JIL-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	JIL-1
Entrez Gene ID	39241
Full Name	JIL-1 kinase
Synonyms	2Ab17,CG6297,Dmel\CG6297,IL-1,JIL,JIL1,Jil-1,Jil1,MSK,MSK1,Su(var)3-1,Su(var)3-103,Su-var(3)1,Su-var(3)103,anon-WO0118547.379,mAb2A
General protein information	Preferred Names JIL-1 kinase Names CG6297 gene product from transcript CG6297-RC CG6297-PA CG6297-PB CG6297-PC JIL-1 histone H3S10 kinase JIL-1 histone kinase JIL-1-PA JIL-1-PB JIL-1-PC suppressor of variegation 3-1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 68A5-68A6\|3-31.4 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_140175.3	NP_648432.1	JIL-1 kinase, isoform B
NM_001274768.1	NP_001261697.1	JIL-1 kinase, isoform C
NM_168439.2	NP_729661.1	JIL-1 kinase, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	RPS6KA5	NP_004746.2
Bos taurus (cattle)	RPS6KA5	NP_001178952.1
Drosophila melanogaster (fruit fly)	JIL-1	NP_001261697.1
Pan troglodytes (chimpanzee)	RPS6KA5	XP_001140389.2
	RPS6KA5	XP_001088246.2
Canis lupus familiaris (dog)	RPS6KA5	XP_005623800.1
Mus musculus (house mouse)	Rps6ka5	NP_705815.1
Gallus gallus (chicken)	RPS6KA5	NP_001012605.1
Danio rerio (zebrafish)	LOC565173	XP_693550.4
Caenorhabditis elegans (roundworm)	rskn-2	NP_001040645.1
Xenopus tropicalis (tropical clawed frog)	rps6ka5	XP_002933270.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

our data indicate that the conserved H3S28 residue in metazoans has a role in supporting PRC2 catalysis.
Title: Histone H3 Serine 28 Is Essential for Efficient Polycomb-Mediated Gene Repression in Drosophila.

JIL-1 regulated heterochromatin in the context of the chromocenter and fourth chromosome by creating a composite H3S10phK9me2 mark together with the Su(var)3-9 methyltransferase.
Title: Histone H3S10 phosphorylation by the JIL-1 kinase in pericentric heterochromatin and on the fourth chromosome creates a composite H3S10phK9me2 epigenetic mark.

Enrichment of JIL-1 and the H3S10ph mark are predominantly associated with active genes in Drosophila.
Title: Genome-wide analysis of regulation of gene expression and H3K9me2 distribution by JIL-1 kinase mediated histone H3S10 phosphorylation in Drosophila.

epigenetic H3S10 phosphorylation mark itself functions as a causative regulator of chromatin structure independently of any structural contributions from the JIL-1 protein.
Title: Domain requirements of the JIL-1 tandem kinase for histone H3 serine 10 phosphorylation and chromatin remodeling in vivo.

results strongly suggest that the action of JIL-1 depends on which telomere domain, which chromosome and which promoter is embedded in the telomere chromatin.
Title: The JIL-1 kinase affects telomere expression in the different telomere domains of Drosophila.

The following JIL-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the JIL-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa18204	NM_168439.2 Latest version!	Drosophila melanogaster JIL-1 kinase (JIL-1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa18204	NM_140175.3 Latest version!	Drosophila melanogaster JIL-1 kinase (JIL-1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa18204	NM_001274768.1 Latest version!	Drosophila melanogaster JIL-1 kinase (JIL-1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

JIL-1 ( NM_140175.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648432.1 Drosophila melanogaster JIL-1 kinase (JIL-1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa18204
Clone ID Related Accession (Same CDS sequence)	NM_140175.3 , NM_001274768.1 , NM_168439.2
Accession Version	NM_140175.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	JIL-1 kinase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140175.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTCGCT TGCAAAAGCA AAACTACGAG ATCTTAAGCG GTACAAGTAC GTCGCGCCTC 
AAGAATCATC AACATCCCCG TGAATCGGAG TCACTAGCCT ACGAGGAGCC AGATCAAATG 
GTGCGGAATC ATCTAAATGG CCAGCTGGTA GCCAATGGCA ATGGCAAAAC GCGAAAGAAT 
AGCAATTCCG AAACGATGAC CAATGGGAAA AAGTCCAAAC TGAATACGGA GGGATCCGGC 
TCTGGTTCTG GAAAGACCTT GAACTACAAT AATAATAACA ATAACAACAA CAGCATCAGT 
GCCACCAACG GTCAGTACAC CAATAGCAGC AGCAAGACCA CTTCAGCATC TGCAAGGGAT 
TACACTTACC GCGAGACCAT ATCACCGCCC ACACCTCCAT CCCCGCCCAC CACAAATGTG 
GCGGATATTG TTTGCATATC GGATGCGGAG AGCGAGGACG GACGCGATCC CGAGCGGGAG 
TACTACGACC AGGACATGGA GGAGGATGAG CCGAACGGGA TAGAGATCGA TGAATCCAGC 
TCCAGCTTGT CCAAGGCCAA GTCCAACAAT GCAGCTGCAG CGGCGGCGGC GGCGGCTGCA 
GCAGCGGCAG CAGCAGCCTC GAAGGCCTCC TCGTCGACCA CGCCCTCCTA CGCCATGCCC 
ACCAGCAATA GTACACCCCT TGATCTGGAC AATGAGGCCC ATCAACGGGA CCTGGAGGCG 
GTCACCGATC TGAAATACTA CGTAAAATTG TACAGTGATG AGGCCGTTAG TCTGAATGAC 
TTCAAAATAA TACGCGTTCT CGGAACAGGT GCCTATGGGC GGGTTTTCCT CGTTCGCAAG 
CTAACTAGAC ACGATGCCGG AAAATTGTAT GCAATGAAGG TTCTCAATAA GATAACGGTG 
GTCCAGAAGC GAAAAACCGC GGAACACACC AAAACGGAGA GAGTGGTACT GGAGGCCATT 
CAACGTAATC CCTTCCTCGT GAGTCTACAC TACGCCTTCC AATCCTCATC AAAGCTTTAC 
CTCGTGCTAG ACTTTGCCAA TGGCGGTGAG CTCTTCACGC ATCTCTACCA CTCTGAGAAC 
TTTGAGGAGT CCCGGGTGCG TGTGTACATA GCCGAGGTGG TGCTGGCCCT GGAGCAACTG 
CATCAGTTGG GGATCATCTA TCGAGATATT AAGTTGGAGA ATATTTTGCT TGATGGCGAG 
GGGCACATTG TTCTGTCCGA CTTTGGACTG TCGAAGATCC TTACCGCGGA GAATGAATAC 
CGTGCGCATA GCTTCTGCGG CACTCTGGAG TATATGGCAC CTGAGATAAT CCGTACGGGG 
CCGCCAGGAC ACGACAGTGC CGTGGACTGG TGGTCGGTGG GTGTGCTCAC TTTTGAGCTG 
CTCACCGGCG CTTCGCCATT CGCCACGTCC GACGGCCAGG TCCAGCAATC GGAAATCTCG 
CGGCGGATTC AGAAGGAGCA GCCAATGATT CCCTCATCGT TCAGTGCCAA CGCCCGCGAC 
TTTGTGCTAA AGATGTTGGA AAAGAATCCA AAGCGGCGCT TGGGTGGCAA CCATCGCGAC 
GCCAGCGAAA TCAAGGAGCA CCCCTTCTTC AACGGCATTA ACTGGCAGGA GTTGCGGACA 
AAGCGCCGCA AAGCCCCCTA TAAGCCCACC CTCACCGCCG AGGACGATGT GCAGAACTTC 
AGCAACGAGT TTACCGACCA AGTGCCCGAG GATCCCGAGT GCGATGCTCC GCCCAGCCGG 
ATACGACTAT TTCGCGGTTA CACATACGTG GCGCCAGAGC ATTTGGAGCA GATGCGAAGG 
GATAACCACT GTGAGATTCA ATACTTTAAT ACAGGACTTC AGAATATACC TTGTCGGCCA 
GATGATCTTG AATTGGGCAC ACGCACTTCA AACGGAGCCT ATGGCACATG CCATTTTGTG 
GTGGACAGCT CTACGGATTT GGTGTTTTTG GCCAAAATAA TACCGCTATC CAAGTTCCGC 
CCCTCCGAGG TCGACGCGCT GATCTCGTGC GCTCTCGACA CGACCAATCA CAAAAATATC 
GTAAGCTACC ATGGAACGTT CAGGGAAAAG TGCGAGACAT GGATCGTTAT GGAGTACCTG 
TCCGGCCCGG AGCTGACCGC ATCCATTCGC ATGGATGAGG ATTCGTGTCG GGAGATCTTT 
CTGCAGCTGG TCATGGCTGT GCGGCACATA CACTCCAAGC ACTTTATACA CGGTGACCTG 
AAACCGGAGA ACATAATGTT CGAGAACCGA GAGGATAGGA CTGTCAAACT GATCGATTTT 
GGCAGCGCAT GTTACAATAA TCGCTTTAAG AGCTGGAAGG ACAAGCCGCG CTACACGCTG 
GACTATGCTC CGCCAGAAAT GCTCGCGGAT GCGAATCTCG TTACTTATTC GCCAGCCGTG 
GATATATACG GTCTTGGGGC CACGCTGTAT ACCATGCTGG TGGGTCATCG GCCGTACAGA 
CAGAACGAAG ACGATGTGGA TCATTCCGCA GCGGCCCATC ACGAGCTTCG AAAGCGAATG 
CGCCGGGGAA CGTTTAATCA GCGTTCTATG CGCTGGGAGA GTGCAAGTCC TGCGTTCCGA 
CATTTGGTTA GCTGGTGTCT GCAGCGGGAT CCGGCTGATC GGCCCACATT GTCAGATATT 
CTCGACAGCG AGTGGCTGCA GTACGGCTCA AATGATCCGG ACGTGGATAT CATTCTGCCA 
CAACAGATGG TTGTGGACCT CAGCGAAGAC ACTATGGAAC AACCGACGGG AGGTATGTTC 
GACGATCAGC AACAACTGGA ATTCATGCAC GACAAGTCGG CGGAGGATGA GGGCATAACC 
CTAGTCAGCG AGCCCATGGA TACGACTGTC GCGACTCACG AGTCTAGGAG AAATGCAGCC 
GCATTTTCGT CGGTAGTTGC TCCAACCACC GACGACGAGA TAGTGCACGA ACGATTTGAC 
CCGGCATTTG AGGTCCAAGC GGACTTCTAC GGTTTCGACG AGAATGCGCC GCCTCTGCCT 
CTGCCCGAGG AGTACTACTC CGAACTACCT CTGCCAGAGG AGGACCGGCA GTACATACCA 
CCTCCACCAG CACTGATTCC TGTTGAACCA GAAACGACCT TCCGACGGCC AAGAACACGC 
CAGCAGCGTC GCACTGAAAG CCAGCTAGTA CAACCTGTTT CCGTCGCTAC GTATGAGGAC 
AGCAAGGCGT CCCTGCGCGT ACTAATGCAA CAGCTGCCGC CGCCAGGTGA CAATGTGGTA 
GCACGCATTC CGAAGAGAAC ACATCGAGTC GTACGTACAT TGCCTCCGAC CTTTGGAACC 
ACCAAGCGAG AAGAGAATTT CTATGGATTT AGTAAGACAG CAATATCGTG GCGAAAGACT 
CGGGCCAGCT GGCGTCACTT CTGTCTGCTT ATCAATGGTG TGCAGCAGGT GCTTAAGGTT 
CGGTTCAAAA AAGCGCGTCG CGTCTACTGC CTGCCCCATA TAAAAGAGGA AAAACTTGAT 
CATGCGTACG AAAAACCGTT GACGTTCCCG CGGCCAAAGG CACAACTGAA GCGCACCAAG 
CGGGAGCCCA AGGTGCCGCG GCCACCGACT CGAGTTCAGC CGGAGAGGGC GCGGGCAATG 
CGTCAACTTT ATCAGTTCCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648432.1
CDS	1056..4679
Translation	MSRLQKQNYEILSGTSTSRLKNHQHPRESESLAYEEPDQMVRNHLNGQLVANGNGKTRKNSNSETMTNGKKSKLNTEGSGSGSGKTLNYNNNNNNNNSISATNGQYTNSSSKTTSASARDYTYRETISPPTPPSPPTTNVADIVCISDAESEDGRDPEREYYDQDMEEDEPNGIEIDESSSSLSKAKSNNAAAAAAAAAAAAAAAASKASSSTTPSYAMPTSNSTPLDLDNEAHQRDLEAVTDLKYYVKLYSDEAVSLNDFKIIRVLGTGAYGRVFLVRKLTRHDAGKLYAMKVLNKITVVQKRKTAEHTKTERVVLEAIQRNPFLVSLHYAFQSSSKLYLVLDFANGGELFTHLYHSENFEESRVRVYIAEVVLALEQLHQLGIIYRDIKLENILLDGEGHIVLSDFGLSKILTAENEYRAHSFCGTLEYMAPEIIRTGPPGHDSAVDWWSVGVLTFELLTGASPFATSDGQVQQSEISRRIQKEQPMIPSSFSANARDFVLKMLEKNPKRRLGGNHRDASEIKEHPFFNGINWQELRTKRRKAPYKPTLTAEDDVQNFSNEFTDQVPEDPECDAPPSRIRLFRGYTYVAPEHLEQMRRDNHCEIQYFNTGLQNIPCRPDDLELGTRTSNGAYGTCHFVVDSSTDLVFLAKIIPLSKFRPSEVDALISCALDTTNHKNIVSYHGTFREKCETWIVMEYLSGPELTASIRMDEDSCREIFLQLVMAVRHIHSKHFIHGDLKPENIMFENREDRTVKLIDFGSACYNNRFKSWKDKPRYTLDYAPPEMLADANLVTYSPAVDIYGLGATLYTMLVGHRPYRQNEDDVDHSAAAHHELRKRMRRGTFNQRSMRWESASPAFRHLVSWCLQRDPADRPTLSDILDSEWLQYGSNDPDVDIILPQQMVVDLSEDTMEQPTGGMFDDQQQLEFMHDKSAEDEGITLVSEPMDTTVATHESRRNAAAFSSVVAPTTDDEIVHERFDPAFEVQADFYGFDENAPPLPLPEEYYSELPLPEEDRQYIPPPPALIPVEPETTFRRPRTRQQRRTESQLVQPVSVATYEDSKASLRVLMQQLPPPGDNVVARIPKRTHRVVRTLPPTFGTTKREENFYGFSKTAISWRKTRASWRHFCLLINGVQQVLKVRFKKARRVYCLPHIKEEKLDHAYEKPLTFPRPKAQLKRTKREPKVPRPPTRVQPERARAMRQLYQFQ

Target ORF information:

RefSeq Version	NM_140175.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster JIL-1 kinase (JIL-1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140175.3

ORF Insert Sequence:

ATGAGTCGCT TGCAAAAGCA AAACTACGAG ATCTTAAGCG GTACAAGTAC GTCGCGCCTC 
AAGAATCATC AACATCCCCG TGAATCGGAG TCACTAGCCT ACGAGGAGCC AGATCAAATG 
GTGCGGAATC ATCTAAATGG CCAGCTGGTA GCCAATGGCA ATGGCAAAAC GCGAAAGAAT 
AGCAATTCCG AAACGATGAC CAATGGGAAA AAGTCCAAAC TGAATACGGA GGGATCCGGC 
TCTGGTTCTG GAAAGACCTT GAACTACAAT AATAATAACA ATAACAACAA CAGCATCAGT 
GCCACCAACG GTCAGTACAC CAATAGCAGC AGCAAGACCA CTTCAGCATC TGCAAGGGAT 
TACACTTACC GCGAGACCAT ATCACCGCCC ACACCTCCAT CCCCGCCCAC CACAAATGTG 
GCGGATATTG TTTGCATATC GGATGCGGAG AGCGAGGACG GACGCGATCC CGAGCGGGAG 
TACTACGACC AGGACATGGA GGAGGATGAG CCGAACGGGA TAGAGATCGA TGAATCCAGC 
TCCAGCTTGT CCAAGGCCAA GTCCAACAAT GCAGCTGCAG CGGCGGCGGC GGCGGCTGCA 
GCAGCGGCAG CAGCAGCCTC GAAGGCCTCC TCGTCGACCA CGCCCTCCTA CGCCATGCCC 
ACCAGCAATA GTACACCCCT TGATCTGGAC AATGAGGCCC ATCAACGGGA CCTGGAGGCG 
GTCACCGATC TGAAATACTA CGTAAAATTG TACAGTGATG AGGCCGTTAG TCTGAATGAC 
TTCAAAATAA TACGCGTTCT CGGAACAGGT GCCTATGGGC GGGTTTTCCT CGTTCGCAAG 
CTAACTAGAC ACGATGCCGG AAAATTGTAT GCAATGAAGG TTCTCAATAA GATAACGGTG 
GTCCAGAAGC GAAAAACCGC GGAACACACC AAAACGGAGA GAGTGGTACT GGAGGCCATT 
CAACGTAATC CCTTCCTCGT GAGTCTACAC TACGCCTTCC AATCCTCATC AAAGCTTTAC 
CTCGTGCTAG ACTTTGCCAA TGGCGGTGAG CTCTTCACGC ATCTCTACCA CTCTGAGAAC 
TTTGAGGAGT CCCGGGTGCG TGTGTACATA GCCGAGGTGG TGCTGGCCCT GGAGCAACTG 
CATCAGTTGG GGATCATCTA TCGAGATATT AAGTTGGAGA ATATTTTGCT TGATGGCGAG 
GGGCACATTG TTCTGTCCGA CTTTGGACTG TCGAAGATCC TTACCGCGGA GAATGAATAC 
CGTGCGCATA GCTTCTGCGG CACTCTGGAG TATATGGCAC CTGAGATAAT CCGTACGGGG 
CCGCCAGGAC ACGACAGTGC CGTGGACTGG TGGTCGGTGG GTGTGCTCAC TTTTGAGCTG 
CTCACCGGCG CTTCGCCATT CGCCACGTCC GACGGCCAGG TCCAGCAATC GGAAATCTCG 
CGGCGGATTC AGAAGGAGCA GCCAATGATT CCCTCATCGT TCAGTGCCAA CGCCCGCGAC 
TTTGTGCTAA AGATGTTGGA AAAGAATCCA AAGCGGCGCT TGGGTGGCAA CCATCGCGAC 
GCCAGCGAAA TCAAGGAGCA CCCCTTCTTC AACGGCATTA ACTGGCAGGA GTTGCGGACA 
AAGCGCCGCA AAGCCCCCTA TAAGCCCACC CTCACCGCCG AGGACGATGT GCAGAACTTC 
AGCAACGAGT TTACCGACCA AGTGCCCGAG GATCCCGAGT GCGATGCTCC GCCCAGCCGG 
ATACGACTAT TTCGCGGTTA CACATACGTG GCGCCAGAGC ATTTGGAGCA GATGCGAAGG 
GATAACCACT GTGAGATTCA ATACTTTAAT ACAGGACTTC AGAATATACC TTGTCGGCCA 
GATGATCTTG AATTGGGCAC ACGCACTTCA AACGGAGCCT ATGGCACATG CCATTTTGTG 
GTGGACAGCT CTACGGATTT GGTGTTTTTG GCCAAAATAA TACCGCTATC CAAGTTCCGC 
CCCTCCGAGG TCGACGCGCT GATCTCGTGC GCTCTCGACA CGACCAATCA CAAAAATATC 
GTAAGCTACC ATGGAACGTT CAGGGAAAAG TGCGAGACAT GGATCGTTAT GGAGTACCTG 
TCCGGCCCGG AGCTGACCGC ATCCATTCGC ATGGATGAGG ATTCGTGTCG GGAGATCTTT 
CTGCAGCTGG TCATGGCTGT GCGGCACATA CACTCCAAGC ACTTTATACA CGGTGACCTG 
AAACCGGAGA ACATAATGTT CGAGAACCGA GAGGATAGGA CTGTCAAACT GATCGATTTT 
GGCAGCGCAT GTTACAATAA TCGCTTTAAG AGCTGGAAGG ACAAGCCGCG CTACACGCTG 
GACTATGCTC CGCCAGAAAT GCTCGCGGAT GCGAATCTCG TTACTTATTC GCCAGCCGTG 
GATATATACG GTCTTGGGGC CACGCTGTAT ACCATGCTGG TGGGTCATCG GCCGTACAGA 
CAGAACGAAG ACGATGTGGA TCATTCCGCA GCGGCCCATC ACGAGCTTCG AAAGCGAATG 
CGCCGGGGAA CGTTTAATCA GCGTTCTATG CGCTGGGAGA GTGCAAGTCC TGCGTTCCGA 
CATTTGGTTA GCTGGTGTCT GCAGCGGGAT CCGGCTGATC GGCCCACATT GTCAGATATT 
CTCGACAGCG AGTGGCTGCA GTACGGCTCA AATGATCCGG ACGTGGATAT CATTCTGCCA 
CAACAGATGG TTGTGGACCT CAGCGAAGAC ACTATGGAAC AACCGACGGG AGGTATGTTC 
GACGATCAGC AACAACTGGA ATTCATGCAC GACAAGTCGG CGGAGGATGA GGGCATAACC 
CTAGTCAGCG AGCCCATGGA TACGACTGTC GCGACTCACG AGTCTAGGAG AAATGCAGCC 
GCATTTTCGT CGGTAGTTGC TCCAACCACC GACGACGAGA TAGTGCACGA ACGATTTGAC 
CCGGCATTTG AGGTCCAAGC GGACTTCTAC GGTTTCGACG AGAATGCGCC GCCTCTGCCT 
CTGCCCGAGG AGTACTACTC CGAACTACCT CTGCCAGAGG AGGACCGGCA GTACATACCA 
CCTCCACCAG CACTGATTCC TGTTGAACCA GAAACGACCT TCCGACGGCC AAGAACACGC 
CAGCAGCGTC GCACTGAAAG CCAGCTAGTA CAACCTGTTT CCGTCGCTAC GTATGAGGAC 
AGCAAGGCGT CCCTGCGCGT ACTAATGCAA CAGCTGCCGC CGCCAGGTGA CAATGTGGTA 
GCACGCATTC CGAAGAGAAC ACATCGAGTC GTACGTACAT TGCCTCCGAC CTTTGGAACC 
ACCAAGCGAG AAGAGAATTT CTATGGATTT AGTAAGACAG CAATATCGTG GCGAAAGACT 
CGGGCCAGCT GGCGTCACTT CTGTCTGCTT ATCAATGGTG TGCAGCAGGT GCTTAAGGTT 
CGGTTCAAAA AAGCGCGTCG CGTCTACTGC CTGCCCCATA TAAAAGAGGA AAAACTTGAT 
CATGCGTACG AAAAACCGTT GACGTTCCCG CGGCCAAAGG CACAACTGAA GCGCACCAAG 
CGGGAGCCCA AGGTGCCGCG GCCACCGACT CGAGTTCAGC CGGAGAGGGC GCGGGCAATG 
CGTCAACTTT ATCAGTTCCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JIL-1 ( NM_001274768.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261697.1 Drosophila melanogaster JIL-1 kinase (JIL-1), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa18204
Clone ID Related Accession (Same CDS sequence)	NM_140175.3 , NM_001274768.1 , NM_168439.2
Accession Version	NM_001274768.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	JIL-1 kinase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTCGCT TGCAAAAGCA AAACTACGAG ATCTTAAGCG GTACAAGTAC GTCGCGCCTC 
AAGAATCATC AACATCCCCG TGAATCGGAG TCACTAGCCT ACGAGGAGCC AGATCAAATG 
GTGCGGAATC ATCTAAATGG CCAGCTGGTA GCCAATGGCA ATGGCAAAAC GCGAAAGAAT 
AGCAATTCCG AAACGATGAC CAATGGGAAA AAGTCCAAAC TGAATACGGA GGGATCCGGC 
TCTGGTTCTG GAAAGACCTT GAACTACAAT AATAATAACA ATAACAACAA CAGCATCAGT 
GCCACCAACG GTCAGTACAC CAATAGCAGC AGCAAGACCA CTTCAGCATC TGCAAGGGAT 
TACACTTACC GCGAGACCAT ATCACCGCCC ACACCTCCAT CCCCGCCCAC CACAAATGTG 
GCGGATATTG TTTGCATATC GGATGCGGAG AGCGAGGACG GACGCGATCC CGAGCGGGAG 
TACTACGACC AGGACATGGA GGAGGATGAG CCGAACGGGA TAGAGATCGA TGAATCCAGC 
TCCAGCTTGT CCAAGGCCAA GTCCAACAAT GCAGCTGCAG CGGCGGCGGC GGCGGCTGCA 
GCAGCGGCAG CAGCAGCCTC GAAGGCCTCC TCGTCGACCA CGCCCTCCTA CGCCATGCCC 
ACCAGCAATA GTACACCCCT TGATCTGGAC AATGAGGCCC ATCAACGGGA CCTGGAGGCG 
GTCACCGATC TGAAATACTA CGTAAAATTG TACAGTGATG AGGCCGTTAG TCTGAATGAC 
TTCAAAATAA TACGCGTTCT CGGAACAGGT GCCTATGGGC GGGTTTTCCT CGTTCGCAAG 
CTAACTAGAC ACGATGCCGG AAAATTGTAT GCAATGAAGG TTCTCAATAA GATAACGGTG 
GTCCAGAAGC GAAAAACCGC GGAACACACC AAAACGGAGA GAGTGGTACT GGAGGCCATT 
CAACGTAATC CCTTCCTCGT GAGTCTACAC TACGCCTTCC AATCCTCATC AAAGCTTTAC 
CTCGTGCTAG ACTTTGCCAA TGGCGGTGAG CTCTTCACGC ATCTCTACCA CTCTGAGAAC 
TTTGAGGAGT CCCGGGTGCG TGTGTACATA GCCGAGGTGG TGCTGGCCCT GGAGCAACTG 
CATCAGTTGG GGATCATCTA TCGAGATATT AAGTTGGAGA ATATTTTGCT TGATGGCGAG 
GGGCACATTG TTCTGTCCGA CTTTGGACTG TCGAAGATCC TTACCGCGGA GAATGAATAC 
CGTGCGCATA GCTTCTGCGG CACTCTGGAG TATATGGCAC CTGAGATAAT CCGTACGGGG 
CCGCCAGGAC ACGACAGTGC CGTGGACTGG TGGTCGGTGG GTGTGCTCAC TTTTGAGCTG 
CTCACCGGCG CTTCGCCATT CGCCACGTCC GACGGCCAGG TCCAGCAATC GGAAATCTCG 
CGGCGGATTC AGAAGGAGCA GCCAATGATT CCCTCATCGT TCAGTGCCAA CGCCCGCGAC 
TTTGTGCTAA AGATGTTGGA AAAGAATCCA AAGCGGCGCT TGGGTGGCAA CCATCGCGAC 
GCCAGCGAAA TCAAGGAGCA CCCCTTCTTC AACGGCATTA ACTGGCAGGA GTTGCGGACA 
AAGCGCCGCA AAGCCCCCTA TAAGCCCACC CTCACCGCCG AGGACGATGT GCAGAACTTC 
AGCAACGAGT TTACCGACCA AGTGCCCGAG GATCCCGAGT GCGATGCTCC GCCCAGCCGG 
ATACGACTAT TTCGCGGTTA CACATACGTG GCGCCAGAGC ATTTGGAGCA GATGCGAAGG 
GATAACCACT GTGAGATTCA ATACTTTAAT ACAGGACTTC AGAATATACC TTGTCGGCCA 
GATGATCTTG AATTGGGCAC ACGCACTTCA AACGGAGCCT ATGGCACATG CCATTTTGTG 
GTGGACAGCT CTACGGATTT GGTGTTTTTG GCCAAAATAA TACCGCTATC CAAGTTCCGC 
CCCTCCGAGG TCGACGCGCT GATCTCGTGC GCTCTCGACA CGACCAATCA CAAAAATATC 
GTAAGCTACC ATGGAACGTT CAGGGAAAAG TGCGAGACAT GGATCGTTAT GGAGTACCTG 
TCCGGCCCGG AGCTGACCGC ATCCATTCGC ATGGATGAGG ATTCGTGTCG GGAGATCTTT 
CTGCAGCTGG TCATGGCTGT GCGGCACATA CACTCCAAGC ACTTTATACA CGGTGACCTG 
AAACCGGAGA ACATAATGTT CGAGAACCGA GAGGATAGGA CTGTCAAACT GATCGATTTT 
GGCAGCGCAT GTTACAATAA TCGCTTTAAG AGCTGGAAGG ACAAGCCGCG CTACACGCTG 
GACTATGCTC CGCCAGAAAT GCTCGCGGAT GCGAATCTCG TTACTTATTC GCCAGCCGTG 
GATATATACG GTCTTGGGGC CACGCTGTAT ACCATGCTGG TGGGTCATCG GCCGTACAGA 
CAGAACGAAG ACGATGTGGA TCATTCCGCA GCGGCCCATC ACGAGCTTCG AAAGCGAATG 
CGCCGGGGAA CGTTTAATCA GCGTTCTATG CGCTGGGAGA GTGCAAGTCC TGCGTTCCGA 
CATTTGGTTA GCTGGTGTCT GCAGCGGGAT CCGGCTGATC GGCCCACATT GTCAGATATT 
CTCGACAGCG AGTGGCTGCA GTACGGCTCA AATGATCCGG ACGTGGATAT CATTCTGCCA 
CAACAGATGG TTGTGGACCT CAGCGAAGAC ACTATGGAAC AACCGACGGG AGGTATGTTC 
GACGATCAGC AACAACTGGA ATTCATGCAC GACAAGTCGG CGGAGGATGA GGGCATAACC 
CTAGTCAGCG AGCCCATGGA TACGACTGTC GCGACTCACG AGTCTAGGAG AAATGCAGCC 
GCATTTTCGT CGGTAGTTGC TCCAACCACC GACGACGAGA TAGTGCACGA ACGATTTGAC 
CCGGCATTTG AGGTCCAAGC GGACTTCTAC GGTTTCGACG AGAATGCGCC GCCTCTGCCT 
CTGCCCGAGG AGTACTACTC CGAACTACCT CTGCCAGAGG AGGACCGGCA GTACATACCA 
CCTCCACCAG CACTGATTCC TGTTGAACCA GAAACGACCT TCCGACGGCC AAGAACACGC 
CAGCAGCGTC GCACTGAAAG CCAGCTAGTA CAACCTGTTT CCGTCGCTAC GTATGAGGAC 
AGCAAGGCGT CCCTGCGCGT ACTAATGCAA CAGCTGCCGC CGCCAGGTGA CAATGTGGTA 
GCACGCATTC CGAAGAGAAC ACATCGAGTC GTACGTACAT TGCCTCCGAC CTTTGGAACC 
ACCAAGCGAG AAGAGAATTT CTATGGATTT AGTAAGACAG CAATATCGTG GCGAAAGACT 
CGGGCCAGCT GGCGTCACTT CTGTCTGCTT ATCAATGGTG TGCAGCAGGT GCTTAAGGTT 
CGGTTCAAAA AAGCGCGTCG CGTCTACTGC CTGCCCCATA TAAAAGAGGA AAAACTTGAT 
CATGCGTACG AAAAACCGTT GACGTTCCCG CGGCCAAAGG CACAACTGAA GCGCACCAAG 
CGGGAGCCCA AGGTGCCGCG GCCACCGACT CGAGTTCAGC CGGAGAGGGC GCGGGCAATG 
CGTCAACTTT ATCAGTTCCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261697.1
CDS	1056..4679
Translation	MSRLQKQNYEILSGTSTSRLKNHQHPRESESLAYEEPDQMVRNHLNGQLVANGNGKTRKNSNSETMTNGKKSKLNTEGSGSGSGKTLNYNNNNNNNNSISATNGQYTNSSSKTTSASARDYTYRETISPPTPPSPPTTNVADIVCISDAESEDGRDPEREYYDQDMEEDEPNGIEIDESSSSLSKAKSNNAAAAAAAAAAAAAAAASKASSSTTPSYAMPTSNSTPLDLDNEAHQRDLEAVTDLKYYVKLYSDEAVSLNDFKIIRVLGTGAYGRVFLVRKLTRHDAGKLYAMKVLNKITVVQKRKTAEHTKTERVVLEAIQRNPFLVSLHYAFQSSSKLYLVLDFANGGELFTHLYHSENFEESRVRVYIAEVVLALEQLHQLGIIYRDIKLENILLDGEGHIVLSDFGLSKILTAENEYRAHSFCGTLEYMAPEIIRTGPPGHDSAVDWWSVGVLTFELLTGASPFATSDGQVQQSEISRRIQKEQPMIPSSFSANARDFVLKMLEKNPKRRLGGNHRDASEIKEHPFFNGINWQELRTKRRKAPYKPTLTAEDDVQNFSNEFTDQVPEDPECDAPPSRIRLFRGYTYVAPEHLEQMRRDNHCEIQYFNTGLQNIPCRPDDLELGTRTSNGAYGTCHFVVDSSTDLVFLAKIIPLSKFRPSEVDALISCALDTTNHKNIVSYHGTFREKCETWIVMEYLSGPELTASIRMDEDSCREIFLQLVMAVRHIHSKHFIHGDLKPENIMFENREDRTVKLIDFGSACYNNRFKSWKDKPRYTLDYAPPEMLADANLVTYSPAVDIYGLGATLYTMLVGHRPYRQNEDDVDHSAAAHHELRKRMRRGTFNQRSMRWESASPAFRHLVSWCLQRDPADRPTLSDILDSEWLQYGSNDPDVDIILPQQMVVDLSEDTMEQPTGGMFDDQQQLEFMHDKSAEDEGITLVSEPMDTTVATHESRRNAAAFSSVVAPTTDDEIVHERFDPAFEVQADFYGFDENAPPLPLPEEYYSELPLPEEDRQYIPPPPALIPVEPETTFRRPRTRQQRRTESQLVQPVSVATYEDSKASLRVLMQQLPPPGDNVVARIPKRTHRVVRTLPPTFGTTKREENFYGFSKTAISWRKTRASWRHFCLLINGVQQVLKVRFKKARRVYCLPHIKEEKLDHAYEKPLTFPRPKAQLKRTKREPKVPRPPTRVQPERARAMRQLYQFQ

Target ORF information:

RefSeq Version	NM_001274768.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster JIL-1 kinase (JIL-1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274768.1

ORF Insert Sequence:

ATGAGTCGCT TGCAAAAGCA AAACTACGAG ATCTTAAGCG GTACAAGTAC GTCGCGCCTC 
AAGAATCATC AACATCCCCG TGAATCGGAG TCACTAGCCT ACGAGGAGCC AGATCAAATG 
GTGCGGAATC ATCTAAATGG CCAGCTGGTA GCCAATGGCA ATGGCAAAAC GCGAAAGAAT 
AGCAATTCCG AAACGATGAC CAATGGGAAA AAGTCCAAAC TGAATACGGA GGGATCCGGC 
TCTGGTTCTG GAAAGACCTT GAACTACAAT AATAATAACA ATAACAACAA CAGCATCAGT 
GCCACCAACG GTCAGTACAC CAATAGCAGC AGCAAGACCA CTTCAGCATC TGCAAGGGAT 
TACACTTACC GCGAGACCAT ATCACCGCCC ACACCTCCAT CCCCGCCCAC CACAAATGTG 
GCGGATATTG TTTGCATATC GGATGCGGAG AGCGAGGACG GACGCGATCC CGAGCGGGAG 
TACTACGACC AGGACATGGA GGAGGATGAG CCGAACGGGA TAGAGATCGA TGAATCCAGC 
TCCAGCTTGT CCAAGGCCAA GTCCAACAAT GCAGCTGCAG CGGCGGCGGC GGCGGCTGCA 
GCAGCGGCAG CAGCAGCCTC GAAGGCCTCC TCGTCGACCA CGCCCTCCTA CGCCATGCCC 
ACCAGCAATA GTACACCCCT TGATCTGGAC AATGAGGCCC ATCAACGGGA CCTGGAGGCG 
GTCACCGATC TGAAATACTA CGTAAAATTG TACAGTGATG AGGCCGTTAG TCTGAATGAC 
TTCAAAATAA TACGCGTTCT CGGAACAGGT GCCTATGGGC GGGTTTTCCT CGTTCGCAAG 
CTAACTAGAC ACGATGCCGG AAAATTGTAT GCAATGAAGG TTCTCAATAA GATAACGGTG 
GTCCAGAAGC GAAAAACCGC GGAACACACC AAAACGGAGA GAGTGGTACT GGAGGCCATT 
CAACGTAATC CCTTCCTCGT GAGTCTACAC TACGCCTTCC AATCCTCATC AAAGCTTTAC 
CTCGTGCTAG ACTTTGCCAA TGGCGGTGAG CTCTTCACGC ATCTCTACCA CTCTGAGAAC 
TTTGAGGAGT CCCGGGTGCG TGTGTACATA GCCGAGGTGG TGCTGGCCCT GGAGCAACTG 
CATCAGTTGG GGATCATCTA TCGAGATATT AAGTTGGAGA ATATTTTGCT TGATGGCGAG 
GGGCACATTG TTCTGTCCGA CTTTGGACTG TCGAAGATCC TTACCGCGGA GAATGAATAC 
CGTGCGCATA GCTTCTGCGG CACTCTGGAG TATATGGCAC CTGAGATAAT CCGTACGGGG 
CCGCCAGGAC ACGACAGTGC CGTGGACTGG TGGTCGGTGG GTGTGCTCAC TTTTGAGCTG 
CTCACCGGCG CTTCGCCATT CGCCACGTCC GACGGCCAGG TCCAGCAATC GGAAATCTCG 
CGGCGGATTC AGAAGGAGCA GCCAATGATT CCCTCATCGT TCAGTGCCAA CGCCCGCGAC 
TTTGTGCTAA AGATGTTGGA AAAGAATCCA AAGCGGCGCT TGGGTGGCAA CCATCGCGAC 
GCCAGCGAAA TCAAGGAGCA CCCCTTCTTC AACGGCATTA ACTGGCAGGA GTTGCGGACA 
AAGCGCCGCA AAGCCCCCTA TAAGCCCACC CTCACCGCCG AGGACGATGT GCAGAACTTC 
AGCAACGAGT TTACCGACCA AGTGCCCGAG GATCCCGAGT GCGATGCTCC GCCCAGCCGG 
ATACGACTAT TTCGCGGTTA CACATACGTG GCGCCAGAGC ATTTGGAGCA GATGCGAAGG 
GATAACCACT GTGAGATTCA ATACTTTAAT ACAGGACTTC AGAATATACC TTGTCGGCCA 
GATGATCTTG AATTGGGCAC ACGCACTTCA AACGGAGCCT ATGGCACATG CCATTTTGTG 
GTGGACAGCT CTACGGATTT GGTGTTTTTG GCCAAAATAA TACCGCTATC CAAGTTCCGC 
CCCTCCGAGG TCGACGCGCT GATCTCGTGC GCTCTCGACA CGACCAATCA CAAAAATATC 
GTAAGCTACC ATGGAACGTT CAGGGAAAAG TGCGAGACAT GGATCGTTAT GGAGTACCTG 
TCCGGCCCGG AGCTGACCGC ATCCATTCGC ATGGATGAGG ATTCGTGTCG GGAGATCTTT 
CTGCAGCTGG TCATGGCTGT GCGGCACATA CACTCCAAGC ACTTTATACA CGGTGACCTG 
AAACCGGAGA ACATAATGTT CGAGAACCGA GAGGATAGGA CTGTCAAACT GATCGATTTT 
GGCAGCGCAT GTTACAATAA TCGCTTTAAG AGCTGGAAGG ACAAGCCGCG CTACACGCTG 
GACTATGCTC CGCCAGAAAT GCTCGCGGAT GCGAATCTCG TTACTTATTC GCCAGCCGTG 
GATATATACG GTCTTGGGGC CACGCTGTAT ACCATGCTGG TGGGTCATCG GCCGTACAGA 
CAGAACGAAG ACGATGTGGA TCATTCCGCA GCGGCCCATC ACGAGCTTCG AAAGCGAATG 
CGCCGGGGAA CGTTTAATCA GCGTTCTATG CGCTGGGAGA GTGCAAGTCC TGCGTTCCGA 
CATTTGGTTA GCTGGTGTCT GCAGCGGGAT CCGGCTGATC GGCCCACATT GTCAGATATT 
CTCGACAGCG AGTGGCTGCA GTACGGCTCA AATGATCCGG ACGTGGATAT CATTCTGCCA 
CAACAGATGG TTGTGGACCT CAGCGAAGAC ACTATGGAAC AACCGACGGG AGGTATGTTC 
GACGATCAGC AACAACTGGA ATTCATGCAC GACAAGTCGG CGGAGGATGA GGGCATAACC 
CTAGTCAGCG AGCCCATGGA TACGACTGTC GCGACTCACG AGTCTAGGAG AAATGCAGCC 
GCATTTTCGT CGGTAGTTGC TCCAACCACC GACGACGAGA TAGTGCACGA ACGATTTGAC 
CCGGCATTTG AGGTCCAAGC GGACTTCTAC GGTTTCGACG AGAATGCGCC GCCTCTGCCT 
CTGCCCGAGG AGTACTACTC CGAACTACCT CTGCCAGAGG AGGACCGGCA GTACATACCA 
CCTCCACCAG CACTGATTCC TGTTGAACCA GAAACGACCT TCCGACGGCC AAGAACACGC 
CAGCAGCGTC GCACTGAAAG CCAGCTAGTA CAACCTGTTT CCGTCGCTAC GTATGAGGAC 
AGCAAGGCGT CCCTGCGCGT ACTAATGCAA CAGCTGCCGC CGCCAGGTGA CAATGTGGTA 
GCACGCATTC CGAAGAGAAC ACATCGAGTC GTACGTACAT TGCCTCCGAC CTTTGGAACC 
ACCAAGCGAG AAGAGAATTT CTATGGATTT AGTAAGACAG CAATATCGTG GCGAAAGACT 
CGGGCCAGCT GGCGTCACTT CTGTCTGCTT ATCAATGGTG TGCAGCAGGT GCTTAAGGTT 
CGGTTCAAAA AAGCGCGTCG CGTCTACTGC CTGCCCCATA TAAAAGAGGA AAAACTTGAT 
CATGCGTACG AAAAACCGTT GACGTTCCCG CGGCCAAAGG CACAACTGAA GCGCACCAAG 
CGGGAGCCCA AGGTGCCGCG GCCACCGACT CGAGTTCAGC CGGAGAGGGC GCGGGCAATG 
CGTCAACTTT ATCAGTTCCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

JIL-1 ( NM_168439.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729661.1 Drosophila melanogaster JIL-1 kinase (JIL-1), transcript variant A, mRNA.

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CloneID	OFa18204
Clone ID Related Accession (Same CDS sequence)	NM_140175.3 , NM_001274768.1 , NM_168439.2
Accession Version	NM_168439.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	JIL-1 kinase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168439.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTCGCT TGCAAAAGCA AAACTACGAG ATCTTAAGCG GTACAAGTAC GTCGCGCCTC 
AAGAATCATC AACATCCCCG TGAATCGGAG TCACTAGCCT ACGAGGAGCC AGATCAAATG 
GTGCGGAATC ATCTAAATGG CCAGCTGGTA GCCAATGGCA ATGGCAAAAC GCGAAAGAAT 
AGCAATTCCG AAACGATGAC CAATGGGAAA AAGTCCAAAC TGAATACGGA GGGATCCGGC 
TCTGGTTCTG GAAAGACCTT GAACTACAAT AATAATAACA ATAACAACAA CAGCATCAGT 
GCCACCAACG GTCAGTACAC CAATAGCAGC AGCAAGACCA CTTCAGCATC TGCAAGGGAT 
TACACTTACC GCGAGACCAT ATCACCGCCC ACACCTCCAT CCCCGCCCAC CACAAATGTG 
GCGGATATTG TTTGCATATC GGATGCGGAG AGCGAGGACG GACGCGATCC CGAGCGGGAG 
TACTACGACC AGGACATGGA GGAGGATGAG CCGAACGGGA TAGAGATCGA TGAATCCAGC 
TCCAGCTTGT CCAAGGCCAA GTCCAACAAT GCAGCTGCAG CGGCGGCGGC GGCGGCTGCA 
GCAGCGGCAG CAGCAGCCTC GAAGGCCTCC TCGTCGACCA CGCCCTCCTA CGCCATGCCC 
ACCAGCAATA GTACACCCCT TGATCTGGAC AATGAGGCCC ATCAACGGGA CCTGGAGGCG 
GTCACCGATC TGAAATACTA CGTAAAATTG TACAGTGATG AGGCCGTTAG TCTGAATGAC 
TTCAAAATAA TACGCGTTCT CGGAACAGGT GCCTATGGGC GGGTTTTCCT CGTTCGCAAG 
CTAACTAGAC ACGATGCCGG AAAATTGTAT GCAATGAAGG TTCTCAATAA GATAACGGTG 
GTCCAGAAGC GAAAAACCGC GGAACACACC AAAACGGAGA GAGTGGTACT GGAGGCCATT 
CAACGTAATC CCTTCCTCGT GAGTCTACAC TACGCCTTCC AATCCTCATC AAAGCTTTAC 
CTCGTGCTAG ACTTTGCCAA TGGCGGTGAG CTCTTCACGC ATCTCTACCA CTCTGAGAAC 
TTTGAGGAGT CCCGGGTGCG TGTGTACATA GCCGAGGTGG TGCTGGCCCT GGAGCAACTG 
CATCAGTTGG GGATCATCTA TCGAGATATT AAGTTGGAGA ATATTTTGCT TGATGGCGAG 
GGGCACATTG TTCTGTCCGA CTTTGGACTG TCGAAGATCC TTACCGCGGA GAATGAATAC 
CGTGCGCATA GCTTCTGCGG CACTCTGGAG TATATGGCAC CTGAGATAAT CCGTACGGGG 
CCGCCAGGAC ACGACAGTGC CGTGGACTGG TGGTCGGTGG GTGTGCTCAC TTTTGAGCTG 
CTCACCGGCG CTTCGCCATT CGCCACGTCC GACGGCCAGG TCCAGCAATC GGAAATCTCG 
CGGCGGATTC AGAAGGAGCA GCCAATGATT CCCTCATCGT TCAGTGCCAA CGCCCGCGAC 
TTTGTGCTAA AGATGTTGGA AAAGAATCCA AAGCGGCGCT TGGGTGGCAA CCATCGCGAC 
GCCAGCGAAA TCAAGGAGCA CCCCTTCTTC AACGGCATTA ACTGGCAGGA GTTGCGGACA 
AAGCGCCGCA AAGCCCCCTA TAAGCCCACC CTCACCGCCG AGGACGATGT GCAGAACTTC 
AGCAACGAGT TTACCGACCA AGTGCCCGAG GATCCCGAGT GCGATGCTCC GCCCAGCCGG 
ATACGACTAT TTCGCGGTTA CACATACGTG GCGCCAGAGC ATTTGGAGCA GATGCGAAGG 
GATAACCACT GTGAGATTCA ATACTTTAAT ACAGGACTTC AGAATATACC TTGTCGGCCA 
GATGATCTTG AATTGGGCAC ACGCACTTCA AACGGAGCCT ATGGCACATG CCATTTTGTG 
GTGGACAGCT CTACGGATTT GGTGTTTTTG GCCAAAATAA TACCGCTATC CAAGTTCCGC 
CCCTCCGAGG TCGACGCGCT GATCTCGTGC GCTCTCGACA CGACCAATCA CAAAAATATC 
GTAAGCTACC ATGGAACGTT CAGGGAAAAG TGCGAGACAT GGATCGTTAT GGAGTACCTG 
TCCGGCCCGG AGCTGACCGC ATCCATTCGC ATGGATGAGG ATTCGTGTCG GGAGATCTTT 
CTGCAGCTGG TCATGGCTGT GCGGCACATA CACTCCAAGC ACTTTATACA CGGTGACCTG 
AAACCGGAGA ACATAATGTT CGAGAACCGA GAGGATAGGA CTGTCAAACT GATCGATTTT 
GGCAGCGCAT GTTACAATAA TCGCTTTAAG AGCTGGAAGG ACAAGCCGCG CTACACGCTG 
GACTATGCTC CGCCAGAAAT GCTCGCGGAT GCGAATCTCG TTACTTATTC GCCAGCCGTG 
GATATATACG GTCTTGGGGC CACGCTGTAT ACCATGCTGG TGGGTCATCG GCCGTACAGA 
CAGAACGAAG ACGATGTGGA TCATTCCGCA GCGGCCCATC ACGAGCTTCG AAAGCGAATG 
CGCCGGGGAA CGTTTAATCA GCGTTCTATG CGCTGGGAGA GTGCAAGTCC TGCGTTCCGA 
CATTTGGTTA GCTGGTGTCT GCAGCGGGAT CCGGCTGATC GGCCCACATT GTCAGATATT 
CTCGACAGCG AGTGGCTGCA GTACGGCTCA AATGATCCGG ACGTGGATAT CATTCTGCCA 
CAACAGATGG TTGTGGACCT CAGCGAAGAC ACTATGGAAC AACCGACGGG AGGTATGTTC 
GACGATCAGC AACAACTGGA ATTCATGCAC GACAAGTCGG CGGAGGATGA GGGCATAACC 
CTAGTCAGCG AGCCCATGGA TACGACTGTC GCGACTCACG AGTCTAGGAG AAATGCAGCC 
GCATTTTCGT CGGTAGTTGC TCCAACCACC GACGACGAGA TAGTGCACGA ACGATTTGAC 
CCGGCATTTG AGGTCCAAGC GGACTTCTAC GGTTTCGACG AGAATGCGCC GCCTCTGCCT 
CTGCCCGAGG AGTACTACTC CGAACTACCT CTGCCAGAGG AGGACCGGCA GTACATACCA 
CCTCCACCAG CACTGATTCC TGTTGAACCA GAAACGACCT TCCGACGGCC AAGAACACGC 
CAGCAGCGTC GCACTGAAAG CCAGCTAGTA CAACCTGTTT CCGTCGCTAC GTATGAGGAC 
AGCAAGGCGT CCCTGCGCGT ACTAATGCAA CAGCTGCCGC CGCCAGGTGA CAATGTGGTA 
GCACGCATTC CGAAGAGAAC ACATCGAGTC GTACGTACAT TGCCTCCGAC CTTTGGAACC 
ACCAAGCGAG AAGAGAATTT CTATGGATTT AGTAAGACAG CAATATCGTG GCGAAAGACT 
CGGGCCAGCT GGCGTCACTT CTGTCTGCTT ATCAATGGTG TGCAGCAGGT GCTTAAGGTT 
CGGTTCAAAA AAGCGCGTCG CGTCTACTGC CTGCCCCATA TAAAAGAGGA AAAACTTGAT 
CATGCGTACG AAAAACCGTT GACGTTCCCG CGGCCAAAGG CACAACTGAA GCGCACCAAG 
CGGGAGCCCA AGGTGCCGCG GCCACCGACT CGAGTTCAGC CGGAGAGGGC GCGGGCAATG 
CGTCAACTTT ATCAGTTCCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729661.1
CDS	218..3841
Translation	MSRLQKQNYEILSGTSTSRLKNHQHPRESESLAYEEPDQMVRNHLNGQLVANGNGKTRKNSNSETMTNGKKSKLNTEGSGSGSGKTLNYNNNNNNNNSISATNGQYTNSSSKTTSASARDYTYRETISPPTPPSPPTTNVADIVCISDAESEDGRDPEREYYDQDMEEDEPNGIEIDESSSSLSKAKSNNAAAAAAAAAAAAAAAASKASSSTTPSYAMPTSNSTPLDLDNEAHQRDLEAVTDLKYYVKLYSDEAVSLNDFKIIRVLGTGAYGRVFLVRKLTRHDAGKLYAMKVLNKITVVQKRKTAEHTKTERVVLEAIQRNPFLVSLHYAFQSSSKLYLVLDFANGGELFTHLYHSENFEESRVRVYIAEVVLALEQLHQLGIIYRDIKLENILLDGEGHIVLSDFGLSKILTAENEYRAHSFCGTLEYMAPEIIRTGPPGHDSAVDWWSVGVLTFELLTGASPFATSDGQVQQSEISRRIQKEQPMIPSSFSANARDFVLKMLEKNPKRRLGGNHRDASEIKEHPFFNGINWQELRTKRRKAPYKPTLTAEDDVQNFSNEFTDQVPEDPECDAPPSRIRLFRGYTYVAPEHLEQMRRDNHCEIQYFNTGLQNIPCRPDDLELGTRTSNGAYGTCHFVVDSSTDLVFLAKIIPLSKFRPSEVDALISCALDTTNHKNIVSYHGTFREKCETWIVMEYLSGPELTASIRMDEDSCREIFLQLVMAVRHIHSKHFIHGDLKPENIMFENREDRTVKLIDFGSACYNNRFKSWKDKPRYTLDYAPPEMLADANLVTYSPAVDIYGLGATLYTMLVGHRPYRQNEDDVDHSAAAHHELRKRMRRGTFNQRSMRWESASPAFRHLVSWCLQRDPADRPTLSDILDSEWLQYGSNDPDVDIILPQQMVVDLSEDTMEQPTGGMFDDQQQLEFMHDKSAEDEGITLVSEPMDTTVATHESRRNAAAFSSVVAPTTDDEIVHERFDPAFEVQADFYGFDENAPPLPLPEEYYSELPLPEEDRQYIPPPPALIPVEPETTFRRPRTRQQRRTESQLVQPVSVATYEDSKASLRVLMQQLPPPGDNVVARIPKRTHRVVRTLPPTFGTTKREENFYGFSKTAISWRKTRASWRHFCLLINGVQQVLKVRFKKARRVYCLPHIKEEKLDHAYEKPLTFPRPKAQLKRTKREPKVPRPPTRVQPERARAMRQLYQFQ

Target ORF information:

RefSeq Version	NM_168439.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster JIL-1 kinase (JIL-1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168439.2

ORF Insert Sequence:

ATGAGTCGCT TGCAAAAGCA AAACTACGAG ATCTTAAGCG GTACAAGTAC GTCGCGCCTC 
AAGAATCATC AACATCCCCG TGAATCGGAG TCACTAGCCT ACGAGGAGCC AGATCAAATG 
GTGCGGAATC ATCTAAATGG CCAGCTGGTA GCCAATGGCA ATGGCAAAAC GCGAAAGAAT 
AGCAATTCCG AAACGATGAC CAATGGGAAA AAGTCCAAAC TGAATACGGA GGGATCCGGC 
TCTGGTTCTG GAAAGACCTT GAACTACAAT AATAATAACA ATAACAACAA CAGCATCAGT 
GCCACCAACG GTCAGTACAC CAATAGCAGC AGCAAGACCA CTTCAGCATC TGCAAGGGAT 
TACACTTACC GCGAGACCAT ATCACCGCCC ACACCTCCAT CCCCGCCCAC CACAAATGTG 
GCGGATATTG TTTGCATATC GGATGCGGAG AGCGAGGACG GACGCGATCC CGAGCGGGAG 
TACTACGACC AGGACATGGA GGAGGATGAG CCGAACGGGA TAGAGATCGA TGAATCCAGC 
TCCAGCTTGT CCAAGGCCAA GTCCAACAAT GCAGCTGCAG CGGCGGCGGC GGCGGCTGCA 
GCAGCGGCAG CAGCAGCCTC GAAGGCCTCC TCGTCGACCA CGCCCTCCTA CGCCATGCCC 
ACCAGCAATA GTACACCCCT TGATCTGGAC AATGAGGCCC ATCAACGGGA CCTGGAGGCG 
GTCACCGATC TGAAATACTA CGTAAAATTG TACAGTGATG AGGCCGTTAG TCTGAATGAC 
TTCAAAATAA TACGCGTTCT CGGAACAGGT GCCTATGGGC GGGTTTTCCT CGTTCGCAAG 
CTAACTAGAC ACGATGCCGG AAAATTGTAT GCAATGAAGG TTCTCAATAA GATAACGGTG 
GTCCAGAAGC GAAAAACCGC GGAACACACC AAAACGGAGA GAGTGGTACT GGAGGCCATT 
CAACGTAATC CCTTCCTCGT GAGTCTACAC TACGCCTTCC AATCCTCATC AAAGCTTTAC 
CTCGTGCTAG ACTTTGCCAA TGGCGGTGAG CTCTTCACGC ATCTCTACCA CTCTGAGAAC 
TTTGAGGAGT CCCGGGTGCG TGTGTACATA GCCGAGGTGG TGCTGGCCCT GGAGCAACTG 
CATCAGTTGG GGATCATCTA TCGAGATATT AAGTTGGAGA ATATTTTGCT TGATGGCGAG 
GGGCACATTG TTCTGTCCGA CTTTGGACTG TCGAAGATCC TTACCGCGGA GAATGAATAC 
CGTGCGCATA GCTTCTGCGG CACTCTGGAG TATATGGCAC CTGAGATAAT CCGTACGGGG 
CCGCCAGGAC ACGACAGTGC CGTGGACTGG TGGTCGGTGG GTGTGCTCAC TTTTGAGCTG 
CTCACCGGCG CTTCGCCATT CGCCACGTCC GACGGCCAGG TCCAGCAATC GGAAATCTCG 
CGGCGGATTC AGAAGGAGCA GCCAATGATT CCCTCATCGT TCAGTGCCAA CGCCCGCGAC 
TTTGTGCTAA AGATGTTGGA AAAGAATCCA AAGCGGCGCT TGGGTGGCAA CCATCGCGAC 
GCCAGCGAAA TCAAGGAGCA CCCCTTCTTC AACGGCATTA ACTGGCAGGA GTTGCGGACA 
AAGCGCCGCA AAGCCCCCTA TAAGCCCACC CTCACCGCCG AGGACGATGT GCAGAACTTC 
AGCAACGAGT TTACCGACCA AGTGCCCGAG GATCCCGAGT GCGATGCTCC GCCCAGCCGG 
ATACGACTAT TTCGCGGTTA CACATACGTG GCGCCAGAGC ATTTGGAGCA GATGCGAAGG 
GATAACCACT GTGAGATTCA ATACTTTAAT ACAGGACTTC AGAATATACC TTGTCGGCCA 
GATGATCTTG AATTGGGCAC ACGCACTTCA AACGGAGCCT ATGGCACATG CCATTTTGTG 
GTGGACAGCT CTACGGATTT GGTGTTTTTG GCCAAAATAA TACCGCTATC CAAGTTCCGC 
CCCTCCGAGG TCGACGCGCT GATCTCGTGC GCTCTCGACA CGACCAATCA CAAAAATATC 
GTAAGCTACC ATGGAACGTT CAGGGAAAAG TGCGAGACAT GGATCGTTAT GGAGTACCTG 
TCCGGCCCGG AGCTGACCGC ATCCATTCGC ATGGATGAGG ATTCGTGTCG GGAGATCTTT 
CTGCAGCTGG TCATGGCTGT GCGGCACATA CACTCCAAGC ACTTTATACA CGGTGACCTG 
AAACCGGAGA ACATAATGTT CGAGAACCGA GAGGATAGGA CTGTCAAACT GATCGATTTT 
GGCAGCGCAT GTTACAATAA TCGCTTTAAG AGCTGGAAGG ACAAGCCGCG CTACACGCTG 
GACTATGCTC CGCCAGAAAT GCTCGCGGAT GCGAATCTCG TTACTTATTC GCCAGCCGTG 
GATATATACG GTCTTGGGGC CACGCTGTAT ACCATGCTGG TGGGTCATCG GCCGTACAGA 
CAGAACGAAG ACGATGTGGA TCATTCCGCA GCGGCCCATC ACGAGCTTCG AAAGCGAATG 
CGCCGGGGAA CGTTTAATCA GCGTTCTATG CGCTGGGAGA GTGCAAGTCC TGCGTTCCGA 
CATTTGGTTA GCTGGTGTCT GCAGCGGGAT CCGGCTGATC GGCCCACATT GTCAGATATT 
CTCGACAGCG AGTGGCTGCA GTACGGCTCA AATGATCCGG ACGTGGATAT CATTCTGCCA 
CAACAGATGG TTGTGGACCT CAGCGAAGAC ACTATGGAAC AACCGACGGG AGGTATGTTC 
GACGATCAGC AACAACTGGA ATTCATGCAC GACAAGTCGG CGGAGGATGA GGGCATAACC 
CTAGTCAGCG AGCCCATGGA TACGACTGTC GCGACTCACG AGTCTAGGAG AAATGCAGCC 
GCATTTTCGT CGGTAGTTGC TCCAACCACC GACGACGAGA TAGTGCACGA ACGATTTGAC 
CCGGCATTTG AGGTCCAAGC GGACTTCTAC GGTTTCGACG AGAATGCGCC GCCTCTGCCT 
CTGCCCGAGG AGTACTACTC CGAACTACCT CTGCCAGAGG AGGACCGGCA GTACATACCA 
CCTCCACCAG CACTGATTCC TGTTGAACCA GAAACGACCT TCCGACGGCC AAGAACACGC 
CAGCAGCGTC GCACTGAAAG CCAGCTAGTA CAACCTGTTT CCGTCGCTAC GTATGAGGAC 
AGCAAGGCGT CCCTGCGCGT ACTAATGCAA CAGCTGCCGC CGCCAGGTGA CAATGTGGTA 
GCACGCATTC CGAAGAGAAC ACATCGAGTC GTACGTACAT TGCCTCCGAC CTTTGGAACC 
ACCAAGCGAG AAGAGAATTT CTATGGATTT AGTAAGACAG CAATATCGTG GCGAAAGACT 
CGGGCCAGCT GGCGTCACTT CTGTCTGCTT ATCAATGGTG TGCAGCAGGT GCTTAAGGTT 
CGGTTCAAAA AAGCGCGTCG CGTCTACTGC CTGCCCCATA TAAAAGAGGA AAAACTTGAT 
CATGCGTACG AAAAACCGTT GACGTTCCCG CGGCCAAAGG CACAACTGAA GCGCACCAAG 
CGGGAGCCCA AGGTGCCGCG GCCACCGACT CGAGTTCAGC CGGAGAGGGC GCGGGCAATG 
CGTCAACTTT ATCAGTTCCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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JIL-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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