CG2277 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG2277
Entrez Gene ID	38148
Full Name	CG2277 gene product from transcript CG2277-RB
Synonyms	28575006,Dmel\CG2277
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 61F5-61F5

mRNA and Protein(s)

mRNA	Protein	Name
NM_138251.3	NP_612095.3	uncharacterized protein, isoform A
NM_001299968.1	NP_001286897.1	uncharacterized protein, isoform C
NM_001274319.1	NP_001261248.1	uncharacterized protein, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NT5DC1	XP_518703.2
Gallus gallus (chicken)	NT5DC1	XP_003641054.1
	NT5DC1	XP_001112048.1
Danio rerio (zebrafish)	nt5dc1	NP_001098413.1
Drosophila melanogaster (fruit fly)	CG2277	NP_001261248.1
Canis lupus familiaris (dog)	NT5DC1	XP_532271.2
Bos taurus (cattle)	NT5DC1	NP_001098955.1
Rattus norvegicus (Norway rat)	Nt5dc1	NP_001099863.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001319	XP_321827.5
Homo sapiens (human)	NT5DC1	NP_689942.2
Mus musculus (house mouse)	Nt5dc1	NP_795942.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG2277 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG2277 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17132	NM_001274319.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG2277), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa17132	NM_138251.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG2277), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa21970	NM_001299968.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG2277), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG2277 ( NM_138251.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_612095.3 Drosophila melanogaster uncharacterized protein, transcript variant A (CG2277), mRNA.

Price & Availability↑

CloneID	OFa17132
Clone ID Related Accession (Same CDS sequence)	NM_138251.3 , NM_001274319.1
Accession Version	NM_138251.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_138251.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGGCGC TGGGCAGAGC AGCCACCGTG GGCGCCTTTT CCGCCCCTGC CCTTTCACTT 
CGACTGGCCA CGAAAACGAA AACGAAAACA AAAACAAACG TACACAAACA GGCCCAAACA 
GATGTTCGTC TCCTGTTACG CAATCTCGAT AAATTGGCGC AAAATTCAAT GACGTTAGTC 
AGAACACCGC CGGCAGCAGT GGGAAGAGCA ACACGAGAAG TGACGGGTCA GCAGCAGACA 
GTTAGGCGCT TCCACAAATC TGCAGGGGGA AACTCAAGGT ATTCGGCCAG CACCATCATG 
ACATCCTATC ACAAGGAGCT GCTCCTAAAG GATTATGGAA TAGTGGGCTT CGACCTGGAC 
GGCACCCTTC TGCGCTACAA TCTCCACGAG ATGTCCGCTC TGATCTATCA GGTGCTCAAG 
CAGTTCCTCG TAGAGCAGAA GGGCTATGAC AGGGATCTAC TCCTTCAATC GCTCAACTTG 
GACTTTCTGC AGAAGGGCCT GTTCCTGGAC GGACCGCGTG GCAATGTGCT GAAGCTGAGC 
AGTGAGGCCC AGATCCTGCG AGCCACCCAT GGCACCCGTC TACTAAGCGA CACAGAAATT 
CAAGCCATCT ATGGAAGAGA TCGAAGGTGG GAAATCACCA CTGCCTTCTA CAGGGACCCC 
CTGTCCACCT GGAATGGCGA CGCCTCGACG CAAATGCGCT CGCTATTGGA TTACTTTGAT 
ATGCCCAGTG CCTTGGTCTT TGCCCAGGCG GTGGATCTGG TCGATAGGGG AAAGAAGCCA 
TCGGCAGGAG AGGAGTATCA GGTCTGGCAA GATGTTCAGG CTGGCCTGAT GAACATCTAC 
AGTCGGGAGA ACTTCGCCAA CGACCAGAGT CTCTATTTCA AGAGCATGCG AGCGGAACCG 
GAACGATTTG TGCTCCGAAC AGATCCCAGT GTGATGAACT GGCTCGGGGA ACTGCGAAAA 
TCCGGCAAGA AGCTATTTTT GCTCACCGGC TCCAACGTGG ACTTCGCCAA TTTGACGGCC 
ACGCAGGCGC TGGGCAAGGA TTGGCGCAAA TACTTCGATT TCGTGGTAAC ATACGCCAAG 
AAACCAGGGT TCTTTACCAT GCAGCGTCCG TTCATGCAGG TGGATGTGGA AAAACTGGCA 
GAACTGCCGC AAGAGGCAGA CAACATAAAG GCGGGCGAGG TCTACTCGCA AGGCAACTGG 
CACCAGCTGC ACACTGCCAT GGCTGGAATG CTCCAGAAGA AGCCCTCTGA AGCCAAGGCA 
CTATACTTCG GTGATAACAT AATCCAGGAT GTTTACACAC CGGTGAAACA CCGCGACTTC 
GATGCCGTGG CCATAGCCGA GGAGCTGCTC CTTGAGGATG GTAAATATCC GTATGCTGCC 
GAATTGGACT CCCAGCTGTG GGGACCATAC TTTGCCCTGG ACTGCACACC AACTCTGTGG 
TCCAGCTTCA TCAGCAGCTA TGCCCAGTTG TGCGTGCCCA CGGTGGAGCA AGTGGCGCAG 
ACTGCGCCAG ATAAGCGAAT CGTTTGCCAC AATCCGTATG GCTTCAGTCC GCGGCTGCCC 
AAGGAGCTGG AGACTGTGCA GCTGAAGAGC TGCTGGCGTG GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612095.3
CDS	131..1735
Translation	MLALGRAATVGAFSAPALSLRLATKTKTKTKTNVHKQAQTDVRLLLRNLDKLAQNSMTLVRTPPAAVGRATREVTGQQQTVRRFHKSAGGNSRYSASTIMTSYHKELLLKDYGIVGFDLDGTLLRYNLHEMSALIYQVLKQFLVEQKGYDRDLLLQSLNLDFLQKGLFLDGPRGNVLKLSSEAQILRATHGTRLLSDTEIQAIYGRDRRWEITTAFYRDPLSTWNGDASTQMRSLLDYFDMPSALVFAQAVDLVDRGKKPSAGEEYQVWQDVQAGLMNIYSRENFANDQSLYFKSMRAEPERFVLRTDPSVMNWLGELRKSGKKLFLLTGSNVDFANLTATQALGKDWRKYFDFVVTYAKKPGFFTMQRPFMQVDVEKLAELPQEADNIKAGEVYSQGNWHQLHTAMAGMLQKKPSEAKALYFGDNIIQDVYTPVKHRDFDAVAIAEELLLEDGKYPYAAELDSQLWGPYFALDCTPTLWSSFISSYAQLCVPTVEQVAQTAPDKRIVCHNPYGFSPRLPKELETVQLKSCWRG

Target ORF information:

RefSeq Version	NM_138251.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG2277), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138251.3

ORF Insert Sequence:

ATGCTGGCGC TGGGCAGAGC AGCCACCGTG GGCGCCTTTT CCGCCCCTGC CCTTTCACTT 
CGACTGGCCA CGAAAACGAA AACGAAAACA AAAACAAACG TACACAAACA GGCCCAAACA 
GATGTTCGTC TCCTGTTACG CAATCTCGAT AAATTGGCGC AAAATTCAAT GACGTTAGTC 
AGAACACCGC CGGCAGCAGT GGGAAGAGCA ACACGAGAAG TGACGGGTCA GCAGCAGACA 
GTTAGGCGCT TCCACAAATC TGCAGGGGGA AACTCAAGGT ATTCGGCCAG CACCATCATG 
ACATCCTATC ACAAGGAGCT GCTCCTAAAG GATTATGGAA TAGTGGGCTT CGACCTGGAC 
GGCACCCTTC TGCGCTACAA TCTCCACGAG ATGTCCGCTC TGATCTATCA GGTGCTCAAG 
CAGTTCCTCG TAGAGCAGAA GGGCTATGAC AGGGATCTAC TCCTTCAATC GCTCAACTTG 
GACTTTCTGC AGAAGGGCCT GTTCCTGGAC GGACCGCGTG GCAATGTGCT GAAGCTGAGC 
AGTGAGGCCC AGATCCTGCG AGCCACCCAT GGCACCCGTC TACTAAGCGA CACAGAAATT 
CAAGCCATCT ATGGAAGAGA TCGAAGGTGG GAAATCACCA CTGCCTTCTA CAGGGACCCC 
CTGTCCACCT GGAATGGCGA CGCCTCGACG CAAATGCGCT CGCTATTGGA TTACTTTGAT 
ATGCCCAGTG CCTTGGTCTT TGCCCAGGCG GTGGATCTGG TCGATAGGGG AAAGAAGCCA 
TCGGCAGGAG AGGAGTATCA GGTCTGGCAA GATGTTCAGG CTGGCCTGAT GAACATCTAC 
AGTCGGGAGA ACTTCGCCAA CGACCAGAGT CTCTATTTCA AGAGCATGCG AGCGGAACCG 
GAACGATTTG TGCTCCGAAC AGATCCCAGT GTGATGAACT GGCTCGGGGA ACTGCGAAAA 
TCCGGCAAGA AGCTATTTTT GCTCACCGGC TCCAACGTGG ACTTCGCCAA TTTGACGGCC 
ACGCAGGCGC TGGGCAAGGA TTGGCGCAAA TACTTCGATT TCGTGGTAAC ATACGCCAAG 
AAACCAGGGT TCTTTACCAT GCAGCGTCCG TTCATGCAGG TGGATGTGGA AAAACTGGCA 
GAACTGCCGC AAGAGGCAGA CAACATAAAG GCGGGCGAGG TCTACTCGCA AGGCAACTGG 
CACCAGCTGC ACACTGCCAT GGCTGGAATG CTCCAGAAGA AGCCCTCTGA AGCCAAGGCA 
CTATACTTCG GTGATAACAT AATCCAGGAT GTTTACACAC CGGTGAAACA CCGCGACTTC 
GATGCCGTGG CCATAGCCGA GGAGCTGCTC CTTGAGGATG GTAAATATCC GTATGCTGCC 
GAATTGGACT CCCAGCTGTG GGGACCATAC TTTGCCCTGG ACTGCACACC AACTCTGTGG 
TCCAGCTTCA TCAGCAGCTA TGCCCAGTTG TGCGTGCCCA CGGTGGAGCA AGTGGCGCAG 
ACTGCGCCAG ATAAGCGAAT CGTTTGCCAC AATCCGTATG GCTTCAGTCC GCGGCTGCCC 
AAGGAGCTGG AGACTGTGCA GCTGAAGAGC TGCTGGCGTG GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG2277 ( NM_001274319.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261248.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG2277), mRNA.

Price & Availability↑

CloneID	OFa17132
Clone ID Related Accession (Same CDS sequence)	NM_138251.3 , NM_001274319.1
Accession Version	NM_001274319.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGGCGC TGGGCAGAGC AGCCACCGTG GGCGCCTTTT CCGCCCCTGC CCTTTCACTT 
CGACTGGCCA CGAAAACGAA AACGAAAACA AAAACAAACG TACACAAACA GGCCCAAACA 
GATGTTCGTC TCCTGTTACG CAATCTCGAT AAATTGGCGC AAAATTCAAT GACGTTAGTC 
AGAACACCGC CGGCAGCAGT GGGAAGAGCA ACACGAGAAG TGACGGGTCA GCAGCAGACA 
GTTAGGCGCT TCCACAAATC TGCAGGGGGA AACTCAAGGT ATTCGGCCAG CACCATCATG 
ACATCCTATC ACAAGGAGCT GCTCCTAAAG GATTATGGAA TAGTGGGCTT CGACCTGGAC 
GGCACCCTTC TGCGCTACAA TCTCCACGAG ATGTCCGCTC TGATCTATCA GGTGCTCAAG 
CAGTTCCTCG TAGAGCAGAA GGGCTATGAC AGGGATCTAC TCCTTCAATC GCTCAACTTG 
GACTTTCTGC AGAAGGGCCT GTTCCTGGAC GGACCGCGTG GCAATGTGCT GAAGCTGAGC 
AGTGAGGCCC AGATCCTGCG AGCCACCCAT GGCACCCGTC TACTAAGCGA CACAGAAATT 
CAAGCCATCT ATGGAAGAGA TCGAAGGTGG GAAATCACCA CTGCCTTCTA CAGGGACCCC 
CTGTCCACCT GGAATGGCGA CGCCTCGACG CAAATGCGCT CGCTATTGGA TTACTTTGAT 
ATGCCCAGTG CCTTGGTCTT TGCCCAGGCG GTGGATCTGG TCGATAGGGG AAAGAAGCCA 
TCGGCAGGAG AGGAGTATCA GGTCTGGCAA GATGTTCAGG CTGGCCTGAT GAACATCTAC 
AGTCGGGAGA ACTTCGCCAA CGACCAGAGT CTCTATTTCA AGAGCATGCG AGCGGAACCG 
GAACGATTTG TGCTCCGAAC AGATCCCAGT GTGATGAACT GGCTCGGGGA ACTGCGAAAA 
TCCGGCAAGA AGCTATTTTT GCTCACCGGC TCCAACGTGG ACTTCGCCAA TTTGACGGCC 
ACGCAGGCGC TGGGCAAGGA TTGGCGCAAA TACTTCGATT TCGTGGTAAC ATACGCCAAG 
AAACCAGGGT TCTTTACCAT GCAGCGTCCG TTCATGCAGG TGGATGTGGA AAAACTGGCA 
GAACTGCCGC AAGAGGCAGA CAACATAAAG GCGGGCGAGG TCTACTCGCA AGGCAACTGG 
CACCAGCTGC ACACTGCCAT GGCTGGAATG CTCCAGAAGA AGCCCTCTGA AGCCAAGGCA 
CTATACTTCG GTGATAACAT AATCCAGGAT GTTTACACAC CGGTGAAACA CCGCGACTTC 
GATGCCGTGG CCATAGCCGA GGAGCTGCTC CTTGAGGATG GTAAATATCC GTATGCTGCC 
GAATTGGACT CCCAGCTGTG GGGACCATAC TTTGCCCTGG ACTGCACACC AACTCTGTGG 
TCCAGCTTCA TCAGCAGCTA TGCCCAGTTG TGCGTGCCCA CGGTGGAGCA AGTGGCGCAG 
ACTGCGCCAG ATAAGCGAAT CGTTTGCCAC AATCCGTATG GCTTCAGTCC GCGGCTGCCC 
AAGGAGCTGG AGACTGTGCA GCTGAAGAGC TGCTGGCGTG GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261248.1
CDS	131..1735
Translation	MLALGRAATVGAFSAPALSLRLATKTKTKTKTNVHKQAQTDVRLLLRNLDKLAQNSMTLVRTPPAAVGRATREVTGQQQTVRRFHKSAGGNSRYSASTIMTSYHKELLLKDYGIVGFDLDGTLLRYNLHEMSALIYQVLKQFLVEQKGYDRDLLLQSLNLDFLQKGLFLDGPRGNVLKLSSEAQILRATHGTRLLSDTEIQAIYGRDRRWEITTAFYRDPLSTWNGDASTQMRSLLDYFDMPSALVFAQAVDLVDRGKKPSAGEEYQVWQDVQAGLMNIYSRENFANDQSLYFKSMRAEPERFVLRTDPSVMNWLGELRKSGKKLFLLTGSNVDFANLTATQALGKDWRKYFDFVVTYAKKPGFFTMQRPFMQVDVEKLAELPQEADNIKAGEVYSQGNWHQLHTAMAGMLQKKPSEAKALYFGDNIIQDVYTPVKHRDFDAVAIAEELLLEDGKYPYAAELDSQLWGPYFALDCTPTLWSSFISSYAQLCVPTVEQVAQTAPDKRIVCHNPYGFSPRLPKELETVQLKSCWRG

Target ORF information:

RefSeq Version	NM_001274319.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG2277), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274319.1

ORF Insert Sequence:

ATGCTGGCGC TGGGCAGAGC AGCCACCGTG GGCGCCTTTT CCGCCCCTGC CCTTTCACTT 
CGACTGGCCA CGAAAACGAA AACGAAAACA AAAACAAACG TACACAAACA GGCCCAAACA 
GATGTTCGTC TCCTGTTACG CAATCTCGAT AAATTGGCGC AAAATTCAAT GACGTTAGTC 
AGAACACCGC CGGCAGCAGT GGGAAGAGCA ACACGAGAAG TGACGGGTCA GCAGCAGACA 
GTTAGGCGCT TCCACAAATC TGCAGGGGGA AACTCAAGGT ATTCGGCCAG CACCATCATG 
ACATCCTATC ACAAGGAGCT GCTCCTAAAG GATTATGGAA TAGTGGGCTT CGACCTGGAC 
GGCACCCTTC TGCGCTACAA TCTCCACGAG ATGTCCGCTC TGATCTATCA GGTGCTCAAG 
CAGTTCCTCG TAGAGCAGAA GGGCTATGAC AGGGATCTAC TCCTTCAATC GCTCAACTTG 
GACTTTCTGC AGAAGGGCCT GTTCCTGGAC GGACCGCGTG GCAATGTGCT GAAGCTGAGC 
AGTGAGGCCC AGATCCTGCG AGCCACCCAT GGCACCCGTC TACTAAGCGA CACAGAAATT 
CAAGCCATCT ATGGAAGAGA TCGAAGGTGG GAAATCACCA CTGCCTTCTA CAGGGACCCC 
CTGTCCACCT GGAATGGCGA CGCCTCGACG CAAATGCGCT CGCTATTGGA TTACTTTGAT 
ATGCCCAGTG CCTTGGTCTT TGCCCAGGCG GTGGATCTGG TCGATAGGGG AAAGAAGCCA 
TCGGCAGGAG AGGAGTATCA GGTCTGGCAA GATGTTCAGG CTGGCCTGAT GAACATCTAC 
AGTCGGGAGA ACTTCGCCAA CGACCAGAGT CTCTATTTCA AGAGCATGCG AGCGGAACCG 
GAACGATTTG TGCTCCGAAC AGATCCCAGT GTGATGAACT GGCTCGGGGA ACTGCGAAAA 
TCCGGCAAGA AGCTATTTTT GCTCACCGGC TCCAACGTGG ACTTCGCCAA TTTGACGGCC 
ACGCAGGCGC TGGGCAAGGA TTGGCGCAAA TACTTCGATT TCGTGGTAAC ATACGCCAAG 
AAACCAGGGT TCTTTACCAT GCAGCGTCCG TTCATGCAGG TGGATGTGGA AAAACTGGCA 
GAACTGCCGC AAGAGGCAGA CAACATAAAG GCGGGCGAGG TCTACTCGCA AGGCAACTGG 
CACCAGCTGC ACACTGCCAT GGCTGGAATG CTCCAGAAGA AGCCCTCTGA AGCCAAGGCA 
CTATACTTCG GTGATAACAT AATCCAGGAT GTTTACACAC CGGTGAAACA CCGCGACTTC 
GATGCCGTGG CCATAGCCGA GGAGCTGCTC CTTGAGGATG GTAAATATCC GTATGCTGCC 
GAATTGGACT CCCAGCTGTG GGGACCATAC TTTGCCCTGG ACTGCACACC AACTCTGTGG 
TCCAGCTTCA TCAGCAGCTA TGCCCAGTTG TGCGTGCCCA CGGTGGAGCA AGTGGCGCAG 
ACTGCGCCAG ATAAGCGAAT CGTTTGCCAC AATCCGTATG GCTTCAGTCC GCGGCTGCCC 
AAGGAGCTGG AGACTGTGCA GCTGAAGAGC TGCTGGCGTG GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG2277 ( NM_001299968.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286897.1 Drosophila melanogaster uncharacterized protein, transcript variant C (CG2277), mRNA.

Price & Availability↑

CloneID	OFa21970
Clone ID Related Accession (Same CDS sequence)	NM_001299968.1
Accession Version	NM_001299968.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACATCCT ATCACAAGGA GCTGCTCCTA AAGGATTATG GAATAGTGGG CTTCGACCTG 
GACGGCACCC TTCTGCGCTA CAATCTCCAC GAGATGTCCG CTCTGATCTA TCAGGTGCTC 
AAGCAGTTCC TCGTAGAGCA GAAGGGCTAT GACAGGGATC TACTCCTTCA ATCGCTCAAC 
TTGGACTTTC TGCAGAAGGG CCTGTTCCTG GACGGACCGC GTGGCAATGT GCTGAAGCTG 
AGCAGTGAGG CCCAGATCCT GCGAGCCACC CATGGCACCC GTCTACTAAG CGACACAGAA 
ATTCAAGCCA TCTATGGAAG AGATCGAAGG TGGGAAATCA CCACTGCCTT CTACAGGGAC 
CCCCTGTCCA CCTGGAATGG CGACGCCTCG ACGCAAATGC GCTCGCTATT GGATTACTTT 
GATATGCCCA GTGCCTTGGT CTTTGCCCAG GCGGTGGATC TGGTCGATAG GGGAAAGAAG 
CCATCGGCAG GAGAGGAGTA TCAGGTCTGG CAAGATGTTC AGGCTGGCCT GATGAACATC 
TACAGTCGGG AGAACTTCGC CAACGACCAG AGTCTCTATT TCAAGAGCAT GCGAGCGGAA 
CCGGAACGAT TTGTGCTCCG AACAGATCCC AGTGTGATGA ACTGGCTCGG GGAACTGCGA 
AAATCCGGCA AGAAGCTATT TTTGCTCACC GGCTCCAACG TGGACTTCGC CAATTTGACG 
GCCACGCAGG CGCTGGGCAA GGATTGGCGC AAATACTTCG ATTTCGTGGT AACATACGCC 
AAGAAACCAG GGTTCTTTAC CATGCAGCGT CCGTTCATGC AGGTGGATGT GGAAAAACTG 
GCAGAACTGC CGCAAGAGGC AGACAACATA AAGGCGGGCG AGGTCTACTC GCAAGGCAAC 
TGGCACCAGC TGCACACTGC CATGGCTGGA ATGCTCCAGA AGAAGCCCTC TGAAGCCAAG 
GCACTATACT TCGGTGATAA CATAATCCAG GATGTTTACA CACCGGTGAA ACACCGCGAC 
TTCGATGCCG TGGCCATAGC CGAGGAGCTG CTCCTTGAGG ATGGTAAATA TCCGTATGCT 
GCCGAATTGG ACTCCCAGCT GTGGGGACCA TACTTTGCCC TGGACTGCAC ACCAACTCTG 
TGGTCCAGCT TCATCAGCAG CTATGCCCAG TTGTGCGTGC CCACGGTGGA GCAAGTGGCG 
CAGACTGCGC CAGATAAGCG AATCGTTTGC CACAATCCGT ATGGCTTCAG TCCGCGGCTG 
CCCAAGGAGC TGGAGACTGT GCAGCTGAAG AGCTGCTGGC GTGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286897.1
CDS	122..1429
Translation	MTSYHKELLLKDYGIVGFDLDGTLLRYNLHEMSALIYQVLKQFLVEQKGYDRDLLLQSLNLDFLQKGLFLDGPRGNVLKLSSEAQILRATHGTRLLSDTEIQAIYGRDRRWEITTAFYRDPLSTWNGDASTQMRSLLDYFDMPSALVFAQAVDLVDRGKKPSAGEEYQVWQDVQAGLMNIYSRENFANDQSLYFKSMRAEPERFVLRTDPSVMNWLGELRKSGKKLFLLTGSNVDFANLTATQALGKDWRKYFDFVVTYAKKPGFFTMQRPFMQVDVEKLAELPQEADNIKAGEVYSQGNWHQLHTAMAGMLQKKPSEAKALYFGDNIIQDVYTPVKHRDFDAVAIAEELLLEDGKYPYAAELDSQLWGPYFALDCTPTLWSSFISSYAQLCVPTVEQVAQTAPDKRIVCHNPYGFSPRLPKELETVQLKSCWRG

Target ORF information:

RefSeq Version	NM_001299968.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG2277), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299968.1

ORF Insert Sequence:

ATGACATCCT ATCACAAGGA GCTGCTCCTA AAGGATTATG GAATAGTGGG CTTCGACCTG 
GACGGCACCC TTCTGCGCTA CAATCTCCAC GAGATGTCCG CTCTGATCTA TCAGGTGCTC 
AAGCAGTTCC TCGTAGAGCA GAAGGGCTAT GACAGGGATC TACTCCTTCA ATCGCTCAAC 
TTGGACTTTC TGCAGAAGGG CCTGTTCCTG GACGGACCGC GTGGCAATGT GCTGAAGCTG 
AGCAGTGAGG CCCAGATCCT GCGAGCCACC CATGGCACCC GTCTACTAAG CGACACAGAA 
ATTCAAGCCA TCTATGGAAG AGATCGAAGG TGGGAAATCA CCACTGCCTT CTACAGGGAC 
CCCCTGTCCA CCTGGAATGG CGACGCCTCG ACGCAAATGC GCTCGCTATT GGATTACTTT 
GATATGCCCA GTGCCTTGGT CTTTGCCCAG GCGGTGGATC TGGTCGATAG GGGAAAGAAG 
CCATCGGCAG GAGAGGAGTA TCAGGTCTGG CAAGATGTTC AGGCTGGCCT GATGAACATC 
TACAGTCGGG AGAACTTCGC CAACGACCAG AGTCTCTATT TCAAGAGCAT GCGAGCGGAA 
CCGGAACGAT TTGTGCTCCG AACAGATCCC AGTGTGATGA ACTGGCTCGG GGAACTGCGA 
AAATCCGGCA AGAAGCTATT TTTGCTCACC GGCTCCAACG TGGACTTCGC CAATTTGACG 
GCCACGCAGG CGCTGGGCAA GGATTGGCGC AAATACTTCG ATTTCGTGGT AACATACGCC 
AAGAAACCAG GGTTCTTTAC CATGCAGCGT CCGTTCATGC AGGTGGATGT GGAAAAACTG 
GCAGAACTGC CGCAAGAGGC AGACAACATA AAGGCGGGCG AGGTCTACTC GCAAGGCAAC 
TGGCACCAGC TGCACACTGC CATGGCTGGA ATGCTCCAGA AGAAGCCCTC TGAAGCCAAG 
GCACTATACT TCGGTGATAA CATAATCCAG GATGTTTACA CACCGGTGAA ACACCGCGAC 
TTCGATGCCG TGGCCATAGC CGAGGAGCTG CTCCTTGAGG ATGGTAAATA TCCGTATGCT 
GCCGAATTGG ACTCCCAGCT GTGGGGACCA TACTTTGCCC TGGACTGCAC ACCAACTCTG 
TGGTCCAGCT TCATCAGCAG CTATGCCCAG TTGTGCGTGC CCACGGTGGA GCAAGTGGCG 
CAGACTGCGC CAGATAAGCG AATCGTTTGC CACAATCCGT ATGGCTTCAG TCCGCGGCTG 
CCCAAGGAGC TGGAGACTGT GCAGCTGAAG AGCTGCTGGC GTGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG2277 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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