nan cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	nan
Entrez Gene ID	39571
Full Name	nanchung
Synonyms	CG5842,CT18317,DmNan,Dmel\CG5842,NAN,Nan,OCR,TRPV
General protein information	Preferred Names nanchung Names CG5842 gene product from transcript CG5842-RA CG5842-PA CG5842-PB NANCHUNG nan-PA nan-PB transient receptor potential vanilloid
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 70D3-70D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_140439.3	NP_648696.2	nanchung, isoform A
NM_001274904.1	NP_001261833.1	nanchung, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	nan	NP_001261833.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012241	XP_320300.4
Caenorhabditis elegans (roundworm)	ocr-4	NP_501172.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The transient receptor potential channels NOMPC, NANCHUNG, and INACTIVE, but not the dmPIEZO channel, are implicated in mechanotransduction and/or signal amplification for the detection of sound by the larval Cho neurons.
Title: Sound response mediated by the TRP channels NOMPC, NANCHUNG, and INACTIVE in chordotonal organs of Drosophila larvae.

identification of two Drosophila melanogaster transient receptor potential channels needed for sensing humidity: water witch (wtrw), which is required to detect moist air, and nanchung (nan), which is involved in detecting dry air
Title: Drosophila hygrosensation requires the TRP channels water witch and nanchung.

Nan mediates hypo-osmotically activated calcium influx and cation currents; expressed in vivo exclusively in chordotonal neurons and localized to their sensory cilia; essential component of the chordotonal mechanotransducer
Title: A TRPV family ion channel required for hearing in Drosophila.

The following nan gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nan cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18485	NM_140439.3 Latest version!	Drosophila melanogaster nanchung (nan), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa20940	NM_001274904.1 Latest version!	Drosophila melanogaster nanchung (nan), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nan ( NM_140439.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648696.2 Drosophila melanogaster nanchung (nan), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18485
Clone ID Related Accession (Same CDS sequence)	NM_140439.3
Accession Version	NM_140439.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nanchung, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On May 8, 2012 this sequence version replaced NM_140439.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGGAATA CGGAGAGCAA TGTTACCAGC GGGGTGAAGA AGCAGGCCGG AGTCTCCACC 
CAGGCTCTAT ACAAGTTCGT GAATCTCAAG GGCGGTGGAC TCCTGGTGGA CATGATGAAA 
AGAGCCTGTC AAACCAAACA GTTTGCTGAG ATCGATCATG CTATCAAAAC CAAAGTGGAG 
CCCTTTCTGT ACAACAAGGG CGCTGGACGG TATTTTCCCA TCTCGAAGCT CGTACTGCTG 
CGAAATCGGG ATCGCCCACG CACTCGCCAA TTGCCCGAGA TCCGAGCCCT CGAGAATCCT 
GACGACGATT TCAACATCCA TGACTACTGC CCCGAGGTTT CGGAGGCCGA GTATATCTCC 
AATCCTACGG CCTATCGATT CGTCTGCTGG GATCTTAATA TGCGTGGCGC TGTGGGCGAA 
ACTATTCTCC ATTTGTGCCT GCTAAATGCA TCTTCCTTGC ACGCAGATCT CGCCAAAAGA 
TTGCTAAAAT TCTACCCGAA ACTCATCCTG GACATTTACA TGAGCGACGA GTATTATGGA 
GAGAGTGTCC TGCACATTGC CATCGTCAAT GAAGATCCGG CAATGGTGAA GTATCTGCTG 
GATGCCAACG CAGATGTTCA AGAGCGGTGC TGTGGAGCCT TCATGTCGGC GGAGGATACT 
AAGTTTTCTC GCACGGATTC GCCGGATCAC GAGTATGTGG CACTTTGTCC CATGACCAAC 
TACGATGGTT ATGTTTACTG GGGAGAATAC CCATTGAGTT TTGCCGCTTG CCTGTCGCAG 
GAGGAGTGCT TCCGCTTAGT TTTGGCCAGA GGTGCCGATC CCGACTTCCA GGACACCAAC 
GGAAACACCG TTCTCCACAT GCTGGTCATT TACGAAAAGA TCGAAATGTT CGATGTTGGC 
TACGAGGTTG GAACAAACAT ACACATAAAA AACATTCAGA ATCTCACGCC CCTTACTTTG 
GCGGCCAAGT TGGGCAGAGT TGAGATGTTC TTCCACGTGA TGAGCATCGA ACGGGAGATC 
TATTGGCAAT TGGGTAGCAT CACATGTGCC GCATATCCGC TTTTGATGAT AGACACTATA 
AATGAGGAAA CCGGCAATAT CAACAAGGAC TCCGTGCTGA ACTTTGTGGT ATTTGGCGAT 
AAGCTGGAGC ACTTGGAGCT GCTAGATGGG GTGGTCATAG ATCTGCTAAA GACCAAATGG 
GACACGTTCT GCAAATCGCG ATTTTACAAG CAATTTTACA TGTTCGCCCT CTACTTTCTC 
ATCTCGCTGT TCAGTTTTAT ACTGCGTCCA GGTCCGGATG CCAAGGACGA AGACGAGGAT 
GGCGCCAATA GCACTACGGC TAAAAGTGAT CTGTATAGGC AAAATGGATC GGATTCTTAC 
CATCTGCACA GCAAGCGGGC TACAATGACT ACCGAATACA AAACATTTTG GCTTAATTTT 
ACTGAGTACT ACGATCCATC GGAAGTTGAG GTCCTGCCCG CCTGGTGGGA AAGTTATGCT 
CAGTGTCCTT TGATGAATCT CGAATCCGAT TTGGCCAAGC TTCGTATTAT GGCAGAACTG 
TTGAATTTTG TAGGAGCTAT CCTGTATCTG CTGGTTGCTT TGCGAGAAGC TCGATTCCTG 
GGCCTTAAAA TGTTTATCGA AAACTTGATG ACGGCACCTT CGCGAGTTAT GTTTCTGTTT 
TCGTGTGCTT TGATGATGAC TATACCCTGG TTGAGGGTTT CCTGTCTCAC CGAAATAGAT 
GATCACGTCA CCGTTGTGAT AATGCTAACC ACTGCTCCCT ATTTTCTATT CTTTTGTCGC 
GGCTTCAAAA CTGTCGGTCC CTTCGTAGTG ATGATATACC GCATGGTCAT GGGAGATCTG 
CTCCGATTCG TCTCCATCTA CTTGGTGTTC GTCATGGGTT TCTCCCAGGC ATTTTACATA 
ATTTTTCTAA CCTTCGACAA CCCATCGTCA CCAGAGGATC AGGACGCGGA ATCCAACCCA 
ATGCCCTCGC CCATGGAATC GATAGTGGCC ATGTTCCTAA TGTCGCTAAC AAATTTCGGT 
GACTACTATG GAGCGATGGT GTCTACGCAG CACGAATACG AGGCGAAGAT CCTCTTCTTT 
CTGTTCATGG TGATCGTGAG TGTTCTGCTG GTGAACATGT TGATCGCCAT GATGGGTAAT 
ACGTATCAGA AGATCGCCGA GATCCGAAAC GAGTGGCAGC GCCAATGGGC CAGGATCGTC 
CTCGTCGTGG AGCGAAGTGT TCCTCCCGCC GAGCGGTTGA AGAACTTTAT GCAGTACAGT 
CAGTCGATGT CGGATGGCAG AAGGGCACTG GTCCTCCGGT TGAATATGAC TGAGGAAAAG 
GAGGAGATGA AGGAGGTGCA GGAGATGAAG CGCATCCATC AACGGTTCGC CAAGAAGCGG 
CAAATGGAGC GCGAGGCTCG CGCCCTTCGA CGTCAACAGG AGTACGAGAA GTTCTTCGGC 
ACCGCCCCCA AATCGGAATG TTCCGATAAC AACAACTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648696.2
CDS	227..2728
Translation	MGNTESNVTSGVKKQAGVSTQALYKFVNLKGGGLLVDMMKRACQTKQFAEIDHAIKTKVEPFLYNKGAGRYFPISKLVLLRNRDRPRTRQLPEIRALENPDDDFNIHDYCPEVSEAEYISNPTAYRFVCWDLNMRGAVGETILHLCLLNASSLHADLAKRLLKFYPKLILDIYMSDEYYGESVLHIAIVNEDPAMVKYLLDANADVQERCCGAFMSAEDTKFSRTDSPDHEYVALCPMTNYDGYVYWGEYPLSFAACLSQEECFRLVLARGADPDFQDTNGNTVLHMLVIYEKIEMFDVGYEVGTNIHIKNIQNLTPLTLAAKLGRVEMFFHVMSIEREIYWQLGSITCAAYPLLMIDTINEETGNINKDSVLNFVVFGDKLEHLELLDGVVIDLLKTKWDTFCKSRFYKQFYMFALYFLISLFSFILRPGPDAKDEDEDGANSTTAKSDLYRQNGSDSYHLHSKRATMTTEYKTFWLNFTEYYDPSEVEVLPAWWESYAQCPLMNLESDLAKLRIMAELLNFVGAILYLLVALREARFLGLKMFIENLMTAPSRVMFLFSCALMMTIPWLRVSCLTEIDDHVTVVIMLTTAPYFLFFCRGFKTVGPFVVMIYRMVMGDLLRFVSIYLVFVMGFSQAFYIIFLTFDNPSSPEDQDAESNPMPSPMESIVAMFLMSLTNFGDYYGAMVSTQHEYEAKILFFLFMVIVSVLLVNMLIAMMGNTYQKIAEIRNEWQRQWARIVLVVERSVPPAERLKNFMQYSQSMSDGRRALVLRLNMTEEKEEMKEVQEMKRIHQRFAKKRQMEREARALRRQQEYEKFFGTAPKSECSDNNNF

Target ORF information:

RefSeq Version	NM_140439.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nanchung (nan), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140439.3

ORF Insert Sequence:

ATGGGGAATA CGGAGAGCAA TGTTACCAGC GGGGTGAAGA AGCAGGCCGG AGTCTCCACC 
CAGGCTCTAT ACAAGTTCGT GAATCTCAAG GGCGGTGGAC TCCTGGTGGA CATGATGAAA 
AGAGCCTGTC AAACCAAACA GTTTGCTGAG ATCGATCATG CTATCAAAAC CAAAGTGGAG 
CCCTTTCTGT ACAACAAGGG CGCTGGACGG TATTTTCCCA TCTCGAAGCT CGTACTGCTG 
CGAAATCGGG ATCGCCCACG CACTCGCCAA TTGCCCGAGA TCCGAGCCCT CGAGAATCCT 
GACGACGATT TCAACATCCA TGACTACTGC CCCGAGGTTT CGGAGGCCGA GTATATCTCC 
AATCCTACGG CCTATCGATT CGTCTGCTGG GATCTTAATA TGCGTGGCGC TGTGGGCGAA 
ACTATTCTCC ATTTGTGCCT GCTAAATGCA TCTTCCTTGC ACGCAGATCT CGCCAAAAGA 
TTGCTAAAAT TCTACCCGAA ACTCATCCTG GACATTTACA TGAGCGACGA GTATTATGGA 
GAGAGTGTCC TGCACATTGC CATCGTCAAT GAAGATCCGG CAATGGTGAA GTATCTGCTG 
GATGCCAACG CAGATGTTCA AGAGCGGTGC TGTGGAGCCT TCATGTCGGC GGAGGATACT 
AAGTTTTCTC GCACGGATTC GCCGGATCAC GAGTATGTGG CACTTTGTCC CATGACCAAC 
TACGATGGTT ATGTTTACTG GGGAGAATAC CCATTGAGTT TTGCCGCTTG CCTGTCGCAG 
GAGGAGTGCT TCCGCTTAGT TTTGGCCAGA GGTGCCGATC CCGACTTCCA GGACACCAAC 
GGAAACACCG TTCTCCACAT GCTGGTCATT TACGAAAAGA TCGAAATGTT CGATGTTGGC 
TACGAGGTTG GAACAAACAT ACACATAAAA AACATTCAGA ATCTCACGCC CCTTACTTTG 
GCGGCCAAGT TGGGCAGAGT TGAGATGTTC TTCCACGTGA TGAGCATCGA ACGGGAGATC 
TATTGGCAAT TGGGTAGCAT CACATGTGCC GCATATCCGC TTTTGATGAT AGACACTATA 
AATGAGGAAA CCGGCAATAT CAACAAGGAC TCCGTGCTGA ACTTTGTGGT ATTTGGCGAT 
AAGCTGGAGC ACTTGGAGCT GCTAGATGGG GTGGTCATAG ATCTGCTAAA GACCAAATGG 
GACACGTTCT GCAAATCGCG ATTTTACAAG CAATTTTACA TGTTCGCCCT CTACTTTCTC 
ATCTCGCTGT TCAGTTTTAT ACTGCGTCCA GGTCCGGATG CCAAGGACGA AGACGAGGAT 
GGCGCCAATA GCACTACGGC TAAAAGTGAT CTGTATAGGC AAAATGGATC GGATTCTTAC 
CATCTGCACA GCAAGCGGGC TACAATGACT ACCGAATACA AAACATTTTG GCTTAATTTT 
ACTGAGTACT ACGATCCATC GGAAGTTGAG GTCCTGCCCG CCTGGTGGGA AAGTTATGCT 
CAGTGTCCTT TGATGAATCT CGAATCCGAT TTGGCCAAGC TTCGTATTAT GGCAGAACTG 
TTGAATTTTG TAGGAGCTAT CCTGTATCTG CTGGTTGCTT TGCGAGAAGC TCGATTCCTG 
GGCCTTAAAA TGTTTATCGA AAACTTGATG ACGGCACCTT CGCGAGTTAT GTTTCTGTTT 
TCGTGTGCTT TGATGATGAC TATACCCTGG TTGAGGGTTT CCTGTCTCAC CGAAATAGAT 
GATCACGTCA CCGTTGTGAT AATGCTAACC ACTGCTCCCT ATTTTCTATT CTTTTGTCGC 
GGCTTCAAAA CTGTCGGTCC CTTCGTAGTG ATGATATACC GCATGGTCAT GGGAGATCTG 
CTCCGATTCG TCTCCATCTA CTTGGTGTTC GTCATGGGTT TCTCCCAGGC ATTTTACATA 
ATTTTTCTAA CCTTCGACAA CCCATCGTCA CCAGAGGATC AGGACGCGGA ATCCAACCCA 
ATGCCCTCGC CCATGGAATC GATAGTGGCC ATGTTCCTAA TGTCGCTAAC AAATTTCGGT 
GACTACTATG GAGCGATGGT GTCTACGCAG CACGAATACG AGGCGAAGAT CCTCTTCTTT 
CTGTTCATGG TGATCGTGAG TGTTCTGCTG GTGAACATGT TGATCGCCAT GATGGGTAAT 
ACGTATCAGA AGATCGCCGA GATCCGAAAC GAGTGGCAGC GCCAATGGGC CAGGATCGTC 
CTCGTCGTGG AGCGAAGTGT TCCTCCCGCC GAGCGGTTGA AGAACTTTAT GCAGTACAGT 
CAGTCGATGT CGGATGGCAG AAGGGCACTG GTCCTCCGGT TGAATATGAC TGAGGAAAAG 
GAGGAGATGA AGGAGGTGCA GGAGATGAAG CGCATCCATC AACGGTTCGC CAAGAAGCGG 
CAAATGGAGC GCGAGGCTCG CGCCCTTCGA CGTCAACAGG AGTACGAGAA GTTCTTCGGC 
ACCGCCCCCA AATCGGAATG TTCCGATAAC AACAACTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nan ( NM_001274904.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261833.1 Drosophila melanogaster nanchung (nan), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa20940
Clone ID Related Accession (Same CDS sequence)	NM_001274904.1
Accession Version	NM_001274904.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nanchung, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGGAATA CGGAGAGCAA TGTTACCAGC GGGGTGAAGA AGCAGGCCGG AGTCTCCACC 
CAGGCTCTAT ACAAGTTCGT GAATCTCAAG GGCGGTGGAC TCCTGGTGGA CATGATGAAA 
AGAGCCTGTC AAACCAAACA GTTTGCTGAG ATCGATCATG CTATCAAAAC CAAAGTGGAG 
CCCTTTCTGT ACAACAAGGG CGCTGGACGG TATTTTCCCA TCTCGAAGCT CGTACTGCTG 
CGAAATCGGG ATCGCCCACG CACTCGCCAA TTGCCCGAGA TCCGAGCCCT CGAGAATCCT 
GACGACGATT TCAACATCCA TGACTACTGC CCCGAGGTTT CGGAGGCCGA GTATATCTCC 
AATCCTACGG CCTATCGATT CGTCTGCTGG GATCTTAATA TGCGTGGCGC TGTGGGCGAA 
ACTATTCTCC ATTTGTGCCT GCTAAATGCA TCTTCCTTGC ACGCAGATCT CGCCAAAAGA 
TTGCTAAAAT TCTACCCGAA ACTCATCCTG GACATTTACA TGAGCGACGA GTATTATGGA 
GAGAGTGTCC TGCACATTGC CATCGTCAAT GAAGATCCGG CAATGGTGAA GTATCTGCTG 
GATGCCAACG CAGATGTTCA AGAGCGGTGC TGTGGAGCCT TCATGTCGGC GGAGGATACT 
AAGTTTTCTC GCACGGATTC GCCGGATCAC GAGTATGTGG CACTTTGTCC CATGACCAAC 
TACGATGGTT ATGTTTACTG GGGAGAATAC CCATTGAGTT TTGCCGCTTG CCTGTCGCAG 
GAGGAGTGCT TCCGCTTAGT TTTGGCCAGA GGTGCCGATC CCGACTTCCA GGACACCAAC 
GGAAACACCG TTCTCCACAT GCTGGTCATT TACGAAAAGA TCGAAATGTT CGATGTTGGC 
TACGAGGTTG GAACAAACAT ACACATAAAA AACATTCAGA ATCTCACGCC CCTTACTTTG 
GCGGCCAAGT TGGGCAGAGT TGAGATGTTC TTCCACGTGA TGAGCATCGA ACGGGAGATC 
TATTGGCAAT TGGGTAGCAT CACATGTGCC GCATATCCGC TTTTGATGAT AGACACTATA 
AATGAGGAAA CCGGCAATAT CAACAAGGAC TCCGTGCTGA ACTTTGTGGT ATTTGGCGAT 
AAGCTGGAGC ACTTGGAGCT GCTAGATGGG GTGGTCATAG ATCTGCTAAA GACCAAATGG 
GACACGTTCT GCAAATCGCG ATTTTACAAG CAATTTTACA TGTTCGCCCT CTACTTTCTC 
ATCTCGCTGT TCAGTTTTAT ACTGCGTCCA GGTCCGGATG CCAAGGACGA AGACGAGGAT 
GGCGCCAATA GCACTACGGC TAAAAGTGAT CTGTATAGGC AAAATGGATC GGATTCTTAC 
CATCTGCACA GCAAGCGGGC TACAATGACT ACCGAATACA AAACATTTTG GCTTAATTTT 
ACTGAGTACT ACGATCCATC GGAAGTTGAG GTCCTGCCCG CCTGGTGGGA AAGTTATGCT 
CAGTGTCCTT TGATGAATCT CGAATCCGAT TTGGCCAAGC TTCGTATTAT GGCAGAACTG 
TTGAATTTTG TAGGAGCTAT CCTGTATCTG CTGGTTGCTT TGCGAGAAGC TCGATTCCTG 
GGCCTTAAAA TGTTTATCGA AAACTTGATG ACGGCACCTT CGCGAGTTAT GTTTCTGTTT 
TCGTGTGCTT TGATGATGAC TATACCCTGG TTGAGGGTTT CCTGTCTCAC CGAAATAGAT 
GATCACGTCA CCGTTGTGAT AATGCTAACC ACTGCTCCCT ATTTTCTATT CTTTTGTCGC 
GGCTTCAAAA CTGTCGGTCC CTTCGTAGTG ATGATATACC GCATGGTCAT GGGAGATCTG 
CTCCGATTCG TCTCCATCTA CTTGGTGTTC GTCATGGGTT TCTCCCAGGC ATTTTACATA 
ATTTTTCTAA CCTTCGACAA CCCATCGTCA CCAGAGGATC AGGACGCGGA ATCCAACCCA 
ATGCCCTCGC CCATGGAATC GATAGTGGCC ATGTTCCTAA TGTCGCTAAC AAATTTCGGT 
GACTACTATG GAGCGATGGT GTCTACGCAG CACGAATACG AGGCGAAGAT CCTCTTCTTT 
CTGTTCATGG TGATCGTGAG TGTTCTGCTG GTGAACATGT TGATCGCCAT GATGGGTAAT 
ACGTATCAGA AGATCGCCGA GATCCGAAAC GAGTGGCAGC GCCAATGGGC CAGGATCGTC 
CTCGTCGTGG AGCGAAGTGT TCCTCCCGCC GAGCGGTTGA AGAACTTTAT GCAGTACAGT 
CAGTCGATGT CGGATGGCAG AAGGGCACTG GTCCTCCGGT TGAATATGAC TGAGGAGGAA 
AAGGAGGAGA TGAAGGAGGT GCAGGAGATG AAGCGCATCC ATCAACGGTT CGCCAAGAAG 
CGGCAAATGG AGCGCGAGGC TCGCGCCCTT CGACGTCAAC AGGAGTACGA GAAGTTCTTC 
GGCACCGCCC CCAAATCGGA ATGTTCCGAT AACAACAACT TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261833.1
CDS	227..2731
Translation	MGNTESNVTSGVKKQAGVSTQALYKFVNLKGGGLLVDMMKRACQTKQFAEIDHAIKTKVEPFLYNKGAGRYFPISKLVLLRNRDRPRTRQLPEIRALENPDDDFNIHDYCPEVSEAEYISNPTAYRFVCWDLNMRGAVGETILHLCLLNASSLHADLAKRLLKFYPKLILDIYMSDEYYGESVLHIAIVNEDPAMVKYLLDANADVQERCCGAFMSAEDTKFSRTDSPDHEYVALCPMTNYDGYVYWGEYPLSFAACLSQEECFRLVLARGADPDFQDTNGNTVLHMLVIYEKIEMFDVGYEVGTNIHIKNIQNLTPLTLAAKLGRVEMFFHVMSIEREIYWQLGSITCAAYPLLMIDTINEETGNINKDSVLNFVVFGDKLEHLELLDGVVIDLLKTKWDTFCKSRFYKQFYMFALYFLISLFSFILRPGPDAKDEDEDGANSTTAKSDLYRQNGSDSYHLHSKRATMTTEYKTFWLNFTEYYDPSEVEVLPAWWESYAQCPLMNLESDLAKLRIMAELLNFVGAILYLLVALREARFLGLKMFIENLMTAPSRVMFLFSCALMMTIPWLRVSCLTEIDDHVTVVIMLTTAPYFLFFCRGFKTVGPFVVMIYRMVMGDLLRFVSIYLVFVMGFSQAFYIIFLTFDNPSSPEDQDAESNPMPSPMESIVAMFLMSLTNFGDYYGAMVSTQHEYEAKILFFLFMVIVSVLLVNMLIAMMGNTYQKIAEIRNEWQRQWARIVLVVERSVPPAERLKNFMQYSQSMSDGRRALVLRLNMTEEEKEEMKEVQEMKRIHQRFAKKRQMEREARALRRQQEYEKFFGTAPKSECSDNNNF

Target ORF information:

RefSeq Version	NM_001274904.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nanchung (nan), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274904.1

ORF Insert Sequence:

ATGGGGAATA CGGAGAGCAA TGTTACCAGC GGGGTGAAGA AGCAGGCCGG AGTCTCCACC 
CAGGCTCTAT ACAAGTTCGT GAATCTCAAG GGCGGTGGAC TCCTGGTGGA CATGATGAAA 
AGAGCCTGTC AAACCAAACA GTTTGCTGAG ATCGATCATG CTATCAAAAC CAAAGTGGAG 
CCCTTTCTGT ACAACAAGGG CGCTGGACGG TATTTTCCCA TCTCGAAGCT CGTACTGCTG 
CGAAATCGGG ATCGCCCACG CACTCGCCAA TTGCCCGAGA TCCGAGCCCT CGAGAATCCT 
GACGACGATT TCAACATCCA TGACTACTGC CCCGAGGTTT CGGAGGCCGA GTATATCTCC 
AATCCTACGG CCTATCGATT CGTCTGCTGG GATCTTAATA TGCGTGGCGC TGTGGGCGAA 
ACTATTCTCC ATTTGTGCCT GCTAAATGCA TCTTCCTTGC ACGCAGATCT CGCCAAAAGA 
TTGCTAAAAT TCTACCCGAA ACTCATCCTG GACATTTACA TGAGCGACGA GTATTATGGA 
GAGAGTGTCC TGCACATTGC CATCGTCAAT GAAGATCCGG CAATGGTGAA GTATCTGCTG 
GATGCCAACG CAGATGTTCA AGAGCGGTGC TGTGGAGCCT TCATGTCGGC GGAGGATACT 
AAGTTTTCTC GCACGGATTC GCCGGATCAC GAGTATGTGG CACTTTGTCC CATGACCAAC 
TACGATGGTT ATGTTTACTG GGGAGAATAC CCATTGAGTT TTGCCGCTTG CCTGTCGCAG 
GAGGAGTGCT TCCGCTTAGT TTTGGCCAGA GGTGCCGATC CCGACTTCCA GGACACCAAC 
GGAAACACCG TTCTCCACAT GCTGGTCATT TACGAAAAGA TCGAAATGTT CGATGTTGGC 
TACGAGGTTG GAACAAACAT ACACATAAAA AACATTCAGA ATCTCACGCC CCTTACTTTG 
GCGGCCAAGT TGGGCAGAGT TGAGATGTTC TTCCACGTGA TGAGCATCGA ACGGGAGATC 
TATTGGCAAT TGGGTAGCAT CACATGTGCC GCATATCCGC TTTTGATGAT AGACACTATA 
AATGAGGAAA CCGGCAATAT CAACAAGGAC TCCGTGCTGA ACTTTGTGGT ATTTGGCGAT 
AAGCTGGAGC ACTTGGAGCT GCTAGATGGG GTGGTCATAG ATCTGCTAAA GACCAAATGG 
GACACGTTCT GCAAATCGCG ATTTTACAAG CAATTTTACA TGTTCGCCCT CTACTTTCTC 
ATCTCGCTGT TCAGTTTTAT ACTGCGTCCA GGTCCGGATG CCAAGGACGA AGACGAGGAT 
GGCGCCAATA GCACTACGGC TAAAAGTGAT CTGTATAGGC AAAATGGATC GGATTCTTAC 
CATCTGCACA GCAAGCGGGC TACAATGACT ACCGAATACA AAACATTTTG GCTTAATTTT 
ACTGAGTACT ACGATCCATC GGAAGTTGAG GTCCTGCCCG CCTGGTGGGA AAGTTATGCT 
CAGTGTCCTT TGATGAATCT CGAATCCGAT TTGGCCAAGC TTCGTATTAT GGCAGAACTG 
TTGAATTTTG TAGGAGCTAT CCTGTATCTG CTGGTTGCTT TGCGAGAAGC TCGATTCCTG 
GGCCTTAAAA TGTTTATCGA AAACTTGATG ACGGCACCTT CGCGAGTTAT GTTTCTGTTT 
TCGTGTGCTT TGATGATGAC TATACCCTGG TTGAGGGTTT CCTGTCTCAC CGAAATAGAT 
GATCACGTCA CCGTTGTGAT AATGCTAACC ACTGCTCCCT ATTTTCTATT CTTTTGTCGC 
GGCTTCAAAA CTGTCGGTCC CTTCGTAGTG ATGATATACC GCATGGTCAT GGGAGATCTG 
CTCCGATTCG TCTCCATCTA CTTGGTGTTC GTCATGGGTT TCTCCCAGGC ATTTTACATA 
ATTTTTCTAA CCTTCGACAA CCCATCGTCA CCAGAGGATC AGGACGCGGA ATCCAACCCA 
ATGCCCTCGC CCATGGAATC GATAGTGGCC ATGTTCCTAA TGTCGCTAAC AAATTTCGGT 
GACTACTATG GAGCGATGGT GTCTACGCAG CACGAATACG AGGCGAAGAT CCTCTTCTTT 
CTGTTCATGG TGATCGTGAG TGTTCTGCTG GTGAACATGT TGATCGCCAT GATGGGTAAT 
ACGTATCAGA AGATCGCCGA GATCCGAAAC GAGTGGCAGC GCCAATGGGC CAGGATCGTC 
CTCGTCGTGG AGCGAAGTGT TCCTCCCGCC GAGCGGTTGA AGAACTTTAT GCAGTACAGT 
CAGTCGATGT CGGATGGCAG AAGGGCACTG GTCCTCCGGT TGAATATGAC TGAGGAGGAA 
AAGGAGGAGA TGAAGGAGGT GCAGGAGATG AAGCGCATCC ATCAACGGTT CGCCAAGAAG 
CGGCAAATGG AGCGCGAGGC TCGCGCCCTT CGACGTCAAC AGGAGTACGA GAAGTTCTTC 
GGCACCGCCC CCAAATCGGA ATGTTCCGAT AACAACAACT TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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nan cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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