scaf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	scaf
Entrez Gene ID	35505
Full Name	scarface
Synonyms	CG11066,CG15900,Dmel\CG11066,SPH142,scarf
General protein information	Preferred Names scarface Names CG11066 gene product from transcript CG11066-RB CG11066-PA CG11066-PB scaf-PA scaf-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 41F6-41F8

mRNA and Protein(s)

mRNA	Protein	Name
NM_136336.3	NP_610180.1	scarface, isoform B
NM_165441.2	NP_724424.1	scarface, isoform A

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	scaf	NP_724424.1
Anopheles gambiae (African malaria mosquito)	CLIPE1	XP_317365.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Here we uncover a cell non-autonomous requirement for the Epidermal growth factor receptor (Egfr) pathway in the lateral epidermis for sustained dpp expression in the LE. Specifically, we demonstrate that Egfr pathway activity in the lateral epidermis prevents expression of the gene scarface (scaf), encoding a secreted antagonist of JNK signaling
Title: Novel interplay between JNK and Egfr signaling in Drosophila dorsal closure.

Results identify the serine protease homologue-encoding gene scaf as a new transcriptional target of JNK signalling and reveal the first secreted regulator of the JNK pathway acting in a negative-feedback loop during epithelial morphogenesis.
Title: The Drosophila serine protease homologue Scarface regulates JNK signalling in a negative-feedback loop during epithelial morphogenesis.

Scarface has a key role in regulating the polarized localization of laminin A in this developmental context.
Title: Scarface, a secreted serine protease-like protein, regulates polarized localization of laminin A at the basement membrane of the Drosophila embryo.

The following scaf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the scaf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa12401	NM_136336.3 Latest version!	Drosophila melanogaster scarface (scaf), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa12401	NM_165441.2 Latest version!	Drosophila melanogaster scarface (scaf), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

scaf ( NM_136336.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610180.1 Drosophila melanogaster scarface (scaf), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa12401
Clone ID Related Accession (Same CDS sequence)	NM_136336.3 , NM_165441.2
Accession Version	NM_136336.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	scarface, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_136336.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGCTT CACACTTCAG GGAGCAGCTC GCCCTGTGCA TTACCCTGGC AGTGCTCGCC 
GCTGCCAGTG GCGACTACCG GGCCAACATG TTTCTCAATG GCCAATATCA AAACGGTATC 
AAGGATCAAA AGGAGAACAA CCTTTTAGTA AACCCCTCGA CGAACGTGTT CCTCAACCAC 
GCCATAATCA GCAGGCAAGC GTCGCCGTTC CAGGGCCCAA CCTATCTGCC GCCCAAGGAG 
TTCCTGAAGT GCGCTCCCGG CCAGCAGTGC GTCCGCAGTG GTCAGTGCCT GAACGGATAT 
TTCGCCCAGC AACTGCCCAA GATTCAAAAC TGTGATCCGG AAACCACCGT TTGCTGTACT 
TACAGGCCGC CTCCCACCAC CACAACAACA ACCACTACTT CCGTGCCAGT GGCCAATTGT 
GCCTACGACT CCGACTGTGT GACTCCGGAC AACTGCCGGA ACGGCGAAAT TAGCGCCATA 
AACTACGTAA AGAAGCAAGG ACCCAACCGC TGCCCTGCTC CTAATATATG CTGCCGCATA 
CCCTCGACCA CGCTAACGGA GGATGGGTAC ATTTTCAACC TTCCGGAAAA GACCTTCCCG 
CTGCCGACGA AACCAGCAGT GCTCGCGATG CCCTCCACCC AGGCGCCGTT CCGCCCGCAA 
CCTACGACTG CTGTGCCAGC TTCTCGACCC ACCATTGAGT ATTTGCCGCC CTCCACCACA 
CAGCATCCCA GTTACGAGAA AGTCCAGACA AGCCGACGGC CAGTCTACTT GCCACCTTCC 
CCGGCCACTG AGTCGGCCAG CAGTCTCATC CCAAAAATTA GGCCGAGGCC GGAGCCACGC 
CCCCAGCCGA CGCGCCGTCC AACGAATGAG TACCTGCCGC CTGCGGCCGC TAACGAAATC 
CCTCGCTTCG AGCCTGACCG CGCTCCGCAG CCATCAAACC AGAAGCCAAT CTACCGCGGA 
GAGGACCAGC TGTCGCCTCA GATCTTTCCC ACTCCCCAGC CAGCCAATGT TCCAAAACAC 
TTTGCCAAGT GCGCCTCAGC CTTAGTGTGC ACCTCTGAGA ACTTCTGCAA CGCCATCGGA 
GTGCTTTCTG AGACCCCCGT AGAGCTGTCT CCCATGGAAG CAGCTTTCCG AGTGCCCCTT 
ACGGACTGTT TGCAGACAGA GAACGGCTCC CCCGGAAAGT GCTGTCGTGA CCCTAACTAT 
GTAGACCCCT GGCCGGTCAA CTTAGCCGGA GTGTGCGCGA CAAGGAACAA GCGAACGAAG 
CCCACGGGAG TTAAGGATCT GGATGCAAAC TTCGCCGAAA TCCCCTGGCA AGCCATGATC 
TTGCGCGAGT CCAGTAAGAC GCTTATTTGC GGAGGTGCCA TCATCGGAGA CCAATTTGTT 
TTGAGCTCCG CCAGCTGCGT AAATGGTCTT CCGGTGACCG ACATTCGGGT CAAGGCTGGT 
GAATGGGAGC TGGGGAGCAC GAACGAGCCT CTGCCGTTCC AGCTGACTGG AGTCAAGACC 
GTCGATGTCC ATCCCGATTA CGATCCGTCG ACAAACTCCC ACGACTTGGC CATTATCCGA 
CTCGAAAGAC GTTTGGAGTT CGCATCCCAC ATCCAGCCCA TCTGCATAAG CGACGAGGAC 
CCAAAGGATT CCGAGCAATG TTTCACCTCG GGCTGGGGAA AGCAAGCGTT ATCTATACAT 
GAAGAGGGCG CTCTAATGCA CGTAACCGAC ACTTTGCCGC AAGCACGCAG CGAGTGCAGT 
GCCGACTCCA GCAGTGTGTG TAGTGCTACC AAGTTCGACT CTTGCCAATT CGACGTGGGA 
AGCGCCCTTG CCTGTGGCAG CGGCTCTAGC GTCCGCCTCA AGGGAATCTT CGCCGGTGAG 
AACTCCTGCG GAGAGGGGCA GACAGTACGT TTTGCCAAGC CGGACATTAA GTGGATTAAC 
ACTGCGTTCG CCGAAAACAA TAAGCCGCTC CTGTTGAAGC GATTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610180.1
CDS	109..2076
Translation	MSASHFREQLALCITLAVLAAASGDYRANMFLNGQYQNGIKDQKENNLLVNPSTNVFLNHAIISRQASPFQGPTYLPPKEFLKCAPGQQCVRSGQCLNGYFAQQLPKIQNCDPETTVCCTYRPPPTTTTTTTTSVPVANCAYDSDCVTPDNCRNGEISAINYVKKQGPNRCPAPNICCRIPSTTLTEDGYIFNLPEKTFPLPTKPAVLAMPSTQAPFRPQPTTAVPASRPTIEYLPPSTTQHPSYEKVQTSRRPVYLPPSPATESASSLIPKIRPRPEPRPQPTRRPTNEYLPPAAANEIPRFEPDRAPQPSNQKPIYRGEDQLSPQIFPTPQPANVPKHFAKCASALVCTSENFCNAIGVLSETPVELSPMEAAFRVPLTDCLQTENGSPGKCCRDPNYVDPWPVNLAGVCATRNKRTKPTGVKDLDANFAEIPWQAMILRESSKTLICGGAIIGDQFVLSSASCVNGLPVTDIRVKAGEWELGSTNEPLPFQLTGVKTVDVHPDYDPSTNSHDLAIIRLERRLEFASHIQPICISDEDPKDSEQCFTSGWGKQALSIHEEGALMHVTDTLPQARSECSADSSSVCSATKFDSCQFDVGSALACGSGSSVRLKGIFAGENSCGEGQTVRFAKPDIKWINTAFAENNKPLLLKRF

Target ORF information:

RefSeq Version	NM_136336.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster scarface (scaf), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136336.3

ORF Insert Sequence:

ATGTCCGCTT CACACTTCAG GGAGCAGCTC GCCCTGTGCA TTACCCTGGC AGTGCTCGCC 
GCTGCCAGTG GCGACTACCG GGCCAACATG TTTCTCAATG GCCAATATCA AAACGGTATC 
AAGGATCAAA AGGAGAACAA CCTTTTAGTA AACCCCTCGA CGAACGTGTT CCTCAACCAC 
GCCATAATCA GCAGGCAAGC GTCGCCGTTC CAGGGCCCAA CCTATCTGCC GCCCAAGGAG 
TTCCTGAAGT GCGCTCCCGG CCAGCAGTGC GTCCGCAGTG GTCAGTGCCT GAACGGATAT 
TTCGCCCAGC AACTGCCCAA GATTCAAAAC TGTGATCCGG AAACCACCGT TTGCTGTACT 
TACAGGCCGC CTCCCACCAC CACAACAACA ACCACTACTT CCGTGCCAGT GGCCAATTGT 
GCCTACGACT CCGACTGTGT GACTCCGGAC AACTGCCGGA ACGGCGAAAT TAGCGCCATA 
AACTACGTAA AGAAGCAAGG ACCCAACCGC TGCCCTGCTC CTAATATATG CTGCCGCATA 
CCCTCGACCA CGCTAACGGA GGATGGGTAC ATTTTCAACC TTCCGGAAAA GACCTTCCCG 
CTGCCGACGA AACCAGCAGT GCTCGCGATG CCCTCCACCC AGGCGCCGTT CCGCCCGCAA 
CCTACGACTG CTGTGCCAGC TTCTCGACCC ACCATTGAGT ATTTGCCGCC CTCCACCACA 
CAGCATCCCA GTTACGAGAA AGTCCAGACA AGCCGACGGC CAGTCTACTT GCCACCTTCC 
CCGGCCACTG AGTCGGCCAG CAGTCTCATC CCAAAAATTA GGCCGAGGCC GGAGCCACGC 
CCCCAGCCGA CGCGCCGTCC AACGAATGAG TACCTGCCGC CTGCGGCCGC TAACGAAATC 
CCTCGCTTCG AGCCTGACCG CGCTCCGCAG CCATCAAACC AGAAGCCAAT CTACCGCGGA 
GAGGACCAGC TGTCGCCTCA GATCTTTCCC ACTCCCCAGC CAGCCAATGT TCCAAAACAC 
TTTGCCAAGT GCGCCTCAGC CTTAGTGTGC ACCTCTGAGA ACTTCTGCAA CGCCATCGGA 
GTGCTTTCTG AGACCCCCGT AGAGCTGTCT CCCATGGAAG CAGCTTTCCG AGTGCCCCTT 
ACGGACTGTT TGCAGACAGA GAACGGCTCC CCCGGAAAGT GCTGTCGTGA CCCTAACTAT 
GTAGACCCCT GGCCGGTCAA CTTAGCCGGA GTGTGCGCGA CAAGGAACAA GCGAACGAAG 
CCCACGGGAG TTAAGGATCT GGATGCAAAC TTCGCCGAAA TCCCCTGGCA AGCCATGATC 
TTGCGCGAGT CCAGTAAGAC GCTTATTTGC GGAGGTGCCA TCATCGGAGA CCAATTTGTT 
TTGAGCTCCG CCAGCTGCGT AAATGGTCTT CCGGTGACCG ACATTCGGGT CAAGGCTGGT 
GAATGGGAGC TGGGGAGCAC GAACGAGCCT CTGCCGTTCC AGCTGACTGG AGTCAAGACC 
GTCGATGTCC ATCCCGATTA CGATCCGTCG ACAAACTCCC ACGACTTGGC CATTATCCGA 
CTCGAAAGAC GTTTGGAGTT CGCATCCCAC ATCCAGCCCA TCTGCATAAG CGACGAGGAC 
CCAAAGGATT CCGAGCAATG TTTCACCTCG GGCTGGGGAA AGCAAGCGTT ATCTATACAT 
GAAGAGGGCG CTCTAATGCA CGTAACCGAC ACTTTGCCGC AAGCACGCAG CGAGTGCAGT 
GCCGACTCCA GCAGTGTGTG TAGTGCTACC AAGTTCGACT CTTGCCAATT CGACGTGGGA 
AGCGCCCTTG CCTGTGGCAG CGGCTCTAGC GTCCGCCTCA AGGGAATCTT CGCCGGTGAG 
AACTCCTGCG GAGAGGGGCA GACAGTACGT TTTGCCAAGC CGGACATTAA GTGGATTAAC 
ACTGCGTTCG CCGAAAACAA TAAGCCGCTC CTGTTGAAGC GATTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

scaf ( NM_165441.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724424.1 Drosophila melanogaster scarface (scaf), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa12401
Clone ID Related Accession (Same CDS sequence)	NM_136336.3 , NM_165441.2
Accession Version	NM_165441.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	scarface, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165441.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGCTT CACACTTCAG GGAGCAGCTC GCCCTGTGCA TTACCCTGGC AGTGCTCGCC 
GCTGCCAGTG GCGACTACCG GGCCAACATG TTTCTCAATG GCCAATATCA AAACGGTATC 
AAGGATCAAA AGGAGAACAA CCTTTTAGTA AACCCCTCGA CGAACGTGTT CCTCAACCAC 
GCCATAATCA GCAGGCAAGC GTCGCCGTTC CAGGGCCCAA CCTATCTGCC GCCCAAGGAG 
TTCCTGAAGT GCGCTCCCGG CCAGCAGTGC GTCCGCAGTG GTCAGTGCCT GAACGGATAT 
TTCGCCCAGC AACTGCCCAA GATTCAAAAC TGTGATCCGG AAACCACCGT TTGCTGTACT 
TACAGGCCGC CTCCCACCAC CACAACAACA ACCACTACTT CCGTGCCAGT GGCCAATTGT 
GCCTACGACT CCGACTGTGT GACTCCGGAC AACTGCCGGA ACGGCGAAAT TAGCGCCATA 
AACTACGTAA AGAAGCAAGG ACCCAACCGC TGCCCTGCTC CTAATATATG CTGCCGCATA 
CCCTCGACCA CGCTAACGGA GGATGGGTAC ATTTTCAACC TTCCGGAAAA GACCTTCCCG 
CTGCCGACGA AACCAGCAGT GCTCGCGATG CCCTCCACCC AGGCGCCGTT CCGCCCGCAA 
CCTACGACTG CTGTGCCAGC TTCTCGACCC ACCATTGAGT ATTTGCCGCC CTCCACCACA 
CAGCATCCCA GTTACGAGAA AGTCCAGACA AGCCGACGGC CAGTCTACTT GCCACCTTCC 
CCGGCCACTG AGTCGGCCAG CAGTCTCATC CCAAAAATTA GGCCGAGGCC GGAGCCACGC 
CCCCAGCCGA CGCGCCGTCC AACGAATGAG TACCTGCCGC CTGCGGCCGC TAACGAAATC 
CCTCGCTTCG AGCCTGACCG CGCTCCGCAG CCATCAAACC AGAAGCCAAT CTACCGCGGA 
GAGGACCAGC TGTCGCCTCA GATCTTTCCC ACTCCCCAGC CAGCCAATGT TCCAAAACAC 
TTTGCCAAGT GCGCCTCAGC CTTAGTGTGC ACCTCTGAGA ACTTCTGCAA CGCCATCGGA 
GTGCTTTCTG AGACCCCCGT AGAGCTGTCT CCCATGGAAG CAGCTTTCCG AGTGCCCCTT 
ACGGACTGTT TGCAGACAGA GAACGGCTCC CCCGGAAAGT GCTGTCGTGA CCCTAACTAT 
GTAGACCCCT GGCCGGTCAA CTTAGCCGGA GTGTGCGCGA CAAGGAACAA GCGAACGAAG 
CCCACGGGAG TTAAGGATCT GGATGCAAAC TTCGCCGAAA TCCCCTGGCA AGCCATGATC 
TTGCGCGAGT CCAGTAAGAC GCTTATTTGC GGAGGTGCCA TCATCGGAGA CCAATTTGTT 
TTGAGCTCCG CCAGCTGCGT AAATGGTCTT CCGGTGACCG ACATTCGGGT CAAGGCTGGT 
GAATGGGAGC TGGGGAGCAC GAACGAGCCT CTGCCGTTCC AGCTGACTGG AGTCAAGACC 
GTCGATGTCC ATCCCGATTA CGATCCGTCG ACAAACTCCC ACGACTTGGC CATTATCCGA 
CTCGAAAGAC GTTTGGAGTT CGCATCCCAC ATCCAGCCCA TCTGCATAAG CGACGAGGAC 
CCAAAGGATT CCGAGCAATG TTTCACCTCG GGCTGGGGAA AGCAAGCGTT ATCTATACAT 
GAAGAGGGCG CTCTAATGCA CGTAACCGAC ACTTTGCCGC AAGCACGCAG CGAGTGCAGT 
GCCGACTCCA GCAGTGTGTG TAGTGCTACC AAGTTCGACT CTTGCCAATT CGACGTGGGA 
AGCGCCCTTG CCTGTGGCAG CGGCTCTAGC GTCCGCCTCA AGGGAATCTT CGCCGGTGAG 
AACTCCTGCG GAGAGGGGCA GACAGTACGT TTTGCCAAGC CGGACATTAA GTGGATTAAC 
ACTGCGTTCG CCGAAAACAA TAAGCCGCTC CTGTTGAAGC GATTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724424.1
CDS	456..2423
Translation	MSASHFREQLALCITLAVLAAASGDYRANMFLNGQYQNGIKDQKENNLLVNPSTNVFLNHAIISRQASPFQGPTYLPPKEFLKCAPGQQCVRSGQCLNGYFAQQLPKIQNCDPETTVCCTYRPPPTTTTTTTTSVPVANCAYDSDCVTPDNCRNGEISAINYVKKQGPNRCPAPNICCRIPSTTLTEDGYIFNLPEKTFPLPTKPAVLAMPSTQAPFRPQPTTAVPASRPTIEYLPPSTTQHPSYEKVQTSRRPVYLPPSPATESASSLIPKIRPRPEPRPQPTRRPTNEYLPPAAANEIPRFEPDRAPQPSNQKPIYRGEDQLSPQIFPTPQPANVPKHFAKCASALVCTSENFCNAIGVLSETPVELSPMEAAFRVPLTDCLQTENGSPGKCCRDPNYVDPWPVNLAGVCATRNKRTKPTGVKDLDANFAEIPWQAMILRESSKTLICGGAIIGDQFVLSSASCVNGLPVTDIRVKAGEWELGSTNEPLPFQLTGVKTVDVHPDYDPSTNSHDLAIIRLERRLEFASHIQPICISDEDPKDSEQCFTSGWGKQALSIHEEGALMHVTDTLPQARSECSADSSSVCSATKFDSCQFDVGSALACGSGSSVRLKGIFAGENSCGEGQTVRFAKPDIKWINTAFAENNKPLLLKRF

Target ORF information:

RefSeq Version	NM_165441.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster scarface (scaf), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165441.2

ORF Insert Sequence:

ATGTCCGCTT CACACTTCAG GGAGCAGCTC GCCCTGTGCA TTACCCTGGC AGTGCTCGCC 
GCTGCCAGTG GCGACTACCG GGCCAACATG TTTCTCAATG GCCAATATCA AAACGGTATC 
AAGGATCAAA AGGAGAACAA CCTTTTAGTA AACCCCTCGA CGAACGTGTT CCTCAACCAC 
GCCATAATCA GCAGGCAAGC GTCGCCGTTC CAGGGCCCAA CCTATCTGCC GCCCAAGGAG 
TTCCTGAAGT GCGCTCCCGG CCAGCAGTGC GTCCGCAGTG GTCAGTGCCT GAACGGATAT 
TTCGCCCAGC AACTGCCCAA GATTCAAAAC TGTGATCCGG AAACCACCGT TTGCTGTACT 
TACAGGCCGC CTCCCACCAC CACAACAACA ACCACTACTT CCGTGCCAGT GGCCAATTGT 
GCCTACGACT CCGACTGTGT GACTCCGGAC AACTGCCGGA ACGGCGAAAT TAGCGCCATA 
AACTACGTAA AGAAGCAAGG ACCCAACCGC TGCCCTGCTC CTAATATATG CTGCCGCATA 
CCCTCGACCA CGCTAACGGA GGATGGGTAC ATTTTCAACC TTCCGGAAAA GACCTTCCCG 
CTGCCGACGA AACCAGCAGT GCTCGCGATG CCCTCCACCC AGGCGCCGTT CCGCCCGCAA 
CCTACGACTG CTGTGCCAGC TTCTCGACCC ACCATTGAGT ATTTGCCGCC CTCCACCACA 
CAGCATCCCA GTTACGAGAA AGTCCAGACA AGCCGACGGC CAGTCTACTT GCCACCTTCC 
CCGGCCACTG AGTCGGCCAG CAGTCTCATC CCAAAAATTA GGCCGAGGCC GGAGCCACGC 
CCCCAGCCGA CGCGCCGTCC AACGAATGAG TACCTGCCGC CTGCGGCCGC TAACGAAATC 
CCTCGCTTCG AGCCTGACCG CGCTCCGCAG CCATCAAACC AGAAGCCAAT CTACCGCGGA 
GAGGACCAGC TGTCGCCTCA GATCTTTCCC ACTCCCCAGC CAGCCAATGT TCCAAAACAC 
TTTGCCAAGT GCGCCTCAGC CTTAGTGTGC ACCTCTGAGA ACTTCTGCAA CGCCATCGGA 
GTGCTTTCTG AGACCCCCGT AGAGCTGTCT CCCATGGAAG CAGCTTTCCG AGTGCCCCTT 
ACGGACTGTT TGCAGACAGA GAACGGCTCC CCCGGAAAGT GCTGTCGTGA CCCTAACTAT 
GTAGACCCCT GGCCGGTCAA CTTAGCCGGA GTGTGCGCGA CAAGGAACAA GCGAACGAAG 
CCCACGGGAG TTAAGGATCT GGATGCAAAC TTCGCCGAAA TCCCCTGGCA AGCCATGATC 
TTGCGCGAGT CCAGTAAGAC GCTTATTTGC GGAGGTGCCA TCATCGGAGA CCAATTTGTT 
TTGAGCTCCG CCAGCTGCGT AAATGGTCTT CCGGTGACCG ACATTCGGGT CAAGGCTGGT 
GAATGGGAGC TGGGGAGCAC GAACGAGCCT CTGCCGTTCC AGCTGACTGG AGTCAAGACC 
GTCGATGTCC ATCCCGATTA CGATCCGTCG ACAAACTCCC ACGACTTGGC CATTATCCGA 
CTCGAAAGAC GTTTGGAGTT CGCATCCCAC ATCCAGCCCA TCTGCATAAG CGACGAGGAC 
CCAAAGGATT CCGAGCAATG TTTCACCTCG GGCTGGGGAA AGCAAGCGTT ATCTATACAT 
GAAGAGGGCG CTCTAATGCA CGTAACCGAC ACTTTGCCGC AAGCACGCAG CGAGTGCAGT 
GCCGACTCCA GCAGTGTGTG TAGTGCTACC AAGTTCGACT CTTGCCAATT CGACGTGGGA 
AGCGCCCTTG CCTGTGGCAG CGGCTCTAGC GTCCGCCTCA AGGGAATCTT CGCCGGTGAG 
AACTCCTGCG GAGAGGGGCA GACAGTACGT TTTGCCAAGC CGGACATTAA GTGGATTAAC 
ACTGCGTTCG CCGAAAACAA TAAGCCGCTC CTGTTGAAGC GATTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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scaf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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