tow cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	tow
Entrez Gene ID	38755
Full Name	target of wingless
Synonyms	CG14821,Dmel\CG14821,Tow
General protein information	Preferred Names target of wingless Names CG14821 gene product from transcript CG14821-RD CG14821-PC CG14821-PD CG14821-PE CG14821-PF CG14821-PG tow-PC tow-PD tow-PE tow-PF tow-PG
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 65D3-65D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274559.1	NP_001261488.1	target of wingless, isoform D
NM_001104056.2	NP_001097526.1	target of wingless, isoform C
NM_001274560.1	NP_001261489.1	target of wingless, isoform E
NM_139804.2	NP_648061.2	target of wingless, isoform F
NM_001274561.1	NP_001261490.1	target of wingless, isoform G

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies indicate that negative regulation of the Vestigial gradient by Wingless signaling makes vestigial expression precise and robust.
Title: Spatiotemporally modulated Vestigial gradient by Wingless signaling adaptively regulates cell division for precise wing size control.

results demonstrate that Tow is an important novel regulator of the Hh pathway in the wing imaginal disc, and may shed light on the mechanism of Ptc repression of Smo
Title: Tow (Target of Wingless), a novel repressor of the Hedgehog pathway in Drosophila.

Target of Wingless (tow) acts downstream of Fz/Dsh and plays a role in restricting the number of hairs that wing cells form.
Title: The balance between the novel protein target of wingless and the Drosophila Rho-associated kinase pathway regulates planar cell polarity in the Drosophila wing.

The following tow gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tow cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17729	NM_139804.2 Latest version!	Drosophila melanogaster target of wingless (tow), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OFa17730	NM_001274559.1 Latest version!	Drosophila melanogaster target of wingless (tow), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OFa17730	NM_001104056.2 Latest version!	Drosophila melanogaster target of wingless (tow), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OFa20632	NM_001274561.1 Latest version!	Drosophila melanogaster target of wingless (tow), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OFa20633	NM_001274560.1 Latest version!	Drosophila melanogaster target of wingless (tow), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tow ( NM_139804.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_648061.2 Drosophila melanogaster target of wingless (tow), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa17729
Clone ID Related Accession (Same CDS sequence)	NM_139804.2
Accession Version	NM_139804.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	target of wingless, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_139804.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCTGTG GACAGAGCAA GATACATTTG TATCCCAGAA AATCGAAGAG TAAAGCGAAC 
GGCAAGAAAG GAGGACATGC CGACTCAGAT GCCGAAACGG ACGAGGACGA AGGCCATATC 
GAGGATGCTG AGAAGGCACA GCAGCGCGAC CGCGAGAAGC ACGACGAGAG CGAGGAGCTA 
AGCAATAAGG ACATCCAGGA AAGCGACGAG GACGTGGCCG TCTCACTGCT GCGCGCCAAG 
AACCTATCGC TGCTGCAAAG CCACCAGCAG AACTTCTTCC GCATGCTGGA CAAGAAGATT 
GATGAGGGTC CCGACTACGA TTCGGCCAGC GAGACGGAGA TCGCATTGGA GGAGGCCCGA 
CTAAACGCCC TGCGTCAACA TTGGGAGTCC GCCAGCATTA CGGCCTCCAT CTGCTCCTCG 
GCCAGCCGCT CGCTGCAGAC GACGCCCATC CGCCAGGTGC AGGTGCCATT GAAGCAGCCA 
CCGCGTGGCG CCGGCCTGCT GATTCAGCCT TCTAGCGCCT CGGCGGTCAC CGCCTCCACC 
TCCTCCTCCT CCTCATCGAC GATAGTCGCG CTGCCCACGA CGGAGTATCT ACCTCAGCAT 
GTGTTGACGG GACGGCAAGT GGTGCAGCAG CAGCAACAAC AGCAGCAGCA ACAGGCGGCA 
GTGCTCTACC AGCAGCAGCA GCAGCAACTA ATACTGCTGC CCCAGTTGGA TCCTAATGTT 
GTCAACACGT CCACGGCAGC AGCAGCGGCA CAGCTCGTCC AGTTGCAGCA GCAGCATCAA 
CAGCAGCAAC AACAGCTACA GCTGCAGCAG CAACAGCAGC TGCAACTCAA TCAGCAGCAG 
CAGCAGCAAC TTTACCAGCA GCAGCAACAG CAATACCTGC TGACCCTTCC CGCCGGATCA 
AAGCCCCAGA GCGTGAGTCC AAAGCGCCTG ATCTATGGTG GACCCGCTGC AACCACTCTC 
CTCTGTGCAA CTGGCATCAA ATGCAACGCC CCCGAAACTT ACGAACCGCC AACCGCTCCA 
CACAGCCAAA TCCAGCAGCA ACAGCCACCG CCTCCTCCGC CGGGCGCCTA CTACGGCGAG 
ATGATGCACG GCGTACAGGC CGCTCCAGCG GCGGAATACA AGTTCCCGCC GGCCATCAGC 
GTGCAGAGAC TGGCGCCGCA GGTGCAGCGG CAGTTGCGCG AGACGCAGGA ACTCATAAAA 
GACTCGTGCC CGCAGCTGTA TGCGGCGGGC TATGGCAGCC CAGGACCACC GATACGCAAT 
CCCAGCAGGA ACCCCACTAG AACTAGACCC ACCCTGGAGA CGCAGTTCTC GCAGGAACTC 
TCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_648061.2
CDS	506..1831
Translation	MGCGQSKIHLYPRKSKSKANGKKGGHADSDAETDEDEGHIEDAEKAQQRDREKHDESEELSNKDIQESDEDVAVSLLRAKNLSLLQSHQQNFFRMLDKKIDEGPDYDSASETEIALEEARLNALRQHWESASITASICSSASRSLQTTPIRQVQVPLKQPPRGAGLLIQPSSASAVTASTSSSSSSTIVALPTTEYLPQHVLTGRQVVQQQQQQQQQQAAVLYQQQQQQLILLPQLDPNVVNTSTAAAAAQLVQLQQQHQQQQQQLQLQQQQQLQLNQQQQQQLYQQQQQQYLLTLPAGSKPQSVSPKRLIYGGPAATTLLCATGIKCNAPETYEPPTAPHSQIQQQQPPPPPPGAYYGEMMHGVQAAPAAEYKFPPAISVQRLAPQVQRQLRETQELIKDSCPQLYAAGYGSPGPPIRNPSRNPTRTRPTLETQFSQELS

Target ORF information:

RefSeq Version	NM_139804.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster target of wingless (tow), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139804.2

ORF Insert Sequence:

ATGGGCTGTG GACAGAGCAA GATACATTTG TATCCCAGAA AATCGAAGAG TAAAGCGAAC 
GGCAAGAAAG GAGGACATGC CGACTCAGAT GCCGAAACGG ACGAGGACGA AGGCCATATC 
GAGGATGCTG AGAAGGCACA GCAGCGCGAC CGCGAGAAGC ACGACGAGAG CGAGGAGCTA 
AGCAATAAGG ACATCCAGGA AAGCGACGAG GACGTGGCCG TCTCACTGCT GCGCGCCAAG 
AACCTATCGC TGCTGCAAAG CCACCAGCAG AACTTCTTCC GCATGCTGGA CAAGAAGATT 
GATGAGGGTC CCGACTACGA TTCGGCCAGC GAGACGGAGA TCGCATTGGA GGAGGCCCGA 
CTAAACGCCC TGCGTCAACA TTGGGAGTCC GCCAGCATTA CGGCCTCCAT CTGCTCCTCG 
GCCAGCCGCT CGCTGCAGAC GACGCCCATC CGCCAGGTGC AGGTGCCATT GAAGCAGCCA 
CCGCGTGGCG CCGGCCTGCT GATTCAGCCT TCTAGCGCCT CGGCGGTCAC CGCCTCCACC 
TCCTCCTCCT CCTCATCGAC GATAGTCGCG CTGCCCACGA CGGAGTATCT ACCTCAGCAT 
GTGTTGACGG GACGGCAAGT GGTGCAGCAG CAGCAACAAC AGCAGCAGCA ACAGGCGGCA 
GTGCTCTACC AGCAGCAGCA GCAGCAACTA ATACTGCTGC CCCAGTTGGA TCCTAATGTT 
GTCAACACGT CCACGGCAGC AGCAGCGGCA CAGCTCGTCC AGTTGCAGCA GCAGCATCAA 
CAGCAGCAAC AACAGCTACA GCTGCAGCAG CAACAGCAGC TGCAACTCAA TCAGCAGCAG 
CAGCAGCAAC TTTACCAGCA GCAGCAACAG CAATACCTGC TGACCCTTCC CGCCGGATCA 
AAGCCCCAGA GCGTGAGTCC AAAGCGCCTG ATCTATGGTG GACCCGCTGC AACCACTCTC 
CTCTGTGCAA CTGGCATCAA ATGCAACGCC CCCGAAACTT ACGAACCGCC AACCGCTCCA 
CACAGCCAAA TCCAGCAGCA ACAGCCACCG CCTCCTCCGC CGGGCGCCTA CTACGGCGAG 
ATGATGCACG GCGTACAGGC CGCTCCAGCG GCGGAATACA AGTTCCCGCC GGCCATCAGC 
GTGCAGAGAC TGGCGCCGCA GGTGCAGCGG CAGTTGCGCG AGACGCAGGA ACTCATAAAA 
GACTCGTGCC CGCAGCTGTA TGCGGCGGGC TATGGCAGCC CAGGACCACC GATACGCAAT 
CCCAGCAGGA ACCCCACTAG AACTAGACCC ACCCTGGAGA CGCAGTTCTC GCAGGAACTC 
TCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tow ( NM_001274559.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261488.1 Drosophila melanogaster target of wingless (tow), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa17730
Clone ID Related Accession (Same CDS sequence)	NM_001274559.1 , NM_001104056.2
Accession Version	NM_001274559.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	target of wingless, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCTGTG GACAGAGCAA GATACATTTG TATCCCAGAA AATCGAAGAG TAAAGCGAAC 
GGCAAGAAAG GAGGACATGC CGACTCAGAT GCCGAAACGG ACGAGGACGA AGGCCATATC 
GAGGATGCTG AGAAGGCACA GCAGCGCGAC CGCGAGAAGC ACGACGAGAG CGAGGAGCTA 
AGCAATAAGG ACATCCAGGA AAGCGACGAG GACGTGGCCG TCTCACTGCT GCGCGCCAAG 
AACCTATCGC TGCTGCAAAG CCAGGAAATA TCATCTAGCC AGCAGAACTT CTTCCGCATG 
CTGGACAAGA AGATTGATGA GGGTCCCGAC TACGATTCGG CCAGCGAGAC GGAGATCGCA 
TTGGAGGAGG CCCGACTAAA CGCCCTGCGT CAACATTGGG AGTCCGCCAG CATTACGGCC 
TCCATCTGCT CCTCGGCCAG CCGCTCGCTG CAGACGACGC CCATCCGCCA GGTGCAGGTG 
CCATTGAAGC AGCCACCGCG TGGCGCCGGC CTGCTGATTC AGCCTTCTAG CGCCTCGGCG 
GTCACCGCCT CCACCTCCTC CTCCTCCTCA TCGACGATAG TCGCGCTGCC CACGACGGAG 
TATCTACCTC AGCATGTGTT GACGGGACGG CAAGTGGTGC AGCAGCAGCA ACAACAGCAG 
CAGCAACAGG CGGCAGTGCT CTACCAGCAG CAGCAGCAGC AACTAATACT GCTGCCCCAG 
TTGGATCCTA ATGTTGTCAA CACGTCCACG GCAGCAGCAG CGGCACAGCT CGTCCAGTTG 
CAGCAGCAGC ATCAACAGCA GCAACAACAG CTACAGCTGC AGCAGCAACA GCAGCTGCAA 
CTCAATCAGC AGCAGCAGCA GCAACTTTAC CAGCAGCAGC AACAGCAATA CCTGCTGACC 
CTTCCCGCCG GATCAAAGCC CCAGAGCGTG AGTCCAAAGC GCCTGATCTA TGGTGGACCC 
GCTGCAACCA CTCTCCTCTG TGCAACTGGC ATCAAATGCA ACGCCCCCGA AACTTACGAA 
CCGCCAACCG CTCCACACAG CCAAATCCAG CAGCAACAGC CACCGCCTCC TCCGCCGGGC 
GCCTACTACG GCGAGATGAT GCACGGCGTA CAGGCCGCTC CAGCGGCGGA ATACAAGTTC 
CCGCCGGCCA TCAGCGTGCA GAGACTGGCG CCGCAGGTGC AGCGGCAGTT GCGCGAGACG 
CAGGAACTCA TAAAAGACTC GTGCCCGCAG CTGTATGCGG CGGGCTATGG CAGCCCAGGA 
CCACCGATAC GCAATCCCAG CAGGAACCCC ACTAGAACTA GACCCACCCT GGAGACGCAG 
TTCTCGCAGG AACTCTCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261488.1
CDS	506..1846
Translation	MGCGQSKIHLYPRKSKSKANGKKGGHADSDAETDEDEGHIEDAEKAQQRDREKHDESEELSNKDIQESDEDVAVSLLRAKNLSLLQSQEISSSQQNFFRMLDKKIDEGPDYDSASETEIALEEARLNALRQHWESASITASICSSASRSLQTTPIRQVQVPLKQPPRGAGLLIQPSSASAVTASTSSSSSSTIVALPTTEYLPQHVLTGRQVVQQQQQQQQQQAAVLYQQQQQQLILLPQLDPNVVNTSTAAAAAQLVQLQQQHQQQQQQLQLQQQQQLQLNQQQQQQLYQQQQQQYLLTLPAGSKPQSVSPKRLIYGGPAATTLLCATGIKCNAPETYEPPTAPHSQIQQQQPPPPPPGAYYGEMMHGVQAAPAAEYKFPPAISVQRLAPQVQRQLRETQELIKDSCPQLYAAGYGSPGPPIRNPSRNPTRTRPTLETQFSQELS

Target ORF information:

RefSeq Version	NM_001274559.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster target of wingless (tow), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274559.1

ORF Insert Sequence:

ATGGGCTGTG GACAGAGCAA GATACATTTG TATCCCAGAA AATCGAAGAG TAAAGCGAAC 
GGCAAGAAAG GAGGACATGC CGACTCAGAT GCCGAAACGG ACGAGGACGA AGGCCATATC 
GAGGATGCTG AGAAGGCACA GCAGCGCGAC CGCGAGAAGC ACGACGAGAG CGAGGAGCTA 
AGCAATAAGG ACATCCAGGA AAGCGACGAG GACGTGGCCG TCTCACTGCT GCGCGCCAAG 
AACCTATCGC TGCTGCAAAG CCAGGAAATA TCATCTAGCC AGCAGAACTT CTTCCGCATG 
CTGGACAAGA AGATTGATGA GGGTCCCGAC TACGATTCGG CCAGCGAGAC GGAGATCGCA 
TTGGAGGAGG CCCGACTAAA CGCCCTGCGT CAACATTGGG AGTCCGCCAG CATTACGGCC 
TCCATCTGCT CCTCGGCCAG CCGCTCGCTG CAGACGACGC CCATCCGCCA GGTGCAGGTG 
CCATTGAAGC AGCCACCGCG TGGCGCCGGC CTGCTGATTC AGCCTTCTAG CGCCTCGGCG 
GTCACCGCCT CCACCTCCTC CTCCTCCTCA TCGACGATAG TCGCGCTGCC CACGACGGAG 
TATCTACCTC AGCATGTGTT GACGGGACGG CAAGTGGTGC AGCAGCAGCA ACAACAGCAG 
CAGCAACAGG CGGCAGTGCT CTACCAGCAG CAGCAGCAGC AACTAATACT GCTGCCCCAG 
TTGGATCCTA ATGTTGTCAA CACGTCCACG GCAGCAGCAG CGGCACAGCT CGTCCAGTTG 
CAGCAGCAGC ATCAACAGCA GCAACAACAG CTACAGCTGC AGCAGCAACA GCAGCTGCAA 
CTCAATCAGC AGCAGCAGCA GCAACTTTAC CAGCAGCAGC AACAGCAATA CCTGCTGACC 
CTTCCCGCCG GATCAAAGCC CCAGAGCGTG AGTCCAAAGC GCCTGATCTA TGGTGGACCC 
GCTGCAACCA CTCTCCTCTG TGCAACTGGC ATCAAATGCA ACGCCCCCGA AACTTACGAA 
CCGCCAACCG CTCCACACAG CCAAATCCAG CAGCAACAGC CACCGCCTCC TCCGCCGGGC 
GCCTACTACG GCGAGATGAT GCACGGCGTA CAGGCCGCTC CAGCGGCGGA ATACAAGTTC 
CCGCCGGCCA TCAGCGTGCA GAGACTGGCG CCGCAGGTGC AGCGGCAGTT GCGCGAGACG 
CAGGAACTCA TAAAAGACTC GTGCCCGCAG CTGTATGCGG CGGGCTATGG CAGCCCAGGA 
CCACCGATAC GCAATCCCAG CAGGAACCCC ACTAGAACTA GACCCACCCT GGAGACGCAG 
TTCTCGCAGG AACTCTCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tow ( NM_001104056.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097526.1 Drosophila melanogaster target of wingless (tow), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa17730
Clone ID Related Accession (Same CDS sequence)	NM_001274559.1 , NM_001104056.2
Accession Version	NM_001104056.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	target of wingless, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001104056.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCTGTG GACAGAGCAA GATACATTTG TATCCCAGAA AATCGAAGAG TAAAGCGAAC 
GGCAAGAAAG GAGGACATGC CGACTCAGAT GCCGAAACGG ACGAGGACGA AGGCCATATC 
GAGGATGCTG AGAAGGCACA GCAGCGCGAC CGCGAGAAGC ACGACGAGAG CGAGGAGCTA 
AGCAATAAGG ACATCCAGGA AAGCGACGAG GACGTGGCCG TCTCACTGCT GCGCGCCAAG 
AACCTATCGC TGCTGCAAAG CCAGGAAATA TCATCTAGCC AGCAGAACTT CTTCCGCATG 
CTGGACAAGA AGATTGATGA GGGTCCCGAC TACGATTCGG CCAGCGAGAC GGAGATCGCA 
TTGGAGGAGG CCCGACTAAA CGCCCTGCGT CAACATTGGG AGTCCGCCAG CATTACGGCC 
TCCATCTGCT CCTCGGCCAG CCGCTCGCTG CAGACGACGC CCATCCGCCA GGTGCAGGTG 
CCATTGAAGC AGCCACCGCG TGGCGCCGGC CTGCTGATTC AGCCTTCTAG CGCCTCGGCG 
GTCACCGCCT CCACCTCCTC CTCCTCCTCA TCGACGATAG TCGCGCTGCC CACGACGGAG 
TATCTACCTC AGCATGTGTT GACGGGACGG CAAGTGGTGC AGCAGCAGCA ACAACAGCAG 
CAGCAACAGG CGGCAGTGCT CTACCAGCAG CAGCAGCAGC AACTAATACT GCTGCCCCAG 
TTGGATCCTA ATGTTGTCAA CACGTCCACG GCAGCAGCAG CGGCACAGCT CGTCCAGTTG 
CAGCAGCAGC ATCAACAGCA GCAACAACAG CTACAGCTGC AGCAGCAACA GCAGCTGCAA 
CTCAATCAGC AGCAGCAGCA GCAACTTTAC CAGCAGCAGC AACAGCAATA CCTGCTGACC 
CTTCCCGCCG GATCAAAGCC CCAGAGCGTG AGTCCAAAGC GCCTGATCTA TGGTGGACCC 
GCTGCAACCA CTCTCCTCTG TGCAACTGGC ATCAAATGCA ACGCCCCCGA AACTTACGAA 
CCGCCAACCG CTCCACACAG CCAAATCCAG CAGCAACAGC CACCGCCTCC TCCGCCGGGC 
GCCTACTACG GCGAGATGAT GCACGGCGTA CAGGCCGCTC CAGCGGCGGA ATACAAGTTC 
CCGCCGGCCA TCAGCGTGCA GAGACTGGCG CCGCAGGTGC AGCGGCAGTT GCGCGAGACG 
CAGGAACTCA TAAAAGACTC GTGCCCGCAG CTGTATGCGG CGGGCTATGG CAGCCCAGGA 
CCACCGATAC GCAATCCCAG CAGGAACCCC ACTAGAACTA GACCCACCCT GGAGACGCAG 
TTCTCGCAGG AACTCTCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097526.1
CDS	11..1351
Translation	MGCGQSKIHLYPRKSKSKANGKKGGHADSDAETDEDEGHIEDAEKAQQRDREKHDESEELSNKDIQESDEDVAVSLLRAKNLSLLQSQEISSSQQNFFRMLDKKIDEGPDYDSASETEIALEEARLNALRQHWESASITASICSSASRSLQTTPIRQVQVPLKQPPRGAGLLIQPSSASAVTASTSSSSSSTIVALPTTEYLPQHVLTGRQVVQQQQQQQQQQAAVLYQQQQQQLILLPQLDPNVVNTSTAAAAAQLVQLQQQHQQQQQQLQLQQQQQLQLNQQQQQQLYQQQQQQYLLTLPAGSKPQSVSPKRLIYGGPAATTLLCATGIKCNAPETYEPPTAPHSQIQQQQPPPPPPGAYYGEMMHGVQAAPAAEYKFPPAISVQRLAPQVQRQLRETQELIKDSCPQLYAAGYGSPGPPIRNPSRNPTRTRPTLETQFSQELS

Target ORF information:

RefSeq Version	NM_001104056.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster target of wingless (tow), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104056.2

ORF Insert Sequence:

ATGGGCTGTG GACAGAGCAA GATACATTTG TATCCCAGAA AATCGAAGAG TAAAGCGAAC 
GGCAAGAAAG GAGGACATGC CGACTCAGAT GCCGAAACGG ACGAGGACGA AGGCCATATC 
GAGGATGCTG AGAAGGCACA GCAGCGCGAC CGCGAGAAGC ACGACGAGAG CGAGGAGCTA 
AGCAATAAGG ACATCCAGGA AAGCGACGAG GACGTGGCCG TCTCACTGCT GCGCGCCAAG 
AACCTATCGC TGCTGCAAAG CCAGGAAATA TCATCTAGCC AGCAGAACTT CTTCCGCATG 
CTGGACAAGA AGATTGATGA GGGTCCCGAC TACGATTCGG CCAGCGAGAC GGAGATCGCA 
TTGGAGGAGG CCCGACTAAA CGCCCTGCGT CAACATTGGG AGTCCGCCAG CATTACGGCC 
TCCATCTGCT CCTCGGCCAG CCGCTCGCTG CAGACGACGC CCATCCGCCA GGTGCAGGTG 
CCATTGAAGC AGCCACCGCG TGGCGCCGGC CTGCTGATTC AGCCTTCTAG CGCCTCGGCG 
GTCACCGCCT CCACCTCCTC CTCCTCCTCA TCGACGATAG TCGCGCTGCC CACGACGGAG 
TATCTACCTC AGCATGTGTT GACGGGACGG CAAGTGGTGC AGCAGCAGCA ACAACAGCAG 
CAGCAACAGG CGGCAGTGCT CTACCAGCAG CAGCAGCAGC AACTAATACT GCTGCCCCAG 
TTGGATCCTA ATGTTGTCAA CACGTCCACG GCAGCAGCAG CGGCACAGCT CGTCCAGTTG 
CAGCAGCAGC ATCAACAGCA GCAACAACAG CTACAGCTGC AGCAGCAACA GCAGCTGCAA 
CTCAATCAGC AGCAGCAGCA GCAACTTTAC CAGCAGCAGC AACAGCAATA CCTGCTGACC 
CTTCCCGCCG GATCAAAGCC CCAGAGCGTG AGTCCAAAGC GCCTGATCTA TGGTGGACCC 
GCTGCAACCA CTCTCCTCTG TGCAACTGGC ATCAAATGCA ACGCCCCCGA AACTTACGAA 
CCGCCAACCG CTCCACACAG CCAAATCCAG CAGCAACAGC CACCGCCTCC TCCGCCGGGC 
GCCTACTACG GCGAGATGAT GCACGGCGTA CAGGCCGCTC CAGCGGCGGA ATACAAGTTC 
CCGCCGGCCA TCAGCGTGCA GAGACTGGCG CCGCAGGTGC AGCGGCAGTT GCGCGAGACG 
CAGGAACTCA TAAAAGACTC GTGCCCGCAG CTGTATGCGG CGGGCTATGG CAGCCCAGGA 
CCACCGATAC GCAATCCCAG CAGGAACCCC ACTAGAACTA GACCCACCCT GGAGACGCAG 
TTCTCGCAGG AACTCTCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tow ( NM_001274561.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261490.1 Drosophila melanogaster target of wingless (tow), transcript variant G, mRNA.

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CloneID	OFa20632
Clone ID Related Accession (Same CDS sequence)	NM_001274561.1
Accession Version	NM_001274561.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	target of wingless, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCTGTG GACAGAGCAA GATACATTTG TATCCCAGAA AATCGAAGAG TAAAGCGAAC 
GGCAAGAAAG GAGGACATGC CGACTCAGAT GCCGAAACGG ACGAGGACGA AGGCCATATC 
GAGGATGCTG AGAAGGCACA GCAGCGCGAC CGCGAGAAGC ACGACGAGAG CGAGGAGCTA 
AGCAATAAGG ACATCCAGGA AAGCGACGAG GACGTGGCCG TCTCACTGCT GCGCGCCAAG 
AACCTATCGC TGCTGCAAAG CCACCAGCAG AACTTCTTCC GCATGCTGGA CAAGAAGATT 
GATGAGGGTC CCGACTACGA TTCGGCCAGC GAGACGGAGA TCGCATTGGA GGAGGCCCGA 
CTAAACGCCC TGCGTCAACA TTGGGAGTCC GCCAGCATTA CGGCCTCCAT CTGCTCCTCG 
GCCAGCCGCT CGCTGCAGAC GACGCCCATC CGCCAGGTGC AGGTGCCATT GAAGCAGCCA 
CCGCGTGGCG CCGGCCTGCT GATTCAGCCT TCTAGCGCCT CGGCGGTCAC CGCCTCCACC 
TCCTCCTCCT CCTCATCGAC GATAGTCGCG CTGCCCACGA CGGAGTATCT ACCTCAGCAT 
GTGTTGACGG GACGGCAAGT GGTGCAGCAG CAGCAACAAC AGCAGCAGCA ACAGGCGGCA 
GTGCTCTACC AGCAGCAGCA GCAGCAACTA ATACTGCTGC CCCAGTTGGA TCCTAATGTT 
GTCAACACGT CCACGGCAGC AGCAGCGGCA CAGCTCGTCC AGTTGCAGCA GCAGCATCAA 
CAGCAGCAAC AACAGCTACA GCTGCAGCAG CAACAGCAGC TGCAACTCAA TCAGCAGCAG 
CAGCAGCAAC TTTACCAGCA GCAGCAACAG CAATACCTGC TGACCCTTCC CGCCGGATCA 
AAGCCCCAGA GCGTGAGTCC AAAGCGCCTG ATCTATGGTG GACCCGCTGC AACCACTCTC 
CTCTGTGCAA CTGGTGATGT GCATTTCAGC ATCAAATGCA ACGCCCCCGA AACTTACGAA 
CCGCCAACCG CTCCACACAG CCAAATCCAG CAGCAACAGC CACCGCCTCC TCCGCCGGGC 
GCCTACTACG GCGAGATGAT GCACGGCGTA CAGGCCGCTC CAGCGGCGGA ATACAAGTTC 
CCGCCGGCCA TCAGCGTGCA GAGACTGGCG CCGCAGGTGC AGCGGCAGTT GCGCGAGACG 
CAGGAACTCA TAAAAGACTC GTGCCCGCAG CTGTATGCGG CGGGCTATGG CAGCCCAGGA 
CCACCGATAC GCAATCCCAG CAGGAACCCC ACTAGAACTA GACCCACCCT GGAGACGCAG 
TTCTCGCAGG AACTCTCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261490.1
CDS	506..1846
Translation	MGCGQSKIHLYPRKSKSKANGKKGGHADSDAETDEDEGHIEDAEKAQQRDREKHDESEELSNKDIQESDEDVAVSLLRAKNLSLLQSHQQNFFRMLDKKIDEGPDYDSASETEIALEEARLNALRQHWESASITASICSSASRSLQTTPIRQVQVPLKQPPRGAGLLIQPSSASAVTASTSSSSSSTIVALPTTEYLPQHVLTGRQVVQQQQQQQQQQAAVLYQQQQQQLILLPQLDPNVVNTSTAAAAAQLVQLQQQHQQQQQQLQLQQQQQLQLNQQQQQQLYQQQQQQYLLTLPAGSKPQSVSPKRLIYGGPAATTLLCATGDVHFSIKCNAPETYEPPTAPHSQIQQQQPPPPPPGAYYGEMMHGVQAAPAAEYKFPPAISVQRLAPQVQRQLRETQELIKDSCPQLYAAGYGSPGPPIRNPSRNPTRTRPTLETQFSQELS

Target ORF information:

RefSeq Version	NM_001274561.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster target of wingless (tow), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274561.1

ORF Insert Sequence:

ATGGGCTGTG GACAGAGCAA GATACATTTG TATCCCAGAA AATCGAAGAG TAAAGCGAAC 
GGCAAGAAAG GAGGACATGC CGACTCAGAT GCCGAAACGG ACGAGGACGA AGGCCATATC 
GAGGATGCTG AGAAGGCACA GCAGCGCGAC CGCGAGAAGC ACGACGAGAG CGAGGAGCTA 
AGCAATAAGG ACATCCAGGA AAGCGACGAG GACGTGGCCG TCTCACTGCT GCGCGCCAAG 
AACCTATCGC TGCTGCAAAG CCACCAGCAG AACTTCTTCC GCATGCTGGA CAAGAAGATT 
GATGAGGGTC CCGACTACGA TTCGGCCAGC GAGACGGAGA TCGCATTGGA GGAGGCCCGA 
CTAAACGCCC TGCGTCAACA TTGGGAGTCC GCCAGCATTA CGGCCTCCAT CTGCTCCTCG 
GCCAGCCGCT CGCTGCAGAC GACGCCCATC CGCCAGGTGC AGGTGCCATT GAAGCAGCCA 
CCGCGTGGCG CCGGCCTGCT GATTCAGCCT TCTAGCGCCT CGGCGGTCAC CGCCTCCACC 
TCCTCCTCCT CCTCATCGAC GATAGTCGCG CTGCCCACGA CGGAGTATCT ACCTCAGCAT 
GTGTTGACGG GACGGCAAGT GGTGCAGCAG CAGCAACAAC AGCAGCAGCA ACAGGCGGCA 
GTGCTCTACC AGCAGCAGCA GCAGCAACTA ATACTGCTGC CCCAGTTGGA TCCTAATGTT 
GTCAACACGT CCACGGCAGC AGCAGCGGCA CAGCTCGTCC AGTTGCAGCA GCAGCATCAA 
CAGCAGCAAC AACAGCTACA GCTGCAGCAG CAACAGCAGC TGCAACTCAA TCAGCAGCAG 
CAGCAGCAAC TTTACCAGCA GCAGCAACAG CAATACCTGC TGACCCTTCC CGCCGGATCA 
AAGCCCCAGA GCGTGAGTCC AAAGCGCCTG ATCTATGGTG GACCCGCTGC AACCACTCTC 
CTCTGTGCAA CTGGTGATGT GCATTTCAGC ATCAAATGCA ACGCCCCCGA AACTTACGAA 
CCGCCAACCG CTCCACACAG CCAAATCCAG CAGCAACAGC CACCGCCTCC TCCGCCGGGC 
GCCTACTACG GCGAGATGAT GCACGGCGTA CAGGCCGCTC CAGCGGCGGA ATACAAGTTC 
CCGCCGGCCA TCAGCGTGCA GAGACTGGCG CCGCAGGTGC AGCGGCAGTT GCGCGAGACG 
CAGGAACTCA TAAAAGACTC GTGCCCGCAG CTGTATGCGG CGGGCTATGG CAGCCCAGGA 
CCACCGATAC GCAATCCCAG CAGGAACCCC ACTAGAACTA GACCCACCCT GGAGACGCAG 
TTCTCGCAGG AACTCTCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tow ( NM_001274560.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261489.1 Drosophila melanogaster target of wingless (tow), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa20633
Clone ID Related Accession (Same CDS sequence)	NM_001274560.1
Accession Version	NM_001274560.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	target of wingless, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCTGTG GACAGAGCAA GATACATTTG TATCCCAGAA AATCGAAGAG TAAAGCGAAC 
GGCAAGAAAG GAGGACATGC CGACTCAGAT GCCGAAACGG ACGAGGACGA AGGCCATATC 
GAGGATGCTG AGAAGGCACA GCAGCGCGAC CGCGAGAAGC ACGACGAGAG CGAGGAGCTA 
AGCAATAAGG ACATCCAGGA AAGCGACGAG GACGTGGCCG TCTCACTGCT GCGCGCCAAG 
AACCTATCGC TGCTGCAAAG CCAGGAAATA TCATCTAGCC AGCAGAACTT CTTCCGCATG 
CTGGACAAGA AGATTGATGA GGGTCCCGAC TACGATTCGG CCAGCGAGAC GGAGATCGCA 
TTGGAGGAGG CCCGACTAAA CGCCCTGCGT CAACATTGGG AGTCCGCCAG CATTACGGCC 
TCCATCTGCT CCTCGGCCAG CCGCTCGCTG CAGACGACGC CCATCCGCCA GGTGCAGGTG 
CCATTGAAGC AGCCACCGCG TGGCGCCGGC CTGCTGATTC AGCCTTCTAG CGCCTCGGCG 
GTCACCGCCT CCACCTCCTC CTCCTCCTCA TCGACGATAG TCGCGCTGCC CACGACGGAG 
TATCTACCTC AGCATGTGTT GACGGGACGG CAAGTGGTGC AGCAGCAGCA ACAACAGCAG 
CAGCAACAGG CGGCAGTGCT CTACCAGCAG CAGCAGCAGC AACTAATACT GCTGCCCCAG 
TTGGATCCTA ATGTTGTCAA CACGTCCACG GCAGCAGCAG CGGCACAGCT CGTCCAGTTG 
CAGCAGCAGC ATCAACAGCA GCAACAACAG CTACAGCTGC AGCAGCAACA GCAGCTGCAA 
CTCAATCAGC AGCAGCAGCA GCAACTTTAC CAGCAGCAGC AACAGCAATA CCTGCTGACC 
CTTCCCGCCG GATCAAAGCC CCAGAGCGTG AGTCCAAAGC GCCTGATCTA TGGTGGACCC 
GCTGCAACCA CTCTCCTCTG TGCAACTGGT GATGTGCATT TCAGCATCAA ATGCAACGCC 
CCCGAAACTT ACGAACCGCC AACCGCTCCA CACAGCCAAA TCCAGCAGCA ACAGCCACCG 
CCTCCTCCGC CGGGCGCCTA CTACGGCGAG ATGATGCACG GCGTACAGGC CGCTCCAGCG 
GCGGAATACA AGTTCCCGCC GGCCATCAGC GTGCAGAGAC TGGCGCCGCA GGTGCAGCGG 
CAGTTGCGCG AGACGCAGGA ACTCATAAAA GACTCGTGCC CGCAGCTGTA TGCGGCGGGC 
TATGGCAGCC CAGGACCACC GATACGCAAT CCCAGCAGGA ACCCCACTAG AACTAGACCC 
ACCCTGGAGA CGCAGTTCTC GCAGGAACTC TCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261489.1
CDS	411..1766
Translation	MGCGQSKIHLYPRKSKSKANGKKGGHADSDAETDEDEGHIEDAEKAQQRDREKHDESEELSNKDIQESDEDVAVSLLRAKNLSLLQSQEISSSQQNFFRMLDKKIDEGPDYDSASETEIALEEARLNALRQHWESASITASICSSASRSLQTTPIRQVQVPLKQPPRGAGLLIQPSSASAVTASTSSSSSSTIVALPTTEYLPQHVLTGRQVVQQQQQQQQQQAAVLYQQQQQQLILLPQLDPNVVNTSTAAAAAQLVQLQQQHQQQQQQLQLQQQQQLQLNQQQQQQLYQQQQQQYLLTLPAGSKPQSVSPKRLIYGGPAATTLLCATGDVHFSIKCNAPETYEPPTAPHSQIQQQQPPPPPPGAYYGEMMHGVQAAPAAEYKFPPAISVQRLAPQVQRQLRETQELIKDSCPQLYAAGYGSPGPPIRNPSRNPTRTRPTLETQFSQELS

Target ORF information:

RefSeq Version	NM_001274560.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster target of wingless (tow), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274560.1

ORF Insert Sequence:

ATGGGCTGTG GACAGAGCAA GATACATTTG TATCCCAGAA AATCGAAGAG TAAAGCGAAC 
GGCAAGAAAG GAGGACATGC CGACTCAGAT GCCGAAACGG ACGAGGACGA AGGCCATATC 
GAGGATGCTG AGAAGGCACA GCAGCGCGAC CGCGAGAAGC ACGACGAGAG CGAGGAGCTA 
AGCAATAAGG ACATCCAGGA AAGCGACGAG GACGTGGCCG TCTCACTGCT GCGCGCCAAG 
AACCTATCGC TGCTGCAAAG CCAGGAAATA TCATCTAGCC AGCAGAACTT CTTCCGCATG 
CTGGACAAGA AGATTGATGA GGGTCCCGAC TACGATTCGG CCAGCGAGAC GGAGATCGCA 
TTGGAGGAGG CCCGACTAAA CGCCCTGCGT CAACATTGGG AGTCCGCCAG CATTACGGCC 
TCCATCTGCT CCTCGGCCAG CCGCTCGCTG CAGACGACGC CCATCCGCCA GGTGCAGGTG 
CCATTGAAGC AGCCACCGCG TGGCGCCGGC CTGCTGATTC AGCCTTCTAG CGCCTCGGCG 
GTCACCGCCT CCACCTCCTC CTCCTCCTCA TCGACGATAG TCGCGCTGCC CACGACGGAG 
TATCTACCTC AGCATGTGTT GACGGGACGG CAAGTGGTGC AGCAGCAGCA ACAACAGCAG 
CAGCAACAGG CGGCAGTGCT CTACCAGCAG CAGCAGCAGC AACTAATACT GCTGCCCCAG 
TTGGATCCTA ATGTTGTCAA CACGTCCACG GCAGCAGCAG CGGCACAGCT CGTCCAGTTG 
CAGCAGCAGC ATCAACAGCA GCAACAACAG CTACAGCTGC AGCAGCAACA GCAGCTGCAA 
CTCAATCAGC AGCAGCAGCA GCAACTTTAC CAGCAGCAGC AACAGCAATA CCTGCTGACC 
CTTCCCGCCG GATCAAAGCC CCAGAGCGTG AGTCCAAAGC GCCTGATCTA TGGTGGACCC 
GCTGCAACCA CTCTCCTCTG TGCAACTGGT GATGTGCATT TCAGCATCAA ATGCAACGCC 
CCCGAAACTT ACGAACCGCC AACCGCTCCA CACAGCCAAA TCCAGCAGCA ACAGCCACCG 
CCTCCTCCGC CGGGCGCCTA CTACGGCGAG ATGATGCACG GCGTACAGGC CGCTCCAGCG 
GCGGAATACA AGTTCCCGCC GGCCATCAGC GTGCAGAGAC TGGCGCCGCA GGTGCAGCGG 
CAGTTGCGCG AGACGCAGGA ACTCATAAAA GACTCGTGCC CGCAGCTGTA TGCGGCGGGC 
TATGGCAGCC CAGGACCACC GATACGCAAT CCCAGCAGGA ACCCCACTAG AACTAGACCC 
ACCCTGGAGA CGCAGTTCTC GCAGGAACTC TCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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tow cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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