGC TCCCCCTCTG 
TACGATACCA ACTCGCTGAT GGACGAGGCC TACAAGCCGC ACAAGAAATT CCGGGCCCTG 
CGGCATCGCG AGTTCGAGAC CGCCGAGGCG GATGCCAGCA GTTCCACTTC CGGCTCGAAC 
AGCCTGAGTG CCGGCAGTCC GCGACAGAGT CCAGTCCCGA ACAGTGTGGC CACGCCCCCG 
CCATCGGCGG CCAGCGCCGC CGCAGGTAAT CCCGCCCAGA GCCAGCTGCA CATGCACCTG 
ACCCGCAGCA GCCCCAAGGC CTCGATGGCC AGCTCGCACT CGGTGCTGGC CAAGTCTCTC 
ATGGCCGAGC CGCGCATGAC GCCCGAGCAG ATGAAGCGCA GCGATATTAT CCAAAACTAC 
TTGAAGCGCG AGAACAGCAC AGCAGCCAGC AGCACCACCA ATGGCGTGGG CAACCGCAGT 
CCCAGCAGCA GCTCCACACC GCCGCCATCG GCGGTCCAGA ATCAGCAGCG TTGGGGCAGC 
AGCTCGGTGA TCACCACCAC CTGCCAGCAG CGCCAGCAGT CCGTGTCGCC GCACAGCAAC 
GGTTCCAGCT CCAGTTCGAG CTCTAGCTCC AGCTCCAGTT CGTCATCCTC CTCCACATCC 
TCCAACTGCA GCTCCAGCTC GGCCAGCAGC TGCCAGTATT TCCAGTCGCC GCACTCCACC 
AGCAACGGCA CCAGTGCACC GGCGAGCTCC AGTTCGGGAT CGAACAGCGC CACGCCCCTG 
CTGGAACTGC AGGTGGACAT TGCTGACTCG GCGCAGCCTC TCAATTTGTC CAAGAAATCG 
CCCACGCCGC CGCCCAGCAA GCTGCACGCT CTGGTGGCCG CCGCCAATGC CGTTCAAAGG 
TATCCCACAT TGTCCGCCGA CGTCACAGTG ACAGCCTCCA ATGGCGGTCC TCCGTCGGCG 
GCGGCGAGTC CGGCGCCCAG CAGCAGTCCG CCGGCGAGTG TGGGCTCCCC CAATCCGGGC 
CTGAGCGCCG CCGTGCACAA GGTAATGCTG GAGGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246822.1
CDS	1310..5548
Translation	MEAVQAAAAATSSGGSSGSVPGSGSGSASKLIKTEPIDFEMLHLEENERQQDIEREPSSSNSNSNSNSLTPQRYTHVQVQTVPPRQPTGLTTPGGTQKVILTPRVEYVQQRATSSTGGGMKHVYSQQQGTAASRSAPPETTALLTTTSGTPQIIITRTLPSNQHLSRRHSASPSALHHYQQQQPQRQQSPPPLHHQQQQQQQHVRVIRDGRLYDEATVVVAARRHSVSPPPLHHHSRSAPVSPVIARRGGAAAYMDQQYQQRQTPPLAPPPPPPPPPPPPPPPQQQQQQYISTGVPPPTAAARKFVVSTSTRHVNVIASNHFQQQQQQHQAQQHQQQHQQHQQHQQHVIASVSSSSSSSAIGSGGSSSSHIFRTPVVSSSSSSNMHHQQQQQQQQSSLGNSVMRPPPPPPPPKVKHASSSSSGNSSSSNTNNSSSSSNGEEPSSSIPDLEFDGTTVLCRVCGDKASGFHYGVHSCEGCKGFFRRSIQQKIQYRPCTKNQQCSILRINRNRCQYCRLKKCIAVGMSRDAVRFGRVPKREKARILAAMQQSTQNRGQQRALATELDDQPRLLAAVLRAHLETCEFTKEKVSAMRQRARDCPSYSMPTLLACPLNPAPELQSEQEFSQRFAHVIRGVIDFAGMIPGFQLLTQDDKFTLLKAGLFDALFVRLICMFDSSINSIICLNGQVMRRDAIQNGANARFLVDSTFNFAERMNSMNLTDAEIGLFCAIVLITPDRPGLRNLELIEKMYSRLKGCLQYIVAQNRPDQPEFLAKLLETMPDLRTLSTLHTEKLVVFRTEHKELLRQQMWSMEDGNNSDGQQNKSPSGSWADAMDVEAAKSPLGSVSSTESADLDYGSPSSSQPQGVSLPSPPQQQPSALASSAPLLAATLSGGCPLRNRANSGSSGDSGAAEMDIVGSHAHLTQNGLTITPIVRHQQQQQQQQQIGILNNAHSRNLNGGHAMCQQQQQHPQLHHHLTAGAARYRKLDSPTDSGIESGNEKNECKAVSSGGSSSCSSPRSSVDDALDCSDAAANHNQVVQHPQLSVVSVSPVRSPQPSTSSHLKRQIVEDMPVLKRVLQAPPLYDTNSLMDEAYKPHKKFRALRHREFETAEADASSSTSGSNSLSAGSPRQSPVPNSVATPPPSAASAAAGNPAQSQLHMHLTRSSPKASMASSHSVLAKSLMAEPRMTPEQMKRSDIIQNYLKRENSTAASSTTNGVGNRSPSSSSTPPPSAVQNQQRWGSSSVITTTCQQRQQSVSPHSNGSSSSSSSSSSSSSSSSSTSSNCSSSSASSCQYFQSPHSTSNGTSAPASSSSGSNSATPLLELQVDIADSAQPLNLSKKSPTPPPSKLHALVAAANAVQRYPTLSADVTVTASNGGPPSAAASPAPSSSPPASVGSPNPGLSAAVHKVMLEA

Target ORF information:

RefSeq Version	NM_001259893.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ecdysone-induced protein 75B (Eip75B), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259893.2

ORF Insert Sequence:

ATGGAGGCGG TCCAGGCAGC AGCGGCTGCA ACATCATCCG GAGGATCTTC AGGCAGTGTT 
CCGGGTTCCG GTTCCGGATC TGCATCGAAA CTGATCAAGA CCGAGCCCAT TGACTTTGAG 
ATGCTGCATC TGGAGGAGAA CGAACGGCAG CAGGATATCG AGCGGGAGCC GAGCAGCAGC 
AATAGCAACA GCAATAGCAA CAGTTTGACC CCCCAGCGAT ACACACACGT GCAGGTGCAG 
ACCGTGCCAC CACGCCAACC CACAGGTCTG ACCACGCCCG GTGGCACCCA GAAGGTCATT 
CTGACTCCTC GCGTAGAGTA CGTGCAACAG CGAGCCACCA GTTCCACAGG TGGTGGGATG 
AAGCACGTAT ACAGCCAACA GCAGGGCACT GCTGCATCGC GATCCGCTCC ACCCGAGACC 
ACGGCCCTAC TGACCACCAC TTCGGGCACA CCACAGATTA TCATCACTCG AACCCTACCC 
TCCAATCAGC ACCTGTCGCG CCGTCATTCG GCCAGTCCCT CCGCCCTGCA CCACTACCAG 
CAGCAGCAGC CGCAGCGTCA GCAATCGCCG CCACCGCTGC ACCACCAACA GCAGCAGCAG 
CAGCAGCATG TCCGCGTGAT CCGCGATGGC CGGCTGTACG ATGAAGCCAC GGTAGTGGTG 
GCTGCCCGTC GGCATTCGGT GTCGCCACCA CCACTGCACC ACCACTCCAG ATCGGCGCCC 
GTGTCTCCGG TGATCGCCAG GCGCGGTGGT GCTGCCGCAT ATATGGATCA GCAATATCAG 
CAACGGCAGA CGCCACCGCT GGCACCACCG CCGCCGCCTC CACCACCGCC GCCACCACCA 
CCGCCACCCC AACAGCAGCA GCAACAGTAC ATCTCCACTG GTGTTCCACC ACCCACGGCA 
GCGGCTCGGA AGTTTGTGGT TAGCACCAGC ACGCGACACG TTAATGTGAT CGCCTCCAAT 
CACTTTCAAC AACAGCAGCA GCAGCACCAA GCACAGCAGC ATCAGCAGCA GCATCAGCAG 
CATCAGCAAC ATCAGCAGCA TGTGATCGCC AGCGTTAGTT CTAGCAGCAG CTCATCGGCC 
ATTGGATCAG GCGGCTCTTC TTCTTCGCAC ATCTTTCGCA CGCCCGTGGT TTCCAGCAGC 
AGCAGCAGCA ATATGCACCA TCAGCAGCAA CAGCAACAGC AGCAATCGAG CCTGGGCAAC 
TCGGTGATGC GGCCACCTCC GCCGCCGCCT CCGCCCAAGG TCAAACACGC CTCCAGCTCC 
AGCAGCGGCA ACTCGAGCAG CAGCAACACC AACAACAGCA GCAGCAGCAG CAACGGCGAG 
GAGCCGTCTA GCTCGATTCC TGATCTAGAA TTCGATGGCA CCACAGTGCT GTGCCGCGTT 
TGCGGGGATA AGGCCTCCGG TTTCCATTAC GGCGTGCATT CCTGCGAGGG TTGCAAGGGA 
TTCTTCCGCC GCTCCATCCA GCAAAAGATC CAGTATCGCC CGTGCACCAA GAATCAGCAG 
TGCAGCATTC TGCGCATCAA TCGCAATCGT TGTCAATATT GCCGCCTGAA AAAGTGCATT 
GCCGTGGGCA TGAGTCGCGA TGCTGTGCGT TTTGGACGCG TGCCGAAGCG CGAAAAGGCG 
CGTATCCTGG CGGCCATGCA ACAGAGCACC CAGAATCGCG GCCAGCAGCG AGCCCTCGCC 
ACCGAGCTGG ATGACCAGCC ACGCCTCCTC GCCGCCGTGC TGCGCGCCCA CCTCGAGACC 
TGTGAGTTCA CCAAGGAGAA GGTCTCGGCG ATGCGGCAGC GGGCGCGGGA TTGCCCCTCC 
TACTCCATGC CCACACTTCT GGCCTGTCCG CTGAACCCCG CCCCTGAACT GCAATCGGAG 
CAGGAGTTCT CGCAGCGTTT CGCCCACGTA ATTCGCGGCG TGATCGACTT TGCCGGCATG 
ATTCCCGGCT TCCAGCTGCT CACCCAGGAC GATAAGTTCA CGCTCCTGAA GGCGGGACTC 
TTCGACGCCC TGTTTGTGCG CCTGATCTGC ATGTTTGACT CGTCGATAAA CTCAATCATC 
TGTCTAAATG GCCAGGTGAT GCGACGGGAT GCGATCCAGA ACGGAGCCAA TGCCCGCTTC 
CTGGTGGACT CCACCTTCAA TTTCGCGGAG CGCATGAACT CGATGAACCT GACAGATGCC 
GAGATAGGCC TGTTCTGCGC CATCGTTCTG ATTACGCCGG ATCGCCCCGG TTTGCGCAAC 
CTGGAGCTGA TCGAGAAGAT GTACTCGCGA CTCAAGGGCT GCCTGCAGTA CATTGTCGCC 
CAGAATAGGC CCGATCAGCC CGAGTTCCTG GCCAAGTTGC TGGAGACGAT GCCCGATCTG 
CGCACCCTGA GCACCCTGCA CACCGAGAAA CTGGTAGTTT TCCGCACCGA GCACAAGGAG 
CTGCTGCGCC AGCAGATGTG GTCCATGGAG GACGGCAACA ACAGCGATGG CCAGCAGAAC 
AAGTCGCCCT CGGGCAGCTG GGCGGATGCC ATGGACGTGG AGGCGGCCAA GAGTCCGCTT 
GGCTCGGTAT CGAGCACTGA GTCCGCCGAC CTGGACTACG GCAGTCCGAG CAGTTCGCAG 
CCACAGGGCG TGTCTCTGCC CTCGCCGCCT CAGCAACAGC CCTCGGCTCT GGCCAGCTCG 
GCTCCTCTGC TGGCGGCCAC CCTCTCCGGA GGATGTCCCC TGCGCAACCG GGCCAATTCC 
GGCTCCAGCG GTGACTCCGG AGCAGCTGAG ATGGATATCG TTGGCTCGCA CGCACATCTC 
ACCCAGAACG GGCTGACAAT CACGCCGATT GTGCGACACC AGCAGCAGCA ACAACAGCAG 
CAGCAGATCG GAATACTCAA TAATGCGCAT TCCCGCAACT TGAATGGGGG ACACGCGATG 
TGCCAGCAAC AGCAGCAGCA CCCACAACTG CACCACCACT TGACAGCCGG AGCTGCCCGC 
TACAGAAAGC TAGATTCGCC CACGGATTCG GGCATTGAGT CGGGCAACGA GAAGAACGAG 
TGCAAGGCGG TGAGTTCGGG GGGAAGTTCC TCGTGCTCCA GTCCGCGTTC CAGTGTGGAT 
GATGCGCTGG ACTGCAGCGA TGCCGCCGCC AATCACAATC AGGTGGTGCA GCATCCGCAG 
CTGAGTGTGG TGTCCGTGTC ACCAGTTCGC TCGCCCCAGC CCTCCACCAG CAGCCATCTG 
AAGCGACAGA TTGTGGAGGA TATGCCCGTG CTGAAGCGCG TGCTGCAGGC TCCCCCTCTG 
TACGATACCA ACTCGCTGAT GGACGAGGCC TACAAGCCGC ACAAGAAATT CCGGGCCCTG 
CGGCATCGCG AGTTCGAGAC CGCCGAGGCG GATGCCAGCA GTTCCACTTC CGGCTCGAAC 
AGCCTGAGTG CCGGCAGTCC GCGACAGAGT CCAGTCCCGA ACAGTGTGGC CACGCCCCCG 
CCATCGGCGG CCAGCGCCGC CGCAGGTAAT CCCGCCCAGA GCCAGCTGCA CATGCACCTG 
ACCCGCAGCA GCCCCAAGGC CTCGATGGCC AGCTCGCACT CGGTGCTGGC CAAGTCTCTC 
ATGGCCGAGC CGCGCATGAC GCCCGAGCAG ATGAAGCGCA GCGATATTAT CCAAAACTAC 
TTGAAGCGCG AGAACAGCAC AGCAGCCAGC AGCACCACCA ATGGCGTGGG CAACCGCAGT 
CCCAGCAGCA GCTCCACACC GCCGCCATCG GCGGTCCAGA ATCAGCAGCG TTGGGGCAGC 
AGCTCGGTGA TCACCACCAC CTGCCAGCAG CGCCAGCAGT CCGTGTCGCC GCACAGCAAC 
GGTTCCAGCT CCAGTTCGAG CTCTAGCTCC AGCTCCAGTT CGTCATCCTC CTCCACATCC 
TCCAACTGCA GCTCCAGCTC GGCCAGCAGC TGCCAGTATT TCCAGTCGCC GCACTCCACC 
AGCAACGGCA CCAGTGCACC GGCGAGCTCC AGTTCGGGAT CGAACAGCGC CACGCCCCTG 
CTGGAACTGC AGGTGGACAT TGCTGACTCG GCGCAGCCTC TCAATTTGTC CAAGAAATCG 
CCCACGCCGC CGCCCAGCAA GCTGCACGCT CTGGTGGCCG CCGCCAATGC CGTTCAAAGG 
TATCCCACAT TGTCCGCCGA CGTCACAGTG ACAGCCTCCA ATGGCGGTCC TCCGTCGGCG 
GCGGCGAGTC CGGCGCCCAG CAGCAGTCCG CCGGCGAGTG TGGGCTCCCC CAATCCGGGC 
CTGAGCGCCG CCGTGCACAA GGTAATGCTG GAGGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eip75B ( NM_168755.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730321.1 Drosophila melanogaster Ecdysone-induced protein 75B (Eip75B), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa18848
Clone ID Related Accession (Same CDS sequence)	NM_001259893.2 , NM_168755.1 , NM_001259892.1
Accession Version	NM_168755.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ecdysone-induced protein 75B, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGCGG TCCAGGCAGC AGCGGCTGCA ACATCATCCG GAGGATCTTC AGGCAGTGTT 
CCGGGTTCCG GTTCCGGATC TGCATCGAAA CTGATCAAGA CCGAGCCCAT TGACTTTGAG 
ATGCTGCATC TGGAGGAGAA CGAACGGCAG CAGGATATCG AGCGGGAGCC GAGCAGCAGC 
AATAGCAACA GCAATAGCAA CAGTTTGACC CCCCAGCGAT ACACACACGT GCAGGTGCAG 
ACCGTGCCAC CACGCCAACC CACAGGTCTG ACCACGCCCG GTGGCACCCA GAAGGTCATT 
CTGACTCCTC GCGTAGAGTA CGTGCAACAG CGAGCCACCA GTTCCACAGG TGGTGGGATG 
AAGCACGTAT ACAGCCAACA GCAGGGCACT GCTGCATCGC GATCCGCTCC ACCCGAGACC 
ACGGCCCTAC TGACCACCAC TTCGGGCACA CCACAGATTA TCATCACTCG AACCCTACCC 
TCCAATCAGC ACCTGTCGCG CCGTCATTCG GCCAGTCCCT CCGCCCTGCA CCACTACCAG 
CAGCAGCAGC CGCAGCGTCA GCAATCGCCG CCACCGCTGC ACCACCAACA GCAGCAGCAG 
CAGCAGCATG TCCGCGTGAT CCGCGATGGC CGGCTGTACG ATGAAGCCAC GGTAGTGGTG 
GCTGCCCGTC GGCATTCGGT GTCGCCACCA CCACTGCACC ACCACTCCAG ATCGGCGCCC 
GTGTCTCCGG TGATCGCCAG GCGCGGTGGT GCTGCCGCAT ATATGGATCA GCAATATCAG 
CAACGGCAGA CGCCACCGCT GGCACCACCG CCGCCGCCTC CACCACCGCC GCCACCACCA 
CCGCCACCCC AACAGCAGCA GCAACAGTAC ATCTCCACTG GTGTTCCACC ACCCACGGCA 
GCGGCTCGGA AGTTTGTGGT TAGCACCAGC ACGCGACACG TTAATGTGAT CGCCTCCAAT 
CACTTTCAAC AACAGCAGCA GCAGCACCAA GCACAGCAGC ATCAGCAGCA GCATCAGCAG 
CATCAGCAAC ATCAGCAGCA TGTGATCGCC AGCGTTAGTT CTAGCAGCAG CTCATCGGCC 
ATTGGATCAG GCGGCTCTTC TTCTTCGCAC ATCTTTCGCA CGCCCGTGGT TTCCAGCAGC 
AGCAGCAGCA ATATGCACCA TCAGCAGCAA CAGCAACAGC AGCAATCGAG CCTGGGCAAC 
TCGGTGATGC GGCCACCTCC GCCGCCGCCT CCGCCCAAGG TCAAACACGC CTCCAGCTCC 
AGCAGCGGCA ACTCGAGCAG CAGCAACACC AACAACAGCA GCAGCAGCAG CAACGGCGAG 
GAGCCGTCTA GCTCGATTCC TGATCTAGAA TTCGATGGCA CCACAGTGCT GTGCCGCGTT 
TGCGGGGATA AGGCCTCCGG TTTCCATTAC GGCGTGCATT CCTGCGAGGG TTGCAAGGGA 
TTCTTCCGCC GCTCCATCCA GCAAAAGATC CAGTATCGCC CGTGCACCAA GAATCAGCAG 
TGCAGCATTC TGCGCATCAA TCGCAATCGT TGTCAATATT GCCGCCTGAA AAAGTGCATT 
GCCGTGGGCA TGAGTCGCGA TGCTGTGCGT TTTGGACGCG TGCCGAAGCG CGAAAAGGCG 
CGTATCCTGG CGGCCATGCA ACAGAGCACC CAGAATCGCG GCCAGCAGCG AGCCCTCGCC 
ACCGAGCTGG ATGACCAGCC ACGCCTCCTC GCCGCCGTGC TGCGCGCCCA CCTCGAGACC 
TGTGAGTTCA CCAAGGAGAA GGTCTCGGCG ATGCGGCAGC GGGCGCGGGA TTGCCCCTCC 
TACTCCATGC CCACACTTCT GGCCTGTCCG CTGAACCCCG CCCCTGAACT GCAATCGGAG 
CAGGAGTTCT CGCAGCGTTT CGCCCACGTA ATTCGCGGCG TGATCGACTT TGCCGGCATG 
ATTCCCGGCT TCCAGCTGCT CACCCAGGAC GATAAGTTCA CGCTCCTGAA GGCGGGACTC 
TTCGACGCCC TGTTTGTGCG CCTGATCTGC ATGTTTGACT CGTCGATAAA CTCAATCATC 
TGTCTAAATG GCCAGGTGAT GCGACGGGAT GCGATCCAGA ACGGAGCCAA TGCCCGCTTC 
CTGGTGGACT CCACCTTCAA TTTCGCGGAG CGCATGAACT CGATGAACCT GACAGATGCC 
GAGATAGGCC TGTTCTGCGC CATCGTTCTG ATTACGCCGG ATCGCCCCGG TTTGCGCAAC 
CTGGAGCTGA TCGAGAAGAT GTACTCGCGA CTCAAGGGCT GCCTGCAGTA CATTGTCGCC 
CAGAATAGGC CCGATCAGCC CGAGTTCCTG GCCAAGTTGC TGGAGACGAT GCCCGATCTG 
CGCACCCTGA GCACCCTGCA CACCGAGAAA CTGGTAGTTT TCCGCACCGA GCACAAGGAG 
CTGCTGCGCC AGCAGATGTG GTCCATGGAG GACGGCAACA ACAGCGATGG CCAGCAGAAC 
AAGTCGCCCT CGGGCAGCTG GGCGGATGCC ATGGACGTGG AGGCGGCCAA GAGTCCGCTT 
GGCTCGGTAT CGAGCACTGA GTCCGCCGAC CTGGACTACG GCAGTCCGAG CAGTTCGCAG 
CCACAGGGCG TGTCTCTGCC CTCGCCGCCT CAGCAACAGC CCTCGGCTCT GGCCAGCTCG 
GCTCCTCTGC TGGCGGCCAC CCTCTCCGGA GGATGTCCCC TGCGCAACCG GGCCAATTCC 
GGCTCCAGCG GTGACTCCGG AGCAGCTGAG ATGGATATCG TTGGCTCGCA CGCACATCTC 
ACCCAGAACG GGCTGACAAT CACGCCGATT GTGCGACACC AGCAGCAGCA ACAACAGCAG 
CAGCAGATCG GAATACTCAA TAATGCGCAT TCCCGCAACT TGAATGGGGG ACACGCGATG 
TGCCAGCAAC AGCAGCAGCA CCCACAACTG CACCACCACT TGACAGCCGG AGCTGCCCGC 
TACAGAAAGC TAGATTCGCC CACGGATTCG GGCATTGAGT CGGGCAACGA GAAGAACGAG 
TGCAAGGCGG TGAGTTCGGG GGGAAGTTCC TCGTGCTCCA GTCCGCGTTC CAGTGTGGAT 
GATGCGCTGG ACTGCAGCGA TGCCGCCGCC AATCACAATC AGGTGGTGCA GCATCCGCAG 
CTGAGTGTGG TGTCCGTGTC ACCAGTTCGC TCGCCCCAGC CCTCCACCAG CAGCCATCTG 
AAGCGACAGA TTGTGGAGGA TATGCCCGTG CTGAAGCGCG TGCTGCAGGC TCCCCCTCTG 
TACGATACCA ACTCGCTGAT GGACGAGGCC TACAAGCCGC ACAAGAAATT CCGGGCCCTG 
CGGCATCGCG AGTTCGAGAC CGCCGAGGCG GATGCCAGCA GTTCCACTTC CGGCTCGAAC 
AGCCTGAGTG CCGGCAGTCC GCGACAGAGT CCAGTCCCGA ACAGTGTGGC CACGCCCCCG 
CCATCGGCGG CCAGCGCCGC CGCAGGTAAT CCCGCCCAGA GCCAGCTGCA CATGCACCTG 
ACCCGCAGCA GCCCCAAGGC CTCGATGGCC AGCTCGCACT CGGTGCTGGC CAAGTCTCTC 
ATGGCCGAGC CGCGCATGAC GCCCGAGCAG ATGAAGCGCA GCGATATTAT CCAAAACTAC 
TTGAAGCGCG AGAACAGCAC AGCAGCCAGC AGCACCACCA ATGGCGTGGG CAACCGCAGT 
CCCAGCAGCA GCTCCACACC GCCGCCATCG GCGGTCCAGA ATCAGCAGCG TTGGGGCAGC 
AGCTCGGTGA TCACCACCAC CTGCCAGCAG CGCCAGCAGT CCGTGTCGCC GCACAGCAAC 
GGTTCCAGCT CCAGTTCGAG CTCTAGCTCC AGCTCCAGTT CGTCATCCTC CTCCACATCC 
TCCAACTGCA GCTCCAGCTC GGCCAGCAGC TGCCAGTATT TCCAGTCGCC GCACTCCACC 
AGCAACGGCA CCAGTGCACC GGCGAGCTCC AGTTCGGGAT CGAACAGCGC CACGCCCCTG 
CTGGAACTGC AGGTGGACAT TGCTGACTCG GCGCAGCCTC TCAATTTGTC CAAGAAATCG 
CCCACGCCGC CGCCCAGCAA GCTGCACGCT CTGGTGGCCG CCGCCAATGC CGTTCAAAGG 
TATCCCACAT TGTCCGCCGA CGTCACAGTG ACAGCCTCCA ATGGCGGTCC TCCGTCGGCG 
GCGGCGAGTC CGGCGCCCAG CAGCAGTCCG CCGGCGAGTG TGGGCTCCCC CAATCCGGGC 
CTGAGCGCCG CCGTGCACAA GGTAATGCTG GAGGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730321.1
CDS	15..4253
Translation	MEAVQAAAAATSSGGSSGSVPGSGSGSASKLIKTEPIDFEMLHLEENERQQDIEREPSSSNSNSNSNSLTPQRYTHVQVQTVPPRQPTGLTTPGGTQKVILTPRVEYVQQRATSSTGGGMKHVYSQQQGTAASRSAPPETTALLTTTSGTPQIIITRTLPSNQHLSRRHSASPSALHHYQQQQPQRQQSPPPLHHQQQQQQQHVRVIRDGRLYDEATVVVAARRHSVSPPPLHHHSRSAPVSPVIARRGGAAAYMDQQYQQRQTPPLAPPPPPPPPPPPPPPPQQQQQQYISTGVPPPTAAARKFVVSTSTRHVNVIASNHFQQQQQQHQAQQHQQQHQQHQQHQQHVIASVSSSSSSSAIGSGGSSSSHIFRTPVVSSSSSSNMHHQQQQQQQQSSLGNSVMRPPPPPPPPKVKHASSSSSGNSSSSNTNNSSSSSNGEEPSSSIPDLEFDGTTVLCRVCGDKASGFHYGVHSCEGCKGFFRRSIQQKIQYRPCTKNQQCSILRINRNRCQYCRLKKCIAVGMSRDAVRFGRVPKREKARILAAMQQSTQNRGQQRALATELDDQPRLLAAVLRAHLETCEFTKEKVSAMRQRARDCPSYSMPTLLACPLNPAPELQSEQEFSQRFAHVIRGVIDFAGMIPGFQLLTQDDKFTLLKAGLFDALFVRLICMFDSSINSIICLNGQVMRRDAIQNGANARFLVDSTFNFAERMNSMNLTDAEIGLFCAIVLITPDRPGLRNLELIEKMYSRLKGCLQYIVAQNRPDQPEFLAKLLETMPDLRTLSTLHTEKLVVFRTEHKELLRQQMWSMEDGNNSDGQQNKSPSGSWADAMDVEAAKSPLGSVSSTESADLDYGSPSSSQPQGVSLPSPPQQQPSALASSAPLLAATLSGGCPLRNRANSGSSGDSGAAEMDIVGSHAHLTQNGLTITPIVRHQQQQQQQQQIGILNNAHSRNLNGGHAMCQQQQQHPQLHHHLTAGAARYRKLDSPTDSGIESGNEKNECKAVSSGGSSSCSSPRSSVDDALDCSDAAANHNQVVQHPQLSVVSVSPVRSPQPSTSSHLKRQIVEDMPVLKRVLQAPPLYDTNSLMDEAYKPHKKFRALRHREFETAEADASSSTSGSNSLSAGSPRQSPVPNSVATPPPSAASAAAGNPAQSQLHMHLTRSSPKASMASSHSVLAKSLMAEPRMTPEQMKRSDIIQNYLKRENSTAASSTTNGVGNRSPSSSSTPPPSAVQNQQRWGSSSVITTTCQQRQQSVSPHSNGSSSSSSSSSSSSSSSSSTSSNCSSSSASSCQYFQSPHSTSNGTSAPASSSSGSNSATPLLELQVDIADSAQPLNLSKKSPTPPPSKLHALVAAANAVQRYPTLSADVTVTASNGGPPSAAASPAPSSSPPASVGSPNPGLSAAVHKVMLEA

Target ORF information:

RefSeq Version	NM_168755.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ecdysone-induced protein 75B (Eip75B), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168755.1

ORF Insert Sequence:

ATGGAGGCGG TCCAGGCAGC AGCGGCTGCA ACATCATCCG GAGGATCTTC AGGCAGTGTT 
CCGGGTTCCG GTTCCGGATC TGCATCGAAA CTGATCAAGA CCGAGCCCAT TGACTTTGAG 
ATGCTGCATC TGGAGGAGAA CGAACGGCAG CAGGATATCG AGCGGGAGCC GAGCAGCAGC 
AATAGCAACA GCAATAGCAA CAGTTTGACC CCCCAGCGAT ACACACACGT GCAGGTGCAG 
ACCGTGCCAC CACGCCAACC CACAGGTCTG ACCACGCCCG GTGGCACCCA GAAGGTCATT 
CTGACTCCTC GCGTAGAGTA CGTGCAACAG CGAGCCACCA GTTCCACAGG TGGTGGGATG 
AAGCACGTAT ACAGCCAACA GCAGGGCACT GCTGCATCGC GATCCGCTCC ACCCGAGACC 
ACGGCCCTAC TGACCACCAC TTCGGGCACA CCACAGATTA TCATCACTCG AACCCTACCC 
TCCAATCAGC ACCTGTCGCG CCGTCATTCG GCCAGTCCCT CCGCCCTGCA CCACTACCAG 
CAGCAGCAGC CGCAGCGTCA GCAATCGCCG CCACCGCTGC ACCACCAACA GCAGCAGCAG 
CAGCAGCATG TCCGCGTGAT CCGCGATGGC CGGCTGTACG ATGAAGCCAC GGTAGTGGTG 
GCTGCCCGTC GGCATTCGGT GTCGCCACCA CCACTGCACC ACCACTCCAG ATCGGCGCCC 
GTGTCTCCGG TGATCGCCAG GCGCGGTGGT GCTGCCGCAT ATATGGATCA GCAATATCAG 
CAACGGCAGA CGCCACCGCT GGCACCACCG CCGCCGCCTC CACCACCGCC GCCACCACCA 
CCGCCACCCC AACAGCAGCA GCAACAGTAC ATCTCCACTG GTGTTCCACC ACCCACGGCA 
GCGGCTCGGA AGTTTGTGGT TAGCACCAGC ACGCGACACG TTAATGTGAT CGCCTCCAAT 
CACTTTCAAC AACAGCAGCA GCAGCACCAA GCACAGCAGC ATCAGCAGCA GCATCAGCAG 
CATCAGCAAC ATCAGCAGCA TGTGATCGCC AGCGTTAGTT CTAGCAGCAG CTCATCGGCC 
ATTGGATCAG GCGGCTCTTC TTCTTCGCAC ATCTTTCGCA CGCCCGTGGT TTCCAGCAGC 
AGCAGCAGCA ATATGCACCA TCAGCAGCAA CAGCAACAGC AGCAATCGAG CCTGGGCAAC 
TCGGTGATGC GGCCACCTCC GCCGCCGCCT CCGCCCAAGG TCAAACACGC CTCCAGCTCC 
AGCAGCGGCA ACTCGAGCAG CAGCAACACC AACAACAGCA GCAGCAGCAG CAACGGCGAG 
GAGCCGTCTA GCTCGATTCC TGATCTAGAA TTCGATGGCA CCACAGTGCT GTGCCGCGTT 
TGCGGGGATA AGGCCTCCGG TTTCCATTAC GGCGTGCATT CCTGCGAGGG TTGCAAGGGA 
TTCTTCCGCC GCTCCATCCA GCAAAAGATC CAGTATCGCC CGTGCACCAA GAATCAGCAG 
TGCAGCATTC TGCGCATCAA TCGCAATCGT TGTCAATATT GCCGCCTGAA AAAGTGCATT 
GCCGTGGGCA TGAGTCGCGA TGCTGTGCGT TTTGGACGCG TGCCGAAGCG CGAAAAGGCG 
CGTATCCTGG CGGCCATGCA ACAGAGCACC CAGAATCGCG GCCAGCAGCG AGCCCTCGCC 
ACCGAGCTGG ATGACCAGCC ACGCCTCCTC GCCGCCGTGC TGCGCGCCCA CCTCGAGACC 
TGTGAGTTCA CCAAGGAGAA GGTCTCGGCG ATGCGGCAGC GGGCGCGGGA TTGCCCCTCC 
TACTCCATGC CCACACTTCT GGCCTGTCCG CTGAACCCCG CCCCTGAACT GCAATCGGAG 
CAGGAGTTCT CGCAGCGTTT CGCCCACGTA ATTCGCGGCG TGATCGACTT TGCCGGCATG 
ATTCCCGGCT TCCAGCTGCT CACCCAGGAC GATAAGTTCA CGCTCCTGAA GGCGGGACTC 
TTCGACGCCC TGTTTGTGCG CCTGATCTGC ATGTTTGACT CGTCGATAAA CTCAATCATC 
TGTCTAAATG GCCAGGTGAT GCGACGGGAT GCGATCCAGA ACGGAGCCAA TGCCCGCTTC 
CTGGTGGACT CCACCTTCAA TTTCGCGGAG CGCATGAACT CGATGAACCT GACAGATGCC 
GAGATAGGCC TGTTCTGCGC CATCGTTCTG ATTACGCCGG ATCGCCCCGG TTTGCGCAAC 
CTGGAGCTGA TCGAGAAGAT GTACTCGCGA CTCAAGGGCT GCCTGCAGTA CATTGTCGCC 
CAGAATAGGC CCGATCAGCC CGAGTTCCTG GCCAAGTTGC TGGAGACGAT GCCCGATCTG 
CGCACCCTGA GCACCCTGCA CACCGAGAAA CTGGTAGTTT TCCGCACCGA GCACAAGGAG 
CTGCTGCGCC AGCAGATGTG GTCCATGGAG GACGGCAACA ACAGCGATGG CCAGCAGAAC 
AAGTCGCCCT CGGGCAGCTG GGCGGATGCC ATGGACGTGG AGGCGGCCAA GAGTCCGCTT 
GGCTCGGTAT CGAGCACTGA GTCCGCCGAC CTGGACTACG GCAGTCCGAG CAGTTCGCAG 
CCACAGGGCG TGTCTCTGCC CTCGCCGCCT CAGCAACAGC CCTCGGCTCT GGCCAGCTCG 
GCTCCTCTGC TGGCGGCCAC CCTCTCCGGA GGATGTCCCC TGCGCAACCG GGCCAATTCC 
GGCTCCAGCG GTGACTCCGG AGCAGCTGAG ATGGATATCG TTGGCTCGCA CGCACATCTC 
ACCCAGAACG GGCTGACAAT CACGCCGATT GTGCGACACC AGCAGCAGCA ACAACAGCAG 
CAGCAGATCG GAATACTCAA TAATGCGCAT TCCCGCAACT TGAATGGGGG ACACGCGATG 
TGCCAGCAAC AGCAGCAGCA CCCACAACTG CACCACCACT TGACAGCCGG AGCTGCCCGC 
TACAGAAAGC TAGATTCGCC CACGGATTCG GGCATTGAGT CGGGCAACGA GAAGAACGAG 
TGCAAGGCGG TGAGTTCGGG GGGAAGTTCC TCGTGCTCCA GTCCGCGTTC CAGTGTGGAT 
GATGCGCTGG ACTGCAGCGA TGCCGCCGCC AATCACAATC AGGTGGTGCA GCATCCGCAG 
CTGAGTGTGG TGTCCGTGTC ACCAGTTCGC TCGCCCCAGC CCTCCACCAG CAGCCATCTG 
AAGCGACAGA TTGTGGAGGA TATGCCCGTG CTGAAGCGCG TGCTGCAGGC TCCCCCTCTG 
TACGATACCA ACTCGCTGAT GGACGAGGCC TACAAGCCGC ACAAGAAATT CCGGGCCCTG 
CGGCATCGCG AGTTCGAGAC CGCCGAGGCG GATGCCAGCA GTTCCACTTC CGGCTCGAAC 
AGCCTGAGTG CCGGCAGTCC GCGACAGAGT CCAGTCCCGA ACAGTGTGGC CACGCCCCCG 
CCATCGGCGG CCAGCGCCGC CGCAGGTAAT CCCGCCCAGA GCCAGCTGCA CATGCACCTG 
ACCCGCAGCA GCCCCAAGGC CTCGATGGCC AGCTCGCACT CGGTGCTGGC CAAGTCTCTC 
ATGGCCGAGC CGCGCATGAC GCCCGAGCAG ATGAAGCGCA GCGATATTAT CCAAAACTAC 
TTGAAGCGCG AGAACAGCAC AGCAGCCAGC AGCACCACCA ATGGCGTGGG CAACCGCAGT 
CCCAGCAGCA GCTCCACACC GCCGCCATCG GCGGTCCAGA ATCAGCAGCG TTGGGGCAGC 
AGCTCGGTGA TCACCACCAC CTGCCAGCAG CGCCAGCAGT CCGTGTCGCC GCACAGCAAC 
GGTTCCAGCT CCAGTTCGAG CTCTAGCTCC AGCTCCAGTT CGTCATCCTC CTCCACATCC 
TCCAACTGCA GCTCCAGCTC GGCCAGCAGC TGCCAGTATT TCCAGTCGCC GCACTCCACC 
AGCAACGGCA CCAGTGCACC GGCGAGCTCC AGTTCGGGAT CGAACAGCGC CACGCCCCTG 
CTGGAACTGC AGGTGGACAT TGCTGACTCG GCGCAGCCTC TCAATTTGTC CAAGAAATCG 
CCCACGCCGC CGCCCAGCAA GCTGCACGCT CTGGTGGCCG CCGCCAATGC CGTTCAAAGG 
TATCCCACAT TGTCCGCCGA CGTCACAGTG ACAGCCTCCA ATGGCGGTCC TCCGTCGGCG 
GCGGCGAGTC CGGCGCCCAG CAGCAGTCCG CCGGCGAGTG TGGGCTCCCC CAATCCGGGC 
CTGAGCGCCG CCGTGCACAA GGTAATGCTG GAGGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eip75B ( NM_001259892.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246821.1 Drosophila melanogaster Ecdysone-induced protein 75B (Eip75B), transcript variant E, mRNA.

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CloneID	OFa18848
Clone ID Related Accession (Same CDS sequence)	NM_001259893.2 , NM_168755.1 , NM_001259892.1
Accession Version	NM_001259892.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ecdysone-induced protein 75B, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGCGG TCCAGGCAGC AGCGGCTGCA ACATCATCCG GAGGATCTTC AGGCAGTGTT 
CCGGGTTCCG GTTCCGGATC TGCATCGAAA CTGATCAAGA CCGAGCCCAT TGACTTTGAG 
ATGCTGCATC TGGAGGAGAA CGAACGGCAG CAGGATATCG AGCGGGAGCC GAGCAGCAGC 
AATAGCAACA GCAATAGCAA CAGTTTGACC CCCCAGCGAT ACACACACGT GCAGGTGCAG 
ACCGTGCCAC CACGCCAACC CACAGGTCTG ACCACGCCCG GTGGCACCCA GAAGGTCATT 
CTGACTCCTC GCGTAGAGTA CGTGCAACAG CGAGCCACCA GTTCCACAGG TGGTGGGATG 
AAGCACGTAT ACAGCCAACA GCAGGGCACT GCTGCATCGC GATCCGCTCC ACCCGAGACC 
ACGGCCCTAC TGACCACCAC TTCGGGCACA CCACAGATTA TCATCACTCG AACCCTACCC 
TCCAATCAGC ACCTGTCGCG CCGTCATTCG GCCAGTCCCT CCGCCCTGCA CCACTACCAG 
CAGCAGCAGC CGCAGCGTCA GCAATCGCCG CCACCGCTGC ACCACCAACA GCAGCAGCAG 
CAGCAGCATG TCCGCGTGAT CCGCGATGGC CGGCTGTACG ATGAAGCCAC GGTAGTGGTG 
GCTGCCCGTC GGCATTCGGT GTCGCCACCA CCACTGCACC ACCACTCCAG ATCGGCGCCC 
GTGTCTCCGG TGATCGCCAG GCGCGGTGGT GCTGCCGCAT ATATGGATCA GCAATATCAG 
CAACGGCAGA CGCCACCGCT GGCACCACCG CCGCCGCCTC CACCACCGCC GCCACCACCA 
CCGCCACCCC AACAGCAGCA GCAACAGTAC ATCTCCACTG GTGTTCCACC ACCCACGGCA 
GCGGCTCGGA AGTTTGTGGT TAGCACCAGC ACGCGACACG TTAATGTGAT CGCCTCCAAT 
CACTTTCAAC AACAGCAGCA GCAGCACCAA GCACAGCAGC ATCAGCAGCA GCATCAGCAG 
CATCAGCAAC ATCAGCAGCA TGTGATCGCC AGCGTTAGTT CTAGCAGCAG CTCATCGGCC 
ATTGGATCAG GCGGCTCTTC TTCTTCGCAC ATCTTTCGCA CGCCCGTGGT TTCCAGCAGC 
AGCAGCAGCA ATATGCACCA TCAGCAGCAA CAGCAACAGC AGCAATCGAG CCTGGGCAAC 
TCGGTGATGC GGCCACCTCC GCCGCCGCCT CCGCCCAAGG TCAAACACGC CTCCAGCTCC 
AGCAGCGGCA ACTCGAGCAG CAGCAACACC AACAACAGCA GCAGCAGCAG CAACGGCGAG 
GAGCCGTCTA GCTCGATTCC TGATCTAGAA TTCGATGGCA CCACAGTGCT GTGCCGCGTT 
TGCGGGGATA AGGCCTCCGG TTTCCATTAC GGCGTGCATT CCTGCGAGGG TTGCAAGGGA 
TTCTTCCGCC GCTCCATCCA GCAAAAGATC CAGTATCGCC CGTGCACCAA GAATCAGCAG 
TGCAGCATTC TGCGCATCAA TCGCAATCGT TGTCAATATT GCCGCCTGAA AAAGTGCATT 
GCCGTGGGCA TGAGTCGCGA TGCTGTGCGT TTTGGACGCG TGCCGAAGCG CGAAAAGGCG 
CGTATCCTGG CGGCCATGCA ACAGAGCACC CAGAATCGCG GCCAGCAGCG AGCCCTCGCC 
ACCGAGCTGG ATGACCAGCC ACGCCTCCTC GCCGCCGTGC TGCGCGCCCA CCTCGAGACC 
TGTGAGTTCA CCAAGGAGAA GGTCTCGGCG ATGCGGCAGC GGGCGCGGGA TTGCCCCTCC 
TACTCCATGC CCACACTTCT GGCCTGTCCG CTGAACCCCG CCCCTGAACT GCAATCGGAG 
CAGGAGTTCT CGCAGCGTTT CGCCCACGTA ATTCGCGGCG TGATCGACTT TGCCGGCATG 
ATTCCCGGCT TCCAGCTGCT CACCCAGGAC GATAAGTTCA CGCTCCTGAA GGCGGGACTC 
TTCGACGCCC TGTTTGTGCG CCTGATCTGC ATGTTTGACT CGTCGATAAA CTCAATCATC 
TGTCTAAATG GCCAGGTGAT GCGACGGGAT GCGATCCAGA ACGGAGCCAA TGCCCGCTTC 
CTGGTGGACT CCACCTTCAA TTTCGCGGAG CGCATGAACT CGATGAACCT GACAGATGCC 
GAGATAGGCC TGTTCTGCGC CATCGTTCTG ATTACGCCGG ATCGCCCCGG TTTGCGCAAC 
CTGGAGCTGA TCGAGAAGAT GTACTCGCGA CTCAAGGGCT GCCTGCAGTA CATTGTCGCC 
CAGAATAGGC CCGATCAGCC CGAGTTCCTG GCCAAGTTGC TGGAGACGAT GCCCGATCTG 
CGCACCCTGA GCACCCTGCA CACCGAGAAA CTGGTAGTTT TCCGCACCGA GCACAAGGAG 
CTGCTGCGCC AGCAGATGTG GTCCATGGAG GACGGCAACA ACAGCGATGG CCAGCAGAAC 
AAGTCGCCCT CGGGCAGCTG GGCGGATGCC ATGGACGTGG AGGCGGCCAA GAGTCCGCTT 
GGCTCGGTAT CGAGCACTGA GTCCGCCGAC CTGGACTACG GCAGTCCGAG CAGTTCGCAG 
CCACAGGGCG TGTCTCTGCC CTCGCCGCCT CAGCAACAGC CCTCGGCTCT GGCCAGCTCG 
GCTCCTCTGC TGGCGGCCAC CCTCTCCGGA GGATGTCCCC TGCGCAACCG GGCCAATTCC 
GGCTCCAGCG GTGACTCCGG AGCAGCTGAG ATGGATATCG TTGGCTCGCA CGCACATCTC 
ACCCAGAACG GGCTGACAAT CACGCCGATT GTGCGACACC AGCAGCAGCA ACAACAGCAG 
CAGCAGATCG GAATACTCAA TAATGCGCAT TCCCGCAACT TGAATGGGGG ACACGCGATG 
TGCCAGCAAC AGCAGCAGCA CCCACAACTG CACCACCACT TGACAGCCGG AGCTGCCCGC 
TACAGAAAGC TAGATTCGCC CACGGATTCG GGCATTGAGT CGGGCAACGA GAAGAACGAG 
TGCAAGGCGG TGAGTTCGGG GGGAAGTTCC TCGTGCTCCA GTCCGCGTTC CAGTGTGGAT 
GATGCGCTGG ACTGCAGCGA TGCCGCCGCC AATCACAATC AGGTGGTGCA GCATCCGCAG 
CTGAGTGTGG TGTCCGTGTC ACCAGTTCGC TCGCCCCAGC CCTCCACCAG CAGCCATCTG 
AAGCGACAGA TTGTGGAGGA TATGCCCGTG CTGAAGCGCG TGCTGCAGGC TCCCCCTCTG 
TACGATACCA ACTCGCTGAT GGACGAGGCC TACAAGCCGC ACAAGAAATT CCGGGCCCTG 
CGGCATCGCG AGTTCGAGAC CGCCGAGGCG GATGCCAGCA GTTCCACTTC CGGCTCGAAC 
AGCCTGAGTG CCGGCAGTCC GCGACAGAGT CCAGTCCCGA ACAGTGTGGC CACGCCCCCG 
CCATCGGCGG CCAGCGCCGC CGCAGGTAAT CCCGCCCAGA GCCAGCTGCA CATGCACCTG 
ACCCGCAGCA GCCCCAAGGC CTCGATGGCC AGCTCGCACT CGGTGCTGGC CAAGTCTCTC 
ATGGCCGAGC CGCGCATGAC GCCCGAGCAG ATGAAGCGCA GCGATATTAT CCAAAACTAC 
TTGAAGCGCG AGAACAGCAC AGCAGCCAGC AGCACCACCA ATGGCGTGGG CAACCGCAGT 
CCCAGCAGCA GCTCCACACC GCCGCCATCG GCGGTCCAGA ATCAGCAGCG TTGGGGCAGC 
AGCTCGGTGA TCACCACCAC CTGCCAGCAG CGCCAGCAGT CCGTGTCGCC GCACAGCAAC 
GGTTCCAGCT CCAGTTCGAG CTCTAGCTCC AGCTCCAGTT CGTCATCCTC CTCCACATCC 
TCCAACTGCA GCTCCAGCTC GGCCAGCAGC TGCCAGTATT TCCAGTCGCC GCACTCCACC 
AGCAACGGCA CCAGTGCACC GGCGAGCTCC AGTTCGGGAT CGAACAGCGC CACGCCCCTG 
CTGGAACTGC AGGTGGACAT TGCTGACTCG GCGCAGCCTC TCAATTTGTC CAAGAAATCG 
CCCACGCCGC CGCCCAGCAA GCTGCACGCT CTGGTGGCCG CCGCCAATGC CGTTCAAAGG 
TATCCCACAT TGTCCGCCGA CGTCACAGTG ACAGCCTCCA ATGGCGGTCC TCCGTCGGCG 
GCGGCGAGTC CGGCGCCCAG CAGCAGTCCG CCGGCGAGTG TGGGCTCCCC CAATCCGGGC 
CTGAGCGCCG CCGTGCACAA GGTAATGCTG GAGGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246821.1
CDS	1328..5566
Translation	MEAVQAAAAATSSGGSSGSVPGSGSGSASKLIKTEPIDFEMLHLEENERQQDIEREPSSSNSNSNSNSLTPQRYTHVQVQTVPPRQPTGLTTPGGTQKVILTPRVEYVQQRATSSTGGGMKHVYSQQQGTAASRSAPPETTALLTTTSGTPQIIITRTLPSNQHLSRRHSASPSALHHYQQQQPQRQQSPPPLHHQQQQQQQHVRVIRDGRLYDEATVVVAARRHSVSPPPLHHHSRSAPVSPVIARRGGAAAYMDQQYQQRQTPPLAPPPPPPPPPPPPPPPQQQQQQYISTGVPPPTAAARKFVVSTSTRHVNVIASNHFQQQQQQHQAQQHQQQHQQHQQHQQHVIASVSSSSSSSAIGSGGSSSSHIFRTPVVSSSSSSNMHHQQQQQQQQSSLGNSVMRPPPPPPPPKVKHASSSSSGNSSSSNTNNSSSSSNGEEPSSSIPDLEFDGTTVLCRVCGDKASGFHYGVHSCEGCKGFFRRSIQQKIQYRPCTKNQQCSILRINRNRCQYCRLKKCIAVGMSRDAVRFGRVPKREKARILAAMQQSTQNRGQQRALATELDDQPRLLAAVLRAHLETCEFTKEKVSAMRQRARDCPSYSMPTLLACPLNPAPELQSEQEFSQRFAHVIRGVIDFAGMIPGFQLLTQDDKFTLLKAGLFDALFVRLICMFDSSINSIICLNGQVMRRDAIQNGANARFLVDSTFNFAERMNSMNLTDAEIGLFCAIVLITPDRPGLRNLELIEKMYSRLKGCLQYIVAQNRPDQPEFLAKLLETMPDLRTLSTLHTEKLVVFRTEHKELLRQQMWSMEDGNNSDGQQNKSPSGSWADAMDVEAAKSPLGSVSSTESADLDYGSPSSSQPQGVSLPSPPQQQPSALASSAPLLAATLSGGCPLRNRANSGSSGDSGAAEMDIVGSHAHLTQNGLTITPIVRHQQQQQQQQQIGILNNAHSRNLNGGHAMCQQQQQHPQLHHHLTAGAARYRKLDSPTDSGIESGNEKNECKAVSSGGSSSCSSPRSSVDDALDCSDAAANHNQVVQHPQLSVVSVSPVRSPQPSTSSHLKRQIVEDMPVLKRVLQAPPLYDTNSLMDEAYKPHKKFRALRHREFETAEADASSSTSGSNSLSAGSPRQSPVPNSVATPPPSAASAAAGNPAQSQLHMHLTRSSPKASMASSHSVLAKSLMAEPRMTPEQMKRSDIIQNYLKRENSTAASSTTNGVGNRSPSSSSTPPPSAVQNQQRWGSSSVITTTCQQRQQSVSPHSNGSSSSSSSSSSSSSSSSSTSSNCSSSSASSCQYFQSPHSTSNGTSAPASSSSGSNSATPLLELQVDIADSAQPLNLSKKSPTPPPSKLHALVAAANAVQRYPTLSADVTVTASNGGPPSAAASPAPSSSPPASVGSPNPGLSAAVHKVMLEA

Target ORF information:

RefSeq Version	NM_001259892.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ecdysone-induced protein 75B (Eip75B), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259892.1

ORF Insert Sequence:

ATGGAGGCGG TCCAGGCAGC AGCGGCTGCA ACATCATCCG GAGGATCTTC AGGCAGTGTT 
CCGGGTTCCG GTTCCGGATC TGCATCGAAA CTGATCAAGA CCGAGCCCAT TGACTTTGAG 
ATGCTGCATC TGGAGGAGAA CGAACGGCAG CAGGATATCG AGCGGGAGCC GAGCAGCAGC 
AATAGCAACA GCAATAGCAA CAGTTTGACC CCCCAGCGAT ACACACACGT GCAGGTGCAG 
ACCGTGCCAC CACGCCAACC CACAGGTCTG ACCACGCCCG GTGGCACCCA GAAGGTCATT 
CTGACTCCTC GCGTAGAGTA CGTGCAACAG CGAGCCACCA GTTCCACAGG TGGTGGGATG 
AAGCACGTAT ACAGCCAACA GCAGGGCACT GCTGCATCGC GATCCGCTCC ACCCGAGACC 
ACGGCCCTAC TGACCACCAC TTCGGGCACA CCACAGATTA TCATCACTCG AACCCTACCC 
TCCAATCAGC ACCTGTCGCG CCGTCATTCG GCCAGTCCCT CCGCCCTGCA CCACTACCAG 
CAGCAGCAGC CGCAGCGTCA GCAATCGCCG CCACCGCTGC ACCACCAACA GCAGCAGCAG 
CAGCAGCATG TCCGCGTGAT CCGCGATGGC CGGCTGTACG ATGAAGCCAC GGTAGTGGTG 
GCTGCCCGTC GGCATTCGGT GTCGCCACCA CCACTGCACC ACCACTCCAG ATCGGCGCCC 
GTGTCTCCGG TGATCGCCAG GCGCGGTGGT GCTGCCGCAT ATATGGATCA GCAATATCAG 
CAACGGCAGA CGCCACCGCT GGCACCACCG CCGCCGCCTC CACCACCGCC GCCACCACCA 
CCGCCACCCC AACAGCAGCA GCAACAGTAC ATCTCCACTG GTGTTCCACC ACCCACGGCA 
GCGGCTCGGA AGTTTGTGGT TAGCACCAGC ACGCGACACG TTAATGTGAT CGCCTCCAAT 
CACTTTCAAC AACAGCAGCA GCAGCACCAA GCACAGCAGC ATCAGCAGCA GCATCAGCAG 
CATCAGCAAC ATCAGCAGCA TGTGATCGCC AGCGTTAGTT CTAGCAGCAG CTCATCGGCC 
ATTGGATCAG GCGGCTCTTC TTCTTCGCAC ATCTTTCGCA CGCCCGTGGT TTCCAGCAGC 
AGCAGCAGCA ATATGCACCA TCAGCAGCAA CAGCAACAGC AGCAATCGAG CCTGGGCAAC 
TCGGTGATGC GGCCACCTCC GCCGCCGCCT CCGCCCAAGG TCAAACACGC CTCCAGCTCC 
AGCAGCGGCA ACTCGAGCAG CAGCAACACC AACAACAGCA GCAGCAGCAG CAACGGCGAG 
GAGCCGTCTA GCTCGATTCC TGATCTAGAA TTCGATGGCA CCACAGTGCT GTGCCGCGTT 
TGCGGGGATA AGGCCTCCGG TTTCCATTAC GGCGTGCATT CCTGCGAGGG TTGCAAGGGA 
TTCTTCCGCC GCTCCATCCA GCAAAAGATC CAGTATCGCC CGTGCACCAA GAATCAGCAG 
TGCAGCATTC TGCGCATCAA TCGCAATCGT TGTCAATATT GCCGCCTGAA AAAGTGCATT 
GCCGTGGGCA TGAGTCGCGA TGCTGTGCGT TTTGGACGCG TGCCGAAGCG CGAAAAGGCG 
CGTATCCTGG CGGCCATGCA ACAGAGCACC CAGAATCGCG GCCAGCAGCG AGCCCTCGCC 
ACCGAGCTGG ATGACCAGCC ACGCCTCCTC GCCGCCGTGC TGCGCGCCCA CCTCGAGACC 
TGTGAGTTCA CCAAGGAGAA GGTCTCGGCG ATGCGGCAGC GGGCGCGGGA TTGCCCCTCC 
TACTCCATGC CCACACTTCT GGCCTGTCCG CTGAACCCCG CCCCTGAACT GCAATCGGAG 
CAGGAGTTCT CGCAGCGTTT CGCCCACGTA ATTCGCGGCG TGATCGACTT TGCCGGCATG 
ATTCCCGGCT TCCAGCTGCT CACCCAGGAC GATAAGTTCA CGCTCCTGAA GGCGGGACTC 
TTCGACGCCC TGTTTGTGCG CCTGATCTGC ATGTTTGACT CGTCGATAAA CTCAATCATC 
TGTCTAAATG GCCAGGTGAT GCGACGGGAT GCGATCCAGA ACGGAGCCAA TGCCCGCTTC 
CTGGTGGACT CCACCTTCAA TTTCGCGGAG CGCATGAACT CGATGAACCT GACAGATGCC 
GAGATAGGCC TGTTCTGCGC CATCGTTCTG ATTACGCCGG ATCGCCCCGG TTTGCGCAAC 
CTGGAGCTGA TCGAGAAGAT GTACTCGCGA CTCAAGGGCT GCCTGCAGTA CATTGTCGCC 
CAGAATAGGC CCGATCAGCC CGAGTTCCTG GCCAAGTTGC TGGAGACGAT GCCCGATCTG 
CGCACCCTGA GCACCCTGCA CACCGAGAAA CTGGTAGTTT TCCGCACCGA GCACAAGGAG 
CTGCTGCGCC AGCAGATGTG GTCCATGGAG GACGGCAACA ACAGCGATGG CCAGCAGAAC 
AAGTCGCCCT CGGGCAGCTG GGCGGATGCC ATGGACGTGG AGGCGGCCAA GAGTCCGCTT 
GGCTCGGTAT CGAGCACTGA GTCCGCCGAC CTGGACTACG GCAGTCCGAG CAGTTCGCAG 
CCACAGGGCG TGTCTCTGCC CTCGCCGCCT CAGCAACAGC CCTCGGCTCT GGCCAGCTCG 
GCTCCTCTGC TGGCGGCCAC CCTCTCCGGA GGATGTCCCC TGCGCAACCG GGCCAATTCC 
GGCTCCAGCG GTGACTCCGG AGCAGCTGAG ATGGATATCG TTGGCTCGCA CGCACATCTC 
ACCCAGAACG GGCTGACAAT CACGCCGATT GTGCGACACC AGCAGCAGCA ACAACAGCAG 
CAGCAGATCG GAATACTCAA TAATGCGCAT TCCCGCAACT TGAATGGGGG ACACGCGATG 
TGCCAGCAAC AGCAGCAGCA CCCACAACTG CACCACCACT TGACAGCCGG AGCTGCCCGC 
TACAGAAAGC TAGATTCGCC CACGGATTCG GGCATTGAGT CGGGCAACGA GAAGAACGAG 
TGCAAGGCGG TGAGTTCGGG GGGAAGTTCC TCGTGCTCCA GTCCGCGTTC CAGTGTGGAT 
GATGCGCTGG ACTGCAGCGA TGCCGCCGCC AATCACAATC AGGTGGTGCA GCATCCGCAG 
CTGAGTGTGG TGTCCGTGTC ACCAGTTCGC TCGCCCCAGC CCTCCACCAG CAGCCATCTG 
AAGCGACAGA TTGTGGAGGA TATGCCCGTG CTGAAGCGCG TGCTGCAGGC TCCCCCTCTG 
TACGATACCA ACTCGCTGAT GGACGAGGCC TACAAGCCGC ACAAGAAATT CCGGGCCCTG 
CGGCATCGCG AGTTCGAGAC CGCCGAGGCG GATGCCAGCA GTTCCACTTC CGGCTCGAAC 
AGCCTGAGTG CCGGCAGTCC GCGACAGAGT CCAGTCCCGA ACAGTGTGGC CACGCCCCCG 
CCATCGGCGG CCAGCGCCGC CGCAGGTAAT CCCGCCCAGA GCCAGCTGCA CATGCACCTG 
ACCCGCAGCA GCCCCAAGGC CTCGATGGCC AGCTCGCACT CGGTGCTGGC CAAGTCTCTC 
ATGGCCGAGC CGCGCATGAC GCCCGAGCAG ATGAAGCGCA GCGATATTAT CCAAAACTAC 
TTGAAGCGCG AGAACAGCAC AGCAGCCAGC AGCACCACCA ATGGCGTGGG CAACCGCAGT 
CCCAGCAGCA GCTCCACACC GCCGCCATCG GCGGTCCAGA ATCAGCAGCG TTGGGGCAGC 
AGCTCGGTGA TCACCACCAC CTGCCAGCAG CGCCAGCAGT CCGTGTCGCC GCACAGCAAC 
GGTTCCAGCT CCAGTTCGAG CTCTAGCTCC AGCTCCAGTT CGTCATCCTC CTCCACATCC 
TCCAACTGCA GCTCCAGCTC GGCCAGCAGC TGCCAGTATT TCCAGTCGCC GCACTCCACC 
AGCAACGGCA CCAGTGCACC GGCGAGCTCC AGTTCGGGAT CGAACAGCGC CACGCCCCTG 
CTGGAACTGC AGGTGGACAT TGCTGACTCG GCGCAGCCTC TCAATTTGTC CAAGAAATCG 
CCCACGCCGC CGCCCAGCAA GCTGCACGCT CTGGTGGCCG CCGCCAATGC CGTTCAAAGG 
TATCCCACAT TGTCCGCCGA CGTCACAGTG ACAGCCTCCA ATGGCGGTCC TCCGTCGGCG 
GCGGCGAGTC CGGCGCCCAG CAGCAGTCCG CCGGCGAGTG TGGGCTCCCC CAATCCGGGC 
CTGAGCGCCG CCGTGCACAA GGTAATGCTG GAGGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eip75B ( NM_168756.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730322.1 Drosophila melanogaster Ecdysone-induced protein 75B (Eip75B), transcript variant C, mRNA.

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CloneID	OFa21052
Clone ID Related Accession (Same CDS sequence)	NM_168756.2
Accession Version	NM_168756.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ecdysone-induced protein 75B, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On May 8, 2012 this sequence version replaced NM_168756.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTAATGT CCGCGGACAG TTCAGATAGC GCCAAGACTT CTGTGATCTG CAGCACGGTG 
AGTGCCAGCA TGCTAGCACC ACCAGCTCCA GAACAGCCCA GCACCACAGC ACCACCCATT 
TTGGGGGTAA CAGGTCGATC TCACCTGGAA AATGCCCTGA AACTACCGCC AAACACAAGT 
GTTTCGGCTT ACTACCAGCA CAACAGCAAG CTGGGCATGG GCCAGAATTA CAATCCGGAA 
TTCAGGAGCC TGGTAGCACC TGTCACAGAT CTGGATACTG TGCCACCCAC AGGTGTGACC 
ATGGCGAGTT CTTCGAATTC TCCCAACTCC TCCGTCAAGC TGCCCCACAG CGGCGTGATC 
TTTGTCAGCA AATCGAGTGC CGTCAGCACC ACCGATGGTC CCACTGCAGT GTTGCAACAG 
CAGCAGCCGC AGCAGCAAAT GCCCCAGCAC TTCGAGTCCC TGCCCCACCA CCACCCCCAG 
CAGGAACACC AGCCACAGCA GCAGCAGCAA CAACATCACC TTCAGCACCA CCCACATCCA 
CATGTGATGT ATCCGCACGG ATATCAGCAG GCCAATCTGC ACCACTCGGG TGGTATTGCT 
GTGGTTCCGG CGGATTCGCG TCCCCAGACT CCCGAGTACA TCAAGTCCTA CCCAGTTATG 
GATACAACTG TGGCTAGTTC GGTAAAGGGG GAACCAGAAC TCAACATAGA ATTCGATGGC 
ACCACAGTGC TGTGCCGCGT TTGCGGGGAT AAGGCCTCCG GTTTCCATTA CGGCGTGCAT 
TCCTGCGAGG GTTGCAAGGG ATTCTTCCGC CGCTCCATCC AGCAAAAGAT CCAGTATCGC 
CCGTGCACCA AGAATCAGCA GTGCAGCATT CTGCGCATCA ATCGCAATCG TTGTCAATAT 
TGCCGCCTGA AAAAGTGCAT TGCCGTGGGC ATGAGTCGCG ATGCTGTGCG TTTTGGACGC 
GTGCCGAAGC GCGAAAAGGC GCGTATCCTG GCGGCCATGC AACAGAGCAC CCAGAATCGC 
GGCCAGCAGC GAGCCCTCGC CACCGAGCTG GATGACCAGC CACGCCTCCT CGCCGCCGTG 
CTGCGCGCCC ACCTCGAGAC CTGTGAGTTC ACCAAGGAGA AGGTCTCGGC GATGCGGCAG 
CGGGCGCGGG ATTGCCCCTC CTACTCCATG CCCACACTTC TGGCCTGTCC GCTGAACCCC 
GCCCCTGAAC TGCAATCGGA GCAGGAGTTC TCGCAGCGTT TCGCCCACGT AATTCGCGGC 
GTGATCGACT TTGCCGGCAT GATTCCCGGC TTCCAGCTGC TCACCCAGGA CGATAAGTTC 
ACGCTCCTGA AGGCGGGACT CTTCGACGCC CTGTTTGTGC GCCTGATCTG CATGTTTGAC 
TCGTCGATAA ACTCAATCAT CTGTCTAAAT GGCCAGGTGA TGCGACGGGA TGCGATCCAG 
AACGGAGCCA ATGCCCGCTT CCTGGTGGAC TCCACCTTCA ATTTCGCGGA GCGCATGAAC 
TCGATGAACC TGACAGATGC CGAGATAGGC CTGTTCTGCG CCATCGTTCT GATTACGCCG 
GATCGCCCCG GTTTGCGCAA CCTGGAGCTG ATCGAGAAGA TGTACTCGCG ACTCAAGGGC 
TGCCTGCAGT ACATTGTCGC CCAGAATAGG CCCGATCAGC CCGAGTTCCT GGCCAAGTTG 
CTGGAGACGA TGCCCGATCT GCGCACCCTG AGCACCCTGC ACACCGAGAA ACTGGTAGTT 
TTCCGCACCG AGCACAAGGA GCTGCTGCGC CAGCAGATGT GGTCCATGGA GGACGGCAAC 
AACAGCGATG GCCAGCAGAA CAAGTCGCCC TCGGGCAGCT GGGCGGATGC CATGGACGTG 
GAGGCGGCCA AGAGTCCGCT TGGCTCGGTA TCGAGCACTG AGTCCGCCGA CCTGGACTAC 
GGCAGTCCGA GCAGTTCGCA GCCACAGGGC GTGTCTCTGC CCTCGCCGCC TCAGCAACAG 
CCCTCGGCTC TGGCCAGCTC GGCTCCTCTG CTGGCGGCCA CCCTCTCCGG AGGATGTCCC 
CTGCGCAACC GGGCCAATTC CGGCTCCAGC GGTGACTCCG GAGCAGCTGA GATGGATATC 
GTTGGCTCGC ACGCACATCT CACCCAGAAC GGGCTGACAA TCACGCCGAT TGTGCGACAC 
CAGCAGCAGC AACAACAGCA GCAGCAGATC GGAATACTCA ATAATGCGCA TTCCCGCAAC 
TTGAATGGGG GACACGCGAT GTGCCAGCAA CAGCAGCAGC ACCCACAACT GCACCACCAC 
TTGACAGCCG GAGCTGCCCG CTACAGAAAG CTAGATTCGC CCACGGATTC GGGCATTGAG 
TCGGGCAACG AGAAGAACGA GTGCAAGGCG GTGAGTTCGG GGGGAAGTTC CTCGTGCTCC 
AGTCCGCGTT CCAGTGTGGA TGATGCGCTG GACTGCAGCG ATGCCGCCGC CAATCACAAT 
CAGGTGGTGC AGCATCCGCA GCTGAGTGTG GTGTCCGTGT CACCAGTTCG CTCGCCCCAG 
CCCTCCACCA GCAGCCATCT GAAGCGACAG ATTGTGGAGG ATATGCCCGT GCTGAAGCGC 
GTGCTGCAGG CTCCCCCTCT GTACGATACC AACTCGCTGA TGGACGAGGC CTACAAGCCG 
CACAAGAAAT TCCGGGCCCT GCGGCATCGC GAGTTCGAGA CCGCCGAGGC GGATGCCAGC 
AGTTCCACTT CCGGCTCGAA CAGCCTGAGT GCCGGCAGTC CGCGACAGAG TCCAGTCCCG 
AACAGTGTGG CCACGCCCCC GCCATCGGCG GCCAGCGCCG CCGCAGGTAA TCCCGCCCAG 
AGCCAGCTGC ACATGCACCT GACCCGCAGC AGCCCCAAGG CCTCGATGGC CAGCTCGCAC 
TCGGTGCTGG CCAAGTCTCT CATGGCCGAG CCGCGCATGA CGCCCGAGCA GATGAAGCGC 
AGCGATATTA TCCAAAACTA CTTGAAGCGC GAGAACAGCA CAGCAGCCAG CAGCACCACC 
AATGGCGTGG GCAACCGCAG TCCCAGCAGC AGCTCCACAC CGCCGCCATC GGCGGTCCAG 
AATCAGCAGC GTTGGGGCAG CAGCTCGGTG ATCACCACCA CCTGCCAGCA GCGCCAGCAG 
TCCGTGTCGC CGCACAGCAA CGGTTCCAGC TCCAGTTCGA GCTCTAGCTC CAGCTCCAGT 
TCGTCATCCT CCTCCACATC CTCCAACTGC AGCTCCAGCT CGGCCAGCAG CTGCCAGTAT 
TTCCAGTCGC CGCACTCCAC CAGCAACGGC ACCAGTGCAC CGGCGAGCTC CAGTTCGGGA 
TCGAACAGCG CCACGCCCCT GCTGGAACTG CAGGTGGACA TTGCTGACTC GGCGCAGCCT 
CTCAATTTGT CCAAGAAATC GCCCACGCCG CCGCCCAGCA AGCTGCACGC TCTGGTGGCC 
GCCGCCAATG CCGTTCAAAG GTATCCCACA TTGTCCGCCG ACGTCACAGT GACAGCCTCC 
AATGGCGGTC CTCCGTCGGC GGCGGCGAGT CCGGCGCCCA GCAGCAGTCC GCCGGCGAGT 
GTGGGCTCCC CCAATCCGGG CCTGAGCGCC GCCGTGCACA AGGTAATGCT GGAGGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730322.1
CDS	691..4290
Translation	MLMSADSSDSAKTSVICSTVSASMLAPPAPEQPSTTAPPILGVTGRSHLENALKLPPNTSVSAYYQHNSKLGMGQNYNPEFRSLVAPVTDLDTVPPTGVTMASSSNSPNSSVKLPHSGVIFVSKSSAVSTTDGPTAVLQQQQPQQQMPQHFESLPHHHPQQEHQPQQQQQQHHLQHHPHPHVMYPHGYQQANLHHSGGIAVVPADSRPQTPEYIKSYPVMDTTVASSVKGEPELNIEFDGTTVLCRVCGDKASGFHYGVHSCEGCKGFFRRSIQQKIQYRPCTKNQQCSILRINRNRCQYCRLKKCIAVGMSRDAVRFGRVPKREKARILAAMQQSTQNRGQQRALATELDDQPRLLAAVLRAHLETCEFTKEKVSAMRQRARDCPSYSMPTLLACPLNPAPELQSEQEFSQRFAHVIRGVIDFAGMIPGFQLLTQDDKFTLLKAGLFDALFVRLICMFDSSINSIICLNGQVMRRDAIQNGANARFLVDSTFNFAERMNSMNLTDAEIGLFCAIVLITPDRPGLRNLELIEKMYSRLKGCLQYIVAQNRPDQPEFLAKLLETMPDLRTLSTLHTEKLVVFRTEHKELLRQQMWSMEDGNNSDGQQNKSPSGSWADAMDVEAAKSPLGSVSSTESADLDYGSPSSSQPQGVSLPSPPQQQPSALASSAPLLAATLSGGCPLRNRANSGSSGDSGAAEMDIVGSHAHLTQNGLTITPIVRHQQQQQQQQQIGILNNAHSRNLNGGHAMCQQQQQHPQLHHHLTAGAARYRKLDSPTDSGIESGNEKNECKAVSSGGSSSCSSPRSSVDDALDCSDAAANHNQVVQHPQLSVVSVSPVRSPQPSTSSHLKRQIVEDMPVLKRVLQAPPLYDTNSLMDEAYKPHKKFRALRHREFETAEADASSSTSGSNSLSAGSPRQSPVPNSVATPPPSAASAAAGNPAQSQLHMHLTRSSPKASMASSHSVLAKSLMAEPRMTPEQMKRSDIIQNYLKRENSTAASSTTNGVGNRSPSSSSTPPPSAVQNQQRWGSSSVITTTCQQRQQSVSPHSNGSSSSSSSSSSSSSSSSSTSSNCSSSSASSCQYFQSPHSTSNGTSAPASSSSGSNSATPLLELQVDIADSAQPLNLSKKSPTPPPSKLHALVAAANAVQRYPTLSADVTVTASNGGPPSAAASPAPSSSPPASVGSPNPGLSAAVHKVMLEA

Target ORF information:

RefSeq Version	NM_168756.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ecdysone-induced protein 75B (Eip75B), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168756.2

ORF Insert Sequence:

ATGTTAATGT CCGCGGACAG TTCAGATAGC GCCAAGACTT CTGTGATCTG CAGCACGGTG 
AGTGCCAGCA TGCTAGCACC ACCAGCTCCA GAACAGCCCA GCACCACAGC ACCACCCATT 
TTGGGGGTAA CAGGTCGATC TCACCTGGAA AATGCCCTGA AACTACCGCC AAACACAAGT 
GTTTCGGCTT ACTACCAGCA CAACAGCAAG CTGGGCATGG GCCAGAATTA CAATCCGGAA 
TTCAGGAGCC TGGTAGCACC TGTCACAGAT CTGGATACTG TGCCACCCAC AGGTGTGACC 
ATGGCGAGTT CTTCGAATTC TCCCAACTCC TCCGTCAAGC TGCCCCACAG CGGCGTGATC 
TTTGTCAGCA AATCGAGTGC CGTCAGCACC ACCGATGGTC CCACTGCAGT GTTGCAACAG 
CAGCAGCCGC AGCAGCAAAT GCCCCAGCAC TTCGAGTCCC TGCCCCACCA CCACCCCCAG 
CAGGAACACC AGCCACAGCA GCAGCAGCAA CAACATCACC TTCAGCACCA CCCACATCCA 
CATGTGATGT ATCCGCACGG ATATCAGCAG GCCAATCTGC ACCACTCGGG TGGTATTGCT 
GTGGTTCCGG CGGATTCGCG TCCCCAGACT CCCGAGTACA TCAAGTCCTA CCCAGTTATG 
GATACAACTG TGGCTAGTTC GGTAAAGGGG GAACCAGAAC TCAACATAGA ATTCGATGGC 
ACCACAGTGC TGTGCCGCGT TTGCGGGGAT AAGGCCTCCG GTTTCCATTA CGGCGTGCAT 
TCCTGCGAGG GTTGCAAGGG ATTCTTCCGC CGCTCCATCC AGCAAAAGAT CCAGTATCGC 
CCGTGCACCA AGAATCAGCA GTGCAGCATT CTGCGCATCA ATCGCAATCG TTGTCAATAT 
TGCCGCCTGA AAAAGTGCAT TGCCGTGGGC ATGAGTCGCG ATGCTGTGCG TTTTGGACGC 
GTGCCGAAGC GCGAAAAGGC GCGTATCCTG GCGGCCATGC AACAGAGCAC CCAGAATCGC 
GGCCAGCAGC GAGCCCTCGC CACCGAGCTG GATGACCAGC CACGCCTCCT CGCCGCCGTG 
CTGCGCGCCC ACCTCGAGAC CTGTGAGTTC ACCAAGGAGA AGGTCTCGGC GATGCGGCAG 
CGGGCGCGGG ATTGCCCCTC CTACTCCATG CCCACACTTC TGGCCTGTCC GCTGAACCCC 
GCCCCTGAAC TGCAATCGGA GCAGGAGTTC TCGCAGCGTT TCGCCCACGT AATTCGCGGC 
GTGATCGACT TTGCCGGCAT GATTCCCGGC TTCCAGCTGC TCACCCAGGA CGATAAGTTC 
ACGCTCCTGA AGGCGGGACT CTTCGACGCC CTGTTTGTGC GCCTGATCTG CATGTTTGAC 
TCGTCGATAA ACTCAATCAT CTGTCTAAAT GGCCAGGTGA TGCGACGGGA TGCGATCCAG 
AACGGAGCCA ATGCCCGCTT CCTGGTGGAC TCCACCTTCA ATTTCGCGGA GCGCATGAAC 
TCGATGAACC TGACAGATGC CGAGATAGGC CTGTTCTGCG CCATCGTTCT GATTACGCCG 
GATCGCCCCG GTTTGCGCAA CCTGGAGCTG ATCGAGAAGA TGTACTCGCG ACTCAAGGGC 
TGCCTGCAGT ACATTGTCGC CCAGAATAGG CCCGATCAGC CCGAGTTCCT GGCCAAGTTG 
CTGGAGACGA TGCCCGATCT GCGCACCCTG AGCACCCTGC ACACCGAGAA ACTGGTAGTT 
TTCCGCACCG AGCACAAGGA GCTGCTGCGC CAGCAGATGT GGTCCATGGA GGACGGCAAC 
AACAGCGATG GCCAGCAGAA CAAGTCGCCC TCGGGCAGCT GGGCGGATGC CATGGACGTG 
GAGGCGGCCA AGAGTCCGCT TGGCTCGGTA TCGAGCACTG AGTCCGCCGA CCTGGACTAC 
GGCAGTCCGA GCAGTTCGCA GCCACAGGGC GTGTCTCTGC CCTCGCCGCC TCAGCAACAG 
CCCTCGGCTC TGGCCAGCTC GGCTCCTCTG CTGGCGGCCA CCCTCTCCGG AGGATGTCCC 
CTGCGCAACC GGGCCAATTC CGGCTCCAGC GGTGACTCCG GAGCAGCTGA GATGGATATC 
GTTGGCTCGC ACGCACATCT CACCCAGAAC GGGCTGACAA TCACGCCGAT TGTGCGACAC 
CAGCAGCAGC AACAACAGCA GCAGCAGATC GGAATACTCA ATAATGCGCA TTCCCGCAAC 
TTGAATGGGG GACACGCGAT GTGCCAGCAA CAGCAGCAGC ACCCACAACT GCACCACCAC 
TTGACAGCCG GAGCTGCCCG CTACAGAAAG CTAGATTCGC CCACGGATTC GGGCATTGAG 
TCGGGCAACG AGAAGAACGA GTGCAAGGCG GTGAGTTCGG GGGGAAGTTC CTCGTGCTCC 
AGTCCGCGTT CCAGTGTGGA TGATGCGCTG GACTGCAGCG ATGCCGCCGC CAATCACAAT 
CAGGTGGTGC AGCATCCGCA GCTGAGTGTG GTGTCCGTGT CACCAGTTCG CTCGCCCCAG 
CCCTCCACCA GCAGCCATCT GAAGCGACAG ATTGTGGAGG ATATGCCCGT GCTGAAGCGC 
GTGCTGCAGG CTCCCCCTCT GTACGATACC AACTCGCTGA TGGACGAGGC CTACAAGCCG 
CACAAGAAAT TCCGGGCCCT GCGGCATCGC GAGTTCGAGA CCGCCGAGGC GGATGCCAGC 
AGTTCCACTT CCGGCTCGAA CAGCCTGAGT GCCGGCAGTC CGCGACAGAG TCCAGTCCCG 
AACAGTGTGG CCACGCCCCC GCCATCGGCG GCCAGCGCCG CCGCAGGTAA TCCCGCCCAG 
AGCCAGCTGC ACATGCACCT GACCCGCAGC AGCCCCAAGG CCTCGATGGC CAGCTCGCAC 
TCGGTGCTGG CCAAGTCTCT CATGGCCGAG CCGCGCATGA CGCCCGAGCA GATGAAGCGC 
AGCGATATTA TCCAAAACTA CTTGAAGCGC GAGAACAGCA CAGCAGCCAG CAGCACCACC 
AATGGCGTGG GCAACCGCAG TCCCAGCAGC AGCTCCACAC CGCCGCCATC GGCGGTCCAG 
AATCAGCAGC GTTGGGGCAG CAGCTCGGTG ATCACCACCA CCTGCCAGCA GCGCCAGCAG 
TCCGTGTCGC CGCACAGCAA CGGTTCCAGC TCCAGTTCGA GCTCTAGCTC CAGCTCCAGT 
TCGTCATCCT CCTCCACATC CTCCAACTGC AGCTCCAGCT CGGCCAGCAG CTGCCAGTAT 
TTCCAGTCGC CGCACTCCAC CAGCAACGGC ACCAGTGCAC CGGCGAGCTC CAGTTCGGGA 
TCGAACAGCG CCACGCCCCT GCTGGAACTG CAGGTGGACA TTGCTGACTC GGCGCAGCCT 
CTCAATTTGT CCAAGAAATC GCCCACGCCG CCGCCCAGCA AGCTGCACGC TCTGGTGGCC 
GCCGCCAATG CCGTTCAAAG GTATCCCACA TTGTCCGCCG ACGTCACAGT GACAGCCTCC 
AATGGCGGTC CTCCGTCGGC GGCGGCGAGT CCGGCGCCCA GCAGCAGTCC GCCGGCGAGT 
GTGGGCTCCC CCAATCCGGG CCTGAGCGCC GCCGTGCACA AGGTAATGCT GGAGGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eip75B ( NM_079409.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524133.2 Drosophila melanogaster Ecdysone-induced protein 75B (Eip75B), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa21053
Clone ID Related Accession (Same CDS sequence)	NM_079409.3
Accession Version	NM_079409.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ecdysone-induced protein 75B, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On May 8, 2012 this sequence version replaced NM_079409.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTTGTG CAATGCAAGA GGTTGCTGCC GTGCAGCATC AGCAGCAGCA ACAGCAACTC 
CAGTTGCCCC AGCAGCAACA GCAGCAGCAG CAGACAACAC AGCAGCAACA TGCAACAACT 
ATAGTGCTGC TGACGGGCAA TGGCGGCGGT AATCTGCACA TTGTCGCCAC ACCGCAACAG 
CATCAGCCGA TGCATCAGCT CCACCATCAG CATCAGCATC AGCATCAGCA CCAGCAGCAG 
GCCAAGAGCC AACAGCTGAA GCAACAACAC TCGGCGCTGG TCAAGTTGCT GGAGTCGGCG 
CCCATCAAGC AGCAACAGCA GACGCCCAAG CAAATTGTTT ACCTGCAGCA GCAGCAGCAG 
CAACCGCAAC GCAAAAGACT GAAAAACGAA GCAGCAATCG TACAACAGCA ACAACAAACA 
CCTGCAACAC TAGTAAAGAC AACAACCACC AGCAACAGCA ACAGCAACAA CACCCAGACA 
ACAAATAGTA TTAGTCAGCA GCAACAGCAG CATCAGATTG TGTTGCAGCA CCAGCAGCCA 
GCCGCGGCAG CAACACCAAA GCCATGTGCC GATCTGAGCG CCAAAAATGA CAGCGAGTCG 
GGCATCGACG AGGACTCCCC CAACAGCGAT GAGGATTGCC CCAATGCCAA CCCGGCGGGC 
ACATCGCTCG AGGACAGCAG CTACGAGCAG TATCAGTGCC CCTGGAAGAA GATACGCTAT 
GCGCGTGAGC TCAAGCAGCG CGAGTTGGAG CAGCAGCAGA CCACCGGAGG CAGCAACGCG 
CAGCAGCAAG TCGAGGCGAA GCCAGCTGCA ATACCCACCA GCAACATCAA GCAGCTGCAC 
TGTGATAGTC CCTTTTCGGC GCAGACCCAC AAGGAAATCG CCAATCTCCT GCGCCAACAG 
TCCCAGCAAC AACAGGTTGT GGCCACGCAG CAGCAGCAGC AACAGCAGCA GCAGCACCAG 
CACCAGCAAC AACGAAGGGA TAGCTCCGAC AGCAACTGCT CGCTGATGAG CAACTCGAGC 
AACTCCAGTG CGGGCAATTG TTGCACCTGC AACGCTGGCG ACGACCAGCA GCTGGAGGAG 
ATGGACGAGG CCCACGATTC GGGCTGCGAC GATGAACTTT GCGAGCAGCA TCACCAGCGA 
CTGGACTCCT CCCAACTGAA TTACCTGTGC CAGAAGTTCG ATGAGAAACT GGACACGGCG 
CTGAGCAACA GCAGCGCCAA CACGGGGAGG AACACGCCAG CTGTAACAGC TAACGAAGAT 
GCCGATGGAT TCTTCCGCCG CTCCATCCAG CAAAAGATCC AGTATCGCCC GTGCACCAAG 
AATCAGCAGT GCAGCATTCT GCGCATCAAT CGCAATCGTT GTCAATATTG CCGCCTGAAA 
AAGTGCATTG CCGTGGGCAT GAGTCGCGAT GCTGTGCGTT TTGGACGCGT GCCGAAGCGC 
GAAAAGGCGC GTATCCTGGC GGCCATGCAA CAGAGCACCC AGAATCGCGG CCAGCAGCGA 
GCCCTCGCCA CCGAGCTGGA TGACCAGCCA CGCCTCCTCG CCGCCGTGCT GCGCGCCCAC 
CTCGAGACCT GTGAGTTCAC CAAGGAGAAG GTCTCGGCGA TGCGGCAGCG GGCGCGGGAT 
TGCCCCTCCT ACTCCATGCC CACACTTCTG GCCTGTCCGC TGAACCCCGC CCCTGAACTG 
CAATCGGAGC AGGAGTTCTC GCAGCGTTTC GCCCACGTAA TTCGCGGCGT GATCGACTTT 
GCCGGCATGA TTCCCGGCTT CCAGCTGCTC ACCCAGGACG ATAAGTTCAC GCTCCTGAAG 
GCGGGACTCT TCGACGCCCT GTTTGTGCGC CTGATCTGCA TGTTTGACTC GTCGATAAAC 
TCAATCATCT GTCTAAATGG CCAGGTGATG CGACGGGATG CGATCCAGAA CGGAGCCAAT 
GCCCGCTTCC TGGTGGACTC CACCTTCAAT TTCGCGGAGC GCATGAACTC GATGAACCTG 
ACAGATGCCG AGATAGGCCT GTTCTGCGCC ATCGTTCTGA TTACGCCGGA TCGCCCCGGT 
TTGCGCAACC TGGAGCTGAT CGAGAAGATG TACTCGCGAC TCAAGGGCTG CCTGCAGTAC 
ATTGTCGCCC AGAATAGGCC CGATCAGCCC GAGTTCCTGG CCAAGTTGCT GGAGACGATG 
CCCGATCTGC GCACCCTGAG CACCCTGCAC ACCGAGAAAC TGGTAGTTTT CCGCACCGAG 
CACAAGGAGC TGCTGCGCCA GCAGATGTGG TCCATGGAGG ACGGCAACAA CAGCGATGGC 
CAGCAGAACA AGTCGCCCTC GGGCAGCTGG GCGGATGCCA TGGACGTGGA GGCGGCCAAG 
AGTCCGCTTG GCTCGGTATC GAGCACTGAG TCCGCCGACC TGGACTACGG CAGTCCGAGC 
AGTTCGCAGC CACAGGGCGT GTCTCTGCCC TCGCCGCCTC AGCAACAGCC CTCGGCTCTG 
GCCAGCTCGG CTCCTCTGCT GGCGGCCACC CTCTCCGGAG GATGTCCCCT GCGCAACCGG 
GCCAATTCCG GCTCCAGCGG TGACTCCGGA GCAGCTGAGA TGGATATCGT TGGCTCGCAC 
GCACATCTCA CCCAGAACGG GCTGACAATC ACGCCGATTG TGCGACACCA GCAGCAGCAA 
CAACAGCAGC AGCAGATCGG AATACTCAAT AATGCGCATT CCCGCAACTT GAATGGGGGA 
CACGCGATGT GCCAGCAACA GCAGCAGCAC CCACAACTGC ACCACCACTT GACAGCCGGA 
GCTGCCCGCT ACAGAAAGCT AGATTCGCCC ACGGATTCGG GCATTGAGTC GGGCAACGAG 
AAGAACGAGT GCAAGGCGGT GAGTTCGGGG GGAAGTTCCT CGTGCTCCAG TCCGCGTTCC 
AGTGTGGATG ATGCGCTGGA CTGCAGCGAT GCCGCCGCCA ATCACAATCA GGTGGTGCAG 
CATCCGCAGC TGAGTGTGGT GTCCGTGTCA CCAGTTCGCT CGCCCCAGCC CTCCACCAGC 
AGCCATCTGA AGCGACAGAT TGTGGAGGAT ATGCCCGTGC TGAAGCGCGT GCTGCAGGCT 
CCCCCTCTGT ACGATACCAA CTCGCTGATG GACGAGGCCT ACAAGCCGCA CAAGAAATTC 
CGGGCCCTGC GGCATCGCGA GTTCGAGACC GCCGAGGCGG ATGCCAGCAG TTCCACTTCC 
GGCTCGAACA GCCTGAGTGC CGGCAGTCCG CGACAGAGTC CAGTCCCGAA CAGTGTGGCC 
ACGCCCCCGC CATCGGCGGC CAGCGCCGCC GCAGGTAATC CCGCCCAGAG CCAGCTGCAC 
ATGCACCTGA CCCGCAGCAG CCCCAAGGCC TCGATGGCCA GCTCGCACTC GGTGCTGGCC 
AAGTCTCTCA TGGCCGAGCC GCGCATGACG CCCGAGCAGA TGAAGCGCAG CGATATTATC 
CAAAACTACT TGAAGCGCGA GAACAGCACA GCAGCCAGCA GCACCACCAA TGGCGTGGGC 
AACCGCAGTC CCAGCAGCAG CTCCACACCG CCGCCATCGG CGGTCCAGAA TCAGCAGCGT 
TGGGGCAGCA GCTCGGTGAT CACCACCACC TGCCAGCAGC GCCAGCAGTC CGTGTCGCCG 
CACAGCAACG GTTCCAGCTC CAGTTCGAGC TCTAGCTCCA GCTCCAGTTC GTCATCCTCC 
TCCACATCCT CCAACTGCAG CTCCAGCTCG GCCAGCAGCT GCCAGTATTT CCAGTCGCCG 
CACTCCACCA GCAACGGCAC CAGTGCACCG GCGAGCTCCA GTTCGGGATC GAACAGCGCC 
ACGCCCCTGC TGGAACTGCA GGTGGACATT GCTGACTCGG CGCAGCCTCT CAATTTGTCC 
AAGAAATCGC CCACGCCGCC GCCCAGCAAG CTGCACGCTC TGGTGGCCGC CGCCAATGCC 
GTTCAAAGGT ATCCCACATT GTCCGCCGAC GTCACAGTGA CAGCCTCCAA TGGCGGTCCT 
CCGTCGGCGG CGGCGAGTCC GGCGCCCAGC AGCAGTCCGC CGGCGAGTGT GGGCTCCCCC 
AATCCGGGCC TGAGCGCCGC CGTGCACAAG GTAATGCTGG AGGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524133.2
CDS	531..4598
Translation	MVCAMQEVAAVQHQQQQQQLQLPQQQQQQQQTTQQQHATTIVLLTGNGGGNLHIVATPQQHQPMHQLHHQHQHQHQHQQQAKSQQLKQQHSALVKLLESAPIKQQQQTPKQIVYLQQQQQQPQRKRLKNEAAIVQQQQQTPATLVKTTTTSNSNSNNTQTTNSISQQQQQHQIVLQHQQPAAAATPKPCADLSAKNDSESGIDEDSPNSDEDCPNANPAGTSLEDSSYEQYQCPWKKIRYARELKQRELEQQQTTGGSNAQQQVEAKPAAIPTSNIKQLHCDSPFSAQTHKEIANLLRQQSQQQQVVATQQQQQQQQQHQHQQQRRDSSDSNCSLMSNSSNSSAGNCCTCNAGDDQQLEEMDEAHDSGCDDELCEQHHQRLDSSQLNYLCQKFDEKLDTALSNSSANTGRNTPAVTANEDADGFFRRSIQQKIQYRPCTKNQQCSILRINRNRCQYCRLKKCIAVGMSRDAVRFGRVPKREKARILAAMQQSTQNRGQQRALATELDDQPRLLAAVLRAHLETCEFTKEKVSAMRQRARDCPSYSMPTLLACPLNPAPELQSEQEFSQRFAHVIRGVIDFAGMIPGFQLLTQDDKFTLLKAGLFDALFVRLICMFDSSINSIICLNGQVMRRDAIQNGANARFLVDSTFNFAERMNSMNLTDAEIGLFCAIVLITPDRPGLRNLELIEKMYSRLKGCLQYIVAQNRPDQPEFLAKLLETMPDLRTLSTLHTEKLVVFRTEHKELLRQQMWSMEDGNNSDGQQNKSPSGSWADAMDVEAAKSPLGSVSSTESADLDYGSPSSSQPQGVSLPSPPQQQPSALASSAPLLAATLSGGCPLRNRANSGSSGDSGAAEMDIVGSHAHLTQNGLTITPIVRHQQQQQQQQQIGILNNAHSRNLNGGHAMCQQQQQHPQLHHHLTAGAARYRKLDSPTDSGIESGNEKNECKAVSSGGSSSCSSPRSSVDDALDCSDAAANHNQVVQHPQLSVVSVSPVRSPQPSTSSHLKRQIVEDMPVLKRVLQAPPLYDTNSLMDEAYKPHKKFRALRHREFETAEADASSSTSGSNSLSAGSPRQSPVPNSVATPPPSAASAAAGNPAQSQLHMHLTRSSPKASMASSHSVLAKSLMAEPRMTPEQMKRSDIIQNYLKRENSTAASSTTNGVGNRSPSSSSTPPPSAVQNQQRWGSSSVITTTCQQRQQSVSPHSNGSSSSSSSSSSSSSSSSSTSSNCSSSSASSCQYFQSPHSTSNGTSAPASSSSGSNSATPLLELQVDIADSAQPLNLSKKSPTPPPSKLHALVAAANAVQRYPTLSADVTVTASNGGPPSAAASPAPSSSPPASVGSPNPGLSAAVHKVMLEA

Target ORF information:

RefSeq Version	NM_079409.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ecdysone-induced protein 75B (Eip75B), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079409.3

ORF Insert Sequence:

ATGGTTTGTG CAATGCAAGA GGTTGCTGCC GTGCAGCATC AGCAGCAGCA ACAGCAACTC 
CAGTTGCCCC AGCAGCAACA GCAGCAGCAG CAGACAACAC AGCAGCAACA TGCAACAACT 
ATAGTGCTGC TGACGGGCAA TGGCGGCGGT AATCTGCACA TTGTCGCCAC ACCGCAACAG 
CATCAGCCGA TGCATCAGCT CCACCATCAG CATCAGCATC AGCATCAGCA CCAGCAGCAG 
GCCAAGAGCC AACAGCTGAA GCAACAACAC TCGGCGCTGG TCAAGTTGCT GGAGTCGGCG 
CCCATCAAGC AGCAACAGCA GACGCCCAAG CAAATTGTTT ACCTGCAGCA GCAGCAGCAG 
CAACCGCAAC GCAAAAGACT GAAAAACGAA GCAGCAATCG TACAACAGCA ACAACAAACA 
CCTGCAACAC TAGTAAAGAC AACAACCACC AGCAACAGCA ACAGCAACAA CACCCAGACA 
ACAAATAGTA TTAGTCAGCA GCAACAGCAG CATCAGATTG TGTTGCAGCA CCAGCAGCCA 
GCCGCGGCAG CAACACCAAA GCCATGTGCC GATCTGAGCG CCAAAAATGA CAGCGAGTCG 
GGCATCGACG AGGACTCCCC CAACAGCGAT GAGGATTGCC CCAATGCCAA CCCGGCGGGC 
ACATCGCTCG AGGACAGCAG CTACGAGCAG TATCAGTGCC CCTGGAAGAA GATACGCTAT 
GCGCGTGAGC TCAAGCAGCG CGAGTTGGAG CAGCAGCAGA CCACCGGAGG CAGCAACGCG 
CAGCAGCAAG TCGAGGCGAA GCCAGCTGCA ATACCCACCA GCAACATCAA GCAGCTGCAC 
TGTGATAGTC CCTTTTCGGC GCAGACCCAC AAGGAAATCG CCAATCTCCT GCGCCAACAG 
TCCCAGCAAC AACAGGTTGT GGCCACGCAG CAGCAGCAGC AACAGCAGCA GCAGCACCAG 
CACCAGCAAC AACGAAGGGA TAGCTCCGAC AGCAACTGCT CGCTGATGAG CAACTCGAGC 
AACTCCAGTG CGGGCAATTG TTGCACCTGC AACGCTGGCG ACGACCAGCA GCTGGAGGAG 
ATGGACGAGG CCCACGATTC GGGCTGCGAC GATGAACTTT GCGAGCAGCA TCACCAGCGA 
CTGGACTCCT CCCAACTGAA TTACCTGTGC CAGAAGTTCG ATGAGAAACT GGACACGGCG 
CTGAGCAACA GCAGCGCCAA CACGGGGAGG AACACGCCAG CTGTAACAGC TAACGAAGAT 
GCCGATGGAT TCTTCCGCCG CTCCATCCAG CAAAAGATCC AGTATCGCCC GTGCACCAAG 
AATCAGCAGT GCAGCATTCT GCGCATCAAT CGCAATCGTT GTCAATATTG CCGCCTGAAA 
AAGTGCATTG CCGTGGGCAT GAGTCGCGAT GCTGTGCGTT TTGGACGCGT GCCGAAGCGC 
GAAAAGGCGC GTATCCTGGC GGCCATGCAA CAGAGCACCC AGAATCGCGG CCAGCAGCGA 
GCCCTCGCCA CCGAGCTGGA TGACCAGCCA CGCCTCCTCG CCGCCGTGCT GCGCGCCCAC 
CTCGAGACCT GTGAGTTCAC CAAGGAGAAG GTCTCGGCGA TGCGGCAGCG GGCGCGGGAT 
TGCCCCTCCT ACTCCATGCC CACACTTCTG GCCTGTCCGC TGAACCCCGC CCCTGAACTG 
CAATCGGAGC AGGAGTTCTC GCAGCGTTTC GCCCACGTAA TTCGCGGCGT GATCGACTTT 
GCCGGCATGA TTCCCGGCTT CCAGCTGCTC ACCCAGGACG ATAAGTTCAC GCTCCTGAAG 
GCGGGACTCT TCGACGCCCT GTTTGTGCGC CTGATCTGCA TGTTTGACTC GTCGATAAAC 
TCAATCATCT GTCTAAATGG CCAGGTGATG CGACGGGATG CGATCCAGAA CGGAGCCAAT 
GCCCGCTTCC TGGTGGACTC CACCTTCAAT TTCGCGGAGC GCATGAACTC GATGAACCTG 
ACAGATGCCG AGATAGGCCT GTTCTGCGCC ATCGTTCTGA TTACGCCGGA TCGCCCCGGT 
TTGCGCAACC TGGAGCTGAT CGAGAAGATG TACTCGCGAC TCAAGGGCTG CCTGCAGTAC 
ATTGTCGCCC AGAATAGGCC CGATCAGCCC GAGTTCCTGG CCAAGTTGCT GGAGACGATG 
CCCGATCTGC GCACCCTGAG CACCCTGCAC ACCGAGAAAC TGGTAGTTTT CCGCACCGAG 
CACAAGGAGC TGCTGCGCCA GCAGATGTGG TCCATGGAGG ACGGCAACAA CAGCGATGGC 
CAGCAGAACA AGTCGCCCTC GGGCAGCTGG GCGGATGCCA TGGACGTGGA GGCGGCCAAG 
AGTCCGCTTG GCTCGGTATC GAGCACTGAG TCCGCCGACC TGGACTACGG CAGTCCGAGC 
AGTTCGCAGC CACAGGGCGT GTCTCTGCCC TCGCCGCCTC AGCAACAGCC CTCGGCTCTG 
GCCAGCTCGG CTCCTCTGCT GGCGGCCACC CTCTCCGGAG GATGTCCCCT GCGCAACCGG 
GCCAATTCCG GCTCCAGCGG TGACTCCGGA GCAGCTGAGA TGGATATCGT TGGCTCGCAC 
GCACATCTCA CCCAGAACGG GCTGACAATC ACGCCGATTG TGCGACACCA GCAGCAGCAA 
CAACAGCAGC AGCAGATCGG AATACTCAAT AATGCGCATT CCCGCAACTT GAATGGGGGA 
CACGCGATGT GCCAGCAACA GCAGCAGCAC CCACAACTGC ACCACCACTT GACAGCCGGA 
GCTGCCCGCT ACAGAAAGCT AGATTCGCCC ACGGATTCGG GCATTGAGTC GGGCAACGAG 
AAGAACGAGT GCAAGGCGGT GAGTTCGGGG GGAAGTTCCT CGTGCTCCAG TCCGCGTTCC 
AGTGTGGATG ATGCGCTGGA CTGCAGCGAT GCCGCCGCCA ATCACAATCA GGTGGTGCAG 
CATCCGCAGC TGAGTGTGGT GTCCGTGTCA CCAGTTCGCT CGCCCCAGCC CTCCACCAGC 
AGCCATCTGA AGCGACAGAT TGTGGAGGAT ATGCCCGTGC TGAAGCGCGT GCTGCAGGCT 
CCCCCTCTGT ACGATACCAA CTCGCTGATG GACGAGGCCT ACAAGCCGCA CAAGAAATTC 
CGGGCCCTGC GGCATCGCGA GTTCGAGACC GCCGAGGCGG ATGCCAGCAG TTCCACTTCC 
GGCTCGAACA GCCTGAGTGC CGGCAGTCCG CGACAGAGTC CAGTCCCGAA CAGTGTGGCC 
ACGCCCCCGC CATCGGCGGC CAGCGCCGCC GCAGGTAATC CCGCCCAGAG CCAGCTGCAC 
ATGCACCTGA CCCGCAGCAG CCCCAAGGCC TCGATGGCCA GCTCGCACTC GGTGCTGGCC 
AAGTCTCTCA TGGCCGAGCC GCGCATGACG CCCGAGCAGA TGAAGCGCAG CGATATTATC 
CAAAACTACT TGAAGCGCGA GAACAGCACA GCAGCCAGCA GCACCACCAA TGGCGTGGGC 
AACCGCAGTC CCAGCAGCAG CTCCACACCG CCGCCATCGG CGGTCCAGAA TCAGCAGCGT 
TGGGGCAGCA GCTCGGTGAT CACCACCACC TGCCAGCAGC GCCAGCAGTC CGTGTCGCCG 
CACAGCAACG GTTCCAGCTC CAGTTCGAGC TCTAGCTCCA GCTCCAGTTC GTCATCCTCC 
TCCACATCCT CCAACTGCAG CTCCAGCTCG GCCAGCAGCT GCCAGTATTT CCAGTCGCCG 
CACTCCACCA GCAACGGCAC CAGTGCACCG GCGAGCTCCA GTTCGGGATC GAACAGCGCC 
ACGCCCCTGC TGGAACTGCA GGTGGACATT GCTGACTCGG CGCAGCCTCT CAATTTGTCC 
AAGAAATCGC CCACGCCGCC GCCCAGCAAG CTGCACGCTC TGGTGGCCGC CGCCAATGCC 
GTTCAAAGGT ATCCCACATT GTCCGCCGAC GTCACAGTGA CAGCCTCCAA TGGCGGTCCT 
CCGTCGGCGG CGGCGAGTCC GGCGCCCAGC AGCAGTCCGC CGGCGAGTGT GGGCTCCCCC 
AATCCGGGCC TGAGCGCCGC CGTGCACAAG GTAATGCTGG AGGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eip75B ( NM_168757.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730323.1 Drosophila melanogaster Ecdysone-induced protein 75B (Eip75B), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa21054
Clone ID Related Accession (Same CDS sequence)	NM_168757.1
Accession Version	NM_168757.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ecdysone-induced protein 75B, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGAAG AACTCCCGAT ATTGAAGGGC ATACTTAAAG GCAACGTCAA CTATCACAAT 
GCGCCTGTGC GTTTTGGACG CGTGCCGAAG CGCGAAAAGG CGCGTATCCT GGCGGCCATG 
CAACAGAGCA CCCAGAATCG CGGCCAGCAG CGAGCCCTCG CCACCGAGCT GGATGACCAG 
CCACGCCTCC TCGCCGCCGT GCTGCGCGCC CACCTCGAGA CCTGTGAGTT CACCAAGGAG 
AAGGTCTCGG CGATGCGGCA GCGGGCGCGG GATTGCCCCT CCTACTCCAT GCCCACACTT 
CTGGCCTGTC CGCTGAACCC CGCCCCTGAA CTGCAATCGG AGCAGGAGTT CTCGCAGCGT 
TTCGCCCACG TAATTCGCGG CGTGATCGAC TTTGCCGGCA TGATTCCCGG CTTCCAGCTG 
CTCACCCAGG ACGATAAGTT CACGCTCCTG AAGGCGGGAC TCTTCGACGC CCTGTTTGTG 
CGCCTGATCT GCATGTTTGA CTCGTCGATA AACTCAATCA TCTGTCTAAA TGGCCAGGTG 
ATGCGACGGG ATGCGATCCA GAACGGAGCC AATGCCCGCT TCCTGGTGGA CTCCACCTTC 
AATTTCGCGG AGCGCATGAA CTCGATGAAC CTGACAGATG CCGAGATAGG CCTGTTCTGC 
GCCATCGTTC TGATTACGCC GGATCGCCCC GGTTTGCGCA ACCTGGAGCT GATCGAGAAG 
ATGTACTCGC GACTCAAGGG CTGCCTGCAG TACATTGTCG CCCAGAATAG GCCCGATCAG 
CCCGAGTTCC TGGCCAAGTT GCTGGAGACG ATGCCCGATC TGCGCACCCT GAGCACCCTG 
CACACCGAGA AACTGGTAGT TTTCCGCACC GAGCACAAGG AGCTGCTGCG CCAGCAGATG 
TGGTCCATGG AGGACGGCAA CAACAGCGAT GGCCAGCAGA ACAAGTCGCC CTCGGGCAGC 
TGGGCGGATG CCATGGACGT GGAGGCGGCC AAGAGTCCGC TTGGCTCGGT ATCGAGCACT 
GAGTCCGCCG ACCTGGACTA CGGCAGTCCG AGCAGTTCGC AGCCACAGGG CGTGTCTCTG 
CCCTCGCCGC CTCAGCAACA GCCCTCGGCT CTGGCCAGCT CGGCTCCTCT GCTGGCGGCC 
ACCCTCTCCG GAGGATGTCC CCTGCGCAAC CGGGCCAATT CCGGCTCCAG CGGTGACTCC 
GGAGCAGCTG AGATGGATAT CGTTGGCTCG CACGCACATC TCACCCAGAA CGGGCTGACA 
ATCACGCCGA TTGTGCGACA CCAGCAGCAG CAACAACAGC AGCAGCAGAT CGGAATACTC 
AATAATGCGC ATTCCCGCAA CTTGAATGGG GGACACGCGA TGTGCCAGCA ACAGCAGCAG 
CACCCACAAC TGCACCACCA CTTGACAGCC GGAGCTGCCC GCTACAGAAA GCTAGATTCG 
CCCACGGATT CGGGCATTGA GTCGGGCAAC GAGAAGAACG AGTGCAAGGC GGTGAGTTCG 
GGGGGAAGTT CCTCGTGCTC CAGTCCGCGT TCCAGTGTGG ATGATGCGCT GGACTGCAGC 
GATGCCGCCG CCAATCACAA TCAGGTGGTG CAGCATCCGC AGCTGAGTGT GGTGTCCGTG 
TCACCAGTTC GCTCGCCCCA GCCCTCCACC AGCAGCCATC TGAAGCGACA GATTGTGGAG 
GATATGCCCG TGCTGAAGCG CGTGCTGCAG GCTCCCCCTC TGTACGATAC CAACTCGCTG 
ATGGACGAGG CCTACAAGCC GCACAAGAAA TTCCGGGCCC TGCGGCATCG CGAGTTCGAG 
ACCGCCGAGG CGGATGCCAG CAGTTCCACT TCCGGCTCGA ACAGCCTGAG TGCCGGCAGT 
CCGCGACAGA GTCCAGTCCC GAACAGTGTG GCCACGCCCC CGCCATCGGC GGCCAGCGCC 
GCCGCAGGTA ATCCCGCCCA GAGCCAGCTG CACATGCACC TGACCCGCAG CAGCCCCAAG 
GCCTCGATGG CCAGCTCGCA CTCGGTGCTG GCCAAGTCTC TCATGGCCGA GCCGCGCATG 
ACGCCCGAGC AGATGAAGCG CAGCGATATT ATCCAAAACT ACTTGAAGCG CGAGAACAGC 
ACAGCAGCCA GCAGCACCAC CAATGGCGTG GGCAACCGCA GTCCCAGCAG CAGCTCCACA 
CCGCCGCCAT CGGCGGTCCA GAATCAGCAG CGTTGGGGCA GCAGCTCGGT GATCACCACC 
ACCTGCCAGC AGCGCCAGCA GTCCGTGTCG CCGCACAGCA ACGGTTCCAG CTCCAGTTCG 
AGCTCTAGCT CCAGCTCCAG TTCGTCATCC TCCTCCACAT CCTCCAACTG CAGCTCCAGC 
TCGGCCAGCA GCTGCCAGTA TTTCCAGTCG CCGCACTCCA CCAGCAACGG CACCAGTGCA 
CCGGCGAGCT CCAGTTCGGG ATCGAACAGC GCCACGCCCC TGCTGGAACT GCAGGTGGAC 
ATTGCTGACT CGGCGCAGCC TCTCAATTTG TCCAAGAAAT CGCCCACGCC GCCGCCCAGC 
AAGCTGCACG CTCTGGTGGC CGCCGCCAAT GCCGTTCAAA GGTATCCCAC ATTGTCCGCC 
GACGTCACAG TGACAGCCTC CAATGGCGGT CCTCCGTCGG CGGCGGCGAG TCCGGCGCCC 
AGCAGCAGTC CGCCGGCGAG TGTGGGCTCC CCCAATCCGG GCCTGAGCGC CGCCGTGCAC 
AAGGTAATGC TGGAGGCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730323.1
CDS	747..3467
Translation	MGEELPILKGILKGNVNYHNAPVRFGRVPKREKARILAAMQQSTQNRGQQRALATELDDQPRLLAAVLRAHLETCEFTKEKVSAMRQRARDCPSYSMPTLLACPLNPAPELQSEQEFSQRFAHVIRGVIDFAGMIPGFQLLTQDDKFTLLKAGLFDALFVRLICMFDSSINSIICLNGQVMRRDAIQNGANARFLVDSTFNFAERMNSMNLTDAEIGLFCAIVLITPDRPGLRNLELIEKMYSRLKGCLQYIVAQNRPDQPEFLAKLLETMPDLRTLSTLHTEKLVVFRTEHKELLRQQMWSMEDGNNSDGQQNKSPSGSWADAMDVEAAKSPLGSVSSTESADLDYGSPSSSQPQGVSLPSPPQQQPSALASSAPLLAATLSGGCPLRNRANSGSSGDSGAAEMDIVGSHAHLTQNGLTITPIVRHQQQQQQQQQIGILNNAHSRNLNGGHAMCQQQQQHPQLHHHLTAGAARYRKLDSPTDSGIESGNEKNECKAVSSGGSSSCSSPRSSVDDALDCSDAAANHNQVVQHPQLSVVSVSPVRSPQPSTSSHLKRQIVEDMPVLKRVLQAPPLYDTNSLMDEAYKPHKKFRALRHREFETAEADASSSTSGSNSLSAGSPRQSPVPNSVATPPPSAASAAAGNPAQSQLHMHLTRSSPKASMASSHSVLAKSLMAEPRMTPEQMKRSDIIQNYLKRENSTAASSTTNGVGNRSPSSSSTPPPSAVQNQQRWGSSSVITTTCQQRQQSVSPHSNGSSSSSSSSSSSSSSSSSTSSNCSSSSASSCQYFQSPHSTSNGTSAPASSSSGSNSATPLLELQVDIADSAQPLNLSKKSPTPPPSKLHALVAAANAVQRYPTLSADVTVTASNGGPPSAAASPAPSSSPPASVGSPNPGLSAAVHKVMLEA

Target ORF information:

RefSeq Version	NM_168757.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ecdysone-induced protein 75B (Eip75B), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168757.1

ORF Insert Sequence:

ATGGGCGAAG AACTCCCGAT ATTGAAGGGC ATACTTAAAG GCAACGTCAA CTATCACAAT 
GCGCCTGTGC GTTTTGGACG CGTGCCGAAG CGCGAAAAGG CGCGTATCCT GGCGGCCATG 
CAACAGAGCA CCCAGAATCG CGGCCAGCAG CGAGCCCTCG CCACCGAGCT GGATGACCAG 
CCACGCCTCC TCGCCGCCGT GCTGCGCGCC CACCTCGAGA CCTGTGAGTT CACCAAGGAG 
AAGGTCTCGG CGATGCGGCA GCGGGCGCGG GATTGCCCCT CCTACTCCAT GCCCACACTT 
CTGGCCTGTC CGCTGAACCC CGCCCCTGAA CTGCAATCGG AGCAGGAGTT CTCGCAGCGT 
TTCGCCCACG TAATTCGCGG CGTGATCGAC TTTGCCGGCA TGATTCCCGG CTTCCAGCTG 
CTCACCCAGG ACGATAAGTT CACGCTCCTG AAGGCGGGAC TCTTCGACGC CCTGTTTGTG 
CGCCTGATCT GCATGTTTGA CTCGTCGATA AACTCAATCA TCTGTCTAAA TGGCCAGGTG 
ATGCGACGGG ATGCGATCCA GAACGGAGCC AATGCCCGCT TCCTGGTGGA CTCCACCTTC 
AATTTCGCGG AGCGCATGAA CTCGATGAAC CTGACAGATG CCGAGATAGG CCTGTTCTGC 
GCCATCGTTC TGATTACGCC GGATCGCCCC GGTTTGCGCA ACCTGGAGCT GATCGAGAAG 
ATGTACTCGC GACTCAAGGG CTGCCTGCAG TACATTGTCG CCCAGAATAG GCCCGATCAG 
CCCGAGTTCC TGGCCAAGTT GCTGGAGACG ATGCCCGATC TGCGCACCCT GAGCACCCTG 
CACACCGAGA AACTGGTAGT TTTCCGCACC GAGCACAAGG AGCTGCTGCG CCAGCAGATG 
TGGTCCATGG AGGACGGCAA CAACAGCGAT GGCCAGCAGA ACAAGTCGCC CTCGGGCAGC 
TGGGCGGATG CCATGGACGT GGAGGCGGCC AAGAGTCCGC TTGGCTCGGT ATCGAGCACT 
GAGTCCGCCG ACCTGGACTA CGGCAGTCCG AGCAGTTCGC AGCCACAGGG CGTGTCTCTG 
CCCTCGCCGC CTCAGCAACA GCCCTCGGCT CTGGCCAGCT CGGCTCCTCT GCTGGCGGCC 
ACCCTCTCCG GAGGATGTCC CCTGCGCAAC CGGGCCAATT CCGGCTCCAG CGGTGACTCC 
GGAGCAGCTG AGATGGATAT CGTTGGCTCG CACGCACATC TCACCCAGAA CGGGCTGACA 
ATCACGCCGA TTGTGCGACA CCAGCAGCAG CAACAACAGC AGCAGCAGAT CGGAATACTC 
AATAATGCGC ATTCCCGCAA CTTGAATGGG GGACACGCGA TGTGCCAGCA ACAGCAGCAG 
CACCCACAAC TGCACCACCA CTTGACAGCC GGAGCTGCCC GCTACAGAAA GCTAGATTCG 
CCCACGGATT CGGGCATTGA GTCGGGCAAC GAGAAGAACG AGTGCAAGGC GGTGAGTTCG 
GGGGGAAGTT CCTCGTGCTC CAGTCCGCGT TCCAGTGTGG ATGATGCGCT GGACTGCAGC 
GATGCCGCCG CCAATCACAA TCAGGTGGTG CAGCATCCGC AGCTGAGTGT GGTGTCCGTG 
TCACCAGTTC GCTCGCCCCA GCCCTCCACC AGCAGCCATC TGAAGCGACA GATTGTGGAG 
GATATGCCCG TGCTGAAGCG CGTGCTGCAG GCTCCCCCTC TGTACGATAC CAACTCGCTG 
ATGGACGAGG CCTACAAGCC GCACAAGAAA TTCCGGGCCC TGCGGCATCG CGAGTTCGAG 
ACCGCCGAGG CGGATGCCAG CAGTTCCACT TCCGGCTCGA ACAGCCTGAG TGCCGGCAGT 
CCGCGACAGA GTCCAGTCCC GAACAGTGTG GCCACGCCCC CGCCATCGGC GGCCAGCGCC 
GCCGCAGGTA ATCCCGCCCA GAGCCAGCTG CACATGCACC TGACCCGCAG CAGCCCCAAG 
GCCTCGATGG CCAGCTCGCA CTCGGTGCTG GCCAAGTCTC TCATGGCCGA GCCGCGCATG 
ACGCCCGAGC AGATGAAGCG CAGCGATATT ATCCAAAACT ACTTGAAGCG CGAGAACAGC 
ACAGCAGCCA GCAGCACCAC CAATGGCGTG GGCAACCGCA GTCCCAGCAG CAGCTCCACA 
CCGCCGCCAT CGGCGGTCCA GAATCAGCAG CGTTGGGGCA GCAGCTCGGT GATCACCACC 
ACCTGCCAGC AGCGCCAGCA GTCCGTGTCG CCGCACAGCA ACGGTTCCAG CTCCAGTTCG 
AGCTCTAGCT CCAGCTCCAG TTCGTCATCC TCCTCCACAT CCTCCAACTG CAGCTCCAGC 
TCGGCCAGCA GCTGCCAGTA TTTCCAGTCG CCGCACTCCA CCAGCAACGG CACCAGTGCA 
CCGGCGAGCT CCAGTTCGGG ATCGAACAGC GCCACGCCCC TGCTGGAACT GCAGGTGGAC 
ATTGCTGACT CGGCGCAGCC TCTCAATTTG TCCAAGAAAT CGCCCACGCC GCCGCCCAGC 
AAGCTGCACG CTCTGGTGGC CGCCGCCAAT GCCGTTCAAA GGTATCCCAC ATTGTCCGCC 
GACGTCACAG TGACAGCCTC CAATGGCGGT CCTCCGTCGG CGGCGGCGAG TCCGGCGCCC 
AGCAGCAGTC CGCCGGCGAG TGTGGGCTCC CCCAATCCGG GCCTGAGCGC CGCCGTGCAC 
AAGGTAATGC TGGAGGCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eip75B ( NM_001316451.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001303380.1 Drosophila melanogaster Ecdysone-induced protein 75B (Eip75B), transcript variant G, mRNA.

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CloneID	OFa41376
Clone ID Related Accession (Same CDS sequence)	NM_001316451.1
Accession Version	NM_001316451.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ecdysone-induced protein 75B, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGTTG GCAGTCGGGA GGATCTCTTT GCCCAGGCAA AACATATAGA CGATACCGAA 
CGTTTGATTG GACGAGTTTT AGCTGAATTC GATGGCACCA CAGTGCTGTG CCGCGTTTGC 
GGGGATAAGG CCTCCGGTTT CCATTACGGC GTGCATTCCT GCGAGGGTTG CAAGGGATTC 
TTCCGCCGCT CCATCCAGCA AAAGATCCAG TATCGCCCGT GCACCAAGAA TCAGCAGTGC 
AGCATTCTGC GCATCAATCG CAATCGTTGT CAATATTGCC GCCTGAAAAA GTGCATTGCC 
GTGGGCATGA GTCGCGATGC TGTGCGTTTT GGACGCGTGC CGAAGCGCGA AAAGGCGCGT 
ATCCTGGCGG CCATGCAACA GAGCACCCAG AATCGCGGCC AGCAGCGAGC CCTCGCCACC 
GAGCTGGATG ACCAGCCACG CCTCCTCGCC GCCGTGCTGC GCGCCCACCT CGAGACCTGT 
GAGTTCACCA AGGAGAAGGT CTCGGCGATG CGGCAGCGGG CGCGGGATTG CCCCTCCTAC 
TCCATGCCCA CACTTCTGGC CTGTCCGCTG AACCCCGCCC CTGAACTGCA ATCGGAGCAG 
GAGTTCTCGC AGCGTTTCGC CCACGTAATT CGCGGCGTGA TCGACTTTGC CGGCATGATT 
CCCGGCTTCC AGCTGCTCAC CCAGGACGAT AAGTTCACGC TCCTGAAGGC GGGACTCTTC 
GACGCCCTGT TTGTGCGCCT GATCTGCATG TTTGACTCGT CGATAAACTC AATCATCTGT 
CTAAATGGCC AGGTGATGCG ACGGGATGCG ATCCAGAACG GAGCCAATGC CCGCTTCCTG 
GTGGACTCCA CCTTCAATTT CGCGGAGCGC ATGAACTCGA TGAACCTGAC AGATGCCGAG 
ATAGGCCTGT TCTGCGCCAT CGTTCTGATT ACGCCGGATC GCCCCGGTTT GCGCAACCTG 
GAGCTGATCG AGAAGATGTA CTCGCGACTC AAGGGCTGCC TGCAGTACAT TGTCGCCCAG 
AATAGGCCCG ATCAGCCCGA GTTCCTGGCC AAGTTGCTGG AGACGATGCC CGATCTGCGC 
ACCCTGAGCA CCCTGCACAC CGAGAAACTG GTAGTTTTCC GCACCGAGCA CAAGGAGCTG 
CTGCGCCAGC AGATGTGGTC CATGGAGGAC GGCAACAACA GCGATGGCCA GCAGAACAAG 
TCGCCCTCGG GCAGCTGGGC GGATGCCATG GACGTGGAGG CGGCCAAGAG TCCGCTTGGC 
TCGGTATCGA GCACTGAGTC CGCCGACCTG GACTACGGCA GTCCGAGCAG TTCGCAGCCA 
CAGGGCGTGT CTCTGCCCTC GCCGCCTCAG CAACAGCCCT CGGCTCTGGC CAGCTCGGCT 
CCTCTGCTGG CGGCCACCCT CTCCGGAGGA TGTCCCCTGC GCAACCGGGC CAATTCCGGC 
TCCAGCGGTG ACTCCGGAGC AGCTGAGATG GATATCGTTG GCTCGCACGC ACATCTCACC 
CAGAACGGGC TGACAATCAC GCCGATTGTG CGACACCAGC AGCAGCAACA ACAGCAGCAG 
CAGATCGGAA TACTCAATAA TGCGCATTCC CGCAACTTGA ATGGGGGACA CGCGATGTGC 
CAGCAACAGC AGCAGCACCC ACAACTGCAC CACCACTTGA CAGCCGGAGC TGCCCGCTAC 
AGAAAGCTAG ATTCGCCCAC GGATTCGGGC ATTGAGTCGG GCAACGAGAA GAACGAGTGC 
AAGGCGGTGA GTTCGGGGGG AAGTTCCTCG TGCTCCAGTC CGCGTTCCAG TGTGGATGAT 
GCGCTGGACT GCAGCGATGC CGCCGCCAAT CACAATCAGG TGGTGCAGCA TCCGCAGCTG 
AGTGTGGTGT CCGTGTCACC AGTTCGCTCG CCCCAGCCCT CCACCAGCAG CCATCTGAAG 
CGACAGATTG TGGAGGATAT GCCCGTGCTG AAGCGCGTGC TGCAGGCTCC CCCTCTGTAC 
GATACCAACT CGCTGATGGA CGAGGCCTAC AAGCCGCACA AGAAATTCCG GGCCCTGCGG 
CATCGCGAGT TCGAGACCGC CGAGGCGGAT GCCAGCAGTT CCACTTCCGG CTCGAACAGC 
CTGAGTGCCG GCAGTCCGCG ACAGAGTCCA GTCCCGAACA GTGTGGCCAC GCCCCCGCCA 
TCGGCGGCCA GCGCCGCCGC AGGTAATCCC GCCCAGAGCC AGCTGCACAT GCACCTGACC 
CGCAGCAGCC CCAAGGCCTC GATGGCCAGC TCGCACTCGG TGCTGGCCAA GTCTCTCATG 
GCCGAGCCGC GCATGACGCC CGAGCAGATG AAGCGCAGCG ATATTATCCA AAACTACTTG 
AAGCGCGAGA ACAGCACAGC AGCCAGCAGC ACCACCAATG GCGTGGGCAA CCGCAGTCCC 
AGCAGCAGCT CCACACCGCC GCCATCGGCG GTCCAGAATC AGCAGCGTTG GGGCAGCAGC 
TCGGTGATCA CCACCACCTG CCAGCAGCGC CAGCAGTCCG TGTCGCCGCA CAGCAACGGT 
TCCAGCTCCA GTTCGAGCTC TAGCTCCAGC TCCAGTTCGT CATCCTCCTC CACATCCTCC 
AACTGCAGCT CCAGCTCGGC CAGCAGCTGC CAGTATTTCC AGTCGCCGCA CTCCACCAGC 
AACGGCACCA GTGCACCGGC GAGCTCCAGT TCGGGATCGA ACAGCGCCAC GCCCCTGCTG 
GAACTGCAGG TGGACATTGC TGACTCGGCG CAGCCTCTCA ATTTGTCCAA GAAATCGCCC 
ACGCCGCCGC CCAGCAAGCT GCACGCTCTG GTGGCCGCCG CCAATGCCGT TCAAAGGTAT 
CCCACATTGT CCGCCGACGT CACAGTGACA GCCTCCAATG GCGGTCCTCC GTCGGCGGCG 
GCGAGTCCGG CGCCCAGCAG CAGTCCGCCG GCGAGTGTGG GCTCCCCCAA TCCGGGCCTG 
AGCGCCGCCG TGCACAAGGT AATGCTGGAG GCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303380.1
CDS	618..3593
Translation	MTVGSREDLFAQAKHIDDTERLIGRVLAEFDGTTVLCRVCGDKASGFHYGVHSCEGCKGFFRRSIQQKIQYRPCTKNQQCSILRINRNRCQYCRLKKCIAVGMSRDAVRFGRVPKREKARILAAMQQSTQNRGQQRALATELDDQPRLLAAVLRAHLETCEFTKEKVSAMRQRARDCPSYSMPTLLACPLNPAPELQSEQEFSQRFAHVIRGVIDFAGMIPGFQLLTQDDKFTLLKAGLFDALFVRLICMFDSSINSIICLNGQVMRRDAIQNGANARFLVDSTFNFAERMNSMNLTDAEIGLFCAIVLITPDRPGLRNLELIEKMYSRLKGCLQYIVAQNRPDQPEFLAKLLETMPDLRTLSTLHTEKLVVFRTEHKELLRQQMWSMEDGNNSDGQQNKSPSGSWADAMDVEAAKSPLGSVSSTESADLDYGSPSSSQPQGVSLPSPPQQQPSALASSAPLLAATLSGGCPLRNRANSGSSGDSGAAEMDIVGSHAHLTQNGLTITPIVRHQQQQQQQQQIGILNNAHSRNLNGGHAMCQQQQQHPQLHHHLTAGAARYRKLDSPTDSGIESGNEKNECKAVSSGGSSSCSSPRSSVDDALDCSDAAANHNQVVQHPQLSVVSVSPVRSPQPSTSSHLKRQIVEDMPVLKRVLQAPPLYDTNSLMDEAYKPHKKFRALRHREFETAEADASSSTSGSNSLSAGSPRQSPVPNSVATPPPSAASAAAGNPAQSQLHMHLTRSSPKASMASSHSVLAKSLMAEPRMTPEQMKRSDIIQNYLKRENSTAASSTTNGVGNRSPSSSSTPPPSAVQNQQRWGSSSVITTTCQQRQQSVSPHSNGSSSSSSSSSSSSSSSSSTSSNCSSSSASSCQYFQSPHSTSNGTSAPASSSSGSNSATPLLELQVDIADSAQPLNLSKKSPTPPPSKLHALVAAANAVQRYPTLSADVTVTASNGGPPSAAASPAPSSSPPASVGSPNPGLSAAVHKVMLEA

Target ORF information:

RefSeq Version	NM_001316451.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ecdysone-induced protein 75B (Eip75B), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316451.1

ORF Insert Sequence:

ATGACAGTTG GCAGTCGGGA GGATCTCTTT GCCCAGGCAA AACATATAGA CGATACCGAA 
CGTTTGATTG GACGAGTTTT AGCTGAATTC GATGGCACCA CAGTGCTGTG CCGCGTTTGC 
GGGGATAAGG CCTCCGGTTT CCATTACGGC GTGCATTCCT GCGAGGGTTG CAAGGGATTC 
TTCCGCCGCT CCATCCAGCA AAAGATCCAG TATCGCCCGT GCACCAAGAA TCAGCAGTGC 
AGCATTCTGC GCATCAATCG CAATCGTTGT CAATATTGCC GCCTGAAAAA GTGCATTGCC 
GTGGGCATGA GTCGCGATGC TGTGCGTTTT GGACGCGTGC CGAAGCGCGA AAAGGCGCGT 
ATCCTGGCGG CCATGCAACA GAGCACCCAG AATCGCGGCC AGCAGCGAGC CCTCGCCACC 
GAGCTGGATG ACCAGCCACG CCTCCTCGCC GCCGTGCTGC GCGCCCACCT CGAGACCTGT 
GAGTTCACCA AGGAGAAGGT CTCGGCGATG CGGCAGCGGG CGCGGGATTG CCCCTCCTAC 
TCCATGCCCA CACTTCTGGC CTGTCCGCTG AACCCCGCCC CTGAACTGCA ATCGGAGCAG 
GAGTTCTCGC AGCGTTTCGC CCACGTAATT CGCGGCGTGA TCGACTTTGC CGGCATGATT 
CCCGGCTTCC AGCTGCTCAC CCAGGACGAT AAGTTCACGC TCCTGAAGGC GGGACTCTTC 
GACGCCCTGT TTGTGCGCCT GATCTGCATG TTTGACTCGT CGATAAACTC AATCATCTGT 
CTAAATGGCC AGGTGATGCG ACGGGATGCG ATCCAGAACG GAGCCAATGC CCGCTTCCTG 
GTGGACTCCA CCTTCAATTT CGCGGAGCGC ATGAACTCGA TGAACCTGAC AGATGCCGAG 
ATAGGCCTGT TCTGCGCCAT CGTTCTGATT ACGCCGGATC GCCCCGGTTT GCGCAACCTG 
GAGCTGATCG AGAAGATGTA CTCGCGACTC AAGGGCTGCC TGCAGTACAT TGTCGCCCAG 
AATAGGCCCG ATCAGCCCGA GTTCCTGGCC AAGTTGCTGG AGACGATGCC CGATCTGCGC 
ACCCTGAGCA CCCTGCACAC CGAGAAACTG GTAGTTTTCC GCACCGAGCA CAAGGAGCTG 
CTGCGCCAGC AGATGTGGTC CATGGAGGAC GGCAACAACA GCGATGGCCA GCAGAACAAG 
TCGCCCTCGG GCAGCTGGGC GGATGCCATG GACGTGGAGG CGGCCAAGAG TCCGCTTGGC 
TCGGTATCGA GCACTGAGTC CGCCGACCTG GACTACGGCA GTCCGAGCAG TTCGCAGCCA 
CAGGGCGTGT CTCTGCCCTC GCCGCCTCAG CAACAGCCCT CGGCTCTGGC CAGCTCGGCT 
CCTCTGCTGG CGGCCACCCT CTCCGGAGGA TGTCCCCTGC GCAACCGGGC CAATTCCGGC 
TCCAGCGGTG ACTCCGGAGC AGCTGAGATG GATATCGTTG GCTCGCACGC ACATCTCACC 
CAGAACGGGC TGACAATCAC GCCGATTGTG CGACACCAGC AGCAGCAACA ACAGCAGCAG 
CAGATCGGAA TACTCAATAA TGCGCATTCC CGCAACTTGA ATGGGGGACA CGCGATGTGC 
CAGCAACAGC AGCAGCACCC ACAACTGCAC CACCACTTGA CAGCCGGAGC TGCCCGCTAC 
AGAAAGCTAG ATTCGCCCAC GGATTCGGGC ATTGAGTCGG GCAACGAGAA GAACGAGTGC 
AAGGCGGTGA GTTCGGGGGG AAGTTCCTCG TGCTCCAGTC CGCGTTCCAG TGTGGATGAT 
GCGCTGGACT GCAGCGATGC CGCCGCCAAT CACAATCAGG TGGTGCAGCA TCCGCAGCTG 
AGTGTGGTGT CCGTGTCACC AGTTCGCTCG CCCCAGCCCT CCACCAGCAG CCATCTGAAG 
CGACAGATTG TGGAGGATAT GCCCGTGCTG AAGCGCGTGC TGCAGGCTCC CCCTCTGTAC 
GATACCAACT CGCTGATGGA CGAGGCCTAC AAGCCGCACA AGAAATTCCG GGCCCTGCGG 
CATCGCGAGT TCGAGACCGC CGAGGCGGAT GCCAGCAGTT CCACTTCCGG CTCGAACAGC 
CTGAGTGCCG GCAGTCCGCG ACAGAGTCCA GTCCCGAACA GTGTGGCCAC GCCCCCGCCA 
TCGGCGGCCA GCGCCGCCGC AGGTAATCCC GCCCAGAGCC AGCTGCACAT GCACCTGACC 
CGCAGCAGCC CCAAGGCCTC GATGGCCAGC TCGCACTCGG TGCTGGCCAA GTCTCTCATG 
GCCGAGCCGC GCATGACGCC CGAGCAGATG AAGCGCAGCG ATATTATCCA AAACTACTTG 
AAGCGCGAGA ACAGCACAGC AGCCAGCAGC ACCACCAATG GCGTGGGCAA CCGCAGTCCC 
AGCAGCAGCT CCACACCGCC GCCATCGGCG GTCCAGAATC AGCAGCGTTG GGGCAGCAGC 
TCGGTGATCA CCACCACCTG CCAGCAGCGC CAGCAGTCCG TGTCGCCGCA CAGCAACGGT 
TCCAGCTCCA GTTCGAGCTC TAGCTCCAGC TCCAGTTCGT CATCCTCCTC CACATCCTCC 
AACTGCAGCT CCAGCTCGGC CAGCAGCTGC CAGTATTTCC AGTCGCCGCA CTCCACCAGC 
AACGGCACCA GTGCACCGGC GAGCTCCAGT TCGGGATCGA ACAGCGCCAC GCCCCTGCTG 
GAACTGCAGG TGGACATTGC TGACTCGGCG CAGCCTCTCA ATTTGTCCAA GAAATCGCCC 
ACGCCGCCGC CCAGCAAGCT GCACGCTCTG GTGGCCGCCG CCAATGCCGT TCAAAGGTAT 
CCCACATTGT CCGCCGACGT CACAGTGACA GCCTCCAATG GCGGTCCTCC GTCGGCGGCG 
GCGAGTCCGG CGCCCAGCAG CAGTCCGCCG GCGAGTGTGG GCTCCCCCAA TCCGGGCCTG 
AGCGCCGCCG TGCACAAGGT AATGCTGGAG GCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Eip75B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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