Mmp2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mmp2
Entrez Gene ID	35997
Full Name	Matrix metalloproteinase 2
Synonyms	2-MMP,CG1794,Dm2-MMP,Dmel\CG1794,MMP2,anon-WO0118547.84,dm-2MMP,dmmp2,l(2)02353,mmp2
General protein information	Preferred Names Matrix metalloproteinase 2 Names CG1794 gene product from transcript CG1794-RB CG1794-PB CG1794-PC CG1794-PD Mmp2-PB Mmp2-PC Mmp2-PD matrix metalloprotease 2 matrix metalloproteinase matrix metalloproteinase 2 matrix metalloprotinase 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 45F6-46A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273901.1	NP_001260830.1	matrix metalloproteinase 2, isoform C
NM_206066.3	NP_995788.1	matrix metalloproteinase 2, isoform B
NM_136667.3	NP_610511.3	matrix metalloproteinase 2, isoform D

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MMP17	XP_509485.2
Gallus gallus (chicken)	MMP17	XP_415092.3
Danio rerio (zebrafish)	mmp17a	XP_698601.6
Rattus norvegicus (Norway rat)	Mmp17	NP_001099395.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011870	XP_320653.4
Xenopus tropicalis (tropical clawed frog)	mmp17	NP_001096469.1
Canis lupus familiaris (dog)	MMP17	XP_852332.3
Bos taurus (cattle)	MMP17	XP_002694502.1
Mus musculus (house mouse)	Mmp17	NP_035976.3
Drosophila melanogaster (fruit fly)	Mmp2	NP_995788.1
Homo sapiens (human)	MMP17	NP_057239.4
	LOC100430708	XP_002798895.1
Caenorhabditis elegans (roundworm)	zmp-1	NP_741156.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that matrix metalloproteinases mmp1 and mmp2 mutants have distinct heart phenotypes.
Title: Matrix Metalloproteinases are required for membrane motility and lumenogenesis during Drosophila heart development.

We also show that follicular OA-Oamb signaling induces Mmp2 enzymatic activation but not Mmp2 protein expression, likely via intracellular Ca2+ as the second messenger.
Title: A Follicle Rupture Assay Reveals an Essential Role for Follicular Adrenergic Signaling in Drosophila Ovulation.

Finally, matrix metalloproteinase 2 (Mmp2), a type of protease thought to facilitate mammalian ovulation, is expressed in mature follicle and corpus luteum cells.
Title: Matrix metalloproteinase 2 is required for ovulation and corpus luteum formation in Drosophila.

As a Wnt signaling antagonist, MMP2 cleaves the glypican, reducing the ability of Dlp to interact with the Wnt ligand and promote its distribution.
Title: A matrix metalloproteinase mediates long-distance attenuation of stem cell proliferation.

Matrix metalloproteinase 2 is required for fat-body remodeling in Drosophila
Title: ?FTZ-F1 and Matrix metalloproteinase 2 are required for fat-body remodeling in Drosophila.

The following Mmp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mmp2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa09393	NM_001273901.1 Latest version!	Drosophila melanogaster matrix metalloproteinase 2, transcript variant C (Mmp2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa12339	NM_206066.3 Latest version!	Drosophila melanogaster matrix metalloproteinase 2, transcript variant B (Mmp2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa12340	NM_136667.3 Latest version!	Drosophila melanogaster matrix metalloproteinase 2, transcript variant D (Mmp2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mmp2 ( NM_001273901.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260830.1 Drosophila melanogaster matrix metalloproteinase 2, transcript variant C (Mmp2), mRNA.

Price & Availability↑

CloneID	OFa09393
Clone ID Related Accession (Same CDS sequence)	NM_001273901.1
Accession Version	NM_001273901.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	matrix metalloproteinase 2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCAACA GGTCGATGCC CGATGCCTCC AAAGTCGAGA GAATGGTGCA GACCGCCCTG 
GACGTTTGGG CGAACCACTC GAAGCTGACA TTCCGCGAGG TCTACAGCGA CCAGGCCGAC 
ATTCAGATAC TCTTTGCGCG GCGCGCGCAC GGAGATGGCT ATAAATTCGA TGGACCTGGT 
CAGGTGCTGG CCCACGCCTT CTATCCCGGC GAGGGGCGTG GCGGAGATGC CCATTTCGAT 
GCGGACGAGA CGTGGAACTT TGACGGCGAA TCCGATGATA GCCATGGAAC CAATTTTCTG 
AACGTGGCCC TGCACGAACT GGGTCATTCG CTGGGACTGG CCCACTCCGC GATCCCGGAT 
GCGGTCATGT TTCCATGGTA CCAGAATAAC GAGGTGGCGG GCAACCTGCC CGACGACGAT 
CGCTATGGCA TTCAGCAGTT GTACGGCACT AAGGAGAAGA CCTGGGGGCC ATACAAACCA 
CAAACCACAA CAACGACAAC AACCACGACG ACCATGCGCG CGATGATTTA CCGGGCAGAT 
AAACCGGCCT ACTGGCCATG GAACAATCCA AGCAACAATC CCAACAATGA TCGAAATCGT 
GCCAGGGAGC GCCAGGAGGA GGAGCGGCGG CGCCAGGAGA AGGAGCGGCG GCGCCAGGAG 
GAGGAGCGGC GGCACCAGGA GGAGGAGCGG CGGCGCCAGG TGGAGGAGCG GCAGCGCCAG 
GAAGAGGAGC GTTGGCGTCA GGAGCAGGAA CGACAAGAGG AGGAGAATCG TCGGCGCAAA 
ATAGAGCATA AAAGCCAGTG GGAGAGGAAT CCTAGCAAGG AACGGAATCG TCCCAGGGAG 
CGCCAGGAGA TGGAGAGGAG GCGTCAGGAG CAGGAGCGAC AAGAGCAGGA GCGACAGGAG 
CAGGAGGATC GTCGGCGCGA AAGAGAACGT GATCGGCAGT TGGAGTGGGA ACGTAGGAAT 
AGGAATGGAG CCCGGGAACC AGTCACTCCC ACGGCCAATA CCACCCCGAG ACCAACCAAC 
AAGCCATATC CCACGGTTCA CCGGCAGCAC CACCACCATA ACAAGCCGCG GAAACCCAAG 
CCGGACTCAT GTATGACCTA CTACGATGCC ATCTCGATTA TCCGCGGTGA ACTGTTCATC 
TTTCGGGGAC CGTACTTGTG GCGCATTGGA ACTTCTGGTC TGTATAATGG CTATCCCACG 
GAGATCAGGA GACATTGGTC CGCTCTGCCC GAAAATCTCA CCAAGGTGGA TGCTGTATAC 
GAAAACAAGC AGCGACAAAT TGTGTTCTTC ATAGGTCGCG AGTATTATGT ATTCAACTCG 
GTGATGCTAG CTCCTGGCTT CCCGAAACCA CTTGCCAGTC TGGGTCTGCC GCCCACTTTG 
ACCCACATCG ATGCCTCCTT CGTGTGGGGC CACAACAACC GCACCTACAT GACCAGCGGC 
ACACTGTACT GGCGCATCGA CGACTACACG GGTCAGGTGG AATTGGATTA CCCGCGCGAC 
ATGAGCATCT GGTCGGGAGT GGGCTACAAT ATAGATGCGG CATTCCAGTA CTTGGATGGC 
AAGACGTACT TCTTTAAGAA CCTGGGATAC TGGGAGTTCA ACGACGACCG CATGAAGGTG 
GCCCATGCCA GGGCTAAGCT CTCGGCACGA AGGTGGATGC AGTGCGCCCG CAGTGCTAAT 
GAGGTGGACG ACGAGCAGCG CTGGACGGCG TCCTTGGTAT CCGAGGGCGA GGAAACGGGG 
AGAAGCGGCT CTAGGGAGCT AAGGATCAAC CACTTCATTC TTTCGATCCT TTTGCTAGCC 
ATCGCCAACT GGCGGAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260830.1
CDS	639..2459
Translation	MVNRSMPDASKVERMVQTALDVWANHSKLTFREVYSDQADIQILFARRAHGDGYKFDGPGQVLAHAFYPGEGRGGDAHFDADETWNFDGESDDSHGTNFLNVALHELGHSLGLAHSAIPDAVMFPWYQNNEVAGNLPDDDRYGIQQLYGTKEKTWGPYKPQTTTTTTTTTTMRAMIYRADKPAYWPWNNPSNNPNNDRNRARERQEEERRRQEKERRRQEEERRHQEEERRRQVEERQRQEEERWRQEQERQEEENRRRKIEHKSQWERNPSKERNRPRERQEMERRRQEQERQEQERQEQEDRRRERERDRQLEWERRNRNGAREPVTPTANTTPRPTNKPYPTVHRQHHHHNKPRKPKPDSCMTYYDAISIIRGELFIFRGPYLWRIGTSGLYNGYPTEIRRHWSALPENLTKVDAVYENKQRQIVFFIGREYYVFNSVMLAPGFPKPLASLGLPPTLTHIDASFVWGHNNRTYMTSGTLYWRIDDYTGQVELDYPRDMSIWSGVGYNIDAAFQYLDGKTYFFKNLGYWEFNDDRMKVAHARAKLSARRWMQCARSANEVDDEQRWTASLVSEGEETGRSGSRELRINHFILSILLLAIANWRS

Target ORF information:

RefSeq Version	NM_001273901.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster matrix metalloproteinase 2, transcript variant C (Mmp2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273901.1

ORF Insert Sequence:

ATGGTCAACA GGTCGATGCC CGATGCCTCC AAAGTCGAGA GAATGGTGCA GACCGCCCTG 
GACGTTTGGG CGAACCACTC GAAGCTGACA TTCCGCGAGG TCTACAGCGA CCAGGCCGAC 
ATTCAGATAC TCTTTGCGCG GCGCGCGCAC GGAGATGGCT ATAAATTCGA TGGACCTGGT 
CAGGTGCTGG CCCACGCCTT CTATCCCGGC GAGGGGCGTG GCGGAGATGC CCATTTCGAT 
GCGGACGAGA CGTGGAACTT TGACGGCGAA TCCGATGATA GCCATGGAAC CAATTTTCTG 
AACGTGGCCC TGCACGAACT GGGTCATTCG CTGGGACTGG CCCACTCCGC GATCCCGGAT 
GCGGTCATGT TTCCATGGTA CCAGAATAAC GAGGTGGCGG GCAACCTGCC CGACGACGAT 
CGCTATGGCA TTCAGCAGTT GTACGGCACT AAGGAGAAGA CCTGGGGGCC ATACAAACCA 
CAAACCACAA CAACGACAAC AACCACGACG ACCATGCGCG CGATGATTTA CCGGGCAGAT 
AAACCGGCCT ACTGGCCATG GAACAATCCA AGCAACAATC CCAACAATGA TCGAAATCGT 
GCCAGGGAGC GCCAGGAGGA GGAGCGGCGG CGCCAGGAGA AGGAGCGGCG GCGCCAGGAG 
GAGGAGCGGC GGCACCAGGA GGAGGAGCGG CGGCGCCAGG TGGAGGAGCG GCAGCGCCAG 
GAAGAGGAGC GTTGGCGTCA GGAGCAGGAA CGACAAGAGG AGGAGAATCG TCGGCGCAAA 
ATAGAGCATA AAAGCCAGTG GGAGAGGAAT CCTAGCAAGG AACGGAATCG TCCCAGGGAG 
CGCCAGGAGA TGGAGAGGAG GCGTCAGGAG CAGGAGCGAC AAGAGCAGGA GCGACAGGAG 
CAGGAGGATC GTCGGCGCGA AAGAGAACGT GATCGGCAGT TGGAGTGGGA ACGTAGGAAT 
AGGAATGGAG CCCGGGAACC AGTCACTCCC ACGGCCAATA CCACCCCGAG ACCAACCAAC 
AAGCCATATC CCACGGTTCA CCGGCAGCAC CACCACCATA ACAAGCCGCG GAAACCCAAG 
CCGGACTCAT GTATGACCTA CTACGATGCC ATCTCGATTA TCCGCGGTGA ACTGTTCATC 
TTTCGGGGAC CGTACTTGTG GCGCATTGGA ACTTCTGGTC TGTATAATGG CTATCCCACG 
GAGATCAGGA GACATTGGTC CGCTCTGCCC GAAAATCTCA CCAAGGTGGA TGCTGTATAC 
GAAAACAAGC AGCGACAAAT TGTGTTCTTC ATAGGTCGCG AGTATTATGT ATTCAACTCG 
GTGATGCTAG CTCCTGGCTT CCCGAAACCA CTTGCCAGTC TGGGTCTGCC GCCCACTTTG 
ACCCACATCG ATGCCTCCTT CGTGTGGGGC CACAACAACC GCACCTACAT GACCAGCGGC 
ACACTGTACT GGCGCATCGA CGACTACACG GGTCAGGTGG AATTGGATTA CCCGCGCGAC 
ATGAGCATCT GGTCGGGAGT GGGCTACAAT ATAGATGCGG CATTCCAGTA CTTGGATGGC 
AAGACGTACT TCTTTAAGAA CCTGGGATAC TGGGAGTTCA ACGACGACCG CATGAAGGTG 
GCCCATGCCA GGGCTAAGCT CTCGGCACGA AGGTGGATGC AGTGCGCCCG CAGTGCTAAT 
GAGGTGGACG ACGAGCAGCG CTGGACGGCG TCCTTGGTAT CCGAGGGCGA GGAAACGGGG 
AGAAGCGGCT CTAGGGAGCT AAGGATCAAC CACTTCATTC TTTCGATCCT TTTGCTAGCC 
ATCGCCAACT GGCGGAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mmp2 ( NM_206066.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995788.1 Drosophila melanogaster matrix metalloproteinase 2, transcript variant B (Mmp2), mRNA.

Price & Availability↑

CloneID	OFa12339
Clone ID Related Accession (Same CDS sequence)	NM_206066.3
Accession Version	NM_206066.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	matrix metalloproteinase 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_206066.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTTCCA AATATGTTCT CGCCACCCTG CTCGCACTCT TTGCGCAATC CATGTGCATC 
CAGGAGCTGT CCCTCCCACC CGAAGGATCT CACTCCACCG CTGCGACCAG GTCCAAGAAG 
GCAAAAACCG CTATCTCCGA GGATATAATG TACAATTACC TCATGCAGTT TGATTATCTG 
CCCAAGTCGG ACCTGGAAAC GGGTGCTCTG CGCACCGAGG ACCAGCTGAA AGAAGCAATC 
CGGAGTCTCC AGTCTTTTGG CAACATTACA GTTACGGGCG AAATCGACTC GGCAACGGCC 
CGGCTAATAC AGAAACCTCG GTGCGGCGTG GGCGACAGAA GGTCCGCGGA CAGCTTCTCG 
CCAGATAACC TGTATCACGA AATCGGCTCC AATGTGCGGG TGCGGCGCTT CGCCCTGCAG 
GGACCCAAGT GGTCCAGAAC GGATCTTACG TGGAGCATGG TCAACAGGTC GATGCCCGAT 
GCCTCCAAAG TCGAGAGAAT GGTGCAGACC GCCCTGGACG TTTGGGCGAA CCACTCGAAG 
CTGACATTCC GCGAGGTCTA CAGCGACCAG GCCGACATTC AGATACTCTT TGCGCGGCGC 
GCGCACGGAG ATGGCTATAA ATTCGATGGA CCTGGTCAGG TGCTGGCCCA CGCCTTCTAT 
CCCGGCGAGG GGCGTGGCGG AGATGCCCAT TTCGATGCGG ACGAGACGTG GAACTTTGAC 
GGCGAATCCG ATGATAGCCA TGGAACCAAT TTTCTGAACG TGGCCCTGCA CGAACTGGGT 
CATTCGCTGG GACTGGCCCA CTCCGCGATC CCGGATGCGG TCATGTTTCC ATGGTACCAG 
AATAACGAGG TGGCGGGCAA CCTGCCCGAC GACGATCGCT ATGGCATTCA GCAGTTGTAC 
GGCACTAAGG AGAAGACCTG GGGGCCATAC AAACCACAAA CCACAACAAC GACAACAACC 
ACGACGACCA TGCGCGCGAT GATTTACCGG GCAGATAAAC CGGCCTACTG GCCATGGAAC 
AATCCAAGCA ACAATCCCAA CAATGATCGA AATCGTGCCA GGGAGCGCCA GGAGGAGGAG 
CGGCGGCGCC AGGAGAAGGA GCGGCGGCGC CAGGAGGAGG AGCGGCGGCA CCAGGAGGAG 
GAGCGGCGGC GCCAGGTGGA GGAGCGGCAG CGCCAGGAAG AGGAGCGTTG GCGTCAGGAG 
CAGGAACGAC AAGAGGAGGA GAATCGTCGG CGCAAAATAG AGCATAAAAG CCAGTGGGAG 
AGGAATCCTA GCAAGGAACG GAATCGTCCC AGGGAGCGCC AGGAGATGGA GAGGAGGCGT 
CAGGAGCAGG AGCGACAAGA GCAGGAGCGA CAGGAGCAGG AGGATCGTCG GCGCGAAAGA 
GAACGTGATC GGCAGTTGGA GTGGGAACGT AGGAATAGGA ATGGAGCCCG GGAACCAGTC 
ACTCCCACGG CCAATACCAC CCCGAGACCA ACCAACAAGC CATATCCCAC GGTTCACCGG 
CAGCACCACC ACCATAACAA GCCGCGGAAA CCCAAGCCGG ACTCATGTAT GACCTACTAC 
GATGCCATCT CGATTATCCG CGGTGAACTG TTCATCTTTC GGGGACCGTA CTTGTGGCGC 
ATTGGAACTT CTGGTCTGTA TAATGGCTAT CCCACGGAGA TCAGGAGACA TTGGTCCGCT 
CTGCCCGAAA ATCTCACCAA GGTGGATGCT GTATACGAAA ACAAGCAGCG ACAAATTGTG 
TTCTTCATAG GTCGCGAGTA TTATGTATTC AACTCGGTGA TGCTAGCTCC TGGCTTCCCG 
AAACCACTTG CCAGTCTGGG TCTGCCGCCC ACTTTGACCC ACATCGATGC CTCCTTCGTG 
TGGGGCCACA ACAACCGCAC CTACATGACC AGCGGCACAC TGTACTGGCG CATCGACGAC 
TACACGGGTC AGGTGGAATT GGATTACCCG CGCGACATGA GCATCTGGTC GGGAGTGGGC 
TACAATATAG ATGCGGCATT CCAGTACTTG GATGGCAAGA CGTACTTCTT TAAGAACCTG 
GGATACTGGG AGTTCAACGA CGACCGCATG AAGGTGGCCC ATGCCAGGGC TAAGCTCTCG 
GCACGAAGGT GGATGCAGTG CGCCCGCAGT GCTAATGAGG TGGACGACGA GCAGCGCTGG 
ACGGCGTCCT TGGTATCCGA GGGCGAGGAA ACGGGGAGAA GCGGCTCTAG GGAGCTAAGG 
ATCAACCACT TCATTCTTTC GATCCTTTTG CTAGCCATCG CCAACTGGCG GAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995788.1
CDS	927..3203
Translation	MFSKYVLATLLALFAQSMCIQELSLPPEGSHSTAATRSKKAKTAISEDIMYNYLMQFDYLPKSDLETGALRTEDQLKEAIRSLQSFGNITVTGEIDSATARLIQKPRCGVGDRRSADSFSPDNLYHEIGSNVRVRRFALQGPKWSRTDLTWSMVNRSMPDASKVERMVQTALDVWANHSKLTFREVYSDQADIQILFARRAHGDGYKFDGPGQVLAHAFYPGEGRGGDAHFDADETWNFDGESDDSHGTNFLNVALHELGHSLGLAHSAIPDAVMFPWYQNNEVAGNLPDDDRYGIQQLYGTKEKTWGPYKPQTTTTTTTTTTMRAMIYRADKPAYWPWNNPSNNPNNDRNRARERQEEERRRQEKERRRQEEERRHQEEERRRQVEERQRQEEERWRQEQERQEEENRRRKIEHKSQWERNPSKERNRPRERQEMERRRQEQERQEQERQEQEDRRRERERDRQLEWERRNRNGAREPVTPTANTTPRPTNKPYPTVHRQHHHHNKPRKPKPDSCMTYYDAISIIRGELFIFRGPYLWRIGTSGLYNGYPTEIRRHWSALPENLTKVDAVYENKQRQIVFFIGREYYVFNSVMLAPGFPKPLASLGLPPTLTHIDASFVWGHNNRTYMTSGTLYWRIDDYTGQVELDYPRDMSIWSGVGYNIDAAFQYLDGKTYFFKNLGYWEFNDDRMKVAHARAKLSARRWMQCARSANEVDDEQRWTASLVSEGEETGRSGSRELRINHFILSILLLAIANWRS

Target ORF information:

RefSeq Version	NM_206066.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster matrix metalloproteinase 2, transcript variant B (Mmp2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206066.3

ORF Insert Sequence:

ATGTTTTCCA AATATGTTCT CGCCACCCTG CTCGCACTCT TTGCGCAATC CATGTGCATC 
CAGGAGCTGT CCCTCCCACC CGAAGGATCT CACTCCACCG CTGCGACCAG GTCCAAGAAG 
GCAAAAACCG CTATCTCCGA GGATATAATG TACAATTACC TCATGCAGTT TGATTATCTG 
CCCAAGTCGG ACCTGGAAAC GGGTGCTCTG CGCACCGAGG ACCAGCTGAA AGAAGCAATC 
CGGAGTCTCC AGTCTTTTGG CAACATTACA GTTACGGGCG AAATCGACTC GGCAACGGCC 
CGGCTAATAC AGAAACCTCG GTGCGGCGTG GGCGACAGAA GGTCCGCGGA CAGCTTCTCG 
CCAGATAACC TGTATCACGA AATCGGCTCC AATGTGCGGG TGCGGCGCTT CGCCCTGCAG 
GGACCCAAGT GGTCCAGAAC GGATCTTACG TGGAGCATGG TCAACAGGTC GATGCCCGAT 
GCCTCCAAAG TCGAGAGAAT GGTGCAGACC GCCCTGGACG TTTGGGCGAA CCACTCGAAG 
CTGACATTCC GCGAGGTCTA CAGCGACCAG GCCGACATTC AGATACTCTT TGCGCGGCGC 
GCGCACGGAG ATGGCTATAA ATTCGATGGA CCTGGTCAGG TGCTGGCCCA CGCCTTCTAT 
CCCGGCGAGG GGCGTGGCGG AGATGCCCAT TTCGATGCGG ACGAGACGTG GAACTTTGAC 
GGCGAATCCG ATGATAGCCA TGGAACCAAT TTTCTGAACG TGGCCCTGCA CGAACTGGGT 
CATTCGCTGG GACTGGCCCA CTCCGCGATC CCGGATGCGG TCATGTTTCC ATGGTACCAG 
AATAACGAGG TGGCGGGCAA CCTGCCCGAC GACGATCGCT ATGGCATTCA GCAGTTGTAC 
GGCACTAAGG AGAAGACCTG GGGGCCATAC AAACCACAAA CCACAACAAC GACAACAACC 
ACGACGACCA TGCGCGCGAT GATTTACCGG GCAGATAAAC CGGCCTACTG GCCATGGAAC 
AATCCAAGCA ACAATCCCAA CAATGATCGA AATCGTGCCA GGGAGCGCCA GGAGGAGGAG 
CGGCGGCGCC AGGAGAAGGA GCGGCGGCGC CAGGAGGAGG AGCGGCGGCA CCAGGAGGAG 
GAGCGGCGGC GCCAGGTGGA GGAGCGGCAG CGCCAGGAAG AGGAGCGTTG GCGTCAGGAG 
CAGGAACGAC AAGAGGAGGA GAATCGTCGG CGCAAAATAG AGCATAAAAG CCAGTGGGAG 
AGGAATCCTA GCAAGGAACG GAATCGTCCC AGGGAGCGCC AGGAGATGGA GAGGAGGCGT 
CAGGAGCAGG AGCGACAAGA GCAGGAGCGA CAGGAGCAGG AGGATCGTCG GCGCGAAAGA 
GAACGTGATC GGCAGTTGGA GTGGGAACGT AGGAATAGGA ATGGAGCCCG GGAACCAGTC 
ACTCCCACGG CCAATACCAC CCCGAGACCA ACCAACAAGC CATATCCCAC GGTTCACCGG 
CAGCACCACC ACCATAACAA GCCGCGGAAA CCCAAGCCGG ACTCATGTAT GACCTACTAC 
GATGCCATCT CGATTATCCG CGGTGAACTG TTCATCTTTC GGGGACCGTA CTTGTGGCGC 
ATTGGAACTT CTGGTCTGTA TAATGGCTAT CCCACGGAGA TCAGGAGACA TTGGTCCGCT 
CTGCCCGAAA ATCTCACCAA GGTGGATGCT GTATACGAAA ACAAGCAGCG ACAAATTGTG 
TTCTTCATAG GTCGCGAGTA TTATGTATTC AACTCGGTGA TGCTAGCTCC TGGCTTCCCG 
AAACCACTTG CCAGTCTGGG TCTGCCGCCC ACTTTGACCC ACATCGATGC CTCCTTCGTG 
TGGGGCCACA ACAACCGCAC CTACATGACC AGCGGCACAC TGTACTGGCG CATCGACGAC 
TACACGGGTC AGGTGGAATT GGATTACCCG CGCGACATGA GCATCTGGTC GGGAGTGGGC 
TACAATATAG ATGCGGCATT CCAGTACTTG GATGGCAAGA CGTACTTCTT TAAGAACCTG 
GGATACTGGG AGTTCAACGA CGACCGCATG AAGGTGGCCC ATGCCAGGGC TAAGCTCTCG 
GCACGAAGGT GGATGCAGTG CGCCCGCAGT GCTAATGAGG TGGACGACGA GCAGCGCTGG 
ACGGCGTCCT TGGTATCCGA GGGCGAGGAA ACGGGGAGAA GCGGCTCTAG GGAGCTAAGG 
ATCAACCACT TCATTCTTTC GATCCTTTTG CTAGCCATCG CCAACTGGCG GAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mmp2 ( NM_136667.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610511.3 Drosophila melanogaster matrix metalloproteinase 2, transcript variant D (Mmp2), mRNA.

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CloneID	OFa12340
Clone ID Related Accession (Same CDS sequence)	NM_136667.3
Accession Version	NM_136667.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	matrix metalloproteinase 2, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_136667.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGTCGT TATGGCCAAG GCGACAGTTT TCCGCTGCAG CAGTCTTGCT CCATTTCGGC 
TGCACTTGGT CTTTAGTCCT TGCCACTCGA CTCGCTGTAA TTTCTTGGCG TTTATTTTTG 
TGCTTTCTGC GCATGGTCAA CAGGTCGATG CCCGATGCCT CCAAAGTCGA GAGAATGGTG 
CAGACCGCCC TGGACGTTTG GGCGAACCAC TCGAAGCTGA CATTCCGCGA GGTCTACAGC 
GACCAGGCCG ACATTCAGAT ACTCTTTGCG CGGCGCGCGC ACGGAGATGG CTATAAATTC 
GATGGACCTG GTCAGGTGCT GGCCCACGCC TTCTATCCCG GCGAGGGGCG TGGCGGAGAT 
GCCCATTTCG ATGCGGACGA GACGTGGAAC TTTGACGGCG AATCCGATGA TAGCCATGGA 
ACCAATTTTC TGAACGTGGC CCTGCACGAA CTGGGTCATT CGCTGGGACT GGCCCACTCC 
GCGATCCCGG ATGCGGTCAT GTTTCCATGG TACCAGAATA ACGAGGTGGC GGGCAACCTG 
CCCGACGACG ATCGCTATGG CATTCAGCAG TTGTACGGCA CTAAGGAGAA GACCTGGGGG 
CCATACAAAC CACAAACCAC AACAACGACA ACAACCACGA CGACCATGCG CGCGATGATT 
TACCGGGCAG ATAAACCGGC CTACTGGCCA TGGAACAATC CAAGCAACAA TCCCAACAAT 
GATCGAAATC GTGCCAGGGA GCGCCAGGAG GAGGAGCGGC GGCGCCAGGA GAAGGAGCGG 
CGGCGCCAGG AGGAGGAGCG GCGGCACCAG GAGGAGGAGC GGCGGCGCCA GGTGGAGGAG 
CGGCAGCGCC AGGAAGAGGA GCGTTGGCGT CAGGAGCAGG AACGACAAGA GGAGGAGAAT 
CGTCGGCGCA AAATAGAGCA TAAAAGCCAG TGGGAGAGGA ATCCTAGCAA GGAACGGAAT 
CGTCCCAGGG AGCGCCAGGA GATGGAGAGG AGGCGTCAGG AGCAGGAGCG ACAAGAGCAG 
GAGCGACAGG AGCAGGAGGA TCGTCGGCGC GAAAGAGAAC GTGATCGGCA GTTGGAGTGG 
GAACGTAGGA ATAGGAATGG AGCCCGGGAA CCAGTCACTC CCACGGCCAA TACCACCCCG 
AGACCAACCA ACAAGCCATA TCCCACGGTT CACCGGCAGC ACCACCACCA TAACAAGCCG 
CGGAAACCCA AGCCGGACTC ATGTATGACC TACTACGATG CCATCTCGAT TATCCGCGGT 
GAACTGTTCA TCTTTCGGGG ACCGTACTTG TGGCGCATTG GAACTTCTGG TCTGTATAAT 
GGCTATCCCA CGGAGATCAG GAGACATTGG TCCGCTCTGC CCGAAAATCT CACCAAGGTG 
GATGCTGTAT ACGAAAACAA GCAGCGACAA ATTGTGTTCT TCATAGGTCG CGAGTATTAT 
GTATTCAACT CGGTGATGCT AGCTCCTGGC TTCCCGAAAC CACTTGCCAG TCTGGGTCTG 
CCGCCCACTT TGACCCACAT CGATGCCTCC TTCGTGTGGG GCCACAACAA CCGCACCTAC 
ATGACCAGCG GCACACTGTA CTGGCGCATC GACGACTACA CGGGTCAGGT GGAATTGGAT 
TACCCGCGCG ACATGAGCAT CTGGTCGGGA GTGGGCTACA ATATAGATGC GGCATTCCAG 
TACTTGGATG GCAAGACGTA CTTCTTTAAG AACCTGGGAT ACTGGGAGTT CAACGACGAC 
CGCATGAAGG TGGCCCATGC CAGGGCTAAG CTCTCGGCAC GAAGGTGGAT GCAGTGCGCC 
CGCAGTGCTA ATGAGGTGGA CGACGAGCAG CGCTGGACGG CGTCCTTGGT ATCCGAGGGC 
GAGGAAACGG GGAGAAGCGG CTCTAGGGAG CTAAGGATCA ACCACTTCAT TCTTTCGATC 
CTTTTGCTAG CCATCGCCAA CTGGCGGAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610511.3
CDS	779..2731
Translation	MLSLWPRRQFSAAAVLLHFGCTWSLVLATRLAVISWRLFLCFLRMVNRSMPDASKVERMVQTALDVWANHSKLTFREVYSDQADIQILFARRAHGDGYKFDGPGQVLAHAFYPGEGRGGDAHFDADETWNFDGESDDSHGTNFLNVALHELGHSLGLAHSAIPDAVMFPWYQNNEVAGNLPDDDRYGIQQLYGTKEKTWGPYKPQTTTTTTTTTTMRAMIYRADKPAYWPWNNPSNNPNNDRNRARERQEEERRRQEKERRRQEEERRHQEEERRRQVEERQRQEEERWRQEQERQEEENRRRKIEHKSQWERNPSKERNRPRERQEMERRRQEQERQEQERQEQEDRRRERERDRQLEWERRNRNGAREPVTPTANTTPRPTNKPYPTVHRQHHHHNKPRKPKPDSCMTYYDAISIIRGELFIFRGPYLWRIGTSGLYNGYPTEIRRHWSALPENLTKVDAVYENKQRQIVFFIGREYYVFNSVMLAPGFPKPLASLGLPPTLTHIDASFVWGHNNRTYMTSGTLYWRIDDYTGQVELDYPRDMSIWSGVGYNIDAAFQYLDGKTYFFKNLGYWEFNDDRMKVAHARAKLSARRWMQCARSANEVDDEQRWTASLVSEGEETGRSGSRELRINHFILSILLLAIANWRS

Target ORF information:

RefSeq Version	NM_136667.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster matrix metalloproteinase 2, transcript variant D (Mmp2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136667.3

ORF Insert Sequence:

ATGCTGTCGT TATGGCCAAG GCGACAGTTT TCCGCTGCAG CAGTCTTGCT CCATTTCGGC 
TGCACTTGGT CTTTAGTCCT TGCCACTCGA CTCGCTGTAA TTTCTTGGCG TTTATTTTTG 
TGCTTTCTGC GCATGGTCAA CAGGTCGATG CCCGATGCCT CCAAAGTCGA GAGAATGGTG 
CAGACCGCCC TGGACGTTTG GGCGAACCAC TCGAAGCTGA CATTCCGCGA GGTCTACAGC 
GACCAGGCCG ACATTCAGAT ACTCTTTGCG CGGCGCGCGC ACGGAGATGG CTATAAATTC 
GATGGACCTG GTCAGGTGCT GGCCCACGCC TTCTATCCCG GCGAGGGGCG TGGCGGAGAT 
GCCCATTTCG ATGCGGACGA GACGTGGAAC TTTGACGGCG AATCCGATGA TAGCCATGGA 
ACCAATTTTC TGAACGTGGC CCTGCACGAA CTGGGTCATT CGCTGGGACT GGCCCACTCC 
GCGATCCCGG ATGCGGTCAT GTTTCCATGG TACCAGAATA ACGAGGTGGC GGGCAACCTG 
CCCGACGACG ATCGCTATGG CATTCAGCAG TTGTACGGCA CTAAGGAGAA GACCTGGGGG 
CCATACAAAC CACAAACCAC AACAACGACA ACAACCACGA CGACCATGCG CGCGATGATT 
TACCGGGCAG ATAAACCGGC CTACTGGCCA TGGAACAATC CAAGCAACAA TCCCAACAAT 
GATCGAAATC GTGCCAGGGA GCGCCAGGAG GAGGAGCGGC GGCGCCAGGA GAAGGAGCGG 
CGGCGCCAGG AGGAGGAGCG GCGGCACCAG GAGGAGGAGC GGCGGCGCCA GGTGGAGGAG 
CGGCAGCGCC AGGAAGAGGA GCGTTGGCGT CAGGAGCAGG AACGACAAGA GGAGGAGAAT 
CGTCGGCGCA AAATAGAGCA TAAAAGCCAG TGGGAGAGGA ATCCTAGCAA GGAACGGAAT 
CGTCCCAGGG AGCGCCAGGA GATGGAGAGG AGGCGTCAGG AGCAGGAGCG ACAAGAGCAG 
GAGCGACAGG AGCAGGAGGA TCGTCGGCGC GAAAGAGAAC GTGATCGGCA GTTGGAGTGG 
GAACGTAGGA ATAGGAATGG AGCCCGGGAA CCAGTCACTC CCACGGCCAA TACCACCCCG 
AGACCAACCA ACAAGCCATA TCCCACGGTT CACCGGCAGC ACCACCACCA TAACAAGCCG 
CGGAAACCCA AGCCGGACTC ATGTATGACC TACTACGATG CCATCTCGAT TATCCGCGGT 
GAACTGTTCA TCTTTCGGGG ACCGTACTTG TGGCGCATTG GAACTTCTGG TCTGTATAAT 
GGCTATCCCA CGGAGATCAG GAGACATTGG TCCGCTCTGC CCGAAAATCT CACCAAGGTG 
GATGCTGTAT ACGAAAACAA GCAGCGACAA ATTGTGTTCT TCATAGGTCG CGAGTATTAT 
GTATTCAACT CGGTGATGCT AGCTCCTGGC TTCCCGAAAC CACTTGCCAG TCTGGGTCTG 
CCGCCCACTT TGACCCACAT CGATGCCTCC TTCGTGTGGG GCCACAACAA CCGCACCTAC 
ATGACCAGCG GCACACTGTA CTGGCGCATC GACGACTACA CGGGTCAGGT GGAATTGGAT 
TACCCGCGCG ACATGAGCAT CTGGTCGGGA GTGGGCTACA ATATAGATGC GGCATTCCAG 
TACTTGGATG GCAAGACGTA CTTCTTTAAG AACCTGGGAT ACTGGGAGTT CAACGACGAC 
CGCATGAAGG TGGCCCATGC CAGGGCTAAG CTCTCGGCAC GAAGGTGGAT GCAGTGCGCC 
CGCAGTGCTA ATGAGGTGGA CGACGAGCAG CGCTGGACGG CGTCCTTGGT ATCCGAGGGC 
GAGGAAACGG GGAGAAGCGG CTCTAGGGAG CTAAGGATCA ACCACTTCAT TCTTTCGATC 
CTTTTGCTAG CCATCGCCAA CTGGCGGAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Mmp2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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