Ack-like cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ack-like
Entrez Gene ID	36442
Full Name	Activated Cdc42 kinase-like
Synonyms	CG33007,CG3969,CG43741,DPR2,DRODPR2,DmHD-11,Dmel\CG43741,Dmel_CG33007,Dmel_CG3969,HD-11,PR2,PR2/ACK,Rp2,dACK
General protein information	Preferred Names Activated Cdc42 kinase-like Names CG43741 gene product from transcript CG43741-RA Ack-like-PA Ack-like-PC Ack-like-PD Ack-like-PE CG43741-PA CG43741-PC CG43741-PD CG43741-PE Fak-like tyrosine kinase activated Cdc42 kinase-like tyrosine kinase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 49F3-49F3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274006.1	NP_001260935.1	activated Cdc42 kinase-like, isoform E
NM_057738.4	NP_477086.2	activated Cdc42 kinase-like, isoform A
NM_001274005.1	NP_001260934.1	activated Cdc42 kinase-like, isoform C
NM_057739.4	NP_477087.3	activated Cdc42 kinase-like, isoform D

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007715	XP_308161.4
Drosophila melanogaster (fruit fly)	Ack-like	NP_477087.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ack-like gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ack-like cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09840	NM_001274006.1 Latest version!	Drosophila melanogaster activated Cdc42 kinase-like, transcript variant E (Ack-like), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa09841	NM_001274005.1 Latest version!	Drosophila melanogaster activated Cdc42 kinase-like, transcript variant C (Ack-like), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa12962	NM_057738.4 Latest version!	Drosophila melanogaster activated Cdc42 kinase-like, transcript variant A (Ack-like), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa12963	NM_057739.4 Latest version!	Drosophila melanogaster activated Cdc42 kinase-like, transcript variant D (Ack-like), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ack-like ( NM_001274006.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260935.1 Drosophila melanogaster activated Cdc42 kinase-like, transcript variant E (Ack-like), mRNA.

Price & Availability↑

CloneID	OFa09840
Clone ID Related Accession (Same CDS sequence)	NM_001274006.1
Accession Version	NM_001274006.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	activated Cdc42 kinase-like, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTATC CACAAATTGA TTTATACGAA TTCCTCACTG AGTCCGAACT GCAGCAGTAC 
TACAATGCCG TCAAAAACGA ATTGAAAATA ACGAACGCGG CCCAATTTAA GTATGCAGCG 
GACGAGGATT TGCGTTTCAT CGGACTCTCG CGCCCGGAAA TCCGGAGGCT GCGTAAGTTC 
TACGAGAAGC ATTTTCCCCA CAGCTACCTC AGCAAGATCA AGCGTCTGCT GCAGGCGCCT 
GGCACTATGG TTAAGCGGGA AGAGGCGCCC GGCGGCGGAA GTCAGGTGGC GCTGGATGGC 
AGTAGTGCCT CTGCATGCTC CTCTCTGGCC GCCAAGAACG GAGCCAGTTC TCCGAGCAAG 
GTGCCCAACA ACAAGCACAT TATACCGGCG GACTCCATTA GTGTAAACAA ACAGTTGGGA 
ACCGGCGAAT TTGGGATCGT CCAACAGGGA GTCTGGTCCA ATGGCAACGA ACGGATCCAA 
GTGGCTATTA AGTGCCTGTG CCGAGAGCGC ATGCAGTCAA ATCCCATGGA GTTCCTTAAG 
GAAGCGGCCA TCATGCATTC CATCGAGCAC GAGAATATCG TGCGCCTATA TGGCGTTGTC 
CTGGCCACTG ACTCGCTCAT GCTGGTCACT GAGCTCGCCC ATCTGAGATC ACTGTTGGAA 
TGTCTCAAGG ATTCGGGACT GCGAGTCAGT TTCCTCACCA TACCCACGCT CTGTGAGTTC 
GCCCTACAGA TCTGCAATGG AATGCGCTAT TTGGAACAGA AGCGCCTCAT CCACAGAGAC 
CTTGCGGCGC GAAATATTTT AGTGTTCAGC AAGGACAAGG TCAAGATCTC CGACTTTGGC 
CTGTCGCGGG CACTGGGCGT GGGCAAGGAC TACTACAAGA CCAATTTCAA CGTGAATTTA 
AAGCTGCCGA TCGCATGGTG TGCGCCCGAG TGTATCAATT ATCTGCGCTT TACCAACGCC 
TCAGATGTGT GGGCCTTTGG AGTTTGCCTG TGGGAAATGT TCTCCTACGG CTTTCAGCCC 
TGGGCGGCAC TTACCGGCTT GCAGATACTC GAGGCCATTG ATGCACCCAA CTACCAGCGG 
CTTGAACAGC CCGACTGCTG TCCCAGCGAG TATTATACAT TAATGATGAA GTGCTGGCAG 
GATGACGCTG CCAAGCGACC GCGCTTCGGT GAAATCTATG ACCAACTGCC CGATATGAAG 
CCAGAGCAGC TTAAAGCTGT AGTGAATTGC ACAGAACCCA AAAAGGACCA TCTACTTTAT 
CGCCAGGGCG ATATTATTAG CGTTCTAGAC CGCAATACGG GCACACCCTT CTGGAAAGGA 
GTGCTTAGCA CTGGCAAAAC AGGCTACTTC AACCCCTCCA ACACTGTGGC ATTCCTCGAA 
GGTCTGCCCA GCTCCACTCG TGATTCATTC AGCCGGGTGA GTGACCACAG AATCAGCAAA 
CGCAAGCTGC GCACTGAGAT GATATCGAAG CCACAGAACG ACTTCAAGCA CACCGGCCAC 
GTAGGTATTG ACGGAGCAAC ATTTGGAGAC ATCGCATTCC TGGGAAGTTC GCAGAATTAC 
AATCACGTAC CCAAACAGAT AGTGACGCCC TATAAACCCT CGGAGGACAT TGAACAAACA 
CCTCTCCTAC TGCCGCCCAC GCCCACTAGT CCTGACTCTC TTCAGACTGC CAGCGGGTAT 
TTCCCAGAGG GTGCCAACAG CGGAGGCGCC ATGGGCACCT CCATGAACCC CACCTTCATT 
CCATCCGCTG AACACACGCC CAAGCTGATA GCTACCAATG GCCAGAGTTC ATTCGACTTT 
GCCAGCGGGT CTACAAATCC GTTTTTTCCT AACAGAGGAG ACGATGAACT GGAGTTTGGC 
TTGCACAACT ATGGTGCGGA TGGGAAAAGT GTCCATTCGG AGACGGGCTG GCGCCCCACC 
TCTCGAAGTA TTGTAGATGA TCCTCATGAA TACCACGAGA TATCAGATGA TGAAATAGCG 
GCTGACAAGC TGGACTTTGG GCCCTCGCTG CTGGACGAGA TTAACTCGAT GTTTGGATCG 
ATAAGCGCTG CTACAGGCAG TCATCCCAAG TCACCCGGAT TTGATCATGT GAACAATAAA 
AATGAGATTA CGGAAATGTC CGCCAAATTG GGTCAAAAAA GTGGTGACAC CAATGGTAAT 
AAACATGGCC ATGGACTGCT GCCCACGCTC TCGAAAAAGA AATCGTCGGG AACTGTGAAA 
CCTATTTCTG TGAAGGATGA AAAGATTCTC AACCATGCCA TCGAGATAGC CAACGAAATT 
AGTGCTAGGT CGATGATTGA TCTGGTCTCT GATCAGACAC CTGTCATCCA CAGTCCTAAG 
CGCAAATTTA GTTTTAGGTT TCCGCACTTG AGTAATAATG GAAGCGGCGA CAAGTCTGGC 
GGATTAGGAA CCAGCGGCTC TGCCCACACG CCCACCCATG GCAATGCTTC GCCTTTTCCC 
AAGAAAAAGA ATTTCACCGA GGAACTGCAG AGCATTCCGG ATATACAGCG TTTCCGCTCG 
CTGAGCGAAA TCAAAAACAG CACCGATCTA CGTGTACCCA TCTTTGACAA GGAGAGCTCC 
GATTTTCTAT TTCAAAAGTC GCGCGAGATC CTTAGCAGGC CATTGAATCT GGAACGTGAG 
TGCGGGGATG AACAGCCGCC GAAGAGTATT GACGACAATA TCATGGACAT TGAAAACACA 
CTGAAAGCGC TCAACTTGGA CTTTATGCAC ACCTACACAG AGTTAGATAA AAGCGACTCC 
AACGACACGA TCCTCAACGA CCAGCTGCCG CACATGGCCA GTCTGGACGA CGGGATCAGC 
AGTGCTACCG GAAGCCTAAA GTCAGCGGTG TACAGCTACA GCAACGGTGT GCAAACCATG 
TTCGATTTTA ATGCGGCAAC CAGTCACCTG GACAAGCACC TCCAGTACAT GCATAACCAG 
GCCCAGCTAA GCGCCGCTGC CCCCGTCGAT CCCAAGCCTA TGGATGACAA ACGGTCGAGC 
ACTCCAGACA CGGGATTCGC ATCGCGCGAC ACTAACATCT CGTTGTCTCG TCGTAGCAGT 
CAAAAGTCCA GCTATAGTCC TCAGGAAAGT TTTCATTTTC CTTTGAAGGG CGAGCCCCTG 
TTTAGCGCAC CACCAGTGGT TATGTCAGGA GGATATGCCA GCCTCAAGGA TGAGCTCGGT 
TACGAGAGAT TCGGAAACGG AGCCACCAAG CAGATGCTGG CCAGCGCCTC GCCCATTAAG 
TCCGGAGTGG GCTTGAATGC TAGCAGTGGC TCTGTTTATA TGGGATCCAA GGGCTACCGC 
CAGCGTTCAA CATCATTCAA TGAGAGCTTC CATCCCATTT CACCGGTTCT CTCTGAACCG 
CCCCAGCGGG AGAGGGGCGT GATCCAGCGA CAAGTGAGGT TGGAGCAACA GCAGCCTTTG 
CCACGACGCT CCGCATCGTA TAAGCCCCGT TCGATCAGAG CTCGCACTCT AAGGCGATTA 
AGTTACAATC CAATGACCCT GGACTCGAGT TCATCCAGCG GCGAGGAGCC TGTCAAGCCA 
AGCCTGGCCC GTTCCGAGTG CGACATCCGT GCCAGAGTGG CGGCCAGCTC GAATTCGTCG 
TTGGGCAGAC GGCGCCGGGC GGGAATGAAT CGTCAGGTGC AGTCCGCATG CCACGACGAA 
CGTGAGGTTA TCATATCTCC TCGACAAATG TACGGATCCA ATTCGAGCAT CAAATCGGCG 
CCGCACTATA ATATTGGAGG GCAAAGACGC AGCTTTCACA GAGGAGGACC TGGTGTTATG 
GGTGGCGGCT ATGAGCAGTT CCAATACGAC GAGCGCATTT ACGATTTTGG AGGTCGTGGT 
CCGGGCAATA ACTACAACAT GGCCCAAGCG AGTTATTTGA GCTCTAGTCA AAAGCCCAGT 
TACCTTTTAA ATGGATCCGA ATTGCCCGGA TCCGGGTCGG GTTCGTCTTC AGCTGCTTCG 
TCGGCAGTGC AGGCCACCTA CAGGCCAGGA GCAGGAACCT CTGTACAGCG ACCAATGAGC 
GGTGGTGCTG CTCTGCACGA GTTCGACATT AGTCGTCTGA CGGGAAAGAG TCCCACTTCC 
ACCAACTTCG TGGGAAGTTC GCCCGAAGAA CTGAAACAGC GCCAGCTTTC TGTGGGCTCC 
CTTCAGACGC CAAATAACAA GGTAGCGAAG CCCCAGCGAC CCACGGAATT CCACTGGCCG 
GAGAAGATAC ATGCATCGGC GGTAAAGCAG CACGAGATGC ACTGGCGACA GGTGCACCAG 
CAGCAGTCGC TGCCCACAGC ATCGATTATC ACAGCAGCGG TTGGTGGAGC AGTTTCAGCC 
CGAAGATCTA GTGATAGCTC TTCCTCGTCG AGCACCGAGA GCGGGGATGA GTTCCAGTTT 
CGGCGCGAGT TTGTGGCGCC GCGAATTCCG CCCAGTCCGG CTCCATAACT AAGGAAAATG 
CTGAAATACG TTCTCAATTT ACTGTTTGGC AATGTGATGT CCGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260935.1
CDS	56..4543
Translation	MEYPQIDLYEFLTESELQQYYNAVKNELKITNAAQFKYAADEDLRFIGLSRPEIRRLRKFYEKHFPHSYLSKIKRLLQAPGTMVKREEAPGGGSQVALDGSSASACSSLAAKNGASSPSKVPNNKHIIPADSISVNKQLGTGEFGIVQQGVWSNGNERIQVAIKCLCRERMQSNPMEFLKEAAIMHSIEHENIVRLYGVVLATDSLMLVTELAHLRSLLECLKDSGLRVSFLTIPTLCEFALQICNGMRYLEQKRLIHRDLAARNILVFSKDKVKISDFGLSRALGVGKDYYKTNFNVNLKLPIAWCAPECINYLRFTNASDVWAFGVCLWEMFSYGFQPWAALTGLQILEAIDAPNYQRLEQPDCCPSEYYTLMMKCWQDDAAKRPRFGEIYDQLPDMKPEQLKAVVNCTEPKKDHLLYRQGDIISVLDRNTGTPFWKGVLSTGKTGYFNPSNTVAFLEGLPSSTRDSFSRVSDHRISKRKLRTEMISKPQNDFKHTGHVGIDGATFGDIAFLGSSQNYNHVPKQIVTPYKPSEDIEQTPLLLPPTPTSPDSLQTASGYFPEGANSGGAMGTSMNPTFIPSAEHTPKLIATNGQSSFDFASGSTNPFFPNRGDDELEFGLHNYGADGKSVHSETGWRPTSRSIVDDPHEYHEISDDEIAADKLDFGPSLLDEINSMFGSISAATGSHPKSPGFDHVNNKNEITEMSAKLGQKSGDTNGNKHGHGLLPTLSKKKSSGTVKPISVKDEKILNHAIEIANEISARSMIDLVSDQTPVIHSPKRKFSFRFPHLSNNGSGDKSGGLGTSGSAHTPTHGNASPFPKKKNFTEELQSIPDIQRFRSLSEIKNSTDLRVPIFDKESSDFLFQKSREILSRPLNLERECGDEQPPKSIDDNIMDIENTLKALNLDFMHTYTELDKSDSNDTILNDQLPHMASLDDGISSATGSLKSAVYSYSNGVQTMFDFNAATSHLDKHLQYMHNQAQLSAAAPVDPKPMDDKRSSTPDTGFASRDTNISLSRRSSQKSSYSPQESFHFPLKGEPLFSAPPVVMSGGYASLKDELGYERFGNGATKQMLASASPIKSGVGLNASSGSVYMGSKGYRQRSTSFNESFHPISPVLSEPPQRERGVIQRQVRLEQQQPLPRRSASYKPRSIRARTLRRLSYNPMTLDSSSSSGEEPVKPSLARSECDIRARVAASSNSSLGRRRRAGMNRQVQSACHDEREVIISPRQMYGSNSSIKSAPHYNIGGQRRSFHRGGPGVMGGGYEQFQYDERIYDFGGRGPGNNYNMAQASYLSSSQKPSYLLNGSELPGSGSGSSSAASSAVQATYRPGAGTSVQRPMSGGAALHEFDISRLTGKSPTSTNFVGSSPEELKQRQLSVGSLQTPNNKVAKPQRPTEFHWPEKIHASAVKQHEMHWRQVHQQQSLPTASIITAAVGGAVSARRSSDSSSSSSTESGDEFQFRREFVAPRIPPSPAP*LRKMLKYVLNLLFGNVMSD

Target ORF information:

RefSeq Version	NM_001274006.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster activated Cdc42 kinase-like, transcript variant E (Ack-like), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274006.1

ORF Insert Sequence:

ATGGAGTATC CACAAATTGA TTTATACGAA TTCCTCACTG AGTCCGAACT GCAGCAGTAC 
TACAATGCCG TCAAAAACGA ATTGAAAATA ACGAACGCGG CCCAATTTAA GTATGCAGCG 
GACGAGGATT TGCGTTTCAT CGGACTCTCG CGCCCGGAAA TCCGGAGGCT GCGTAAGTTC 
TACGAGAAGC ATTTTCCCCA CAGCTACCTC AGCAAGATCA AGCGTCTGCT GCAGGCGCCT 
GGCACTATGG TTAAGCGGGA AGAGGCGCCC GGCGGCGGAA GTCAGGTGGC GCTGGATGGC 
AGTAGTGCCT CTGCATGCTC CTCTCTGGCC GCCAAGAACG GAGCCAGTTC TCCGAGCAAG 
GTGCCCAACA ACAAGCACAT TATACCGGCG GACTCCATTA GTGTAAACAA ACAGTTGGGA 
ACCGGCGAAT TTGGGATCGT CCAACAGGGA GTCTGGTCCA ATGGCAACGA ACGGATCCAA 
GTGGCTATTA AGTGCCTGTG CCGAGAGCGC ATGCAGTCAA ATCCCATGGA GTTCCTTAAG 
GAAGCGGCCA TCATGCATTC CATCGAGCAC GAGAATATCG TGCGCCTATA TGGCGTTGTC 
CTGGCCACTG ACTCGCTCAT GCTGGTCACT GAGCTCGCCC ATCTGAGATC ACTGTTGGAA 
TGTCTCAAGG ATTCGGGACT GCGAGTCAGT TTCCTCACCA TACCCACGCT CTGTGAGTTC 
GCCCTACAGA TCTGCAATGG AATGCGCTAT TTGGAACAGA AGCGCCTCAT CCACAGAGAC 
CTTGCGGCGC GAAATATTTT AGTGTTCAGC AAGGACAAGG TCAAGATCTC CGACTTTGGC 
CTGTCGCGGG CACTGGGCGT GGGCAAGGAC TACTACAAGA CCAATTTCAA CGTGAATTTA 
AAGCTGCCGA TCGCATGGTG TGCGCCCGAG TGTATCAATT ATCTGCGCTT TACCAACGCC 
TCAGATGTGT GGGCCTTTGG AGTTTGCCTG TGGGAAATGT TCTCCTACGG CTTTCAGCCC 
TGGGCGGCAC TTACCGGCTT GCAGATACTC GAGGCCATTG ATGCACCCAA CTACCAGCGG 
CTTGAACAGC CCGACTGCTG TCCCAGCGAG TATTATACAT TAATGATGAA GTGCTGGCAG 
GATGACGCTG CCAAGCGACC GCGCTTCGGT GAAATCTATG ACCAACTGCC CGATATGAAG 
CCAGAGCAGC TTAAAGCTGT AGTGAATTGC ACAGAACCCA AAAAGGACCA TCTACTTTAT 
CGCCAGGGCG ATATTATTAG CGTTCTAGAC CGCAATACGG GCACACCCTT CTGGAAAGGA 
GTGCTTAGCA CTGGCAAAAC AGGCTACTTC AACCCCTCCA ACACTGTGGC ATTCCTCGAA 
GGTCTGCCCA GCTCCACTCG TGATTCATTC AGCCGGGTGA GTGACCACAG AATCAGCAAA 
CGCAAGCTGC GCACTGAGAT GATATCGAAG CCACAGAACG ACTTCAAGCA CACCGGCCAC 
GTAGGTATTG ACGGAGCAAC ATTTGGAGAC ATCGCATTCC TGGGAAGTTC GCAGAATTAC 
AATCACGTAC CCAAACAGAT AGTGACGCCC TATAAACCCT CGGAGGACAT TGAACAAACA 
CCTCTCCTAC TGCCGCCCAC GCCCACTAGT CCTGACTCTC TTCAGACTGC CAGCGGGTAT 
TTCCCAGAGG GTGCCAACAG CGGAGGCGCC ATGGGCACCT CCATGAACCC CACCTTCATT 
CCATCCGCTG AACACACGCC CAAGCTGATA GCTACCAATG GCCAGAGTTC ATTCGACTTT 
GCCAGCGGGT CTACAAATCC GTTTTTTCCT AACAGAGGAG ACGATGAACT GGAGTTTGGC 
TTGCACAACT ATGGTGCGGA TGGGAAAAGT GTCCATTCGG AGACGGGCTG GCGCCCCACC 
TCTCGAAGTA TTGTAGATGA TCCTCATGAA TACCACGAGA TATCAGATGA TGAAATAGCG 
GCTGACAAGC TGGACTTTGG GCCCTCGCTG CTGGACGAGA TTAACTCGAT GTTTGGATCG 
ATAAGCGCTG CTACAGGCAG TCATCCCAAG TCACCCGGAT TTGATCATGT GAACAATAAA 
AATGAGATTA CGGAAATGTC CGCCAAATTG GGTCAAAAAA GTGGTGACAC CAATGGTAAT 
AAACATGGCC ATGGACTGCT GCCCACGCTC TCGAAAAAGA AATCGTCGGG AACTGTGAAA 
CCTATTTCTG TGAAGGATGA AAAGATTCTC AACCATGCCA TCGAGATAGC CAACGAAATT 
AGTGCTAGGT CGATGATTGA TCTGGTCTCT GATCAGACAC CTGTCATCCA CAGTCCTAAG 
CGCAAATTTA GTTTTAGGTT TCCGCACTTG AGTAATAATG GAAGCGGCGA CAAGTCTGGC 
GGATTAGGAA CCAGCGGCTC TGCCCACACG CCCACCCATG GCAATGCTTC GCCTTTTCCC 
AAGAAAAAGA ATTTCACCGA GGAACTGCAG AGCATTCCGG ATATACAGCG TTTCCGCTCG 
CTGAGCGAAA TCAAAAACAG CACCGATCTA CGTGTACCCA TCTTTGACAA GGAGAGCTCC 
GATTTTCTAT TTCAAAAGTC GCGCGAGATC CTTAGCAGGC CATTGAATCT GGAACGTGAG 
TGCGGGGATG AACAGCCGCC GAAGAGTATT GACGACAATA TCATGGACAT TGAAAACACA 
CTGAAAGCGC TCAACTTGGA CTTTATGCAC ACCTACACAG AGTTAGATAA AAGCGACTCC 
AACGACACGA TCCTCAACGA CCAGCTGCCG CACATGGCCA GTCTGGACGA CGGGATCAGC 
AGTGCTACCG GAAGCCTAAA GTCAGCGGTG TACAGCTACA GCAACGGTGT GCAAACCATG 
TTCGATTTTA ATGCGGCAAC CAGTCACCTG GACAAGCACC TCCAGTACAT GCATAACCAG 
GCCCAGCTAA GCGCCGCTGC CCCCGTCGAT CCCAAGCCTA TGGATGACAA ACGGTCGAGC 
ACTCCAGACA CGGGATTCGC ATCGCGCGAC ACTAACATCT CGTTGTCTCG TCGTAGCAGT 
CAAAAGTCCA GCTATAGTCC TCAGGAAAGT TTTCATTTTC CTTTGAAGGG CGAGCCCCTG 
TTTAGCGCAC CACCAGTGGT TATGTCAGGA GGATATGCCA GCCTCAAGGA TGAGCTCGGT 
TACGAGAGAT TCGGAAACGG AGCCACCAAG CAGATGCTGG CCAGCGCCTC GCCCATTAAG 
TCCGGAGTGG GCTTGAATGC TAGCAGTGGC TCTGTTTATA TGGGATCCAA GGGCTACCGC 
CAGCGTTCAA CATCATTCAA TGAGAGCTTC CATCCCATTT CACCGGTTCT CTCTGAACCG 
CCCCAGCGGG AGAGGGGCGT GATCCAGCGA CAAGTGAGGT TGGAGCAACA GCAGCCTTTG 
CCACGACGCT CCGCATCGTA TAAGCCCCGT TCGATCAGAG CTCGCACTCT AAGGCGATTA 
AGTTACAATC CAATGACCCT GGACTCGAGT TCATCCAGCG GCGAGGAGCC TGTCAAGCCA 
AGCCTGGCCC GTTCCGAGTG CGACATCCGT GCCAGAGTGG CGGCCAGCTC GAATTCGTCG 
TTGGGCAGAC GGCGCCGGGC GGGAATGAAT CGTCAGGTGC AGTCCGCATG CCACGACGAA 
CGTGAGGTTA TCATATCTCC TCGACAAATG TACGGATCCA ATTCGAGCAT CAAATCGGCG 
CCGCACTATA ATATTGGAGG GCAAAGACGC AGCTTTCACA GAGGAGGACC TGGTGTTATG 
GGTGGCGGCT ATGAGCAGTT CCAATACGAC GAGCGCATTT ACGATTTTGG AGGTCGTGGT 
CCGGGCAATA ACTACAACAT GGCCCAAGCG AGTTATTTGA GCTCTAGTCA AAAGCCCAGT 
TACCTTTTAA ATGGATCCGA ATTGCCCGGA TCCGGGTCGG GTTCGTCTTC AGCTGCTTCG 
TCGGCAGTGC AGGCCACCTA CAGGCCAGGA GCAGGAACCT CTGTACAGCG ACCAATGAGC 
GGTGGTGCTG CTCTGCACGA GTTCGACATT AGTCGTCTGA CGGGAAAGAG TCCCACTTCC 
ACCAACTTCG TGGGAAGTTC GCCCGAAGAA CTGAAACAGC GCCAGCTTTC TGTGGGCTCC 
CTTCAGACGC CAAATAACAA GGTAGCGAAG CCCCAGCGAC CCACGGAATT CCACTGGCCG 
GAGAAGATAC ATGCATCGGC GGTAAAGCAG CACGAGATGC ACTGGCGACA GGTGCACCAG 
CAGCAGTCGC TGCCCACAGC ATCGATTATC ACAGCAGCGG TTGGTGGAGC AGTTTCAGCC 
CGAAGATCTA GTGATAGCTC TTCCTCGTCG AGCACCGAGA GCGGGGATGA GTTCCAGTTT 
CGGCGCGAGT TTGTGGCGCC GCGAATTCCG CCCAGTCCGG CTCCATAACT AAGGAAAATG 
CTGAAATACG TTCTCAATTT ACTGTTTGGC AATGTGATGT CCGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ack-like ( NM_001274005.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260934.1 Drosophila melanogaster activated Cdc42 kinase-like, transcript variant C (Ack-like), mRNA.

Price & Availability↑

CloneID	OFa09841
Clone ID Related Accession (Same CDS sequence)	NM_001274005.1
Accession Version	NM_001274005.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	activated Cdc42 kinase-like, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTATC CACAAATTGA TTTATACGAA TTCCTCACTG AGTCCGAACT GCAGCAGTAC 
TACAATGCCG TCAAAAACGA ATTGAAAATA ACGAACGCGG CCCAATTTAA GTATGCAGCG 
GACGAGGATT TGCGTTTCAT CGGACTCTCG CGCCCGGAAA TCCGGAGGCT GCGTAAGTTC 
TACGAGAAGC ATTTTCCCCA CAGCTACCTC AGCAAGATCA AGCGTCTGCT GCAGGCGCCT 
GGCACTATGG TTAAGCGGGA AGAGGCGCCC GGCGGCGGAA GTCAGGTGGC GCTGGATGGC 
AGTAGTGCCT CTGCATGCTC CTCTCTGGCC GCCAAGAACG GAGCCAGTTC TCCGAGCAAG 
GTGCCCAACA ACAAGCACAT TATACCGGCG GACTCCATTA GTGTAAACAA ACAGTTGGGA 
ACCGGCGAAT TTGGGATCGT CCAACAGGGA GTCTGGTCCA ATGGCAACGA ACGGATCCAA 
GTGGCTATTA AGTGCCTGTG CCGAGAGCGC ATGCAGTCAA ATCCCATGGA GTTCCTTAAG 
GAAGCGGCCA TCATGCATTC CATCGAGCAC GAGAATATCG TGCGCCTATA TGGCGTTGTC 
CTGGCCACTG ACTCGCTCAT GCTGGTCACT GAGCTCGCCC ATCTGAGATC ACTGTTGGAA 
TGTCTCAAGG ATTCGGGACT GCGAGTCAGT TTCCTCACCA TACCCACGCT CTGTGAGTTC 
GCCCTACAGA TCTGCAATGG AATGCGCTAT TTGGAACAGA AGCGCCTCAT CCACAGAGAC 
CTTGCGGCGC GAAATATTTT AGTGTTCAGC AAGGACAAGG TCAAGATCTC CGACTTTGGC 
CTGTCGCGGG CACTGGGCGT GGGCAAGGAC TACTACAAGA CCAATTTCAA CGTGAATTTA 
AAGCTGCCGA TCGCATGGTG TGCGCCCGAG TGTATCAATT ATCTGCGCTT TACCAACGCC 
TCAGATGTGT GGGCCTTTGG AGTTTGCCTG TGGGAAATGT TCTCCTACGG CTTTCAGCCC 
TGGGCGGCAC TTACCGGCTT GCAGATACTC GAGGCCATTG ATGCACCCAA CTACCAGCGG 
CTTGAACAGC CCGACTGCTG TCCCAGCGAG TATTATACAT TAATGATGAA GTGCTGGCAG 
GATGACGCTG CCAAGCGACC GCGCTTCGGT GAAATCTATG ACCAACTGCC CGATATGAAG 
CCAGAGCAGC TTAAAGCTGT AGTGAATTGC ACAGAACCCA AAAAGGACCA TCTACTTTAT 
CGCCAGGGCG ATATTATTAG CGTTCTAGAC CGCAATACGG GCACACCCTT CTGGAAAGGA 
GTGCTTAGCA CTGGCAAAAC AGGCTACTTC AACCCCTCCA ACACTGTGGC ATTCCTCGAA 
GGTCTGCCCA GCTCCACTCG TGATTCATTC AGCCGGGTGA GTGACCACAG AATCAGCAAA 
CGCAAGCTGC GCACTGAGAT GATATCGAAG CCACAGAACG ACTTCAAGCA CACCGGCCAC 
GTAGGTATTG ACGGAGCAAC ATTTGGAGAC ATCGCATTCC TGGGAAGTTC GCAGAATTAC 
AATCACGTAC CCAAACAGAT AGTGACGCCC TATAAACCCT CGGAGGACAT TGAACAAACA 
CCTCTCCTAC TGCCGCCCAC GCCCACTAGT CCTGACTCTC TTCAGACTGC CAGCGGGTAT 
TTCCCAGAGG GTGCCAACAG CGGAGGCGCC ATGGGCACCT CCATGAACCC CACCTTCATT 
CCATCCGCTG AACACACGCC CAAGCTGATA GCTACCAATG GCCAGAGTTC ATTCGACTTT 
GCCAGCGGGT CTACAAATCC GTTTTTTCCT AACAGAGGAG ACGATGAACT GGAGTTTGGC 
TTGCACAACT ATGGTGCGGA TGGGAAAAGT GTCCATTCGG AGACGGGCTG GCGCCCCACC 
TCTCGAAGTA TTGTAGATGA TCCTCATGAA TACCACGAGA TATCAGATGA TGAAATAGCG 
GCTGACAAGC TGGACTTTGG GCCCTCGCTG CTGGACGAGA TTAACTCGAT GTTTGGATCG 
ATAAGCGCTG CTACAGGCAG TCATCCCAAG TCACCCGGAT TTGATCATGT GAACAATAAA 
AATGAGATTA CGGAAATGTC CGCCAAATTG GGTCAAAAAA GTGGTGACAC CAATGGTAAT 
AAACATGGCC ATGGACTGCT GCCCACGCTC TCGAAAAAGA AATCGTCGGG AACTGTGAAA 
CCTATTTCTG TGAAGGATGA AAAGATTCTC AACCATGCCA TCGAGATAGC CAACGAAATT 
AGTGCTAGGT CGATGATTGA TCTGGTCTCT GATCAGACAC CTGTCATCCA CAGTCCTAAG 
CGCAAATTTA GTTTTAGGTT TCCGCACTTG AGTAATAATG GAAGCGGCGA CAAGTCTGGC 
GGATTAGGAA CCAGCGGCTC TGCCCACACG CCCACCCATG GCAATGCTTC GCCTTTTCCC 
AAGAAAAAGA ATTTCACCGA GGAACTGCAG AGCATTCCGG ATATACAGCG TTTCCGCTCG 
CTGAGCGAAA TCAAAAACAG CACCGATCTA CGTGTACCCA TCTTTGACAA GGAGAGCTCC 
GATTTTCTAT TTCAAAAGTC GCGCGAGATC CTTAGCAGGC CATTGAATCT GGAACGTGAG 
TGCGGGGATG AACAGCCGCC GAAGAGTATT GACGACAATA TCATGGACAT TGAAAACACA 
CTGAAAGCGC TCAACTTGGA CTTTATGCAC ACCTACACAG AGTTAGATAA AAGCGACTCC 
AACGACACGA TCCTCAACGA CCAGCTGCCG CACATGGCCA GTCTGGACGA CGGGATCAGC 
AGTGCTACCG GAAGCCTAAA GTCAGCGGTG TACAGCTACA GCAACGGTGT GCAAACCATG 
TTCGATTTTA ATGCGGCAAC CAGTCACCTG GACAAGCACC TCCAGTACAT GCATAACCAG 
GCCCAGCTAA GCGCCGCTGC CCCCGTCGAT CCCAAGCCTA TGGATGACAA ACGGTCGAGC 
ACTCCAGACA CGGGATTCGC ATCGCGCGAC ACTAACATCT CGTTGTCTCG TCGTAGCAGT 
CAAAAGTCCA GCTATAGTCC TCAGGAAAGT TTTCATTTTC CTTTGAAGGG CGAGCCCCTG 
TTTAGCGCAC CACCAGTGGT TATGTCAGGA GGATATGCCA GCCTCAAGGA TGAGCTCGGT 
TACGAGAGAT TCGGAAACGG AGCCACCAAG CAGATGCTGG CCAGCGCCTC GCCCATTAAG 
TCCGGAGTGG GCTTGAATGC TAGCAGTGGC TCTGTTTATA TGGGATCCAA GGGCTACCGC 
CAGCGTTCAA CATCATTCAA TGAGAGCTTC CATCCCATTT CACCGGTTCT CTCTGAACCG 
CCCCAGCGGG AGAGGGGCGT GATCCAGCGA CAAGTGAGGT TGGAGCAACA GCAGCCTTTG 
CCACGACGCT CCGCATCGTA TAAGCCCCGT TCGATCAGAG CTCGCACTCT AAGGCGATTA 
AGTTACAATC CAATGACCCT GGACTCGAGT TCATCCAGCG GCGAGGAGCC TGTCAAGCCA 
AGCCTGGCCC GTTCCGAGTG CGACATCCGT GCCAGAGTGG CGGCCAGCTC GAATTCGTCG 
TTGGGCAGAC GGCGCCGGGC GGGAATGAAT CGTCAGGTGC AGTCCGCATG CCACGACGAA 
CGTGAGGTTA TCATATCTCC TCGACAAATG TACGGATCCA ATTCGAGCAT CAAATCGGCG 
CCGCACTATA ATATTGGAGG GCAAAGACGC AGCTTTCACA GAGGAGGACC TGGTGTTATG 
GGTGGCGGCT ATGAGCAGTT CCAATACGAC GAGCGCATTT ACGATTTTGG AGGTCGTGGT 
CCGGGCAATA ACTACAACAT GGCCCAAGCG AGTTATTTGA GCTCTAGTCA AAAGCCCAGT 
TACCTTTTAA ATGGATCCGA ATTGCCCGGA TCCGGGTCGG GTTCGTCTTC AGCTGCTTCG 
TCGGCAGTGC AGGCCACCTA CAGGCCAGGA GCAGGAACCT CTGTACAGCG ACCAATGAGC 
GGTGGTGCTG CTCTGCACGA GTTCGACATT AGTCGTCTGA CGGGAAAGAG TCCCACTTCC 
ACCAACTTCG TGGGAAGTTC GCCCGAAGAA CTGAAACAGC GCCAGCTTTC TGTGGGCTCC 
CTTCAGACGC CAAATAACAA GGTAGCGAAG CCCCAGCGAC CCACGGAATT CCACTGGCCG 
GAGAAGATAC ATGCATCGGC GGTAAAGCAG CACGAGATGC ACTGGCGACA GGTGCACCAG 
CAGCAGTCGC TGCCCACAGC ATCGATTATC ACAGCAGCGG TTGGTGGAGC AGTTTCAGCC 
CGAAGATCTA GTGATAGCTC TTCCTCGTCG AGCACCGAGA GCGGGGATGA GTTCCAGTTT 
CGGCGCGAGT TTGTGGCGCC GCGAATTCCG CCCAGTCCGG CTCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260934.1
CDS	56..4483
Translation	MEYPQIDLYEFLTESELQQYYNAVKNELKITNAAQFKYAADEDLRFIGLSRPEIRRLRKFYEKHFPHSYLSKIKRLLQAPGTMVKREEAPGGGSQVALDGSSASACSSLAAKNGASSPSKVPNNKHIIPADSISVNKQLGTGEFGIVQQGVWSNGNERIQVAIKCLCRERMQSNPMEFLKEAAIMHSIEHENIVRLYGVVLATDSLMLVTELAHLRSLLECLKDSGLRVSFLTIPTLCEFALQICNGMRYLEQKRLIHRDLAARNILVFSKDKVKISDFGLSRALGVGKDYYKTNFNVNLKLPIAWCAPECINYLRFTNASDVWAFGVCLWEMFSYGFQPWAALTGLQILEAIDAPNYQRLEQPDCCPSEYYTLMMKCWQDDAAKRPRFGEIYDQLPDMKPEQLKAVVNCTEPKKDHLLYRQGDIISVLDRNTGTPFWKGVLSTGKTGYFNPSNTVAFLEGLPSSTRDSFSRVSDHRISKRKLRTEMISKPQNDFKHTGHVGIDGATFGDIAFLGSSQNYNHVPKQIVTPYKPSEDIEQTPLLLPPTPTSPDSLQTASGYFPEGANSGGAMGTSMNPTFIPSAEHTPKLIATNGQSSFDFASGSTNPFFPNRGDDELEFGLHNYGADGKSVHSETGWRPTSRSIVDDPHEYHEISDDEIAADKLDFGPSLLDEINSMFGSISAATGSHPKSPGFDHVNNKNEITEMSAKLGQKSGDTNGNKHGHGLLPTLSKKKSSGTVKPISVKDEKILNHAIEIANEISARSMIDLVSDQTPVIHSPKRKFSFRFPHLSNNGSGDKSGGLGTSGSAHTPTHGNASPFPKKKNFTEELQSIPDIQRFRSLSEIKNSTDLRVPIFDKESSDFLFQKSREILSRPLNLERECGDEQPPKSIDDNIMDIENTLKALNLDFMHTYTELDKSDSNDTILNDQLPHMASLDDGISSATGSLKSAVYSYSNGVQTMFDFNAATSHLDKHLQYMHNQAQLSAAAPVDPKPMDDKRSSTPDTGFASRDTNISLSRRSSQKSSYSPQESFHFPLKGEPLFSAPPVVMSGGYASLKDELGYERFGNGATKQMLASASPIKSGVGLNASSGSVYMGSKGYRQRSTSFNESFHPISPVLSEPPQRERGVIQRQVRLEQQQPLPRRSASYKPRSIRARTLRRLSYNPMTLDSSSSSGEEPVKPSLARSECDIRARVAASSNSSLGRRRRAGMNRQVQSACHDEREVIISPRQMYGSNSSIKSAPHYNIGGQRRSFHRGGPGVMGGGYEQFQYDERIYDFGGRGPGNNYNMAQASYLSSSQKPSYLLNGSELPGSGSGSSSAASSAVQATYRPGAGTSVQRPMSGGAALHEFDISRLTGKSPTSTNFVGSSPEELKQRQLSVGSLQTPNNKVAKPQRPTEFHWPEKIHASAVKQHEMHWRQVHQQQSLPTASIITAAVGGAVSARRSSDSSSSSSTESGDEFQFRREFVAPRIPPSPAP

Target ORF information:

RefSeq Version	NM_001274005.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster activated Cdc42 kinase-like, transcript variant C (Ack-like), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274005.1

ORF Insert Sequence:

ATGGAGTATC CACAAATTGA TTTATACGAA TTCCTCACTG AGTCCGAACT GCAGCAGTAC 
TACAATGCCG TCAAAAACGA ATTGAAAATA ACGAACGCGG CCCAATTTAA GTATGCAGCG 
GACGAGGATT TGCGTTTCAT CGGACTCTCG CGCCCGGAAA TCCGGAGGCT GCGTAAGTTC 
TACGAGAAGC ATTTTCCCCA CAGCTACCTC AGCAAGATCA AGCGTCTGCT GCAGGCGCCT 
GGCACTATGG TTAAGCGGGA AGAGGCGCCC GGCGGCGGAA GTCAGGTGGC GCTGGATGGC 
AGTAGTGCCT CTGCATGCTC CTCTCTGGCC GCCAAGAACG GAGCCAGTTC TCCGAGCAAG 
GTGCCCAACA ACAAGCACAT TATACCGGCG GACTCCATTA GTGTAAACAA ACAGTTGGGA 
ACCGGCGAAT TTGGGATCGT CCAACAGGGA GTCTGGTCCA ATGGCAACGA ACGGATCCAA 
GTGGCTATTA AGTGCCTGTG CCGAGAGCGC ATGCAGTCAA ATCCCATGGA GTTCCTTAAG 
GAAGCGGCCA TCATGCATTC CATCGAGCAC GAGAATATCG TGCGCCTATA TGGCGTTGTC 
CTGGCCACTG ACTCGCTCAT GCTGGTCACT GAGCTCGCCC ATCTGAGATC ACTGTTGGAA 
TGTCTCAAGG ATTCGGGACT GCGAGTCAGT TTCCTCACCA TACCCACGCT CTGTGAGTTC 
GCCCTACAGA TCTGCAATGG AATGCGCTAT TTGGAACAGA AGCGCCTCAT CCACAGAGAC 
CTTGCGGCGC GAAATATTTT AGTGTTCAGC AAGGACAAGG TCAAGATCTC CGACTTTGGC 
CTGTCGCGGG CACTGGGCGT GGGCAAGGAC TACTACAAGA CCAATTTCAA CGTGAATTTA 
AAGCTGCCGA TCGCATGGTG TGCGCCCGAG TGTATCAATT ATCTGCGCTT TACCAACGCC 
TCAGATGTGT GGGCCTTTGG AGTTTGCCTG TGGGAAATGT TCTCCTACGG CTTTCAGCCC 
TGGGCGGCAC TTACCGGCTT GCAGATACTC GAGGCCATTG ATGCACCCAA CTACCAGCGG 
CTTGAACAGC CCGACTGCTG TCCCAGCGAG TATTATACAT TAATGATGAA GTGCTGGCAG 
GATGACGCTG CCAAGCGACC GCGCTTCGGT GAAATCTATG ACCAACTGCC CGATATGAAG 
CCAGAGCAGC TTAAAGCTGT AGTGAATTGC ACAGAACCCA AAAAGGACCA TCTACTTTAT 
CGCCAGGGCG ATATTATTAG CGTTCTAGAC CGCAATACGG GCACACCCTT CTGGAAAGGA 
GTGCTTAGCA CTGGCAAAAC AGGCTACTTC AACCCCTCCA ACACTGTGGC ATTCCTCGAA 
GGTCTGCCCA GCTCCACTCG TGATTCATTC AGCCGGGTGA GTGACCACAG AATCAGCAAA 
CGCAAGCTGC GCACTGAGAT GATATCGAAG CCACAGAACG ACTTCAAGCA CACCGGCCAC 
GTAGGTATTG ACGGAGCAAC ATTTGGAGAC ATCGCATTCC TGGGAAGTTC GCAGAATTAC 
AATCACGTAC CCAAACAGAT AGTGACGCCC TATAAACCCT CGGAGGACAT TGAACAAACA 
CCTCTCCTAC TGCCGCCCAC GCCCACTAGT CCTGACTCTC TTCAGACTGC CAGCGGGTAT 
TTCCCAGAGG GTGCCAACAG CGGAGGCGCC ATGGGCACCT CCATGAACCC CACCTTCATT 
CCATCCGCTG AACACACGCC CAAGCTGATA GCTACCAATG GCCAGAGTTC ATTCGACTTT 
GCCAGCGGGT CTACAAATCC GTTTTTTCCT AACAGAGGAG ACGATGAACT GGAGTTTGGC 
TTGCACAACT ATGGTGCGGA TGGGAAAAGT GTCCATTCGG AGACGGGCTG GCGCCCCACC 
TCTCGAAGTA TTGTAGATGA TCCTCATGAA TACCACGAGA TATCAGATGA TGAAATAGCG 
GCTGACAAGC TGGACTTTGG GCCCTCGCTG CTGGACGAGA TTAACTCGAT GTTTGGATCG 
ATAAGCGCTG CTACAGGCAG TCATCCCAAG TCACCCGGAT TTGATCATGT GAACAATAAA 
AATGAGATTA CGGAAATGTC CGCCAAATTG GGTCAAAAAA GTGGTGACAC CAATGGTAAT 
AAACATGGCC ATGGACTGCT GCCCACGCTC TCGAAAAAGA AATCGTCGGG AACTGTGAAA 
CCTATTTCTG TGAAGGATGA AAAGATTCTC AACCATGCCA TCGAGATAGC CAACGAAATT 
AGTGCTAGGT CGATGATTGA TCTGGTCTCT GATCAGACAC CTGTCATCCA CAGTCCTAAG 
CGCAAATTTA GTTTTAGGTT TCCGCACTTG AGTAATAATG GAAGCGGCGA CAAGTCTGGC 
GGATTAGGAA CCAGCGGCTC TGCCCACACG CCCACCCATG GCAATGCTTC GCCTTTTCCC 
AAGAAAAAGA ATTTCACCGA GGAACTGCAG AGCATTCCGG ATATACAGCG TTTCCGCTCG 
CTGAGCGAAA TCAAAAACAG CACCGATCTA CGTGTACCCA TCTTTGACAA GGAGAGCTCC 
GATTTTCTAT TTCAAAAGTC GCGCGAGATC CTTAGCAGGC CATTGAATCT GGAACGTGAG 
TGCGGGGATG AACAGCCGCC GAAGAGTATT GACGACAATA TCATGGACAT TGAAAACACA 
CTGAAAGCGC TCAACTTGGA CTTTATGCAC ACCTACACAG AGTTAGATAA AAGCGACTCC 
AACGACACGA TCCTCAACGA CCAGCTGCCG CACATGGCCA GTCTGGACGA CGGGATCAGC 
AGTGCTACCG GAAGCCTAAA GTCAGCGGTG TACAGCTACA GCAACGGTGT GCAAACCATG 
TTCGATTTTA ATGCGGCAAC CAGTCACCTG GACAAGCACC TCCAGTACAT GCATAACCAG 
GCCCAGCTAA GCGCCGCTGC CCCCGTCGAT CCCAAGCCTA TGGATGACAA ACGGTCGAGC 
ACTCCAGACA CGGGATTCGC ATCGCGCGAC ACTAACATCT CGTTGTCTCG TCGTAGCAGT 
CAAAAGTCCA GCTATAGTCC TCAGGAAAGT TTTCATTTTC CTTTGAAGGG CGAGCCCCTG 
TTTAGCGCAC CACCAGTGGT TATGTCAGGA GGATATGCCA GCCTCAAGGA TGAGCTCGGT 
TACGAGAGAT TCGGAAACGG AGCCACCAAG CAGATGCTGG CCAGCGCCTC GCCCATTAAG 
TCCGGAGTGG GCTTGAATGC TAGCAGTGGC TCTGTTTATA TGGGATCCAA GGGCTACCGC 
CAGCGTTCAA CATCATTCAA TGAGAGCTTC CATCCCATTT CACCGGTTCT CTCTGAACCG 
CCCCAGCGGG AGAGGGGCGT GATCCAGCGA CAAGTGAGGT TGGAGCAACA GCAGCCTTTG 
CCACGACGCT CCGCATCGTA TAAGCCCCGT TCGATCAGAG CTCGCACTCT AAGGCGATTA 
AGTTACAATC CAATGACCCT GGACTCGAGT TCATCCAGCG GCGAGGAGCC TGTCAAGCCA 
AGCCTGGCCC GTTCCGAGTG CGACATCCGT GCCAGAGTGG CGGCCAGCTC GAATTCGTCG 
TTGGGCAGAC GGCGCCGGGC GGGAATGAAT CGTCAGGTGC AGTCCGCATG CCACGACGAA 
CGTGAGGTTA TCATATCTCC TCGACAAATG TACGGATCCA ATTCGAGCAT CAAATCGGCG 
CCGCACTATA ATATTGGAGG GCAAAGACGC AGCTTTCACA GAGGAGGACC TGGTGTTATG 
GGTGGCGGCT ATGAGCAGTT CCAATACGAC GAGCGCATTT ACGATTTTGG AGGTCGTGGT 
CCGGGCAATA ACTACAACAT GGCCCAAGCG AGTTATTTGA GCTCTAGTCA AAAGCCCAGT 
TACCTTTTAA ATGGATCCGA ATTGCCCGGA TCCGGGTCGG GTTCGTCTTC AGCTGCTTCG 
TCGGCAGTGC AGGCCACCTA CAGGCCAGGA GCAGGAACCT CTGTACAGCG ACCAATGAGC 
GGTGGTGCTG CTCTGCACGA GTTCGACATT AGTCGTCTGA CGGGAAAGAG TCCCACTTCC 
ACCAACTTCG TGGGAAGTTC GCCCGAAGAA CTGAAACAGC GCCAGCTTTC TGTGGGCTCC 
CTTCAGACGC CAAATAACAA GGTAGCGAAG CCCCAGCGAC CCACGGAATT CCACTGGCCG 
GAGAAGATAC ATGCATCGGC GGTAAAGCAG CACGAGATGC ACTGGCGACA GGTGCACCAG 
CAGCAGTCGC TGCCCACAGC ATCGATTATC ACAGCAGCGG TTGGTGGAGC AGTTTCAGCC 
CGAAGATCTA GTGATAGCTC TTCCTCGTCG AGCACCGAGA GCGGGGATGA GTTCCAGTTT 
CGGCGCGAGT TTGTGGCGCC GCGAATTCCG CCCAGTCCGG CTCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ack-like ( NM_057738.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477086.2 Drosophila melanogaster activated Cdc42 kinase-like, transcript variant A (Ack-like), mRNA.

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CloneID	OFa12962
Clone ID Related Accession (Same CDS sequence)	NM_057738.4
Accession Version	NM_057738.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	activated Cdc42 kinase-like, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_057738.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTATC CACAAATTGA TTTATACGAA TTCCTCACTG AGTCCGAACT GCAGCAGTAC 
TACAATGCCG TCAAAAACGA ATTGAAAATA ACGAACGCGG CCCAATTTAA GTATGCAGCG 
GACGAGGATT TGCGTTTCAT CGGACTCTCG CGCCCGGAAA TCCGGAGGCT GCGTAAGTTC 
TACGAGAAGC ATTTTCCCCA CAGCTACCTC AGCAAGATCA AGCGTCTGCT GCAGGCGCCT 
GGCACTATGG TTAAGCGGGA AGAGGCGCCC GGCGGCGGAA GTCAGGTGGC GCTGGATGGC 
AGTAGTGCCT CTGCATGCTC CTCTCTGGCC GCCAAGAACG GAGCCAGTTC TCCGAGCAAG 
GTGCCCAACA ACAAGCACAT TATACCGGCG GACTCCATTA GTGTAAACAA ACAGTTGGGA 
ACCGGCGAAT TTGGGATCGT CCAACAGGGA GTCTGGTCCA ATGGCAACGA ACGGATCCAA 
GTGGCTATTA AGTGCCTGTG CCGAGAGCGC ATGCAGTCAA ATCCCATGGA GTTCCTTAAG 
GAAGCGGCCA TCATGCATTC CATCGAGCAC GAGAATATCG TGCGCCTATA TGGCGTTGTC 
CTGGCCACTG ACTCGCTCAT GCTGGTCACT GAGCTCGCCC ATCTGAGATC ACTGTTGGAA 
TGTCTCAAGG ATTCGGGACT GCGAGTCAGT TTCCTCACCA TACCCACGCT CTGTGAGTTC 
GCCCTACAGA TCTGCAATGG AATGCGCTAT TTGGAACAGA AGCGCCTCAT CCACAGAGAC 
CTTGCGGCGC GAAATATTTT AGTGTTCAGC AAGGACAAGG TCAAGATCTC CGACTTTGGC 
CTGTCGCGGG CACTGGGCGT GGGCAAGGAC TACTACAAGA CCAATTTCAA CGTGAATTTA 
AAGCTGCCGA TCGCATGGTG TGCGCCCGAG TGTATCAATT ATCTGCGCTT TACCAACGCC 
TCAGATGTGT GGGCCTTTGG AGTTTGCCTG TGGGAAATGT TCTCCTACGG CTTTCAGCCC 
TGGGCGGCAC TTACCGGCTT GCAGATACTC GAGGCCATTG ATGCACCCAA CTACCAGCGG 
CTTGAACAGC CCGACTGCTG TCCCAGCGAG TATTATACAT TAATGATGAA GTGCTGGCAG 
GATGACGCTG CCAAGCGACC GCGCTTCGGT GAAATCTATG ACCAACTGCC CGATATGAAG 
CCAGAGCAGC TTAAAGCTGT AGTGAATTGC ACAGAACCCA AAAAGGACCA TCTACTTTAT 
CGCCAGGGCG ATATTATTAG CGTTCTAGAC CGCAATACGG GCACACCCTT CTGGAAAGGA 
GTGCTTAGCA CTGGCAAAAC AGGCTACTTC AACCCCTCCA ACACTGTGGC ATTCCTCGAA 
GGTCTGCCCA GCTCCACTCG TGATTCATTC AGCCGGGTGA GTGACCACAG AATCAGCAAA 
CGCAAGCTGC GCACTGAGAT GATATCGAAG CCACAGAACG ACTTCAAGCA CACCGGCCAC 
GTAGGTATTG ACGGAGCAAC ATTTGGAGAC ATCGCATTCC TGGGAAGTTC GCAGAATTAC 
AATCACGTAC CCAAACAGAT AGTGACGCCC TATAAACCCT CGGAGGACAT TGAACAAACA 
CCTCTCCTAC TGCCGCCCAC GCCCACTAGT CCTGACTCTC TTCAGACTGC CAGCGGGTAT 
TTCCCAGAGG GTGCCAACAG CGGAGGCGCC ATGGGCACCT CCATGAACCC CACCTTCATT 
CCATCCGCTG AACACACGCC CAAGCTGATA GCTACCAATG GCCAGAGTTC ATTCGACTTT 
GCCAGCGGGT CTACAAATCC GTTTTTTCCT AACAGAGGAG ACGATGAACT GGAGTTTGGC 
TTGCACAACT ATGGTGCGGA TGGGAAAAGT GTCCATTCGG AGACGGGCTG GCGCCCCACC 
TCTCGAAGTA TTGTAGATGA TCCTCATGAA TACCACGAGA TATCAGATGA TGAAATAGCG 
GCTGACAAGC TGGACTTTGG GCCCTCGCTG CTGGACGAGA TTAACTCGAT GTTTGGATCG 
ATAAGCGCTG CTACAGGCAG TCATCCCAAG TCACCCGGAT TTGATCATGT GAACAATAAA 
AATGAGATTA CGGAAATGTC CGCCAAATTG GGTCAAAAAA GTGGTGACAC CAATGGTAAT 
AAACATGGCC ATGGACTGCT GCCCACGCTC TCGAAAAAGA AATCGTCGGG AACTGTGAAA 
CCTATTTCTG TGAAGGATGA AAAGATTCTC AACCATGCCA TCGAGATAGC CAACGAAATT 
AGTGCTAGGT CGATGATTGA TCTGGTCTCT GATCAGACAC CTGTCATCCA CAGTCCTAAG 
CGCAAATTTA GTTTTAGGTT TCCGCACTTG AGTAATAATG GAAGCGGCGA CAAGTCTGGC 
GGATTAGGAA CCAGCGGCTC TGCCCACACG CCCACCCATG GCAATGCTTC GCCTTTTCCC 
AAGAAAAAGA ATTTCACCGA GGAACTGCAG AGCATTCCGG ATATACAGTC GCTGATCGGC 
AAGGAGGGAC TGGAGGCCTA CAACAGCCTG ATCGAGCGCA AGGCCCTGCT GGACATTGGT 
CCCAGTCCGG CGGCCACCTT GCTCCGCCAC CTGGACACCG ACGAGTTCGA TCTTCAAAGC 
CTTCACCAGT CACAGCGCCC AATGACATTG CCCACTCGGG GTGCCACCCA GCGGGTGCGC 
AAGGCTGAAC TGGCCGCCGG CCTGAGTCGG CACAATGACG AGAACTCGAA TTCGCTGGAG 
GCTTGCGAGA GTCCCAGCTA CATGACCCAC GGCAGCTACA AGTTTCCCGA GGCACAGCCG 
ACAGAACAGC TTCCGGAGCC GGAGTCGCCG AATCCCATAC CCTTGCCGCC GCGCGAGGGC 
AAAAAGCAGG TGAAGACCAG CACCAAGCGG CATGTGCGCA AGTATCCGTT GATAATCCCG 
GCCAACGGTC TGCAACGCAC CCTCAGCAAG CTGGCGGACT TTGGTGACGA GGCTGCCAAA 
TCACCGGAGA TCTCCACATC TTCTCAGCCA CAGCCAGGAC GCGCCATCGA AGTAGTGGCC 
GCTGTCCGGC CGAGTGGCAT GCGACGTCCA TCGCGACCCT CGGAGCGGGA ATACGAGAAC 
ATGCCGACAG TGGGTAAAGA ATCCGCACAC ACCTACCAGA ACTTGGATAA GCTGACACCT 
GCGGATGCTG CCGGATTAAC GGATACAGCT TCGTTGCAGT TCGAGTCCAT CATGGAAGCT 
GACACCAGTA AGGAGGGCAT TCTGCAGTCG CCGGATGTAA CCGACGGATT CTACAACTTT 
TCCATTCAGA AGGAGCACTA CAACAAGGGC AAAGACGCCG AGTTTGAGGC CACTCAGATA 
TCCGGCCTGT ATGTGAACGA TGACGAACTA CGTAACCTGG ACATCGAGAG CAGTCGGCGC 
ACAGCCACTC CATGCAGTAG TTGCAGCGCT TTGGAATCGG AGCACAGCCA ACCAGACGCG 
TTGCCGTCAA CAGAGTCTGT GTCGGAAGTG AGTCGCTTCT CAAGTGTGGA CAATGAGCTG 
GCTGGAAATG CTCTATTCAA AAAGGTTCGA GCCTCCGTAA ACATGGCCAT GAACAGGAAG 
TCCGTTGCCG AAACCAGCTT AACCAGCAAC CAACCAGGAG GAGCTTCGGC CAAGCCTCAA 
ACTGAAGCAG AATACTTTGC AGCCACCGCC GCCCGACTGG CGGATTCCAA TTCGGTGAGC 
TGTGAGGACC TGCTGGAGTT CTCTGACAAG AAGCCCAAGG GCTGCGAACG AGGCGTGGAC 
TCGGACGAAG TGCGAATCAT GGTAAAAGTG CTCGGCAAAG ATAGCACTCC CAATCGTTGC 
TTGGGTGCCC TGGAGTTCAT CAACTGGGAC GTGCACAAGT CTATTAAGCT CATCAAGTTG 
CAGAACCTGG TTAGCGAGGC AAATCTCAGC CTGGAAGCCT CCTTTGAAGC GCTGCAGCAG 
CACGAATGGG ATCTGCACAC AACGGCCCAC AAACTAAATG GCCTTAAACT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477086.2
CDS	56..4069
Translation	MEYPQIDLYEFLTESELQQYYNAVKNELKITNAAQFKYAADEDLRFIGLSRPEIRRLRKFYEKHFPHSYLSKIKRLLQAPGTMVKREEAPGGGSQVALDGSSASACSSLAAKNGASSPSKVPNNKHIIPADSISVNKQLGTGEFGIVQQGVWSNGNERIQVAIKCLCRERMQSNPMEFLKEAAIMHSIEHENIVRLYGVVLATDSLMLVTELAHLRSLLECLKDSGLRVSFLTIPTLCEFALQICNGMRYLEQKRLIHRDLAARNILVFSKDKVKISDFGLSRALGVGKDYYKTNFNVNLKLPIAWCAPECINYLRFTNASDVWAFGVCLWEMFSYGFQPWAALTGLQILEAIDAPNYQRLEQPDCCPSEYYTLMMKCWQDDAAKRPRFGEIYDQLPDMKPEQLKAVVNCTEPKKDHLLYRQGDIISVLDRNTGTPFWKGVLSTGKTGYFNPSNTVAFLEGLPSSTRDSFSRVSDHRISKRKLRTEMISKPQNDFKHTGHVGIDGATFGDIAFLGSSQNYNHVPKQIVTPYKPSEDIEQTPLLLPPTPTSPDSLQTASGYFPEGANSGGAMGTSMNPTFIPSAEHTPKLIATNGQSSFDFASGSTNPFFPNRGDDELEFGLHNYGADGKSVHSETGWRPTSRSIVDDPHEYHEISDDEIAADKLDFGPSLLDEINSMFGSISAATGSHPKSPGFDHVNNKNEITEMSAKLGQKSGDTNGNKHGHGLLPTLSKKKSSGTVKPISVKDEKILNHAIEIANEISARSMIDLVSDQTPVIHSPKRKFSFRFPHLSNNGSGDKSGGLGTSGSAHTPTHGNASPFPKKKNFTEELQSIPDIQSLIGKEGLEAYNSLIERKALLDIGPSPAATLLRHLDTDEFDLQSLHQSQRPMTLPTRGATQRVRKAELAAGLSRHNDENSNSLEACESPSYMTHGSYKFPEAQPTEQLPEPESPNPIPLPPREGKKQVKTSTKRHVRKYPLIIPANGLQRTLSKLADFGDEAAKSPEISTSSQPQPGRAIEVVAAVRPSGMRRPSRPSEREYENMPTVGKESAHTYQNLDKLTPADAAGLTDTASLQFESIMEADTSKEGILQSPDVTDGFYNFSIQKEHYNKGKDAEFEATQISGLYVNDDELRNLDIESSRRTATPCSSCSALESEHSQPDALPSTESVSEVSRFSSVDNELAGNALFKKVRASVNMAMNRKSVAETSLTSNQPGGASAKPQTEAEYFAATAARLADSNSVSCEDLLEFSDKKPKGCERGVDSDEVRIMVKVLGKDSTPNRCLGALEFINWDVHKSIKLIKLQNLVSEANLSLEASFEALQQHEWDLHTTAHKLNGLKL

Target ORF information:

RefSeq Version	NM_057738.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster activated Cdc42 kinase-like, transcript variant A (Ack-like), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057738.4

ORF Insert Sequence:

ATGGAGTATC CACAAATTGA TTTATACGAA TTCCTCACTG AGTCCGAACT GCAGCAGTAC 
TACAATGCCG TCAAAAACGA ATTGAAAATA ACGAACGCGG CCCAATTTAA GTATGCAGCG 
GACGAGGATT TGCGTTTCAT CGGACTCTCG CGCCCGGAAA TCCGGAGGCT GCGTAAGTTC 
TACGAGAAGC ATTTTCCCCA CAGCTACCTC AGCAAGATCA AGCGTCTGCT GCAGGCGCCT 
GGCACTATGG TTAAGCGGGA AGAGGCGCCC GGCGGCGGAA GTCAGGTGGC GCTGGATGGC 
AGTAGTGCCT CTGCATGCTC CTCTCTGGCC GCCAAGAACG GAGCCAGTTC TCCGAGCAAG 
GTGCCCAACA ACAAGCACAT TATACCGGCG GACTCCATTA GTGTAAACAA ACAGTTGGGA 
ACCGGCGAAT TTGGGATCGT CCAACAGGGA GTCTGGTCCA ATGGCAACGA ACGGATCCAA 
GTGGCTATTA AGTGCCTGTG CCGAGAGCGC ATGCAGTCAA ATCCCATGGA GTTCCTTAAG 
GAAGCGGCCA TCATGCATTC CATCGAGCAC GAGAATATCG TGCGCCTATA TGGCGTTGTC 
CTGGCCACTG ACTCGCTCAT GCTGGTCACT GAGCTCGCCC ATCTGAGATC ACTGTTGGAA 
TGTCTCAAGG ATTCGGGACT GCGAGTCAGT TTCCTCACCA TACCCACGCT CTGTGAGTTC 
GCCCTACAGA TCTGCAATGG AATGCGCTAT TTGGAACAGA AGCGCCTCAT CCACAGAGAC 
CTTGCGGCGC GAAATATTTT AGTGTTCAGC AAGGACAAGG TCAAGATCTC CGACTTTGGC 
CTGTCGCGGG CACTGGGCGT GGGCAAGGAC TACTACAAGA CCAATTTCAA CGTGAATTTA 
AAGCTGCCGA TCGCATGGTG TGCGCCCGAG TGTATCAATT ATCTGCGCTT TACCAACGCC 
TCAGATGTGT GGGCCTTTGG AGTTTGCCTG TGGGAAATGT TCTCCTACGG CTTTCAGCCC 
TGGGCGGCAC TTACCGGCTT GCAGATACTC GAGGCCATTG ATGCACCCAA CTACCAGCGG 
CTTGAACAGC CCGACTGCTG TCCCAGCGAG TATTATACAT TAATGATGAA GTGCTGGCAG 
GATGACGCTG CCAAGCGACC GCGCTTCGGT GAAATCTATG ACCAACTGCC CGATATGAAG 
CCAGAGCAGC TTAAAGCTGT AGTGAATTGC ACAGAACCCA AAAAGGACCA TCTACTTTAT 
CGCCAGGGCG ATATTATTAG CGTTCTAGAC CGCAATACGG GCACACCCTT CTGGAAAGGA 
GTGCTTAGCA CTGGCAAAAC AGGCTACTTC AACCCCTCCA ACACTGTGGC ATTCCTCGAA 
GGTCTGCCCA GCTCCACTCG TGATTCATTC AGCCGGGTGA GTGACCACAG AATCAGCAAA 
CGCAAGCTGC GCACTGAGAT GATATCGAAG CCACAGAACG ACTTCAAGCA CACCGGCCAC 
GTAGGTATTG ACGGAGCAAC ATTTGGAGAC ATCGCATTCC TGGGAAGTTC GCAGAATTAC 
AATCACGTAC CCAAACAGAT AGTGACGCCC TATAAACCCT CGGAGGACAT TGAACAAACA 
CCTCTCCTAC TGCCGCCCAC GCCCACTAGT CCTGACTCTC TTCAGACTGC CAGCGGGTAT 
TTCCCAGAGG GTGCCAACAG CGGAGGCGCC ATGGGCACCT CCATGAACCC CACCTTCATT 
CCATCCGCTG AACACACGCC CAAGCTGATA GCTACCAATG GCCAGAGTTC ATTCGACTTT 
GCCAGCGGGT CTACAAATCC GTTTTTTCCT AACAGAGGAG ACGATGAACT GGAGTTTGGC 
TTGCACAACT ATGGTGCGGA TGGGAAAAGT GTCCATTCGG AGACGGGCTG GCGCCCCACC 
TCTCGAAGTA TTGTAGATGA TCCTCATGAA TACCACGAGA TATCAGATGA TGAAATAGCG 
GCTGACAAGC TGGACTTTGG GCCCTCGCTG CTGGACGAGA TTAACTCGAT GTTTGGATCG 
ATAAGCGCTG CTACAGGCAG TCATCCCAAG TCACCCGGAT TTGATCATGT GAACAATAAA 
AATGAGATTA CGGAAATGTC CGCCAAATTG GGTCAAAAAA GTGGTGACAC CAATGGTAAT 
AAACATGGCC ATGGACTGCT GCCCACGCTC TCGAAAAAGA AATCGTCGGG AACTGTGAAA 
CCTATTTCTG TGAAGGATGA AAAGATTCTC AACCATGCCA TCGAGATAGC CAACGAAATT 
AGTGCTAGGT CGATGATTGA TCTGGTCTCT GATCAGACAC CTGTCATCCA CAGTCCTAAG 
CGCAAATTTA GTTTTAGGTT TCCGCACTTG AGTAATAATG GAAGCGGCGA CAAGTCTGGC 
GGATTAGGAA CCAGCGGCTC TGCCCACACG CCCACCCATG GCAATGCTTC GCCTTTTCCC 
AAGAAAAAGA ATTTCACCGA GGAACTGCAG AGCATTCCGG ATATACAGTC GCTGATCGGC 
AAGGAGGGAC TGGAGGCCTA CAACAGCCTG ATCGAGCGCA AGGCCCTGCT GGACATTGGT 
CCCAGTCCGG CGGCCACCTT GCTCCGCCAC CTGGACACCG ACGAGTTCGA TCTTCAAAGC 
CTTCACCAGT CACAGCGCCC AATGACATTG CCCACTCGGG GTGCCACCCA GCGGGTGCGC 
AAGGCTGAAC TGGCCGCCGG CCTGAGTCGG CACAATGACG AGAACTCGAA TTCGCTGGAG 
GCTTGCGAGA GTCCCAGCTA CATGACCCAC GGCAGCTACA AGTTTCCCGA GGCACAGCCG 
ACAGAACAGC TTCCGGAGCC GGAGTCGCCG AATCCCATAC CCTTGCCGCC GCGCGAGGGC 
AAAAAGCAGG TGAAGACCAG CACCAAGCGG CATGTGCGCA AGTATCCGTT GATAATCCCG 
GCCAACGGTC TGCAACGCAC CCTCAGCAAG CTGGCGGACT TTGGTGACGA GGCTGCCAAA 
TCACCGGAGA TCTCCACATC TTCTCAGCCA CAGCCAGGAC GCGCCATCGA AGTAGTGGCC 
GCTGTCCGGC CGAGTGGCAT GCGACGTCCA TCGCGACCCT CGGAGCGGGA ATACGAGAAC 
ATGCCGACAG TGGGTAAAGA ATCCGCACAC ACCTACCAGA ACTTGGATAA GCTGACACCT 
GCGGATGCTG CCGGATTAAC GGATACAGCT TCGTTGCAGT TCGAGTCCAT CATGGAAGCT 
GACACCAGTA AGGAGGGCAT TCTGCAGTCG CCGGATGTAA CCGACGGATT CTACAACTTT 
TCCATTCAGA AGGAGCACTA CAACAAGGGC AAAGACGCCG AGTTTGAGGC CACTCAGATA 
TCCGGCCTGT ATGTGAACGA TGACGAACTA CGTAACCTGG ACATCGAGAG CAGTCGGCGC 
ACAGCCACTC CATGCAGTAG TTGCAGCGCT TTGGAATCGG AGCACAGCCA ACCAGACGCG 
TTGCCGTCAA CAGAGTCTGT GTCGGAAGTG AGTCGCTTCT CAAGTGTGGA CAATGAGCTG 
GCTGGAAATG CTCTATTCAA AAAGGTTCGA GCCTCCGTAA ACATGGCCAT GAACAGGAAG 
TCCGTTGCCG AAACCAGCTT AACCAGCAAC CAACCAGGAG GAGCTTCGGC CAAGCCTCAA 
ACTGAAGCAG AATACTTTGC AGCCACCGCC GCCCGACTGG CGGATTCCAA TTCGGTGAGC 
TGTGAGGACC TGCTGGAGTT CTCTGACAAG AAGCCCAAGG GCTGCGAACG AGGCGTGGAC 
TCGGACGAAG TGCGAATCAT GGTAAAAGTG CTCGGCAAAG ATAGCACTCC CAATCGTTGC 
TTGGGTGCCC TGGAGTTCAT CAACTGGGAC GTGCACAAGT CTATTAAGCT CATCAAGTTG 
CAGAACCTGG TTAGCGAGGC AAATCTCAGC CTGGAAGCCT CCTTTGAAGC GCTGCAGCAG 
CACGAATGGG ATCTGCACAC AACGGCCCAC AAACTAAATG GCCTTAAACT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ack-like ( NM_057739.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477087.3 Drosophila melanogaster activated Cdc42 kinase-like, transcript variant D (Ack-like), mRNA.

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CloneID	OFa12963
Clone ID Related Accession (Same CDS sequence)	NM_057739.4
Accession Version	NM_057739.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	activated Cdc42 kinase-like, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_057739.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTATC CACAAATTGA TTTATACGAA TTCCTCACTG AGTCCGAACT GCAGCAGTAC 
TACAATGCCG TCAAAAACGA ATTGAAAATA ACGAACGCGG CCCAATTTAA GTATGCAGCG 
GACGAGGATT TGCGTTTCAT CGGACTCTCG CGCCCGGAAA TCCGGAGGCT GCGTAAGTTC 
TACGAGAAGC ATTTTCCCCA CAGCTACCTC AGCAAGATCA AGCGTCTGCT GCAGGCGCCT 
GGCACTATGG TTAAGCGGGA AGAGGCGCCC GGCGGCGGAA GTCAGGTGGC GCTGGATGGC 
AGTAGTGCCT CTGCATGCTC CTCTCTGGCC GCCAAGAACG GAGCCAGTTC TCCGAGCAAG 
GTGCCCAACA ACAAGCACAT TATACCGGCG GACTCCATTA GTGTAAACAA ACAGTTGGGA 
ACCGGCGAAT TTGGGATCGT CCAACAGGGA GTCTGGTCCA ATGGCAACGA ACGGATCCAA 
GTGGCTATTA AGTGCCTGTG CCGAGAGCGC ATGCAGTCAA ATCCCATGGA GTTCCTTAAG 
GAAGCGGCCA TCATGCATTC CATCGAGCAC GAGAATATCG TGCGCCTATA TGGCGTTGTC 
CTGGCCACTG ACTCGCTCAT GCTGGTCACT GAGCTCGCCC ATCTGAGATC ACTGTTGGAA 
TGTCTCAAGG ATTCGGGACT GCGAGTCAGT TTCCTCACCA TACCCACGCT CTGTGAGTTC 
GCCCTACAGA TCTGCAATGG AATGCGCTAT TTGGAACAGA AGCGCCTCAT CCACAGAGAC 
CTTGCGGCGC GAAATATTTT AGTGTTCAGC AAGGACAAGG TCAAGATCTC CGACTTTGGC 
CTGTCGCGGG CACTGGGCGT GGGCAAGGAC TACTACAAGA CCAATTTCAA CGTGAATTTA 
AAGCTGCCGA TCGCATGGTG TGCGCCCGAG TGTATCAATT ATCTGCGCTT TACCAACGCC 
TCAGATGTGT GGGCCTTTGG AGTTTGCCTG TGGGAAATGT TCTCCTACGG CTTTCAGCCC 
TGGGCGGCAC TTACCGGCTT GCAGATACTC GAGGCCATTG ATGCACCCAA CTACCAGCGG 
CTTGAACAGC CCGACTGCTG TCCCAGCGAG TATTATACAT TAATGATGAA GTGCTGGCAG 
GATGACGCTG CCAAGCGACC GCGCTTCGGT GAAATCTATG ACCAACTGCC CGATATGAAG 
CCAGAGCAGC TTAAAGCTGT AGTGAATTGC ACAGAACCCA AAAAGGACCA TCTACTTTAT 
CGCCAGGGCG ATATTATTAG CGTTCTAGAC CGCAATACGG GCACACCCTT CTGGAAAGGA 
GTGCTTAGCA CTGGCAAAAC AGGCTACTTC AACCCCTCCA ACACTGTGGC ATTCCTCGAA 
GGTCTGCCCA GCTCCACTCG TGATTCATTC AGCCGGGTGA GTGACCACAG AATCAGCAAA 
CGCAAGCTGC GCACTGAGAT GATATCGAAG CCACAGAACG ACTTCAAGCA CACCGGCCAC 
GTAGGTATTG ACGGAGCAAC ATTTGGAGAC ATCGCATTCC TGGGAAGTTC GCAGAATTAC 
AATCACGTAC CCAAACAGAT AGTGACGCCC TATAAACCCT CGGAGGACAT TGAACAAACA 
CCTCTCCTAC TGCCGCCCAC GCCCACTAGT CCTGACTCTC TTCAGACTGC CAGCGGGTAT 
TTCCCAGAGG GTGCCAACAG CGGAGGCGCC ATGGGCACCT CCATGAACCC CACCTTCATT 
CCATCCGCTG AACACACGCC CAAGCTGATA GCTACCAATG GCCAGAGTTC ATTCGACTTT 
GCCAGCGGGT CTACAAATCC GTTTTTTCCT AACAGAGGAG ACGATGAACT GGAGTTTGGC 
TTGCACAACT ATGGTGCGGA TGGGAAAAGT GTCCATTCGG AGACGGGCTG GCGCCCCACC 
TCTCGAAGTA TTGTAGATGA TCCTCATGAA TACCACGAGA TATCAGATGA TGAAATAGCG 
GCTGACAAGC TGGACTTTGG GCCCTCGCTG CTGGACGAGA TTAACTCGAT GTTTGGATCG 
ATAAGCGCTG CTACAGGCAG TCATCCCAAG TCACCCGGAT TTGATCATGT GAACAATAAA 
AATGAGATTA CGGAAATGTC CGCCAAATTG GGTCAAAAAA GTGGTGACAC CAATGGTAAT 
AAACATGGCC ATGGACTGCT GCCCACGCTC TCGAAAAAGA AATCGTCGGG AACTGTGAAA 
CCTATTTCTG TGAAGGATGA AAAGATTCTC AACCATGCCA TCGAGATAGC CAACGAAATT 
AGTGCTAGGT CGATGATTGA TCTGGTCTCT GATCAGACAC CTGTCATCCA CAGTCCTAAG 
CGCAAATTTA GTTTTAGGTT TCCGCACTTG AGTAATAATG GAAGCGGCGA CAAGTCTGGC 
GGATTAGGAA CCAGCGGCTC TGCCCACACG CCCACCCATG GCAATGCTTC GCCTTTTCCC 
AAGAAAAAGA ATTTCACCGA GGAACTGCAG AGCATTCCGG ATATACAGCA GTCGCTGATC 
GGCAAGGAGG GACTGGAGGC CTACAACAGC CTGATCGAGC GCAAGGCCCT GCTGGACATT 
GGTCCCAGTC CGGCGGCCAC CTTGCTCCGC CACCTGGACA CCGACGAGTT CGATCTTCAA 
AGCCTTCACC AGTCACAGCG CCCAATGACA TTGCCCACTC GGGGTGCCAC CCAGCGGGTG 
CGCAAGGCTG AACTGGCCGC CGGCCTGAGT CGGCACAATG ACGAGAACTC GAATTCGCTG 
GAGGCTTGCG AGAGTCCCAG CTACATGACC CACGGCAGCT ACAAGTTTCC CGAGGCACAG 
CCGACAGAAC AGCTTCCGGA GCCGGAGTCG CCGAATCCCA TACCCTTGCC GCCGCGCGAG 
GGCAAAAAGC AGGTGAAGAC CAGCACCAAG CGGCATGTGC GCAAGTATCC GTTGATAATC 
CCGGCCAACG GTCTGCAACG CACCCTCAGC AAGCTGGCGG ACTTTGGTGA CGAGGCTGCC 
AAATCACCGG AGATCTCCAC ATCTTCTCAG CCACAGCCAG GACGCGCCAT CGAAGTAGTG 
GCCGCTGTCC GGCCGAGTGG CATGCGACGT CCATCGCGAC CCTCGGAGCG GGAATACGAG 
AACATGCCGA CAGTGGGTAA AGAATCCGCA CACACCTACC AGAACTTGGA TAAGCTGACA 
CCTGCGGATG CTGCCGGATT AACGGATACA GCTTCGTTGC AGTTCGAGTC CATCATGGAA 
GCTGACACCA GTAAGGAGGG CATTCTGCAG TCGCCGGATG TAACCGACGG ATTCTACAAC 
TTTTCCATTC AGAAGGAGCA CTACAACAAG GGCAAAGACG CCGAGTTTGA GGCCACTCAG 
ATATCCGGCC TGTATGTGAA CGATGACGAA CTACGTAACC TGGACATCGA GAGCAGTCGG 
CGCACAGCCA CTCCATGCAG TAGTTGCAGC GCTTTGGAAT CGGAGCACAG CCAACCAGAC 
GCGTTGCCGT CAACAGAGTC TGTGTCGGAA GTGAGTCGCT TCTCAAGTGT GGACAATGAG 
CTGGCTGGAA ATGCTCTATT CAAAAAGGTT CGAGCCTCCG TAAACATGGC CATGAACAGG 
AAGTCCGTTG CCGAAACCAG CTTAACCAGC AACCAACCAG GAGGAGCTTC GGCCAAGCCT 
CAAACTGAAG CAGAATACTT TGCAGCCACC GCCGCCCGAC TGGCGGATTC CAATTCGGTG 
AGCTGTGAGG ACCTGCTGGA GTTCTCTGAC AAGAAGCCCA AGGGCTGCGA ACGAGGCGTG 
GACTCGGACG AAGTGCGAAT CATGGTAAAA GTGCTCGGCA AAGATAGCAC TCCCAATCGT 
TGCTTGGGTG CCCTGGAGTT CATCAACTGG GACGTGCACA AGTCTATTAA GCTCATCAAG 
TTGCAGAACC TGGTTAGCGA GGCAAATCTC AGCCTGGAAG CCTCCTTTGA AGCGCTGCAG 
CAGCACGAAT GGGATCTGCA CACAACGGCC CACAAACTAA ATGGCCTTAA ACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477087.3
CDS	56..4072
Translation	MEYPQIDLYEFLTESELQQYYNAVKNELKITNAAQFKYAADEDLRFIGLSRPEIRRLRKFYEKHFPHSYLSKIKRLLQAPGTMVKREEAPGGGSQVALDGSSASACSSLAAKNGASSPSKVPNNKHIIPADSISVNKQLGTGEFGIVQQGVWSNGNERIQVAIKCLCRERMQSNPMEFLKEAAIMHSIEHENIVRLYGVVLATDSLMLVTELAHLRSLLECLKDSGLRVSFLTIPTLCEFALQICNGMRYLEQKRLIHRDLAARNILVFSKDKVKISDFGLSRALGVGKDYYKTNFNVNLKLPIAWCAPECINYLRFTNASDVWAFGVCLWEMFSYGFQPWAALTGLQILEAIDAPNYQRLEQPDCCPSEYYTLMMKCWQDDAAKRPRFGEIYDQLPDMKPEQLKAVVNCTEPKKDHLLYRQGDIISVLDRNTGTPFWKGVLSTGKTGYFNPSNTVAFLEGLPSSTRDSFSRVSDHRISKRKLRTEMISKPQNDFKHTGHVGIDGATFGDIAFLGSSQNYNHVPKQIVTPYKPSEDIEQTPLLLPPTPTSPDSLQTASGYFPEGANSGGAMGTSMNPTFIPSAEHTPKLIATNGQSSFDFASGSTNPFFPNRGDDELEFGLHNYGADGKSVHSETGWRPTSRSIVDDPHEYHEISDDEIAADKLDFGPSLLDEINSMFGSISAATGSHPKSPGFDHVNNKNEITEMSAKLGQKSGDTNGNKHGHGLLPTLSKKKSSGTVKPISVKDEKILNHAIEIANEISARSMIDLVSDQTPVIHSPKRKFSFRFPHLSNNGSGDKSGGLGTSGSAHTPTHGNASPFPKKKNFTEELQSIPDIQQSLIGKEGLEAYNSLIERKALLDIGPSPAATLLRHLDTDEFDLQSLHQSQRPMTLPTRGATQRVRKAELAAGLSRHNDENSNSLEACESPSYMTHGSYKFPEAQPTEQLPEPESPNPIPLPPREGKKQVKTSTKRHVRKYPLIIPANGLQRTLSKLADFGDEAAKSPEISTSSQPQPGRAIEVVAAVRPSGMRRPSRPSEREYENMPTVGKESAHTYQNLDKLTPADAAGLTDTASLQFESIMEADTSKEGILQSPDVTDGFYNFSIQKEHYNKGKDAEFEATQISGLYVNDDELRNLDIESSRRTATPCSSCSALESEHSQPDALPSTESVSEVSRFSSVDNELAGNALFKKVRASVNMAMNRKSVAETSLTSNQPGGASAKPQTEAEYFAATAARLADSNSVSCEDLLEFSDKKPKGCERGVDSDEVRIMVKVLGKDSTPNRCLGALEFINWDVHKSIKLIKLQNLVSEANLSLEASFEALQQHEWDLHTTAHKLNGLKL

Target ORF information:

RefSeq Version	NM_057739.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster activated Cdc42 kinase-like, transcript variant D (Ack-like), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057739.4

ORF Insert Sequence:

ATGGAGTATC CACAAATTGA TTTATACGAA TTCCTCACTG AGTCCGAACT GCAGCAGTAC 
TACAATGCCG TCAAAAACGA ATTGAAAATA ACGAACGCGG CCCAATTTAA GTATGCAGCG 
GACGAGGATT TGCGTTTCAT CGGACTCTCG CGCCCGGAAA TCCGGAGGCT GCGTAAGTTC 
TACGAGAAGC ATTTTCCCCA CAGCTACCTC AGCAAGATCA AGCGTCTGCT GCAGGCGCCT 
GGCACTATGG TTAAGCGGGA AGAGGCGCCC GGCGGCGGAA GTCAGGTGGC GCTGGATGGC 
AGTAGTGCCT CTGCATGCTC CTCTCTGGCC GCCAAGAACG GAGCCAGTTC TCCGAGCAAG 
GTGCCCAACA ACAAGCACAT TATACCGGCG GACTCCATTA GTGTAAACAA ACAGTTGGGA 
ACCGGCGAAT TTGGGATCGT CCAACAGGGA GTCTGGTCCA ATGGCAACGA ACGGATCCAA 
GTGGCTATTA AGTGCCTGTG CCGAGAGCGC ATGCAGTCAA ATCCCATGGA GTTCCTTAAG 
GAAGCGGCCA TCATGCATTC CATCGAGCAC GAGAATATCG TGCGCCTATA TGGCGTTGTC 
CTGGCCACTG ACTCGCTCAT GCTGGTCACT GAGCTCGCCC ATCTGAGATC ACTGTTGGAA 
TGTCTCAAGG ATTCGGGACT GCGAGTCAGT TTCCTCACCA TACCCACGCT CTGTGAGTTC 
GCCCTACAGA TCTGCAATGG AATGCGCTAT TTGGAACAGA AGCGCCTCAT CCACAGAGAC 
CTTGCGGCGC GAAATATTTT AGTGTTCAGC AAGGACAAGG TCAAGATCTC CGACTTTGGC 
CTGTCGCGGG CACTGGGCGT GGGCAAGGAC TACTACAAGA CCAATTTCAA CGTGAATTTA 
AAGCTGCCGA TCGCATGGTG TGCGCCCGAG TGTATCAATT ATCTGCGCTT TACCAACGCC 
TCAGATGTGT GGGCCTTTGG AGTTTGCCTG TGGGAAATGT TCTCCTACGG CTTTCAGCCC 
TGGGCGGCAC TTACCGGCTT GCAGATACTC GAGGCCATTG ATGCACCCAA CTACCAGCGG 
CTTGAACAGC CCGACTGCTG TCCCAGCGAG TATTATACAT TAATGATGAA GTGCTGGCAG 
GATGACGCTG CCAAGCGACC GCGCTTCGGT GAAATCTATG ACCAACTGCC CGATATGAAG 
CCAGAGCAGC TTAAAGCTGT AGTGAATTGC ACAGAACCCA AAAAGGACCA TCTACTTTAT 
CGCCAGGGCG ATATTATTAG CGTTCTAGAC CGCAATACGG GCACACCCTT CTGGAAAGGA 
GTGCTTAGCA CTGGCAAAAC AGGCTACTTC AACCCCTCCA ACACTGTGGC ATTCCTCGAA 
GGTCTGCCCA GCTCCACTCG TGATTCATTC AGCCGGGTGA GTGACCACAG AATCAGCAAA 
CGCAAGCTGC GCACTGAGAT GATATCGAAG CCACAGAACG ACTTCAAGCA CACCGGCCAC 
GTAGGTATTG ACGGAGCAAC ATTTGGAGAC ATCGCATTCC TGGGAAGTTC GCAGAATTAC 
AATCACGTAC CCAAACAGAT AGTGACGCCC TATAAACCCT CGGAGGACAT TGAACAAACA 
CCTCTCCTAC TGCCGCCCAC GCCCACTAGT CCTGACTCTC TTCAGACTGC CAGCGGGTAT 
TTCCCAGAGG GTGCCAACAG CGGAGGCGCC ATGGGCACCT CCATGAACCC CACCTTCATT 
CCATCCGCTG AACACACGCC CAAGCTGATA GCTACCAATG GCCAGAGTTC ATTCGACTTT 
GCCAGCGGGT CTACAAATCC GTTTTTTCCT AACAGAGGAG ACGATGAACT GGAGTTTGGC 
TTGCACAACT ATGGTGCGGA TGGGAAAAGT GTCCATTCGG AGACGGGCTG GCGCCCCACC 
TCTCGAAGTA TTGTAGATGA TCCTCATGAA TACCACGAGA TATCAGATGA TGAAATAGCG 
GCTGACAAGC TGGACTTTGG GCCCTCGCTG CTGGACGAGA TTAACTCGAT GTTTGGATCG 
ATAAGCGCTG CTACAGGCAG TCATCCCAAG TCACCCGGAT TTGATCATGT GAACAATAAA 
AATGAGATTA CGGAAATGTC CGCCAAATTG GGTCAAAAAA GTGGTGACAC CAATGGTAAT 
AAACATGGCC ATGGACTGCT GCCCACGCTC TCGAAAAAGA AATCGTCGGG AACTGTGAAA 
CCTATTTCTG TGAAGGATGA AAAGATTCTC AACCATGCCA TCGAGATAGC CAACGAAATT 
AGTGCTAGGT CGATGATTGA TCTGGTCTCT GATCAGACAC CTGTCATCCA CAGTCCTAAG 
CGCAAATTTA GTTTTAGGTT TCCGCACTTG AGTAATAATG GAAGCGGCGA CAAGTCTGGC 
GGATTAGGAA CCAGCGGCTC TGCCCACACG CCCACCCATG GCAATGCTTC GCCTTTTCCC 
AAGAAAAAGA ATTTCACCGA GGAACTGCAG AGCATTCCGG ATATACAGCA GTCGCTGATC 
GGCAAGGAGG GACTGGAGGC CTACAACAGC CTGATCGAGC GCAAGGCCCT GCTGGACATT 
GGTCCCAGTC CGGCGGCCAC CTTGCTCCGC CACCTGGACA CCGACGAGTT CGATCTTCAA 
AGCCTTCACC AGTCACAGCG CCCAATGACA TTGCCCACTC GGGGTGCCAC CCAGCGGGTG 
CGCAAGGCTG AACTGGCCGC CGGCCTGAGT CGGCACAATG ACGAGAACTC GAATTCGCTG 
GAGGCTTGCG AGAGTCCCAG CTACATGACC CACGGCAGCT ACAAGTTTCC CGAGGCACAG 
CCGACAGAAC AGCTTCCGGA GCCGGAGTCG CCGAATCCCA TACCCTTGCC GCCGCGCGAG 
GGCAAAAAGC AGGTGAAGAC CAGCACCAAG CGGCATGTGC GCAAGTATCC GTTGATAATC 
CCGGCCAACG GTCTGCAACG CACCCTCAGC AAGCTGGCGG ACTTTGGTGA CGAGGCTGCC 
AAATCACCGG AGATCTCCAC ATCTTCTCAG CCACAGCCAG GACGCGCCAT CGAAGTAGTG 
GCCGCTGTCC GGCCGAGTGG CATGCGACGT CCATCGCGAC CCTCGGAGCG GGAATACGAG 
AACATGCCGA CAGTGGGTAA AGAATCCGCA CACACCTACC AGAACTTGGA TAAGCTGACA 
CCTGCGGATG CTGCCGGATT AACGGATACA GCTTCGTTGC AGTTCGAGTC CATCATGGAA 
GCTGACACCA GTAAGGAGGG CATTCTGCAG TCGCCGGATG TAACCGACGG ATTCTACAAC 
TTTTCCATTC AGAAGGAGCA CTACAACAAG GGCAAAGACG CCGAGTTTGA GGCCACTCAG 
ATATCCGGCC TGTATGTGAA CGATGACGAA CTACGTAACC TGGACATCGA GAGCAGTCGG 
CGCACAGCCA CTCCATGCAG TAGTTGCAGC GCTTTGGAAT CGGAGCACAG CCAACCAGAC 
GCGTTGCCGT CAACAGAGTC TGTGTCGGAA GTGAGTCGCT TCTCAAGTGT GGACAATGAG 
CTGGCTGGAA ATGCTCTATT CAAAAAGGTT CGAGCCTCCG TAAACATGGC CATGAACAGG 
AAGTCCGTTG CCGAAACCAG CTTAACCAGC AACCAACCAG GAGGAGCTTC GGCCAAGCCT 
CAAACTGAAG CAGAATACTT TGCAGCCACC GCCGCCCGAC TGGCGGATTC CAATTCGGTG 
AGCTGTGAGG ACCTGCTGGA GTTCTCTGAC AAGAAGCCCA AGGGCTGCGA ACGAGGCGTG 
GACTCGGACG AAGTGCGAAT CATGGTAAAA GTGCTCGGCA AAGATAGCAC TCCCAATCGT 
TGCTTGGGTG CCCTGGAGTT CATCAACTGG GACGTGCACA AGTCTATTAA GCTCATCAAG 
TTGCAGAACC TGGTTAGCGA GGCAAATCTC AGCCTGGAAG CCTCCTTTGA AGCGCTGCAG 
CAGCACGAAT GGGATCTGCA CACAACGGCC CACAAACTAA ATGGCCTTAA ACTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ack-like cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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