CG31612 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG31612
Entrez Gene ID	35427
Full Name	CG31612 gene product from transcript CG31612-RA
Synonyms	CG11631,CG11632,Dmel\CG31612
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 40A2-40A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_165400.3	NP_724363.1	uncharacterized protein, isoform A
NM_001169571.2	NP_001163042.1	uncharacterized protein, isoform B

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009025	XP_319775.4
Drosophila melanogaster (fruit fly)	CG31612	NP_724363.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG31612 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG31612 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa15150	NM_165400.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG31612), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa15150	NM_001169571.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG31612), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG31612 ( NM_165400.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724363.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG31612), mRNA.

Price & Availability↑

CloneID	OFa15150
Clone ID Related Accession (Same CDS sequence)	NM_165400.3 , NM_001169571.2
Accession Version	NM_165400.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165400.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACGAAG ACCATCTCGA GGACACTCAT CTGTGCATCA GGTGCAACGC AACTATACTT 
GGACTCTCAA AATATATTGA TCATCGAAAA CGGAACTGTC TTATAACAGG AAAGCAAAAT 
GCTTCGCCAA AAACCGATGG ACGAACAAGA ATCTCTGCTG ACACTCATGT TTCGGCGACG 
CTTATCAGTA GCAGTAATTT GGATCATTCA TATGATGGCT TCCATTTCAC GGAACCGGAG 
GCTCCAAGTA GTTACCTTCG CAAAACTGCC GCCAGTCATG GCGGTAAGAC CTCAAAGTCC 
TTGACTGAAG CCTATGATCT CCCATACGAA CTTGGAGCCG ATCTCTTCTT TTCGTCCCTA 
CAACTGCAGA GCGTCGCCAC GGGTGGGAAA ATAGGATCGG TACCCCGTCA AGAGCGAAGC 
AAAGATGAGT CCTGGTCCGC GCCAGCTGGC GATCCCCTAC TGAAAGCAGT GAGGGAGAAC 
GATGAAACTG TCTTTAAGCC GCTTCACTTT GACCATGAGT ACCCAGAAGC GAGTGATGAG 
GAGGATGATG AGGATGAACA CGAGGAATTT GATGCGGAAG AAGATGAGGA AGAGTACGAT 
GCAAGGCGTC ACTCACCGCC CATTGTGCCC GCCAGTCACA CAGGTGGTAA GTGGAAACCA 
GAACAGCGGC CGCAGCTACG ACATCCGCAT ATAGAACGCC TCTCGCCAAG TTGGGATGAG 
CCCCCCGAAG AAAACTACAC TCACCCTCCA GCGGACCATA CCAAGGGCAA ATGGGTTCCG 
GGTAGCAAGC AGTTGGAGTA CCGCGAAAAT TTGGATCTTA CCAAGCTGGA CCAACCGGGA 
GCTAGTTATT GGTGCAACAT TTGCTGCAGG CGGCTGAAAA GTCGCTTATA TTATAACCAA 
CATTTGAGGA GTGGCTATCA CATCAAAAGA GCTGAAGCAG AGTGCGAATT AGAGCAGGCT 
ACTCTGGGCA GGGAACTTAC CCTAAGCAAG GACTTCTCAA TAAAAGACGA TGCGGACAAA 
AATGAACCAA AGCCACCGAA ACGACAGAGG AGAGCAAATC TTCTGCGATG CGATCTATGC 
CGGCACACAA TGGCAAGGCA TTTGATTGGC AAACATTTAA TTTCCCATTA TCATTTCAGA 
AGACTCCAAC AGCAGAGTCG AATTAGAAGA CAAGCTTGCT TGCAGGAAAT ACTTAAGCAC 
ATGGGAAGCA TAGTGAGGCA ATCTCCATTT CAATGCATGC CTTGTCGATT CTATGCCAAC 
ACTGAGGAGA CCTTTCTTTA TCATTGGAAA TCCCACGAGC ACTTGGAGTT GACCAAGAGA 
CTGGGGGGGA CTTTTTGGTG TTCCTTTTGC CAGTTTAACT CGAACACCAA TAATTCCATG 
TTGCTGCATT TGTTGGATTC CTCCCATAAG GAGGTGCTTT TAGCCCTTAA TAGATCAGTT 
CCCATATGTA TCGCCCAAAG AAGACGGATC CAGTGCTCTA GGTGTGGAAT GGAATTTTTG 
TACAACATCC AACTACGTCA ACACTCGATC ACTGAGCATC CAGGACTTGC TCTTACAGGG 
ACTGCAGCCG ATGAATACCA GAGTAGGTTT CGATGCAATT TGTGTGGATC CTCACAGAAA 
TCTCGCTTAG CTCTTCAACG CCATAAAAAG CACAAACACC ACTTAGCAAG GTATTTTTGT 
GCCATTTGCC GTTTGGAATT CGACAACTCT TTGGATGCGC GTCGTCATAG AAGCTTGGTG 
ATACATAAGC AAAAAGCCCG ACCCCAAACG TCTATTCCAC AACCCGAAAA CGAAATAGAA 
CATATGCTAA GAGAAGTCCT AGAGGAGACA GTTCCTAGTC CACCCGCCAA AAGAAGTGCG 
GATGTTAACG CAAAGTGCTC GAACAGCAGA AAAACGATTG AATCGTCCCA AAGATTAGCC 
CAACATCAAG CAGAAGTTCA TTCCTCGGAT AATCATTTGT GTTTGAGCTG TGGAATAAGC 
TTTGAGTCAG CTCAGGCACT CGGACGACAC ACCCGCAGCT GTCAACCCCT GGCCAGCACA 
TCAACCCCCG CTGATCTATC ACAAGCTCAA AAGAAATCAA TGTACTCCTG CGATCAATGT 
CGCTTCCAAT CTCAATATGA GTCCGATCTA GTTTATCACC GCATTTTTCA CACACGATCC 
GGAACAATAG GGAAAAACGA GGTTCTTCAG TGCCCTTTGT GCCCGAAGAA TTTCAAAAAG 
CACTCGCTTA GGGCTCACCT AAGGAACCAT ACAAATGAAA AAATTTTTGA ATGCACTGAG 
TGCCTTCAGA AATTTGCTAG AAGACACAAC CTTAAGAACC ACGTAATCAC AAAGCATGCC 
AAAGTAGGCG ACAAGGAGAA GAAAAAATAT GCTGCGAAAG ATGTTGAACA ATCAAAACCC 
AAATATCAGT GTGGGACATG TGGAAAATTA CTGGCCAAAA AGTATTCCCT TAAACTTCAT 
GAGATTAGCC ACTCGAAGGC AGTTGAGCGC AATTATCTCT GCCATTTTGC GGACTGCTCA 
TATGCAGGGC GAACCCCGGA AAGCCTGAAA ACCCACTTGG TCTCCCACTC CCAGGAAAAT 
CACAAATGTG CGCGGATTAA CTGCAGCTAT GTGGGAAAGA GCGAGCTGCA CCTAAAAAGG 
CACCTCAAGT CGGCTCATTC CACGGAGAAG AATGGTGAGG AGTGGTTTTC GTGCGATCAA 
TGCGATTTCA GAGCCCGCAT CAAGGGTCAC CTCCGTCGGC ACTCGTTGCG CCACTCGGGC 
CAGAAGCCCC ACCAATGCCC CCACTGCGAC TTCCAGTGCA GCACCATAGA TAACCTACGT 
AAGCACATCA TCAAGACGGG AAAGCATCCC GGCATGTTCA TTTACCACTG CGCTAAGTGC 
TCCGATGAAG AAGCCGGTGA AATATTTAAG AGCAACAGCT ACAAAGAATA CCAGCACCAC 
TTGAAGACTC ACAAGGCTGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724363.1
CDS	61..3024
Translation	MNEDHLEDTHLCIRCNATILGLSKYIDHRKRNCLITGKQNASPKTDGRTRISADTHVSATLISSSNLDHSYDGFHFTEPEAPSSYLRKTAASHGGKTSKSLTEAYDLPYELGADLFFSSLQLQSVATGGKIGSVPRQERSKDESWSAPAGDPLLKAVRENDETVFKPLHFDHEYPEASDEEDDEDEHEEFDAEEDEEEYDARRHSPPIVPASHTGGKWKPEQRPQLRHPHIERLSPSWDEPPEENYTHPPADHTKGKWVPGSKQLEYRENLDLTKLDQPGASYWCNICCRRLKSRLYYNQHLRSGYHIKRAEAECELEQATLGRELTLSKDFSIKDDADKNEPKPPKRQRRANLLRCDLCRHTMARHLIGKHLISHYHFRRLQQQSRIRRQACLQEILKHMGSIVRQSPFQCMPCRFYANTEETFLYHWKSHEHLELTKRLGGTFWCSFCQFNSNTNNSMLLHLLDSSHKEVLLALNRSVPICIAQRRRIQCSRCGMEFLYNIQLRQHSITEHPGLALTGTAADEYQSRFRCNLCGSSQKSRLALQRHKKHKHHLARYFCAICRLEFDNSLDARRHRSLVIHKQKARPQTSIPQPENEIEHMLREVLEETVPSPPAKRSADVNAKCSNSRKTIESSQRLAQHQAEVHSSDNHLCLSCGISFESAQALGRHTRSCQPLASTSTPADLSQAQKKSMYSCDQCRFQSQYESDLVYHRIFHTRSGTIGKNEVLQCPLCPKNFKKHSLRAHLRNHTNEKIFECTECLQKFARRHNLKNHVITKHAKVGDKEKKKYAAKDVEQSKPKYQCGTCGKLLAKKYSLKLHEISHSKAVERNYLCHFADCSYAGRTPESLKTHLVSHSQENHKCARINCSYVGKSELHLKRHLKSAHSTEKNGEEWFSCDQCDFRARIKGHLRRHSLRHSGQKPHQCPHCDFQCSTIDNLRKHIIKTGKHPGMFIYHCAKCSDEEAGEIFKSNSYKEYQHHLKTHKAG

Target ORF information:

RefSeq Version	NM_165400.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG31612), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165400.3

ORF Insert Sequence:

ATGAACGAAG ACCATCTCGA GGACACTCAT CTGTGCATCA GGTGCAACGC AACTATACTT 
GGACTCTCAA AATATATTGA TCATCGAAAA CGGAACTGTC TTATAACAGG AAAGCAAAAT 
GCTTCGCCAA AAACCGATGG ACGAACAAGA ATCTCTGCTG ACACTCATGT TTCGGCGACG 
CTTATCAGTA GCAGTAATTT GGATCATTCA TATGATGGCT TCCATTTCAC GGAACCGGAG 
GCTCCAAGTA GTTACCTTCG CAAAACTGCC GCCAGTCATG GCGGTAAGAC CTCAAAGTCC 
TTGACTGAAG CCTATGATCT CCCATACGAA CTTGGAGCCG ATCTCTTCTT TTCGTCCCTA 
CAACTGCAGA GCGTCGCCAC GGGTGGGAAA ATAGGATCGG TACCCCGTCA AGAGCGAAGC 
AAAGATGAGT CCTGGTCCGC GCCAGCTGGC GATCCCCTAC TGAAAGCAGT GAGGGAGAAC 
GATGAAACTG TCTTTAAGCC GCTTCACTTT GACCATGAGT ACCCAGAAGC GAGTGATGAG 
GAGGATGATG AGGATGAACA CGAGGAATTT GATGCGGAAG AAGATGAGGA AGAGTACGAT 
GCAAGGCGTC ACTCACCGCC CATTGTGCCC GCCAGTCACA CAGGTGGTAA GTGGAAACCA 
GAACAGCGGC CGCAGCTACG ACATCCGCAT ATAGAACGCC TCTCGCCAAG TTGGGATGAG 
CCCCCCGAAG AAAACTACAC TCACCCTCCA GCGGACCATA CCAAGGGCAA ATGGGTTCCG 
GGTAGCAAGC AGTTGGAGTA CCGCGAAAAT TTGGATCTTA CCAAGCTGGA CCAACCGGGA 
GCTAGTTATT GGTGCAACAT TTGCTGCAGG CGGCTGAAAA GTCGCTTATA TTATAACCAA 
CATTTGAGGA GTGGCTATCA CATCAAAAGA GCTGAAGCAG AGTGCGAATT AGAGCAGGCT 
ACTCTGGGCA GGGAACTTAC CCTAAGCAAG GACTTCTCAA TAAAAGACGA TGCGGACAAA 
AATGAACCAA AGCCACCGAA ACGACAGAGG AGAGCAAATC TTCTGCGATG CGATCTATGC 
CGGCACACAA TGGCAAGGCA TTTGATTGGC AAACATTTAA TTTCCCATTA TCATTTCAGA 
AGACTCCAAC AGCAGAGTCG AATTAGAAGA CAAGCTTGCT TGCAGGAAAT ACTTAAGCAC 
ATGGGAAGCA TAGTGAGGCA ATCTCCATTT CAATGCATGC CTTGTCGATT CTATGCCAAC 
ACTGAGGAGA CCTTTCTTTA TCATTGGAAA TCCCACGAGC ACTTGGAGTT GACCAAGAGA 
CTGGGGGGGA CTTTTTGGTG TTCCTTTTGC CAGTTTAACT CGAACACCAA TAATTCCATG 
TTGCTGCATT TGTTGGATTC CTCCCATAAG GAGGTGCTTT TAGCCCTTAA TAGATCAGTT 
CCCATATGTA TCGCCCAAAG AAGACGGATC CAGTGCTCTA GGTGTGGAAT GGAATTTTTG 
TACAACATCC AACTACGTCA ACACTCGATC ACTGAGCATC CAGGACTTGC TCTTACAGGG 
ACTGCAGCCG ATGAATACCA GAGTAGGTTT CGATGCAATT TGTGTGGATC CTCACAGAAA 
TCTCGCTTAG CTCTTCAACG CCATAAAAAG CACAAACACC ACTTAGCAAG GTATTTTTGT 
GCCATTTGCC GTTTGGAATT CGACAACTCT TTGGATGCGC GTCGTCATAG AAGCTTGGTG 
ATACATAAGC AAAAAGCCCG ACCCCAAACG TCTATTCCAC AACCCGAAAA CGAAATAGAA 
CATATGCTAA GAGAAGTCCT AGAGGAGACA GTTCCTAGTC CACCCGCCAA AAGAAGTGCG 
GATGTTAACG CAAAGTGCTC GAACAGCAGA AAAACGATTG AATCGTCCCA AAGATTAGCC 
CAACATCAAG CAGAAGTTCA TTCCTCGGAT AATCATTTGT GTTTGAGCTG TGGAATAAGC 
TTTGAGTCAG CTCAGGCACT CGGACGACAC ACCCGCAGCT GTCAACCCCT GGCCAGCACA 
TCAACCCCCG CTGATCTATC ACAAGCTCAA AAGAAATCAA TGTACTCCTG CGATCAATGT 
CGCTTCCAAT CTCAATATGA GTCCGATCTA GTTTATCACC GCATTTTTCA CACACGATCC 
GGAACAATAG GGAAAAACGA GGTTCTTCAG TGCCCTTTGT GCCCGAAGAA TTTCAAAAAG 
CACTCGCTTA GGGCTCACCT AAGGAACCAT ACAAATGAAA AAATTTTTGA ATGCACTGAG 
TGCCTTCAGA AATTTGCTAG AAGACACAAC CTTAAGAACC ACGTAATCAC AAAGCATGCC 
AAAGTAGGCG ACAAGGAGAA GAAAAAATAT GCTGCGAAAG ATGTTGAACA ATCAAAACCC 
AAATATCAGT GTGGGACATG TGGAAAATTA CTGGCCAAAA AGTATTCCCT TAAACTTCAT 
GAGATTAGCC ACTCGAAGGC AGTTGAGCGC AATTATCTCT GCCATTTTGC GGACTGCTCA 
TATGCAGGGC GAACCCCGGA AAGCCTGAAA ACCCACTTGG TCTCCCACTC CCAGGAAAAT 
CACAAATGTG CGCGGATTAA CTGCAGCTAT GTGGGAAAGA GCGAGCTGCA CCTAAAAAGG 
CACCTCAAGT CGGCTCATTC CACGGAGAAG AATGGTGAGG AGTGGTTTTC GTGCGATCAA 
TGCGATTTCA GAGCCCGCAT CAAGGGTCAC CTCCGTCGGC ACTCGTTGCG CCACTCGGGC 
CAGAAGCCCC ACCAATGCCC CCACTGCGAC TTCCAGTGCA GCACCATAGA TAACCTACGT 
AAGCACATCA TCAAGACGGG AAAGCATCCC GGCATGTTCA TTTACCACTG CGCTAAGTGC 
TCCGATGAAG AAGCCGGTGA AATATTTAAG AGCAACAGCT ACAAAGAATA CCAGCACCAC 
TTGAAGACTC ACAAGGCTGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG31612 ( NM_001169571.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163042.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG31612), mRNA.

Price & Availability↑

CloneID	OFa15150
Clone ID Related Accession (Same CDS sequence)	NM_165400.3 , NM_001169571.2
Accession Version	NM_001169571.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001169571.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACGAAG ACCATCTCGA GGACACTCAT CTGTGCATCA GGTGCAACGC AACTATACTT 
GGACTCTCAA AATATATTGA TCATCGAAAA CGGAACTGTC TTATAACAGG AAAGCAAAAT 
GCTTCGCCAA AAACCGATGG ACGAACAAGA ATCTCTGCTG ACACTCATGT TTCGGCGACG 
CTTATCAGTA GCAGTAATTT GGATCATTCA TATGATGGCT TCCATTTCAC GGAACCGGAG 
GCTCCAAGTA GTTACCTTCG CAAAACTGCC GCCAGTCATG GCGGTAAGAC CTCAAAGTCC 
TTGACTGAAG CCTATGATCT CCCATACGAA CTTGGAGCCG ATCTCTTCTT TTCGTCCCTA 
CAACTGCAGA GCGTCGCCAC GGGTGGGAAA ATAGGATCGG TACCCCGTCA AGAGCGAAGC 
AAAGATGAGT CCTGGTCCGC GCCAGCTGGC GATCCCCTAC TGAAAGCAGT GAGGGAGAAC 
GATGAAACTG TCTTTAAGCC GCTTCACTTT GACCATGAGT ACCCAGAAGC GAGTGATGAG 
GAGGATGATG AGGATGAACA CGAGGAATTT GATGCGGAAG AAGATGAGGA AGAGTACGAT 
GCAAGGCGTC ACTCACCGCC CATTGTGCCC GCCAGTCACA CAGGTGGTAA GTGGAAACCA 
GAACAGCGGC CGCAGCTACG ACATCCGCAT ATAGAACGCC TCTCGCCAAG TTGGGATGAG 
CCCCCCGAAG AAAACTACAC TCACCCTCCA GCGGACCATA CCAAGGGCAA ATGGGTTCCG 
GGTAGCAAGC AGTTGGAGTA CCGCGAAAAT TTGGATCTTA CCAAGCTGGA CCAACCGGGA 
GCTAGTTATT GGTGCAACAT TTGCTGCAGG CGGCTGAAAA GTCGCTTATA TTATAACCAA 
CATTTGAGGA GTGGCTATCA CATCAAAAGA GCTGAAGCAG AGTGCGAATT AGAGCAGGCT 
ACTCTGGGCA GGGAACTTAC CCTAAGCAAG GACTTCTCAA TAAAAGACGA TGCGGACAAA 
AATGAACCAA AGCCACCGAA ACGACAGAGG AGAGCAAATC TTCTGCGATG CGATCTATGC 
CGGCACACAA TGGCAAGGCA TTTGATTGGC AAACATTTAA TTTCCCATTA TCATTTCAGA 
AGACTCCAAC AGCAGAGTCG AATTAGAAGA CAAGCTTGCT TGCAGGAAAT ACTTAAGCAC 
ATGGGAAGCA TAGTGAGGCA ATCTCCATTT CAATGCATGC CTTGTCGATT CTATGCCAAC 
ACTGAGGAGA CCTTTCTTTA TCATTGGAAA TCCCACGAGC ACTTGGAGTT GACCAAGAGA 
CTGGGGGGGA CTTTTTGGTG TTCCTTTTGC CAGTTTAACT CGAACACCAA TAATTCCATG 
TTGCTGCATT TGTTGGATTC CTCCCATAAG GAGGTGCTTT TAGCCCTTAA TAGATCAGTT 
CCCATATGTA TCGCCCAAAG AAGACGGATC CAGTGCTCTA GGTGTGGAAT GGAATTTTTG 
TACAACATCC AACTACGTCA ACACTCGATC ACTGAGCATC CAGGACTTGC TCTTACAGGG 
ACTGCAGCCG ATGAATACCA GAGTAGGTTT CGATGCAATT TGTGTGGATC CTCACAGAAA 
TCTCGCTTAG CTCTTCAACG CCATAAAAAG CACAAACACC ACTTAGCAAG GTATTTTTGT 
GCCATTTGCC GTTTGGAATT CGACAACTCT TTGGATGCGC GTCGTCATAG AAGCTTGGTG 
ATACATAAGC AAAAAGCCCG ACCCCAAACG TCTATTCCAC AACCCGAAAA CGAAATAGAA 
CATATGCTAA GAGAAGTCCT AGAGGAGACA GTTCCTAGTC CACCCGCCAA AAGAAGTGCG 
GATGTTAACG CAAAGTGCTC GAACAGCAGA AAAACGATTG AATCGTCCCA AAGATTAGCC 
CAACATCAAG CAGAAGTTCA TTCCTCGGAT AATCATTTGT GTTTGAGCTG TGGAATAAGC 
TTTGAGTCAG CTCAGGCACT CGGACGACAC ACCCGCAGCT GTCAACCCCT GGCCAGCACA 
TCAACCCCCG CTGATCTATC ACAAGCTCAA AAGAAATCAA TGTACTCCTG CGATCAATGT 
CGCTTCCAAT CTCAATATGA GTCCGATCTA GTTTATCACC GCATTTTTCA CACACGATCC 
GGAACAATAG GGAAAAACGA GGTTCTTCAG TGCCCTTTGT GCCCGAAGAA TTTCAAAAAG 
CACTCGCTTA GGGCTCACCT AAGGAACCAT ACAAATGAAA AAATTTTTGA ATGCACTGAG 
TGCCTTCAGA AATTTGCTAG AAGACACAAC CTTAAGAACC ACGTAATCAC AAAGCATGCC 
AAAGTAGGCG ACAAGGAGAA GAAAAAATAT GCTGCGAAAG ATGTTGAACA ATCAAAACCC 
AAATATCAGT GTGGGACATG TGGAAAATTA CTGGCCAAAA AGTATTCCCT TAAACTTCAT 
GAGATTAGCC ACTCGAAGGC AGTTGAGCGC AATTATCTCT GCCATTTTGC GGACTGCTCA 
TATGCAGGGC GAACCCCGGA AAGCCTGAAA ACCCACTTGG TCTCCCACTC CCAGGAAAAT 
CACAAATGTG CGCGGATTAA CTGCAGCTAT GTGGGAAAGA GCGAGCTGCA CCTAAAAAGG 
CACCTCAAGT CGGCTCATTC CACGGAGAAG AATGGTGAGG AGTGGTTTTC GTGCGATCAA 
TGCGATTTCA GAGCCCGCAT CAAGGGTCAC CTCCGTCGGC ACTCGTTGCG CCACTCGGGC 
CAGAAGCCCC ACCAATGCCC CCACTGCGAC TTCCAGTGCA GCACCATAGA TAACCTACGT 
AAGCACATCA TCAAGACGGG AAAGCATCCC GGCATGTTCA TTTACCACTG CGCTAAGTGC 
TCCGATGAAG AAGCCGGTGA AATATTTAAG AGCAACAGCT ACAAAGAATA CCAGCACCAC 
TTGAAGACTC ACAAGGCTGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163042.1
CDS	209..3172
Translation	MNEDHLEDTHLCIRCNATILGLSKYIDHRKRNCLITGKQNASPKTDGRTRISADTHVSATLISSSNLDHSYDGFHFTEPEAPSSYLRKTAASHGGKTSKSLTEAYDLPYELGADLFFSSLQLQSVATGGKIGSVPRQERSKDESWSAPAGDPLLKAVRENDETVFKPLHFDHEYPEASDEEDDEDEHEEFDAEEDEEEYDARRHSPPIVPASHTGGKWKPEQRPQLRHPHIERLSPSWDEPPEENYTHPPADHTKGKWVPGSKQLEYRENLDLTKLDQPGASYWCNICCRRLKSRLYYNQHLRSGYHIKRAEAECELEQATLGRELTLSKDFSIKDDADKNEPKPPKRQRRANLLRCDLCRHTMARHLIGKHLISHYHFRRLQQQSRIRRQACLQEILKHMGSIVRQSPFQCMPCRFYANTEETFLYHWKSHEHLELTKRLGGTFWCSFCQFNSNTNNSMLLHLLDSSHKEVLLALNRSVPICIAQRRRIQCSRCGMEFLYNIQLRQHSITEHPGLALTGTAADEYQSRFRCNLCGSSQKSRLALQRHKKHKHHLARYFCAICRLEFDNSLDARRHRSLVIHKQKARPQTSIPQPENEIEHMLREVLEETVPSPPAKRSADVNAKCSNSRKTIESSQRLAQHQAEVHSSDNHLCLSCGISFESAQALGRHTRSCQPLASTSTPADLSQAQKKSMYSCDQCRFQSQYESDLVYHRIFHTRSGTIGKNEVLQCPLCPKNFKKHSLRAHLRNHTNEKIFECTECLQKFARRHNLKNHVITKHAKVGDKEKKKYAAKDVEQSKPKYQCGTCGKLLAKKYSLKLHEISHSKAVERNYLCHFADCSYAGRTPESLKTHLVSHSQENHKCARINCSYVGKSELHLKRHLKSAHSTEKNGEEWFSCDQCDFRARIKGHLRRHSLRHSGQKPHQCPHCDFQCSTIDNLRKHIIKTGKHPGMFIYHCAKCSDEEAGEIFKSNSYKEYQHHLKTHKAG

Target ORF information:

RefSeq Version	NM_001169571.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG31612), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169571.2

ORF Insert Sequence:

ATGAACGAAG ACCATCTCGA GGACACTCAT CTGTGCATCA GGTGCAACGC AACTATACTT 
GGACTCTCAA AATATATTGA TCATCGAAAA CGGAACTGTC TTATAACAGG AAAGCAAAAT 
GCTTCGCCAA AAACCGATGG ACGAACAAGA ATCTCTGCTG ACACTCATGT TTCGGCGACG 
CTTATCAGTA GCAGTAATTT GGATCATTCA TATGATGGCT TCCATTTCAC GGAACCGGAG 
GCTCCAAGTA GTTACCTTCG CAAAACTGCC GCCAGTCATG GCGGTAAGAC CTCAAAGTCC 
TTGACTGAAG CCTATGATCT CCCATACGAA CTTGGAGCCG ATCTCTTCTT TTCGTCCCTA 
CAACTGCAGA GCGTCGCCAC GGGTGGGAAA ATAGGATCGG TACCCCGTCA AGAGCGAAGC 
AAAGATGAGT CCTGGTCCGC GCCAGCTGGC GATCCCCTAC TGAAAGCAGT GAGGGAGAAC 
GATGAAACTG TCTTTAAGCC GCTTCACTTT GACCATGAGT ACCCAGAAGC GAGTGATGAG 
GAGGATGATG AGGATGAACA CGAGGAATTT GATGCGGAAG AAGATGAGGA AGAGTACGAT 
GCAAGGCGTC ACTCACCGCC CATTGTGCCC GCCAGTCACA CAGGTGGTAA GTGGAAACCA 
GAACAGCGGC CGCAGCTACG ACATCCGCAT ATAGAACGCC TCTCGCCAAG TTGGGATGAG 
CCCCCCGAAG AAAACTACAC TCACCCTCCA GCGGACCATA CCAAGGGCAA ATGGGTTCCG 
GGTAGCAAGC AGTTGGAGTA CCGCGAAAAT TTGGATCTTA CCAAGCTGGA CCAACCGGGA 
GCTAGTTATT GGTGCAACAT TTGCTGCAGG CGGCTGAAAA GTCGCTTATA TTATAACCAA 
CATTTGAGGA GTGGCTATCA CATCAAAAGA GCTGAAGCAG AGTGCGAATT AGAGCAGGCT 
ACTCTGGGCA GGGAACTTAC CCTAAGCAAG GACTTCTCAA TAAAAGACGA TGCGGACAAA 
AATGAACCAA AGCCACCGAA ACGACAGAGG AGAGCAAATC TTCTGCGATG CGATCTATGC 
CGGCACACAA TGGCAAGGCA TTTGATTGGC AAACATTTAA TTTCCCATTA TCATTTCAGA 
AGACTCCAAC AGCAGAGTCG AATTAGAAGA CAAGCTTGCT TGCAGGAAAT ACTTAAGCAC 
ATGGGAAGCA TAGTGAGGCA ATCTCCATTT CAATGCATGC CTTGTCGATT CTATGCCAAC 
ACTGAGGAGA CCTTTCTTTA TCATTGGAAA TCCCACGAGC ACTTGGAGTT GACCAAGAGA 
CTGGGGGGGA CTTTTTGGTG TTCCTTTTGC CAGTTTAACT CGAACACCAA TAATTCCATG 
TTGCTGCATT TGTTGGATTC CTCCCATAAG GAGGTGCTTT TAGCCCTTAA TAGATCAGTT 
CCCATATGTA TCGCCCAAAG AAGACGGATC CAGTGCTCTA GGTGTGGAAT GGAATTTTTG 
TACAACATCC AACTACGTCA ACACTCGATC ACTGAGCATC CAGGACTTGC TCTTACAGGG 
ACTGCAGCCG ATGAATACCA GAGTAGGTTT CGATGCAATT TGTGTGGATC CTCACAGAAA 
TCTCGCTTAG CTCTTCAACG CCATAAAAAG CACAAACACC ACTTAGCAAG GTATTTTTGT 
GCCATTTGCC GTTTGGAATT CGACAACTCT TTGGATGCGC GTCGTCATAG AAGCTTGGTG 
ATACATAAGC AAAAAGCCCG ACCCCAAACG TCTATTCCAC AACCCGAAAA CGAAATAGAA 
CATATGCTAA GAGAAGTCCT AGAGGAGACA GTTCCTAGTC CACCCGCCAA AAGAAGTGCG 
GATGTTAACG CAAAGTGCTC GAACAGCAGA AAAACGATTG AATCGTCCCA AAGATTAGCC 
CAACATCAAG CAGAAGTTCA TTCCTCGGAT AATCATTTGT GTTTGAGCTG TGGAATAAGC 
TTTGAGTCAG CTCAGGCACT CGGACGACAC ACCCGCAGCT GTCAACCCCT GGCCAGCACA 
TCAACCCCCG CTGATCTATC ACAAGCTCAA AAGAAATCAA TGTACTCCTG CGATCAATGT 
CGCTTCCAAT CTCAATATGA GTCCGATCTA GTTTATCACC GCATTTTTCA CACACGATCC 
GGAACAATAG GGAAAAACGA GGTTCTTCAG TGCCCTTTGT GCCCGAAGAA TTTCAAAAAG 
CACTCGCTTA GGGCTCACCT AAGGAACCAT ACAAATGAAA AAATTTTTGA ATGCACTGAG 
TGCCTTCAGA AATTTGCTAG AAGACACAAC CTTAAGAACC ACGTAATCAC AAAGCATGCC 
AAAGTAGGCG ACAAGGAGAA GAAAAAATAT GCTGCGAAAG ATGTTGAACA ATCAAAACCC 
AAATATCAGT GTGGGACATG TGGAAAATTA CTGGCCAAAA AGTATTCCCT TAAACTTCAT 
GAGATTAGCC ACTCGAAGGC AGTTGAGCGC AATTATCTCT GCCATTTTGC GGACTGCTCA 
TATGCAGGGC GAACCCCGGA AAGCCTGAAA ACCCACTTGG TCTCCCACTC CCAGGAAAAT 
CACAAATGTG CGCGGATTAA CTGCAGCTAT GTGGGAAAGA GCGAGCTGCA CCTAAAAAGG 
CACCTCAAGT CGGCTCATTC CACGGAGAAG AATGGTGAGG AGTGGTTTTC GTGCGATCAA 
TGCGATTTCA GAGCCCGCAT CAAGGGTCAC CTCCGTCGGC ACTCGTTGCG CCACTCGGGC 
CAGAAGCCCC ACCAATGCCC CCACTGCGAC TTCCAGTGCA GCACCATAGA TAACCTACGT 
AAGCACATCA TCAAGACGGG AAAGCATCCC GGCATGTTCA TTTACCACTG CGCTAAGTGC 
TCCGATGAAG AAGCCGGTGA AATATTTAAG AGCAACAGCT ACAAAGAATA CCAGCACCAC 
TTGAAGACTC ACAAGGCTGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG31612 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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