Gen cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gen
Entrez Gene ID	38594
Full Name	XPG-like endonuclease
Synonyms	BcDNA:GM10765,CG10670,DmGEN,Dmel\CG10670,mus324
General protein information	Preferred Names XPG-like endonuclease Names CG10670 gene product from transcript CG10670-RA CG10670-PA Gen-PA mutagen-sensitive 324
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 64C7-64C7

mRNA and Protein(s)

mRNA	Protein	Name
NM_139686.3	NP_647943.2	XPG-like endonuclease

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Gen	NP_647943.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002669	XP_312255.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the in vitro activities of DmGen and HsGEN1 are strikingly similar.
Title: Substrate preference of Gen endonucleases highlights the importance of branched structures as DNA damage repair intermediates.

DmGEN belongs to a new class (class IV) of the RAD2/XPG nuclease family
Title: DmGEN shows a flap endonuclease activity, cleaving the blocked-flap structure and model replication fork.

Cloning and characterization of DmGEN, Drosophila Melanogaster XPG-like endonuclease, a RAD2 nuclease family member.
Title: DmGEN, a novel RAD2 family endo-exonuclease from Drosophila melanogaster.

The following Gen gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gen cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17566	NM_139686.3 Latest version!	Drosophila melanogaster XPG-like endonuclease (Gen), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gen ( NM_139686.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_647943.2 Drosophila melanogaster XPG-like endonuclease (Gen), mRNA.

Price & Availability↑

CloneID	OFa17566
Clone ID Related Accession (Same CDS sequence)	NM_139686.3
Accession Version	NM_139686.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	XPG-like endonuclease
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_139686.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGTCA AGGAATTATG GGGTGTTTTA ACGCCCCATT GTGAACGTAA ACCCATTAAT 
GAACTGCGGG GAAAGAAAGT GGCCATCGAC CTGGCCGGCT GGGTGTGCGA ATCTCTTAAT 
GTTGTGGACT ACTTTGTGCA TCCAAGGCAT CATTTAAAGA ACCTCTTCTT TCGCACTTGC 
TACTTGATTT GGGAGCAAGT TACTCCGGTT TTCGTTTTGG AGGGAGTTGC TCCAAAACTC 
AAGAGCCAGG TGATAGCCAA AAGGAATGAG CTCCAGTTTC GGGGAGTGAA GCCCAAGAAT 
TCGCCTGAGT GCACCCAAAG TCAGCCGTCC AAGGGCGACA AGGGACGCAG CCGGTTCAAC 
CATGTTCTCA AGCAGTGCGA AACGCTGCTG CTCTCCATGG GAATCCAGTG CGTCCAAGGT 
CCCGGCGAGG CGGAGGCCTA CTGTGCCTTT CTCAACAAGC ATGGCCTAGT GGACGGCGTC 
ATTAGCCAGG ACTCGGATTG CTTTGCCTAT GGAGCCGTTC GTGTCTATCG CAACTTCTCC 
GTTTCCACCC AAGGAGCCCA GGCAGCCGCC GGCGGAGCTG TGGACATCTA CGACATGCGG 
GAGATCACAT CCCGAATGGA CTTTGGCCAA CAGAAGATCA TTGTGATGGC TTTGCTCTGC 
GGCTGTGATT ATTGTCCTGA TGGCATAGGT GGCATTGGCA AGGATGGCGT CCTAAAGCTG 
TTCAACAAGT ACAAGGAGAC TGAGATTTTG GATAGAATGA GAAGCTGGCG TGGTGAAACC 
GATAAATACA ATGCCTTGGA GATCCGAGTG GACGACAAAT CCATCTGCAG CAATTGTGGC 
CACATTGGCA AGACCCAGAG CCACACAAAA AGTGGCTGCA GTGTGTGTCG CACACACAAA 
GGCTGCGATG AGAGTCTCTG GAAAGAACAA CGACTATCCA TTAAGTCCGA GTTGACTTTG 
AGGCGGAAAG CCCTATTATC TCCTGACTTT CCCAACGAAG AGATCATTGC CGAGTTTCTC 
AGCGAACCCG ACACCATTCC CAATCTAAAT CTCAACTGGC GGCAACCAAA TCTTGTCAAA 
TTCATCAAAC AAATCGGACA TCTCTTGCAG TGGCCAGAAA TATACTGCTT CCAAAAGTTC 
TTTCCCATAC TCACACGCTG GCAGGTGCAG CAATCGAAGC AGGAGAAGAT ACTTATCCAG 
CCACACGAGA TTATCAAAAA GCGAACTGTG AAGGGTGTGC CCAGCTTAGA ATTGCGCTGG 
CACGATCCCA GTGGCATCTT CAAGGGTCTT ATTCCGGACA AGCAGATTGC GGAGTACGAA 
GCGGAGCATC CCAAGGGAAT CGAGGAACTT TACTACACCA TTGAGCCATT GGACATGCTG 
GAGACAGCGT ATCCCGATTT AGTAGCGGCC TTTTTGAAGT CGAAGGAGAA GCCCGCCAAG 
AAAACCACGC GAAAGAAGAA AACCGCATCT GAAGAGGAGA ATAAGGAAAA CGAGCCGAAT 
TCGAAACCAA AGCGAGTGGT CAGAAAGATA AAGGCTCAGC CTGAAGAGAA CCAGCCATTA 
CTGCACCAAT TCCTAGGGCG CAAGAAGGAA GGTACTCCGG TCAAGGCTCC CGCTCCGCAA 
CGTCAGCAAT GCTCCACCCC CATCACCAAG TTCCTGCCCT CCGATCTGGA AAGCGATTGC 
GATGCCGAAG AGTTCGATAT GTCGGACATA GTTAAAGGCA TTATCTCGAA TCCGAATGCC 
AAGCCAGCAC TGACCAACCA CGATGGTCAC CAGCTTCATT ACGAACCCAT GGCGGAAGAT 
TTGAGCTTAC GTCTGGCGCA GATGAGTCTA GGAAATGTGA ATGAATCGCC CAAAGTGGAA 
ACGAAAAGAG ATCTCTCTCA AGTGGATCAA CTACCGCAGA GTAAGCGATT CTCCTTGGAG 
GACAGTTTTG ATCTGCTGGT CAAGGGAGAT CTGCAGAAGC TGGCCAGGAC GCCAGTGGAG 
CGATTCAAGA TGCAGCATCG CATCAGCGAA AAGATTCCAA CGCCAGTGAA GCCCTTGGAT 
AACATAAGTT ACTTCTTCAA TCAAAGTTCC GATAATGCGG ATGTCTTTGA AGAGTTGATG 
AACTCCAGCT TGGTGCCCCA GGATCAAGAG GATAACGCAG AAGATGAGGA GGAGGATGAC 
CTGGTAGTGA TTAGTGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_647943.2
CDS	76..2256
Translation	MGVKELWGVLTPHCERKPINELRGKKVAIDLAGWVCESLNVVDYFVHPRHHLKNLFFRTCYLIWEQVTPVFVLEGVAPKLKSQVIAKRNELQFRGVKPKNSPECTQSQPSKGDKGRSRFNHVLKQCETLLLSMGIQCVQGPGEAEAYCAFLNKHGLVDGVISQDSDCFAYGAVRVYRNFSVSTQGAQAAAGGAVDIYDMREITSRMDFGQQKIIVMALLCGCDYCPDGIGGIGKDGVLKLFNKYKETEILDRMRSWRGETDKYNALEIRVDDKSICSNCGHIGKTQSHTKSGCSVCRTHKGCDESLWKEQRLSIKSELTLRRKALLSPDFPNEEIIAEFLSEPDTIPNLNLNWRQPNLVKFIKQIGHLLQWPEIYCFQKFFPILTRWQVQQSKQEKILIQPHEIIKKRTVKGVPSLELRWHDPSGIFKGLIPDKQIAEYEAEHPKGIEELYYTIEPLDMLETAYPDLVAAFLKSKEKPAKKTTRKKKTASEEENKENEPNSKPKRVVRKIKAQPEENQPLLHQFLGRKKEGTPVKAPAPQRQQCSTPITKFLPSDLESDCDAEEFDMSDIVKGIISNPNAKPALTNHDGHQLHYEPMAEDLSLRLAQMSLGNVNESPKVETKRDLSQVDQLPQSKRFSLEDSFDLLVKGDLQKLARTPVERFKMQHRISEKIPTPVKPLDNISYFFNQSSDNADVFEELMNSSLVPQDQEDNAEDEEEDDLVVISD

Target ORF information:

RefSeq Version	NM_139686.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster XPG-like endonuclease (Gen), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139686.3

ORF Insert Sequence:

ATGGGCGTCA AGGAATTATG GGGTGTTTTA ACGCCCCATT GTGAACGTAA ACCCATTAAT 
GAACTGCGGG GAAAGAAAGT GGCCATCGAC CTGGCCGGCT GGGTGTGCGA ATCTCTTAAT 
GTTGTGGACT ACTTTGTGCA TCCAAGGCAT CATTTAAAGA ACCTCTTCTT TCGCACTTGC 
TACTTGATTT GGGAGCAAGT TACTCCGGTT TTCGTTTTGG AGGGAGTTGC TCCAAAACTC 
AAGAGCCAGG TGATAGCCAA AAGGAATGAG CTCCAGTTTC GGGGAGTGAA GCCCAAGAAT 
TCGCCTGAGT GCACCCAAAG TCAGCCGTCC AAGGGCGACA AGGGACGCAG CCGGTTCAAC 
CATGTTCTCA AGCAGTGCGA AACGCTGCTG CTCTCCATGG GAATCCAGTG CGTCCAAGGT 
CCCGGCGAGG CGGAGGCCTA CTGTGCCTTT CTCAACAAGC ATGGCCTAGT GGACGGCGTC 
ATTAGCCAGG ACTCGGATTG CTTTGCCTAT GGAGCCGTTC GTGTCTATCG CAACTTCTCC 
GTTTCCACCC AAGGAGCCCA GGCAGCCGCC GGCGGAGCTG TGGACATCTA CGACATGCGG 
GAGATCACAT CCCGAATGGA CTTTGGCCAA CAGAAGATCA TTGTGATGGC TTTGCTCTGC 
GGCTGTGATT ATTGTCCTGA TGGCATAGGT GGCATTGGCA AGGATGGCGT CCTAAAGCTG 
TTCAACAAGT ACAAGGAGAC TGAGATTTTG GATAGAATGA GAAGCTGGCG TGGTGAAACC 
GATAAATACA ATGCCTTGGA GATCCGAGTG GACGACAAAT CCATCTGCAG CAATTGTGGC 
CACATTGGCA AGACCCAGAG CCACACAAAA AGTGGCTGCA GTGTGTGTCG CACACACAAA 
GGCTGCGATG AGAGTCTCTG GAAAGAACAA CGACTATCCA TTAAGTCCGA GTTGACTTTG 
AGGCGGAAAG CCCTATTATC TCCTGACTTT CCCAACGAAG AGATCATTGC CGAGTTTCTC 
AGCGAACCCG ACACCATTCC CAATCTAAAT CTCAACTGGC GGCAACCAAA TCTTGTCAAA 
TTCATCAAAC AAATCGGACA TCTCTTGCAG TGGCCAGAAA TATACTGCTT CCAAAAGTTC 
TTTCCCATAC TCACACGCTG GCAGGTGCAG CAATCGAAGC AGGAGAAGAT ACTTATCCAG 
CCACACGAGA TTATCAAAAA GCGAACTGTG AAGGGTGTGC CCAGCTTAGA ATTGCGCTGG 
CACGATCCCA GTGGCATCTT CAAGGGTCTT ATTCCGGACA AGCAGATTGC GGAGTACGAA 
GCGGAGCATC CCAAGGGAAT CGAGGAACTT TACTACACCA TTGAGCCATT GGACATGCTG 
GAGACAGCGT ATCCCGATTT AGTAGCGGCC TTTTTGAAGT CGAAGGAGAA GCCCGCCAAG 
AAAACCACGC GAAAGAAGAA AACCGCATCT GAAGAGGAGA ATAAGGAAAA CGAGCCGAAT 
TCGAAACCAA AGCGAGTGGT CAGAAAGATA AAGGCTCAGC CTGAAGAGAA CCAGCCATTA 
CTGCACCAAT TCCTAGGGCG CAAGAAGGAA GGTACTCCGG TCAAGGCTCC CGCTCCGCAA 
CGTCAGCAAT GCTCCACCCC CATCACCAAG TTCCTGCCCT CCGATCTGGA AAGCGATTGC 
GATGCCGAAG AGTTCGATAT GTCGGACATA GTTAAAGGCA TTATCTCGAA TCCGAATGCC 
AAGCCAGCAC TGACCAACCA CGATGGTCAC CAGCTTCATT ACGAACCCAT GGCGGAAGAT 
TTGAGCTTAC GTCTGGCGCA GATGAGTCTA GGAAATGTGA ATGAATCGCC CAAAGTGGAA 
ACGAAAAGAG ATCTCTCTCA AGTGGATCAA CTACCGCAGA GTAAGCGATT CTCCTTGGAG 
GACAGTTTTG ATCTGCTGGT CAAGGGAGAT CTGCAGAAGC TGGCCAGGAC GCCAGTGGAG 
CGATTCAAGA TGCAGCATCG CATCAGCGAA AAGATTCCAA CGCCAGTGAA GCCCTTGGAT 
AACATAAGTT ACTTCTTCAA TCAAAGTTCC GATAATGCGG ATGTCTTTGA AGAGTTGATG 
AACTCCAGCT TGGTGCCCCA GGATCAAGAG GATAACGCAG AAGATGAGGA GGAGGATGAC 
CTGGTAGTGA TTAGTGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Gen cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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