wac cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	wac
Entrez Gene ID	38044
Full Name	wee Augmin
Synonyms	CG13879,Dgt8,Dmel\CG13879,Wac,was
General protein information	Preferred Names wee Augmin Names CG13879 gene product from transcript CG13879-RD CG13879-PB CG13879-PC CG13879-PD augmin wac-PB wac-PC wac-PD wee augmin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 61B3-61B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_138176.3	NP_612020.2	wee augmin, isoform B
NM_001169835.1	NP_001163306.1	wee augmin, isoform C
NM_001274265.1	NP_001261194.1	wee augmin, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

WAC promotes the interaction between TTT and Pontin/Reptin in an energy-dependent manner, thereby promoting mTORC1 activity by facilitating mTORC1 dimerization and mTORC1-Rag interaction
Title: WAC Regulates mTOR Activity by Acting as an Adaptor for the TTT and Pontin/Reptin Complexes.

Results show that Augmin and gamma-tubulin are required for microtubule nucleation and acentrosomal spindle formation.
Title: Differing requirements for Augmin in male meiotic and mitotic spindle formation in Drosophila.

meiosis-specific regulation of Augmin compensates for the lack of centrosomes in oocytes by actively biasing sites of microtubule generation within the spindle.
Title: Meiosis-specific stable binding of augmin to acentrosomal spindle poles promotes biased microtubule assembly in oocytes.

It mediates mitotic spindle formation. (review)
Title: [Mitotic spindle formation mediated by augmin protein complex].

The following wac gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wac cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17049	NM_001274265.1 Latest version!	Drosophila melanogaster wee augmin, transcript variant D (wac), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa17049	NM_138176.3 Latest version!	Drosophila melanogaster wee augmin, transcript variant B (wac), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa21185	NM_001169835.1 Latest version!	Drosophila melanogaster wee augmin, transcript variant C (wac), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wac ( NM_138176.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_612020.2 Drosophila melanogaster wee augmin, transcript variant B (wac), mRNA.

Price & Availability↑

CloneID	OFa17049
Clone ID Related Accession (Same CDS sequence)	NM_138176.3 , NM_001274265.1
Accession Version	NM_138176.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wee augmin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Dec 17, 2009 this sequence version replaced NM_138176.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGAACT TGAAGATCCA AGAGGAAGTC AATTCATTGA TGCGTCTAGG CCAGCACTTC 
GATGACCAGT TAAAGCTGGC TAGCGTCGAG TTGGGCGATT TCTCGGACGA TGACCTGGCG 
CTCCTTGACA AATGCGCCCA ATATTATTCA CTTTTGCACA TTCACGACAT CAATCTGAAC 
TACTTGCGCG ACTTCTATTG TGCCAAGAAG AGGGAATGCA TAGAAAACCG GCAGACCACA 
GTGCAGCAAC GCGTAGAGCT GCAGCGCATC CTCTCGTCCA TCGAGGAGGC GACTAGGGAC 
GTAGTTATGT TGGAGAGATT TAATGCTGCT GCGGAAGAGC GGCTCATCCC GGATATCGTC 
GTAATGCAGC GAAACGCCCA ACAGCTTGCA ACAAAACAGG CCTTACTGGA CCGGCAAAAA 
ACCCTTAAGA TCCCAAAGGA TTTTAGCATT GAAAGTGTCA TCGAGAAGGT AGATTCGCTG 
GAGCAGCGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612020.2
CDS	43..534
Translation	MQNLKIQEEVNSLMRLGQHFDDQLKLASVELGDFSDDDLALLDKCAQYYSLLHIHDINLNYLRDFYCAKKRECIENRQTTVQQRVELQRILSSIEEATRDVVMLERFNAAAEERLIPDIVVMQRNAQQLATKQALLDRQKTLKIPKDFSIESVIEKVDSLEQR

Target ORF information:

RefSeq Version	NM_138176.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wee augmin, transcript variant B (wac), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138176.3

ORF Insert Sequence:

ATGCAGAACT TGAAGATCCA AGAGGAAGTC AATTCATTGA TGCGTCTAGG CCAGCACTTC 
GATGACCAGT TAAAGCTGGC TAGCGTCGAG TTGGGCGATT TCTCGGACGA TGACCTGGCG 
CTCCTTGACA AATGCGCCCA ATATTATTCA CTTTTGCACA TTCACGACAT CAATCTGAAC 
TACTTGCGCG ACTTCTATTG TGCCAAGAAG AGGGAATGCA TAGAAAACCG GCAGACCACA 
GTGCAGCAAC GCGTAGAGCT GCAGCGCATC CTCTCGTCCA TCGAGGAGGC GACTAGGGAC 
GTAGTTATGT TGGAGAGATT TAATGCTGCT GCGGAAGAGC GGCTCATCCC GGATATCGTC 
GTAATGCAGC GAAACGCCCA ACAGCTTGCA ACAAAACAGG CCTTACTGGA CCGGCAAAAA 
ACCCTTAAGA TCCCAAAGGA TTTTAGCATT GAAAGTGTCA TCGAGAAGGT AGATTCGCTG 
GAGCAGCGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wac ( NM_001274265.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261194.1 Drosophila melanogaster wee augmin, transcript variant D (wac), mRNA.

Price & Availability↑

CloneID	OFa17049
Clone ID Related Accession (Same CDS sequence)	NM_138176.3 , NM_001274265.1
Accession Version	NM_001274265.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wee augmin, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGAACT TGAAGATCCA AGAGGAAGTC AATTCATTGA TGCGTCTAGG CCAGCACTTC 
GATGACCAGT TAAAGCTGGC TAGCGTCGAG TTGGGCGATT TCTCGGACGA TGACCTGGCG 
CTCCTTGACA AATGCGCCCA ATATTATTCA CTTTTGCACA TTCACGACAT CAATCTGAAC 
TACTTGCGCG ACTTCTATTG TGCCAAGAAG AGGGAATGCA TAGAAAACCG GCAGACCACA 
GTGCAGCAAC GCGTAGAGCT GCAGCGCATC CTCTCGTCCA TCGAGGAGGC GACTAGGGAC 
GTAGTTATGT TGGAGAGATT TAATGCTGCT GCGGAAGAGC GGCTCATCCC GGATATCGTC 
GTAATGCAGC GAAACGCCCA ACAGCTTGCA ACAAAACAGG CCTTACTGGA CCGGCAAAAA 
ACCCTTAAGA TCCCAAAGGA TTTTAGCATT GAAAGTGTCA TCGAGAAGGT AGATTCGCTG 
GAGCAGCGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261194.1
CDS	217..708
Translation	MQNLKIQEEVNSLMRLGQHFDDQLKLASVELGDFSDDDLALLDKCAQYYSLLHIHDINLNYLRDFYCAKKRECIENRQTTVQQRVELQRILSSIEEATRDVVMLERFNAAAEERLIPDIVVMQRNAQQLATKQALLDRQKTLKIPKDFSIESVIEKVDSLEQR

Target ORF information:

RefSeq Version	NM_001274265.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wee augmin, transcript variant D (wac), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274265.1

ORF Insert Sequence:

ATGCAGAACT TGAAGATCCA AGAGGAAGTC AATTCATTGA TGCGTCTAGG CCAGCACTTC 
GATGACCAGT TAAAGCTGGC TAGCGTCGAG TTGGGCGATT TCTCGGACGA TGACCTGGCG 
CTCCTTGACA AATGCGCCCA ATATTATTCA CTTTTGCACA TTCACGACAT CAATCTGAAC 
TACTTGCGCG ACTTCTATTG TGCCAAGAAG AGGGAATGCA TAGAAAACCG GCAGACCACA 
GTGCAGCAAC GCGTAGAGCT GCAGCGCATC CTCTCGTCCA TCGAGGAGGC GACTAGGGAC 
GTAGTTATGT TGGAGAGATT TAATGCTGCT GCGGAAGAGC GGCTCATCCC GGATATCGTC 
GTAATGCAGC GAAACGCCCA ACAGCTTGCA ACAAAACAGG CCTTACTGGA CCGGCAAAAA 
ACCCTTAAGA TCCCAAAGGA TTTTAGCATT GAAAGTGTCA TCGAGAAGGT AGATTCGCTG 
GAGCAGCGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wac ( NM_001169835.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163306.1 Drosophila melanogaster wee augmin, transcript variant C (wac), mRNA.

Price & Availability↑

CloneID	OFa21185
Clone ID Related Accession (Same CDS sequence)	NM_001169835.1
Accession Version	NM_001169835.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wee augmin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGTCTAG GCCAGCACTT CGATGACCAG TTAAAGCTGG CTAGCGTCGA GTTGGGCGAT 
TTCTCGGACG ATGACCTGGC GCTCCTTGAC AAATGCGCCC AATATTATTC ACTTTTGCAC 
ATTCACGACA TCAATCTGAA CTACTTGCGC GACTTCTATT GTGCCAAGAA GAGGGAATGC 
ATAGAAAACC GGCAGACCAC AGTGCAGCAA CGCGTAGAGC TGCAGCGCAT CCTCTCGTCC 
ATCGAGGAGG CGACTAGGGA CGTAGTTATG TTGGAGAGAT TTAATGCTGC TGCGGAAGAG 
CGGCTCATCC CGGATATCGT CGTAATGCAG CGAAACGCCC AACAGCTTGC AACAAAACAG 
GCCTTACTGG ACCGGCAAAA AACCCTTAAG ATCCCAAAGG ATTTTAGCAT TGAAAGTGTC 
ATCGAGAAGG TAGATTCGCT GGAGCAGCGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163306.1
CDS	81..533
Translation	MRLGQHFDDQLKLASVELGDFSDDDLALLDKCAQYYSLLHIHDINLNYLRDFYCAKKRECIENRQTTVQQRVELQRILSSIEEATRDVVMLERFNAAAEERLIPDIVVMQRNAQQLATKQALLDRQKTLKIPKDFSIESVIEKVDSLEQR

Target ORF information:

RefSeq Version	NM_001169835.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wee augmin, transcript variant C (wac), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169835.1

ORF Insert Sequence:

ATGCGTCTAG GCCAGCACTT CGATGACCAG TTAAAGCTGG CTAGCGTCGA GTTGGGCGAT 
TTCTCGGACG ATGACCTGGC GCTCCTTGAC AAATGCGCCC AATATTATTC ACTTTTGCAC 
ATTCACGACA TCAATCTGAA CTACTTGCGC GACTTCTATT GTGCCAAGAA GAGGGAATGC 
ATAGAAAACC GGCAGACCAC AGTGCAGCAA CGCGTAGAGC TGCAGCGCAT CCTCTCGTCC 
ATCGAGGAGG CGACTAGGGA CGTAGTTATG TTGGAGAGAT TTAATGCTGC TGCGGAAGAG 
CGGCTCATCC CGGATATCGT CGTAATGCAG CGAAACGCCC AACAGCTTGC AACAAAACAG 
GCCTTACTGG ACCGGCAAAA AACCCTTAAG ATCCCAAAGG ATTTTAGCAT TGAAAGTGTC 
ATCGAGAAGG TAGATTCGCT GGAGCAGCGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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wac cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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