CG8545 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG8545
Entrez Gene ID	36365
Full Name	CG8545 gene product from transcript CG8545-RA
Synonyms	Dmel\CG8545,Nol1Nt
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 49A7-49A7

mRNA and Protein(s)

mRNA	Protein	Name
NM_136942.3	NP_610786.2	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG8545 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG8545 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09758	NM_136942.3 Latest version!	Drosophila melanogaster uncharacterized protein (CG8545), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG8545 ( NM_136942.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610786.2 Drosophila melanogaster uncharacterized protein (CG8545), mRNA.

Price & Availability↑

CloneID	OFa09758
Clone ID Related Accession (Same CDS sequence)	NM_136942.3
Accession Version	NM_136942.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136942.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTCGGA AGGCTGAATA CAGTGAGAAA CCGAAAAAGG GACCTGGGCG CAAGGCGCGC 
AAGCAAGGAC CGCCTGTTTT TCGCAAACAA TCGTTTGCTC CCATGGAAGA GGAGGACAAG 
AAGCTTTCCC ACCGACAGAA GCAGAGGTTC GTTAAGCGAG AGCAAAAGAA AGTAGTCCAG 
AAGGCCAAAC TACAGGAGAA GAGGGCCAAG GGAAAGTTAC CACAACCGGC TAAGCGGAAG 
ACGGCCTACA ACAGCGACAG CGATCCTGAG GATAATGAAG AGGAACCACA GGTTGCATCC 
TCCGATGAGG AGGTGCCTCA ACTGGTGCCG GCTCCGAAGG GCCAGAAAGC TACAGCTCCC 
AAGGGTTTTA CAGATGATAA TGATGCCTGG CTGAAGCCCA AGAAGCAACA GAAGCAGCCA 
GAAACGGAGT CCGAGGAGGA GGAGGAGGAG GATGAGGAGT CGGAGGAGGA CTCCGAAGAT 
GAGTTGGGAG AAGAATGTGA AGAGGAAGAG GAGGGAGACG ATGAGGAAGG ATTGGAATCT 
GATGATGATA CCGCTCAGGT TGGCAAGCTG GCCGATCTAT CCGACGATGA TGATGCCAAC 
TCCGACGATG ACTTTGACAT ATCTGGCGAG GAAGATGGTG CTCAGGCAGA CAGTGATGAA 
GAGGAGGATG AGGACGACGA CGATGATGAT GATGATGATG ATGATGACAA GCTGCCCATC 
GAGCGGGCTA ACAAGAAGTT GAAAAAGCGC GAGGCAGAGG AAGCCCAACT GGCCGACGAC 
GAAATGTTGG AAACCGTTGA CAAGCAGGAT GTTTTCCAGC TACCCGTCGA AGGCGAAGAA 
ACCGAGAAGG ACCTAACCCT GCAGGAAGTG CAACAGCGCA TAAAGGACGT CAGTCTGGTT 
CTTTCCGACT TCAAAAGGTA TCGTCAAGCG GATAGGTCTC GCGGGGAGTA CATCGATTTG 
CTGCGCAGGG ATCTGTGTTT GTATTACAGC TACAACGAAT TTTTGATGGA GAAGCTGATG 
GATATGTTGC CGCTGACAGA GCTAATGGAA TATCTGGAGG CCTCAGAGAT TGCTCGTCCC 
CTAACCATAC GCACCAATAC TCTAAAAACA CGTCGTAGGG ATTTGGCCGG AGCTCTGATC 
AATCGTGGCG TCAACTTGGA TCCCCTTGGT AAATGGACGA AAGTTGGATT GGTTGTCTTT 
AACTCACAGG TGCCACTGGG TGCAACTCCA GAGTATCTGG CTGGCCACTA CATGATCCAG 
GGCGCCTCTT CGCTGTTGCC TGTAATGGCA CTCGCCCCGC AAGAGAACGA ACGCATCCTG 
GACATGTGCT CCGCCCCAGG TGGAAAGGGA TCCCACATCG CAAGCATCAT GAAGAACACT 
GGTGTGCTCT TCGCCAACGA TTCTAACAGG GATCGCATCA AGGCCATTGT TGCCAATTTC 
CATCGCCTGG GAATTGTAAA TGCAGTGGTC AGCTGCGAGG ATGGCACTAA GTTCCGGAAT 
ATTATGACTG GCTTTGACCG TGTCCTGCTG GATGCTCCGT GCACTGGAAC TGGCGTCGTC 
TCTAAGGATC CCAGTGTGAA GACCACCAAG TCGGAAGTAG ATGTACAAAG GTGCTACAAC 
CTGCAGAGGA AGCTTCTGCT GACTGCCATA GATTGTACGG ATGCGAAGTC ATCAACTGGC 
GGCTACATTG TATACTCAAC TTGTTCGGTG TTGCCCGAGG AGAACGAATG GGTCATCGAC 
TATGCACTGA AGAAGCGAAA TGTCAAACTT GTGCCCACTG GTCTGGACTT CGGCGTGGAG 
GGCTTTACCA AATTCCGTCA GCATCGGTTC CATCCTAGCT TAAACCTTAC CAAGCGCTAC 
TATCCGCACA CCCACAACAT GGACGGGTTC TACGTGGCCA AGCTCAAGAA ATTCTCCAAC 
ACCATACCCA TAACTAAGGA GCAGCAGGAG GAGGATGAAA AGCAGCTGGA TGAGGCCATC 
GAAGCGGACG CCACTACAGA AGCGGCTGCG GAGTCTGAGG AGGTGGAGGA GGAGGTAGCG 
TCAAAGGAGA AGGGTCCCCG AAAACTGCTT GGAAAGCGCG CCGGAAAACC CAGTTTCACC 
GAAATCGAAC AGGAACTGAA GAAAAAGAAG CGGGAAGAGA GCAAGACCAA GTATGTGGCC 
AAGGTGTTTG AGCAGCCTGT CAAAAAGCCA AAGCCAGAAA AGCCAGCAGA GAAGAAAAAT 
GAGAAATCCC AGGCACCGGA CAAGCAAACA AATTCAAGCG AGTCACCTTC CAAGTCTGCA 
CCTAAACAAA AACAAACCAA TGGGAAAACC ACTGTTTCCA ATTCAGATGC GAAATCATTC 
AATAAAAAGA ACAAAGGATC GCAACCCAAG GAAAATGGAG CCGGATCACC AGCTAAACCA 
GATGACAATA AGCCAACAAC CAATGGCACA AACTCCCCTA GCAAATCAGC AATTAAGGAA 
ACGAAAACGA AGCCACAGCC GAAAGGAAAA CAGTCGCCCA AGGGAAACGC CAAACTGGCC 
AAGGCGCCGC GAGTGGATAT CGACGATGTG CCCATTCTCG AGGGAAAACC CATCAAGAAG 
CAGAACAAGC TGAAGCAAAA ATCCAAACAG ATCGGCCAGC TGAAAAAGTC CACGGCGGGT 
GCGAATGCGG GAAAGAAGCA AAAGTTCAAG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610786.2
CDS	103..2778
Translation	MGRKAEYSEKPKKGPGRKARKQGPPVFRKQSFAPMEEEDKKLSHRQKQRFVKREQKKVVQKAKLQEKRAKGKLPQPAKRKTAYNSDSDPEDNEEEPQVASSDEEVPQLVPAPKGQKATAPKGFTDDNDAWLKPKKQQKQPETESEEEEEEDEESEEDSEDELGEECEEEEEGDDEEGLESDDDTAQVGKLADLSDDDDANSDDDFDISGEEDGAQADSDEEEDEDDDDDDDDDDDDKLPIERANKKLKKREAEEAQLADDEMLETVDKQDVFQLPVEGEETEKDLTLQEVQQRIKDVSLVLSDFKRYRQADRSRGEYIDLLRRDLCLYYSYNEFLMEKLMDMLPLTELMEYLEASEIARPLTIRTNTLKTRRRDLAGALINRGVNLDPLGKWTKVGLVVFNSQVPLGATPEYLAGHYMIQGASSLLPVMALAPQENERILDMCSAPGGKGSHIASIMKNTGVLFANDSNRDRIKAIVANFHRLGIVNAVVSCEDGTKFRNIMTGFDRVLLDAPCTGTGVVSKDPSVKTTKSEVDVQRCYNLQRKLLLTAIDCTDAKSSTGGYIVYSTCSVLPEENEWVIDYALKKRNVKLVPTGLDFGVEGFTKFRQHRFHPSLNLTKRYYPHTHNMDGFYVAKLKKFSNTIPITKEQQEEDEKQLDEAIEADATTEAAAESEEVEEEVASKEKGPRKLLGKRAGKPSFTEIEQELKKKKREESKTKYVAKVFEQPVKKPKPEKPAEKKNEKSQAPDKQTNSSESPSKSAPKQKQTNGKTTVSNSDAKSFNKKNKGSQPKENGAGSPAKPDDNKPTTNGTNSPSKSAIKETKTKPQPKGKQSPKGNAKLAKAPRVDIDDVPILEGKPIKKQNKLKQKSKQIGQLKKSTAGANAGKKQKFKK

Target ORF information:

RefSeq Version	NM_136942.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein (CG8545), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136942.3

ORF Insert Sequence:

ATGGGTCGGA AGGCTGAATA CAGTGAGAAA CCGAAAAAGG GACCTGGGCG CAAGGCGCGC 
AAGCAAGGAC CGCCTGTTTT TCGCAAACAA TCGTTTGCTC CCATGGAAGA GGAGGACAAG 
AAGCTTTCCC ACCGACAGAA GCAGAGGTTC GTTAAGCGAG AGCAAAAGAA AGTAGTCCAG 
AAGGCCAAAC TACAGGAGAA GAGGGCCAAG GGAAAGTTAC CACAACCGGC TAAGCGGAAG 
ACGGCCTACA ACAGCGACAG CGATCCTGAG GATAATGAAG AGGAACCACA GGTTGCATCC 
TCCGATGAGG AGGTGCCTCA ACTGGTGCCG GCTCCGAAGG GCCAGAAAGC TACAGCTCCC 
AAGGGTTTTA CAGATGATAA TGATGCCTGG CTGAAGCCCA AGAAGCAACA GAAGCAGCCA 
GAAACGGAGT CCGAGGAGGA GGAGGAGGAG GATGAGGAGT CGGAGGAGGA CTCCGAAGAT 
GAGTTGGGAG AAGAATGTGA AGAGGAAGAG GAGGGAGACG ATGAGGAAGG ATTGGAATCT 
GATGATGATA CCGCTCAGGT TGGCAAGCTG GCCGATCTAT CCGACGATGA TGATGCCAAC 
TCCGACGATG ACTTTGACAT ATCTGGCGAG GAAGATGGTG CTCAGGCAGA CAGTGATGAA 
GAGGAGGATG AGGACGACGA CGATGATGAT GATGATGATG ATGATGACAA GCTGCCCATC 
GAGCGGGCTA ACAAGAAGTT GAAAAAGCGC GAGGCAGAGG AAGCCCAACT GGCCGACGAC 
GAAATGTTGG AAACCGTTGA CAAGCAGGAT GTTTTCCAGC TACCCGTCGA AGGCGAAGAA 
ACCGAGAAGG ACCTAACCCT GCAGGAAGTG CAACAGCGCA TAAAGGACGT CAGTCTGGTT 
CTTTCCGACT TCAAAAGGTA TCGTCAAGCG GATAGGTCTC GCGGGGAGTA CATCGATTTG 
CTGCGCAGGG ATCTGTGTTT GTATTACAGC TACAACGAAT TTTTGATGGA GAAGCTGATG 
GATATGTTGC CGCTGACAGA GCTAATGGAA TATCTGGAGG CCTCAGAGAT TGCTCGTCCC 
CTAACCATAC GCACCAATAC TCTAAAAACA CGTCGTAGGG ATTTGGCCGG AGCTCTGATC 
AATCGTGGCG TCAACTTGGA TCCCCTTGGT AAATGGACGA AAGTTGGATT GGTTGTCTTT 
AACTCACAGG TGCCACTGGG TGCAACTCCA GAGTATCTGG CTGGCCACTA CATGATCCAG 
GGCGCCTCTT CGCTGTTGCC TGTAATGGCA CTCGCCCCGC AAGAGAACGA ACGCATCCTG 
GACATGTGCT CCGCCCCAGG TGGAAAGGGA TCCCACATCG CAAGCATCAT GAAGAACACT 
GGTGTGCTCT TCGCCAACGA TTCTAACAGG GATCGCATCA AGGCCATTGT TGCCAATTTC 
CATCGCCTGG GAATTGTAAA TGCAGTGGTC AGCTGCGAGG ATGGCACTAA GTTCCGGAAT 
ATTATGACTG GCTTTGACCG TGTCCTGCTG GATGCTCCGT GCACTGGAAC TGGCGTCGTC 
TCTAAGGATC CCAGTGTGAA GACCACCAAG TCGGAAGTAG ATGTACAAAG GTGCTACAAC 
CTGCAGAGGA AGCTTCTGCT GACTGCCATA GATTGTACGG ATGCGAAGTC ATCAACTGGC 
GGCTACATTG TATACTCAAC TTGTTCGGTG TTGCCCGAGG AGAACGAATG GGTCATCGAC 
TATGCACTGA AGAAGCGAAA TGTCAAACTT GTGCCCACTG GTCTGGACTT CGGCGTGGAG 
GGCTTTACCA AATTCCGTCA GCATCGGTTC CATCCTAGCT TAAACCTTAC CAAGCGCTAC 
TATCCGCACA CCCACAACAT GGACGGGTTC TACGTGGCCA AGCTCAAGAA ATTCTCCAAC 
ACCATACCCA TAACTAAGGA GCAGCAGGAG GAGGATGAAA AGCAGCTGGA TGAGGCCATC 
GAAGCGGACG CCACTACAGA AGCGGCTGCG GAGTCTGAGG AGGTGGAGGA GGAGGTAGCG 
TCAAAGGAGA AGGGTCCCCG AAAACTGCTT GGAAAGCGCG CCGGAAAACC CAGTTTCACC 
GAAATCGAAC AGGAACTGAA GAAAAAGAAG CGGGAAGAGA GCAAGACCAA GTATGTGGCC 
AAGGTGTTTG AGCAGCCTGT CAAAAAGCCA AAGCCAGAAA AGCCAGCAGA GAAGAAAAAT 
GAGAAATCCC AGGCACCGGA CAAGCAAACA AATTCAAGCG AGTCACCTTC CAAGTCTGCA 
CCTAAACAAA AACAAACCAA TGGGAAAACC ACTGTTTCCA ATTCAGATGC GAAATCATTC 
AATAAAAAGA ACAAAGGATC GCAACCCAAG GAAAATGGAG CCGGATCACC AGCTAAACCA 
GATGACAATA AGCCAACAAC CAATGGCACA AACTCCCCTA GCAAATCAGC AATTAAGGAA 
ACGAAAACGA AGCCACAGCC GAAAGGAAAA CAGTCGCCCA AGGGAAACGC CAAACTGGCC 
AAGGCGCCGC GAGTGGATAT CGACGATGTG CCCATTCTCG AGGGAAAACC CATCAAGAAG 
CAGAACAAGC TGAAGCAAAA ATCCAAACAG ATCGGCCAGC TGAAAAAGTC CACGGCGGGT 
GCGAATGCGG GAAAGAAGCA AAAGTTCAAG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG8545 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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