Lis-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lis-1
Entrez Gene ID	36791
Full Name	Lissencephaly-1
Synonyms	CG8440,D-Lis1,DLIS-1,DLIS1,DLis-1,DLis1,Dlis-1,Dlis1,DmLIS1,Dmel\CG8440,LIS-1,Lis 1,Lis1,dLis1,l(2)k13209,l(2R)W8,lis-1,lis1,n(2)k11702
General protein information	Preferred Names Lissencephaly-1 Names CG8440 gene product from transcript CG8440-RA CG8440-PA CG8440-PB CG8440-PF CG8440-PG Lis-1-PA Lis-1-PB Lis-1-PF Lis-1-PG Paf-AHbeta lissencephaly-1 platelet-activating factor acetylhydrolase Ib beta-subunit homolog
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 52F4-52F5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001259432.2	NP_001246361.1	Lissencephaly-1, isoform G
NM_057812.5	NP_477160.1	Lissencephaly-1, isoform A
NM_176190.2	NP_788370.1	Lissencephaly-1, isoform B
NM_206130.2	NP_995852.1	Lissencephaly-1, isoform F

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011849	XP_320670.3
Neurospora crassa	NCU04312	XP_960958.2
Danio rerio (zebrafish)	pafah1b1b	NP_958503.1
Drosophila melanogaster (fruit fly)	Lis-1	NP_995852.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

lowering levels of Lissencephaly-1 (Lis1), a dynein motor co-factor, augments axonal mitochondrial transport in ageing wing neurons.
Title: Reducing Lissencephaly-1 levels augments mitochondrial transport and has a protective effect in adult Drosophila neurons.

The data reveal a critical role for Lis1 within the mRNA localization machinery and suggest a model in which Lis1 facilitates motor complex association with cargos by promoting the interaction of dynein with dynactin.
Title: Lissencephaly-1 promotes the recruitment of dynein and dynactin to transported mRNAs.

present a model in which Lis-1 and asun cooperate to regulate dynein localization and centrosome positioning during spermatogenesis
Title: Regulation of dynein localization and centrosome positioning by Lis-1 and asunder during Drosophila spermatogenesis.

A general role of Lis-1/NudE, Dynein and microtubules, in cell-on-cell migration, is reported.
Title: Microtubules and Lis-1/NudE/dynein regulate invasive cell-on-cell migration in Drosophila.

Lis1, which is widely expressed in adult mammalian tissues, could regulate adult tissue stem cells through modulating niche signaling and adhesion.
Title: Lissencephaly-1 controls germline stem cell self-renewal through modulating bone morphogenetic protein signaling and niche adhesion.

The following Lis-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lis-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10178	NM_001259432.2 Latest version!	Drosophila melanogaster Lissencephaly-1 (Lis-1), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa10178	NM_057812.5 Latest version!	Drosophila melanogaster Lissencephaly-1 (Lis-1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa10178	NM_176190.2 Latest version!	Drosophila melanogaster Lissencephaly-1 (Lis-1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa10178	NM_206130.2 Latest version!	Drosophila melanogaster Lissencephaly-1 (Lis-1), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lis-1 ( NM_001259432.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246361.1 Drosophila melanogaster Lissencephaly-1 (Lis-1), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa10178
Clone ID Related Accession (Same CDS sequence)	NM_001259432.2 , NM_057812.5 , NM_176190.2 , NM_206130.2
Accession Version	NM_001259432.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Lissencephaly-1, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001259432.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAATGG TGTTGTCGCA GCGGCAGCGC GAGGAGCTTA ACCAAGCGAT TGCCGATTAT 
CTGGGCTCAA ATGGCTACGC CGACTCATTG GAGACCTTCC GCAAGGAGGC CGATCTCAGC 
ACAGAAGTGG AAAAGAAATT TGGCGGACTG TTGGAAAAGA AGTGGACGTC CGTGATTCGG 
CTGCAGAAGA AAGTGATGGA ACTAGAGGCT AAGCTCACTG AAGCTGAGAA GGAGGTCATC 
GAGGGGGCGC CCACAAAGAA CAAACGGACG CCTGGCGAGT GGATACCTCG GCCGCCAGAG 
AAGTTCTCCC TGACCGGTCA TCGTGCCAGC ATAACGAGGG TAATTTTCCA CCCCATCTTT 
GCTCTTATGG TTTCCGCATC CGAAGACGCC ACCATTAGGA TTTGGGACTT CGAAACTGGC 
GAGTACGAAC GCAGCCTGAA GGGACACACG GACTCTGTGC AGGATGTCGC ATTTGATGCC 
CAGGGCAAGC TTTTGGCCTC CTGCAGCGCG GACTTATCCA TCAAATTGTG GGATTTCCAG 
CAGAGCTACG AGTGTATCAA AACTATGCAT GGTCACGATC ACAATGTCTC GTCGGTGGCC 
TTTGTGCCGG CCGGAGATTA TGTACTCTCT GCATCCCGGG ACCGCACTAT TAAAATGTGG 
GAGGTGGCCA CAGGCTACTG TGTGAAAACC TACACAGGCC ATCGAGAGTG GGTGCGAATG 
GTGCGAGTGC ACATCGAAGG CAGCATCTTT GCCACATGCT CAAATGATCA GACAATACGT 
GTTTGGTTGA CGAATTCAAA AGACTGCAAG GTTGAATTAC GCGATCATGA GCATACTGTG 
GAGTGCATTG CCTGGGCGCC GGAAGCCGCA GCCTCTGCGA TAAACGAGGC AGCCGGAGCA 
GACAACAAGA AGGGCCATCA CCAGGGCCCG TTCCTGGCCT CCGGCTCTCG CGACAAAACC 
ATTCGCATTT GGGACGTGAG CGTCGGCCTG TGCCTGCTTA CCCTGAGTGG CCATGACAAT 
TGGGTGCGTG GCCTGGCATT CCATCCTGGT GGCAAATACT TGGTGTCGGC CAGCGATGAC 
AAGACCATTC GGGTGTGGGA CTTGCGCAAC AAGCGATGCA TGAAGACGCT ATACGCGCAT 
CAGCATTTCT GCACCTCCAT AGATTTTCAC AAAGCGCATC CGTACGTAAT TAGCGGTAGC 
GTAGATCAAA CAGTAAAGGT CTGGGAATGT CGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246361.1
CDS	125..1360
Translation	MKMVLSQRQREELNQAIADYLGSNGYADSLETFRKEADLSTEVEKKFGGLLEKKWTSVIRLQKKVMELEAKLTEAEKEVIEGAPTKNKRTPGEWIPRPPEKFSLTGHRASITRVIFHPIFALMVSASEDATIRIWDFETGEYERSLKGHTDSVQDVAFDAQGKLLASCSADLSIKLWDFQQSYECIKTMHGHDHNVSSVAFVPAGDYVLSASRDRTIKMWEVATGYCVKTYTGHREWVRMVRVHIEGSIFATCSNDQTIRVWLTNSKDCKVELRDHEHTVECIAWAPEAAASAINEAAGADNKKGHHQGPFLASGSRDKTIRIWDVSVGLCLLTLSGHDNWVRGLAFHPGGKYLVSASDDKTIRVWDLRNKRCMKTLYAHQHFCTSIDFHKAHPYVISGSVDQTVKVWECR

Target ORF information:

RefSeq Version	NM_001259432.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Lissencephaly-1 (Lis-1), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259432.2

ORF Insert Sequence:

ATGAAAATGG TGTTGTCGCA GCGGCAGCGC GAGGAGCTTA ACCAAGCGAT TGCCGATTAT 
CTGGGCTCAA ATGGCTACGC CGACTCATTG GAGACCTTCC GCAAGGAGGC CGATCTCAGC 
ACAGAAGTGG AAAAGAAATT TGGCGGACTG TTGGAAAAGA AGTGGACGTC CGTGATTCGG 
CTGCAGAAGA AAGTGATGGA ACTAGAGGCT AAGCTCACTG AAGCTGAGAA GGAGGTCATC 
GAGGGGGCGC CCACAAAGAA CAAACGGACG CCTGGCGAGT GGATACCTCG GCCGCCAGAG 
AAGTTCTCCC TGACCGGTCA TCGTGCCAGC ATAACGAGGG TAATTTTCCA CCCCATCTTT 
GCTCTTATGG TTTCCGCATC CGAAGACGCC ACCATTAGGA TTTGGGACTT CGAAACTGGC 
GAGTACGAAC GCAGCCTGAA GGGACACACG GACTCTGTGC AGGATGTCGC ATTTGATGCC 
CAGGGCAAGC TTTTGGCCTC CTGCAGCGCG GACTTATCCA TCAAATTGTG GGATTTCCAG 
CAGAGCTACG AGTGTATCAA AACTATGCAT GGTCACGATC ACAATGTCTC GTCGGTGGCC 
TTTGTGCCGG CCGGAGATTA TGTACTCTCT GCATCCCGGG ACCGCACTAT TAAAATGTGG 
GAGGTGGCCA CAGGCTACTG TGTGAAAACC TACACAGGCC ATCGAGAGTG GGTGCGAATG 
GTGCGAGTGC ACATCGAAGG CAGCATCTTT GCCACATGCT CAAATGATCA GACAATACGT 
GTTTGGTTGA CGAATTCAAA AGACTGCAAG GTTGAATTAC GCGATCATGA GCATACTGTG 
GAGTGCATTG CCTGGGCGCC GGAAGCCGCA GCCTCTGCGA TAAACGAGGC AGCCGGAGCA 
GACAACAAGA AGGGCCATCA CCAGGGCCCG TTCCTGGCCT CCGGCTCTCG CGACAAAACC 
ATTCGCATTT GGGACGTGAG CGTCGGCCTG TGCCTGCTTA CCCTGAGTGG CCATGACAAT 
TGGGTGCGTG GCCTGGCATT CCATCCTGGT GGCAAATACT TGGTGTCGGC CAGCGATGAC 
AAGACCATTC GGGTGTGGGA CTTGCGCAAC AAGCGATGCA TGAAGACGCT ATACGCGCAT 
CAGCATTTCT GCACCTCCAT AGATTTTCAC AAAGCGCATC CGTACGTAAT TAGCGGTAGC 
GTAGATCAAA CAGTAAAGGT CTGGGAATGT CGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lis-1 ( NM_057812.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477160.1 Drosophila melanogaster Lissencephaly-1 (Lis-1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa10178
Clone ID Related Accession (Same CDS sequence)	NM_001259432.2 , NM_057812.5 , NM_176190.2 , NM_206130.2
Accession Version	NM_057812.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Lissencephaly-1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_057812.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAATGG TGTTGTCGCA GCGGCAGCGC GAGGAGCTTA ACCAAGCGAT TGCCGATTAT 
CTGGGCTCAA ATGGCTACGC CGACTCATTG GAGACCTTCC GCAAGGAGGC CGATCTCAGC 
ACAGAAGTGG AAAAGAAATT TGGCGGACTG TTGGAAAAGA AGTGGACGTC CGTGATTCGG 
CTGCAGAAGA AAGTGATGGA ACTAGAGGCT AAGCTCACTG AAGCTGAGAA GGAGGTCATC 
GAGGGGGCGC CCACAAAGAA CAAACGGACG CCTGGCGAGT GGATACCTCG GCCGCCAGAG 
AAGTTCTCCC TGACCGGTCA TCGTGCCAGC ATAACGAGGG TAATTTTCCA CCCCATCTTT 
GCTCTTATGG TTTCCGCATC CGAAGACGCC ACCATTAGGA TTTGGGACTT CGAAACTGGC 
GAGTACGAAC GCAGCCTGAA GGGACACACG GACTCTGTGC AGGATGTCGC ATTTGATGCC 
CAGGGCAAGC TTTTGGCCTC CTGCAGCGCG GACTTATCCA TCAAATTGTG GGATTTCCAG 
CAGAGCTACG AGTGTATCAA AACTATGCAT GGTCACGATC ACAATGTCTC GTCGGTGGCC 
TTTGTGCCGG CCGGAGATTA TGTACTCTCT GCATCCCGGG ACCGCACTAT TAAAATGTGG 
GAGGTGGCCA CAGGCTACTG TGTGAAAACC TACACAGGCC ATCGAGAGTG GGTGCGAATG 
GTGCGAGTGC ACATCGAAGG CAGCATCTTT GCCACATGCT CAAATGATCA GACAATACGT 
GTTTGGTTGA CGAATTCAAA AGACTGCAAG GTTGAATTAC GCGATCATGA GCATACTGTG 
GAGTGCATTG CCTGGGCGCC GGAAGCCGCA GCCTCTGCGA TAAACGAGGC AGCCGGAGCA 
GACAACAAGA AGGGCCATCA CCAGGGCCCG TTCCTGGCCT CCGGCTCTCG CGACAAAACC 
ATTCGCATTT GGGACGTGAG CGTCGGCCTG TGCCTGCTTA CCCTGAGTGG CCATGACAAT 
TGGGTGCGTG GCCTGGCATT CCATCCTGGT GGCAAATACT TGGTGTCGGC CAGCGATGAC 
AAGACCATTC GGGTGTGGGA CTTGCGCAAC AAGCGATGCA TGAAGACGCT ATACGCGCAT 
CAGCATTTCT GCACCTCCAT AGATTTTCAC AAAGCGCATC CGTACGTAAT TAGCGGTAGC 
GTAGATCAAA CAGTAAAGGT CTGGGAATGT CGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477160.1
CDS	200..1435
Translation	MKMVLSQRQREELNQAIADYLGSNGYADSLETFRKEADLSTEVEKKFGGLLEKKWTSVIRLQKKVMELEAKLTEAEKEVIEGAPTKNKRTPGEWIPRPPEKFSLTGHRASITRVIFHPIFALMVSASEDATIRIWDFETGEYERSLKGHTDSVQDVAFDAQGKLLASCSADLSIKLWDFQQSYECIKTMHGHDHNVSSVAFVPAGDYVLSASRDRTIKMWEVATGYCVKTYTGHREWVRMVRVHIEGSIFATCSNDQTIRVWLTNSKDCKVELRDHEHTVECIAWAPEAAASAINEAAGADNKKGHHQGPFLASGSRDKTIRIWDVSVGLCLLTLSGHDNWVRGLAFHPGGKYLVSASDDKTIRVWDLRNKRCMKTLYAHQHFCTSIDFHKAHPYVISGSVDQTVKVWECR

Target ORF information:

RefSeq Version	NM_057812.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Lissencephaly-1 (Lis-1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057812.5

ORF Insert Sequence:

ATGAAAATGG TGTTGTCGCA GCGGCAGCGC GAGGAGCTTA ACCAAGCGAT TGCCGATTAT 
CTGGGCTCAA ATGGCTACGC CGACTCATTG GAGACCTTCC GCAAGGAGGC CGATCTCAGC 
ACAGAAGTGG AAAAGAAATT TGGCGGACTG TTGGAAAAGA AGTGGACGTC CGTGATTCGG 
CTGCAGAAGA AAGTGATGGA ACTAGAGGCT AAGCTCACTG AAGCTGAGAA GGAGGTCATC 
GAGGGGGCGC CCACAAAGAA CAAACGGACG CCTGGCGAGT GGATACCTCG GCCGCCAGAG 
AAGTTCTCCC TGACCGGTCA TCGTGCCAGC ATAACGAGGG TAATTTTCCA CCCCATCTTT 
GCTCTTATGG TTTCCGCATC CGAAGACGCC ACCATTAGGA TTTGGGACTT CGAAACTGGC 
GAGTACGAAC GCAGCCTGAA GGGACACACG GACTCTGTGC AGGATGTCGC ATTTGATGCC 
CAGGGCAAGC TTTTGGCCTC CTGCAGCGCG GACTTATCCA TCAAATTGTG GGATTTCCAG 
CAGAGCTACG AGTGTATCAA AACTATGCAT GGTCACGATC ACAATGTCTC GTCGGTGGCC 
TTTGTGCCGG CCGGAGATTA TGTACTCTCT GCATCCCGGG ACCGCACTAT TAAAATGTGG 
GAGGTGGCCA CAGGCTACTG TGTGAAAACC TACACAGGCC ATCGAGAGTG GGTGCGAATG 
GTGCGAGTGC ACATCGAAGG CAGCATCTTT GCCACATGCT CAAATGATCA GACAATACGT 
GTTTGGTTGA CGAATTCAAA AGACTGCAAG GTTGAATTAC GCGATCATGA GCATACTGTG 
GAGTGCATTG CCTGGGCGCC GGAAGCCGCA GCCTCTGCGA TAAACGAGGC AGCCGGAGCA 
GACAACAAGA AGGGCCATCA CCAGGGCCCG TTCCTGGCCT CCGGCTCTCG CGACAAAACC 
ATTCGCATTT GGGACGTGAG CGTCGGCCTG TGCCTGCTTA CCCTGAGTGG CCATGACAAT 
TGGGTGCGTG GCCTGGCATT CCATCCTGGT GGCAAATACT TGGTGTCGGC CAGCGATGAC 
AAGACCATTC GGGTGTGGGA CTTGCGCAAC AAGCGATGCA TGAAGACGCT ATACGCGCAT 
CAGCATTTCT GCACCTCCAT AGATTTTCAC AAAGCGCATC CGTACGTAAT TAGCGGTAGC 
GTAGATCAAA CAGTAAAGGT CTGGGAATGT CGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lis-1 ( NM_176190.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788370.1 Drosophila melanogaster Lissencephaly-1 (Lis-1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa10178
Clone ID Related Accession (Same CDS sequence)	NM_001259432.2 , NM_057812.5 , NM_176190.2 , NM_206130.2
Accession Version	NM_176190.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Lissencephaly-1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On May 8, 2012 this sequence version replaced NM_176190.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAATGG TGTTGTCGCA GCGGCAGCGC GAGGAGCTTA ACCAAGCGAT TGCCGATTAT 
CTGGGCTCAA ATGGCTACGC CGACTCATTG GAGACCTTCC GCAAGGAGGC CGATCTCAGC 
ACAGAAGTGG AAAAGAAATT TGGCGGACTG TTGGAAAAGA AGTGGACGTC CGTGATTCGG 
CTGCAGAAGA AAGTGATGGA ACTAGAGGCT AAGCTCACTG AAGCTGAGAA GGAGGTCATC 
GAGGGGGCGC CCACAAAGAA CAAACGGACG CCTGGCGAGT GGATACCTCG GCCGCCAGAG 
AAGTTCTCCC TGACCGGTCA TCGTGCCAGC ATAACGAGGG TAATTTTCCA CCCCATCTTT 
GCTCTTATGG TTTCCGCATC CGAAGACGCC ACCATTAGGA TTTGGGACTT CGAAACTGGC 
GAGTACGAAC GCAGCCTGAA GGGACACACG GACTCTGTGC AGGATGTCGC ATTTGATGCC 
CAGGGCAAGC TTTTGGCCTC CTGCAGCGCG GACTTATCCA TCAAATTGTG GGATTTCCAG 
CAGAGCTACG AGTGTATCAA AACTATGCAT GGTCACGATC ACAATGTCTC GTCGGTGGCC 
TTTGTGCCGG CCGGAGATTA TGTACTCTCT GCATCCCGGG ACCGCACTAT TAAAATGTGG 
GAGGTGGCCA CAGGCTACTG TGTGAAAACC TACACAGGCC ATCGAGAGTG GGTGCGAATG 
GTGCGAGTGC ACATCGAAGG CAGCATCTTT GCCACATGCT CAAATGATCA GACAATACGT 
GTTTGGTTGA CGAATTCAAA AGACTGCAAG GTTGAATTAC GCGATCATGA GCATACTGTG 
GAGTGCATTG CCTGGGCGCC GGAAGCCGCA GCCTCTGCGA TAAACGAGGC AGCCGGAGCA 
GACAACAAGA AGGGCCATCA CCAGGGCCCG TTCCTGGCCT CCGGCTCTCG CGACAAAACC 
ATTCGCATTT GGGACGTGAG CGTCGGCCTG TGCCTGCTTA CCCTGAGTGG CCATGACAAT 
TGGGTGCGTG GCCTGGCATT CCATCCTGGT GGCAAATACT TGGTGTCGGC CAGCGATGAC 
AAGACCATTC GGGTGTGGGA CTTGCGCAAC AAGCGATGCA TGAAGACGCT ATACGCGCAT 
CAGCATTTCT GCACCTCCAT AGATTTTCAC AAAGCGCATC CGTACGTAAT TAGCGGTAGC 
GTAGATCAAA CAGTAAAGGT CTGGGAATGT CGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788370.1
CDS	113..1348
Translation	MKMVLSQRQREELNQAIADYLGSNGYADSLETFRKEADLSTEVEKKFGGLLEKKWTSVIRLQKKVMELEAKLTEAEKEVIEGAPTKNKRTPGEWIPRPPEKFSLTGHRASITRVIFHPIFALMVSASEDATIRIWDFETGEYERSLKGHTDSVQDVAFDAQGKLLASCSADLSIKLWDFQQSYECIKTMHGHDHNVSSVAFVPAGDYVLSASRDRTIKMWEVATGYCVKTYTGHREWVRMVRVHIEGSIFATCSNDQTIRVWLTNSKDCKVELRDHEHTVECIAWAPEAAASAINEAAGADNKKGHHQGPFLASGSRDKTIRIWDVSVGLCLLTLSGHDNWVRGLAFHPGGKYLVSASDDKTIRVWDLRNKRCMKTLYAHQHFCTSIDFHKAHPYVISGSVDQTVKVWECR

Target ORF information:

RefSeq Version	NM_176190.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Lissencephaly-1 (Lis-1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176190.2

ORF Insert Sequence:

ATGAAAATGG TGTTGTCGCA GCGGCAGCGC GAGGAGCTTA ACCAAGCGAT TGCCGATTAT 
CTGGGCTCAA ATGGCTACGC CGACTCATTG GAGACCTTCC GCAAGGAGGC CGATCTCAGC 
ACAGAAGTGG AAAAGAAATT TGGCGGACTG TTGGAAAAGA AGTGGACGTC CGTGATTCGG 
CTGCAGAAGA AAGTGATGGA ACTAGAGGCT AAGCTCACTG AAGCTGAGAA GGAGGTCATC 
GAGGGGGCGC CCACAAAGAA CAAACGGACG CCTGGCGAGT GGATACCTCG GCCGCCAGAG 
AAGTTCTCCC TGACCGGTCA TCGTGCCAGC ATAACGAGGG TAATTTTCCA CCCCATCTTT 
GCTCTTATGG TTTCCGCATC CGAAGACGCC ACCATTAGGA TTTGGGACTT CGAAACTGGC 
GAGTACGAAC GCAGCCTGAA GGGACACACG GACTCTGTGC AGGATGTCGC ATTTGATGCC 
CAGGGCAAGC TTTTGGCCTC CTGCAGCGCG GACTTATCCA TCAAATTGTG GGATTTCCAG 
CAGAGCTACG AGTGTATCAA AACTATGCAT GGTCACGATC ACAATGTCTC GTCGGTGGCC 
TTTGTGCCGG CCGGAGATTA TGTACTCTCT GCATCCCGGG ACCGCACTAT TAAAATGTGG 
GAGGTGGCCA CAGGCTACTG TGTGAAAACC TACACAGGCC ATCGAGAGTG GGTGCGAATG 
GTGCGAGTGC ACATCGAAGG CAGCATCTTT GCCACATGCT CAAATGATCA GACAATACGT 
GTTTGGTTGA CGAATTCAAA AGACTGCAAG GTTGAATTAC GCGATCATGA GCATACTGTG 
GAGTGCATTG CCTGGGCGCC GGAAGCCGCA GCCTCTGCGA TAAACGAGGC AGCCGGAGCA 
GACAACAAGA AGGGCCATCA CCAGGGCCCG TTCCTGGCCT CCGGCTCTCG CGACAAAACC 
ATTCGCATTT GGGACGTGAG CGTCGGCCTG TGCCTGCTTA CCCTGAGTGG CCATGACAAT 
TGGGTGCGTG GCCTGGCATT CCATCCTGGT GGCAAATACT TGGTGTCGGC CAGCGATGAC 
AAGACCATTC GGGTGTGGGA CTTGCGCAAC AAGCGATGCA TGAAGACGCT ATACGCGCAT 
CAGCATTTCT GCACCTCCAT AGATTTTCAC AAAGCGCATC CGTACGTAAT TAGCGGTAGC 
GTAGATCAAA CAGTAAAGGT CTGGGAATGT CGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lis-1 ( NM_206130.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995852.1 Drosophila melanogaster Lissencephaly-1 (Lis-1), transcript variant F, mRNA.

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CloneID	OFa10178
Clone ID Related Accession (Same CDS sequence)	NM_001259432.2 , NM_057812.5 , NM_176190.2 , NM_206130.2
Accession Version	NM_206130.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Lissencephaly-1, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On May 8, 2012 this sequence version replaced NM_206130.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAATGG TGTTGTCGCA GCGGCAGCGC GAGGAGCTTA ACCAAGCGAT TGCCGATTAT 
CTGGGCTCAA ATGGCTACGC CGACTCATTG GAGACCTTCC GCAAGGAGGC CGATCTCAGC 
ACAGAAGTGG AAAAGAAATT TGGCGGACTG TTGGAAAAGA AGTGGACGTC CGTGATTCGG 
CTGCAGAAGA AAGTGATGGA ACTAGAGGCT AAGCTCACTG AAGCTGAGAA GGAGGTCATC 
GAGGGGGCGC CCACAAAGAA CAAACGGACG CCTGGCGAGT GGATACCTCG GCCGCCAGAG 
AAGTTCTCCC TGACCGGTCA TCGTGCCAGC ATAACGAGGG TAATTTTCCA CCCCATCTTT 
GCTCTTATGG TTTCCGCATC CGAAGACGCC ACCATTAGGA TTTGGGACTT CGAAACTGGC 
GAGTACGAAC GCAGCCTGAA GGGACACACG GACTCTGTGC AGGATGTCGC ATTTGATGCC 
CAGGGCAAGC TTTTGGCCTC CTGCAGCGCG GACTTATCCA TCAAATTGTG GGATTTCCAG 
CAGAGCTACG AGTGTATCAA AACTATGCAT GGTCACGATC ACAATGTCTC GTCGGTGGCC 
TTTGTGCCGG CCGGAGATTA TGTACTCTCT GCATCCCGGG ACCGCACTAT TAAAATGTGG 
GAGGTGGCCA CAGGCTACTG TGTGAAAACC TACACAGGCC ATCGAGAGTG GGTGCGAATG 
GTGCGAGTGC ACATCGAAGG CAGCATCTTT GCCACATGCT CAAATGATCA GACAATACGT 
GTTTGGTTGA CGAATTCAAA AGACTGCAAG GTTGAATTAC GCGATCATGA GCATACTGTG 
GAGTGCATTG CCTGGGCGCC GGAAGCCGCA GCCTCTGCGA TAAACGAGGC AGCCGGAGCA 
GACAACAAGA AGGGCCATCA CCAGGGCCCG TTCCTGGCCT CCGGCTCTCG CGACAAAACC 
ATTCGCATTT GGGACGTGAG CGTCGGCCTG TGCCTGCTTA CCCTGAGTGG CCATGACAAT 
TGGGTGCGTG GCCTGGCATT CCATCCTGGT GGCAAATACT TGGTGTCGGC CAGCGATGAC 
AAGACCATTC GGGTGTGGGA CTTGCGCAAC AAGCGATGCA TGAAGACGCT ATACGCGCAT 
CAGCATTTCT GCACCTCCAT AGATTTTCAC AAAGCGCATC CGTACGTAAT TAGCGGTAGC 
GTAGATCAAA CAGTAAAGGT CTGGGAATGT CGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995852.1
CDS	630..1865
Translation	MKMVLSQRQREELNQAIADYLGSNGYADSLETFRKEADLSTEVEKKFGGLLEKKWTSVIRLQKKVMELEAKLTEAEKEVIEGAPTKNKRTPGEWIPRPPEKFSLTGHRASITRVIFHPIFALMVSASEDATIRIWDFETGEYERSLKGHTDSVQDVAFDAQGKLLASCSADLSIKLWDFQQSYECIKTMHGHDHNVSSVAFVPAGDYVLSASRDRTIKMWEVATGYCVKTYTGHREWVRMVRVHIEGSIFATCSNDQTIRVWLTNSKDCKVELRDHEHTVECIAWAPEAAASAINEAAGADNKKGHHQGPFLASGSRDKTIRIWDVSVGLCLLTLSGHDNWVRGLAFHPGGKYLVSASDDKTIRVWDLRNKRCMKTLYAHQHFCTSIDFHKAHPYVISGSVDQTVKVWECR

Target ORF information:

RefSeq Version	NM_206130.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Lissencephaly-1 (Lis-1), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206130.2

ORF Insert Sequence:

ATGAAAATGG TGTTGTCGCA GCGGCAGCGC GAGGAGCTTA ACCAAGCGAT TGCCGATTAT 
CTGGGCTCAA ATGGCTACGC CGACTCATTG GAGACCTTCC GCAAGGAGGC CGATCTCAGC 
ACAGAAGTGG AAAAGAAATT TGGCGGACTG TTGGAAAAGA AGTGGACGTC CGTGATTCGG 
CTGCAGAAGA AAGTGATGGA ACTAGAGGCT AAGCTCACTG AAGCTGAGAA GGAGGTCATC 
GAGGGGGCGC CCACAAAGAA CAAACGGACG CCTGGCGAGT GGATACCTCG GCCGCCAGAG 
AAGTTCTCCC TGACCGGTCA TCGTGCCAGC ATAACGAGGG TAATTTTCCA CCCCATCTTT 
GCTCTTATGG TTTCCGCATC CGAAGACGCC ACCATTAGGA TTTGGGACTT CGAAACTGGC 
GAGTACGAAC GCAGCCTGAA GGGACACACG GACTCTGTGC AGGATGTCGC ATTTGATGCC 
CAGGGCAAGC TTTTGGCCTC CTGCAGCGCG GACTTATCCA TCAAATTGTG GGATTTCCAG 
CAGAGCTACG AGTGTATCAA AACTATGCAT GGTCACGATC ACAATGTCTC GTCGGTGGCC 
TTTGTGCCGG CCGGAGATTA TGTACTCTCT GCATCCCGGG ACCGCACTAT TAAAATGTGG 
GAGGTGGCCA CAGGCTACTG TGTGAAAACC TACACAGGCC ATCGAGAGTG GGTGCGAATG 
GTGCGAGTGC ACATCGAAGG CAGCATCTTT GCCACATGCT CAAATGATCA GACAATACGT 
GTTTGGTTGA CGAATTCAAA AGACTGCAAG GTTGAATTAC GCGATCATGA GCATACTGTG 
GAGTGCATTG CCTGGGCGCC GGAAGCCGCA GCCTCTGCGA TAAACGAGGC AGCCGGAGCA 
GACAACAAGA AGGGCCATCA CCAGGGCCCG TTCCTGGCCT CCGGCTCTCG CGACAAAACC 
ATTCGCATTT GGGACGTGAG CGTCGGCCTG TGCCTGCTTA CCCTGAGTGG CCATGACAAT 
TGGGTGCGTG GCCTGGCATT CCATCCTGGT GGCAAATACT TGGTGTCGGC CAGCGATGAC 
AAGACCATTC GGGTGTGGGA CTTGCGCAAC AAGCGATGCA TGAAGACGCT ATACGCGCAT 
CAGCATTTCT GCACCTCCAT AGATTTTCAC AAAGCGCATC CGTACGTAAT TAGCGGTAGC 
GTAGATCAAA CAGTAAAGGT CTGGGAATGT CGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Lis-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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