Spn42Db cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spn42Db
Entrez Gene ID	35599
Full Name	Serpin 42Db
Synonyms	CG9454,Dmel\CG9454
General protein information	Preferred Names Serpin 42Db Names CG9454 gene product from transcript CG9454-RC CG9454-PC CG9454-PD Spn42Db-PC Spn42Db-PD serpin 42Db
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 42D6-42D6

mRNA and Protein(s)

mRNA	Protein	Name
NM_136400.3	NP_610244.2	serpin 42Db, isoform C
NM_001273817.1	NP_001260746.1	serpin 42Db, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Spn42Db gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spn42Db cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09070	NM_136400.3 Latest version!	Drosophila melanogaster serpin 42Db, transcript variant C (Spn42Db), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa12630	NM_001273817.1 Latest version!	Drosophila melanogaster serpin 42Db, transcript variant D (Spn42Db), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spn42Db ( NM_136400.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610244.2 Drosophila melanogaster serpin 42Db, transcript variant C (Spn42Db), mRNA.

Price & Availability↑

CloneID	OFa09070
Clone ID Related Accession (Same CDS sequence)	NM_136400.3
Accession Version	NM_136400.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	serpin 42Db, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_136400.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAGATG AGGAGTTCGC ACAGGGCTTG GAACAATTCG CTCTTTGTCT GCACGACCAT 
TTGTGCCGGG CAAGTGCTGG CCTAAACATC ATCTACTCCC CATTATCCAT CCACATCTCT 
GCGGCCATGC TGCGGATGGG AACATCCGAG GGATCGGCTA CAGCTAAGGA GATGGACGAG 
GGGCTCCGTT TCGGTGGGCT AGAAGCTCAG CAAGTAGCTG AAAGCTTTGG TGTCGTCCTC 
AAGTCCTATG AACAGTGCCA GGTTCTTAAA ATGGCAAACG GCCTATATGT TATGAAGGGT 
CTCCAAGTTG ACGAGCAGTT TGGTCACATA CTAGAGCAGA AGTTTCGCTC CAAGCCAATG 
GAGATTGACT TTGGCAGCGA GCAGGCTGCC AGCATAATCA ACAAATGGGT GGAGTCACAG 
ACCAACAACC TGATCAAGGA CATAATCGGT CCAAGAGTTC TTACCAAGGA CTCGCGGCTG 
TGCCTAGTAA ACGGTATTCA CTTCAAGGGT GAGTGGTCAA TCAGTTTCAA TGAAAAAGAA 
ACCCGCGAAG AGGATTTCTT CGGATCGGAT AGACCTACCA GAGTGAGAAT GATGCATGTG 
TGCGAGAACT TCTTTTTTGC CGTACTTCCC ATGTTCGAAG CCACTGCCCT TAGAATGAAT 
TATAGTGCCT GTAATTTGGC CATGATCATT TTACTTCCTG ATGAGAAGTC GAATCTCACG 
AGCCTCGAAA AGAAACTTAG TGATATTTCC CTAGAGGTAG TGTCATCAGC AATGAATCTG 
GAAAAAGTGG ATGTGAAGAT TCCTAGCTTT ACAGCCGAGT TCCAGCAAGA ACTATCCCAG 
GTTTTAATGC TGATGGGCAT GAACCGAATA TTCAGTGGTC AGGCAGAGCT TGGGGGAATG 
CTACAATCCG AGGAAAGCCT TTTTGTCTCC CAGATCGTCC ACAAAGCATT TATCGAGATT 
AACGAAGTGG GCACTGAAGC TGCAGCCGCA ACGGCTGCGG TTGCCACATT TAGAAGCATG 
CCTGCGCGCC AAGGACCTCC AAAGGTATTC CACGCTAATA GACCTTTCTT CTATGCTATA 
AAGGACAACA CCCATGGCCT TCTATTTGCC GGTCATTTTA TCACAACCAA GGTTGAGCAG 
TCTGAAAAAT GTAACACATG TACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610244.2
CDS	56..1222
Translation	MKDEEFAQGLEQFALCLHDHLCRASAGLNIIYSPLSIHISAAMLRMGTSEGSATAKEMDEGLRFGGLEAQQVAESFGVVLKSYEQCQVLKMANGLYVMKGLQVDEQFGHILEQKFRSKPMEIDFGSEQAASIINKWVESQTNNLIKDIIGPRVLTKDSRLCLVNGIHFKGEWSISFNEKETREEDFFGSDRPTRVRMMHVCENFFFAVLPMFEATALRMNYSACNLAMIILLPDEKSNLTSLEKKLSDISLEVVSSAMNLEKVDVKIPSFTAEFQQELSQVLMLMGMNRIFSGQAELGGMLQSEESLFVSQIVHKAFIEINEVGTEAAAATAAVATFRSMPARQGPPKVFHANRPFFYAIKDNTHGLLFAGHFITTKVEQSEKCNTCT

Target ORF information:

RefSeq Version	NM_136400.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster serpin 42Db, transcript variant C (Spn42Db), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136400.3

ORF Insert Sequence:

ATGAAAGATG AGGAGTTCGC ACAGGGCTTG GAACAATTCG CTCTTTGTCT GCACGACCAT 
TTGTGCCGGG CAAGTGCTGG CCTAAACATC ATCTACTCCC CATTATCCAT CCACATCTCT 
GCGGCCATGC TGCGGATGGG AACATCCGAG GGATCGGCTA CAGCTAAGGA GATGGACGAG 
GGGCTCCGTT TCGGTGGGCT AGAAGCTCAG CAAGTAGCTG AAAGCTTTGG TGTCGTCCTC 
AAGTCCTATG AACAGTGCCA GGTTCTTAAA ATGGCAAACG GCCTATATGT TATGAAGGGT 
CTCCAAGTTG ACGAGCAGTT TGGTCACATA CTAGAGCAGA AGTTTCGCTC CAAGCCAATG 
GAGATTGACT TTGGCAGCGA GCAGGCTGCC AGCATAATCA ACAAATGGGT GGAGTCACAG 
ACCAACAACC TGATCAAGGA CATAATCGGT CCAAGAGTTC TTACCAAGGA CTCGCGGCTG 
TGCCTAGTAA ACGGTATTCA CTTCAAGGGT GAGTGGTCAA TCAGTTTCAA TGAAAAAGAA 
ACCCGCGAAG AGGATTTCTT CGGATCGGAT AGACCTACCA GAGTGAGAAT GATGCATGTG 
TGCGAGAACT TCTTTTTTGC CGTACTTCCC ATGTTCGAAG CCACTGCCCT TAGAATGAAT 
TATAGTGCCT GTAATTTGGC CATGATCATT TTACTTCCTG ATGAGAAGTC GAATCTCACG 
AGCCTCGAAA AGAAACTTAG TGATATTTCC CTAGAGGTAG TGTCATCAGC AATGAATCTG 
GAAAAAGTGG ATGTGAAGAT TCCTAGCTTT ACAGCCGAGT TCCAGCAAGA ACTATCCCAG 
GTTTTAATGC TGATGGGCAT GAACCGAATA TTCAGTGGTC AGGCAGAGCT TGGGGGAATG 
CTACAATCCG AGGAAAGCCT TTTTGTCTCC CAGATCGTCC ACAAAGCATT TATCGAGATT 
AACGAAGTGG GCACTGAAGC TGCAGCCGCA ACGGCTGCGG TTGCCACATT TAGAAGCATG 
CCTGCGCGCC AAGGACCTCC AAAGGTATTC CACGCTAATA GACCTTTCTT CTATGCTATA 
AAGGACAACA CCCATGGCCT TCTATTTGCC GGTCATTTTA TCACAACCAA GGTTGAGCAG 
TCTGAAAAAT GTAACACATG TACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spn42Db ( NM_001273817.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260746.1 Drosophila melanogaster serpin 42Db, transcript variant D (Spn42Db), mRNA.

Price & Availability↑

CloneID	OFa12630
Clone ID Related Accession (Same CDS sequence)	NM_001273817.1
Accession Version	NM_001273817.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	serpin 42Db, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAGATG AGGAGTTCGC ACAGGGCTTG GAACAATTCG CTCTTTGTCT GCACGACCAT 
TTGTGCCGGG CAAGTGCTGG CCTAAACATC ATCTACTCCC CATTATCCAT CCACATCTCT 
GCGGCCATGC TGCGGATGGG AACATCCGAG GGATCGGCTA CAGCTAAGGA GATGGACGAG 
GGGCTCCGTT TCGGTGGGCT AGAAGCTCAG CAAGTAGCTG AAAGCTTTGG TGTCGTCCTC 
AAGTCCTATG AACAGTGCCA GGTTCTTAAA ATGGCAAACG GCCTATATGT TATGAAGGGT 
CTCCAAGTTG ACGAGCAGTT TGGTCACATA CTAGAGCAGA AGTTTCGCTC CAAGCCAATG 
GAGATTGACT TTGGCAGCGA GCAGGCTGCC AGCATAATCA ACAAATGGGT GGAGTCACAG 
ACCAACAACC TGATCAAGGA CATAATCGGT CCAAGAGTTC TTACCAAGGA CTCGCGGCTG 
TGCCTAGTAA ACGGTATTCA CTTCAAGGGT GAGTGGTCAA TCAGTTTCAA TGAAAAAGAA 
ACCCGCGAAG AGGATTTCTT CGGATCGGAT AGACCTACCA GAGTGAGAAT GATGCATGTG 
TGCGAGAACT TCTTTTTTGC CGTACTTCCC ATGTTCGAAG CCACTGCCCT TAGAATGAAT 
TATAGTGCCT GTAATTTGGC CATGATCATT TTACTTCCTG ATGAGAAGTC GAATCTCACG 
AGCCTCGAAA AGAAACTTAG TGATATTTCC CTAGAGGTAG TGTCATCAGC AATGAATCTG 
GAAAAAGTGG ATGTGAAGAT TCCTAGCTTT ACAGCCGAGT TCCAGCAAGA ACTATCCCAG 
GTTTTAATGC TGGTTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260746.1
CDS	56..916
Translation	MKDEEFAQGLEQFALCLHDHLCRASAGLNIIYSPLSIHISAAMLRMGTSEGSATAKEMDEGLRFGGLEAQQVAESFGVVLKSYEQCQVLKMANGLYVMKGLQVDEQFGHILEQKFRSKPMEIDFGSEQAASIINKWVESQTNNLIKDIIGPRVLTKDSRLCLVNGIHFKGEWSISFNEKETREEDFFGSDRPTRVRMMHVCENFFFAVLPMFEATALRMNYSACNLAMIILLPDEKSNLTSLEKKLSDISLEVVSSAMNLEKVDVKIPSFTAEFQQELSQVLMLVS

Target ORF information:

RefSeq Version	NM_001273817.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster serpin 42Db, transcript variant D (Spn42Db), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273817.1

ORF Insert Sequence:

ATGAAAGATG AGGAGTTCGC ACAGGGCTTG GAACAATTCG CTCTTTGTCT GCACGACCAT 
TTGTGCCGGG CAAGTGCTGG CCTAAACATC ATCTACTCCC CATTATCCAT CCACATCTCT 
GCGGCCATGC TGCGGATGGG AACATCCGAG GGATCGGCTA CAGCTAAGGA GATGGACGAG 
GGGCTCCGTT TCGGTGGGCT AGAAGCTCAG CAAGTAGCTG AAAGCTTTGG TGTCGTCCTC 
AAGTCCTATG AACAGTGCCA GGTTCTTAAA ATGGCAAACG GCCTATATGT TATGAAGGGT 
CTCCAAGTTG ACGAGCAGTT TGGTCACATA CTAGAGCAGA AGTTTCGCTC CAAGCCAATG 
GAGATTGACT TTGGCAGCGA GCAGGCTGCC AGCATAATCA ACAAATGGGT GGAGTCACAG 
ACCAACAACC TGATCAAGGA CATAATCGGT CCAAGAGTTC TTACCAAGGA CTCGCGGCTG 
TGCCTAGTAA ACGGTATTCA CTTCAAGGGT GAGTGGTCAA TCAGTTTCAA TGAAAAAGAA 
ACCCGCGAAG AGGATTTCTT CGGATCGGAT AGACCTACCA GAGTGAGAAT GATGCATGTG 
TGCGAGAACT TCTTTTTTGC CGTACTTCCC ATGTTCGAAG CCACTGCCCT TAGAATGAAT 
TATAGTGCCT GTAATTTGGC CATGATCATT TTACTTCCTG ATGAGAAGTC GAATCTCACG 
AGCCTCGAAA AGAAACTTAG TGATATTTCC CTAGAGGTAG TGTCATCAGC AATGAATCTG 
GAAAAAGTGG ATGTGAAGAT TCCTAGCTTT ACAGCCGAGT TCCAGCAAGA ACTATCCCAG 
GTTTTAATGC TGGTTAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Spn42Db cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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