CG8027 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG8027
Entrez Gene ID	35943
Full Name	CG8027 gene product from transcript CG8027-RA
Synonyms	Cg8027,Dmel\CG8027
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 45B3-45B4

mRNA and Protein(s)

mRNA	Protein	Name
NM_136625.3	NP_610469.3	uncharacterized protein

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012336	XP_320212.4
Drosophila melanogaster (fruit fly)	CG8027	NP_610469.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG8027 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG8027 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09361	NM_136625.3 Latest version!	Drosophila melanogaster uncharacterized protein (CG8027), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG8027 ( NM_136625.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610469.3 Drosophila melanogaster uncharacterized protein (CG8027), mRNA.

Price & Availability↑

CloneID	OFa09361
Clone ID Related Accession (Same CDS sequence)	NM_136625.3
Accession Version	NM_136625.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_136625.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGATTAC GCCGCCGTTG GCCGCGAAGG ATGCGGCGTG CCATGGAACA ACTGCGTCTC 
CAGTCCCGCC GGAAGCTGCT GGCGCTCACG ATGACTGGAG GACTCTGCCT GGCTATCTGG 
GTAGTTGCCA ACTATCTGTC CGCATCGGAG GAGGGCCAAA CCGGCGGCTT CAGCAGCGCC 
TGCACTGCCA TCGATGCGGT TTACACGTGG GTCAATGGCT CGGATCCCAA CTTTATTGAG 
GACATCCGGC GATTCGATGA CAAGTACGAT CCCTCGCGGT TCGATGACAA GAATGAGCTG 
CGGTACTCTC TGAGGTCCCT GGAAAAACAC GCCGCATGGA TCAGGCATGT GTACATAGTA 
ACCAATGGCC AGATTCCAAG TTGGCTGGAT CTCAGCTACG AAAGGGTCAC GGTGGTGCCC 
CACGAAGTCC TGGCTCCCGA TCCCGACCAG CTGCCCACCT TCTCCAGCTC GGCCATCGAG 
ACATTTCTGC ACCGCATACC AAAGCTGTCC AAGAGGTTCC TCTACCTCAA CGACGACATA 
TTCCTGGGAG CTCCGCTGTA TCCGGAGGAC TTGTACACTG AAGCGGAGGG AGTTCGCGTG 
TACCAGGCAT GGATGGTGCC CGGCTGCGCC TTGGATTGCC CCTGGACGTA CATAGGTGAT 
GGAGCTTGCG ATCGGCACTG CAACATTGAT GCGTGCCAAT TTGATGGAGG CGACTGCAGT 
GAAACTGGGC CAGCGAGCGA TGCCCACGTC ATTCCACCAA GCAAAGAAGT GCTCGAGGTG 
CAGCCTGCCG CTGTTCCACA ATCAAGAGTC CACCGATTTC CTCAGATGGG TCTCCAAAAG 
CTGTTCAGGC GCAGCTCTGC CAATTTTAAG GATGTTATGC GGCACCGCAA TGTGTCCACA 
CTCAAGGAAC TACGTCGCAT TGTGGAGCGT TTTAACAAGG CCAAACTCAT GTCGCTGAAC 
CCCGAACTGG AGACCTCCAG CTCCGAGCCA CAGACAACTC AGCGCCACGG GCTGCGCAAG 
GAGGATTTTA AGTCTTCCAC CGATATTTAC TCTCACTCGC TGATTGCCAC CAATATGTTG 
CTGAATAGAG CCTATGGCTT TAAGGCACGC CATGTCCTGG CGCACGTGGG CTTCCTAATT 
GACAAGGATA TTGTGGAGGC CATGCAGCGA CGTTTTCACC AGCAAATTCT GGACACTGCC 
CATCAGCGCT TCCGAGCCCC AACAGATTTG CAGTACGCAT TCGCTTACTA CTCCTTCCTA 
ATGAGCGAAA CCAAAGTAAT GAGTGTAGAG GAAATCTTCG ATGAGTTCGA CACGGACGGT 
TCGGCCACCT GGTCGGATCG AGAGGTGCGA ACCTTTCTCA CTCGCATCTA TCAGCCGCCA 
CTTGACTGGT CTGCCATGCG CTACTTCGAG GAGGTGGTTC AAAACTGCAC AAGAAATCTG 
GGCATGCATT TAAAAGTGGA CACAGTCGAA CACTCCACGC TGGTCTACGA GCGATACGAG 
GACTCTAATT TGCCTACAAT AACCAGGGAT TTGGTTGTCC GATGTCCACT TCTGGCCGAA 
GCTTTGGCGG CAAACTTTGC AGTCCGACCC AAATATAACT TCCATGTCAG CCCAAAAAGA 
ACTTCGCACA GCAATTTTAT GATGCTCACA TCGAATCTCA CGGAGGTGGT GGAGTCACTG 
GACCGATTGC GTCGGAATCC GCGCAAGTTT AACTGCATCA ACGACAATCT GGATGCCAAT 
AGAGGCGAAG ACAACGAGAT GGTGCGCCAT CTGCTCGAGG ACTTTTACCT CTCCTTCTTT 
CCGAGACGCA GCAAGTTCGA GCTGCCGCCG CAATATCGTA ATCGGTTCGA GTCCTGGCGG 
GACTTTCAGC GCTGGAAGAG AAGAAAGCGG GCGGTTCTCG TGATTGGATA TGGAGTGAGT 
CTGCTGCTTG TGGTCTGTCT GCTGCGATTC ATGTGCCACC ACAAGGCGAA GCTGGTGAGG 
CGCTGTGTGC AGCGCTTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610469.3
CDS	101..2101
Translation	MRLRRRWPRRMRRAMEQLRLQSRRKLLALTMTGGLCLAIWVVANYLSASEEGQTGGFSSACTAIDAVYTWVNGSDPNFIEDIRRFDDKYDPSRFDDKNELRYSLRSLEKHAAWIRHVYIVTNGQIPSWLDLSYERVTVVPHEVLAPDPDQLPTFSSSAIETFLHRIPKLSKRFLYLNDDIFLGAPLYPEDLYTEAEGVRVYQAWMVPGCALDCPWTYIGDGACDRHCNIDACQFDGGDCSETGPASDAHVIPPSKEVLEVQPAAVPQSRVHRFPQMGLQKLFRRSSANFKDVMRHRNVSTLKELRRIVERFNKAKLMSLNPELETSSSEPQTTQRHGLRKEDFKSSTDIYSHSLIATNMLLNRAYGFKARHVLAHVGFLIDKDIVEAMQRRFHQQILDTAHQRFRAPTDLQYAFAYYSFLMSETKVMSVEEIFDEFDTDGSATWSDREVRTFLTRIYQPPLDWSAMRYFEEVVQNCTRNLGMHLKVDTVEHSTLVYERYEDSNLPTITRDLVVRCPLLAEALAANFAVRPKYNFHVSPKRTSHSNFMMLTSNLTEVVESLDRLRRNPRKFNCINDNLDANRGEDNEMVRHLLEDFYLSFFPRRSKFELPPQYRNRFESWRDFQRWKRRKRAVLVIGYGVSLLLVVCLLRFMCHHKAKLVRRCVQRL

Target ORF information:

RefSeq Version	NM_136625.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein (CG8027), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136625.3

ORF Insert Sequence:

ATGCGATTAC GCCGCCGTTG GCCGCGAAGG ATGCGGCGTG CCATGGAACA ACTGCGTCTC 
CAGTCCCGCC GGAAGCTGCT GGCGCTCACG ATGACTGGAG GACTCTGCCT GGCTATCTGG 
GTAGTTGCCA ACTATCTGTC CGCATCGGAG GAGGGCCAAA CCGGCGGCTT CAGCAGCGCC 
TGCACTGCCA TCGATGCGGT TTACACGTGG GTCAATGGCT CGGATCCCAA CTTTATTGAG 
GACATCCGGC GATTCGATGA CAAGTACGAT CCCTCGCGGT TCGATGACAA GAATGAGCTG 
CGGTACTCTC TGAGGTCCCT GGAAAAACAC GCCGCATGGA TCAGGCATGT GTACATAGTA 
ACCAATGGCC AGATTCCAAG TTGGCTGGAT CTCAGCTACG AAAGGGTCAC GGTGGTGCCC 
CACGAAGTCC TGGCTCCCGA TCCCGACCAG CTGCCCACCT TCTCCAGCTC GGCCATCGAG 
ACATTTCTGC ACCGCATACC AAAGCTGTCC AAGAGGTTCC TCTACCTCAA CGACGACATA 
TTCCTGGGAG CTCCGCTGTA TCCGGAGGAC TTGTACACTG AAGCGGAGGG AGTTCGCGTG 
TACCAGGCAT GGATGGTGCC CGGCTGCGCC TTGGATTGCC CCTGGACGTA CATAGGTGAT 
GGAGCTTGCG ATCGGCACTG CAACATTGAT GCGTGCCAAT TTGATGGAGG CGACTGCAGT 
GAAACTGGGC CAGCGAGCGA TGCCCACGTC ATTCCACCAA GCAAAGAAGT GCTCGAGGTG 
CAGCCTGCCG CTGTTCCACA ATCAAGAGTC CACCGATTTC CTCAGATGGG TCTCCAAAAG 
CTGTTCAGGC GCAGCTCTGC CAATTTTAAG GATGTTATGC GGCACCGCAA TGTGTCCACA 
CTCAAGGAAC TACGTCGCAT TGTGGAGCGT TTTAACAAGG CCAAACTCAT GTCGCTGAAC 
CCCGAACTGG AGACCTCCAG CTCCGAGCCA CAGACAACTC AGCGCCACGG GCTGCGCAAG 
GAGGATTTTA AGTCTTCCAC CGATATTTAC TCTCACTCGC TGATTGCCAC CAATATGTTG 
CTGAATAGAG CCTATGGCTT TAAGGCACGC CATGTCCTGG CGCACGTGGG CTTCCTAATT 
GACAAGGATA TTGTGGAGGC CATGCAGCGA CGTTTTCACC AGCAAATTCT GGACACTGCC 
CATCAGCGCT TCCGAGCCCC AACAGATTTG CAGTACGCAT TCGCTTACTA CTCCTTCCTA 
ATGAGCGAAA CCAAAGTAAT GAGTGTAGAG GAAATCTTCG ATGAGTTCGA CACGGACGGT 
TCGGCCACCT GGTCGGATCG AGAGGTGCGA ACCTTTCTCA CTCGCATCTA TCAGCCGCCA 
CTTGACTGGT CTGCCATGCG CTACTTCGAG GAGGTGGTTC AAAACTGCAC AAGAAATCTG 
GGCATGCATT TAAAAGTGGA CACAGTCGAA CACTCCACGC TGGTCTACGA GCGATACGAG 
GACTCTAATT TGCCTACAAT AACCAGGGAT TTGGTTGTCC GATGTCCACT TCTGGCCGAA 
GCTTTGGCGG CAAACTTTGC AGTCCGACCC AAATATAACT TCCATGTCAG CCCAAAAAGA 
ACTTCGCACA GCAATTTTAT GATGCTCACA TCGAATCTCA CGGAGGTGGT GGAGTCACTG 
GACCGATTGC GTCGGAATCC GCGCAAGTTT AACTGCATCA ACGACAATCT GGATGCCAAT 
AGAGGCGAAG ACAACGAGAT GGTGCGCCAT CTGCTCGAGG ACTTTTACCT CTCCTTCTTT 
CCGAGACGCA GCAAGTTCGA GCTGCCGCCG CAATATCGTA ATCGGTTCGA GTCCTGGCGG 
GACTTTCAGC GCTGGAAGAG AAGAAAGCGG GCGGTTCTCG TGATTGGATA TGGAGTGAGT 
CTGCTGCTTG TGGTCTGTCT GCTGCGATTC ATGTGCCACC ACAAGGCGAA GCTGGTGAGG 
CGCTGTGTGC AGCGCTTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG8027 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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