D cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	D
Entrez Gene ID	39570
Full Name	Dichaete
Synonyms	CG5893,DM10,DM23,DM33,DM36,DM63,Dmel\CG5893,SOX70,SOX70D,SOXB2.1,SOXDP,Sox B2-1,Sox70,Sox70D,Sry-rDM10,Sry-rDM23,Sry-rDM33,Sry-rDM36,Sry-rDM63,delta,fish,l(3)LG9,loD
General protein information	Preferred Names Dichaete Names CG5893 gene product from transcript CG5893-RA CG5893-PA CG5893-PB CG5893-PC CG5893-PD D-PA D-PB D-PC D-PD Fish-Hook Sox box protein 70D Sry-related DM10 Sry-related DM23 Sry-related DM33 Sry-related DM36 Sry-related DM63 dachs delta dichaete fish hook lethal of dichaete
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 70D3-70D3\|3-40.7 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_079342.3	NP_524066.1	dichaete, isoform A
NM_001274902.1	NP_001261831.1	dichaete, isoform C
NM_001274903.1	NP_001261832.1	dichaete, isoform D
NM_001274901.1	NP_001261830.1	dichaete, isoform B

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010919	XP_001688246.1
Drosophila melanogaster (fruit fly)	D	NP_524066.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Analysis of the functional relationship between SoxNeuro and Dichaete uncovers evidence for redundant and independent functions for each protein, along with unexpected examples of compensation and interdependency.
Title: SoxNeuro orchestrates central nervous system specification and differentiation in Drosophila and is only partially redundant with Dichaete.

Dichaete acts as a transcriptional hub, controlling multiple regulatory pathways during CNS development.
Title: The role of Dichaete in transcriptional regulation during Drosophila embryonic development.

These results suggest noncell autonomous functions of Dichaete in projection neuron differentiation as well as a potential role for Dichaete-expressing local interneurons
Title: The sox gene Dichaete is expressed in local interneurons and functions in development of the Drosophila adult olfactory circuit.

the early neurectoderm we implicate Dichaete in the lateral inhibition pathway and show that Dichaete acts to repress the proneural gene asense
Title: Identifying targets of the Sox domain protein Dichaete in the Drosophila CNS via targeted expression of dominant negative proteins.

Data show that Dichaete appears to be a novel phosphorylation substrate for Pelle and may function in a Pelle-dependent signaling pathway during oogenesis.
Title: Drosophila Pelle phosphorylates Dichaete protein and influences its subcellular distribution in developing oocytes.

The following D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18484	NM_001274901.1 Latest version!	Drosophila melanogaster dichaete, transcript variant B (D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa18484	NM_001274903.1 Latest version!	Drosophila melanogaster dichaete, transcript variant D (D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa18484	NM_001274902.1 Latest version!	Drosophila melanogaster dichaete, transcript variant C (D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa18484	NM_079342.3 Latest version!	Drosophila melanogaster dichaete, transcript variant A (D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

D ( NM_079342.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524066.1 Drosophila melanogaster dichaete, transcript variant A (D), mRNA.

Price & Availability↑

CloneID	OFa18484
Clone ID Related Accession (Same CDS sequence)	NM_079342.3 , NM_001274902.1 , NM_001274903.1 , NM_001274901.1
Accession Version	NM_079342.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dichaete, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079342.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCACCT TATCGACACA CCCCAATTAC GGTTTCCATT TGGGTCAGGC CCAAGGCTTG 
GAGGACTACG CACCGCAAAG TCAGCTCCAG CTGAGTCCCG GCATGGACAT GGATATCAAG 
CGTGTGCTGC ACTACTCCCA AAGCCTGGCA GCCATGGGCG GATCGCCCAA TGGACCCGCC 
GGCCAGGGTG TGAACGGATC CTCGGGCATG GGTCACCACA TGTCCAGTCA CATGACCCCG 
CACCACATGC ACCAGGCGGT CTCCGCCCAA CAGACCTTGT CGCCCAACAG TTCCATCGGA 
TCCGCCGGCA GTCTGGGTAG CCAGAGCAGC CTGGGCAGCA ATGGCAGTGG TCTCAACTCC 
AGTTCGGGTC ACCAGTCCGC CGGCATGCAC AGTTTGGCCA CATCGCCGGG ACAAGAGGGT 
CACATCAAGC GTCCAATGAA CGCGTTCATG GTCTGGTCCC GCCTGCAGCG TCGTCAGATC 
GCCAAGGATA ACCCCAAGAT GCACAACTCT GAGATCTCCA AGCGCCTGGG TGCCGAGTGG 
AAGTTGCTGG CCGAGTCGGA GAAACGACCA TTCATCGATG AGGCCAAGCG CTTGAGGGCC 
CTGCACATGA AGGAGCACCC GGACTACAAG TACCGGCCAC GCCGCAAGCC CAAGAACCCA 
CTGACCGCTG GACCCCAGGG TGGACTGCAG ATGCAGGCCG GCGGCATGGG TCAACAGAAG 
TTGGGAGCTG GACCGGGAGC TGGAGCCGGA GGCTACAATC CATTCCATCA ACTACCGCCA 
TACTTTGCAC CCTCACACCA TCTGGATCAG GGTTATCCAG TGCCGTACTT CGGTGGATTT 
GACCCACTGG CTCTATCGAA ATTGCATCAA TCCCAGGCAG CGGCGGCGGC GGCGGTCAAC 
AATCAGGGTC AGCAGCAAGG ACAAGCCCCG CCCCAATTGC CGCCCACCTC GCTGAGCAGC 
TTCTACTCGG GCATTTATTC CGGCATCTCG GCACCTTCGC TGTACGCCGC ACACTCCGCC 
AATGCCGCCG GACTCTACCC ATCCTCGTCC ACCTCCTCGC CGGGATCTTC GCCCGGAACC 
ATCACGCCAA ATGGTATGGA TGGTTCCATG GACAGTGCCC TGAGGCGACC GGTTCCGGTG 
CTCTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524066.1
CDS	1207..2355
Translation	MATLSTHPNYGFHLGQAQGLEDYAPQSQLQLSPGMDMDIKRVLHYSQSLAAMGGSPNGPAGQGVNGSSGMGHHMSSHMTPHHMHQAVSAQQTLSPNSSIGSAGSLGSQSSLGSNGSGLNSSSGHQSAGMHSLATSPGQEGHIKRPMNAFMVWSRLQRRQIAKDNPKMHNSEISKRLGAEWKLLAESEKRPFIDEAKRLRALHMKEHPDYKYRPRRKPKNPLTAGPQGGLQMQAGGMGQQKLGAGPGAGAGGYNPFHQLPPYFAPSHHLDQGYPVPYFGGFDPLALSKLHQSQAAAAAAVNNQGQQQGQAPPQLPPTSLSSFYSGIYSGISAPSLYAAHSANAAGLYPSSSTSSPGSSPGTITPNGMDGSMDSALRRPVPVLY

Target ORF information:

RefSeq Version	NM_079342.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dichaete, transcript variant A (D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079342.3

ORF Insert Sequence:

ATGGCCACCT TATCGACACA CCCCAATTAC GGTTTCCATT TGGGTCAGGC CCAAGGCTTG 
GAGGACTACG CACCGCAAAG TCAGCTCCAG CTGAGTCCCG GCATGGACAT GGATATCAAG 
CGTGTGCTGC ACTACTCCCA AAGCCTGGCA GCCATGGGCG GATCGCCCAA TGGACCCGCC 
GGCCAGGGTG TGAACGGATC CTCGGGCATG GGTCACCACA TGTCCAGTCA CATGACCCCG 
CACCACATGC ACCAGGCGGT CTCCGCCCAA CAGACCTTGT CGCCCAACAG TTCCATCGGA 
TCCGCCGGCA GTCTGGGTAG CCAGAGCAGC CTGGGCAGCA ATGGCAGTGG TCTCAACTCC 
AGTTCGGGTC ACCAGTCCGC CGGCATGCAC AGTTTGGCCA CATCGCCGGG ACAAGAGGGT 
CACATCAAGC GTCCAATGAA CGCGTTCATG GTCTGGTCCC GCCTGCAGCG TCGTCAGATC 
GCCAAGGATA ACCCCAAGAT GCACAACTCT GAGATCTCCA AGCGCCTGGG TGCCGAGTGG 
AAGTTGCTGG CCGAGTCGGA GAAACGACCA TTCATCGATG AGGCCAAGCG CTTGAGGGCC 
CTGCACATGA AGGAGCACCC GGACTACAAG TACCGGCCAC GCCGCAAGCC CAAGAACCCA 
CTGACCGCTG GACCCCAGGG TGGACTGCAG ATGCAGGCCG GCGGCATGGG TCAACAGAAG 
TTGGGAGCTG GACCGGGAGC TGGAGCCGGA GGCTACAATC CATTCCATCA ACTACCGCCA 
TACTTTGCAC CCTCACACCA TCTGGATCAG GGTTATCCAG TGCCGTACTT CGGTGGATTT 
GACCCACTGG CTCTATCGAA ATTGCATCAA TCCCAGGCAG CGGCGGCGGC GGCGGTCAAC 
AATCAGGGTC AGCAGCAAGG ACAAGCCCCG CCCCAATTGC CGCCCACCTC GCTGAGCAGC 
TTCTACTCGG GCATTTATTC CGGCATCTCG GCACCTTCGC TGTACGCCGC ACACTCCGCC 
AATGCCGCCG GACTCTACCC ATCCTCGTCC ACCTCCTCGC CGGGATCTTC GCCCGGAACC 
ATCACGCCAA ATGGTATGGA TGGTTCCATG GACAGTGCCC TGAGGCGACC GGTTCCGGTG 
CTCTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

D ( NM_001274902.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261831.1 Drosophila melanogaster dichaete, transcript variant C (D), mRNA.

Price & Availability↑

CloneID	OFa18484
Clone ID Related Accession (Same CDS sequence)	NM_079342.3 , NM_001274902.1 , NM_001274903.1 , NM_001274901.1
Accession Version	NM_001274902.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dichaete, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCACCT TATCGACACA CCCCAATTAC GGTTTCCATT TGGGTCAGGC CCAAGGCTTG 
GAGGACTACG CACCGCAAAG TCAGCTCCAG CTGAGTCCCG GCATGGACAT GGATATCAAG 
CGTGTGCTGC ACTACTCCCA AAGCCTGGCA GCCATGGGCG GATCGCCCAA TGGACCCGCC 
GGCCAGGGTG TGAACGGATC CTCGGGCATG GGTCACCACA TGTCCAGTCA CATGACCCCG 
CACCACATGC ACCAGGCGGT CTCCGCCCAA CAGACCTTGT CGCCCAACAG TTCCATCGGA 
TCCGCCGGCA GTCTGGGTAG CCAGAGCAGC CTGGGCAGCA ATGGCAGTGG TCTCAACTCC 
AGTTCGGGTC ACCAGTCCGC CGGCATGCAC AGTTTGGCCA CATCGCCGGG ACAAGAGGGT 
CACATCAAGC GTCCAATGAA CGCGTTCATG GTCTGGTCCC GCCTGCAGCG TCGTCAGATC 
GCCAAGGATA ACCCCAAGAT GCACAACTCT GAGATCTCCA AGCGCCTGGG TGCCGAGTGG 
AAGTTGCTGG CCGAGTCGGA GAAACGACCA TTCATCGATG AGGCCAAGCG CTTGAGGGCC 
CTGCACATGA AGGAGCACCC GGACTACAAG TACCGGCCAC GCCGCAAGCC CAAGAACCCA 
CTGACCGCTG GACCCCAGGG TGGACTGCAG ATGCAGGCCG GCGGCATGGG TCAACAGAAG 
TTGGGAGCTG GACCGGGAGC TGGAGCCGGA GGCTACAATC CATTCCATCA ACTACCGCCA 
TACTTTGCAC CCTCACACCA TCTGGATCAG GGTTATCCAG TGCCGTACTT CGGTGGATTT 
GACCCACTGG CTCTATCGAA ATTGCATCAA TCCCAGGCAG CGGCGGCGGC GGCGGTCAAC 
AATCAGGGTC AGCAGCAAGG ACAAGCCCCG CCCCAATTGC CGCCCACCTC GCTGAGCAGC 
TTCTACTCGG GCATTTATTC CGGCATCTCG GCACCTTCGC TGTACGCCGC ACACTCCGCC 
AATGCCGCCG GACTCTACCC ATCCTCGTCC ACCTCCTCGC CGGGATCTTC GCCCGGAACC 
ATCACGCCAA ATGGTATGGA TGGTTCCATG GACAGTGCCC TGAGGCGACC GGTTCCGGTG 
CTCTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261831.1
CDS	200..1348
Translation	MATLSTHPNYGFHLGQAQGLEDYAPQSQLQLSPGMDMDIKRVLHYSQSLAAMGGSPNGPAGQGVNGSSGMGHHMSSHMTPHHMHQAVSAQQTLSPNSSIGSAGSLGSQSSLGSNGSGLNSSSGHQSAGMHSLATSPGQEGHIKRPMNAFMVWSRLQRRQIAKDNPKMHNSEISKRLGAEWKLLAESEKRPFIDEAKRLRALHMKEHPDYKYRPRRKPKNPLTAGPQGGLQMQAGGMGQQKLGAGPGAGAGGYNPFHQLPPYFAPSHHLDQGYPVPYFGGFDPLALSKLHQSQAAAAAAVNNQGQQQGQAPPQLPPTSLSSFYSGIYSGISAPSLYAAHSANAAGLYPSSSTSSPGSSPGTITPNGMDGSMDSALRRPVPVLY

Target ORF information:

RefSeq Version	NM_001274902.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dichaete, transcript variant C (D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274902.1

ORF Insert Sequence:

ATGGCCACCT TATCGACACA CCCCAATTAC GGTTTCCATT TGGGTCAGGC CCAAGGCTTG 
GAGGACTACG CACCGCAAAG TCAGCTCCAG CTGAGTCCCG GCATGGACAT GGATATCAAG 
CGTGTGCTGC ACTACTCCCA AAGCCTGGCA GCCATGGGCG GATCGCCCAA TGGACCCGCC 
GGCCAGGGTG TGAACGGATC CTCGGGCATG GGTCACCACA TGTCCAGTCA CATGACCCCG 
CACCACATGC ACCAGGCGGT CTCCGCCCAA CAGACCTTGT CGCCCAACAG TTCCATCGGA 
TCCGCCGGCA GTCTGGGTAG CCAGAGCAGC CTGGGCAGCA ATGGCAGTGG TCTCAACTCC 
AGTTCGGGTC ACCAGTCCGC CGGCATGCAC AGTTTGGCCA CATCGCCGGG ACAAGAGGGT 
CACATCAAGC GTCCAATGAA CGCGTTCATG GTCTGGTCCC GCCTGCAGCG TCGTCAGATC 
GCCAAGGATA ACCCCAAGAT GCACAACTCT GAGATCTCCA AGCGCCTGGG TGCCGAGTGG 
AAGTTGCTGG CCGAGTCGGA GAAACGACCA TTCATCGATG AGGCCAAGCG CTTGAGGGCC 
CTGCACATGA AGGAGCACCC GGACTACAAG TACCGGCCAC GCCGCAAGCC CAAGAACCCA 
CTGACCGCTG GACCCCAGGG TGGACTGCAG ATGCAGGCCG GCGGCATGGG TCAACAGAAG 
TTGGGAGCTG GACCGGGAGC TGGAGCCGGA GGCTACAATC CATTCCATCA ACTACCGCCA 
TACTTTGCAC CCTCACACCA TCTGGATCAG GGTTATCCAG TGCCGTACTT CGGTGGATTT 
GACCCACTGG CTCTATCGAA ATTGCATCAA TCCCAGGCAG CGGCGGCGGC GGCGGTCAAC 
AATCAGGGTC AGCAGCAAGG ACAAGCCCCG CCCCAATTGC CGCCCACCTC GCTGAGCAGC 
TTCTACTCGG GCATTTATTC CGGCATCTCG GCACCTTCGC TGTACGCCGC ACACTCCGCC 
AATGCCGCCG GACTCTACCC ATCCTCGTCC ACCTCCTCGC CGGGATCTTC GCCCGGAACC 
ATCACGCCAA ATGGTATGGA TGGTTCCATG GACAGTGCCC TGAGGCGACC GGTTCCGGTG 
CTCTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

D ( NM_001274903.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261832.1 Drosophila melanogaster dichaete, transcript variant D (D), mRNA.

Price & Availability↑

CloneID	OFa18484
Clone ID Related Accession (Same CDS sequence)	NM_079342.3 , NM_001274902.1 , NM_001274903.1 , NM_001274901.1
Accession Version	NM_001274903.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dichaete, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCACCT TATCGACACA CCCCAATTAC GGTTTCCATT TGGGTCAGGC CCAAGGCTTG 
GAGGACTACG CACCGCAAAG TCAGCTCCAG CTGAGTCCCG GCATGGACAT GGATATCAAG 
CGTGTGCTGC ACTACTCCCA AAGCCTGGCA GCCATGGGCG GATCGCCCAA TGGACCCGCC 
GGCCAGGGTG TGAACGGATC CTCGGGCATG GGTCACCACA TGTCCAGTCA CATGACCCCG 
CACCACATGC ACCAGGCGGT CTCCGCCCAA CAGACCTTGT CGCCCAACAG TTCCATCGGA 
TCCGCCGGCA GTCTGGGTAG CCAGAGCAGC CTGGGCAGCA ATGGCAGTGG TCTCAACTCC 
AGTTCGGGTC ACCAGTCCGC CGGCATGCAC AGTTTGGCCA CATCGCCGGG ACAAGAGGGT 
CACATCAAGC GTCCAATGAA CGCGTTCATG GTCTGGTCCC GCCTGCAGCG TCGTCAGATC 
GCCAAGGATA ACCCCAAGAT GCACAACTCT GAGATCTCCA AGCGCCTGGG TGCCGAGTGG 
AAGTTGCTGG CCGAGTCGGA GAAACGACCA TTCATCGATG AGGCCAAGCG CTTGAGGGCC 
CTGCACATGA AGGAGCACCC GGACTACAAG TACCGGCCAC GCCGCAAGCC CAAGAACCCA 
CTGACCGCTG GACCCCAGGG TGGACTGCAG ATGCAGGCCG GCGGCATGGG TCAACAGAAG 
TTGGGAGCTG GACCGGGAGC TGGAGCCGGA GGCTACAATC CATTCCATCA ACTACCGCCA 
TACTTTGCAC CCTCACACCA TCTGGATCAG GGTTATCCAG TGCCGTACTT CGGTGGATTT 
GACCCACTGG CTCTATCGAA ATTGCATCAA TCCCAGGCAG CGGCGGCGGC GGCGGTCAAC 
AATCAGGGTC AGCAGCAAGG ACAAGCCCCG CCCCAATTGC CGCCCACCTC GCTGAGCAGC 
TTCTACTCGG GCATTTATTC CGGCATCTCG GCACCTTCGC TGTACGCCGC ACACTCCGCC 
AATGCCGCCG GACTCTACCC ATCCTCGTCC ACCTCCTCGC CGGGATCTTC GCCCGGAACC 
ATCACGCCAA ATGGTATGGA TGGTTCCATG GACAGTGCCC TGAGGCGACC GGTTCCGGTG 
CTCTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261832.1
CDS	1207..2355
Translation	MATLSTHPNYGFHLGQAQGLEDYAPQSQLQLSPGMDMDIKRVLHYSQSLAAMGGSPNGPAGQGVNGSSGMGHHMSSHMTPHHMHQAVSAQQTLSPNSSIGSAGSLGSQSSLGSNGSGLNSSSGHQSAGMHSLATSPGQEGHIKRPMNAFMVWSRLQRRQIAKDNPKMHNSEISKRLGAEWKLLAESEKRPFIDEAKRLRALHMKEHPDYKYRPRRKPKNPLTAGPQGGLQMQAGGMGQQKLGAGPGAGAGGYNPFHQLPPYFAPSHHLDQGYPVPYFGGFDPLALSKLHQSQAAAAAAVNNQGQQQGQAPPQLPPTSLSSFYSGIYSGISAPSLYAAHSANAAGLYPSSSTSSPGSSPGTITPNGMDGSMDSALRRPVPVLY

Target ORF information:

RefSeq Version	NM_001274903.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dichaete, transcript variant D (D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274903.1

ORF Insert Sequence:

ATGGCCACCT TATCGACACA CCCCAATTAC GGTTTCCATT TGGGTCAGGC CCAAGGCTTG 
GAGGACTACG CACCGCAAAG TCAGCTCCAG CTGAGTCCCG GCATGGACAT GGATATCAAG 
CGTGTGCTGC ACTACTCCCA AAGCCTGGCA GCCATGGGCG GATCGCCCAA TGGACCCGCC 
GGCCAGGGTG TGAACGGATC CTCGGGCATG GGTCACCACA TGTCCAGTCA CATGACCCCG 
CACCACATGC ACCAGGCGGT CTCCGCCCAA CAGACCTTGT CGCCCAACAG TTCCATCGGA 
TCCGCCGGCA GTCTGGGTAG CCAGAGCAGC CTGGGCAGCA ATGGCAGTGG TCTCAACTCC 
AGTTCGGGTC ACCAGTCCGC CGGCATGCAC AGTTTGGCCA CATCGCCGGG ACAAGAGGGT 
CACATCAAGC GTCCAATGAA CGCGTTCATG GTCTGGTCCC GCCTGCAGCG TCGTCAGATC 
GCCAAGGATA ACCCCAAGAT GCACAACTCT GAGATCTCCA AGCGCCTGGG TGCCGAGTGG 
AAGTTGCTGG CCGAGTCGGA GAAACGACCA TTCATCGATG AGGCCAAGCG CTTGAGGGCC 
CTGCACATGA AGGAGCACCC GGACTACAAG TACCGGCCAC GCCGCAAGCC CAAGAACCCA 
CTGACCGCTG GACCCCAGGG TGGACTGCAG ATGCAGGCCG GCGGCATGGG TCAACAGAAG 
TTGGGAGCTG GACCGGGAGC TGGAGCCGGA GGCTACAATC CATTCCATCA ACTACCGCCA 
TACTTTGCAC CCTCACACCA TCTGGATCAG GGTTATCCAG TGCCGTACTT CGGTGGATTT 
GACCCACTGG CTCTATCGAA ATTGCATCAA TCCCAGGCAG CGGCGGCGGC GGCGGTCAAC 
AATCAGGGTC AGCAGCAAGG ACAAGCCCCG CCCCAATTGC CGCCCACCTC GCTGAGCAGC 
TTCTACTCGG GCATTTATTC CGGCATCTCG GCACCTTCGC TGTACGCCGC ACACTCCGCC 
AATGCCGCCG GACTCTACCC ATCCTCGTCC ACCTCCTCGC CGGGATCTTC GCCCGGAACC 
ATCACGCCAA ATGGTATGGA TGGTTCCATG GACAGTGCCC TGAGGCGACC GGTTCCGGTG 
CTCTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

D ( NM_001274901.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261830.1 Drosophila melanogaster dichaete, transcript variant B (D), mRNA.

Price & Availability↑

CloneID	OFa18484
Clone ID Related Accession (Same CDS sequence)	NM_079342.3 , NM_001274902.1 , NM_001274903.1 , NM_001274901.1
Accession Version	NM_001274901.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dichaete, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCACCT TATCGACACA CCCCAATTAC GGTTTCCATT TGGGTCAGGC CCAAGGCTTG 
GAGGACTACG CACCGCAAAG TCAGCTCCAG CTGAGTCCCG GCATGGACAT GGATATCAAG 
CGTGTGCTGC ACTACTCCCA AAGCCTGGCA GCCATGGGCG GATCGCCCAA TGGACCCGCC 
GGCCAGGGTG TGAACGGATC CTCGGGCATG GGTCACCACA TGTCCAGTCA CATGACCCCG 
CACCACATGC ACCAGGCGGT CTCCGCCCAA CAGACCTTGT CGCCCAACAG TTCCATCGGA 
TCCGCCGGCA GTCTGGGTAG CCAGAGCAGC CTGGGCAGCA ATGGCAGTGG TCTCAACTCC 
AGTTCGGGTC ACCAGTCCGC CGGCATGCAC AGTTTGGCCA CATCGCCGGG ACAAGAGGGT 
CACATCAAGC GTCCAATGAA CGCGTTCATG GTCTGGTCCC GCCTGCAGCG TCGTCAGATC 
GCCAAGGATA ACCCCAAGAT GCACAACTCT GAGATCTCCA AGCGCCTGGG TGCCGAGTGG 
AAGTTGCTGG CCGAGTCGGA GAAACGACCA TTCATCGATG AGGCCAAGCG CTTGAGGGCC 
CTGCACATGA AGGAGCACCC GGACTACAAG TACCGGCCAC GCCGCAAGCC CAAGAACCCA 
CTGACCGCTG GACCCCAGGG TGGACTGCAG ATGCAGGCCG GCGGCATGGG TCAACAGAAG 
TTGGGAGCTG GACCGGGAGC TGGAGCCGGA GGCTACAATC CATTCCATCA ACTACCGCCA 
TACTTTGCAC CCTCACACCA TCTGGATCAG GGTTATCCAG TGCCGTACTT CGGTGGATTT 
GACCCACTGG CTCTATCGAA ATTGCATCAA TCCCAGGCAG CGGCGGCGGC GGCGGTCAAC 
AATCAGGGTC AGCAGCAAGG ACAAGCCCCG CCCCAATTGC CGCCCACCTC GCTGAGCAGC 
TTCTACTCGG GCATTTATTC CGGCATCTCG GCACCTTCGC TGTACGCCGC ACACTCCGCC 
AATGCCGCCG GACTCTACCC ATCCTCGTCC ACCTCCTCGC CGGGATCTTC GCCCGGAACC 
ATCACGCCAA ATGGTATGGA TGGTTCCATG GACAGTGCCC TGAGGCGACC GGTTCCGGTG 
CTCTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261830.1
CDS	200..1348
Translation	MATLSTHPNYGFHLGQAQGLEDYAPQSQLQLSPGMDMDIKRVLHYSQSLAAMGGSPNGPAGQGVNGSSGMGHHMSSHMTPHHMHQAVSAQQTLSPNSSIGSAGSLGSQSSLGSNGSGLNSSSGHQSAGMHSLATSPGQEGHIKRPMNAFMVWSRLQRRQIAKDNPKMHNSEISKRLGAEWKLLAESEKRPFIDEAKRLRALHMKEHPDYKYRPRRKPKNPLTAGPQGGLQMQAGGMGQQKLGAGPGAGAGGYNPFHQLPPYFAPSHHLDQGYPVPYFGGFDPLALSKLHQSQAAAAAAVNNQGQQQGQAPPQLPPTSLSSFYSGIYSGISAPSLYAAHSANAAGLYPSSSTSSPGSSPGTITPNGMDGSMDSALRRPVPVLY

Target ORF information:

RefSeq Version	NM_001274901.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dichaete, transcript variant B (D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274901.1

ORF Insert Sequence:

ATGGCCACCT TATCGACACA CCCCAATTAC GGTTTCCATT TGGGTCAGGC CCAAGGCTTG 
GAGGACTACG CACCGCAAAG TCAGCTCCAG CTGAGTCCCG GCATGGACAT GGATATCAAG 
CGTGTGCTGC ACTACTCCCA AAGCCTGGCA GCCATGGGCG GATCGCCCAA TGGACCCGCC 
GGCCAGGGTG TGAACGGATC CTCGGGCATG GGTCACCACA TGTCCAGTCA CATGACCCCG 
CACCACATGC ACCAGGCGGT CTCCGCCCAA CAGACCTTGT CGCCCAACAG TTCCATCGGA 
TCCGCCGGCA GTCTGGGTAG CCAGAGCAGC CTGGGCAGCA ATGGCAGTGG TCTCAACTCC 
AGTTCGGGTC ACCAGTCCGC CGGCATGCAC AGTTTGGCCA CATCGCCGGG ACAAGAGGGT 
CACATCAAGC GTCCAATGAA CGCGTTCATG GTCTGGTCCC GCCTGCAGCG TCGTCAGATC 
GCCAAGGATA ACCCCAAGAT GCACAACTCT GAGATCTCCA AGCGCCTGGG TGCCGAGTGG 
AAGTTGCTGG CCGAGTCGGA GAAACGACCA TTCATCGATG AGGCCAAGCG CTTGAGGGCC 
CTGCACATGA AGGAGCACCC GGACTACAAG TACCGGCCAC GCCGCAAGCC CAAGAACCCA 
CTGACCGCTG GACCCCAGGG TGGACTGCAG ATGCAGGCCG GCGGCATGGG TCAACAGAAG 
TTGGGAGCTG GACCGGGAGC TGGAGCCGGA GGCTACAATC CATTCCATCA ACTACCGCCA 
TACTTTGCAC CCTCACACCA TCTGGATCAG GGTTATCCAG TGCCGTACTT CGGTGGATTT 
GACCCACTGG CTCTATCGAA ATTGCATCAA TCCCAGGCAG CGGCGGCGGC GGCGGTCAAC 
AATCAGGGTC AGCAGCAAGG ACAAGCCCCG CCCCAATTGC CGCCCACCTC GCTGAGCAGC 
TTCTACTCGG GCATTTATTC CGGCATCTCG GCACCTTCGC TGTACGCCGC ACACTCCGCC 
AATGCCGCCG GACTCTACCC ATCCTCGTCC ACCTCCTCGC CGGGATCTTC GCCCGGAACC 
ATCACGCCAA ATGGTATGGA TGGTTCCATG GACAGTGCCC TGAGGCGACC GGTTCCGGTG 
CTCTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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D cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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