Gem3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gem3
Entrez Gene ID	39195
Full Name	Gemin3
Synonyms	BcDNA.LD05563,BcDNA:LD05563,CG6539,DHH1,Dhh1,DmRH22,Dmel\CG6539,dGem3,l(3)rL562
General protein information	Preferred Names Gemin3 Names CG6539 gene product from transcript CG6539-RB CG6539-PA CG6539-PB DEAD/DEAH RNA helicase 1 Gem3-PA Gem3-PB dGemin3 gemin3
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 67E3-67E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274749.1	NP_001261678.1	Gemin3, isoform B
NM_079295.4	NP_524019.1	Gemin3, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that Gemin3 was required for the completion of oogenesis and its loss led to egg polarity defects, oocyte mislocalisation, and abnormal chromosome morphology.
Title: Conserved requirement for DEAD-box RNA helicase Gemin3 in Drosophila oogenesis.

Gemin3 overexpression in Drosophila
Title: Gem formation upon constitutive Gemin3 overexpression in Drosophila.

Gemin2, Gemin3 and Gemin5 colocalise with SMN in U bodies
Title: Drosophila SMN complex proteins Gemin2, Gemin3, and Gemin5 are components of U bodies.

Results demonstrate the conservation of Gemin3 (Gem3) protein function in metazoan snRNP assembly and reveal that loss of either survival motor neuron (Smn) or Gemin3 can contribute to neuromuscular dysfunction.
Title: Gemin3 is an essential gene required for larval motor function and pupation in Drosophila.

Drosophila Gemin3 is required for larval development and motor function.
Title: A motor function for the DEAD-box RNA helicase, Gemin3, in Drosophila.

The following Gem3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gem3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18151	NM_079295.4 Latest version!	Drosophila melanogaster Gemin3 (Gem3), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa18151	NM_001274749.1 Latest version!	Drosophila melanogaster Gemin3 (Gem3), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gem3 ( NM_001274749.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261678.1 Drosophila melanogaster Gemin3 (Gem3), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa18151
Clone ID Related Accession (Same CDS sequence)	NM_001274749.1 , NM_079295.4
Accession Version	NM_001274749.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Gemin3, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCGCG AAATAGCGCA CAGTTTAGCT GGCGGCGAGG AACGAAGTAG CGATGTGGCA 
CCTGGACAGG TTAAGACCTT CGAGGAACTG CGTCTATACC GAAATCTGTT GAACGGATTG 
AAGCGCAACA ATTTCGTGAC GCCAACGAAG ATTCAGGCAG CTGCCATACC CATGGCACTG 
GCCAAAATGG ATCTAATAAT ACAATCGAAG AGTGGAACTG GCAAAACTCT AATCTATGTT 
ATAGCCGTCG TTCAGAGCTT TAATCCCAAC ATAAACCAAC CGCATGCCAT GATCGTGGTC 
CCAACTCGAG AGCTGGCCAT TCAGGTGCAG GACACATTTT TCCATCTCTG CAAATCGTTT 
CGGGACTTTA AATGCTCTGC TTTCATCGGT GGCACCGATG TGGCCAAGGA TCGCAAGCGA 
ATGAATGAAA GTCGAGTCAT TATCGGTACA CCGGGACGTT TGCTGCATCT GTATGAGAAT 
AGAGTATTCG ATGTGTCCAA ACTGCGACTG CTGGTGCTCG ACGAGGCCGA TCAACTTTAT 
CAGACAAAGA GCCTGCAGCA TACGGTCAGT AAACTCATTG AGGCAATGCC CAAGAATCGG 
CAAATAATTG CCTGCAGTGC CACATACGAT CAGAATTTGG ATGAGCGTCT GGCCAAGGTC 
ATGGACAAAC CCATGCTTAT ATCGAATTCC GAAAGGGCTA CCGTTCTACT GGGCATTCGG 
CAGTTTGTCT ACGAATTGCC ACAGCAGAAT AACAGTGTGG AGGAGATGCG TCTTAAACTG 
CAGATTCTTG GCCAGATCTT TAATCAGCTG CCTTACGAAC AGGCCATCAT CTTTGCTAGC 
TCACAAATGC GAGCGGATTC TTACAAGAAC TATCTTACAG CCAGTGGTAT CGATTGTCAT 
TTAATTTCGG GAGCCATGGA ACAATCGGAG CGTTTGCATG TTTTCGAGGG CTATAGAAAC 
TTTACTATGC GCATCCTGGT GGCCACTGAT TTGATGGCAC GCGGAGTGGA CTCCCCGCAT 
GCCAACCTGG TTATCAACAT TGATCCACCA CAGGATCATG TCACCTATTT GCACCGCATT 
GGACGAGCTG GTCGGTTTGG CTCCAAAGGC ATAGCCATCA CGTTTATTGC CTCCAAGAAG 
GAGAGCCAGA GATTTAGGGA AATGTCAAAA AAAATCGCTA CGGCTTGGTC GGTTCTGGAG 
TTTCCCAAGG AGCCAATGCC CAATGAATTC AATTTTTGGG ATTTCGAAAA GTACAACTTT 
GATTATTACA TTAAGGAAGA GAATCCGCTA CAGGAAATGC CTATGCCTAT TAAAGAAAAT 
AGAAGTAAAG AAAATGTTGA TGCTTCTTCA GTTGACTTGG AAAATCTTCA AAAGGATCAG 
GATGGCAAAA GGAGAGATCC AGACAAACTA CCAGTGGCCT TAGAAAATGT TGAAACCCAA 
AAAGAGTTGG AGCTCGAAAA CTTACCAGAA AGTTCACATA ATAATAAGAA TCTTAGGGTC 
AAAGAGAAAG AAATTGTAAG GCAAGGAAAG CTTAAAGAAA CTAATTCCAA AGCTGGAGGT 
TCTAAAACGA ACAAGACGGA GAGAAGGAAA AAATCTAACA CTCCATCAAA GTTACAGAAA 
CAAATAACTG AAGTGCAGCA AACACCAGAG ATCACCGTAA ATAAATACTA TATGCAAGAA 
GACATACAAC GAACAGCAGA CGGCAAAGAG TATCATCCTG TTAATGGAGC GGGAAACCAC 
CAGGTTCCGT CAAAATCTAA TCAAAAACCG GTGGAGTTTA TATCTCCCGA ATTGACACCA 
ACCCTAACAC CATTGGAACT GACCCAGGAG CCGTCTCCAA CCACAGTGAC ACCACCTGCA 
CCGCCAGCCA ACTCGATTAA CAACAAAACC TATTGCCTAG CTGCTCCAAC GCAAACCAGT 
TCGATGACCA TACAGAACAT GGTAATATCG AACACGGTGG ACGATGCCAG TAGCATCAGT 
TCGGATAGCA TGGTGGTATG TGGCTATCAT AGCGATAGGT CCTACGACAC ATACTATGCA 
ACCAGTGATG AGGAGGAGAT CTGGAATAGA TTAATGTCGA AACAAAGGCG TCAGCTGAAG 
AGCAAGAGAG GCCATGGAAA ACGGCGTGTG ATACTATACA AGAAAGTATT TCCTAAACTA 
AAGGCGAATG TTAAACGCAA GCTTAAAAAA CGGATGAGCT CGTCATGCAG ATCCAAAAAA 
GCACACCATT TGCGAAAACG TCATGTATAC AAAAATATAT CACTTTTGCC ACACATGAAT 
TTGTGTCATC TGCTTCAAAA ATTTACAAAT CAAGAGAGAT TTTTGAAACG GCTCCACAAA 
TATGCAAAGA ATCAATTCAT TGACAATGCG AGGGTTGGCT TTTTGCTGGA TAAGCTCTAT 
AAATCCATCC TGGAAATGTA CTATAACAGT GCTAAGGAGA GGAAGAAGCA TTTTAAGGAA 
GCTATGAAGG AGTCCCTAAA ATCCATTGAT AACTACGAGA GCCAAGAGTC TTCGGAATCA 
GAGGAGGAGG AGGAGGAGGA GAAGGATCTA AAGAACAAGT TAAGTGTTCA TGTGACGAAT 
CAGATGGACA TACCACCAAT GCCACAGGCC GCTGTTGAAA TTGGTTCAGG AAGTGAATCG 
GATACGGAGG ACTCGCAGGA ATACGGCGAA GGCGAAGAGA TCATTGTGGA CGATGAGGAC 
GATGGCAGCG ATGGCGGGCC AAATTCATCG AGTGGTTTTG TAGAGAGTCA GGAGAGTGTT 
TCGTCTGGAA TAGACACTTC CGTTTACGAA ACGGAATCTA CGGGCGCATC TGGAAATAAT 
TCAAGTGCAT TCTTTATTTC GGATGATGAA TCAGATACTG AATCCTTAAC AGATTCGGAA 
GCTGGAAGCA ATGTCAGCGC GACAGGATCA AGCTCACAGC GATCTTCAGT AGCGAGCAGC 
ACCTATGAAT CTTCGGATGA TGATTCTGTA ACTGTAACCA ATGTCAGTTC AATGCAGAAC 
GCTCAATCAT TGTGGTTGCA GACTTTCAAC ATGCAGTACC AATTCATTGC ATCCCATGTG 
GCCAAGAATC TACAGAACTA TTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261678.1
CDS	76..3162
Translation	MEREIAHSLAGGEERSSDVAPGQVKTFEELRLYRNLLNGLKRNNFVTPTKIQAAAIPMALAKMDLIIQSKSGTGKTLIYVIAVVQSFNPNINQPHAMIVVPTRELAIQVQDTFFHLCKSFRDFKCSAFIGGTDVAKDRKRMNESRVIIGTPGRLLHLYENRVFDVSKLRLLVLDEADQLYQTKSLQHTVSKLIEAMPKNRQIIACSATYDQNLDERLAKVMDKPMLISNSERATVLLGIRQFVYELPQQNNSVEEMRLKLQILGQIFNQLPYEQAIIFASSQMRADSYKNYLTASGIDCHLISGAMEQSERLHVFEGYRNFTMRILVATDLMARGVDSPHANLVINIDPPQDHVTYLHRIGRAGRFGSKGIAITFIASKKESQRFREMSKKIATAWSVLEFPKEPMPNEFNFWDFEKYNFDYYIKEENPLQEMPMPIKENRSKENVDASSVDLENLQKDQDGKRRDPDKLPVALENVETQKELELENLPESSHNNKNLRVKEKEIVRQGKLKETNSKAGGSKTNKTERRKKSNTPSKLQKQITEVQQTPEITVNKYYMQEDIQRTADGKEYHPVNGAGNHQVPSKSNQKPVEFISPELTPTLTPLELTQEPSPTTVTPPAPPANSINNKTYCLAAPTQTSSMTIQNMVISNTVDDASSISSDSMVVCGYHSDRSYDTYYATSDEEEIWNRLMSKQRRQLKSKRGHGKRRVILYKKVFPKLKANVKRKLKKRMSSSCRSKKAHHLRKRHVYKNISLLPHMNLCHLLQKFTNQERFLKRLHKYAKNQFIDNARVGFLLDKLYKSILEMYYNSAKERKKHFKEAMKESLKSIDNYESQESSESEEEEEEEKDLKNKLSVHVTNQMDIPPMPQAAVEIGSGSESDTEDSQEYGEGEEIIVDDEDDGSDGGPNSSSGFVESQESVSSGIDTSVYETESTGASGNNSSAFFISDDESDTESLTDSEAGSNVSATGSSSQRSSVASSTYESSDDDSVTVTNVSSMQNAQSLWLQTFNMQYQFIASHVAKNLQNYY

Target ORF information:

RefSeq Version	NM_001274749.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Gemin3 (Gem3), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274749.1

ORF Insert Sequence:

ATGGAGCGCG AAATAGCGCA CAGTTTAGCT GGCGGCGAGG AACGAAGTAG CGATGTGGCA 
CCTGGACAGG TTAAGACCTT CGAGGAACTG CGTCTATACC GAAATCTGTT GAACGGATTG 
AAGCGCAACA ATTTCGTGAC GCCAACGAAG ATTCAGGCAG CTGCCATACC CATGGCACTG 
GCCAAAATGG ATCTAATAAT ACAATCGAAG AGTGGAACTG GCAAAACTCT AATCTATGTT 
ATAGCCGTCG TTCAGAGCTT TAATCCCAAC ATAAACCAAC CGCATGCCAT GATCGTGGTC 
CCAACTCGAG AGCTGGCCAT TCAGGTGCAG GACACATTTT TCCATCTCTG CAAATCGTTT 
CGGGACTTTA AATGCTCTGC TTTCATCGGT GGCACCGATG TGGCCAAGGA TCGCAAGCGA 
ATGAATGAAA GTCGAGTCAT TATCGGTACA CCGGGACGTT TGCTGCATCT GTATGAGAAT 
AGAGTATTCG ATGTGTCCAA ACTGCGACTG CTGGTGCTCG ACGAGGCCGA TCAACTTTAT 
CAGACAAAGA GCCTGCAGCA TACGGTCAGT AAACTCATTG AGGCAATGCC CAAGAATCGG 
CAAATAATTG CCTGCAGTGC CACATACGAT CAGAATTTGG ATGAGCGTCT GGCCAAGGTC 
ATGGACAAAC CCATGCTTAT ATCGAATTCC GAAAGGGCTA CCGTTCTACT GGGCATTCGG 
CAGTTTGTCT ACGAATTGCC ACAGCAGAAT AACAGTGTGG AGGAGATGCG TCTTAAACTG 
CAGATTCTTG GCCAGATCTT TAATCAGCTG CCTTACGAAC AGGCCATCAT CTTTGCTAGC 
TCACAAATGC GAGCGGATTC TTACAAGAAC TATCTTACAG CCAGTGGTAT CGATTGTCAT 
TTAATTTCGG GAGCCATGGA ACAATCGGAG CGTTTGCATG TTTTCGAGGG CTATAGAAAC 
TTTACTATGC GCATCCTGGT GGCCACTGAT TTGATGGCAC GCGGAGTGGA CTCCCCGCAT 
GCCAACCTGG TTATCAACAT TGATCCACCA CAGGATCATG TCACCTATTT GCACCGCATT 
GGACGAGCTG GTCGGTTTGG CTCCAAAGGC ATAGCCATCA CGTTTATTGC CTCCAAGAAG 
GAGAGCCAGA GATTTAGGGA AATGTCAAAA AAAATCGCTA CGGCTTGGTC GGTTCTGGAG 
TTTCCCAAGG AGCCAATGCC CAATGAATTC AATTTTTGGG ATTTCGAAAA GTACAACTTT 
GATTATTACA TTAAGGAAGA GAATCCGCTA CAGGAAATGC CTATGCCTAT TAAAGAAAAT 
AGAAGTAAAG AAAATGTTGA TGCTTCTTCA GTTGACTTGG AAAATCTTCA AAAGGATCAG 
GATGGCAAAA GGAGAGATCC AGACAAACTA CCAGTGGCCT TAGAAAATGT TGAAACCCAA 
AAAGAGTTGG AGCTCGAAAA CTTACCAGAA AGTTCACATA ATAATAAGAA TCTTAGGGTC 
AAAGAGAAAG AAATTGTAAG GCAAGGAAAG CTTAAAGAAA CTAATTCCAA AGCTGGAGGT 
TCTAAAACGA ACAAGACGGA GAGAAGGAAA AAATCTAACA CTCCATCAAA GTTACAGAAA 
CAAATAACTG AAGTGCAGCA AACACCAGAG ATCACCGTAA ATAAATACTA TATGCAAGAA 
GACATACAAC GAACAGCAGA CGGCAAAGAG TATCATCCTG TTAATGGAGC GGGAAACCAC 
CAGGTTCCGT CAAAATCTAA TCAAAAACCG GTGGAGTTTA TATCTCCCGA ATTGACACCA 
ACCCTAACAC CATTGGAACT GACCCAGGAG CCGTCTCCAA CCACAGTGAC ACCACCTGCA 
CCGCCAGCCA ACTCGATTAA CAACAAAACC TATTGCCTAG CTGCTCCAAC GCAAACCAGT 
TCGATGACCA TACAGAACAT GGTAATATCG AACACGGTGG ACGATGCCAG TAGCATCAGT 
TCGGATAGCA TGGTGGTATG TGGCTATCAT AGCGATAGGT CCTACGACAC ATACTATGCA 
ACCAGTGATG AGGAGGAGAT CTGGAATAGA TTAATGTCGA AACAAAGGCG TCAGCTGAAG 
AGCAAGAGAG GCCATGGAAA ACGGCGTGTG ATACTATACA AGAAAGTATT TCCTAAACTA 
AAGGCGAATG TTAAACGCAA GCTTAAAAAA CGGATGAGCT CGTCATGCAG ATCCAAAAAA 
GCACACCATT TGCGAAAACG TCATGTATAC AAAAATATAT CACTTTTGCC ACACATGAAT 
TTGTGTCATC TGCTTCAAAA ATTTACAAAT CAAGAGAGAT TTTTGAAACG GCTCCACAAA 
TATGCAAAGA ATCAATTCAT TGACAATGCG AGGGTTGGCT TTTTGCTGGA TAAGCTCTAT 
AAATCCATCC TGGAAATGTA CTATAACAGT GCTAAGGAGA GGAAGAAGCA TTTTAAGGAA 
GCTATGAAGG AGTCCCTAAA ATCCATTGAT AACTACGAGA GCCAAGAGTC TTCGGAATCA 
GAGGAGGAGG AGGAGGAGGA GAAGGATCTA AAGAACAAGT TAAGTGTTCA TGTGACGAAT 
CAGATGGACA TACCACCAAT GCCACAGGCC GCTGTTGAAA TTGGTTCAGG AAGTGAATCG 
GATACGGAGG ACTCGCAGGA ATACGGCGAA GGCGAAGAGA TCATTGTGGA CGATGAGGAC 
GATGGCAGCG ATGGCGGGCC AAATTCATCG AGTGGTTTTG TAGAGAGTCA GGAGAGTGTT 
TCGTCTGGAA TAGACACTTC CGTTTACGAA ACGGAATCTA CGGGCGCATC TGGAAATAAT 
TCAAGTGCAT TCTTTATTTC GGATGATGAA TCAGATACTG AATCCTTAAC AGATTCGGAA 
GCTGGAAGCA ATGTCAGCGC GACAGGATCA AGCTCACAGC GATCTTCAGT AGCGAGCAGC 
ACCTATGAAT CTTCGGATGA TGATTCTGTA ACTGTAACCA ATGTCAGTTC AATGCAGAAC 
GCTCAATCAT TGTGGTTGCA GACTTTCAAC ATGCAGTACC AATTCATTGC ATCCCATGTG 
GCCAAGAATC TACAGAACTA TTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gem3 ( NM_079295.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524019.1 Drosophila melanogaster Gemin3 (Gem3), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa18151
Clone ID Related Accession (Same CDS sequence)	NM_001274749.1 , NM_079295.4
Accession Version	NM_079295.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Gemin3, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079295.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCGCG AAATAGCGCA CAGTTTAGCT GGCGGCGAGG AACGAAGTAG CGATGTGGCA 
CCTGGACAGG TTAAGACCTT CGAGGAACTG CGTCTATACC GAAATCTGTT GAACGGATTG 
AAGCGCAACA ATTTCGTGAC GCCAACGAAG ATTCAGGCAG CTGCCATACC CATGGCACTG 
GCCAAAATGG ATCTAATAAT ACAATCGAAG AGTGGAACTG GCAAAACTCT AATCTATGTT 
ATAGCCGTCG TTCAGAGCTT TAATCCCAAC ATAAACCAAC CGCATGCCAT GATCGTGGTC 
CCAACTCGAG AGCTGGCCAT TCAGGTGCAG GACACATTTT TCCATCTCTG CAAATCGTTT 
CGGGACTTTA AATGCTCTGC TTTCATCGGT GGCACCGATG TGGCCAAGGA TCGCAAGCGA 
ATGAATGAAA GTCGAGTCAT TATCGGTACA CCGGGACGTT TGCTGCATCT GTATGAGAAT 
AGAGTATTCG ATGTGTCCAA ACTGCGACTG CTGGTGCTCG ACGAGGCCGA TCAACTTTAT 
CAGACAAAGA GCCTGCAGCA TACGGTCAGT AAACTCATTG AGGCAATGCC CAAGAATCGG 
CAAATAATTG CCTGCAGTGC CACATACGAT CAGAATTTGG ATGAGCGTCT GGCCAAGGTC 
ATGGACAAAC CCATGCTTAT ATCGAATTCC GAAAGGGCTA CCGTTCTACT GGGCATTCGG 
CAGTTTGTCT ACGAATTGCC ACAGCAGAAT AACAGTGTGG AGGAGATGCG TCTTAAACTG 
CAGATTCTTG GCCAGATCTT TAATCAGCTG CCTTACGAAC AGGCCATCAT CTTTGCTAGC 
TCACAAATGC GAGCGGATTC TTACAAGAAC TATCTTACAG CCAGTGGTAT CGATTGTCAT 
TTAATTTCGG GAGCCATGGA ACAATCGGAG CGTTTGCATG TTTTCGAGGG CTATAGAAAC 
TTTACTATGC GCATCCTGGT GGCCACTGAT TTGATGGCAC GCGGAGTGGA CTCCCCGCAT 
GCCAACCTGG TTATCAACAT TGATCCACCA CAGGATCATG TCACCTATTT GCACCGCATT 
GGACGAGCTG GTCGGTTTGG CTCCAAAGGC ATAGCCATCA CGTTTATTGC CTCCAAGAAG 
GAGAGCCAGA GATTTAGGGA AATGTCAAAA AAAATCGCTA CGGCTTGGTC GGTTCTGGAG 
TTTCCCAAGG AGCCAATGCC CAATGAATTC AATTTTTGGG ATTTCGAAAA GTACAACTTT 
GATTATTACA TTAAGGAAGA GAATCCGCTA CAGGAAATGC CTATGCCTAT TAAAGAAAAT 
AGAAGTAAAG AAAATGTTGA TGCTTCTTCA GTTGACTTGG AAAATCTTCA AAAGGATCAG 
GATGGCAAAA GGAGAGATCC AGACAAACTA CCAGTGGCCT TAGAAAATGT TGAAACCCAA 
AAAGAGTTGG AGCTCGAAAA CTTACCAGAA AGTTCACATA ATAATAAGAA TCTTAGGGTC 
AAAGAGAAAG AAATTGTAAG GCAAGGAAAG CTTAAAGAAA CTAATTCCAA AGCTGGAGGT 
TCTAAAACGA ACAAGACGGA GAGAAGGAAA AAATCTAACA CTCCATCAAA GTTACAGAAA 
CAAATAACTG AAGTGCAGCA AACACCAGAG ATCACCGTAA ATAAATACTA TATGCAAGAA 
GACATACAAC GAACAGCAGA CGGCAAAGAG TATCATCCTG TTAATGGAGC GGGAAACCAC 
CAGGTTCCGT CAAAATCTAA TCAAAAACCG GTGGAGTTTA TATCTCCCGA ATTGACACCA 
ACCCTAACAC CATTGGAACT GACCCAGGAG CCGTCTCCAA CCACAGTGAC ACCACCTGCA 
CCGCCAGCCA ACTCGATTAA CAACAAAACC TATTGCCTAG CTGCTCCAAC GCAAACCAGT 
TCGATGACCA TACAGAACAT GGTAATATCG AACACGGTGG ACGATGCCAG TAGCATCAGT 
TCGGATAGCA TGGTGGTATG TGGCTATCAT AGCGATAGGT CCTACGACAC ATACTATGCA 
ACCAGTGATG AGGAGGAGAT CTGGAATAGA TTAATGTCGA AACAAAGGCG TCAGCTGAAG 
AGCAAGAGAG GCCATGGAAA ACGGCGTGTG ATACTATACA AGAAAGTATT TCCTAAACTA 
AAGGCGAATG TTAAACGCAA GCTTAAAAAA CGGATGAGCT CGTCATGCAG ATCCAAAAAA 
GCACACCATT TGCGAAAACG TCATGTATAC AAAAATATAT CACTTTTGCC ACACATGAAT 
TTGTGTCATC TGCTTCAAAA ATTTACAAAT CAAGAGAGAT TTTTGAAACG GCTCCACAAA 
TATGCAAAGA ATCAATTCAT TGACAATGCG AGGGTTGGCT TTTTGCTGGA TAAGCTCTAT 
AAATCCATCC TGGAAATGTA CTATAACAGT GCTAAGGAGA GGAAGAAGCA TTTTAAGGAA 
GCTATGAAGG AGTCCCTAAA ATCCATTGAT AACTACGAGA GCCAAGAGTC TTCGGAATCA 
GAGGAGGAGG AGGAGGAGGA GAAGGATCTA AAGAACAAGT TAAGTGTTCA TGTGACGAAT 
CAGATGGACA TACCACCAAT GCCACAGGCC GCTGTTGAAA TTGGTTCAGG AAGTGAATCG 
GATACGGAGG ACTCGCAGGA ATACGGCGAA GGCGAAGAGA TCATTGTGGA CGATGAGGAC 
GATGGCAGCG ATGGCGGGCC AAATTCATCG AGTGGTTTTG TAGAGAGTCA GGAGAGTGTT 
TCGTCTGGAA TAGACACTTC CGTTTACGAA ACGGAATCTA CGGGCGCATC TGGAAATAAT 
TCAAGTGCAT TCTTTATTTC GGATGATGAA TCAGATACTG AATCCTTAAC AGATTCGGAA 
GCTGGAAGCA ATGTCAGCGC GACAGGATCA AGCTCACAGC GATCTTCAGT AGCGAGCAGC 
ACCTATGAAT CTTCGGATGA TGATTCTGTA ACTGTAACCA ATGTCAGTTC AATGCAGAAC 
GCTCAATCAT TGTGGTTGCA GACTTTCAAC ATGCAGTACC AATTCATTGC ATCCCATGTG 
GCCAAGAATC TACAGAACTA TTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524019.1
CDS	76..3162
Translation	MEREIAHSLAGGEERSSDVAPGQVKTFEELRLYRNLLNGLKRNNFVTPTKIQAAAIPMALAKMDLIIQSKSGTGKTLIYVIAVVQSFNPNINQPHAMIVVPTRELAIQVQDTFFHLCKSFRDFKCSAFIGGTDVAKDRKRMNESRVIIGTPGRLLHLYENRVFDVSKLRLLVLDEADQLYQTKSLQHTVSKLIEAMPKNRQIIACSATYDQNLDERLAKVMDKPMLISNSERATVLLGIRQFVYELPQQNNSVEEMRLKLQILGQIFNQLPYEQAIIFASSQMRADSYKNYLTASGIDCHLISGAMEQSERLHVFEGYRNFTMRILVATDLMARGVDSPHANLVINIDPPQDHVTYLHRIGRAGRFGSKGIAITFIASKKESQRFREMSKKIATAWSVLEFPKEPMPNEFNFWDFEKYNFDYYIKEENPLQEMPMPIKENRSKENVDASSVDLENLQKDQDGKRRDPDKLPVALENVETQKELELENLPESSHNNKNLRVKEKEIVRQGKLKETNSKAGGSKTNKTERRKKSNTPSKLQKQITEVQQTPEITVNKYYMQEDIQRTADGKEYHPVNGAGNHQVPSKSNQKPVEFISPELTPTLTPLELTQEPSPTTVTPPAPPANSINNKTYCLAAPTQTSSMTIQNMVISNTVDDASSISSDSMVVCGYHSDRSYDTYYATSDEEEIWNRLMSKQRRQLKSKRGHGKRRVILYKKVFPKLKANVKRKLKKRMSSSCRSKKAHHLRKRHVYKNISLLPHMNLCHLLQKFTNQERFLKRLHKYAKNQFIDNARVGFLLDKLYKSILEMYYNSAKERKKHFKEAMKESLKSIDNYESQESSESEEEEEEEKDLKNKLSVHVTNQMDIPPMPQAAVEIGSGSESDTEDSQEYGEGEEIIVDDEDDGSDGGPNSSSGFVESQESVSSGIDTSVYETESTGASGNNSSAFFISDDESDTESLTDSEAGSNVSATGSSSQRSSVASSTYESSDDDSVTVTNVSSMQNAQSLWLQTFNMQYQFIASHVAKNLQNYY

Target ORF information:

RefSeq Version	NM_079295.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Gemin3 (Gem3), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079295.4

ORF Insert Sequence:

ATGGAGCGCG AAATAGCGCA CAGTTTAGCT GGCGGCGAGG AACGAAGTAG CGATGTGGCA 
CCTGGACAGG TTAAGACCTT CGAGGAACTG CGTCTATACC GAAATCTGTT GAACGGATTG 
AAGCGCAACA ATTTCGTGAC GCCAACGAAG ATTCAGGCAG CTGCCATACC CATGGCACTG 
GCCAAAATGG ATCTAATAAT ACAATCGAAG AGTGGAACTG GCAAAACTCT AATCTATGTT 
ATAGCCGTCG TTCAGAGCTT TAATCCCAAC ATAAACCAAC CGCATGCCAT GATCGTGGTC 
CCAACTCGAG AGCTGGCCAT TCAGGTGCAG GACACATTTT TCCATCTCTG CAAATCGTTT 
CGGGACTTTA AATGCTCTGC TTTCATCGGT GGCACCGATG TGGCCAAGGA TCGCAAGCGA 
ATGAATGAAA GTCGAGTCAT TATCGGTACA CCGGGACGTT TGCTGCATCT GTATGAGAAT 
AGAGTATTCG ATGTGTCCAA ACTGCGACTG CTGGTGCTCG ACGAGGCCGA TCAACTTTAT 
CAGACAAAGA GCCTGCAGCA TACGGTCAGT AAACTCATTG AGGCAATGCC CAAGAATCGG 
CAAATAATTG CCTGCAGTGC CACATACGAT CAGAATTTGG ATGAGCGTCT GGCCAAGGTC 
ATGGACAAAC CCATGCTTAT ATCGAATTCC GAAAGGGCTA CCGTTCTACT GGGCATTCGG 
CAGTTTGTCT ACGAATTGCC ACAGCAGAAT AACAGTGTGG AGGAGATGCG TCTTAAACTG 
CAGATTCTTG GCCAGATCTT TAATCAGCTG CCTTACGAAC AGGCCATCAT CTTTGCTAGC 
TCACAAATGC GAGCGGATTC TTACAAGAAC TATCTTACAG CCAGTGGTAT CGATTGTCAT 
TTAATTTCGG GAGCCATGGA ACAATCGGAG CGTTTGCATG TTTTCGAGGG CTATAGAAAC 
TTTACTATGC GCATCCTGGT GGCCACTGAT TTGATGGCAC GCGGAGTGGA CTCCCCGCAT 
GCCAACCTGG TTATCAACAT TGATCCACCA CAGGATCATG TCACCTATTT GCACCGCATT 
GGACGAGCTG GTCGGTTTGG CTCCAAAGGC ATAGCCATCA CGTTTATTGC CTCCAAGAAG 
GAGAGCCAGA GATTTAGGGA AATGTCAAAA AAAATCGCTA CGGCTTGGTC GGTTCTGGAG 
TTTCCCAAGG AGCCAATGCC CAATGAATTC AATTTTTGGG ATTTCGAAAA GTACAACTTT 
GATTATTACA TTAAGGAAGA GAATCCGCTA CAGGAAATGC CTATGCCTAT TAAAGAAAAT 
AGAAGTAAAG AAAATGTTGA TGCTTCTTCA GTTGACTTGG AAAATCTTCA AAAGGATCAG 
GATGGCAAAA GGAGAGATCC AGACAAACTA CCAGTGGCCT TAGAAAATGT TGAAACCCAA 
AAAGAGTTGG AGCTCGAAAA CTTACCAGAA AGTTCACATA ATAATAAGAA TCTTAGGGTC 
AAAGAGAAAG AAATTGTAAG GCAAGGAAAG CTTAAAGAAA CTAATTCCAA AGCTGGAGGT 
TCTAAAACGA ACAAGACGGA GAGAAGGAAA AAATCTAACA CTCCATCAAA GTTACAGAAA 
CAAATAACTG AAGTGCAGCA AACACCAGAG ATCACCGTAA ATAAATACTA TATGCAAGAA 
GACATACAAC GAACAGCAGA CGGCAAAGAG TATCATCCTG TTAATGGAGC GGGAAACCAC 
CAGGTTCCGT CAAAATCTAA TCAAAAACCG GTGGAGTTTA TATCTCCCGA ATTGACACCA 
ACCCTAACAC CATTGGAACT GACCCAGGAG CCGTCTCCAA CCACAGTGAC ACCACCTGCA 
CCGCCAGCCA ACTCGATTAA CAACAAAACC TATTGCCTAG CTGCTCCAAC GCAAACCAGT 
TCGATGACCA TACAGAACAT GGTAATATCG AACACGGTGG ACGATGCCAG TAGCATCAGT 
TCGGATAGCA TGGTGGTATG TGGCTATCAT AGCGATAGGT CCTACGACAC ATACTATGCA 
ACCAGTGATG AGGAGGAGAT CTGGAATAGA TTAATGTCGA AACAAAGGCG TCAGCTGAAG 
AGCAAGAGAG GCCATGGAAA ACGGCGTGTG ATACTATACA AGAAAGTATT TCCTAAACTA 
AAGGCGAATG TTAAACGCAA GCTTAAAAAA CGGATGAGCT CGTCATGCAG ATCCAAAAAA 
GCACACCATT TGCGAAAACG TCATGTATAC AAAAATATAT CACTTTTGCC ACACATGAAT 
TTGTGTCATC TGCTTCAAAA ATTTACAAAT CAAGAGAGAT TTTTGAAACG GCTCCACAAA 
TATGCAAAGA ATCAATTCAT TGACAATGCG AGGGTTGGCT TTTTGCTGGA TAAGCTCTAT 
AAATCCATCC TGGAAATGTA CTATAACAGT GCTAAGGAGA GGAAGAAGCA TTTTAAGGAA 
GCTATGAAGG AGTCCCTAAA ATCCATTGAT AACTACGAGA GCCAAGAGTC TTCGGAATCA 
GAGGAGGAGG AGGAGGAGGA GAAGGATCTA AAGAACAAGT TAAGTGTTCA TGTGACGAAT 
CAGATGGACA TACCACCAAT GCCACAGGCC GCTGTTGAAA TTGGTTCAGG AAGTGAATCG 
GATACGGAGG ACTCGCAGGA ATACGGCGAA GGCGAAGAGA TCATTGTGGA CGATGAGGAC 
GATGGCAGCG ATGGCGGGCC AAATTCATCG AGTGGTTTTG TAGAGAGTCA GGAGAGTGTT 
TCGTCTGGAA TAGACACTTC CGTTTACGAA ACGGAATCTA CGGGCGCATC TGGAAATAAT 
TCAAGTGCAT TCTTTATTTC GGATGATGAA TCAGATACTG AATCCTTAAC AGATTCGGAA 
GCTGGAAGCA ATGTCAGCGC GACAGGATCA AGCTCACAGC GATCTTCAGT AGCGAGCAGC 
ACCTATGAAT CTTCGGATGA TGATTCTGTA ACTGTAACCA ATGTCAGTTC AATGCAGAAC 
GCTCAATCAT TGTGGTTGCA GACTTTCAAC ATGCAGTACC AATTCATTGC ATCCCATGTG 
GCCAAGAATC TACAGAACTA TTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Gem3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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