CG8547 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG8547
Entrez Gene ID	36586
Full Name	CG8547 gene product from transcript CG8547-RE
Synonyms	Dmel\CG8547
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 50E6-50E6

mRNA and Protein(s)

mRNA	Protein	Name
NM_001299486.1	NP_001286415.1	uncharacterized protein, isoform E
NM_137103.4	NP_610947.3	uncharacterized protein, isoform C
NM_166044.3	NP_725373.2	uncharacterized protein, isoform D

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG8547	NP_610947.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006184	XP_316249.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG8547 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG8547 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09956	NM_166044.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant D (CG8547), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa09956	NM_001299486.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant E (CG8547), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa09956	NM_137103.4 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG8547), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG8547 ( NM_001299486.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286415.1 Drosophila melanogaster uncharacterized protein, transcript variant E (CG8547), mRNA.

Price & Availability↑

CloneID	OFa09956
Clone ID Related Accession (Same CDS sequence)	NM_001299486.1 , NM_137103.4 , NM_166044.3
Accession Version	NM_001299486.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGTACT CGAGCAGTTC GGAAAATTAC TCATCCTCGT TGCGGGACAA GCCCTCGAAA 
TGGGGCAGGA ACGACAAGAA CTACGCCTAT AAGAAGTCAT CCTATCAATA CTCGAGTTCT 
GGCGACAACA ATGTCTCGTA TCAGGGCAAA TCCCCCGACC AGAACATAGA TCAATTGGAT 
GCCTTGTTGG AGGATCTGAA GCAGGAACGA CAGATTACCA ACAGGGAACG CGATCTGCTG 
CCCAGCAATG GAACCACTTA TAGGACACTT GAACGCACTG ATCCCTCGGG CACCGTAACG 
AGGACTACGC GAGTGGTCAA GACCAGCAAG CACTCGGGCA CCGGTGGCCA GCAGCCATTT 
ATTGATGATG TGGAGACCTT CAGTCCCACC GACTACAGCA CCCTGCAGTC CACGGCCAAG 
TACTCCAGCT TGAAGAGGGA CGAGTTCCAG ACCAAGGACA AGCCCTATAC TCTGGCCCAC 
TCCCCCAGTG GTGGACACTA CGAGAGCAAG TCGAAGATCT ACGAAAGCAA TCGCACCATT 
AACAACAATG TGGAGCTGCT GCCTGCAACG AGCAGCACAC ATCAGACTTT GACCAGCGGC 
ACCACCATTG ACAAGGAGCT GCAGGACATC GCTCTGGGCG ATGGCATCCT GCCTGCCCCA 
GGCACCAAGG TGACCACCAC GGTGAGGACC TACACCTATG AGATCCCGGC CACTGGTCCC 
GGCAGCAACA CCAGCACCAT TAACCGCACC CACACGCTGA ACAACTCGAA TACGCTGAGC 
AGCACCTACA AGCACCACGA AAGCCACAAC TCCAGTCAGA ACTTCTCGCA GCTGTCGCCC 
GTTCCGCACC CACAGCCTAG CCATGTGCCA CAGACGGTGG TTTACAACAC TGAGAGCTAC 
TCCACGTTGA ACCGGAACAG CGATCGTCCG GTGGTTAGCA ACCAGTCGTA CGAGATCAGA 
GAGCACAAGG AGACCACCAC TCGCGGCGTG AGCCCACAGC CCCGTCAACT ACCTTTGGGA 
TCGGGTCCGG CGGGAACTCC ACCGGGCAAT CGTACGGTCA TCTACAACAT CCACAAGACG 
GATCACGTCA ACACAGTCAA CGAGCTGCCA CCACAGCAGC GCTATCCGCC GCGCAGTCCG 
GCTCACAGTC CCAACTACGG AGGACCACAC AGTCCACCAC TACAGCCGGG AGTGAATCGC 
TACTACTACA AGGAGACGGA GACCTCGAAC ACGGTCAATA GCATCCACGG ACCGCCGAAC 
GGTGGACCTT ACGAGCCGGG TCTACCTCAG TCCGCTCCGC TGAAACAGCT GCCGCCCAAC 
AACGCGTATC CACAGCAGCC ACCAAATGGA AATGGTTATC CACCGAATTC GACCACTTAC 
AGCTACAAAT ACTCCTCGAC CACCAACACA AACAACCGAC GTGGCCCGGA CTACGGCTCG 
CCATCTCCAT TCCCCGTGGA TGGCGTGGAG TATCCAGTGA ATAGCAATCC TCCCCAGCGA 
GTGGACGATC TGATGCAGTC ATTCGGAACT ACCACCGACC ATGTGGATCG CGTGGACATT 
GAGACTCCAG TGCGCAAGCG TGAAATCGAG ACGGCGGTTG CCTCGCCCAA CCAACAGCAT 
GTGCCATCGG TGAACAAGGC GGGAAAAGAG GTGTACTATC CACCCGGACA CGACACCATC 
GTCATGAAGC GCGAGGAGAT GCATGCTGGA TCTGCCTCAG GCGGTCGCTG GGCCAAGGGA 
TCCGGAATGT ACGAGTACGA GTCCGGCTCG AAGTCAAAGA CGAAAACCAA GTCCGGTGGC 
GCCGTTGTGC CCGTGTGCCT GCCCCTCTGC TGTGCCATGC CCTGCTCCAT TATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286415.1
CDS	204..2060
Translation	MQYSSSSENYSSSLRDKPSKWGRNDKNYAYKKSSYQYSSSGDNNVSYQGKSPDQNIDQLDALLEDLKQERQITNRERDLLPSNGTTYRTLERTDPSGTVTRTTRVVKTSKHSGTGGQQPFIDDVETFSPTDYSTLQSTAKYSSLKRDEFQTKDKPYTLAHSPSGGHYESKSKIYESNRTINNNVELLPATSSTHQTLTSGTTIDKELQDIALGDGILPAPGTKVTTTVRTYTYEIPATGPGSNTSTINRTHTLNNSNTLSSTYKHHESHNSSQNFSQLSPVPHPQPSHVPQTVVYNTESYSTLNRNSDRPVVSNQSYEIREHKETTTRGVSPQPRQLPLGSGPAGTPPGNRTVIYNIHKTDHVNTVNELPPQQRYPPRSPAHSPNYGGPHSPPLQPGVNRYYYKETETSNTVNSIHGPPNGGPYEPGLPQSAPLKQLPPNNAYPQQPPNGNGYPPNSTTYSYKYSSTTNTNNRRGPDYGSPSPFPVDGVEYPVNSNPPQRVDDLMQSFGTTTDHVDRVDIETPVRKREIETAVASPNQQHVPSVNKAGKEVYYPPGHDTIVMKREEMHAGSASGGRWAKGSGMYEYESGSKSKTKTKSGGAVVPVCLPLCCAMPCSIM

Target ORF information:

RefSeq Version	NM_001299486.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant E (CG8547), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299486.1

ORF Insert Sequence:

ATGCAGTACT CGAGCAGTTC GGAAAATTAC TCATCCTCGT TGCGGGACAA GCCCTCGAAA 
TGGGGCAGGA ACGACAAGAA CTACGCCTAT AAGAAGTCAT CCTATCAATA CTCGAGTTCT 
GGCGACAACA ATGTCTCGTA TCAGGGCAAA TCCCCCGACC AGAACATAGA TCAATTGGAT 
GCCTTGTTGG AGGATCTGAA GCAGGAACGA CAGATTACCA ACAGGGAACG CGATCTGCTG 
CCCAGCAATG GAACCACTTA TAGGACACTT GAACGCACTG ATCCCTCGGG CACCGTAACG 
AGGACTACGC GAGTGGTCAA GACCAGCAAG CACTCGGGCA CCGGTGGCCA GCAGCCATTT 
ATTGATGATG TGGAGACCTT CAGTCCCACC GACTACAGCA CCCTGCAGTC CACGGCCAAG 
TACTCCAGCT TGAAGAGGGA CGAGTTCCAG ACCAAGGACA AGCCCTATAC TCTGGCCCAC 
TCCCCCAGTG GTGGACACTA CGAGAGCAAG TCGAAGATCT ACGAAAGCAA TCGCACCATT 
AACAACAATG TGGAGCTGCT GCCTGCAACG AGCAGCACAC ATCAGACTTT GACCAGCGGC 
ACCACCATTG ACAAGGAGCT GCAGGACATC GCTCTGGGCG ATGGCATCCT GCCTGCCCCA 
GGCACCAAGG TGACCACCAC GGTGAGGACC TACACCTATG AGATCCCGGC CACTGGTCCC 
GGCAGCAACA CCAGCACCAT TAACCGCACC CACACGCTGA ACAACTCGAA TACGCTGAGC 
AGCACCTACA AGCACCACGA AAGCCACAAC TCCAGTCAGA ACTTCTCGCA GCTGTCGCCC 
GTTCCGCACC CACAGCCTAG CCATGTGCCA CAGACGGTGG TTTACAACAC TGAGAGCTAC 
TCCACGTTGA ACCGGAACAG CGATCGTCCG GTGGTTAGCA ACCAGTCGTA CGAGATCAGA 
GAGCACAAGG AGACCACCAC TCGCGGCGTG AGCCCACAGC CCCGTCAACT ACCTTTGGGA 
TCGGGTCCGG CGGGAACTCC ACCGGGCAAT CGTACGGTCA TCTACAACAT CCACAAGACG 
GATCACGTCA ACACAGTCAA CGAGCTGCCA CCACAGCAGC GCTATCCGCC GCGCAGTCCG 
GCTCACAGTC CCAACTACGG AGGACCACAC AGTCCACCAC TACAGCCGGG AGTGAATCGC 
TACTACTACA AGGAGACGGA GACCTCGAAC ACGGTCAATA GCATCCACGG ACCGCCGAAC 
GGTGGACCTT ACGAGCCGGG TCTACCTCAG TCCGCTCCGC TGAAACAGCT GCCGCCCAAC 
AACGCGTATC CACAGCAGCC ACCAAATGGA AATGGTTATC CACCGAATTC GACCACTTAC 
AGCTACAAAT ACTCCTCGAC CACCAACACA AACAACCGAC GTGGCCCGGA CTACGGCTCG 
CCATCTCCAT TCCCCGTGGA TGGCGTGGAG TATCCAGTGA ATAGCAATCC TCCCCAGCGA 
GTGGACGATC TGATGCAGTC ATTCGGAACT ACCACCGACC ATGTGGATCG CGTGGACATT 
GAGACTCCAG TGCGCAAGCG TGAAATCGAG ACGGCGGTTG CCTCGCCCAA CCAACAGCAT 
GTGCCATCGG TGAACAAGGC GGGAAAAGAG GTGTACTATC CACCCGGACA CGACACCATC 
GTCATGAAGC GCGAGGAGAT GCATGCTGGA TCTGCCTCAG GCGGTCGCTG GGCCAAGGGA 
TCCGGAATGT ACGAGTACGA GTCCGGCTCG AAGTCAAAGA CGAAAACCAA GTCCGGTGGC 
GCCGTTGTGC CCGTGTGCCT GCCCCTCTGC TGTGCCATGC CCTGCTCCAT TATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG8547 ( NM_137103.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610947.3 Drosophila melanogaster uncharacterized protein, transcript variant C (CG8547), mRNA.

Price & Availability↑

CloneID	OFa09956
Clone ID Related Accession (Same CDS sequence)	NM_001299486.1 , NM_137103.4 , NM_166044.3
Accession Version	NM_137103.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_137103.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGTACT CGAGCAGTTC GGAAAATTAC TCATCCTCGT TGCGGGACAA GCCCTCGAAA 
TGGGGCAGGA ACGACAAGAA CTACGCCTAT AAGAAGTCAT CCTATCAATA CTCGAGTTCT 
GGCGACAACA ATGTCTCGTA TCAGGGCAAA TCCCCCGACC AGAACATAGA TCAATTGGAT 
GCCTTGTTGG AGGATCTGAA GCAGGAACGA CAGATTACCA ACAGGGAACG CGATCTGCTG 
CCCAGCAATG GAACCACTTA TAGGACACTT GAACGCACTG ATCCCTCGGG CACCGTAACG 
AGGACTACGC GAGTGGTCAA GACCAGCAAG CACTCGGGCA CCGGTGGCCA GCAGCCATTT 
ATTGATGATG TGGAGACCTT CAGTCCCACC GACTACAGCA CCCTGCAGTC CACGGCCAAG 
TACTCCAGCT TGAAGAGGGA CGAGTTCCAG ACCAAGGACA AGCCCTATAC TCTGGCCCAC 
TCCCCCAGTG GTGGACACTA CGAGAGCAAG TCGAAGATCT ACGAAAGCAA TCGCACCATT 
AACAACAATG TGGAGCTGCT GCCTGCAACG AGCAGCACAC ATCAGACTTT GACCAGCGGC 
ACCACCATTG ACAAGGAGCT GCAGGACATC GCTCTGGGCG ATGGCATCCT GCCTGCCCCA 
GGCACCAAGG TGACCACCAC GGTGAGGACC TACACCTATG AGATCCCGGC CACTGGTCCC 
GGCAGCAACA CCAGCACCAT TAACCGCACC CACACGCTGA ACAACTCGAA TACGCTGAGC 
AGCACCTACA AGCACCACGA AAGCCACAAC TCCAGTCAGA ACTTCTCGCA GCTGTCGCCC 
GTTCCGCACC CACAGCCTAG CCATGTGCCA CAGACGGTGG TTTACAACAC TGAGAGCTAC 
TCCACGTTGA ACCGGAACAG CGATCGTCCG GTGGTTAGCA ACCAGTCGTA CGAGATCAGA 
GAGCACAAGG AGACCACCAC TCGCGGCGTG AGCCCACAGC CCCGTCAACT ACCTTTGGGA 
TCGGGTCCGG CGGGAACTCC ACCGGGCAAT CGTACGGTCA TCTACAACAT CCACAAGACG 
GATCACGTCA ACACAGTCAA CGAGCTGCCA CCACAGCAGC GCTATCCGCC GCGCAGTCCG 
GCTCACAGTC CCAACTACGG AGGACCACAC AGTCCACCAC TACAGCCGGG AGTGAATCGC 
TACTACTACA AGGAGACGGA GACCTCGAAC ACGGTCAATA GCATCCACGG ACCGCCGAAC 
GGTGGACCTT ACGAGCCGGG TCTACCTCAG TCCGCTCCGC TGAAACAGCT GCCGCCCAAC 
AACGCGTATC CACAGCAGCC ACCAAATGGA AATGGTTATC CACCGAATTC GACCACTTAC 
AGCTACAAAT ACTCCTCGAC CACCAACACA AACAACCGAC GTGGCCCGGA CTACGGCTCG 
CCATCTCCAT TCCCCGTGGA TGGCGTGGAG TATCCAGTGA ATAGCAATCC TCCCCAGCGA 
GTGGACGATC TGATGCAGTC ATTCGGAACT ACCACCGACC ATGTGGATCG CGTGGACATT 
GAGACTCCAG TGCGCAAGCG TGAAATCGAG ACGGCGGTTG CCTCGCCCAA CCAACAGCAT 
GTGCCATCGG TGAACAAGGC GGGAAAAGAG GTGTACTATC CACCCGGACA CGACACCATC 
GTCATGAAGC GCGAGGAGAT GCATGCTGGA TCTGCCTCAG GCGGTCGCTG GGCCAAGGGA 
TCCGGAATGT ACGAGTACGA GTCCGGCTCG AAGTCAAAGA CGAAAACCAA GTCCGGTGGC 
GCCGTTGTGC CCGTGTGCCT GCCCCTCTGC TGTGCCATGC CCTGCTCCAT TATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610947.3
CDS	240..2096
Translation	MQYSSSSENYSSSLRDKPSKWGRNDKNYAYKKSSYQYSSSGDNNVSYQGKSPDQNIDQLDALLEDLKQERQITNRERDLLPSNGTTYRTLERTDPSGTVTRTTRVVKTSKHSGTGGQQPFIDDVETFSPTDYSTLQSTAKYSSLKRDEFQTKDKPYTLAHSPSGGHYESKSKIYESNRTINNNVELLPATSSTHQTLTSGTTIDKELQDIALGDGILPAPGTKVTTTVRTYTYEIPATGPGSNTSTINRTHTLNNSNTLSSTYKHHESHNSSQNFSQLSPVPHPQPSHVPQTVVYNTESYSTLNRNSDRPVVSNQSYEIREHKETTTRGVSPQPRQLPLGSGPAGTPPGNRTVIYNIHKTDHVNTVNELPPQQRYPPRSPAHSPNYGGPHSPPLQPGVNRYYYKETETSNTVNSIHGPPNGGPYEPGLPQSAPLKQLPPNNAYPQQPPNGNGYPPNSTTYSYKYSSTTNTNNRRGPDYGSPSPFPVDGVEYPVNSNPPQRVDDLMQSFGTTTDHVDRVDIETPVRKREIETAVASPNQQHVPSVNKAGKEVYYPPGHDTIVMKREEMHAGSASGGRWAKGSGMYEYESGSKSKTKTKSGGAVVPVCLPLCCAMPCSIM

Target ORF information:

RefSeq Version	NM_137103.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG8547), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_137103.4

ORF Insert Sequence:

ATGCAGTACT CGAGCAGTTC GGAAAATTAC TCATCCTCGT TGCGGGACAA GCCCTCGAAA 
TGGGGCAGGA ACGACAAGAA CTACGCCTAT AAGAAGTCAT CCTATCAATA CTCGAGTTCT 
GGCGACAACA ATGTCTCGTA TCAGGGCAAA TCCCCCGACC AGAACATAGA TCAATTGGAT 
GCCTTGTTGG AGGATCTGAA GCAGGAACGA CAGATTACCA ACAGGGAACG CGATCTGCTG 
CCCAGCAATG GAACCACTTA TAGGACACTT GAACGCACTG ATCCCTCGGG CACCGTAACG 
AGGACTACGC GAGTGGTCAA GACCAGCAAG CACTCGGGCA CCGGTGGCCA GCAGCCATTT 
ATTGATGATG TGGAGACCTT CAGTCCCACC GACTACAGCA CCCTGCAGTC CACGGCCAAG 
TACTCCAGCT TGAAGAGGGA CGAGTTCCAG ACCAAGGACA AGCCCTATAC TCTGGCCCAC 
TCCCCCAGTG GTGGACACTA CGAGAGCAAG TCGAAGATCT ACGAAAGCAA TCGCACCATT 
AACAACAATG TGGAGCTGCT GCCTGCAACG AGCAGCACAC ATCAGACTTT GACCAGCGGC 
ACCACCATTG ACAAGGAGCT GCAGGACATC GCTCTGGGCG ATGGCATCCT GCCTGCCCCA 
GGCACCAAGG TGACCACCAC GGTGAGGACC TACACCTATG AGATCCCGGC CACTGGTCCC 
GGCAGCAACA CCAGCACCAT TAACCGCACC CACACGCTGA ACAACTCGAA TACGCTGAGC 
AGCACCTACA AGCACCACGA AAGCCACAAC TCCAGTCAGA ACTTCTCGCA GCTGTCGCCC 
GTTCCGCACC CACAGCCTAG CCATGTGCCA CAGACGGTGG TTTACAACAC TGAGAGCTAC 
TCCACGTTGA ACCGGAACAG CGATCGTCCG GTGGTTAGCA ACCAGTCGTA CGAGATCAGA 
GAGCACAAGG AGACCACCAC TCGCGGCGTG AGCCCACAGC CCCGTCAACT ACCTTTGGGA 
TCGGGTCCGG CGGGAACTCC ACCGGGCAAT CGTACGGTCA TCTACAACAT CCACAAGACG 
GATCACGTCA ACACAGTCAA CGAGCTGCCA CCACAGCAGC GCTATCCGCC GCGCAGTCCG 
GCTCACAGTC CCAACTACGG AGGACCACAC AGTCCACCAC TACAGCCGGG AGTGAATCGC 
TACTACTACA AGGAGACGGA GACCTCGAAC ACGGTCAATA GCATCCACGG ACCGCCGAAC 
GGTGGACCTT ACGAGCCGGG TCTACCTCAG TCCGCTCCGC TGAAACAGCT GCCGCCCAAC 
AACGCGTATC CACAGCAGCC ACCAAATGGA AATGGTTATC CACCGAATTC GACCACTTAC 
AGCTACAAAT ACTCCTCGAC CACCAACACA AACAACCGAC GTGGCCCGGA CTACGGCTCG 
CCATCTCCAT TCCCCGTGGA TGGCGTGGAG TATCCAGTGA ATAGCAATCC TCCCCAGCGA 
GTGGACGATC TGATGCAGTC ATTCGGAACT ACCACCGACC ATGTGGATCG CGTGGACATT 
GAGACTCCAG TGCGCAAGCG TGAAATCGAG ACGGCGGTTG CCTCGCCCAA CCAACAGCAT 
GTGCCATCGG TGAACAAGGC GGGAAAAGAG GTGTACTATC CACCCGGACA CGACACCATC 
GTCATGAAGC GCGAGGAGAT GCATGCTGGA TCTGCCTCAG GCGGTCGCTG GGCCAAGGGA 
TCCGGAATGT ACGAGTACGA GTCCGGCTCG AAGTCAAAGA CGAAAACCAA GTCCGGTGGC 
GCCGTTGTGC CCGTGTGCCT GCCCCTCTGC TGTGCCATGC CCTGCTCCAT TATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG8547 ( NM_166044.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725373.2 Drosophila melanogaster uncharacterized protein, transcript variant D (CG8547), mRNA.

Price & Availability↑

CloneID	OFa09956
Clone ID Related Accession (Same CDS sequence)	NM_001299486.1 , NM_137103.4 , NM_166044.3
Accession Version	NM_166044.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166044.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGTACT CGAGCAGTTC GGAAAATTAC TCATCCTCGT TGCGGGACAA GCCCTCGAAA 
TGGGGCAGGA ACGACAAGAA CTACGCCTAT AAGAAGTCAT CCTATCAATA CTCGAGTTCT 
GGCGACAACA ATGTCTCGTA TCAGGGCAAA TCCCCCGACC AGAACATAGA TCAATTGGAT 
GCCTTGTTGG AGGATCTGAA GCAGGAACGA CAGATTACCA ACAGGGAACG CGATCTGCTG 
CCCAGCAATG GAACCACTTA TAGGACACTT GAACGCACTG ATCCCTCGGG CACCGTAACG 
AGGACTACGC GAGTGGTCAA GACCAGCAAG CACTCGGGCA CCGGTGGCCA GCAGCCATTT 
ATTGATGATG TGGAGACCTT CAGTCCCACC GACTACAGCA CCCTGCAGTC CACGGCCAAG 
TACTCCAGCT TGAAGAGGGA CGAGTTCCAG ACCAAGGACA AGCCCTATAC TCTGGCCCAC 
TCCCCCAGTG GTGGACACTA CGAGAGCAAG TCGAAGATCT ACGAAAGCAA TCGCACCATT 
AACAACAATG TGGAGCTGCT GCCTGCAACG AGCAGCACAC ATCAGACTTT GACCAGCGGC 
ACCACCATTG ACAAGGAGCT GCAGGACATC GCTCTGGGCG ATGGCATCCT GCCTGCCCCA 
GGCACCAAGG TGACCACCAC GGTGAGGACC TACACCTATG AGATCCCGGC CACTGGTCCC 
GGCAGCAACA CCAGCACCAT TAACCGCACC CACACGCTGA ACAACTCGAA TACGCTGAGC 
AGCACCTACA AGCACCACGA AAGCCACAAC TCCAGTCAGA ACTTCTCGCA GCTGTCGCCC 
GTTCCGCACC CACAGCCTAG CCATGTGCCA CAGACGGTGG TTTACAACAC TGAGAGCTAC 
TCCACGTTGA ACCGGAACAG CGATCGTCCG GTGGTTAGCA ACCAGTCGTA CGAGATCAGA 
GAGCACAAGG AGACCACCAC TCGCGGCGTG AGCCCACAGC CCCGTCAACT ACCTTTGGGA 
TCGGGTCCGG CGGGAACTCC ACCGGGCAAT CGTACGGTCA TCTACAACAT CCACAAGACG 
GATCACGTCA ACACAGTCAA CGAGCTGCCA CCACAGCAGC GCTATCCGCC GCGCAGTCCG 
GCTCACAGTC CCAACTACGG AGGACCACAC AGTCCACCAC TACAGCCGGG AGTGAATCGC 
TACTACTACA AGGAGACGGA GACCTCGAAC ACGGTCAATA GCATCCACGG ACCGCCGAAC 
GGTGGACCTT ACGAGCCGGG TCTACCTCAG TCCGCTCCGC TGAAACAGCT GCCGCCCAAC 
AACGCGTATC CACAGCAGCC ACCAAATGGA AATGGTTATC CACCGAATTC GACCACTTAC 
AGCTACAAAT ACTCCTCGAC CACCAACACA AACAACCGAC GTGGCCCGGA CTACGGCTCG 
CCATCTCCAT TCCCCGTGGA TGGCGTGGAG TATCCAGTGA ATAGCAATCC TCCCCAGCGA 
GTGGACGATC TGATGCAGTC ATTCGGAACT ACCACCGACC ATGTGGATCG CGTGGACATT 
GAGACTCCAG TGCGCAAGCG TGAAATCGAG ACGGCGGTTG CCTCGCCCAA CCAACAGCAT 
GTGCCATCGG TGAACAAGGC GGGAAAAGAG GTGTACTATC CACCCGGACA CGACACCATC 
GTCATGAAGC GCGAGGAGAT GCATGCTGGA TCTGCCTCAG GCGGTCGCTG GGCCAAGGGA 
TCCGGAATGT ACGAGTACGA GTCCGGCTCG AAGTCAAAGA CGAAAACCAA GTCCGGTGGC 
GCCGTTGTGC CCGTGTGCCT GCCCCTCTGC TGTGCCATGC CCTGCTCCAT TATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725373.2
CDS	178..2034
Translation	MQYSSSSENYSSSLRDKPSKWGRNDKNYAYKKSSYQYSSSGDNNVSYQGKSPDQNIDQLDALLEDLKQERQITNRERDLLPSNGTTYRTLERTDPSGTVTRTTRVVKTSKHSGTGGQQPFIDDVETFSPTDYSTLQSTAKYSSLKRDEFQTKDKPYTLAHSPSGGHYESKSKIYESNRTINNNVELLPATSSTHQTLTSGTTIDKELQDIALGDGILPAPGTKVTTTVRTYTYEIPATGPGSNTSTINRTHTLNNSNTLSSTYKHHESHNSSQNFSQLSPVPHPQPSHVPQTVVYNTESYSTLNRNSDRPVVSNQSYEIREHKETTTRGVSPQPRQLPLGSGPAGTPPGNRTVIYNIHKTDHVNTVNELPPQQRYPPRSPAHSPNYGGPHSPPLQPGVNRYYYKETETSNTVNSIHGPPNGGPYEPGLPQSAPLKQLPPNNAYPQQPPNGNGYPPNSTTYSYKYSSTTNTNNRRGPDYGSPSPFPVDGVEYPVNSNPPQRVDDLMQSFGTTTDHVDRVDIETPVRKREIETAVASPNQQHVPSVNKAGKEVYYPPGHDTIVMKREEMHAGSASGGRWAKGSGMYEYESGSKSKTKTKSGGAVVPVCLPLCCAMPCSIM

Target ORF information:

RefSeq Version	NM_166044.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant D (CG8547), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166044.3

ORF Insert Sequence:

ATGCAGTACT CGAGCAGTTC GGAAAATTAC TCATCCTCGT TGCGGGACAA GCCCTCGAAA 
TGGGGCAGGA ACGACAAGAA CTACGCCTAT AAGAAGTCAT CCTATCAATA CTCGAGTTCT 
GGCGACAACA ATGTCTCGTA TCAGGGCAAA TCCCCCGACC AGAACATAGA TCAATTGGAT 
GCCTTGTTGG AGGATCTGAA GCAGGAACGA CAGATTACCA ACAGGGAACG CGATCTGCTG 
CCCAGCAATG GAACCACTTA TAGGACACTT GAACGCACTG ATCCCTCGGG CACCGTAACG 
AGGACTACGC GAGTGGTCAA GACCAGCAAG CACTCGGGCA CCGGTGGCCA GCAGCCATTT 
ATTGATGATG TGGAGACCTT CAGTCCCACC GACTACAGCA CCCTGCAGTC CACGGCCAAG 
TACTCCAGCT TGAAGAGGGA CGAGTTCCAG ACCAAGGACA AGCCCTATAC TCTGGCCCAC 
TCCCCCAGTG GTGGACACTA CGAGAGCAAG TCGAAGATCT ACGAAAGCAA TCGCACCATT 
AACAACAATG TGGAGCTGCT GCCTGCAACG AGCAGCACAC ATCAGACTTT GACCAGCGGC 
ACCACCATTG ACAAGGAGCT GCAGGACATC GCTCTGGGCG ATGGCATCCT GCCTGCCCCA 
GGCACCAAGG TGACCACCAC GGTGAGGACC TACACCTATG AGATCCCGGC CACTGGTCCC 
GGCAGCAACA CCAGCACCAT TAACCGCACC CACACGCTGA ACAACTCGAA TACGCTGAGC 
AGCACCTACA AGCACCACGA AAGCCACAAC TCCAGTCAGA ACTTCTCGCA GCTGTCGCCC 
GTTCCGCACC CACAGCCTAG CCATGTGCCA CAGACGGTGG TTTACAACAC TGAGAGCTAC 
TCCACGTTGA ACCGGAACAG CGATCGTCCG GTGGTTAGCA ACCAGTCGTA CGAGATCAGA 
GAGCACAAGG AGACCACCAC TCGCGGCGTG AGCCCACAGC CCCGTCAACT ACCTTTGGGA 
TCGGGTCCGG CGGGAACTCC ACCGGGCAAT CGTACGGTCA TCTACAACAT CCACAAGACG 
GATCACGTCA ACACAGTCAA CGAGCTGCCA CCACAGCAGC GCTATCCGCC GCGCAGTCCG 
GCTCACAGTC CCAACTACGG AGGACCACAC AGTCCACCAC TACAGCCGGG AGTGAATCGC 
TACTACTACA AGGAGACGGA GACCTCGAAC ACGGTCAATA GCATCCACGG ACCGCCGAAC 
GGTGGACCTT ACGAGCCGGG TCTACCTCAG TCCGCTCCGC TGAAACAGCT GCCGCCCAAC 
AACGCGTATC CACAGCAGCC ACCAAATGGA AATGGTTATC CACCGAATTC GACCACTTAC 
AGCTACAAAT ACTCCTCGAC CACCAACACA AACAACCGAC GTGGCCCGGA CTACGGCTCG 
CCATCTCCAT TCCCCGTGGA TGGCGTGGAG TATCCAGTGA ATAGCAATCC TCCCCAGCGA 
GTGGACGATC TGATGCAGTC ATTCGGAACT ACCACCGACC ATGTGGATCG CGTGGACATT 
GAGACTCCAG TGCGCAAGCG TGAAATCGAG ACGGCGGTTG CCTCGCCCAA CCAACAGCAT 
GTGCCATCGG TGAACAAGGC GGGAAAAGAG GTGTACTATC CACCCGGACA CGACACCATC 
GTCATGAAGC GCGAGGAGAT GCATGCTGGA TCTGCCTCAG GCGGTCGCTG GGCCAAGGGA 
TCCGGAATGT ACGAGTACGA GTCCGGCTCG AAGTCAAAGA CGAAAACCAA GTCCGGTGGC 
GCCGTTGTGC CCGTGTGCCT GCCCCTCTGC TGTGCCATGC CCTGCTCCAT TATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG8547 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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