Gp150 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gp150
Entrez Gene ID	37549
Full Name	CG5820 gene product from transcript CG5820-RC
Synonyms	CG5820,CT18251,Dmel\CG5820,GP150,anon-EST:fe2A1,gp150,l(2)52A,p150
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 58D3-58D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001299785.1	NP_001286714.1	Gp150, isoform F
NM_166528.2	NP_726191.1	Gp150, isoform B
NM_166529.2	NP_726192.1	Gp150, isoform C
NM_166527.2	NP_726190.1	Gp150, isoform A
NM_001299784.1	NP_001286713.1	Gp150, isoform E
NM_057701.4	NP_477049.2	Gp150, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that endosomal Scabrous and Gp150 promote Notch activation in response to Delta, by regulating acquisition of insensitivity to Delta in a subset of cells.
Title: Scabrous and Gp150 are endosomal proteins that regulate Notch activity.

Drosophila Gp150 is required for early ommatidial development through modulation of Notch signaling. Loss of gp150 function causes accumulation of intracellular Delta protein.
Title: Drosophila Gp150 is required for early ommatidial development through modulation of Notch signaling.

The following Gp150 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gp150 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10912	NM_166529.2 Latest version!	Drosophila melanogaster Gp150, transcript variant C (Gp150), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa10912	NM_166527.2 Latest version!	Drosophila melanogaster Gp150, transcript variant A (Gp150), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa10912	NM_001299785.1 Latest version!	Drosophila melanogaster Gp150, transcript variant F (Gp150), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa10912	NM_166528.2 Latest version!	Drosophila melanogaster Gp150, transcript variant B (Gp150), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa10913	NM_057701.4 Latest version!	Drosophila melanogaster Gp150, transcript variant D (Gp150), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa21911	NM_001299784.1 Latest version!	Drosophila melanogaster Gp150, transcript variant E (Gp150), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gp150 ( NM_001299785.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286714.1 Drosophila melanogaster Gp150, transcript variant F (Gp150), mRNA.

Price & Availability↑

CloneID	OFa10912
Clone ID Related Accession (Same CDS sequence)	NM_001299785.1 , NM_166528.2 , NM_166529.2 , NM_166527.2
Accession Version	NM_001299785.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Gp150, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTCGCC AGTCGACTTG CAGGCTAGTC CTGCTGCTGG CCTTTACCCT GGCCCTAATC 
CACGCCAGCC ACTCAAATAC CGTGGGCCCC AAGATCCACT TCATTCATGA CGATCTGACG 
GAAAACGAGC TGAACTCTGG AGAGGAGGTG AGCCCGACCG AGAGTCTGCC CAAAATCTCC 
ACCCCCAAGG TAAAGGATTT ACCCGTGGAG ACGACCACCA GGAGCCCCAC AAAAAAAACC 
ACGTCCTCCG GCACCGAGAA GTCCCTTAGC GAGGAGGCTT GGAATGATCT GATGGAGGCG 
GCCAAGCACA GCCAAGCTTT GGCTGATGAT CCACACTTCA ACAAGATGCT CAATACTACC 
GTCGCCGCCG GGGAGAAGAA CTCCACCGAT ACCGACGATA GCGAATACTA TGACTACGAT 
GATGATTACA ACTATTTCGA TGAGGAGACG GTGACGATTT CCCCCAGTCC CAAGAAGGCC 
AAGAGCAACC CCAAGCTGCA GCCCATTGTG GATGCCACCA TCACGAAGTC CAAGAGCAAA 
TCCAACGACC AAAAGACCAA AGAGAGTGCT AACGATATCG ACAGCGACTC TCAATATGAC 
TACGACGAGG GTCTGGATGA TGATGATGAC GAGGACGATG CCGCTGACGA CCTTTTGACC 
GATGATGAGC AAGTTGTCTT CAGCGAGGAC GTTCCGTGTC CAAGATTCTG CCAGTGTGCC 
CGGAATGTCA ACAGCTATCT GGTGGCCACC TGCAGCAGAC TTGACATGGG CATCCAGAAG 
TTTGGCTCTG ACATTACGGA TTTGGTTGTG ACCAATGTGG GTCCTAAGTA CCCCATCCTG 
ATGGGACCCA ACTTCTTCCA GAATCTGGGT CTTAAGAACG TGGCTTCCAT CAAGATCGCC 
AACTGCACCT TGGAGTACCT GCACGCCGAG GCCTTCCACG GACTGAACGA GCTGTACGCT 
GTTAACTTGA CGGATGTTGG TTTGGCTATC ATTAATCCCG ATACCTTTGT TGGAAACAAG 
AAACTGCGCA TGCTGACCAT TTCGGGTAAC GACTTGAGTG TGATGTCCAG CATTCACTAT 
TTGCTGAAGT CTTCCAGCAT TGAGGAGCTG GACTTCTCGC GCAACAACCT TATGGAGTTG 
AATCCCAAGG CCTTCTCCCA TCTATCCAAT GTCGTCTACA TTAACTTGAG CCAGAACAGT 
CTCAAGAAAC TGCCCGAGAA GGCTTTCGAG AAGGTTACTC TTCTGGAAGA ATTGGATCTG 
TCTTACAACT CGCTGACTGA ATTGCCCCGC GATATCTTCA ATGGCACCAC CCTGTCCATA 
CTGCACCTGA AGTACAATAC CTTCAATGGC GATCTGCACT TTGGCACCAA GGATCTGCAG 
CAGCTGGACT TGAGCTTTAA CTCGATTGTC CAAGTGCACC ACAGCATGTT CGACAAGATG 
CCCGGTCTGA CCAACCTGAA CCTCAAGGGC AATGGCATTA AGAAGATCCA ACCGGACTCG 
TTCCTTACTT TGAAGAACCT GCGCCACATC GATTTGTCCA TCAACGATCT GGACCAGATC 
TCGGGAATGC TGTTCTTTAA GAACTCTGAG CTGGATGTGA TCCGTTTGAA CGACAATCCT 
CGTCTGAGCC AGTTGCCCAC CGATGGCTTC TTGAGCTACA GTGGGGAGTT CACCGTCTAC 
TACCTGGATA TTTCCAATTG CGCCATCGGT CCATTGGGTC ACAAGGCCTT CTCCACTATG 
CCGCATTTGA CCACCTTGAA GTTGGCCTGG AACAACATTA ATCATCTGCC GCGCGAGATT 
TTCACTGGAC TGCACAAGCT GATTGACTTG GATCTGAGCA ACAACCTGAT CACCCGCATG 
GACGATCTGA TCTTCATGGA CAACGGAGAG CTGACTAAGC TCAGTCTGGC TGGAAATCCC 
ATTAGCCGCT TGTCCGTCCG CCTCTTCCTG CCGCTGCACC AACTGCGATG CCTGGACGTC 
AATGACTGCG AGCTTACCAC CTTGCTCTCC GATCGCGACT TGGGTGCGGG TTACAAGATC 
TTTGACAGCC TGCGCTCCTT TAACGCCTCT GGTAATTTGA TCAAGAAGAT CTCTTCGGAG 
GATGTGAAGA GCTTCAAGAA CCTGCGTTCT CTGGATATCA CCAACAATCC GCTGAAGTGC 
ACTCCCGACT TCCAGGAGTT CATCAGCTAC GTCACTCTTC AGATGCAGAT GACTCCCAAG 
CGTCTGCCCG TGCTGGCCAA TCTGGAGGAC GATGCTACTA TTGTGCAGCT GGAGACTCTG 
GCTCAGGCTG GTTGGTCGTC TTTGGCCCAC GAGGTGTGCA AGCATGCCGA AGGATCAGAT 
CTCTTGGACG AAAAGAAGGC CGACAGTGCC GAAGCCAAAC TGGAGAAGCG TCTGAAGGAA 
AGCGTGAAGA AGCTTAACGA GGATGAGGCT AAGTTGCTGA GTGTCCTGGA CAAGAGTGTG 
CTAGACAAGA GCAGGCTGAG CAGCATGCAG AAGATCATGA AGGGATCGGT GAACACTGAA 
ACCGCGAAGC CTGAGGAAGA TGCTGACGAG GAGAATCTGA ACAATGGCGG CGAGGATAAG 
GAGGATGACA GCAGTGACTA TGACAGTGAG GAGGATGATG ATGACGATGA TGAGGACGAT 
GATGATGACA ACAACGATGA TGATCGCTTC GAGGAGGCCA AGAAGAATGC CAAGGCTAAG 
GCCGATGCTA ACAAGGCCTT CGACACGTTC GTGAACAAGG AACTGAAGCC AGTCCTGAAG 
ACCGATGGTA TCCAGGCGGA GGAAGTGGAC ATGTCTCAGG AGACCCGTGA TAAATTCCTT 
CTGGAGAAAC TGGGCTTCGA CAGCGAAAGC GAGGAGTCAG ATGAGTACAC CGAGAAGATG 
ATCTTCCACG GCGAACTATC CTACGACCTG GTGGTCCCAC TGATCGTCGT GTCCTTCTGC 
CTCCTGATGA TCGTGCTGTC CATCGCCCGA GTGATCTACG TGGTGCTGCG CAAGCGCGGC 
GAGCGCTATC GCATGGCGCT GCTGGCCTCA AAGAACTCGT TCGTATACCA GAAGTTGAGC 
GAGGACATCG TGAAGCCGAC CAGCAAGGAG CAGAAGGAGC CCAAGCATCT GAAGCAGCCA 
AAGGTGCACC GATACGCGCC CATCAATCAG GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286714.1
CDS	77..3232
Translation	MSRQSTCRLVLLLAFTLALIHASHSNTVGPKIHFIHDDLTENELNSGEEVSPTESLPKISTPKVKDLPVETTTRSPTKKTTSSGTEKSLSEEAWNDLMEAAKHSQALADDPHFNKMLNTTVAAGEKNSTDTDDSEYYDYDDDYNYFDEETVTISPSPKKAKSNPKLQPIVDATITKSKSKSNDQKTKESANDIDSDSQYDYDEGLDDDDDEDDAADDLLTDDEQVVFSEDVPCPRFCQCARNVNSYLVATCSRLDMGIQKFGSDITDLVVTNVGPKYPILMGPNFFQNLGLKNVASIKIANCTLEYLHAEAFHGLNELYAVNLTDVGLAIINPDTFVGNKKLRMLTISGNDLSVMSSIHYLLKSSSIEELDFSRNNLMELNPKAFSHLSNVVYINLSQNSLKKLPEKAFEKVTLLEELDLSYNSLTELPRDIFNGTTLSILHLKYNTFNGDLHFGTKDLQQLDLSFNSIVQVHHSMFDKMPGLTNLNLKGNGIKKIQPDSFLTLKNLRHIDLSINDLDQISGMLFFKNSELDVIRLNDNPRLSQLPTDGFLSYSGEFTVYYLDISNCAIGPLGHKAFSTMPHLTTLKLAWNNINHLPREIFTGLHKLIDLDLSNNLITRMDDLIFMDNGELTKLSLAGNPISRLSVRLFLPLHQLRCLDVNDCELTTLLSDRDLGAGYKIFDSLRSFNASGNLIKKISSEDVKSFKNLRSLDITNNPLKCTPDFQEFISYVTLQMQMTPKRLPVLANLEDDATIVQLETLAQAGWSSLAHEVCKHAEGSDLLDEKKADSAEAKLEKRLKESVKKLNEDEAKLLSVLDKSVLDKSRLSSMQKIMKGSVNTETAKPEEDADEENLNNGGEDKEDDSSDYDSEEDDDDDDEDDDDDNNDDDRFEEAKKNAKAKADANKAFDTFVNKELKPVLKTDGIQAEEVDMSQETRDKFLLEKLGFDSESEESDEYTEKMIFHGELSYDLVVPLIVVSFCLLMIVLSIARVIYVVLRKRGERYRMALLASKNSFVYQKLSEDIVKPTSKEQKEPKHLKQPKVHRYAPINQV

Target ORF information:

RefSeq Version	NM_001299785.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Gp150, transcript variant F (Gp150), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299785.1

ORF Insert Sequence:

ATGAGTCGCC AGTCGACTTG CAGGCTAGTC CTGCTGCTGG CCTTTACCCT GGCCCTAATC 
CACGCCAGCC ACTCAAATAC CGTGGGCCCC AAGATCCACT TCATTCATGA CGATCTGACG 
GAAAACGAGC TGAACTCTGG AGAGGAGGTG AGCCCGACCG AGAGTCTGCC CAAAATCTCC 
ACCCCCAAGG TAAAGGATTT ACCCGTGGAG ACGACCACCA GGAGCCCCAC AAAAAAAACC 
ACGTCCTCCG GCACCGAGAA GTCCCTTAGC GAGGAGGCTT GGAATGATCT GATGGAGGCG 
GCCAAGCACA GCCAAGCTTT GGCTGATGAT CCACACTTCA ACAAGATGCT CAATACTACC 
GTCGCCGCCG GGGAGAAGAA CTCCACCGAT ACCGACGATA GCGAATACTA TGACTACGAT 
GATGATTACA ACTATTTCGA TGAGGAGACG GTGACGATTT CCCCCAGTCC CAAGAAGGCC 
AAGAGCAACC CCAAGCTGCA GCCCATTGTG GATGCCACCA TCACGAAGTC CAAGAGCAAA 
TCCAACGACC AAAAGACCAA AGAGAGTGCT AACGATATCG ACAGCGACTC TCAATATGAC 
TACGACGAGG GTCTGGATGA TGATGATGAC GAGGACGATG CCGCTGACGA CCTTTTGACC 
GATGATGAGC AAGTTGTCTT CAGCGAGGAC GTTCCGTGTC CAAGATTCTG CCAGTGTGCC 
CGGAATGTCA ACAGCTATCT GGTGGCCACC TGCAGCAGAC TTGACATGGG CATCCAGAAG 
TTTGGCTCTG ACATTACGGA TTTGGTTGTG ACCAATGTGG GTCCTAAGTA CCCCATCCTG 
ATGGGACCCA ACTTCTTCCA GAATCTGGGT CTTAAGAACG TGGCTTCCAT CAAGATCGCC 
AACTGCACCT TGGAGTACCT GCACGCCGAG GCCTTCCACG GACTGAACGA GCTGTACGCT 
GTTAACTTGA CGGATGTTGG TTTGGCTATC ATTAATCCCG ATACCTTTGT TGGAAACAAG 
AAACTGCGCA TGCTGACCAT TTCGGGTAAC GACTTGAGTG TGATGTCCAG CATTCACTAT 
TTGCTGAAGT CTTCCAGCAT TGAGGAGCTG GACTTCTCGC GCAACAACCT TATGGAGTTG 
AATCCCAAGG CCTTCTCCCA TCTATCCAAT GTCGTCTACA TTAACTTGAG CCAGAACAGT 
CTCAAGAAAC TGCCCGAGAA GGCTTTCGAG AAGGTTACTC TTCTGGAAGA ATTGGATCTG 
TCTTACAACT CGCTGACTGA ATTGCCCCGC GATATCTTCA ATGGCACCAC CCTGTCCATA 
CTGCACCTGA AGTACAATAC CTTCAATGGC GATCTGCACT TTGGCACCAA GGATCTGCAG 
CAGCTGGACT TGAGCTTTAA CTCGATTGTC CAAGTGCACC ACAGCATGTT CGACAAGATG 
CCCGGTCTGA CCAACCTGAA CCTCAAGGGC AATGGCATTA AGAAGATCCA ACCGGACTCG 
TTCCTTACTT TGAAGAACCT GCGCCACATC GATTTGTCCA TCAACGATCT GGACCAGATC 
TCGGGAATGC TGTTCTTTAA GAACTCTGAG CTGGATGTGA TCCGTTTGAA CGACAATCCT 
CGTCTGAGCC AGTTGCCCAC CGATGGCTTC TTGAGCTACA GTGGGGAGTT CACCGTCTAC 
TACCTGGATA TTTCCAATTG CGCCATCGGT CCATTGGGTC ACAAGGCCTT CTCCACTATG 
CCGCATTTGA CCACCTTGAA GTTGGCCTGG AACAACATTA ATCATCTGCC GCGCGAGATT 
TTCACTGGAC TGCACAAGCT GATTGACTTG GATCTGAGCA ACAACCTGAT CACCCGCATG 
GACGATCTGA TCTTCATGGA CAACGGAGAG CTGACTAAGC TCAGTCTGGC TGGAAATCCC 
ATTAGCCGCT TGTCCGTCCG CCTCTTCCTG CCGCTGCACC AACTGCGATG CCTGGACGTC 
AATGACTGCG AGCTTACCAC CTTGCTCTCC GATCGCGACT TGGGTGCGGG TTACAAGATC 
TTTGACAGCC TGCGCTCCTT TAACGCCTCT GGTAATTTGA TCAAGAAGAT CTCTTCGGAG 
GATGTGAAGA GCTTCAAGAA CCTGCGTTCT CTGGATATCA CCAACAATCC GCTGAAGTGC 
ACTCCCGACT TCCAGGAGTT CATCAGCTAC GTCACTCTTC AGATGCAGAT GACTCCCAAG 
CGTCTGCCCG TGCTGGCCAA TCTGGAGGAC GATGCTACTA TTGTGCAGCT GGAGACTCTG 
GCTCAGGCTG GTTGGTCGTC TTTGGCCCAC GAGGTGTGCA AGCATGCCGA AGGATCAGAT 
CTCTTGGACG AAAAGAAGGC CGACAGTGCC GAAGCCAAAC TGGAGAAGCG TCTGAAGGAA 
AGCGTGAAGA AGCTTAACGA GGATGAGGCT AAGTTGCTGA GTGTCCTGGA CAAGAGTGTG 
CTAGACAAGA GCAGGCTGAG CAGCATGCAG AAGATCATGA AGGGATCGGT GAACACTGAA 
ACCGCGAAGC CTGAGGAAGA TGCTGACGAG GAGAATCTGA ACAATGGCGG CGAGGATAAG 
GAGGATGACA GCAGTGACTA TGACAGTGAG GAGGATGATG ATGACGATGA TGAGGACGAT 
GATGATGACA ACAACGATGA TGATCGCTTC GAGGAGGCCA AGAAGAATGC CAAGGCTAAG 
GCCGATGCTA ACAAGGCCTT CGACACGTTC GTGAACAAGG AACTGAAGCC AGTCCTGAAG 
ACCGATGGTA TCCAGGCGGA GGAAGTGGAC ATGTCTCAGG AGACCCGTGA TAAATTCCTT 
CTGGAGAAAC TGGGCTTCGA CAGCGAAAGC GAGGAGTCAG ATGAGTACAC CGAGAAGATG 
ATCTTCCACG GCGAACTATC CTACGACCTG GTGGTCCCAC TGATCGTCGT GTCCTTCTGC 
CTCCTGATGA TCGTGCTGTC CATCGCCCGA GTGATCTACG TGGTGCTGCG CAAGCGCGGC 
GAGCGCTATC GCATGGCGCT GCTGGCCTCA AAGAACTCGT TCGTATACCA GAAGTTGAGC 
GAGGACATCG TGAAGCCGAC CAGCAAGGAG CAGAAGGAGC CCAAGCATCT GAAGCAGCCA 
AAGGTGCACC GATACGCGCC CATCAATCAG GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gp150 ( NM_166528.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726191.1 Drosophila melanogaster Gp150, transcript variant B (Gp150), mRNA.

Price & Availability↑

CloneID	OFa10912
Clone ID Related Accession (Same CDS sequence)	NM_001299785.1 , NM_166528.2 , NM_166529.2 , NM_166527.2
Accession Version	NM_166528.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Gp150, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166528.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTCGCC AGTCGACTTG CAGGCTAGTC CTGCTGCTGG CCTTTACCCT GGCCCTAATC 
CACGCCAGCC ACTCAAATAC CGTGGGCCCC AAGATCCACT TCATTCATGA CGATCTGACG 
GAAAACGAGC TGAACTCTGG AGAGGAGGTG AGCCCGACCG AGAGTCTGCC CAAAATCTCC 
ACCCCCAAGG TAAAGGATTT ACCCGTGGAG ACGACCACCA GGAGCCCCAC AAAAAAAACC 
ACGTCCTCCG GCACCGAGAA GTCCCTTAGC GAGGAGGCTT GGAATGATCT GATGGAGGCG 
GCCAAGCACA GCCAAGCTTT GGCTGATGAT CCACACTTCA ACAAGATGCT CAATACTACC 
GTCGCCGCCG GGGAGAAGAA CTCCACCGAT ACCGACGATA GCGAATACTA TGACTACGAT 
GATGATTACA ACTATTTCGA TGAGGAGACG GTGACGATTT CCCCCAGTCC CAAGAAGGCC 
AAGAGCAACC CCAAGCTGCA GCCCATTGTG GATGCCACCA TCACGAAGTC CAAGAGCAAA 
TCCAACGACC AAAAGACCAA AGAGAGTGCT AACGATATCG ACAGCGACTC TCAATATGAC 
TACGACGAGG GTCTGGATGA TGATGATGAC GAGGACGATG CCGCTGACGA CCTTTTGACC 
GATGATGAGC AAGTTGTCTT CAGCGAGGAC GTTCCGTGTC CAAGATTCTG CCAGTGTGCC 
CGGAATGTCA ACAGCTATCT GGTGGCCACC TGCAGCAGAC TTGACATGGG CATCCAGAAG 
TTTGGCTCTG ACATTACGGA TTTGGTTGTG ACCAATGTGG GTCCTAAGTA CCCCATCCTG 
ATGGGACCCA ACTTCTTCCA GAATCTGGGT CTTAAGAACG TGGCTTCCAT CAAGATCGCC 
AACTGCACCT TGGAGTACCT GCACGCCGAG GCCTTCCACG GACTGAACGA GCTGTACGCT 
GTTAACTTGA CGGATGTTGG TTTGGCTATC ATTAATCCCG ATACCTTTGT TGGAAACAAG 
AAACTGCGCA TGCTGACCAT TTCGGGTAAC GACTTGAGTG TGATGTCCAG CATTCACTAT 
TTGCTGAAGT CTTCCAGCAT TGAGGAGCTG GACTTCTCGC GCAACAACCT TATGGAGTTG 
AATCCCAAGG CCTTCTCCCA TCTATCCAAT GTCGTCTACA TTAACTTGAG CCAGAACAGT 
CTCAAGAAAC TGCCCGAGAA GGCTTTCGAG AAGGTTACTC TTCTGGAAGA ATTGGATCTG 
TCTTACAACT CGCTGACTGA ATTGCCCCGC GATATCTTCA ATGGCACCAC CCTGTCCATA 
CTGCACCTGA AGTACAATAC CTTCAATGGC GATCTGCACT TTGGCACCAA GGATCTGCAG 
CAGCTGGACT TGAGCTTTAA CTCGATTGTC CAAGTGCACC ACAGCATGTT CGACAAGATG 
CCCGGTCTGA CCAACCTGAA CCTCAAGGGC AATGGCATTA AGAAGATCCA ACCGGACTCG 
TTCCTTACTT TGAAGAACCT GCGCCACATC GATTTGTCCA TCAACGATCT GGACCAGATC 
TCGGGAATGC TGTTCTTTAA GAACTCTGAG CTGGATGTGA TCCGTTTGAA CGACAATCCT 
CGTCTGAGCC AGTTGCCCAC CGATGGCTTC TTGAGCTACA GTGGGGAGTT CACCGTCTAC 
TACCTGGATA TTTCCAATTG CGCCATCGGT CCATTGGGTC ACAAGGCCTT CTCCACTATG 
CCGCATTTGA CCACCTTGAA GTTGGCCTGG AACAACATTA ATCATCTGCC GCGCGAGATT 
TTCACTGGAC TGCACAAGCT GATTGACTTG GATCTGAGCA ACAACCTGAT CACCCGCATG 
GACGATCTGA TCTTCATGGA CAACGGAGAG CTGACTAAGC TCAGTCTGGC TGGAAATCCC 
ATTAGCCGCT TGTCCGTCCG CCTCTTCCTG CCGCTGCACC AACTGCGATG CCTGGACGTC 
AATGACTGCG AGCTTACCAC CTTGCTCTCC GATCGCGACT TGGGTGCGGG TTACAAGATC 
TTTGACAGCC TGCGCTCCTT TAACGCCTCT GGTAATTTGA TCAAGAAGAT CTCTTCGGAG 
GATGTGAAGA GCTTCAAGAA CCTGCGTTCT CTGGATATCA CCAACAATCC GCTGAAGTGC 
ACTCCCGACT TCCAGGAGTT CATCAGCTAC GTCACTCTTC AGATGCAGAT GACTCCCAAG 
CGTCTGCCCG TGCTGGCCAA TCTGGAGGAC GATGCTACTA TTGTGCAGCT GGAGACTCTG 
GCTCAGGCTG GTTGGTCGTC TTTGGCCCAC GAGGTGTGCA AGCATGCCGA AGGATCAGAT 
CTCTTGGACG AAAAGAAGGC CGACAGTGCC GAAGCCAAAC TGGAGAAGCG TCTGAAGGAA 
AGCGTGAAGA AGCTTAACGA GGATGAGGCT AAGTTGCTGA GTGTCCTGGA CAAGAGTGTG 
CTAGACAAGA GCAGGCTGAG CAGCATGCAG AAGATCATGA AGGGATCGGT GAACACTGAA 
ACCGCGAAGC CTGAGGAAGA TGCTGACGAG GAGAATCTGA ACAATGGCGG CGAGGATAAG 
GAGGATGACA GCAGTGACTA TGACAGTGAG GAGGATGATG ATGACGATGA TGAGGACGAT 
GATGATGACA ACAACGATGA TGATCGCTTC GAGGAGGCCA AGAAGAATGC CAAGGCTAAG 
GCCGATGCTA ACAAGGCCTT CGACACGTTC GTGAACAAGG AACTGAAGCC AGTCCTGAAG 
ACCGATGGTA TCCAGGCGGA GGAAGTGGAC ATGTCTCAGG AGACCCGTGA TAAATTCCTT 
CTGGAGAAAC TGGGCTTCGA CAGCGAAAGC GAGGAGTCAG ATGAGTACAC CGAGAAGATG 
ATCTTCCACG GCGAACTATC CTACGACCTG GTGGTCCCAC TGATCGTCGT GTCCTTCTGC 
CTCCTGATGA TCGTGCTGTC CATCGCCCGA GTGATCTACG TGGTGCTGCG CAAGCGCGGC 
GAGCGCTATC GCATGGCGCT GCTGGCCTCA AAGAACTCGT TCGTATACCA GAAGTTGAGC 
GAGGACATCG TGAAGCCGAC CAGCAAGGAG CAGAAGGAGC CCAAGCATCT GAAGCAGCCA 
AAGGTGCACC GATACGCGCC CATCAATCAG GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726191.1
CDS	208..3363
Translation	MSRQSTCRLVLLLAFTLALIHASHSNTVGPKIHFIHDDLTENELNSGEEVSPTESLPKISTPKVKDLPVETTTRSPTKKTTSSGTEKSLSEEAWNDLMEAAKHSQALADDPHFNKMLNTTVAAGEKNSTDTDDSEYYDYDDDYNYFDEETVTISPSPKKAKSNPKLQPIVDATITKSKSKSNDQKTKESANDIDSDSQYDYDEGLDDDDDEDDAADDLLTDDEQVVFSEDVPCPRFCQCARNVNSYLVATCSRLDMGIQKFGSDITDLVVTNVGPKYPILMGPNFFQNLGLKNVASIKIANCTLEYLHAEAFHGLNELYAVNLTDVGLAIINPDTFVGNKKLRMLTISGNDLSVMSSIHYLLKSSSIEELDFSRNNLMELNPKAFSHLSNVVYINLSQNSLKKLPEKAFEKVTLLEELDLSYNSLTELPRDIFNGTTLSILHLKYNTFNGDLHFGTKDLQQLDLSFNSIVQVHHSMFDKMPGLTNLNLKGNGIKKIQPDSFLTLKNLRHIDLSINDLDQISGMLFFKNSELDVIRLNDNPRLSQLPTDGFLSYSGEFTVYYLDISNCAIGPLGHKAFSTMPHLTTLKLAWNNINHLPREIFTGLHKLIDLDLSNNLITRMDDLIFMDNGELTKLSLAGNPISRLSVRLFLPLHQLRCLDVNDCELTTLLSDRDLGAGYKIFDSLRSFNASGNLIKKISSEDVKSFKNLRSLDITNNPLKCTPDFQEFISYVTLQMQMTPKRLPVLANLEDDATIVQLETLAQAGWSSLAHEVCKHAEGSDLLDEKKADSAEAKLEKRLKESVKKLNEDEAKLLSVLDKSVLDKSRLSSMQKIMKGSVNTETAKPEEDADEENLNNGGEDKEDDSSDYDSEEDDDDDDEDDDDDNNDDDRFEEAKKNAKAKADANKAFDTFVNKELKPVLKTDGIQAEEVDMSQETRDKFLLEKLGFDSESEESDEYTEKMIFHGELSYDLVVPLIVVSFCLLMIVLSIARVIYVVLRKRGERYRMALLASKNSFVYQKLSEDIVKPTSKEQKEPKHLKQPKVHRYAPINQV

Target ORF information:

RefSeq Version	NM_166528.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Gp150, transcript variant B (Gp150), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166528.2

ORF Insert Sequence:

ATGAGTCGCC AGTCGACTTG CAGGCTAGTC CTGCTGCTGG CCTTTACCCT GGCCCTAATC 
CACGCCAGCC ACTCAAATAC CGTGGGCCCC AAGATCCACT TCATTCATGA CGATCTGACG 
GAAAACGAGC TGAACTCTGG AGAGGAGGTG AGCCCGACCG AGAGTCTGCC CAAAATCTCC 
ACCCCCAAGG TAAAGGATTT ACCCGTGGAG ACGACCACCA GGAGCCCCAC AAAAAAAACC 
ACGTCCTCCG GCACCGAGAA GTCCCTTAGC GAGGAGGCTT GGAATGATCT GATGGAGGCG 
GCCAAGCACA GCCAAGCTTT GGCTGATGAT CCACACTTCA ACAAGATGCT CAATACTACC 
GTCGCCGCCG GGGAGAAGAA CTCCACCGAT ACCGACGATA GCGAATACTA TGACTACGAT 
GATGATTACA ACTATTTCGA TGAGGAGACG GTGACGATTT CCCCCAGTCC CAAGAAGGCC 
AAGAGCAACC CCAAGCTGCA GCCCATTGTG GATGCCACCA TCACGAAGTC CAAGAGCAAA 
TCCAACGACC AAAAGACCAA AGAGAGTGCT AACGATATCG ACAGCGACTC TCAATATGAC 
TACGACGAGG GTCTGGATGA TGATGATGAC GAGGACGATG CCGCTGACGA CCTTTTGACC 
GATGATGAGC AAGTTGTCTT CAGCGAGGAC GTTCCGTGTC CAAGATTCTG CCAGTGTGCC 
CGGAATGTCA ACAGCTATCT GGTGGCCACC TGCAGCAGAC TTGACATGGG CATCCAGAAG 
TTTGGCTCTG ACATTACGGA TTTGGTTGTG ACCAATGTGG GTCCTAAGTA CCCCATCCTG 
ATGGGACCCA ACTTCTTCCA GAATCTGGGT CTTAAGAACG TGGCTTCCAT CAAGATCGCC 
AACTGCACCT TGGAGTACCT GCACGCCGAG GCCTTCCACG GACTGAACGA GCTGTACGCT 
GTTAACTTGA CGGATGTTGG TTTGGCTATC ATTAATCCCG ATACCTTTGT TGGAAACAAG 
AAACTGCGCA TGCTGACCAT TTCGGGTAAC GACTTGAGTG TGATGTCCAG CATTCACTAT 
TTGCTGAAGT CTTCCAGCAT TGAGGAGCTG GACTTCTCGC GCAACAACCT TATGGAGTTG 
AATCCCAAGG CCTTCTCCCA TCTATCCAAT GTCGTCTACA TTAACTTGAG CCAGAACAGT 
CTCAAGAAAC TGCCCGAGAA GGCTTTCGAG AAGGTTACTC TTCTGGAAGA ATTGGATCTG 
TCTTACAACT CGCTGACTGA ATTGCCCCGC GATATCTTCA ATGGCACCAC CCTGTCCATA 
CTGCACCTGA AGTACAATAC CTTCAATGGC GATCTGCACT TTGGCACCAA GGATCTGCAG 
CAGCTGGACT TGAGCTTTAA CTCGATTGTC CAAGTGCACC ACAGCATGTT CGACAAGATG 
CCCGGTCTGA CCAACCTGAA CCTCAAGGGC AATGGCATTA AGAAGATCCA ACCGGACTCG 
TTCCTTACTT TGAAGAACCT GCGCCACATC GATTTGTCCA TCAACGATCT GGACCAGATC 
TCGGGAATGC TGTTCTTTAA GAACTCTGAG CTGGATGTGA TCCGTTTGAA CGACAATCCT 
CGTCTGAGCC AGTTGCCCAC CGATGGCTTC TTGAGCTACA GTGGGGAGTT CACCGTCTAC 
TACCTGGATA TTTCCAATTG CGCCATCGGT CCATTGGGTC ACAAGGCCTT CTCCACTATG 
CCGCATTTGA CCACCTTGAA GTTGGCCTGG AACAACATTA ATCATCTGCC GCGCGAGATT 
TTCACTGGAC TGCACAAGCT GATTGACTTG GATCTGAGCA ACAACCTGAT CACCCGCATG 
GACGATCTGA TCTTCATGGA CAACGGAGAG CTGACTAAGC TCAGTCTGGC TGGAAATCCC 
ATTAGCCGCT TGTCCGTCCG CCTCTTCCTG CCGCTGCACC AACTGCGATG CCTGGACGTC 
AATGACTGCG AGCTTACCAC CTTGCTCTCC GATCGCGACT TGGGTGCGGG TTACAAGATC 
TTTGACAGCC TGCGCTCCTT TAACGCCTCT GGTAATTTGA TCAAGAAGAT CTCTTCGGAG 
GATGTGAAGA GCTTCAAGAA CCTGCGTTCT CTGGATATCA CCAACAATCC GCTGAAGTGC 
ACTCCCGACT TCCAGGAGTT CATCAGCTAC GTCACTCTTC AGATGCAGAT GACTCCCAAG 
CGTCTGCCCG TGCTGGCCAA TCTGGAGGAC GATGCTACTA TTGTGCAGCT GGAGACTCTG 
GCTCAGGCTG GTTGGTCGTC TTTGGCCCAC GAGGTGTGCA AGCATGCCGA AGGATCAGAT 
CTCTTGGACG AAAAGAAGGC CGACAGTGCC GAAGCCAAAC TGGAGAAGCG TCTGAAGGAA 
AGCGTGAAGA AGCTTAACGA GGATGAGGCT AAGTTGCTGA GTGTCCTGGA CAAGAGTGTG 
CTAGACAAGA GCAGGCTGAG CAGCATGCAG AAGATCATGA AGGGATCGGT GAACACTGAA 
ACCGCGAAGC CTGAGGAAGA TGCTGACGAG GAGAATCTGA ACAATGGCGG CGAGGATAAG 
GAGGATGACA GCAGTGACTA TGACAGTGAG GAGGATGATG ATGACGATGA TGAGGACGAT 
GATGATGACA ACAACGATGA TGATCGCTTC GAGGAGGCCA AGAAGAATGC CAAGGCTAAG 
GCCGATGCTA ACAAGGCCTT CGACACGTTC GTGAACAAGG AACTGAAGCC AGTCCTGAAG 
ACCGATGGTA TCCAGGCGGA GGAAGTGGAC ATGTCTCAGG AGACCCGTGA TAAATTCCTT 
CTGGAGAAAC TGGGCTTCGA CAGCGAAAGC GAGGAGTCAG ATGAGTACAC CGAGAAGATG 
ATCTTCCACG GCGAACTATC CTACGACCTG GTGGTCCCAC TGATCGTCGT GTCCTTCTGC 
CTCCTGATGA TCGTGCTGTC CATCGCCCGA GTGATCTACG TGGTGCTGCG CAAGCGCGGC 
GAGCGCTATC GCATGGCGCT GCTGGCCTCA AAGAACTCGT TCGTATACCA GAAGTTGAGC 
GAGGACATCG TGAAGCCGAC CAGCAAGGAG CAGAAGGAGC CCAAGCATCT GAAGCAGCCA 
AAGGTGCACC GATACGCGCC CATCAATCAG GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gp150 ( NM_166529.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726192.1 Drosophila melanogaster Gp150, transcript variant C (Gp150), mRNA.

Price & Availability↑

CloneID	OFa10912
Clone ID Related Accession (Same CDS sequence)	NM_001299785.1 , NM_166528.2 , NM_166529.2 , NM_166527.2
Accession Version	NM_166529.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Gp150, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166529.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTCGCC AGTCGACTTG CAGGCTAGTC CTGCTGCTGG CCTTTACCCT GGCCCTAATC 
CACGCCAGCC ACTCAAATAC CGTGGGCCCC AAGATCCACT TCATTCATGA CGATCTGACG 
GAAAACGAGC TGAACTCTGG AGAGGAGGTG AGCCCGACCG AGAGTCTGCC CAAAATCTCC 
ACCCCCAAGG TAAAGGATTT ACCCGTGGAG ACGACCACCA GGAGCCCCAC AAAAAAAACC 
ACGTCCTCCG GCACCGAGAA GTCCCTTAGC GAGGAGGCTT GGAATGATCT GATGGAGGCG 
GCCAAGCACA GCCAAGCTTT GGCTGATGAT CCACACTTCA ACAAGATGCT CAATACTACC 
GTCGCCGCCG GGGAGAAGAA CTCCACCGAT ACCGACGATA GCGAATACTA TGACTACGAT 
GATGATTACA ACTATTTCGA TGAGGAGACG GTGACGATTT CCCCCAGTCC CAAGAAGGCC 
AAGAGCAACC CCAAGCTGCA GCCCATTGTG GATGCCACCA TCACGAAGTC CAAGAGCAAA 
TCCAACGACC AAAAGACCAA AGAGAGTGCT AACGATATCG ACAGCGACTC TCAATATGAC 
TACGACGAGG GTCTGGATGA TGATGATGAC GAGGACGATG CCGCTGACGA CCTTTTGACC 
GATGATGAGC AAGTTGTCTT CAGCGAGGAC GTTCCGTGTC CAAGATTCTG CCAGTGTGCC 
CGGAATGTCA ACAGCTATCT GGTGGCCACC TGCAGCAGAC TTGACATGGG CATCCAGAAG 
TTTGGCTCTG ACATTACGGA TTTGGTTGTG ACCAATGTGG GTCCTAAGTA CCCCATCCTG 
ATGGGACCCA ACTTCTTCCA GAATCTGGGT CTTAAGAACG TGGCTTCCAT CAAGATCGCC 
AACTGCACCT TGGAGTACCT GCACGCCGAG GCCTTCCACG GACTGAACGA GCTGTACGCT 
GTTAACTTGA CGGATGTTGG TTTGGCTATC ATTAATCCCG ATACCTTTGT TGGAAACAAG 
AAACTGCGCA TGCTGACCAT TTCGGGTAAC GACTTGAGTG TGATGTCCAG CATTCACTAT 
TTGCTGAAGT CTTCCAGCAT TGAGGAGCTG GACTTCTCGC GCAACAACCT TATGGAGTTG 
AATCCCAAGG CCTTCTCCCA TCTATCCAAT GTCGTCTACA TTAACTTGAG CCAGAACAGT 
CTCAAGAAAC TGCCCGAGAA GGCTTTCGAG AAGGTTACTC TTCTGGAAGA ATTGGATCTG 
TCTTACAACT CGCTGACTGA ATTGCCCCGC GATATCTTCA ATGGCACCAC CCTGTCCATA 
CTGCACCTGA AGTACAATAC CTTCAATGGC GATCTGCACT TTGGCACCAA GGATCTGCAG 
CAGCTGGACT TGAGCTTTAA CTCGATTGTC CAAGTGCACC ACAGCATGTT CGACAAGATG 
CCCGGTCTGA CCAACCTGAA CCTCAAGGGC AATGGCATTA AGAAGATCCA ACCGGACTCG 
TTCCTTACTT TGAAGAACCT GCGCCACATC GATTTGTCCA TCAACGATCT GGACCAGATC 
TCGGGAATGC TGTTCTTTAA GAACTCTGAG CTGGATGTGA TCCGTTTGAA CGACAATCCT 
CGTCTGAGCC AGTTGCCCAC CGATGGCTTC TTGAGCTACA GTGGGGAGTT CACCGTCTAC 
TACCTGGATA TTTCCAATTG CGCCATCGGT CCATTGGGTC ACAAGGCCTT CTCCACTATG 
CCGCATTTGA CCACCTTGAA GTTGGCCTGG AACAACATTA ATCATCTGCC GCGCGAGATT 
TTCACTGGAC TGCACAAGCT GATTGACTTG GATCTGAGCA ACAACCTGAT CACCCGCATG 
GACGATCTGA TCTTCATGGA CAACGGAGAG CTGACTAAGC TCAGTCTGGC TGGAAATCCC 
ATTAGCCGCT TGTCCGTCCG CCTCTTCCTG CCGCTGCACC AACTGCGATG CCTGGACGTC 
AATGACTGCG AGCTTACCAC CTTGCTCTCC GATCGCGACT TGGGTGCGGG TTACAAGATC 
TTTGACAGCC TGCGCTCCTT TAACGCCTCT GGTAATTTGA TCAAGAAGAT CTCTTCGGAG 
GATGTGAAGA GCTTCAAGAA CCTGCGTTCT CTGGATATCA CCAACAATCC GCTGAAGTGC 
ACTCCCGACT TCCAGGAGTT CATCAGCTAC GTCACTCTTC AGATGCAGAT GACTCCCAAG 
CGTCTGCCCG TGCTGGCCAA TCTGGAGGAC GATGCTACTA TTGTGCAGCT GGAGACTCTG 
GCTCAGGCTG GTTGGTCGTC TTTGGCCCAC GAGGTGTGCA AGCATGCCGA AGGATCAGAT 
CTCTTGGACG AAAAGAAGGC CGACAGTGCC GAAGCCAAAC TGGAGAAGCG TCTGAAGGAA 
AGCGTGAAGA AGCTTAACGA GGATGAGGCT AAGTTGCTGA GTGTCCTGGA CAAGAGTGTG 
CTAGACAAGA GCAGGCTGAG CAGCATGCAG AAGATCATGA AGGGATCGGT GAACACTGAA 
ACCGCGAAGC CTGAGGAAGA TGCTGACGAG GAGAATCTGA ACAATGGCGG CGAGGATAAG 
GAGGATGACA GCAGTGACTA TGACAGTGAG GAGGATGATG ATGACGATGA TGAGGACGAT 
GATGATGACA ACAACGATGA TGATCGCTTC GAGGAGGCCA AGAAGAATGC CAAGGCTAAG 
GCCGATGCTA ACAAGGCCTT CGACACGTTC GTGAACAAGG AACTGAAGCC AGTCCTGAAG 
ACCGATGGTA TCCAGGCGGA GGAAGTGGAC ATGTCTCAGG AGACCCGTGA TAAATTCCTT 
CTGGAGAAAC TGGGCTTCGA CAGCGAAAGC GAGGAGTCAG ATGAGTACAC CGAGAAGATG 
ATCTTCCACG GCGAACTATC CTACGACCTG GTGGTCCCAC TGATCGTCGT GTCCTTCTGC 
CTCCTGATGA TCGTGCTGTC CATCGCCCGA GTGATCTACG TGGTGCTGCG CAAGCGCGGC 
GAGCGCTATC GCATGGCGCT GCTGGCCTCA AAGAACTCGT TCGTATACCA GAAGTTGAGC 
GAGGACATCG TGAAGCCGAC CAGCAAGGAG CAGAAGGAGC CCAAGCATCT GAAGCAGCCA 
AAGGTGCACC GATACGCGCC CATCAATCAG GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726192.1
CDS	313..3468
Translation	MSRQSTCRLVLLLAFTLALIHASHSNTVGPKIHFIHDDLTENELNSGEEVSPTESLPKISTPKVKDLPVETTTRSPTKKTTSSGTEKSLSEEAWNDLMEAAKHSQALADDPHFNKMLNTTVAAGEKNSTDTDDSEYYDYDDDYNYFDEETVTISPSPKKAKSNPKLQPIVDATITKSKSKSNDQKTKESANDIDSDSQYDYDEGLDDDDDEDDAADDLLTDDEQVVFSEDVPCPRFCQCARNVNSYLVATCSRLDMGIQKFGSDITDLVVTNVGPKYPILMGPNFFQNLGLKNVASIKIANCTLEYLHAEAFHGLNELYAVNLTDVGLAIINPDTFVGNKKLRMLTISGNDLSVMSSIHYLLKSSSIEELDFSRNNLMELNPKAFSHLSNVVYINLSQNSLKKLPEKAFEKVTLLEELDLSYNSLTELPRDIFNGTTLSILHLKYNTFNGDLHFGTKDLQQLDLSFNSIVQVHHSMFDKMPGLTNLNLKGNGIKKIQPDSFLTLKNLRHIDLSINDLDQISGMLFFKNSELDVIRLNDNPRLSQLPTDGFLSYSGEFTVYYLDISNCAIGPLGHKAFSTMPHLTTLKLAWNNINHLPREIFTGLHKLIDLDLSNNLITRMDDLIFMDNGELTKLSLAGNPISRLSVRLFLPLHQLRCLDVNDCELTTLLSDRDLGAGYKIFDSLRSFNASGNLIKKISSEDVKSFKNLRSLDITNNPLKCTPDFQEFISYVTLQMQMTPKRLPVLANLEDDATIVQLETLAQAGWSSLAHEVCKHAEGSDLLDEKKADSAEAKLEKRLKESVKKLNEDEAKLLSVLDKSVLDKSRLSSMQKIMKGSVNTETAKPEEDADEENLNNGGEDKEDDSSDYDSEEDDDDDDEDDDDDNNDDDRFEEAKKNAKAKADANKAFDTFVNKELKPVLKTDGIQAEEVDMSQETRDKFLLEKLGFDSESEESDEYTEKMIFHGELSYDLVVPLIVVSFCLLMIVLSIARVIYVVLRKRGERYRMALLASKNSFVYQKLSEDIVKPTSKEQKEPKHLKQPKVHRYAPINQV

Target ORF information:

RefSeq Version	NM_166529.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Gp150, transcript variant C (Gp150), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166529.2

ORF Insert Sequence:

ATGAGTCGCC AGTCGACTTG CAGGCTAGTC CTGCTGCTGG CCTTTACCCT GGCCCTAATC 
CACGCCAGCC ACTCAAATAC CGTGGGCCCC AAGATCCACT TCATTCATGA CGATCTGACG 
GAAAACGAGC TGAACTCTGG AGAGGAGGTG AGCCCGACCG AGAGTCTGCC CAAAATCTCC 
ACCCCCAAGG TAAAGGATTT ACCCGTGGAG ACGACCACCA GGAGCCCCAC AAAAAAAACC 
ACGTCCTCCG GCACCGAGAA GTCCCTTAGC GAGGAGGCTT GGAATGATCT GATGGAGGCG 
GCCAAGCACA GCCAAGCTTT GGCTGATGAT CCACACTTCA ACAAGATGCT CAATACTACC 
GTCGCCGCCG GGGAGAAGAA CTCCACCGAT ACCGACGATA GCGAATACTA TGACTACGAT 
GATGATTACA ACTATTTCGA TGAGGAGACG GTGACGATTT CCCCCAGTCC CAAGAAGGCC 
AAGAGCAACC CCAAGCTGCA GCCCATTGTG GATGCCACCA TCACGAAGTC CAAGAGCAAA 
TCCAACGACC AAAAGACCAA AGAGAGTGCT AACGATATCG ACAGCGACTC TCAATATGAC 
TACGACGAGG GTCTGGATGA TGATGATGAC GAGGACGATG CCGCTGACGA CCTTTTGACC 
GATGATGAGC AAGTTGTCTT CAGCGAGGAC GTTCCGTGTC CAAGATTCTG CCAGTGTGCC 
CGGAATGTCA ACAGCTATCT GGTGGCCACC TGCAGCAGAC TTGACATGGG CATCCAGAAG 
TTTGGCTCTG ACATTACGGA TTTGGTTGTG ACCAATGTGG GTCCTAAGTA CCCCATCCTG 
ATGGGACCCA ACTTCTTCCA GAATCTGGGT CTTAAGAACG TGGCTTCCAT CAAGATCGCC 
AACTGCACCT TGGAGTACCT GCACGCCGAG GCCTTCCACG GACTGAACGA GCTGTACGCT 
GTTAACTTGA CGGATGTTGG TTTGGCTATC ATTAATCCCG ATACCTTTGT TGGAAACAAG 
AAACTGCGCA TGCTGACCAT TTCGGGTAAC GACTTGAGTG TGATGTCCAG CATTCACTAT 
TTGCTGAAGT CTTCCAGCAT TGAGGAGCTG GACTTCTCGC GCAACAACCT TATGGAGTTG 
AATCCCAAGG CCTTCTCCCA TCTATCCAAT GTCGTCTACA TTAACTTGAG CCAGAACAGT 
CTCAAGAAAC TGCCCGAGAA GGCTTTCGAG AAGGTTACTC TTCTGGAAGA ATTGGATCTG 
TCTTACAACT CGCTGACTGA ATTGCCCCGC GATATCTTCA ATGGCACCAC CCTGTCCATA 
CTGCACCTGA AGTACAATAC CTTCAATGGC GATCTGCACT TTGGCACCAA GGATCTGCAG 
CAGCTGGACT TGAGCTTTAA CTCGATTGTC CAAGTGCACC ACAGCATGTT CGACAAGATG 
CCCGGTCTGA CCAACCTGAA CCTCAAGGGC AATGGCATTA AGAAGATCCA ACCGGACTCG 
TTCCTTACTT TGAAGAACCT GCGCCACATC GATTTGTCCA TCAACGATCT GGACCAGATC 
TCGGGAATGC TGTTCTTTAA GAACTCTGAG CTGGATGTGA TCCGTTTGAA CGACAATCCT 
CGTCTGAGCC AGTTGCCCAC CGATGGCTTC TTGAGCTACA GTGGGGAGTT CACCGTCTAC 
TACCTGGATA TTTCCAATTG CGCCATCGGT CCATTGGGTC ACAAGGCCTT CTCCACTATG 
CCGCATTTGA CCACCTTGAA GTTGGCCTGG AACAACATTA ATCATCTGCC GCGCGAGATT 
TTCACTGGAC TGCACAAGCT GATTGACTTG GATCTGAGCA ACAACCTGAT CACCCGCATG 
GACGATCTGA TCTTCATGGA CAACGGAGAG CTGACTAAGC TCAGTCTGGC TGGAAATCCC 
ATTAGCCGCT TGTCCGTCCG CCTCTTCCTG CCGCTGCACC AACTGCGATG CCTGGACGTC 
AATGACTGCG AGCTTACCAC CTTGCTCTCC GATCGCGACT TGGGTGCGGG TTACAAGATC 
TTTGACAGCC TGCGCTCCTT TAACGCCTCT GGTAATTTGA TCAAGAAGAT CTCTTCGGAG 
GATGTGAAGA GCTTCAAGAA CCTGCGTTCT CTGGATATCA CCAACAATCC GCTGAAGTGC 
ACTCCCGACT TCCAGGAGTT CATCAGCTAC GTCACTCTTC AGATGCAGAT GACTCCCAAG 
CGTCTGCCCG TGCTGGCCAA TCTGGAGGAC GATGCTACTA TTGTGCAGCT GGAGACTCTG 
GCTCAGGCTG GTTGGTCGTC TTTGGCCCAC GAGGTGTGCA AGCATGCCGA AGGATCAGAT 
CTCTTGGACG AAAAGAAGGC CGACAGTGCC GAAGCCAAAC TGGAGAAGCG TCTGAAGGAA 
AGCGTGAAGA AGCTTAACGA GGATGAGGCT AAGTTGCTGA GTGTCCTGGA CAAGAGTGTG 
CTAGACAAGA GCAGGCTGAG CAGCATGCAG AAGATCATGA AGGGATCGGT GAACACTGAA 
ACCGCGAAGC CTGAGGAAGA TGCTGACGAG GAGAATCTGA ACAATGGCGG CGAGGATAAG 
GAGGATGACA GCAGTGACTA TGACAGTGAG GAGGATGATG ATGACGATGA TGAGGACGAT 
GATGATGACA ACAACGATGA TGATCGCTTC GAGGAGGCCA AGAAGAATGC CAAGGCTAAG 
GCCGATGCTA ACAAGGCCTT CGACACGTTC GTGAACAAGG AACTGAAGCC AGTCCTGAAG 
ACCGATGGTA TCCAGGCGGA GGAAGTGGAC ATGTCTCAGG AGACCCGTGA TAAATTCCTT 
CTGGAGAAAC TGGGCTTCGA CAGCGAAAGC GAGGAGTCAG ATGAGTACAC CGAGAAGATG 
ATCTTCCACG GCGAACTATC CTACGACCTG GTGGTCCCAC TGATCGTCGT GTCCTTCTGC 
CTCCTGATGA TCGTGCTGTC CATCGCCCGA GTGATCTACG TGGTGCTGCG CAAGCGCGGC 
GAGCGCTATC GCATGGCGCT GCTGGCCTCA AAGAACTCGT TCGTATACCA GAAGTTGAGC 
GAGGACATCG TGAAGCCGAC CAGCAAGGAG CAGAAGGAGC CCAAGCATCT GAAGCAGCCA 
AAGGTGCACC GATACGCGCC CATCAATCAG GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gp150 ( NM_166527.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726190.1 Drosophila melanogaster Gp150, transcript variant A (Gp150), mRNA.

Price & Availability↑

CloneID	OFa10912
Clone ID Related Accession (Same CDS sequence)	NM_001299785.1 , NM_166528.2 , NM_166529.2 , NM_166527.2
Accession Version	NM_166527.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Gp150, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166527.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTCGCC AGTCGACTTG CAGGCTAGTC CTGCTGCTGG CCTTTACCCT GGCCCTAATC 
CACGCCAGCC ACTCAAATAC CGTGGGCCCC AAGATCCACT TCATTCATGA CGATCTGACG 
GAAAACGAGC TGAACTCTGG AGAGGAGGTG AGCCCGACCG AGAGTCTGCC CAAAATCTCC 
ACCCCCAAGG TAAAGGATTT ACCCGTGGAG ACGACCACCA GGAGCCCCAC AAAAAAAACC 
ACGTCCTCCG GCACCGAGAA GTCCCTTAGC GAGGAGGCTT GGAATGATCT GATGGAGGCG 
GCCAAGCACA GCCAAGCTTT GGCTGATGAT CCACACTTCA ACAAGATGCT CAATACTACC 
GTCGCCGCCG GGGAGAAGAA CTCCACCGAT ACCGACGATA GCGAATACTA TGACTACGAT 
GATGATTACA ACTATTTCGA TGAGGAGACG GTGACGATTT CCCCCAGTCC CAAGAAGGCC 
AAGAGCAACC CCAAGCTGCA GCCCATTGTG GATGCCACCA TCACGAAGTC CAAGAGCAAA 
TCCAACGACC AAAAGACCAA AGAGAGTGCT AACGATATCG ACAGCGACTC TCAATATGAC 
TACGACGAGG GTCTGGATGA TGATGATGAC GAGGACGATG CCGCTGACGA CCTTTTGACC 
GATGATGAGC AAGTTGTCTT CAGCGAGGAC GTTCCGTGTC CAAGATTCTG CCAGTGTGCC 
CGGAATGTCA ACAGCTATCT GGTGGCCACC TGCAGCAGAC TTGACATGGG CATCCAGAAG 
TTTGGCTCTG ACATTACGGA TTTGGTTGTG ACCAATGTGG GTCCTAAGTA CCCCATCCTG 
ATGGGACCCA ACTTCTTCCA GAATCTGGGT CTTAAGAACG TGGCTTCCAT CAAGATCGCC 
AACTGCACCT TGGAGTACCT GCACGCCGAG GCCTTCCACG GACTGAACGA GCTGTACGCT 
GTTAACTTGA CGGATGTTGG TTTGGCTATC ATTAATCCCG ATACCTTTGT TGGAAACAAG 
AAACTGCGCA TGCTGACCAT TTCGGGTAAC GACTTGAGTG TGATGTCCAG CATTCACTAT 
TTGCTGAAGT CTTCCAGCAT TGAGGAGCTG GACTTCTCGC GCAACAACCT TATGGAGTTG 
AATCCCAAGG CCTTCTCCCA TCTATCCAAT GTCGTCTACA TTAACTTGAG CCAGAACAGT 
CTCAAGAAAC TGCCCGAGAA GGCTTTCGAG AAGGTTACTC TTCTGGAAGA ATTGGATCTG 
TCTTACAACT CGCTGACTGA ATTGCCCCGC GATATCTTCA ATGGCACCAC CCTGTCCATA 
CTGCACCTGA AGTACAATAC CTTCAATGGC GATCTGCACT TTGGCACCAA GGATCTGCAG 
CAGCTGGACT TGAGCTTTAA CTCGATTGTC CAAGTGCACC ACAGCATGTT CGACAAGATG 
CCCGGTCTGA CCAACCTGAA CCTCAAGGGC AATGGCATTA AGAAGATCCA ACCGGACTCG 
TTCCTTACTT TGAAGAACCT GCGCCACATC GATTTGTCCA TCAACGATCT GGACCAGATC 
TCGGGAATGC TGTTCTTTAA GAACTCTGAG CTGGATGTGA TCCGTTTGAA CGACAATCCT 
CGTCTGAGCC AGTTGCCCAC CGATGGCTTC TTGAGCTACA GTGGGGAGTT CACCGTCTAC 
TACCTGGATA TTTCCAATTG CGCCATCGGT CCATTGGGTC ACAAGGCCTT CTCCACTATG 
CCGCATTTGA CCACCTTGAA GTTGGCCTGG AACAACATTA ATCATCTGCC GCGCGAGATT 
TTCACTGGAC TGCACAAGCT GATTGACTTG GATCTGAGCA ACAACCTGAT CACCCGCATG 
GACGATCTGA TCTTCATGGA CAACGGAGAG CTGACTAAGC TCAGTCTGGC TGGAAATCCC 
ATTAGCCGCT TGTCCGTCCG CCTCTTCCTG CCGCTGCACC AACTGCGATG CCTGGACGTC 
AATGACTGCG AGCTTACCAC CTTGCTCTCC GATCGCGACT TGGGTGCGGG TTACAAGATC 
TTTGACAGCC TGCGCTCCTT TAACGCCTCT GGTAATTTGA TCAAGAAGAT CTCTTCGGAG 
GATGTGAAGA GCTTCAAGAA CCTGCGTTCT CTGGATATCA CCAACAATCC GCTGAAGTGC 
ACTCCCGACT TCCAGGAGTT CATCAGCTAC GTCACTCTTC AGATGCAGAT GACTCCCAAG 
CGTCTGCCCG TGCTGGCCAA TCTGGAGGAC GATGCTACTA TTGTGCAGCT GGAGACTCTG 
GCTCAGGCTG GTTGGTCGTC TTTGGCCCAC GAGGTGTGCA AGCATGCCGA AGGATCAGAT 
CTCTTGGACG AAAAGAAGGC CGACAGTGCC GAAGCCAAAC TGGAGAAGCG TCTGAAGGAA 
AGCGTGAAGA AGCTTAACGA GGATGAGGCT AAGTTGCTGA GTGTCCTGGA CAAGAGTGTG 
CTAGACAAGA GCAGGCTGAG CAGCATGCAG AAGATCATGA AGGGATCGGT GAACACTGAA 
ACCGCGAAGC CTGAGGAAGA TGCTGACGAG GAGAATCTGA ACAATGGCGG CGAGGATAAG 
GAGGATGACA GCAGTGACTA TGACAGTGAG GAGGATGATG ATGACGATGA TGAGGACGAT 
GATGATGACA ACAACGATGA TGATCGCTTC GAGGAGGCCA AGAAGAATGC CAAGGCTAAG 
GCCGATGCTA ACAAGGCCTT CGACACGTTC GTGAACAAGG AACTGAAGCC AGTCCTGAAG 
ACCGATGGTA TCCAGGCGGA GGAAGTGGAC ATGTCTCAGG AGACCCGTGA TAAATTCCTT 
CTGGAGAAAC TGGGCTTCGA CAGCGAAAGC GAGGAGTCAG ATGAGTACAC CGAGAAGATG 
ATCTTCCACG GCGAACTATC CTACGACCTG GTGGTCCCAC TGATCGTCGT GTCCTTCTGC 
CTCCTGATGA TCGTGCTGTC CATCGCCCGA GTGATCTACG TGGTGCTGCG CAAGCGCGGC 
GAGCGCTATC GCATGGCGCT GCTGGCCTCA AAGAACTCGT TCGTATACCA GAAGTTGAGC 
GAGGACATCG TGAAGCCGAC CAGCAAGGAG CAGAAGGAGC CCAAGCATCT GAAGCAGCCA 
AAGGTGCACC GATACGCGCC CATCAATCAG GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726190.1
CDS	169..3324
Translation	MSRQSTCRLVLLLAFTLALIHASHSNTVGPKIHFIHDDLTENELNSGEEVSPTESLPKISTPKVKDLPVETTTRSPTKKTTSSGTEKSLSEEAWNDLMEAAKHSQALADDPHFNKMLNTTVAAGEKNSTDTDDSEYYDYDDDYNYFDEETVTISPSPKKAKSNPKLQPIVDATITKSKSKSNDQKTKESANDIDSDSQYDYDEGLDDDDDEDDAADDLLTDDEQVVFSEDVPCPRFCQCARNVNSYLVATCSRLDMGIQKFGSDITDLVVTNVGPKYPILMGPNFFQNLGLKNVASIKIANCTLEYLHAEAFHGLNELYAVNLTDVGLAIINPDTFVGNKKLRMLTISGNDLSVMSSIHYLLKSSSIEELDFSRNNLMELNPKAFSHLSNVVYINLSQNSLKKLPEKAFEKVTLLEELDLSYNSLTELPRDIFNGTTLSILHLKYNTFNGDLHFGTKDLQQLDLSFNSIVQVHHSMFDKMPGLTNLNLKGNGIKKIQPDSFLTLKNLRHIDLSINDLDQISGMLFFKNSELDVIRLNDNPRLSQLPTDGFLSYSGEFTVYYLDISNCAIGPLGHKAFSTMPHLTTLKLAWNNINHLPREIFTGLHKLIDLDLSNNLITRMDDLIFMDNGELTKLSLAGNPISRLSVRLFLPLHQLRCLDVNDCELTTLLSDRDLGAGYKIFDSLRSFNASGNLIKKISSEDVKSFKNLRSLDITNNPLKCTPDFQEFISYVTLQMQMTPKRLPVLANLEDDATIVQLETLAQAGWSSLAHEVCKHAEGSDLLDEKKADSAEAKLEKRLKESVKKLNEDEAKLLSVLDKSVLDKSRLSSMQKIMKGSVNTETAKPEEDADEENLNNGGEDKEDDSSDYDSEEDDDDDDEDDDDDNNDDDRFEEAKKNAKAKADANKAFDTFVNKELKPVLKTDGIQAEEVDMSQETRDKFLLEKLGFDSESEESDEYTEKMIFHGELSYDLVVPLIVVSFCLLMIVLSIARVIYVVLRKRGERYRMALLASKNSFVYQKLSEDIVKPTSKEQKEPKHLKQPKVHRYAPINQV

Target ORF information:

RefSeq Version	NM_166527.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Gp150, transcript variant A (Gp150), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166527.2

ORF Insert Sequence:

ATGAGTCGCC AGTCGACTTG CAGGCTAGTC CTGCTGCTGG CCTTTACCCT GGCCCTAATC 
CACGCCAGCC ACTCAAATAC CGTGGGCCCC AAGATCCACT TCATTCATGA CGATCTGACG 
GAAAACGAGC TGAACTCTGG AGAGGAGGTG AGCCCGACCG AGAGTCTGCC CAAAATCTCC 
ACCCCCAAGG TAAAGGATTT ACCCGTGGAG ACGACCACCA GGAGCCCCAC AAAAAAAACC 
ACGTCCTCCG GCACCGAGAA GTCCCTTAGC GAGGAGGCTT GGAATGATCT GATGGAGGCG 
GCCAAGCACA GCCAAGCTTT GGCTGATGAT CCACACTTCA ACAAGATGCT CAATACTACC 
GTCGCCGCCG GGGAGAAGAA CTCCACCGAT ACCGACGATA GCGAATACTA TGACTACGAT 
GATGATTACA ACTATTTCGA TGAGGAGACG GTGACGATTT CCCCCAGTCC CAAGAAGGCC 
AAGAGCAACC CCAAGCTGCA GCCCATTGTG GATGCCACCA TCACGAAGTC CAAGAGCAAA 
TCCAACGACC AAAAGACCAA AGAGAGTGCT AACGATATCG ACAGCGACTC TCAATATGAC 
TACGACGAGG GTCTGGATGA TGATGATGAC GAGGACGATG CCGCTGACGA CCTTTTGACC 
GATGATGAGC AAGTTGTCTT CAGCGAGGAC GTTCCGTGTC CAAGATTCTG CCAGTGTGCC 
CGGAATGTCA ACAGCTATCT GGTGGCCACC TGCAGCAGAC TTGACATGGG CATCCAGAAG 
TTTGGCTCTG ACATTACGGA TTTGGTTGTG ACCAATGTGG GTCCTAAGTA CCCCATCCTG 
ATGGGACCCA ACTTCTTCCA GAATCTGGGT CTTAAGAACG TGGCTTCCAT CAAGATCGCC 
AACTGCACCT TGGAGTACCT GCACGCCGAG GCCTTCCACG GACTGAACGA GCTGTACGCT 
GTTAACTTGA CGGATGTTGG TTTGGCTATC ATTAATCCCG ATACCTTTGT TGGAAACAAG 
AAACTGCGCA TGCTGACCAT TTCGGGTAAC GACTTGAGTG TGATGTCCAG CATTCACTAT 
TTGCTGAAGT CTTCCAGCAT TGAGGAGCTG GACTTCTCGC GCAACAACCT TATGGAGTTG 
AATCCCAAGG CCTTCTCCCA TCTATCCAAT GTCGTCTACA TTAACTTGAG CCAGAACAGT 
CTCAAGAAAC TGCCCGAGAA GGCTTTCGAG AAGGTTACTC TTCTGGAAGA ATTGGATCTG 
TCTTACAACT CGCTGACTGA ATTGCCCCGC GATATCTTCA ATGGCACCAC CCTGTCCATA 
CTGCACCTGA AGTACAATAC CTTCAATGGC GATCTGCACT TTGGCACCAA GGATCTGCAG 
CAGCTGGACT TGAGCTTTAA CTCGATTGTC CAAGTGCACC ACAGCATGTT CGACAAGATG 
CCCGGTCTGA CCAACCTGAA CCTCAAGGGC AATGGCATTA AGAAGATCCA ACCGGACTCG 
TTCCTTACTT TGAAGAACCT GCGCCACATC GATTTGTCCA TCAACGATCT GGACCAGATC 
TCGGGAATGC TGTTCTTTAA GAACTCTGAG CTGGATGTGA TCCGTTTGAA CGACAATCCT 
CGTCTGAGCC AGTTGCCCAC CGATGGCTTC TTGAGCTACA GTGGGGAGTT CACCGTCTAC 
TACCTGGATA TTTCCAATTG CGCCATCGGT CCATTGGGTC ACAAGGCCTT CTCCACTATG 
CCGCATTTGA CCACCTTGAA GTTGGCCTGG AACAACATTA ATCATCTGCC GCGCGAGATT 
TTCACTGGAC TGCACAAGCT GATTGACTTG GATCTGAGCA ACAACCTGAT CACCCGCATG 
GACGATCTGA TCTTCATGGA CAACGGAGAG CTGACTAAGC TCAGTCTGGC TGGAAATCCC 
ATTAGCCGCT TGTCCGTCCG CCTCTTCCTG CCGCTGCACC AACTGCGATG CCTGGACGTC 
AATGACTGCG AGCTTACCAC CTTGCTCTCC GATCGCGACT TGGGTGCGGG TTACAAGATC 
TTTGACAGCC TGCGCTCCTT TAACGCCTCT GGTAATTTGA TCAAGAAGAT CTCTTCGGAG 
GATGTGAAGA GCTTCAAGAA CCTGCGTTCT CTGGATATCA CCAACAATCC GCTGAAGTGC 
ACTCCCGACT TCCAGGAGTT CATCAGCTAC GTCACTCTTC AGATGCAGAT GACTCCCAAG 
CGTCTGCCCG TGCTGGCCAA TCTGGAGGAC GATGCTACTA TTGTGCAGCT GGAGACTCTG 
GCTCAGGCTG GTTGGTCGTC TTTGGCCCAC GAGGTGTGCA AGCATGCCGA AGGATCAGAT 
CTCTTGGACG AAAAGAAGGC CGACAGTGCC GAAGCCAAAC TGGAGAAGCG TCTGAAGGAA 
AGCGTGAAGA AGCTTAACGA GGATGAGGCT AAGTTGCTGA GTGTCCTGGA CAAGAGTGTG 
CTAGACAAGA GCAGGCTGAG CAGCATGCAG AAGATCATGA AGGGATCGGT GAACACTGAA 
ACCGCGAAGC CTGAGGAAGA TGCTGACGAG GAGAATCTGA ACAATGGCGG CGAGGATAAG 
GAGGATGACA GCAGTGACTA TGACAGTGAG GAGGATGATG ATGACGATGA TGAGGACGAT 
GATGATGACA ACAACGATGA TGATCGCTTC GAGGAGGCCA AGAAGAATGC CAAGGCTAAG 
GCCGATGCTA ACAAGGCCTT CGACACGTTC GTGAACAAGG AACTGAAGCC AGTCCTGAAG 
ACCGATGGTA TCCAGGCGGA GGAAGTGGAC ATGTCTCAGG AGACCCGTGA TAAATTCCTT 
CTGGAGAAAC TGGGCTTCGA CAGCGAAAGC GAGGAGTCAG ATGAGTACAC CGAGAAGATG 
ATCTTCCACG GCGAACTATC CTACGACCTG GTGGTCCCAC TGATCGTCGT GTCCTTCTGC 
CTCCTGATGA TCGTGCTGTC CATCGCCCGA GTGATCTACG TGGTGCTGCG CAAGCGCGGC 
GAGCGCTATC GCATGGCGCT GCTGGCCTCA AAGAACTCGT TCGTATACCA GAAGTTGAGC 
GAGGACATCG TGAAGCCGAC CAGCAAGGAG CAGAAGGAGC CCAAGCATCT GAAGCAGCCA 
AAGGTGCACC GATACGCGCC CATCAATCAG GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gp150 ( NM_057701.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477049.2 Drosophila melanogaster Gp150, transcript variant D (Gp150), mRNA.

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CloneID	OFa10913
Clone ID Related Accession (Same CDS sequence)	NM_057701.4
Accession Version	NM_057701.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Gp150, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057701.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCACAATT TGATAACCGG GCCCCATCTG CTCTCCAATC CACATGACAT TTCTGGTTTG 
CCCATCTACA GGAAAATGAG TCGCCAGTCG ACTTGCAGGC TAGTCCTGCT GCTGGCCTTT 
ACCCTGGCCC TAATCCACGC CAGCCACTCA AATACCGTGG GCCCCAAGAT CCACTTCATT 
CATGACGATC TGACGGAAAA CGAGCTGAAC TCTGGAGAGG AGGTGAGCCC GACCGAGAGT 
CTGCCCAAAA TCTCCACCCC CAAGGTAAAG GATTTACCCG TGGAGACGAC CACCAGGAGC 
CCCACAAAAA AAACCACGTC CTCCGGCACC GAGAAGTCCC TTAGCGAGGA GGCTTGGAAT 
GATCTGATGG AGGCGGCCAA GCACAGCCAA GCTTTGGCTG ATGATCCACA CTTCAACAAG 
ATGCTCAATA CTACCGTCGC CGCCGGGGAG AAGAACTCCA CCGATACCGA CGATAGCGAA 
TACTATGACT ACGATGATGA TTACAACTAT TTCGATGAGG AGACGGTGAC GATTTCCCCC 
AGTCCCAAGA AGGCCAAGAG CAACCCCAAG CTGCAGCCCA TTGTGGATGC CACCATCACG 
AAGTCCAAGA GCAAATCCAA CGACCAAAAG ACCAAAGAGA GTGCTAACGA TATCGACAGC 
GACTCTCAAT ATGACTACGA CGAGGGTCTG GATGATGATG ATGACGAGGA CGATGCCGCT 
GACGACCTTT TGACCGATGA TGAGCAAGTT GTCTTCAGCG AGGACGTTCC GTGTCCAAGA 
TTCTGCCAGT GTGCCCGGAA TGTCAACAGC TATCTGGTGG CCACCTGCAG CAGACTTGAC 
ATGGGCATCC AGAAGTTTGG CTCTGACATT ACGGATTTGG TTGTGACCAA TGTGGGTCCT 
AAGTACCCCA TCCTGATGGG ACCCAACTTC TTCCAGAATC TGGGTCTTAA GAACGTGGCT 
TCCATCAAGA TCGCCAACTG CACCTTGGAG TACCTGCACG CCGAGGCCTT CCACGGACTG 
AACGAGCTGT ACGCTGTTAA CTTGACGGAT GTTGGTTTGG CTATCATTAA TCCCGATACC 
TTTGTTGGAA ACAAGAAACT GCGCATGCTG ACCATTTCGG GTAACGACTT GAGTGTGATG 
TCCAGCATTC ACTATTTGCT GAAGTCTTCC AGCATTGAGG AGCTGGACTT CTCGCGCAAC 
AACCTTATGG AGTTGAATCC CAAGGCCTTC TCCCATCTAT CCAATGTCGT CTACATTAAC 
TTGAGCCAGA ACAGTCTCAA GAAACTGCCC GAGAAGGCTT TCGAGAAGGT TACTCTTCTG 
GAAGAATTGG ATCTGTCTTA CAACTCGCTG ACTGAATTGC CCCGCGATAT CTTCAATGGC 
ACCACCCTGT CCATACTGCA CCTGAAGTAC AATACCTTCA ATGGCGATCT GCACTTTGGC 
ACCAAGGATC TGCAGCAGCT GGACTTGAGC TTTAACTCGA TTGTCCAAGT GCACCACAGC 
ATGTTCGACA AGATGCCCGG TCTGACCAAC CTGAACCTCA AGGGCAATGG CATTAAGAAG 
ATCCAACCGG ACTCGTTCCT TACTTTGAAG AACCTGCGCC ACATCGATTT GTCCATCAAC 
GATCTGGACC AGATCTCGGG AATGCTGTTC TTTAAGAACT CTGAGCTGGA TGTGATCCGT 
TTGAACGACA ATCCTCGTCT GAGCCAGTTG CCCACCGATG GCTTCTTGAG CTACAGTGGG 
GAGTTCACCG TCTACTACCT GGATATTTCC AATTGCGCCA TCGGTCCATT GGGTCACAAG 
GCCTTCTCCA CTATGCCGCA TTTGACCACC TTGAAGTTGG CCTGGAACAA CATTAATCAT 
CTGCCGCGCG AGATTTTCAC TGGACTGCAC AAGCTGATTG ACTTGGATCT GAGCAACAAC 
CTGATCACCC GCATGGACGA TCTGATCTTC ATGGACAACG GAGAGCTGAC TAAGCTCAGT 
CTGGCTGGAA ATCCCATTAG CCGCTTGTCC GTCCGCCTCT TCCTGCCGCT GCACCAACTG 
CGATGCCTGG ACGTCAATGA CTGCGAGCTT ACCACCTTGC TCTCCGATCG CGACTTGGGT 
GCGGGTTACA AGATCTTTGA CAGCCTGCGC TCCTTTAACG CCTCTGGTAA TTTGATCAAG 
AAGATCTCTT CGGAGGATGT GAAGAGCTTC AAGAACCTGC GTTCTCTGGA TATCACCAAC 
AATCCGCTGA AGTGCACTCC CGACTTCCAG GAGTTCATCA GCTACGTCAC TCTTCAGATG 
CAGATGACTC CCAAGCGTCT GCCCGTGCTG GCCAATCTGG AGGACGATGC TACTATTGTG 
CAGCTGGAGA CTCTGGCTCA GGCTGGTTGG TCGTCTTTGG CCCACGAGGT GTGCAAGCAT 
GCCGAAGGAT CAGATCTCTT GGACGAAAAG AAGGCCGACA GTGCCGAAGC CAAACTGGAG 
AAGCGTCTGA AGGAAAGCGT GAAGAAGCTT AACGAGGATG AGGCTAAGTT GCTGAGTGTC 
CTGGACAAGA GTGTGCTAGA CAAGAGCAGG CTGAGCAGCA TGCAGAAGAT CATGAAGGGA 
TCGGTGAACA CTGAAACCGC GAAGCCTGAG GAAGATGCTG ACGAGGAGAA TCTGAACAAT 
GGCGGCGAGG ATAAGGAGGA TGACAGCAGT GACTATGACA GTGAGGAGGA TGATGATGAC 
GATGATGAGG ACGATGATGA TGACAACAAC GATGATGATC GCTTCGAGGA GGCCAAGAAG 
AATGCCAAGG CTAAGGCCGA TGCTAACAAG GCCTTCGACA CGTTCGTGAA CAAGGAACTG 
AAGCCAGTCC TGAAGACCGA TGGTATCCAG GCGGAGGAAG TGGACATGTC TCAGGAGACC 
CGTGATAAAT TCCTTCTGGA GAAACTGGGC TTCGACAGCG AAAGCGAGGA GTCAGATGAG 
TACACCGAGA AGATGATCTT CCACGGCGAA CTATCCTACG ACCTGGTGGT CCCACTGATC 
GTCGTGTCCT TCTGCCTCCT GATGATCGTG CTGTCCATCG CCCGAGTGAT CTACGTGGTG 
CTGCGCAAGC GCGGCGAGCG CTATCGCATG GCGCTGCTGG CCTCAAAGAA CTCGTTCGTA 
TACCAGAAGT TGAGCGAGGA CATCGTGAAG CCGACCAGCA AGGAGCAGAA GGAGCCCAAG 
CATCTGAAGC AGCCAAAGGT GCACCGATAC GCGCCCATCA ATCAGGTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477049.2
CDS	68..3298
Translation	MHNLITGPHLLSNPHDISGLPIYRKMSRQSTCRLVLLLAFTLALIHASHSNTVGPKIHFIHDDLTENELNSGEEVSPTESLPKISTPKVKDLPVETTTRSPTKKTTSSGTEKSLSEEAWNDLMEAAKHSQALADDPHFNKMLNTTVAAGEKNSTDTDDSEYYDYDDDYNYFDEETVTISPSPKKAKSNPKLQPIVDATITKSKSKSNDQKTKESANDIDSDSQYDYDEGLDDDDDEDDAADDLLTDDEQVVFSEDVPCPRFCQCARNVNSYLVATCSRLDMGIQKFGSDITDLVVTNVGPKYPILMGPNFFQNLGLKNVASIKIANCTLEYLHAEAFHGLNELYAVNLTDVGLAIINPDTFVGNKKLRMLTISGNDLSVMSSIHYLLKSSSIEELDFSRNNLMELNPKAFSHLSNVVYINLSQNSLKKLPEKAFEKVTLLEELDLSYNSLTELPRDIFNGTTLSILHLKYNTFNGDLHFGTKDLQQLDLSFNSIVQVHHSMFDKMPGLTNLNLKGNGIKKIQPDSFLTLKNLRHIDLSINDLDQISGMLFFKNSELDVIRLNDNPRLSQLPTDGFLSYSGEFTVYYLDISNCAIGPLGHKAFSTMPHLTTLKLAWNNINHLPREIFTGLHKLIDLDLSNNLITRMDDLIFMDNGELTKLSLAGNPISRLSVRLFLPLHQLRCLDVNDCELTTLLSDRDLGAGYKIFDSLRSFNASGNLIKKISSEDVKSFKNLRSLDITNNPLKCTPDFQEFISYVTLQMQMTPKRLPVLANLEDDATIVQLETLAQAGWSSLAHEVCKHAEGSDLLDEKKADSAEAKLEKRLKESVKKLNEDEAKLLSVLDKSVLDKSRLSSMQKIMKGSVNTETAKPEEDADEENLNNGGEDKEDDSSDYDSEEDDDDDDEDDDDDNNDDDRFEEAKKNAKAKADANKAFDTFVNKELKPVLKTDGIQAEEVDMSQETRDKFLLEKLGFDSESEESDEYTEKMIFHGELSYDLVVPLIVVSFCLLMIVLSIARVIYVVLRKRGERYRMALLASKNSFVYQKLSEDIVKPTSKEQKEPKHLKQPKVHRYAPINQV

Target ORF information:

RefSeq Version	NM_057701.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Gp150, transcript variant D (Gp150), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057701.4

ORF Insert Sequence:

ATGCACAATT TGATAACCGG GCCCCATCTG CTCTCCAATC CACATGACAT TTCTGGTTTG 
CCCATCTACA GGAAAATGAG TCGCCAGTCG ACTTGCAGGC TAGTCCTGCT GCTGGCCTTT 
ACCCTGGCCC TAATCCACGC CAGCCACTCA AATACCGTGG GCCCCAAGAT CCACTTCATT 
CATGACGATC TGACGGAAAA CGAGCTGAAC TCTGGAGAGG AGGTGAGCCC GACCGAGAGT 
CTGCCCAAAA TCTCCACCCC CAAGGTAAAG GATTTACCCG TGGAGACGAC CACCAGGAGC 
CCCACAAAAA AAACCACGTC CTCCGGCACC GAGAAGTCCC TTAGCGAGGA GGCTTGGAAT 
GATCTGATGG AGGCGGCCAA GCACAGCCAA GCTTTGGCTG ATGATCCACA CTTCAACAAG 
ATGCTCAATA CTACCGTCGC CGCCGGGGAG AAGAACTCCA CCGATACCGA CGATAGCGAA 
TACTATGACT ACGATGATGA TTACAACTAT TTCGATGAGG AGACGGTGAC GATTTCCCCC 
AGTCCCAAGA AGGCCAAGAG CAACCCCAAG CTGCAGCCCA TTGTGGATGC CACCATCACG 
AAGTCCAAGA GCAAATCCAA CGACCAAAAG ACCAAAGAGA GTGCTAACGA TATCGACAGC 
GACTCTCAAT ATGACTACGA CGAGGGTCTG GATGATGATG ATGACGAGGA CGATGCCGCT 
GACGACCTTT TGACCGATGA TGAGCAAGTT GTCTTCAGCG AGGACGTTCC GTGTCCAAGA 
TTCTGCCAGT GTGCCCGGAA TGTCAACAGC TATCTGGTGG CCACCTGCAG CAGACTTGAC 
ATGGGCATCC AGAAGTTTGG CTCTGACATT ACGGATTTGG TTGTGACCAA TGTGGGTCCT 
AAGTACCCCA TCCTGATGGG ACCCAACTTC TTCCAGAATC TGGGTCTTAA GAACGTGGCT 
TCCATCAAGA TCGCCAACTG CACCTTGGAG TACCTGCACG CCGAGGCCTT CCACGGACTG 
AACGAGCTGT ACGCTGTTAA CTTGACGGAT GTTGGTTTGG CTATCATTAA TCCCGATACC 
TTTGTTGGAA ACAAGAAACT GCGCATGCTG ACCATTTCGG GTAACGACTT GAGTGTGATG 
TCCAGCATTC ACTATTTGCT GAAGTCTTCC AGCATTGAGG AGCTGGACTT CTCGCGCAAC 
AACCTTATGG AGTTGAATCC CAAGGCCTTC TCCCATCTAT CCAATGTCGT CTACATTAAC 
TTGAGCCAGA ACAGTCTCAA GAAACTGCCC GAGAAGGCTT TCGAGAAGGT TACTCTTCTG 
GAAGAATTGG ATCTGTCTTA CAACTCGCTG ACTGAATTGC CCCGCGATAT CTTCAATGGC 
ACCACCCTGT CCATACTGCA CCTGAAGTAC AATACCTTCA ATGGCGATCT GCACTTTGGC 
ACCAAGGATC TGCAGCAGCT GGACTTGAGC TTTAACTCGA TTGTCCAAGT GCACCACAGC 
ATGTTCGACA AGATGCCCGG TCTGACCAAC CTGAACCTCA AGGGCAATGG CATTAAGAAG 
ATCCAACCGG ACTCGTTCCT TACTTTGAAG AACCTGCGCC ACATCGATTT GTCCATCAAC 
GATCTGGACC AGATCTCGGG AATGCTGTTC TTTAAGAACT CTGAGCTGGA TGTGATCCGT 
TTGAACGACA ATCCTCGTCT GAGCCAGTTG CCCACCGATG GCTTCTTGAG CTACAGTGGG 
GAGTTCACCG TCTACTACCT GGATATTTCC AATTGCGCCA TCGGTCCATT GGGTCACAAG 
GCCTTCTCCA CTATGCCGCA TTTGACCACC TTGAAGTTGG CCTGGAACAA CATTAATCAT 
CTGCCGCGCG AGATTTTCAC TGGACTGCAC AAGCTGATTG ACTTGGATCT GAGCAACAAC 
CTGATCACCC GCATGGACGA TCTGATCTTC ATGGACAACG GAGAGCTGAC TAAGCTCAGT 
CTGGCTGGAA ATCCCATTAG CCGCTTGTCC GTCCGCCTCT TCCTGCCGCT GCACCAACTG 
CGATGCCTGG ACGTCAATGA CTGCGAGCTT ACCACCTTGC TCTCCGATCG CGACTTGGGT 
GCGGGTTACA AGATCTTTGA CAGCCTGCGC TCCTTTAACG CCTCTGGTAA TTTGATCAAG 
AAGATCTCTT CGGAGGATGT GAAGAGCTTC AAGAACCTGC GTTCTCTGGA TATCACCAAC 
AATCCGCTGA AGTGCACTCC CGACTTCCAG GAGTTCATCA GCTACGTCAC TCTTCAGATG 
CAGATGACTC CCAAGCGTCT GCCCGTGCTG GCCAATCTGG AGGACGATGC TACTATTGTG 
CAGCTGGAGA CTCTGGCTCA GGCTGGTTGG TCGTCTTTGG CCCACGAGGT GTGCAAGCAT 
GCCGAAGGAT CAGATCTCTT GGACGAAAAG AAGGCCGACA GTGCCGAAGC CAAACTGGAG 
AAGCGTCTGA AGGAAAGCGT GAAGAAGCTT AACGAGGATG AGGCTAAGTT GCTGAGTGTC 
CTGGACAAGA GTGTGCTAGA CAAGAGCAGG CTGAGCAGCA TGCAGAAGAT CATGAAGGGA 
TCGGTGAACA CTGAAACCGC GAAGCCTGAG GAAGATGCTG ACGAGGAGAA TCTGAACAAT 
GGCGGCGAGG ATAAGGAGGA TGACAGCAGT GACTATGACA GTGAGGAGGA TGATGATGAC 
GATGATGAGG ACGATGATGA TGACAACAAC GATGATGATC GCTTCGAGGA GGCCAAGAAG 
AATGCCAAGG CTAAGGCCGA TGCTAACAAG GCCTTCGACA CGTTCGTGAA CAAGGAACTG 
AAGCCAGTCC TGAAGACCGA TGGTATCCAG GCGGAGGAAG TGGACATGTC TCAGGAGACC 
CGTGATAAAT TCCTTCTGGA GAAACTGGGC TTCGACAGCG AAAGCGAGGA GTCAGATGAG 
TACACCGAGA AGATGATCTT CCACGGCGAA CTATCCTACG ACCTGGTGGT CCCACTGATC 
GTCGTGTCCT TCTGCCTCCT GATGATCGTG CTGTCCATCG CCCGAGTGAT CTACGTGGTG 
CTGCGCAAGC GCGGCGAGCG CTATCGCATG GCGCTGCTGG CCTCAAAGAA CTCGTTCGTA 
TACCAGAAGT TGAGCGAGGA CATCGTGAAG CCGACCAGCA AGGAGCAGAA GGAGCCCAAG 
CATCTGAAGC AGCCAAAGGT GCACCGATAC GCGCCCATCA ATCAGGTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gp150 ( NM_001299784.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286713.1 Drosophila melanogaster Gp150, transcript variant E (Gp150), mRNA.

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CloneID	OFa21911
Clone ID Related Accession (Same CDS sequence)	NM_001299784.1
Accession Version	NM_001299784.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Gp150, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTCGCC AGTCGACTTG CAGGCTAGTC CTGCTGCTGG CCTTTACCCT GGCCCTAATC 
CACGCCAGCC ACTCAAATAC CGTGGGCCCC AAGATCCACT TCATTCATGA CGATCTGACG 
GAAAACGAGC TGAACTCTGG AGAGGAGGTG AGCCCGACCG AGAGTCTGCC CAAAATCTCC 
ACCCCCAAGG TAAAGGATTT ACCCGTGGAG ACGACCACCA GGAGCCCCAC AAAAAAAACC 
ACGTCCTCCG GCACCGAGAA GTCCCTTAGC GAGGAGGCTT GGAATGATCT GATGGAGGCG 
GCCAAGCACA GCCAAGCTTT GGCTGATGAT CCACACTTCA ACAAGATGCT CAATACTACC 
GTCGCCGCCG GGGAGAAGAA CTCCACCGAT ACCGACGATA GCGAATACTA TGACTACGAT 
GATGATTACA ACTATTTCGA TGAGGAGACG GTGACGATTT CCCCCAGTCC CAAGAAGGCC 
AAGAGCAACC CCAAGCTGCA GCCCATTGTG GATGCCACCA TCACGAAGTC CAAGACCAAA 
GAGAGTGCTA ACGATATCGA CAGCGACTCT CAATATGACT ACGACGAGGG TCTGGATGAT 
GATGATGACG AGGACGATGC CGCTGACGAC CTTTTGACCG ATGATGAGCA AGTTGTCTTC 
AGCGAGGACG TTCCGTGTCC AAGATTCTGC CAGTGTGCCC GGAATGTCAA CAGCTATCTG 
GTGGCCACCT GCAGCAGACT TGACATGGGC ATCCAGAAGT TTGGCTCTGA CATTACGGAT 
TTGGTTGTGA CCAATGTGGG TCCTAAGTAC CCCATCCTGA TGGGACCCAA CTTCTTCCAG 
AATCTGGGTC TTAAGAACGT GGCTTCCATC AAGATCGCCA ACTGCACCTT GGAGTACCTG 
CACGCCGAGG CCTTCCACGG ACTGAACGAG CTGTACGCTG TTAACTTGAC GGATGTTGGT 
TTGGCTATCA TTAATCCCGA TACCTTTGTT GGAAACAAGA AACTGCGCAT GCTGACCATT 
TCGGGTAACG ACTTGAGTGT GATGTCCAGC ATTCACTATT TGCTGAAGTC TTCCAGCATT 
GAGGAGCTGG ACTTCTCGCG CAACAACCTT ATGGAGTTGA ATCCCAAGGC CTTCTCCCAT 
CTATCCAATG TCGTCTACAT TAACTTGAGC CAGAACAGTC TCAAGAAACT GCCCGAGAAG 
GCTTTCGAGA AGGTTACTCT TCTGGAAGAA TTGGATCTGT CTTACAACTC GCTGACTGAA 
TTGCCCCGCG ATATCTTCAA TGGCACCACC CTGTCCATAC TGCACCTGAA GTACAATACC 
TTCAATGGCG ATCTGCACTT TGGCACCAAG GATCTGCAGC AGCTGGACTT GAGCTTTAAC 
TCGATTGTCC AAGTGCACCA CAGCATGTTC GACAAGATGC CCGGTCTGAC CAACCTGAAC 
CTCAAGGGCA ATGGCATTAA GAAGATCCAA CCGGACTCGT TCCTTACTTT GAAGAACCTG 
CGCCACATCG ATTTGTCCAT CAACGATCTG GACCAGATCT CGGGAATGCT GTTCTTTAAG 
AACTCTGAGC TGGATGTGAT CCGTTTGAAC GACAATCCTC GTCTGAGCCA GTTGCCCACC 
GATGGCTTCT TGAGCTACAG TGGGGAGTTC ACCGTCTACT ACCTGGATAT TTCCAATTGC 
GCCATCGGTC CATTGGGTCA CAAGGCCTTC TCCACTATGC CGCATTTGAC CACCTTGAAG 
TTGGCCTGGA ACAACATTAA TCATCTGCCG CGCGAGATTT TCACTGGACT GCACAAGCTG 
ATTGACTTGG ATCTGAGCAA CAACCTGATC ACCCGCATGG ACGATCTGAT CTTCATGGAC 
AACGGAGAGC TGACTAAGCT CAGTCTGGCT GGAAATCCCA TTAGCCGCTT GTCCGTCCGC 
CTCTTCCTGC CGCTGCACCA ACTGCGATGC CTGGACGTCA ATGACTGCGA GCTTACCACC 
TTGCTCTCCG ATCGCGACTT GGGTGCGGGT TACAAGATCT TTGACAGCCT GCGCTCCTTT 
AACGCCTCTG GTAATTTGAT CAAGAAGATC TCTTCGGAGG ATGTGAAGAG CTTCAAGAAC 
CTGCGTTCTC TGGATATCAC CAACAATCCG CTGAAGTGCA CTCCCGACTT CCAGGAGTTC 
ATCAGCTACG TCACTCTTCA GATGCAGATG ACTCCCAAGC GTCTGCCCGT GCTGGCCAAT 
CTGGAGGACG ATGCTACTAT TGTGCAGCTG GAGACTCTGG CTCAGGCTGG TTGGTCGTCT 
TTGGCCCACG AGGTGTGCAA GCATGCCGAA GGATCAGATC TCTTGGACGA AAAGAAGGCC 
GACAGTGCCG AAGCCAAACT GGAGAAGCGT CTGAAGGAAA GCGTGAAGAA GCTTAACGAG 
GATGAGGCTA AGTTGCTGAG TGTCCTGGAC AAGAGTGTGC TAGACAAGAG CAGGCTGAGC 
AGCATGCAGA AGATCATGAA GGGATCGGTG AACACTGAAA CCGCGAAGCC TGAGGAAGAT 
GCTGACGAGG AGAATCTGAA CAATGGCGGC GAGGATAAGG AGGATGACAG CAGTGACTAT 
GACAGTGAGG AGGATGATGA TGACGATGAT GAGGACGATG ATGATGACAA CAACGATGAT 
GATCGCTTCG AGGAGGCCAA GAAGAATGCC AAGGCTAAGG CCGATGCTAA CAAGGCCTTC 
GACACGTTCG TGAACAAGGA ACTGAAGCCA GTCCTGAAGA CCGATGGTAT CCAGGCGGAG 
GAAGTGGACA TGTCTCAGGA GACCCGTGAT AAATTCCTTC TGGAGAAACT GGGCTTCGAC 
AGCGAAAGCG AGGAGTCAGA TGAGTACACC GAGAAGATGA TCTTCCACGG CGAACTATCC 
TACGACCTGG TGGTCCCACT GATCGTCGTG TCCTTCTGCC TCCTGATGAT CGTGCTGTCC 
ATCGCCCGAG TGATCTACGT GGTGCTGCGC AAGCGCGGCG AGCGCTATCG CATGGCGCTG 
CTGGCCTCAA AGAACTCGTT CGTATACCAG AAGTTGAGCG AGGACATCGT GAAGCCGACC 
AGCAAGGAGC AGAAGGAGCC CAAGCATCTG AAGCAGCCAA AGGTGCACCG ATACGCGCCC 
ATCAATCAGG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286713.1
CDS	396..3530
Translation	MSRQSTCRLVLLLAFTLALIHASHSNTVGPKIHFIHDDLTENELNSGEEVSPTESLPKISTPKVKDLPVETTTRSPTKKTTSSGTEKSLSEEAWNDLMEAAKHSQALADDPHFNKMLNTTVAAGEKNSTDTDDSEYYDYDDDYNYFDEETVTISPSPKKAKSNPKLQPIVDATITKSKTKESANDIDSDSQYDYDEGLDDDDDEDDAADDLLTDDEQVVFSEDVPCPRFCQCARNVNSYLVATCSRLDMGIQKFGSDITDLVVTNVGPKYPILMGPNFFQNLGLKNVASIKIANCTLEYLHAEAFHGLNELYAVNLTDVGLAIINPDTFVGNKKLRMLTISGNDLSVMSSIHYLLKSSSIEELDFSRNNLMELNPKAFSHLSNVVYINLSQNSLKKLPEKAFEKVTLLEELDLSYNSLTELPRDIFNGTTLSILHLKYNTFNGDLHFGTKDLQQLDLSFNSIVQVHHSMFDKMPGLTNLNLKGNGIKKIQPDSFLTLKNLRHIDLSINDLDQISGMLFFKNSELDVIRLNDNPRLSQLPTDGFLSYSGEFTVYYLDISNCAIGPLGHKAFSTMPHLTTLKLAWNNINHLPREIFTGLHKLIDLDLSNNLITRMDDLIFMDNGELTKLSLAGNPISRLSVRLFLPLHQLRCLDVNDCELTTLLSDRDLGAGYKIFDSLRSFNASGNLIKKISSEDVKSFKNLRSLDITNNPLKCTPDFQEFISYVTLQMQMTPKRLPVLANLEDDATIVQLETLAQAGWSSLAHEVCKHAEGSDLLDEKKADSAEAKLEKRLKESVKKLNEDEAKLLSVLDKSVLDKSRLSSMQKIMKGSVNTETAKPEEDADEENLNNGGEDKEDDSSDYDSEEDDDDDDEDDDDDNNDDDRFEEAKKNAKAKADANKAFDTFVNKELKPVLKTDGIQAEEVDMSQETRDKFLLEKLGFDSESEESDEYTEKMIFHGELSYDLVVPLIVVSFCLLMIVLSIARVIYVVLRKRGERYRMALLASKNSFVYQKLSEDIVKPTSKEQKEPKHLKQPKVHRYAPINQV

Target ORF information:

RefSeq Version	NM_001299784.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Gp150, transcript variant E (Gp150), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299784.1

ORF Insert Sequence:

ATGAGTCGCC AGTCGACTTG CAGGCTAGTC CTGCTGCTGG CCTTTACCCT GGCCCTAATC 
CACGCCAGCC ACTCAAATAC CGTGGGCCCC AAGATCCACT TCATTCATGA CGATCTGACG 
GAAAACGAGC TGAACTCTGG AGAGGAGGTG AGCCCGACCG AGAGTCTGCC CAAAATCTCC 
ACCCCCAAGG TAAAGGATTT ACCCGTGGAG ACGACCACCA GGAGCCCCAC AAAAAAAACC 
ACGTCCTCCG GCACCGAGAA GTCCCTTAGC GAGGAGGCTT GGAATGATCT GATGGAGGCG 
GCCAAGCACA GCCAAGCTTT GGCTGATGAT CCACACTTCA ACAAGATGCT CAATACTACC 
GTCGCCGCCG GGGAGAAGAA CTCCACCGAT ACCGACGATA GCGAATACTA TGACTACGAT 
GATGATTACA ACTATTTCGA TGAGGAGACG GTGACGATTT CCCCCAGTCC CAAGAAGGCC 
AAGAGCAACC CCAAGCTGCA GCCCATTGTG GATGCCACCA TCACGAAGTC CAAGACCAAA 
GAGAGTGCTA ACGATATCGA CAGCGACTCT CAATATGACT ACGACGAGGG TCTGGATGAT 
GATGATGACG AGGACGATGC CGCTGACGAC CTTTTGACCG ATGATGAGCA AGTTGTCTTC 
AGCGAGGACG TTCCGTGTCC AAGATTCTGC CAGTGTGCCC GGAATGTCAA CAGCTATCTG 
GTGGCCACCT GCAGCAGACT TGACATGGGC ATCCAGAAGT TTGGCTCTGA CATTACGGAT 
TTGGTTGTGA CCAATGTGGG TCCTAAGTAC CCCATCCTGA TGGGACCCAA CTTCTTCCAG 
AATCTGGGTC TTAAGAACGT GGCTTCCATC AAGATCGCCA ACTGCACCTT GGAGTACCTG 
CACGCCGAGG CCTTCCACGG ACTGAACGAG CTGTACGCTG TTAACTTGAC GGATGTTGGT 
TTGGCTATCA TTAATCCCGA TACCTTTGTT GGAAACAAGA AACTGCGCAT GCTGACCATT 
TCGGGTAACG ACTTGAGTGT GATGTCCAGC ATTCACTATT TGCTGAAGTC TTCCAGCATT 
GAGGAGCTGG ACTTCTCGCG CAACAACCTT ATGGAGTTGA ATCCCAAGGC CTTCTCCCAT 
CTATCCAATG TCGTCTACAT TAACTTGAGC CAGAACAGTC TCAAGAAACT GCCCGAGAAG 
GCTTTCGAGA AGGTTACTCT TCTGGAAGAA TTGGATCTGT CTTACAACTC GCTGACTGAA 
TTGCCCCGCG ATATCTTCAA TGGCACCACC CTGTCCATAC TGCACCTGAA GTACAATACC 
TTCAATGGCG ATCTGCACTT TGGCACCAAG GATCTGCAGC AGCTGGACTT GAGCTTTAAC 
TCGATTGTCC AAGTGCACCA CAGCATGTTC GACAAGATGC CCGGTCTGAC CAACCTGAAC 
CTCAAGGGCA ATGGCATTAA GAAGATCCAA CCGGACTCGT TCCTTACTTT GAAGAACCTG 
CGCCACATCG ATTTGTCCAT CAACGATCTG GACCAGATCT CGGGAATGCT GTTCTTTAAG 
AACTCTGAGC TGGATGTGAT CCGTTTGAAC GACAATCCTC GTCTGAGCCA GTTGCCCACC 
GATGGCTTCT TGAGCTACAG TGGGGAGTTC ACCGTCTACT ACCTGGATAT TTCCAATTGC 
GCCATCGGTC CATTGGGTCA CAAGGCCTTC TCCACTATGC CGCATTTGAC CACCTTGAAG 
TTGGCCTGGA ACAACATTAA TCATCTGCCG CGCGAGATTT TCACTGGACT GCACAAGCTG 
ATTGACTTGG ATCTGAGCAA CAACCTGATC ACCCGCATGG ACGATCTGAT CTTCATGGAC 
AACGGAGAGC TGACTAAGCT CAGTCTGGCT GGAAATCCCA TTAGCCGCTT GTCCGTCCGC 
CTCTTCCTGC CGCTGCACCA ACTGCGATGC CTGGACGTCA ATGACTGCGA GCTTACCACC 
TTGCTCTCCG ATCGCGACTT GGGTGCGGGT TACAAGATCT TTGACAGCCT GCGCTCCTTT 
AACGCCTCTG GTAATTTGAT CAAGAAGATC TCTTCGGAGG ATGTGAAGAG CTTCAAGAAC 
CTGCGTTCTC TGGATATCAC CAACAATCCG CTGAAGTGCA CTCCCGACTT CCAGGAGTTC 
ATCAGCTACG TCACTCTTCA GATGCAGATG ACTCCCAAGC GTCTGCCCGT GCTGGCCAAT 
CTGGAGGACG ATGCTACTAT TGTGCAGCTG GAGACTCTGG CTCAGGCTGG TTGGTCGTCT 
TTGGCCCACG AGGTGTGCAA GCATGCCGAA GGATCAGATC TCTTGGACGA AAAGAAGGCC 
GACAGTGCCG AAGCCAAACT GGAGAAGCGT CTGAAGGAAA GCGTGAAGAA GCTTAACGAG 
GATGAGGCTA AGTTGCTGAG TGTCCTGGAC AAGAGTGTGC TAGACAAGAG CAGGCTGAGC 
AGCATGCAGA AGATCATGAA GGGATCGGTG AACACTGAAA CCGCGAAGCC TGAGGAAGAT 
GCTGACGAGG AGAATCTGAA CAATGGCGGC GAGGATAAGG AGGATGACAG CAGTGACTAT 
GACAGTGAGG AGGATGATGA TGACGATGAT GAGGACGATG ATGATGACAA CAACGATGAT 
GATCGCTTCG AGGAGGCCAA GAAGAATGCC AAGGCTAAGG CCGATGCTAA CAAGGCCTTC 
GACACGTTCG TGAACAAGGA ACTGAAGCCA GTCCTGAAGA CCGATGGTAT CCAGGCGGAG 
GAAGTGGACA TGTCTCAGGA GACCCGTGAT AAATTCCTTC TGGAGAAACT GGGCTTCGAC 
AGCGAAAGCG AGGAGTCAGA TGAGTACACC GAGAAGATGA TCTTCCACGG CGAACTATCC 
TACGACCTGG TGGTCCCACT GATCGTCGTG TCCTTCTGCC TCCTGATGAT CGTGCTGTCC 
ATCGCCCGAG TGATCTACGT GGTGCTGCGC AAGCGCGGCG AGCGCTATCG CATGGCGCTG 
CTGGCCTCAA AGAACTCGTT CGTATACCAG AAGTTGAGCG AGGACATCGT GAAGCCGACC 
AGCAAGGAGC AGAAGGAGCC CAAGCATCTG AAGCAGCCAA AGGTGCACCG ATACGCGCCC 
ATCAATCAGG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Gp150 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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