PGRP-LF cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	PGRP-LF
Entrez Gene ID	39064
Full Name	Peptidoglycan recognition protein LF
Synonyms	CG4437,Dmel\CG4437,PGRP-like,PGRPLF,anon-WO0118547.581,pgrp-lf
General protein information	Preferred Names Peptidoglycan recognition protein LF Names CG4437 gene product from transcript CG4437-RA CG4437-PA PGRP-LF-PA PRGP-like Peptidoglycan-recognition protein-LF peptidoglycan recognition protein peptidoglycan recognition protein LF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 67B1-67B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_140042.3	NP_648299.3	peptidoglycan recognition protein LF

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pglyrp3	NP_997130.2
	PGLYRP3	XP_001110242.1
Rattus norvegicus (Norway rat)	Pglyrp3	NP_001178890.1
Rattus norvegicus (Norway rat)	Pglyrp3b	XP_006232903.1
Drosophila melanogaster (fruit fly)	PGRP-LF	NP_648299.3
Bos taurus (cattle)	PGLYRP3	XP_002686050.3
Homo sapiens (human)	PGLYRP3	NP_443123.1
Pan troglodytes (chimpanzee)	PGLYRP3	XP_001137787.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Negative regulation of NF-kappaB/IMD pathway by PGRP-LF is crucial to ensure proper induction of apoptosis and consequently normal fly development.
Title: Inhibition of a NF-?B/Diap1 Pathway by PGRP-LF Is Required for Proper Apoptosis during Drosophila Development.

Data show that the Drosophila peptidoglycan-recognition protein LF interacts with peptidoglycan-recognition protein LC to downregulate the Imd pathway.
Title: The Drosophila peptidoglycan-recognition protein LF interacts with peptidoglycan-recognition protein LC to downregulate the Imd pathway.

By genetically interacting with PGRP-LC, PGRP-LF prevents constitutive activation of both the JNK and the immune deficiency pathways in the absence of infection.
Title: The Drosophila peptidoglycan recognition protein PGRP-LF blocks PGRP-LC and IMD/JNK pathway activation.

membrane bound and has its two PGRP domains, z and w, localized outside the cell
Title: Peptidoglycan recognition protein LF: a negative regulator of Drosophila immunity.

The following PGRP-LF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PGRP-LF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa18011	NM_140042.3 Latest version!	Drosophila melanogaster peptidoglycan recognition protein LF (PGRP-LF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PGRP-LF ( NM_140042.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648299.3 Drosophila melanogaster peptidoglycan recognition protein LF (PGRP-LF), mRNA.

Price & Availability↑

CloneID	OFa18011
Clone ID Related Accession (Same CDS sequence)	NM_140042.3
Accession Version	NM_140042.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	peptidoglycan recognition protein LF
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_140042.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGAAAA CAGTGGTAGT TACTCACCCA GGAAAACCGA TAAATAACGA GAAAAGATTT 
CGCTTCGAGC TTCTCTATTT CTGTGTGATT CTTCTAATGG TTGTGGGTTT AGCAGCTGGC 
TATTTCATGT GGATGATGTC CTTCTCCACA CATTCGCCGA ACAAGGGCCT ACACATACTC 
GATCGCAGCG AATGGCTGGG GGAACCACCC AGTGGGAAGT ACCCGCACCT TAAGCTTCCC 
GTCTCCAATA TTATCATTCA TCACACGGCA ACCGAAGGAT GCGAACAAGA GGATGTTTGC 
ATATACCGCA TGAAGACCAT TCAGGCGTTC CATATGAAAT CTTTTGGTTG GGTCGACATT 
GGCTATAACT TTTTGGTGGG CGGCGATGGT CAAATATACG TAGGACGCGG CTGGCACATC 
CAGGGTCAGC ATGTGAATGG ATATGGTGCC ATCTCCGTTA GCATAGCCTT TATCGGAACT 
TTTGTGAATA TGGAGCCACC AGCTCGACAA ATCGAAGCAG CCAAGCGACT GATGGACGAG 
GGCGTACGTC TCCATAGATT GCAACCGGAT TATCATATAT ATGCCCATCG ACAATTAAGC 
CCCACCGAAA GTCCTGGCCA GAAGCTTTTC GAGCTGATGC AAAATTGGCC CCGATTCACG 
CAGGATCCAA CATCATTGCG ACTCCTAAGC AATGAGACTG TAAAGATCGT TACCCGACCC 
TATTGGTTGG CCCAGCCACC TATAGTGCCA CTTACTCCTT TGAAGCTGCC AATTGAGAGC 
GTGAGATTTG TGGCAACCAA CACGCCATCA TGTTTCACTC AAGCCGAGTG CACTTTTCGA 
GTGCGCCTTC TGCAAAATTG GCACATCGAG AGCAATGGAT ATAAGGATAT TAACTATAAT 
TTCGTTGCGG CTGGGGACGA AAACATCTAC GAGGCCAGAG GTTGGGATCA CAGCTGTGAA 
CCTCCAAAAG ATGCAGATGA ACTAGTCGTG GCCTTTATTG GTCCATCGAG TAGCAATAAA 
AAGATAGCAT TAGAACTAAT CAAACAGGGC ATAAAACTTG GTCATATAAG CAAGAACTAT 
AGTCTAATTG ACGATTTAGA AAAGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648299.3
CDS	78..1187
Translation	MPKTVVVTHPGKPINNEKRFRFELLYFCVILLMVVGLAAGYFMWMMSFSTHSPNKGLHILDRSEWLGEPPSGKYPHLKLPVSNIIIHHTATEGCEQEDVCIYRMKTIQAFHMKSFGWVDIGYNFLVGGDGQIYVGRGWHIQGQHVNGYGAISVSIAFIGTFVNMEPPARQIEAAKRLMDEGVRLHRLQPDYHIYAHRQLSPTESPGQKLFELMQNWPRFTQDPTSLRLLSNETVKIVTRPYWLAQPPIVPLTPLKLPIESVRFVATNTPSCFTQAECTFRVRLLQNWHIESNGYKDINYNFVAAGDENIYEARGWDHSCEPPKDADELVVAFIGPSSSNKKIALELIKQGIKLGHISKNYSLIDDLEKS

Target ORF information:

RefSeq Version	NM_140042.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster peptidoglycan recognition protein LF (PGRP-LF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_140042.3

ORF Insert Sequence:

ATGCCGAAAA CAGTGGTAGT TACTCACCCA GGAAAACCGA TAAATAACGA GAAAAGATTT 
CGCTTCGAGC TTCTCTATTT CTGTGTGATT CTTCTAATGG TTGTGGGTTT AGCAGCTGGC 
TATTTCATGT GGATGATGTC CTTCTCCACA CATTCGCCGA ACAAGGGCCT ACACATACTC 
GATCGCAGCG AATGGCTGGG GGAACCACCC AGTGGGAAGT ACCCGCACCT TAAGCTTCCC 
GTCTCCAATA TTATCATTCA TCACACGGCA ACCGAAGGAT GCGAACAAGA GGATGTTTGC 
ATATACCGCA TGAAGACCAT TCAGGCGTTC CATATGAAAT CTTTTGGTTG GGTCGACATT 
GGCTATAACT TTTTGGTGGG CGGCGATGGT CAAATATACG TAGGACGCGG CTGGCACATC 
CAGGGTCAGC ATGTGAATGG ATATGGTGCC ATCTCCGTTA GCATAGCCTT TATCGGAACT 
TTTGTGAATA TGGAGCCACC AGCTCGACAA ATCGAAGCAG CCAAGCGACT GATGGACGAG 
GGCGTACGTC TCCATAGATT GCAACCGGAT TATCATATAT ATGCCCATCG ACAATTAAGC 
CCCACCGAAA GTCCTGGCCA GAAGCTTTTC GAGCTGATGC AAAATTGGCC CCGATTCACG 
CAGGATCCAA CATCATTGCG ACTCCTAAGC AATGAGACTG TAAAGATCGT TACCCGACCC 
TATTGGTTGG CCCAGCCACC TATAGTGCCA CTTACTCCTT TGAAGCTGCC AATTGAGAGC 
GTGAGATTTG TGGCAACCAA CACGCCATCA TGTTTCACTC AAGCCGAGTG CACTTTTCGA 
GTGCGCCTTC TGCAAAATTG GCACATCGAG AGCAATGGAT ATAAGGATAT TAACTATAAT 
TTCGTTGCGG CTGGGGACGA AAACATCTAC GAGGCCAGAG GTTGGGATCA CAGCTGTGAA 
CCTCCAAAAG ATGCAGATGA ACTAGTCGTG GCCTTTATTG GTCCATCGAG TAGCAATAAA 
AAGATAGCAT TAGAACTAAT CAAACAGGGC ATAAAACTTG GTCATATAAG CAAGAACTAT 
AGTCTAATTG ACGATTTAGA AAAGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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PGRP-LF cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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