HmgD cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	HmgD
Entrez Gene ID	37481
Full Name	High mobility group protein D
Synonyms	CG17950,Dm-HMG-D100,Dmel\CG17950,HMG D,HMG-D,HMG-D100,HMG1,HMGD,Hmg-D,anon-EST:Liang-1.9,clone 1.9,dHMG-D,hmg-D,hmgd
General protein information	Preferred Names High mobility group protein D Names CG17950 gene product from transcript CG17950-RD CG17950-PA CG17950-PB CG17950-PC CG17950-PD HmgD-PA HmgD-PB HmgD-PC HmgD-PD high mobility group protein D
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 57F9-57F10

mRNA and Protein(s)

mRNA	Protein	Name
NM_001299767.1	NP_001286696.1	high mobility group protein D, isoform D
NM_166479.2	NP_726109.1	high mobility group protein D, isoform A
NM_166480.2	NP_726110.1	high mobility group protein D, isoform B
NM_001169773.2	NP_001163244.1	high mobility group protein D, isoform C

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012334	XP_001238271.1
Drosophila melanogaster (fruit fly)	HmgZ	NP_726107.1
Drosophila melanogaster (fruit fly)	HmgD	NP_726109.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HMG-D and ZmHMGB1 exist in dynamic assemblies in which the basic HMG boxes and linkers associate with their intrinsically disordered, predominantly acidic, tails in a manner analogous to that observed previously for HMGB1.
Title: Structural insights into the mechanism of negative regulation of single-box high mobility group proteins by the acidic tail domain.

This study provides new insights into the binding mechanisms used by HMG boxes to recognize varied DNA structures and sequences as well as modulate DNA structure and DNA bending.
Title: Structural analysis of HMGD-DNA complexes reveals influence of intercalation on sequence selectivity and DNA bending.

A major function of the HMGD protein in Drosophila is to participate in Brahma-dependent chromatin remodeling at a specific subset of Brahma-dependent promoters.
Title: High mobility group proteins HMGD and HMGZ interact genetically with the Brahma chromatin remodeling complex in Drosophila.

DNA binding is entropy driven with a substantial non-electrostatic contribution
Title: DNA binding of a non-sequence-specific HMG-D protein is entropy driven with a substantial non-electrostatic contribution.

The following HmgD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HmgD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10847	NM_166479.2 Latest version!	Drosophila melanogaster high mobility group protein D, transcript variant A (HmgD), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa10847	NM_001169773.2 Latest version!	Drosophila melanogaster high mobility group protein D, transcript variant C (HmgD), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa10847	NM_166480.2 Latest version!	Drosophila melanogaster high mobility group protein D, transcript variant B (HmgD), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa10847	NM_001299767.1 Latest version!	Drosophila melanogaster high mobility group protein D, transcript variant D (HmgD), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HmgD ( NM_001299767.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286696.1 Drosophila melanogaster high mobility group protein D, transcript variant D (HmgD), mRNA.

Price & Availability↑

CloneID	OFa10847
Clone ID Related Accession (Same CDS sequence)	NM_001299767.1 , NM_166479.2 , NM_166480.2 , NM_001169773.2
Accession Version	NM_001299767.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	high mobility group protein D, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGATA AGCCAAAACG CCCACTCTCC GCCTACATGC TGTGGCTCAA CAGTGCCCGC 
GAGTCGATCA AGCGCGAGAA TCCCGGCATC AAGGTCACCG AGGTGGCCAA GCGCGGTGGT 
GAATTATGGA GGGCAATGAA GGACAAGTCT GAGTGGGAGG CCAAGGCTGC TAAGGCCAAG 
GACGACTACG ATCGGGCGGT CAAGGAGTTC GAGGCCAATG GTGGCAGCAG TGCTGCCAAC 
GGTGGTGGAG CCAAGAAGCG CGCGAAACCA GCGAAAAAGG TGGCGAAGAA GAGCAAGAAG 
GAGGAGTCCG ACGAGGACGA CGATGATGAG AGCGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286696.1
CDS	670..1008
Translation	MSDKPKRPLSAYMLWLNSARESIKRENPGIKVTEVAKRGGELWRAMKDKSEWEAKAAKAKDDYDRAVKEFEANGGSSAANGGGAKKRAKPAKKVAKKSKKEESDEDDDDESE

Target ORF information:

RefSeq Version	NM_001299767.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster high mobility group protein D, transcript variant D (HmgD), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299767.1

ORF Insert Sequence:

ATGTCTGATA AGCCAAAACG CCCACTCTCC GCCTACATGC TGTGGCTCAA CAGTGCCCGC 
GAGTCGATCA AGCGCGAGAA TCCCGGCATC AAGGTCACCG AGGTGGCCAA GCGCGGTGGT 
GAATTATGGA GGGCAATGAA GGACAAGTCT GAGTGGGAGG CCAAGGCTGC TAAGGCCAAG 
GACGACTACG ATCGGGCGGT CAAGGAGTTC GAGGCCAATG GTGGCAGCAG TGCTGCCAAC 
GGTGGTGGAG CCAAGAAGCG CGCGAAACCA GCGAAAAAGG TGGCGAAGAA GAGCAAGAAG 
GAGGAGTCCG ACGAGGACGA CGATGATGAG AGCGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HmgD ( NM_166479.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726109.1 Drosophila melanogaster high mobility group protein D, transcript variant A (HmgD), mRNA.

Price & Availability↑

CloneID	OFa10847
Clone ID Related Accession (Same CDS sequence)	NM_001299767.1 , NM_166479.2 , NM_166480.2 , NM_001169773.2
Accession Version	NM_166479.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	high mobility group protein D, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166479.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGATA AGCCAAAACG CCCACTCTCC GCCTACATGC TGTGGCTCAA CAGTGCCCGC 
GAGTCGATCA AGCGCGAGAA TCCCGGCATC AAGGTCACCG AGGTGGCCAA GCGCGGTGGT 
GAATTATGGA GGGCAATGAA GGACAAGTCT GAGTGGGAGG CCAAGGCTGC TAAGGCCAAG 
GACGACTACG ATCGGGCGGT CAAGGAGTTC GAGGCCAATG GTGGCAGCAG TGCTGCCAAC 
GGTGGTGGAG CCAAGAAGCG CGCGAAACCA GCGAAAAAGG TGGCGAAGAA GAGCAAGAAG 
GAGGAGTCCG ACGAGGACGA CGATGATGAG AGCGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726109.1
CDS	95..433
Translation	MSDKPKRPLSAYMLWLNSARESIKRENPGIKVTEVAKRGGELWRAMKDKSEWEAKAAKAKDDYDRAVKEFEANGGSSAANGGGAKKRAKPAKKVAKKSKKEESDEDDDDESE

Target ORF information:

RefSeq Version	NM_166479.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster high mobility group protein D, transcript variant A (HmgD), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166479.2

ORF Insert Sequence:

ATGTCTGATA AGCCAAAACG CCCACTCTCC GCCTACATGC TGTGGCTCAA CAGTGCCCGC 
GAGTCGATCA AGCGCGAGAA TCCCGGCATC AAGGTCACCG AGGTGGCCAA GCGCGGTGGT 
GAATTATGGA GGGCAATGAA GGACAAGTCT GAGTGGGAGG CCAAGGCTGC TAAGGCCAAG 
GACGACTACG ATCGGGCGGT CAAGGAGTTC GAGGCCAATG GTGGCAGCAG TGCTGCCAAC 
GGTGGTGGAG CCAAGAAGCG CGCGAAACCA GCGAAAAAGG TGGCGAAGAA GAGCAAGAAG 
GAGGAGTCCG ACGAGGACGA CGATGATGAG AGCGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HmgD ( NM_166480.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726110.1 Drosophila melanogaster high mobility group protein D, transcript variant B (HmgD), mRNA.

Price & Availability↑

CloneID	OFa10847
Clone ID Related Accession (Same CDS sequence)	NM_001299767.1 , NM_166479.2 , NM_166480.2 , NM_001169773.2
Accession Version	NM_166480.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	high mobility group protein D, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166480.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGATA AGCCAAAACG CCCACTCTCC GCCTACATGC TGTGGCTCAA CAGTGCCCGC 
GAGTCGATCA AGCGCGAGAA TCCCGGCATC AAGGTCACCG AGGTGGCCAA GCGCGGTGGT 
GAATTATGGA GGGCAATGAA GGACAAGTCT GAGTGGGAGG CCAAGGCTGC TAAGGCCAAG 
GACGACTACG ATCGGGCGGT CAAGGAGTTC GAGGCCAATG GTGGCAGCAG TGCTGCCAAC 
GGTGGTGGAG CCAAGAAGCG CGCGAAACCA GCGAAAAAGG TGGCGAAGAA GAGCAAGAAG 
GAGGAGTCCG ACGAGGACGA CGATGATGAG AGCGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726110.1
CDS	94..432
Translation	MSDKPKRPLSAYMLWLNSARESIKRENPGIKVTEVAKRGGELWRAMKDKSEWEAKAAKAKDDYDRAVKEFEANGGSSAANGGGAKKRAKPAKKVAKKSKKEESDEDDDDESE

Target ORF information:

RefSeq Version	NM_166480.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster high mobility group protein D, transcript variant B (HmgD), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166480.2

ORF Insert Sequence:

ATGTCTGATA AGCCAAAACG CCCACTCTCC GCCTACATGC TGTGGCTCAA CAGTGCCCGC 
GAGTCGATCA AGCGCGAGAA TCCCGGCATC AAGGTCACCG AGGTGGCCAA GCGCGGTGGT 
GAATTATGGA GGGCAATGAA GGACAAGTCT GAGTGGGAGG CCAAGGCTGC TAAGGCCAAG 
GACGACTACG ATCGGGCGGT CAAGGAGTTC GAGGCCAATG GTGGCAGCAG TGCTGCCAAC 
GGTGGTGGAG CCAAGAAGCG CGCGAAACCA GCGAAAAAGG TGGCGAAGAA GAGCAAGAAG 
GAGGAGTCCG ACGAGGACGA CGATGATGAG AGCGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HmgD ( NM_001169773.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163244.1 Drosophila melanogaster high mobility group protein D, transcript variant C (HmgD), mRNA.

Price & Availability↑

CloneID	OFa10847
Clone ID Related Accession (Same CDS sequence)	NM_001299767.1 , NM_166479.2 , NM_166480.2 , NM_001169773.2
Accession Version	NM_001169773.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	high mobility group protein D, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001169773.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGATA AGCCAAAACG CCCACTCTCC GCCTACATGC TGTGGCTCAA CAGTGCCCGC 
GAGTCGATCA AGCGCGAGAA TCCCGGCATC AAGGTCACCG AGGTGGCCAA GCGCGGTGGT 
GAATTATGGA GGGCAATGAA GGACAAGTCT GAGTGGGAGG CCAAGGCTGC TAAGGCCAAG 
GACGACTACG ATCGGGCGGT CAAGGAGTTC GAGGCCAATG GTGGCAGCAG TGCTGCCAAC 
GGTGGTGGAG CCAAGAAGCG CGCGAAACCA GCGAAAAAGG TGGCGAAGAA GAGCAAGAAG 
GAGGAGTCCG ACGAGGACGA CGATGATGAG AGCGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163244.1
CDS	142..480
Translation	MSDKPKRPLSAYMLWLNSARESIKRENPGIKVTEVAKRGGELWRAMKDKSEWEAKAAKAKDDYDRAVKEFEANGGSSAANGGGAKKRAKPAKKVAKKSKKEESDEDDDDESE

Target ORF information:

RefSeq Version	NM_001169773.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster high mobility group protein D, transcript variant C (HmgD), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169773.2

ORF Insert Sequence:

ATGTCTGATA AGCCAAAACG CCCACTCTCC GCCTACATGC TGTGGCTCAA CAGTGCCCGC 
GAGTCGATCA AGCGCGAGAA TCCCGGCATC AAGGTCACCG AGGTGGCCAA GCGCGGTGGT 
GAATTATGGA GGGCAATGAA GGACAAGTCT GAGTGGGAGG CCAAGGCTGC TAAGGCCAAG 
GACGACTACG ATCGGGCGGT CAAGGAGTTC GAGGCCAATG GTGGCAGCAG TGCTGCCAAC 
GGTGGTGGAG CCAAGAAGCG CGCGAAACCA GCGAAAAAGG TGGCGAAGAA GAGCAAGAAG 
GAGGAGTCCG ACGAGGACGA CGATGATGAG AGCGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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HmgD cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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