Drep3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Drep3
Entrez Gene ID	36292
Full Name	DNA fragmentation factor-related protein 3
Synonyms	BcDNA:AT08574,CG13187,CG8364,DREP-3,Dmel\CG8364,Drep-3,Rep3,drep-3,drep3
General protein information	Preferred Names DNA fragmentation factor-related protein 3 Names CG8364 gene product from transcript CG8364-RA CG8364-PA CG8364-PB Drep3-PA Drep3-PB Rep3
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 48D8-48D8

mRNA and Protein(s)

mRNA	Protein	Name
NM_136878.3	NP_610722.2	DNA fragmentation factor-related protein 3, isoform B
NM_136879.3	NP_610723.2	DNA fragmentation factor-related protein 3, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that the basic surface side of Drep2 CIDE is involved in the interaction with the acidic surface side of Drep1 CIDE and Drep3 CIDE.
Title: General interaction mode of CIDE:CIDE complex revealed by a mutation study of the Drep2 CIDE domain.

Drep2 is a novel nuclease whose activity is inhibited by Drep3 through a tight interaction with the CIDE domain.
Title: Dual apoptotic DNA fragmentation system in the fly: Drep2 is a novel nuclease of which activity is inhibited by Drep3.

CIDE domains of Drep2 and Drep3 were purified in Escherichia coli, after which they formed a stable complex in vitro and were crystallized by the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to a resolution of 5.8 A.
Title: Crystallization and preliminary X-ray crystallographic studies of the CIDE-domain complex between Drep2 and Drep3 from Drosophila melanogaster.

The following Drep3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Drep3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09678	NM_136878.3 Latest version!	Drosophila melanogaster DNA fragmentation factor-related protein 3 (Drep3), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa09678	NM_136879.3 Latest version!	Drosophila melanogaster DNA fragmentation factor-related protein 3 (Drep3), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Drep3 ( NM_136878.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610722.2 Drosophila melanogaster DNA fragmentation factor-related protein 3 (Drep3), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa09678
Clone ID Related Accession (Same CDS sequence)	NM_136878.3 , NM_136879.3
Accession Version	NM_136878.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	DNA fragmentation factor-related protein 3, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136878.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGCAA TGAATGCGGA TGAGACAAAG CTGGCTGGAA TGCCTCAAGG AGCTGAGGAG 
GAGCAGGAAC CGGAGAGGGA GCAGAAGAAG GAGAGCGATG GAGCAGCAGC TGCAGCAGGA 
GTACAGTGTG ATCCTGATAG CAGAATAGTG GCTCCGCCGC CCAGGCAGCG GACTCTGACA 
AGGACGGCCG AACTGGACGC GGACTGCGAG GACATCGAGT TAGATGCCGA CGATGGTTTG 
GACGATGCGG CGGATAGCAT CACCTTGGAG TTGGCCCTAT CGCCGCACAG CAGCGCCACG 
CCCACGCCCT CGCCCACCAC CGCCGACGAG GATTTCGCCC AGCTGGACAA CAGCAAGCCC 
TTCAAGATCA AGGACATCAC GAGGAACATC CGCAAGGCAG TTGTGGCCAC AACGCTGTCG 
GAGCTGCGGA CGAAGGTGTC GCTGAAATTT GAGCGGGCTC AGCCGGCGAT ACACCTGGAT 
TGCGATGGCA CCGAGGTCGA CGATGAGGAG TACTTCAGCA CTCTGGAGCC AAATGCCGAA 
TTGATTGCCG TCTTTCCTGG CGAGCAGTGG CGCGATCCCA GTGACTACAA TGCCAATCTG 
CGTCGCACAT CGCTGGATGC ACAGCGTCTG CGGAGTCTGG TGAGCAAACT GCAGCCGAAC 
TATATGAACG ATGATGATTT GGATAAGCTG TCGAACATGG ATCCCAACTC CCTGGTGGAT 
ATCACGGGTC GGGAGCCCAA GGACAACGAA TACTCAGCCA GAAGCGATGC CGCACGGCTA 
TCAACGGAAT TGTCTTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610722.2
CDS	183..983
Translation	MTAMNADETKLAGMPQGAEEEQEPEREQKKESDGAAAAAGVQCDPDSRIVAPPPRQRTLTRTAELDADCEDIELDADDGLDDAADSITLELALSPHSSATPTPSPTTADEDFAQLDNSKPFKIKDITRNIRKAVVATTLSELRTKVSLKFERAQPAIHLDCDGTEVDDEEYFSTLEPNAELIAVFPGEQWRDPSDYNANLRRTSLDAQRLRSLVSKLQPNYMNDDDLDKLSNMDPNSLVDITGREPKDNEYSARSDAARLSTELSC

Target ORF information:

RefSeq Version	NM_136878.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster DNA fragmentation factor-related protein 3 (Drep3), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136878.3

ORF Insert Sequence:

ATGACAGCAA TGAATGCGGA TGAGACAAAG CTGGCTGGAA TGCCTCAAGG AGCTGAGGAG 
GAGCAGGAAC CGGAGAGGGA GCAGAAGAAG GAGAGCGATG GAGCAGCAGC TGCAGCAGGA 
GTACAGTGTG ATCCTGATAG CAGAATAGTG GCTCCGCCGC CCAGGCAGCG GACTCTGACA 
AGGACGGCCG AACTGGACGC GGACTGCGAG GACATCGAGT TAGATGCCGA CGATGGTTTG 
GACGATGCGG CGGATAGCAT CACCTTGGAG TTGGCCCTAT CGCCGCACAG CAGCGCCACG 
CCCACGCCCT CGCCCACCAC CGCCGACGAG GATTTCGCCC AGCTGGACAA CAGCAAGCCC 
TTCAAGATCA AGGACATCAC GAGGAACATC CGCAAGGCAG TTGTGGCCAC AACGCTGTCG 
GAGCTGCGGA CGAAGGTGTC GCTGAAATTT GAGCGGGCTC AGCCGGCGAT ACACCTGGAT 
TGCGATGGCA CCGAGGTCGA CGATGAGGAG TACTTCAGCA CTCTGGAGCC AAATGCCGAA 
TTGATTGCCG TCTTTCCTGG CGAGCAGTGG CGCGATCCCA GTGACTACAA TGCCAATCTG 
CGTCGCACAT CGCTGGATGC ACAGCGTCTG CGGAGTCTGG TGAGCAAACT GCAGCCGAAC 
TATATGAACG ATGATGATTT GGATAAGCTG TCGAACATGG ATCCCAACTC CCTGGTGGAT 
ATCACGGGTC GGGAGCCCAA GGACAACGAA TACTCAGCCA GAAGCGATGC CGCACGGCTA 
TCAACGGAAT TGTCTTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Drep3 ( NM_136879.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610723.2 Drosophila melanogaster DNA fragmentation factor-related protein 3 (Drep3), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa09678
Clone ID Related Accession (Same CDS sequence)	NM_136878.3 , NM_136879.3
Accession Version	NM_136879.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	DNA fragmentation factor-related protein 3, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136879.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGCAA TGAATGCGGA TGAGACAAAG CTGGCTGGAA TGCCTCAAGG AGCTGAGGAG 
GAGCAGGAAC CGGAGAGGGA GCAGAAGAAG GAGAGCGATG GAGCAGCAGC TGCAGCAGGA 
GTACAGTGTG ATCCTGATAG CAGAATAGTG GCTCCGCCGC CCAGGCAGCG GACTCTGACA 
AGGACGGCCG AACTGGACGC GGACTGCGAG GACATCGAGT TAGATGCCGA CGATGGTTTG 
GACGATGCGG CGGATAGCAT CACCTTGGAG TTGGCCCTAT CGCCGCACAG CAGCGCCACG 
CCCACGCCCT CGCCCACCAC CGCCGACGAG GATTTCGCCC AGCTGGACAA CAGCAAGCCC 
TTCAAGATCA AGGACATCAC GAGGAACATC CGCAAGGCAG TTGTGGCCAC AACGCTGTCG 
GAGCTGCGGA CGAAGGTGTC GCTGAAATTT GAGCGGGCTC AGCCGGCGAT ACACCTGGAT 
TGCGATGGCA CCGAGGTCGA CGATGAGGAG TACTTCAGCA CTCTGGAGCC AAATGCCGAA 
TTGATTGCCG TCTTTCCTGG CGAGCAGTGG CGCGATCCCA GTGACTACAA TGCCAATCTG 
CGTCGCACAT CGCTGGATGC ACAGCGTCTG CGGAGTCTGG TGAGCAAACT GCAGCCGAAC 
TATATGAACG ATGATGATTT GGATAAGCTG TCGAACATGG ATCCCAACTC CCTGGTGGAT 
ATCACGGGTC GGGAGCCCAA GGACAACGAA TACTCAGCCA GAAGCGATGC CGCACGGCTA 
TCAACGGAAT TGTCTTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610723.2
CDS	178..978
Translation	MTAMNADETKLAGMPQGAEEEQEPEREQKKESDGAAAAAGVQCDPDSRIVAPPPRQRTLTRTAELDADCEDIELDADDGLDDAADSITLELALSPHSSATPTPSPTTADEDFAQLDNSKPFKIKDITRNIRKAVVATTLSELRTKVSLKFERAQPAIHLDCDGTEVDDEEYFSTLEPNAELIAVFPGEQWRDPSDYNANLRRTSLDAQRLRSLVSKLQPNYMNDDDLDKLSNMDPNSLVDITGREPKDNEYSARSDAARLSTELSC

Target ORF information:

RefSeq Version	NM_136879.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster DNA fragmentation factor-related protein 3 (Drep3), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136879.3

ORF Insert Sequence:

ATGACAGCAA TGAATGCGGA TGAGACAAAG CTGGCTGGAA TGCCTCAAGG AGCTGAGGAG 
GAGCAGGAAC CGGAGAGGGA GCAGAAGAAG GAGAGCGATG GAGCAGCAGC TGCAGCAGGA 
GTACAGTGTG ATCCTGATAG CAGAATAGTG GCTCCGCCGC CCAGGCAGCG GACTCTGACA 
AGGACGGCCG AACTGGACGC GGACTGCGAG GACATCGAGT TAGATGCCGA CGATGGTTTG 
GACGATGCGG CGGATAGCAT CACCTTGGAG TTGGCCCTAT CGCCGCACAG CAGCGCCACG 
CCCACGCCCT CGCCCACCAC CGCCGACGAG GATTTCGCCC AGCTGGACAA CAGCAAGCCC 
TTCAAGATCA AGGACATCAC GAGGAACATC CGCAAGGCAG TTGTGGCCAC AACGCTGTCG 
GAGCTGCGGA CGAAGGTGTC GCTGAAATTT GAGCGGGCTC AGCCGGCGAT ACACCTGGAT 
TGCGATGGCA CCGAGGTCGA CGATGAGGAG TACTTCAGCA CTCTGGAGCC AAATGCCGAA 
TTGATTGCCG TCTTTCCTGG CGAGCAGTGG CGCGATCCCA GTGACTACAA TGCCAATCTG 
CGTCGCACAT CGCTGGATGC ACAGCGTCTG CGGAGTCTGG TGAGCAAACT GCAGCCGAAC 
TATATGAACG ATGATGATTT GGATAAGCTG TCGAACATGG ATCCCAACTC CCTGGTGGAT 
ATCACGGGTC GGGAGCCCAA GGACAACGAA TACTCAGCCA GAAGCGATGC CGCACGGCTA 
TCAACGGAAT TGTCTTGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Drep3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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