Pus7 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pus7
Entrez Gene ID	38969
Full Name	CG6745 gene product from transcript CG6745-RA
Synonyms	CT20937,Dmel\CG6745
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66D5-66D5

mRNA and Protein(s)

mRNA	Protein	Name
NM_139974.4	NP_648231.1	pseudouridine synthase 7

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PUS7	XP_003318781.1
Rattus norvegicus (Norway rat)	Pus7	NP_001164060.1
Kluyveromyces lactis	KLLA0F11253g	XP_455592.1
Schizosaccharomyces pombe (fission yeast)	SPBC1A4.09	NP_595812.1
	PUS7	XP_001089755.2
Homo sapiens (human)	PUS7	NP_061915.2
Canis lupus familiaris (dog)	PUS7	XP_005631019.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005743	XP_556395.3
Saccharomyces cerevisiae (baker's yeast)	PUS7	NP_014886.1
Eremothecium gossypii	AGOS_ACR136C	NP_983538.1
Oryza sativa (rice)	Os05g0509300	NP_001174483.1
Mus musculus (house mouse)	Pus7	NP_848490.2
Gallus gallus (chicken)	PUS7	NP_001186468.1
Danio rerio (zebrafish)	pus7	NP_001116170.1
Arabidopsis thaliana (thale cress)	AT3G04820	NP_187133.3
Bos taurus (cattle)	PUS7	NP_001070407.1
Drosophila melanogaster (fruit fly)	CG6745	NP_648231.1
Xenopus tropicalis (tropical clawed frog)	pus7	NP_001016884.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pus7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pus7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa20737	NM_139974.4 Latest version!	Drosophila melanogaster pseudouridine synthase 7 (Pus7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pus7 ( NM_139974.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648231.1 Drosophila melanogaster pseudouridine synthase 7 (Pus7), mRNA.

Price & Availability↑

CloneID	OFa20737
Clone ID Related Accession (Same CDS sequence)	NM_139974.4
Accession Version	NM_139974.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pseudouridine synthase 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_139974.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCAAGG ATCGGGGCAG AGGACGTAGG AATAACCATT CCGGGCCATA CAACAAGAGC 
AACTGGCGTG GTCAGAAGAA GGATCGCCCC CAAAACAATG GTGGCGGTCA GCGCAACAGT 
GCTCCACAAC AGAAATCCAC GCTGCTGGAG ACCCAAGTGG GCATTACGGA GTTCACTAAC 
CCGGAGGCAC CTGGCTTCAC GGGAATACTT AAGTCTCGCT TCTCTGATTT TCACGTGAAC 
GAGATTGATA GCAATGGAAA GGTTCTGGAA CTGAACGATC TCAGTGTGCC TAAAGTGGCT 
ATTGTGGCTG TGGCCGAAAA CGAACTGGAC AACTGGCGGA AAGAACTGGA GGCAGTAATC 
GGCCCGGAAG TTTGGCAGGA TATAGCCAAT CTGGCTAAGG CCAAAAACGA TCCCGCGATC 
GAGCAAAAGG TGGAGATTGA TGTGACCACT CTGGATAAGG AGCAGCGAAC ACAAGTCCAC 
CAGATGATAA AACAGCTTTA CAAGGGCAAG CTACTGTCCA CAACCATTGG ACAGCAGCAG 
GCTAAGCCGA AGGTCCAGGA GGAGGCATCT TTGGAAAATA AGGATGCGGC TGAAGGTGAA 
ACCTCAACCC AGAAAGACTC ACAGGTGCCG GCAGAGGAGA AGAAGACTAT AAAAGTATTT 
AAACCAAAGC CAGGACGCGG TGACAAACGC TGGAGCTTTC CGGCGGATTA CGTGACCTTC 
CTGGTGCACA AAACCAATTT GGTTACCAGC GATGTGGCCT CTACTTTAGC AGCGCGACTG 
AACTTACGAC CATCGCAGGT GAACTACAGC GGCATCAAGG ACAAGCGGGC CAAAACCACC 
CAGAAGTTCT CCGTCAAGCG TCGTACACCA GAGAGCATTT TGGTTGCAGC TCGCTCCCAA 
CGGAATGTTC ATATCGGCAA CTTTGGATTC GAATCCAATA CACTGAAGCT AGGCGATTTG 
CAGGGCAATC GTTTTCGTAT TGCCTTGCGA CACATTGCCA AGGAGAAGCG AGAAGAAATC 
GAGCAGGCAC TGCAATCATT GAAGGAACGC GGCTTCATCA ACTACTATGG CTTGCAGCGG 
TTCGGCAACA GCGCCAGTGT CCCAACCTAT GAGGTGGGTG TTGCTCTGCT CAAGCACGAT 
TACAAACTGG CCTGCGAACT GATCCTGAAA CCCCGGGACT CGGATGTGGA GTTTTTGCGT 
CCCATACGCG AGGAGTGGTG GAAAAATCGC GACTCCGCGG CGGCAGCTGC ACAGATCTAC 
GGCGAAAAGT TCATCGAAAA GAAGCTGCTG GACGGCTTGG CCAGATTTGG TGAATCAGAT 
TACTCATCAG CTCTGCGGCA GATACCACGC AACATGCTCA TGCTATATCC ACATGCTTAC 
CAAAGCCTGA TCTTCAACAG GATAGCGTCT AGACGAATTA AGGAGTTCGG CTTGAAATTG 
ATACCCGGGG ATTTGGTTTA TGTGGAGCAG GATCAGTCGG AAGCCTCGGA AGAGCAGGCG 
CAGCAGGGCG AGGCCTTGGA AGAGCAAAAA GAAGAGGAGC CTAACGATGA ACCTCTGGAA 
GTCCCCGAAG GGGATGAAGC CATCGAAGAG GAGTCGATTT TCAAGCGAAA GGTTCGCCCG 
CTCACAGCAG AAGACATCGC TAGCGGCAAG TACAAACTTT GCGATGTGGT CCTTCCCCTG 
CCTGGACACG ACATCACCTA TCCCAGCAAC GAGTGCGGCG CGTGGTACGA GGAAATGCTC 
GCCGAAGTGG GTCTTTCCTC GGATCAACTG AAGCACAAGG AGAAGACATA CGCATTGGCT 
GGCGCCTACC GCAAGATGAT CATATCGTCC AGTGATCTCA AGTGGAGCTT TAGAATGTAC 
AATACGCCGG AGGATACACT TATCGCCTCC GACTGGGAGC TATTGAAGAA CATTCCGGTT 
GCACCAGAAC CAGCTGAAGC TGAGGCCAAT TACATGGCAC TCCTGCTGGA GTTCTCGCTG 
CCCACGGCTG CCTATGCCAC CATGTTTTTG CGAGAACTCT TAAAGCAGGA CACATCGTCA 
GCATCGCAAA CGCAGTTGGA GAAACAGGCA ATGGCTAAGG AGGACAATGA AAAGAAGGCG 
GTAGGAACCC AGGAGGAACA AATGGAGGTG GTAAAGGAGG TGGCCGACGA GGATGGGCAG 
GAAACTGAGC AGGCGGAAAC TCAGGGGGCC GAGGAGGCGG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648231.1
CDS	83..2287
Translation	MGKDRGRGRRNNHSGPYNKSNWRGQKKDRPQNNGGGQRNSAPQQKSTLLETQVGITEFTNPEAPGFTGILKSRFSDFHVNEIDSNGKVLELNDLSVPKVAIVAVAENELDNWRKELEAVIGPEVWQDIANLAKAKNDPAIEQKVEIDVTTLDKEQRTQVHQMIKQLYKGKLLSTTIGQQQAKPKVQEEASLENKDAAEGETSTQKDSQVPAEEKKTIKVFKPKPGRGDKRWSFPADYVTFLVHKTNLVTSDVASTLAARLNLRPSQVNYSGIKDKRAKTTQKFSVKRRTPESILVAARSQRNVHIGNFGFESNTLKLGDLQGNRFRIALRHIAKEKREEIEQALQSLKERGFINYYGLQRFGNSASVPTYEVGVALLKHDYKLACELILKPRDSDVEFLRPIREEWWKNRDSAAAAAQIYGEKFIEKKLLDGLARFGESDYSSALRQIPRNMLMLYPHAYQSLIFNRIASRRIKEFGLKLIPGDLVYVEQDQSEASEEQAQQGEALEEQKEEEPNDEPLEVPEGDEAIEEESIFKRKVRPLTAEDIASGKYKLCDVVLPLPGHDITYPSNECGAWYEEMLAEVGLSSDQLKHKEKTYALAGAYRKMIISSSDLKWSFRMYNTPEDTLIASDWELLKNIPVAPEPAEAEANYMALLLEFSLPTAAYATMFLRELLKQDTSSASQTQLEKQAMAKEDNEKKAVGTQEEQMEVVKEVADEDGQETEQAETQGAEEAV

Target ORF information:

RefSeq Version	NM_139974.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pseudouridine synthase 7 (Pus7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139974.4

ORF Insert Sequence:

ATGGGCAAGG ATCGGGGCAG AGGACGTAGG AATAACCATT CCGGGCCATA CAACAAGAGC 
AACTGGCGTG GTCAGAAGAA GGATCGCCCC CAAAACAATG GTGGCGGTCA GCGCAACAGT 
GCTCCACAAC AGAAATCCAC GCTGCTGGAG ACCCAAGTGG GCATTACGGA GTTCACTAAC 
CCGGAGGCAC CTGGCTTCAC GGGAATACTT AAGTCTCGCT TCTCTGATTT TCACGTGAAC 
GAGATTGATA GCAATGGAAA GGTTCTGGAA CTGAACGATC TCAGTGTGCC TAAAGTGGCT 
ATTGTGGCTG TGGCCGAAAA CGAACTGGAC AACTGGCGGA AAGAACTGGA GGCAGTAATC 
GGCCCGGAAG TTTGGCAGGA TATAGCCAAT CTGGCTAAGG CCAAAAACGA TCCCGCGATC 
GAGCAAAAGG TGGAGATTGA TGTGACCACT CTGGATAAGG AGCAGCGAAC ACAAGTCCAC 
CAGATGATAA AACAGCTTTA CAAGGGCAAG CTACTGTCCA CAACCATTGG ACAGCAGCAG 
GCTAAGCCGA AGGTCCAGGA GGAGGCATCT TTGGAAAATA AGGATGCGGC TGAAGGTGAA 
ACCTCAACCC AGAAAGACTC ACAGGTGCCG GCAGAGGAGA AGAAGACTAT AAAAGTATTT 
AAACCAAAGC CAGGACGCGG TGACAAACGC TGGAGCTTTC CGGCGGATTA CGTGACCTTC 
CTGGTGCACA AAACCAATTT GGTTACCAGC GATGTGGCCT CTACTTTAGC AGCGCGACTG 
AACTTACGAC CATCGCAGGT GAACTACAGC GGCATCAAGG ACAAGCGGGC CAAAACCACC 
CAGAAGTTCT CCGTCAAGCG TCGTACACCA GAGAGCATTT TGGTTGCAGC TCGCTCCCAA 
CGGAATGTTC ATATCGGCAA CTTTGGATTC GAATCCAATA CACTGAAGCT AGGCGATTTG 
CAGGGCAATC GTTTTCGTAT TGCCTTGCGA CACATTGCCA AGGAGAAGCG AGAAGAAATC 
GAGCAGGCAC TGCAATCATT GAAGGAACGC GGCTTCATCA ACTACTATGG CTTGCAGCGG 
TTCGGCAACA GCGCCAGTGT CCCAACCTAT GAGGTGGGTG TTGCTCTGCT CAAGCACGAT 
TACAAACTGG CCTGCGAACT GATCCTGAAA CCCCGGGACT CGGATGTGGA GTTTTTGCGT 
CCCATACGCG AGGAGTGGTG GAAAAATCGC GACTCCGCGG CGGCAGCTGC ACAGATCTAC 
GGCGAAAAGT TCATCGAAAA GAAGCTGCTG GACGGCTTGG CCAGATTTGG TGAATCAGAT 
TACTCATCAG CTCTGCGGCA GATACCACGC AACATGCTCA TGCTATATCC ACATGCTTAC 
CAAAGCCTGA TCTTCAACAG GATAGCGTCT AGACGAATTA AGGAGTTCGG CTTGAAATTG 
ATACCCGGGG ATTTGGTTTA TGTGGAGCAG GATCAGTCGG AAGCCTCGGA AGAGCAGGCG 
CAGCAGGGCG AGGCCTTGGA AGAGCAAAAA GAAGAGGAGC CTAACGATGA ACCTCTGGAA 
GTCCCCGAAG GGGATGAAGC CATCGAAGAG GAGTCGATTT TCAAGCGAAA GGTTCGCCCG 
CTCACAGCAG AAGACATCGC TAGCGGCAAG TACAAACTTT GCGATGTGGT CCTTCCCCTG 
CCTGGACACG ACATCACCTA TCCCAGCAAC GAGTGCGGCG CGTGGTACGA GGAAATGCTC 
GCCGAAGTGG GTCTTTCCTC GGATCAACTG AAGCACAAGG AGAAGACATA CGCATTGGCT 
GGCGCCTACC GCAAGATGAT CATATCGTCC AGTGATCTCA AGTGGAGCTT TAGAATGTAC 
AATACGCCGG AGGATACACT TATCGCCTCC GACTGGGAGC TATTGAAGAA CATTCCGGTT 
GCACCAGAAC CAGCTGAAGC TGAGGCCAAT TACATGGCAC TCCTGCTGGA GTTCTCGCTG 
CCCACGGCTG CCTATGCCAC CATGTTTTTG CGAGAACTCT TAAAGCAGGA CACATCGTCA 
GCATCGCAAA CGCAGTTGGA GAAACAGGCA ATGGCTAAGG AGGACAATGA AAAGAAGGCG 
GTAGGAACCC AGGAGGAACA AATGGAGGTG GTAAAGGAGG TGGCCGACGA GGATGGGCAG 
GAAACTGAGC AGGCGGAAAC TCAGGGGGCC GAGGAGGCGG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pus7 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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