Ggcx cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ggcx
Entrez Gene ID	81716
Full Name	gamma-glutamyl carboxylase
General protein information	Preferred Names gamma-glutamyl carboxylase Names vitamin K-dependent gamma-carboxylase peptidyl-glutamate 4-carboxylase vitamin K gamma glutamyl carboxylase vitamin K-dependent gamma-glutamyl carboxylase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q31
Summary	catalyzes the posttranslational modification of glutamate to gamma-carboxyglutamate (Gla); may mediate functions of vitamin K-dependent proteins (VKDPs) [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017592910.1	XP_017448399.1	vitamin K-dependent gamma-carboxylase isoform X2
XM_017592909.1	XP_017448398.1	vitamin K-dependent gamma-carboxylase isoform X1
NM_031756.1	NP_113944.1	vitamin K-dependent gamma-carboxylase

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GGCX	XP_515586.2
Canis lupus familiaris (dog)	GGCX	XP_532979.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006363	XP_316389.4
Homo sapiens (human)	GGCX	NP_000812.2
	GGCX	XP_001086474.1
Bos taurus (cattle)	GGCX	NP_776491.1
Mus musculus (house mouse)	Ggcx	NP_062776.1
Rattus norvegicus (Norway rat)	Ggcx	NP_113944.1
Xenopus tropicalis (tropical clawed frog)	ggcx	NP_001116905.1
Danio rerio (zebrafish)	LOC100537604	XP_003199342.1
Drosophila melanogaster (fruit fly)	GC	NP_001163323.1

Gene Ontology
Bibliography

Related articles in PubMed

Vitamin K Metabolism in a Rat Model of Chronic Kidney Disease.
McCabe KM, Booth SL, Fu X, Ward E, Adams MA, Holden RM
American journal of nephrology45(1)4-13(2017)

Effect of gamma-carboxylase inhibition on serum osteocalcin may be partially protective against developing diabetic cardiomyopathy in type 2 diabetic rats.
Gamal SM, Sadek NB, Rashed LA, Shawky HM, Gamal El-Din MM
Diabetes & vascular disease research13(6)405-417(2016 11)

Structural and functional insights into enzymes of the vitamin K cycle.
Tie JK, Stafford DW
Journal of thrombosis and haemostasis : JTH14(2)236-47(2016 Feb)

Liver-specific ?-glutamyl carboxylase-deficient mice display bleeding diathesis and short life span.
Azuma K, Tsukui T, Ikeda K, Shiba S, Nakagawa K, Okano T, Urano T, Horie-Inoue K, Ouchi Y, Ikawa M, Inoue S
PloS one9(2)e88643(2014)

Decreased expression of ?-carboxylase in diabetes-associated arterial stiffness: impact on matrix Gla protein.
Doyon M, Mathieu P, Moreau P
Cardiovascular research97(2)331-8(2013 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In a rat model of chronic kidney disease, there was a decrease in the thoracic aorta expression of Vkor and Ggcx enzymes, and a decrease in the kidney level of vitamin K1 to MK-4 bioconversion enzyme Ubiad1.
Title: Vitamin K Metabolism in a Rat Model of Chronic Kidney Disease.

gamma-Carboxylase inhibition prevents osteocalcin carboxylation and protects against diabetic cardiomyopathy, possibly through direct action on upregulated G protein-coupled receptor family C group 6 member A and indirectly via adiponectin.
Title: Effect of gamma-carboxylase inhibition on serum osteocalcin may be partially protective against developing diabetic cardiomyopathy in type 2 diabetic rats.

reduced gamma-Carboxylated MGP (cMGP), through an impaired expression of gamma-carboxylase, is involved in the early development of Medial artery calcification (MAC)in diabetes.
Title: Decreased expression of ?-carboxylase in diabetes-associated arterial stiffness: impact on matrix Gla protein.

Both carboxylase activity and formation of gamma-carboxyglutamate (Gla)-containing proteins were stimulated by dexamethasone (Dex) in fetal type II lung cells
Title: Glucocorticoid effects on vitamin K-dependent carboxylase activity and matrix Gla protein expression in rat lung.

The following Ggcx gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ggcx cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa61018	XM_017592910.1 Latest version!	Rattus norvegicus gamma-glutamyl carboxylase (Ggcx), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa61017	XM_017592909.1 Latest version!	Rattus norvegicus gamma-glutamyl carboxylase (Ggcx), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa08662	NM_031756.1 Latest version!	Rattus norvegicus gamma-glutamyl carboxylase (Ggcx), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$447.30 $639.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ggcx ( XM_017592910.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448399.1 Rattus norvegicus gamma-glutamyl carboxylase (Ggcx), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa61018
Clone ID Related Accession (Same CDS sequence)	XM_017592910.1
Accession Version	XM_017592910.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	vitamin K-dependent gamma-carboxylase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTCC ACCGTGGCTC TGCACGAGCT GCTCCCGCCT CAGATAAAGT ACAGAAAAAC 
AAGCCTGCAC AGACATCGGG GCTGAAACAG GGCAGCCGAA TGGCGAAAAT TTTTGGGTTT 
GAATGGGCAG ATTTATCCAG CTGGCAGAGT GTCGTGACCC TACTTAACCG ACCAACGGAC 
CCTGCAAACC TGGCTGTCTT CCGTTTTCTC TTTGCATTCT TGATGCTGCT GGACATCCCC 
CAGGAACGGG GCCTTAGCTC CCTGGACCGA AAATACTTGG ATGGGCTGGA TGTGTGCCGC 
TTCCCCTTGC TGGATGCCTT GCGCCCACTG CCACTTGACT GGATGTATCT CGTCTACACC 
ATCATGTTTC TGGGGGCACT GGGCATGATG CTGGGCCTGT GGTACCGGCT AAGCTGTGTG 
TTATTCCTGC TACCATACTG GTACGTGTTT CTCCTGGACA AGACATCGTG GAACAATCAC 
TCCTATCTGT ATGGTTTGTT GGCCTTTCAG TTGACATTCA TGGATGCAAA CCACTACTGG 
TACCCTGATA GATCCACTCC TGAGAAGACT AAAGCCAAAC ATAGCTGTTT TCAGATACTC 
CAGGGGTGCA GACTTTACTT TCTGTGTGTG AGCCCAGAGA GGCAGAATTA TAGCCAAACA 
CAGAAGTTGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448399.1
CDS	114..788
Translation	MAVHRGSARAAPASDKVQKNKPAQTSGLKQGSRMAKIFGFEWADLSSWQSVVTLLNRPTDPANLAVFRFLFAFLMLLDIPQERGLSSLDRKYLDGLDVCRFPLLDALRPLPLDWMYLVYTIMFLGALGMMLGLWYRLSCVLFLLPYWYVFLLDKTSWNNHSYLYGLLAFQLTFMDANHYWYPDRSTPEKTKAKHSCFQILQGCRLYFLCVSPERQNYSQTQKLL

Target ORF information:

RefSeq Version	XM_017592910.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus gamma-glutamyl carboxylase (Ggcx), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592910.1

ORF Insert Sequence:

ATGGCTGTCC ACCGTGGCTC TGCACGAGCT GCTCCCGCCT CAGATAAAGT ACAGAAAAAC 
AAGCCTGCAC AGACATCGGG GCTGAAACAG GGCAGCCGAA TGGCGAAAAT TTTTGGGTTT 
GAATGGGCAG ATTTATCCAG CTGGCAGAGT GTCGTGACCC TACTTAACCG ACCAACGGAC 
CCTGCAAACC TGGCTGTCTT CCGTTTTCTC TTTGCATTCT TGATGCTGCT GGACATCCCC 
CAGGAACGGG GCCTTAGCTC CCTGGACCGA AAATACTTGG ATGGGCTGGA TGTGTGCCGC 
TTCCCCTTGC TGGATGCCTT GCGCCCACTG CCACTTGACT GGATGTATCT CGTCTACACC 
ATCATGTTTC TGGGGGCACT GGGCATGATG CTGGGCCTGT GGTACCGGCT AAGCTGTGTG 
TTATTCCTGC TACCATACTG GTACGTGTTT CTCCTGGACA AGACATCGTG GAACAATCAC 
TCCTATCTGT ATGGTTTGTT GGCCTTTCAG TTGACATTCA TGGATGCAAA CCACTACTGG 
TACCCTGATA GATCCACTCC TGAGAAGACT AAAGCCAAAC ATAGCTGTTT TCAGATACTC 
CAGGGGTGCA GACTTTACTT TCTGTGTGTG AGCCCAGAGA GGCAGAATTA TAGCCAAACA 
CAGAAGTTGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ggcx ( XM_017592909.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448398.1 Rattus norvegicus gamma-glutamyl carboxylase (Ggcx), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa61017
Clone ID Related Accession (Same CDS sequence)	XM_017592909.1
Accession Version	XM_017592909.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	vitamin K-dependent gamma-carboxylase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTCC ACCGTGGCTC TGCACGAGCT GCTCCCGCCT CAGATAAAGT ACAGAAAAAC 
AAGCCTGCAC AGACATCGGG GCTGAAACAG GGCAGCCGAA TGGCGAAAAT TTTTGGGTTT 
GAATGGGCAG ATTTATCCAG CTGGCAGAGT GTCGTGACCC TACTTAACCG ACCAACGGAC 
CCTGCAAACC TGGCTGTCTT CCGTTTTCTC TTTGCATTCT TGATGCTGCT GGACATCCCC 
CAGGAACGGG GCCTTAGCTC CCTGGACCGA AAATACTTGG ATGGGCTGGA TGTGTGCCGC 
TTCCCCTTGC TGGATGCCTT GCGCCCACTG CCACTTGACT GGATGTATCT CGTCTACACC 
ATCATGTTTC TGGGGGCACT GGGCATGATG CTGGGCCTGT GGTACCGGCT AAGCTGTGTG 
TTATTCCTGC TACCATACTG GTACGTGTTT CTCCTGGACA AGACATCGTG GAACAATCAC 
TCCTATCTGT ATGGTTTGTT GGCCTTTCAG TTGACATTCA TGGATGCAAA CCACTACTGG 
TCTGTGGATG GCCTGCTGAG TGCCCAAAAG AAGAATGCTC ACGTGCCCCT TTGGAACTAC 
ACAGTCCTGC GTGGCCAGAT CTTCATTGTG TATTTCATTG CGGGTGTGAA AAAGCTGGAT 
GCTGACTGGG TTGAGGGCTA CTCCATGGAG CATTTGTCCC GGCACTGGCT TTTTAGTCCC 
TTCAAGCTAG TGTTGTCGGA GGAGCTGACA AGCCTGCTGG TGGTACACTG GTGTGGGCTT 
CTCCTTGACC TCTCGGCTGG CTTCCTGCTC TTCTTCGATG CCTCCAGACC CATTGGCCTC 
GTCTTCGTGT CCTACTTTCA CTGCATGAAC TCGCAGCTCT TCAGTATAGG TATGTTCCCC 
TATGTCATGC TGGCCAGCAG CCCTCTCTTC TGCTCAGCTG AATGGCCTCG GAAGCTGGTA 
GCCCGATGCC CGAAAAGGCT GCAAGAGCTG CTGCCTGCCA AAGCTGCTCC TCGGCCTAGT 
GCTTCCTGTG TGTATAAGAG GGCCCGGGCG AAAGCTGGTC AAAAGCCAGG CCTACGCCAC 
CATCTGGGAG CTGTCTTCAC CCTGCTCTAC CTTCTGGAGC AGCTCTTCCT GCCCTATTCC 
CATTTCCTCA CGCAGGGTTA CAACAACTGG ACGAATGGGC TGTATGGCTA TTCCTGGGAC 
ATGATGGTGC ACTCCCGCTC CCACCAGCAT GTAAAGATCA CCTACCGTGA TGGCCTCACT 
GGAGAGCTGG GCTACCTTAA CCCTGGGGCT TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448398.1
CDS	114..1409
Translation	MAVHRGSARAAPASDKVQKNKPAQTSGLKQGSRMAKIFGFEWADLSSWQSVVTLLNRPTDPANLAVFRFLFAFLMLLDIPQERGLSSLDRKYLDGLDVCRFPLLDALRPLPLDWMYLVYTIMFLGALGMMLGLWYRLSCVLFLLPYWYVFLLDKTSWNNHSYLYGLLAFQLTFMDANHYWSVDGLLSAQKKNAHVPLWNYTVLRGQIFIVYFIAGVKKLDADWVEGYSMEHLSRHWLFSPFKLVLSEELTSLLVVHWCGLLLDLSAGFLLFFDASRPIGLVFVSYFHCMNSQLFSIGMFPYVMLASSPLFCSAEWPRKLVARCPKRLQELLPAKAAPRPSASCVYKRARAKAGQKPGLRHHLGAVFTLLYLLEQLFLPYSHFLTQGYNNWTNGLYGYSWDMMVHSRSHQHVKITYRDGLTGELGYLNPGAF

Target ORF information:

RefSeq Version	XM_017592909.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus gamma-glutamyl carboxylase (Ggcx), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592909.1

ORF Insert Sequence:

ATGGCTGTCC ACCGTGGCTC TGCACGAGCT GCTCCCGCCT CAGATAAAGT ACAGAAAAAC 
AAGCCTGCAC AGACATCGGG GCTGAAACAG GGCAGCCGAA TGGCGAAAAT TTTTGGGTTT 
GAATGGGCAG ATTTATCCAG CTGGCAGAGT GTCGTGACCC TACTTAACCG ACCAACGGAC 
CCTGCAAACC TGGCTGTCTT CCGTTTTCTC TTTGCATTCT TGATGCTGCT GGACATCCCC 
CAGGAACGGG GCCTTAGCTC CCTGGACCGA AAATACTTGG ATGGGCTGGA TGTGTGCCGC 
TTCCCCTTGC TGGATGCCTT GCGCCCACTG CCACTTGACT GGATGTATCT CGTCTACACC 
ATCATGTTTC TGGGGGCACT GGGCATGATG CTGGGCCTGT GGTACCGGCT AAGCTGTGTG 
TTATTCCTGC TACCATACTG GTACGTGTTT CTCCTGGACA AGACATCGTG GAACAATCAC 
TCCTATCTGT ATGGTTTGTT GGCCTTTCAG TTGACATTCA TGGATGCAAA CCACTACTGG 
TCTGTGGATG GCCTGCTGAG TGCCCAAAAG AAGAATGCTC ACGTGCCCCT TTGGAACTAC 
ACAGTCCTGC GTGGCCAGAT CTTCATTGTG TATTTCATTG CGGGTGTGAA AAAGCTGGAT 
GCTGACTGGG TTGAGGGCTA CTCCATGGAG CATTTGTCCC GGCACTGGCT TTTTAGTCCC 
TTCAAGCTAG TGTTGTCGGA GGAGCTGACA AGCCTGCTGG TGGTACACTG GTGTGGGCTT 
CTCCTTGACC TCTCGGCTGG CTTCCTGCTC TTCTTCGATG CCTCCAGACC CATTGGCCTC 
GTCTTCGTGT CCTACTTTCA CTGCATGAAC TCGCAGCTCT TCAGTATAGG TATGTTCCCC 
TATGTCATGC TGGCCAGCAG CCCTCTCTTC TGCTCAGCTG AATGGCCTCG GAAGCTGGTA 
GCCCGATGCC CGAAAAGGCT GCAAGAGCTG CTGCCTGCCA AAGCTGCTCC TCGGCCTAGT 
GCTTCCTGTG TGTATAAGAG GGCCCGGGCG AAAGCTGGTC AAAAGCCAGG CCTACGCCAC 
CATCTGGGAG CTGTCTTCAC CCTGCTCTAC CTTCTGGAGC AGCTCTTCCT GCCCTATTCC 
CATTTCCTCA CGCAGGGTTA CAACAACTGG ACGAATGGGC TGTATGGCTA TTCCTGGGAC 
ATGATGGTGC ACTCCCGCTC CCACCAGCAT GTAAAGATCA CCTACCGTGA TGGCCTCACT 
GGAGAGCTGG GCTACCTTAA CCCTGGGGCT TTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ggcx ( NM_031756.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113944.1 Rattus norvegicus gamma-glutamyl carboxylase (Ggcx), mRNA.

Price & Availability↑

CloneID	ORa08662
Clone ID Related Accession (Same CDS sequence)	NM_031756.1
Accession Version	NM_031756.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	vitamin K-dependent gamma-carboxylase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF065387.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF065387.1, BC168647.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGTCC ACCGTGGCTC TGCACGAGCT GCTCCCGCCT CAGATAAAGT ACAGAAAAAC 
AAGCCTGCAC AGACATCGGG GCTGGAACAG GGCAGCCGAA TGGCGAGAAT TTTTGGGTTT 
GAATGGGCAG ATTTATCCAG CTGGCAGAGT GTCGTGACCC TACTTAACCG ACCAACGGAC 
CCTGCAAACC TGGCTGTCTT CCGTTTTCTC TTTGCATTCT TGATGCTGCT GGACATCCCC 
CAGGAACGGG GCCTTAGCTC CCTGGACCGA AAATACTTGG ATGGGCTGGA TGTGTGCCGC 
TTCCCCTTGC TGGATGCCTT GCGCCCACTG CCACTTGACT GGATGTATCT CGTCTACACC 
ATCATGTTTC TGGGGGCACT GGGCATGATG CTGGGCCTGT GGTACCGGCT AAGCTGTATG 
TTATTCCTGC TACCATACTG GTACGTGTTT CTCCTGGACA AGACATCGTG GAACAATCAC 
TCCTATCTGT ATGGTTTGTT GGCCTTTCAG TTGACATTCA TGGATGCAAA CCACTACTGG 
TCTGTGGATG GCCTGCTGAG TGCCCAAAAG AAGAATGCTC ACGTGCCCCT TTGGAACTAC 
ACAGTCCTGC GTGGCCAGAT CTTCATTGTG TATTTCATTG CGGGTGTGAA AAAGCTGGAT 
GCTGACTGGG TTGAGGGCTA CTCCATGGAG CATTTGTCCC GGCACTGGCT TTTTAGTCCC 
TTCAAGCTAG TGTTGTCGGA GGAGCTGACA AGCCTGCTGG TGGTACACTG GTGTGGGCTT 
CTCCTTGACC TCTCGGCTGG CTTCCTGCTC TTCTTCGATG CCTCCAGACC CATTGGCCTC 
GTCTTCGTGT CCTACTTTCA CTGCATGAAC TCGCAGCTCT TCAGTATAGG TATGTTCCCC 
TATGTCATGC TGGCCAGCAG CCCTCTCTTC TGCTCAGCTG AATGGCCTCG GAAGCTGGTA 
GCCCGATGCC CGAAAAGGCT GCAAGAGCTG CTGCCTGCCA AAGCTGCTCC TCGGCCTAGT 
GCTTCCTGTG TGTATAAGAG GGCCCGGGCG AAAGCTGGTC AAAAGCCAGG CCTACGCCAC 
CATCTGGGAA CTGTCTTCAC CCTGCTCTAC CTTCTGGAGC AGCTCTTCCT GCCCTATTCC 
CATTTCCTCA CGCAGGGTTA CAACAACTGG ACGAATGGGC TGTATGGCTA TTCCTGGGAC 
ATGATGGTGC ACTCCCGCTC CCACCAGCAT GTAAAGATCA CCTACCGTGA TGGCCTCACT 
GGAGAGCTGG GCTACCTTAA CCCTGGGGTA TTCACACAGA GCCGGCGCTG GAAGGACCAT 
GCAGACATGC TGAAGCAATA TGCCACTTGC CTGAGCCTCC TGCTTCCCAA GTACAATGTC 
ACTGAGCCCC AGATCTACTT TGATATCTGG GTCTCCATCA ATGACCGCTT CCAGCAGAGG 
CTTTTTGACC CTCGCGTGGA CATCGTGCAG GCCGTGTGGT CCCCCTTCCG GCGCACGCCT 
TGGGTGCAGC CACTCTTGAT GGACTTATCT CCCTGGAGGA CCAAGTTACA AGACATTAAG 
AGCAGTCTGG ACAACCACAC TGAGGTGGTC TTCATTGCAG ATTTCCCTGG GCTTCACTTG 
GAGAATTTTG TGAGTGAAGA CCTGGGCAAC ACCAGCATCC AGCTGCTGCA GGGAGAAGTC 
ACTGTGGAAT TGGTGGCAGA ACAGAAAAAC CAGACTCTTC GAGAAGGAGA GAAAATGCAG 
TTGCCTGCTG GAGAGTACCA TAAAGTCTAT ACTGTATCAT CTAGTCCTTC CTGCTACATG 
TACATCTATG TCAACACTAC AGAGGTCGCA CTGGAGCAAG ACCTAGCATA TCTGCAAGAA 
TTAAAGGAGA AGGTGGAGAA CGGAAGTGAA ACAGGGCCCC TGCCTCCAGA ACTTCAGCCT 
CTTTTGGAAG GGGAAGTAAA AGGGGGCCCT GAGCCAACAC CTCTGGTCCA AACCTTTCTG 
AGACGGCAGA GGAAGCTCCA AGAAATTGAA CGAAGGCGAA ATAGCCCTTT GCATGAGCGC 
TTCCTCCGCT TTGTGCTGCG GAAGCTGTAT GTCTTTCGAC GCAGCTTCCT GATGACTCGA 
ATTTCACTTC GAAACCTGCT ATTTGGCCGC CCTTCCCTAG AGCAATTAGC CCAAGAGGTG 
ACATATGCAA ACTTGCGACC ATTTGAACCA GTTGATGAGT CAAGTGCTTC AAACACAGAT 
TCTTCCGATC CTCATCCTTC AGAGCCAGAT TCTGAGCATG TTCACTCTGA GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113944.1
CDS	76..2352
Translation	MAVHRGSARAAPASDKVQKNKPAQTSGLEQGSRMARIFGFEWADLSSWQSVVTLLNRPTDPANLAVFRFLFAFLMLLDIPQERGLSSLDRKYLDGLDVCRFPLLDALRPLPLDWMYLVYTIMFLGALGMMLGLWYRLSCMLFLLPYWYVFLLDKTSWNNHSYLYGLLAFQLTFMDANHYWSVDGLLSAQKKNAHVPLWNYTVLRGQIFIVYFIAGVKKLDADWVEGYSMEHLSRHWLFSPFKLVLSEELTSLLVVHWCGLLLDLSAGFLLFFDASRPIGLVFVSYFHCMNSQLFSIGMFPYVMLASSPLFCSAEWPRKLVARCPKRLQELLPAKAAPRPSASCVYKRARAKAGQKPGLRHHLGTVFTLLYLLEQLFLPYSHFLTQGYNNWTNGLYGYSWDMMVHSRSHQHVKITYRDGLTGELGYLNPGVFTQSRRWKDHADMLKQYATCLSLLLPKYNVTEPQIYFDIWVSINDRFQQRLFDPRVDIVQAVWSPFRRTPWVQPLLMDLSPWRTKLQDIKSSLDNHTEVVFIADFPGLHLENFVSEDLGNTSIQLLQGEVTVELVAEQKNQTLREGEKMQLPAGEYHKVYTVSSSPSCYMYIYVNTTEVALEQDLAYLQELKEKVENGSETGPLPPELQPLLEGEVKGGPEPTPLVQTFLRRQRKLQEIERRRNSPLHERFLRFVLRKLYVFRRSFLMTRISLRNLLFGRPSLEQLAQEVTYANLRPFEPVDESSASNTDSSDPHPSEPDSEHVHSEL

Target ORF information:

RefSeq Version	NM_031756.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus gamma-glutamyl carboxylase (Ggcx), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031756.1

ORF Insert Sequence:

ATGGCTGTCC ACCGTGGCTC TGCACGAGCT GCTCCCGCCT CAGATAAAGT ACAGAAAAAC 
AAGCCTGCAC AGACATCGGG GCTGGAACAG GGCAGCCGAA TGGCGAGAAT TTTTGGGTTT 
GAATGGGCAG ATTTATCCAG CTGGCAGAGT GTCGTGACCC TACTTAACCG ACCAACGGAC 
CCTGCAAACC TGGCTGTCTT CCGTTTTCTC TTTGCATTCT TGATGCTGCT GGACATCCCC 
CAGGAACGGG GCCTTAGCTC CCTGGACCGA AAATACTTGG ATGGGCTGGA TGTGTGCCGC 
TTCCCCTTGC TGGATGCCTT GCGCCCACTG CCACTTGACT GGATGTATCT CGTCTACACC 
ATCATGTTTC TGGGGGCACT GGGCATGATG CTGGGCCTGT GGTACCGGCT AAGCTGTATG 
TTATTCCTGC TACCATACTG GTACGTGTTT CTCCTGGACA AGACATCGTG GAACAATCAC 
TCCTATCTGT ATGGTTTGTT GGCCTTTCAG TTGACATTCA TGGATGCAAA CCACTACTGG 
TCTGTGGATG GCCTGCTGAG TGCCCAAAAG AAGAATGCTC ACGTGCCCCT TTGGAACTAC 
ACAGTCCTGC GTGGCCAGAT CTTCATTGTG TATTTCATTG CGGGTGTGAA AAAGCTGGAT 
GCTGACTGGG TTGAGGGCTA CTCCATGGAG CATTTGTCCC GGCACTGGCT TTTTAGTCCC 
TTCAAGCTAG TGTTGTCGGA GGAGCTGACA AGCCTGCTGG TGGTACACTG GTGTGGGCTT 
CTCCTTGACC TCTCGGCTGG CTTCCTGCTC TTCTTCGATG CCTCCAGACC CATTGGCCTC 
GTCTTCGTGT CCTACTTTCA CTGCATGAAC TCGCAGCTCT TCAGTATAGG TATGTTCCCC 
TATGTCATGC TGGCCAGCAG CCCTCTCTTC TGCTCAGCTG AATGGCCTCG GAAGCTGGTA 
GCCCGATGCC CGAAAAGGCT GCAAGAGCTG CTGCCTGCCA AAGCTGCTCC TCGGCCTAGT 
GCTTCCTGTG TGTATAAGAG GGCCCGGGCG AAAGCTGGTC AAAAGCCAGG CCTACGCCAC 
CATCTGGGAA CTGTCTTCAC CCTGCTCTAC CTTCTGGAGC AGCTCTTCCT GCCCTATTCC 
CATTTCCTCA CGCAGGGTTA CAACAACTGG ACGAATGGGC TGTATGGCTA TTCCTGGGAC 
ATGATGGTGC ACTCCCGCTC CCACCAGCAT GTAAAGATCA CCTACCGTGA TGGCCTCACT 
GGAGAGCTGG GCTACCTTAA CCCTGGGGTA TTCACACAGA GCCGGCGCTG GAAGGACCAT 
GCAGACATGC TGAAGCAATA TGCCACTTGC CTGAGCCTCC TGCTTCCCAA GTACAATGTC 
ACTGAGCCCC AGATCTACTT TGATATCTGG GTCTCCATCA ATGACCGCTT CCAGCAGAGG 
CTTTTTGACC CTCGCGTGGA CATCGTGCAG GCCGTGTGGT CCCCCTTCCG GCGCACGCCT 
TGGGTGCAGC CACTCTTGAT GGACTTATCT CCCTGGAGGA CCAAGTTACA AGACATTAAG 
AGCAGTCTGG ACAACCACAC TGAGGTGGTC TTCATTGCAG ATTTCCCTGG GCTTCACTTG 
GAGAATTTTG TGAGTGAAGA CCTGGGCAAC ACCAGCATCC AGCTGCTGCA GGGAGAAGTC 
ACTGTGGAAT TGGTGGCAGA ACAGAAAAAC CAGACTCTTC GAGAAGGAGA GAAAATGCAG 
TTGCCTGCTG GAGAGTACCA TAAAGTCTAT ACTGTATCAT CTAGTCCTTC CTGCTACATG 
TACATCTATG TCAACACTAC AGAGGTCGCA CTGGAGCAAG ACCTAGCATA TCTGCAAGAA 
TTAAAGGAGA AGGTGGAGAA CGGAAGTGAA ACAGGGCCCC TGCCTCCAGA ACTTCAGCCT 
CTTTTGGAAG GGGAAGTAAA AGGGGGCCCT GAGCCAACAC CTCTGGTCCA AACCTTTCTG 
AGACGGCAGA GGAAGCTCCA AGAAATTGAA CGAAGGCGAA ATAGCCCTTT GCATGAGCGC 
TTCCTCCGCT TTGTGCTGCG GAAGCTGTAT GTCTTTCGAC GCAGCTTCCT GATGACTCGA 
ATTTCACTTC GAAACCTGCT ATTTGGCCGC CCTTCCCTAG AGCAATTAGC CCAAGAGGTG 
ACATATGCAA ACTTGCGACC ATTTGAACCA GTTGATGAGT CAAGTGCTTC AAACACAGAT 
TCTTCCGATC CTCATCCTTC AGAGCCAGAT TCTGAGCATG TTCACTCTGA GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Vitamin K Metabolism in a Rat Model of Chronic Kidney Disease.
American journal of nephrology45(1)4-13(2017)
McCabe KM,Booth SL,Fu X,Ward E,Adams MA,Holden RM

Effect of gamma-carboxylase inhibition on serum osteocalcin may be partially protective against developing diabetic cardiomyopathy in type 2 diabetic rats.
Diabetes & vascular disease research13(6)405-417(2016 11)
Gamal SM,Sadek NB,Rashed LA,Shawky HM,Gamal El-Din MM

Structural and functional insights into enzymes of the vitamin K cycle.
Journal of thrombosis and haemostasis : JTH14(2)236-47(2016 Feb)
Tie JK,Stafford DW

Liver-specific ?-glutamyl carboxylase-deficient mice display bleeding diathesis and short life span.
PloS one9(2)e88643(2014)
Azuma K,Tsukui T,Ikeda K,Shiba S,Nakagawa K,Okano T,Urano T,Horie-Inoue K,Ouchi Y,Ikawa M,Inoue S

Decreased expression of ?-carboxylase in diabetes-associated arterial stiffness: impact on matrix Gla protein.
Cardiovascular research97(2)331-8(2013 Feb)
Doyon M,Mathieu P,Moreau P