GC cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	GC
Entrez Gene ID	38194
Full Name	gamma-glutamyl carboxylase
Synonyms	CG13927,DgammaC,Dmel\CG13927,dGC,dgammaC,gammaC
General protein information	Preferred Names gamma-glutamyl carboxylase Names CG13927 gene product from transcript CG13927-RA CG13927-PA CG13927-PB GC-PA GC-PB gamma-glutamyl-carboxylase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 62A9-62A9

mRNA and Protein(s)

mRNA	Protein	Name
NM_079161.4	NP_523885.2	gamma-glutamyl carboxylase, isoform A
NM_001169852.1	NP_001163323.1	gamma-glutamyl carboxylase, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GGCX	XP_515586.2
Canis lupus familiaris (dog)	GGCX	XP_532979.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006363	XP_316389.4
Homo sapiens (human)	GGCX	NP_000812.2
	GGCX	XP_001086474.1
Bos taurus (cattle)	GGCX	NP_776491.1
Mus musculus (house mouse)	Ggcx	NP_062776.1
Rattus norvegicus (Norway rat)	Ggcx	NP_113944.1
Xenopus tropicalis (tropical clawed frog)	ggcx	NP_001116905.1
Danio rerio (zebrafish)	LOC100537604	XP_003199342.1
Drosophila melanogaster (fruit fly)	GC	NP_001163323.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa17186	NM_079161.4 Latest version!	Drosophila melanogaster gamma-glutamyl carboxylase (GC), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa17186	NM_001169852.1 Latest version!	Drosophila melanogaster gamma-glutamyl carboxylase (GC), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GC ( NM_079161.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523885.2 Drosophila melanogaster gamma-glutamyl carboxylase (GC), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa17186
Clone ID Related Accession (Same CDS sequence)	NM_079161.4 , NM_001169852.1
Accession Version	NM_079161.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	gamma-glutamyl carboxylase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079161.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAACT CCAAGCGAAA GCTGCCCACG AAACCCAGTG CCACAGAGGA CGCTACTCCG 
CACAAAGATA CACCTAGGTC GTCGGAAGAA TTCCCCACAG ATGTAAATCA CAAGAGTGCC 
AAGAAGTCCT TCCTTCCTCA AAACGCATTC TTTCGAGATT GCCTCGGTCA CGATCTGAGC 
AACTTCACTA GCCTGACCAA CTTTACCAGC TGGCTAAACA GACCCGTAGA TGGAGCTGCC 
CTGGGCATCT TCCGTCTGCT CTACGGAGCA GCCATGCTGA TTGACATCGC AGAGGAGCGA 
GGTGGCGGCC AGCTGGACGT GCGGTTTCAG GAGCCGCTAC ACTGCCACTT TCCACTGTTC 
AACGGGATGC GAGCATTGGA CTATCCACTG ATGGGCTGCG TCTATCTGTG CATGTGGCTG 
GGCGCGTGGG GCATCATGCT GGGCTATCGA TTCAGGACTA GCTGCCTGGC CTTCCTGATA 
CCCTACTGGT ACATCTTCCT GCTGGACAAA CCGACGTGGA ATAACCACAG CTATCTATTC 
GGCCTGGTGG GAACATTGCT GCTCTTCACC CAAGCGGAGT CCTACTGCTC CCTGGACAGA 
TGGCTAAATC CCCGAAAGAG TCACTGTATT CCCTACTGGA ACTACTTCCT AATCAAATTC 
CAGTTCTTCA TCCTGTACAT GTACGCAGGT CTGAAGAAAT TCTCGCTGGA ATGGCTATCG 
GGATACGCGA TGTCCAATCT GAGTTACCAC TGGGTCTTTG CCCCGTTCCG GCAAGTGATT 
GATCCGGAGC TGATAGACCT GCTCATCGTG CATTGGTTCA CCGCCTTCTT CGACGTGTCC 
ATTGCTTTTT TCATGACTCT GGAAAAGACG CGCCTACTGG TCACTCCTTT CATGCTCAGT 
TTTCATCTAA TGAACTCCCG ACTGTTTGTA ATAGGCATGT TTCCCTGGGT GTGTCTGGCC 
GAAGTGCCGC TCTTCTTCAG CTTCGACTGG CCGAGGAGGC TAGGCAACTG GTCCAAAATA 
CAGGACGTCC ATCCAAAAGA TCCAGAGGAA GAGCTTTCCA AGCCGGGAAT CCTGGCCAGA 
CTGCGCACGG GCCTCATCCT TGCCTACTGT GGACTGCAAC TCTTCCTGCC ATACTCACAC 
TTCATCACCA AGGGCTATAA CAACTGGACG AATGGGCTGT ACGGCTACTC ATGGGACATG 
ATGGTGCACT CCTACGACAC GCTGCAGACC TCCATTCAGG TGGTGGACAA CGAGAGCCGC 
CAAGTGCACA ACCTGAATCC ATACGCCTTT ACGGAGTACG ACCGCTGGAC GAAGTACGCC 
GACATGGCAG TGCAGTATGC CAGGTGCATC GAGGAGAATT TACAAGAGAA ACAGCCACAT 
ATTGGGAGGA ACATTTCCAT CTACTTCGAC ATCTGGTGCT CCATGAACGG ACGCTTTCAG 
CAACGATCAT TCGATCCACG CGAGGATCTC CTCCGGGCCA AGTGGTCGCC CTTTGAGAGC 
ACCTCCTGGT CGCTGCCTCT GCTGAACGAG CTGAACCACA TGCGGCCCAA ATTGCGCACC 
ATTGAGACTG AGGTGCTGGC CTGGAACAAC TACTCCGATG TCATTTTCGT GGCGGATTTC 
CCGGGACTAA CTCTGACCAA CTTCATCTCG CCGGACCTTA TCAACTGCAC GCTGACCATT 
CTTGAGGGAA ATGTTCGGTA CCGGAACGAG CGGGATCCGG AGGCCTATTT CCTGACAGCC 
GGCAAGAGCA TCGGTCTGGA GAGCAACATC ACCCACTTGG TCACCACCAT TGGCCAGAAG 
CCAGCTTCGT ATCTGTTCAC ATACACCAAC AAAACGATGC TGGAGCAGGG CATCACGATA 
GAACAGCCTG ATACCGAGGA GCGGTCTGTC CTGCCGCTGT GGCAGGAGTT CCAGCATCGG 
GTTACCAACT ACCAGCAGTT TTTGGGCCAC ATTGGCAACT GCCTAATGTA CTTGCTCTAC 
GATGTGCCCA TTCCGCAGGC CGTAAAGGGG GGAGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523885.2
CDS	321..2339
Translation	MANSKRKLPTKPSATEDATPHKDTPRSSEEFPTDVNHKSAKKSFLPQNAFFRDCLGHDLSNFTSLTNFTSWLNRPVDGAALGIFRLLYGAAMLIDIAEERGGGQLDVRFQEPLHCHFPLFNGMRALDYPLMGCVYLCMWLGAWGIMLGYRFRTSCLAFLIPYWYIFLLDKPTWNNHSYLFGLVGTLLLFTQAESYCSLDRWLNPRKSHCIPYWNYFLIKFQFFILYMYAGLKKFSLEWLSGYAMSNLSYHWVFAPFRQVIDPELIDLLIVHWFTAFFDVSIAFFMTLEKTRLLVTPFMLSFHLMNSRLFVIGMFPWVCLAEVPLFFSFDWPRRLGNWSKIQDVHPKDPEEELSKPGILARLRTGLILAYCGLQLFLPYSHFITKGYNNWTNGLYGYSWDMMVHSYDTLQTSIQVVDNESRQVHNLNPYAFTEYDRWTKYADMAVQYARCIEENLQEKQPHIGRNISIYFDIWCSMNGRFQQRSFDPREDLLRAKWSPFESTSWSLPLLNELNHMRPKLRTIETEVLAWNNYSDVIFVADFPGLTLTNFISPDLINCTLTILEGNVRYRNERDPEAYFLTAGKSIGLESNITHLVTTIGQKPASYLFTYTNKTMLEQGITIEQPDTEERSVLPLWQEFQHRVTNYQQFLGHIGNCLMYLLYDVPIPQAVKGGD

Target ORF information:

RefSeq Version	NM_079161.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster gamma-glutamyl carboxylase (GC), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079161.4

ORF Insert Sequence:

ATGGCCAACT CCAAGCGAAA GCTGCCCACG AAACCCAGTG CCACAGAGGA CGCTACTCCG 
CACAAAGATA CACCTAGGTC GTCGGAAGAA TTCCCCACAG ATGTAAATCA CAAGAGTGCC 
AAGAAGTCCT TCCTTCCTCA AAACGCATTC TTTCGAGATT GCCTCGGTCA CGATCTGAGC 
AACTTCACTA GCCTGACCAA CTTTACCAGC TGGCTAAACA GACCCGTAGA TGGAGCTGCC 
CTGGGCATCT TCCGTCTGCT CTACGGAGCA GCCATGCTGA TTGACATCGC AGAGGAGCGA 
GGTGGCGGCC AGCTGGACGT GCGGTTTCAG GAGCCGCTAC ACTGCCACTT TCCACTGTTC 
AACGGGATGC GAGCATTGGA CTATCCACTG ATGGGCTGCG TCTATCTGTG CATGTGGCTG 
GGCGCGTGGG GCATCATGCT GGGCTATCGA TTCAGGACTA GCTGCCTGGC CTTCCTGATA 
CCCTACTGGT ACATCTTCCT GCTGGACAAA CCGACGTGGA ATAACCACAG CTATCTATTC 
GGCCTGGTGG GAACATTGCT GCTCTTCACC CAAGCGGAGT CCTACTGCTC CCTGGACAGA 
TGGCTAAATC CCCGAAAGAG TCACTGTATT CCCTACTGGA ACTACTTCCT AATCAAATTC 
CAGTTCTTCA TCCTGTACAT GTACGCAGGT CTGAAGAAAT TCTCGCTGGA ATGGCTATCG 
GGATACGCGA TGTCCAATCT GAGTTACCAC TGGGTCTTTG CCCCGTTCCG GCAAGTGATT 
GATCCGGAGC TGATAGACCT GCTCATCGTG CATTGGTTCA CCGCCTTCTT CGACGTGTCC 
ATTGCTTTTT TCATGACTCT GGAAAAGACG CGCCTACTGG TCACTCCTTT CATGCTCAGT 
TTTCATCTAA TGAACTCCCG ACTGTTTGTA ATAGGCATGT TTCCCTGGGT GTGTCTGGCC 
GAAGTGCCGC TCTTCTTCAG CTTCGACTGG CCGAGGAGGC TAGGCAACTG GTCCAAAATA 
CAGGACGTCC ATCCAAAAGA TCCAGAGGAA GAGCTTTCCA AGCCGGGAAT CCTGGCCAGA 
CTGCGCACGG GCCTCATCCT TGCCTACTGT GGACTGCAAC TCTTCCTGCC ATACTCACAC 
TTCATCACCA AGGGCTATAA CAACTGGACG AATGGGCTGT ACGGCTACTC ATGGGACATG 
ATGGTGCACT CCTACGACAC GCTGCAGACC TCCATTCAGG TGGTGGACAA CGAGAGCCGC 
CAAGTGCACA ACCTGAATCC ATACGCCTTT ACGGAGTACG ACCGCTGGAC GAAGTACGCC 
GACATGGCAG TGCAGTATGC CAGGTGCATC GAGGAGAATT TACAAGAGAA ACAGCCACAT 
ATTGGGAGGA ACATTTCCAT CTACTTCGAC ATCTGGTGCT CCATGAACGG ACGCTTTCAG 
CAACGATCAT TCGATCCACG CGAGGATCTC CTCCGGGCCA AGTGGTCGCC CTTTGAGAGC 
ACCTCCTGGT CGCTGCCTCT GCTGAACGAG CTGAACCACA TGCGGCCCAA ATTGCGCACC 
ATTGAGACTG AGGTGCTGGC CTGGAACAAC TACTCCGATG TCATTTTCGT GGCGGATTTC 
CCGGGACTAA CTCTGACCAA CTTCATCTCG CCGGACCTTA TCAACTGCAC GCTGACCATT 
CTTGAGGGAA ATGTTCGGTA CCGGAACGAG CGGGATCCGG AGGCCTATTT CCTGACAGCC 
GGCAAGAGCA TCGGTCTGGA GAGCAACATC ACCCACTTGG TCACCACCAT TGGCCAGAAG 
CCAGCTTCGT ATCTGTTCAC ATACACCAAC AAAACGATGC TGGAGCAGGG CATCACGATA 
GAACAGCCTG ATACCGAGGA GCGGTCTGTC CTGCCGCTGT GGCAGGAGTT CCAGCATCGG 
GTTACCAACT ACCAGCAGTT TTTGGGCCAC ATTGGCAACT GCCTAATGTA CTTGCTCTAC 
GATGTGCCCA TTCCGCAGGC CGTAAAGGGG GGAGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GC ( NM_001169852.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163323.1 Drosophila melanogaster gamma-glutamyl carboxylase (GC), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa17186
Clone ID Related Accession (Same CDS sequence)	NM_079161.4 , NM_001169852.1
Accession Version	NM_001169852.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	gamma-glutamyl carboxylase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAACT CCAAGCGAAA GCTGCCCACG AAACCCAGTG CCACAGAGGA CGCTACTCCG 
CACAAAGATA CACCTAGGTC GTCGGAAGAA TTCCCCACAG ATGTAAATCA CAAGAGTGCC 
AAGAAGTCCT TCCTTCCTCA AAACGCATTC TTTCGAGATT GCCTCGGTCA CGATCTGAGC 
AACTTCACTA GCCTGACCAA CTTTACCAGC TGGCTAAACA GACCCGTAGA TGGAGCTGCC 
CTGGGCATCT TCCGTCTGCT CTACGGAGCA GCCATGCTGA TTGACATCGC AGAGGAGCGA 
GGTGGCGGCC AGCTGGACGT GCGGTTTCAG GAGCCGCTAC ACTGCCACTT TCCACTGTTC 
AACGGGATGC GAGCATTGGA CTATCCACTG ATGGGCTGCG TCTATCTGTG CATGTGGCTG 
GGCGCGTGGG GCATCATGCT GGGCTATCGA TTCAGGACTA GCTGCCTGGC CTTCCTGATA 
CCCTACTGGT ACATCTTCCT GCTGGACAAA CCGACGTGGA ATAACCACAG CTATCTATTC 
GGCCTGGTGG GAACATTGCT GCTCTTCACC CAAGCGGAGT CCTACTGCTC CCTGGACAGA 
TGGCTAAATC CCCGAAAGAG TCACTGTATT CCCTACTGGA ACTACTTCCT AATCAAATTC 
CAGTTCTTCA TCCTGTACAT GTACGCAGGT CTGAAGAAAT TCTCGCTGGA ATGGCTATCG 
GGATACGCGA TGTCCAATCT GAGTTACCAC TGGGTCTTTG CCCCGTTCCG GCAAGTGATT 
GATCCGGAGC TGATAGACCT GCTCATCGTG CATTGGTTCA CCGCCTTCTT CGACGTGTCC 
ATTGCTTTTT TCATGACTCT GGAAAAGACG CGCCTACTGG TCACTCCTTT CATGCTCAGT 
TTTCATCTAA TGAACTCCCG ACTGTTTGTA ATAGGCATGT TTCCCTGGGT GTGTCTGGCC 
GAAGTGCCGC TCTTCTTCAG CTTCGACTGG CCGAGGAGGC TAGGCAACTG GTCCAAAATA 
CAGGACGTCC ATCCAAAAGA TCCAGAGGAA GAGCTTTCCA AGCCGGGAAT CCTGGCCAGA 
CTGCGCACGG GCCTCATCCT TGCCTACTGT GGACTGCAAC TCTTCCTGCC ATACTCACAC 
TTCATCACCA AGGGCTATAA CAACTGGACG AATGGGCTGT ACGGCTACTC ATGGGACATG 
ATGGTGCACT CCTACGACAC GCTGCAGACC TCCATTCAGG TGGTGGACAA CGAGAGCCGC 
CAAGTGCACA ACCTGAATCC ATACGCCTTT ACGGAGTACG ACCGCTGGAC GAAGTACGCC 
GACATGGCAG TGCAGTATGC CAGGTGCATC GAGGAGAATT TACAAGAGAA ACAGCCACAT 
ATTGGGAGGA ACATTTCCAT CTACTTCGAC ATCTGGTGCT CCATGAACGG ACGCTTTCAG 
CAACGATCAT TCGATCCACG CGAGGATCTC CTCCGGGCCA AGTGGTCGCC CTTTGAGAGC 
ACCTCCTGGT CGCTGCCTCT GCTGAACGAG CTGAACCACA TGCGGCCCAA ATTGCGCACC 
ATTGAGACTG AGGTGCTGGC CTGGAACAAC TACTCCGATG TCATTTTCGT GGCGGATTTC 
CCGGGACTAA CTCTGACCAA CTTCATCTCG CCGGACCTTA TCAACTGCAC GCTGACCATT 
CTTGAGGGAA ATGTTCGGTA CCGGAACGAG CGGGATCCGG AGGCCTATTT CCTGACAGCC 
GGCAAGAGCA TCGGTCTGGA GAGCAACATC ACCCACTTGG TCACCACCAT TGGCCAGAAG 
CCAGCTTCGT ATCTGTTCAC ATACACCAAC AAAACGATGC TGGAGCAGGG CATCACGATA 
GAACAGCCTG ATACCGAGGA GCGGTCTGTC CTGCCGCTGT GGCAGGAGTT CCAGCATCGG 
GTTACCAACT ACCAGCAGTT TTTGGGCCAC ATTGGCAACT GCCTAATGTA CTTGCTCTAC 
GATGTGCCCA TTCCGCAGGC CGTAAAGGGG GGAGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163323.1
CDS	30..2048
Translation	MANSKRKLPTKPSATEDATPHKDTPRSSEEFPTDVNHKSAKKSFLPQNAFFRDCLGHDLSNFTSLTNFTSWLNRPVDGAALGIFRLLYGAAMLIDIAEERGGGQLDVRFQEPLHCHFPLFNGMRALDYPLMGCVYLCMWLGAWGIMLGYRFRTSCLAFLIPYWYIFLLDKPTWNNHSYLFGLVGTLLLFTQAESYCSLDRWLNPRKSHCIPYWNYFLIKFQFFILYMYAGLKKFSLEWLSGYAMSNLSYHWVFAPFRQVIDPELIDLLIVHWFTAFFDVSIAFFMTLEKTRLLVTPFMLSFHLMNSRLFVIGMFPWVCLAEVPLFFSFDWPRRLGNWSKIQDVHPKDPEEELSKPGILARLRTGLILAYCGLQLFLPYSHFITKGYNNWTNGLYGYSWDMMVHSYDTLQTSIQVVDNESRQVHNLNPYAFTEYDRWTKYADMAVQYARCIEENLQEKQPHIGRNISIYFDIWCSMNGRFQQRSFDPREDLLRAKWSPFESTSWSLPLLNELNHMRPKLRTIETEVLAWNNYSDVIFVADFPGLTLTNFISPDLINCTLTILEGNVRYRNERDPEAYFLTAGKSIGLESNITHLVTTIGQKPASYLFTYTNKTMLEQGITIEQPDTEERSVLPLWQEFQHRVTNYQQFLGHIGNCLMYLLYDVPIPQAVKGGD

Target ORF information:

RefSeq Version	NM_001169852.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster gamma-glutamyl carboxylase (GC), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169852.1

ORF Insert Sequence:

ATGGCCAACT CCAAGCGAAA GCTGCCCACG AAACCCAGTG CCACAGAGGA CGCTACTCCG 
CACAAAGATA CACCTAGGTC GTCGGAAGAA TTCCCCACAG ATGTAAATCA CAAGAGTGCC 
AAGAAGTCCT TCCTTCCTCA AAACGCATTC TTTCGAGATT GCCTCGGTCA CGATCTGAGC 
AACTTCACTA GCCTGACCAA CTTTACCAGC TGGCTAAACA GACCCGTAGA TGGAGCTGCC 
CTGGGCATCT TCCGTCTGCT CTACGGAGCA GCCATGCTGA TTGACATCGC AGAGGAGCGA 
GGTGGCGGCC AGCTGGACGT GCGGTTTCAG GAGCCGCTAC ACTGCCACTT TCCACTGTTC 
AACGGGATGC GAGCATTGGA CTATCCACTG ATGGGCTGCG TCTATCTGTG CATGTGGCTG 
GGCGCGTGGG GCATCATGCT GGGCTATCGA TTCAGGACTA GCTGCCTGGC CTTCCTGATA 
CCCTACTGGT ACATCTTCCT GCTGGACAAA CCGACGTGGA ATAACCACAG CTATCTATTC 
GGCCTGGTGG GAACATTGCT GCTCTTCACC CAAGCGGAGT CCTACTGCTC CCTGGACAGA 
TGGCTAAATC CCCGAAAGAG TCACTGTATT CCCTACTGGA ACTACTTCCT AATCAAATTC 
CAGTTCTTCA TCCTGTACAT GTACGCAGGT CTGAAGAAAT TCTCGCTGGA ATGGCTATCG 
GGATACGCGA TGTCCAATCT GAGTTACCAC TGGGTCTTTG CCCCGTTCCG GCAAGTGATT 
GATCCGGAGC TGATAGACCT GCTCATCGTG CATTGGTTCA CCGCCTTCTT CGACGTGTCC 
ATTGCTTTTT TCATGACTCT GGAAAAGACG CGCCTACTGG TCACTCCTTT CATGCTCAGT 
TTTCATCTAA TGAACTCCCG ACTGTTTGTA ATAGGCATGT TTCCCTGGGT GTGTCTGGCC 
GAAGTGCCGC TCTTCTTCAG CTTCGACTGG CCGAGGAGGC TAGGCAACTG GTCCAAAATA 
CAGGACGTCC ATCCAAAAGA TCCAGAGGAA GAGCTTTCCA AGCCGGGAAT CCTGGCCAGA 
CTGCGCACGG GCCTCATCCT TGCCTACTGT GGACTGCAAC TCTTCCTGCC ATACTCACAC 
TTCATCACCA AGGGCTATAA CAACTGGACG AATGGGCTGT ACGGCTACTC ATGGGACATG 
ATGGTGCACT CCTACGACAC GCTGCAGACC TCCATTCAGG TGGTGGACAA CGAGAGCCGC 
CAAGTGCACA ACCTGAATCC ATACGCCTTT ACGGAGTACG ACCGCTGGAC GAAGTACGCC 
GACATGGCAG TGCAGTATGC CAGGTGCATC GAGGAGAATT TACAAGAGAA ACAGCCACAT 
ATTGGGAGGA ACATTTCCAT CTACTTCGAC ATCTGGTGCT CCATGAACGG ACGCTTTCAG 
CAACGATCAT TCGATCCACG CGAGGATCTC CTCCGGGCCA AGTGGTCGCC CTTTGAGAGC 
ACCTCCTGGT CGCTGCCTCT GCTGAACGAG CTGAACCACA TGCGGCCCAA ATTGCGCACC 
ATTGAGACTG AGGTGCTGGC CTGGAACAAC TACTCCGATG TCATTTTCGT GGCGGATTTC 
CCGGGACTAA CTCTGACCAA CTTCATCTCG CCGGACCTTA TCAACTGCAC GCTGACCATT 
CTTGAGGGAA ATGTTCGGTA CCGGAACGAG CGGGATCCGG AGGCCTATTT CCTGACAGCC 
GGCAAGAGCA TCGGTCTGGA GAGCAACATC ACCCACTTGG TCACCACCAT TGGCCAGAAG 
CCAGCTTCGT ATCTGTTCAC ATACACCAAC AAAACGATGC TGGAGCAGGG CATCACGATA 
GAACAGCCTG ATACCGAGGA GCGGTCTGTC CTGCCGCTGT GGCAGGAGTT CCAGCATCGG 
GTTACCAACT ACCAGCAGTT TTTGGGCCAC ATTGGCAACT GCCTAATGTA CTTGCTCTAC 
GATGTGCCCA TTCCGCAGGC CGTAAAGGGG GGAGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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GC cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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