CG2225 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG2225
Entrez Gene ID	35421
Full Name	CG2225 gene product from transcript CG2225-RH
Synonyms	Dmel\CG2225
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 39E6-39E7

mRNA and Protein(s)

mRNA	Protein	Name
NM_001014497.2	NP_001014497.1	uncharacterized protein, isoform F
NM_001273748.1	NP_001260677.1	uncharacterized protein, isoform H
NM_206020.3	NP_995742.2	uncharacterized protein, isoform E
NM_165393.3	NP_724356.2	uncharacterized protein, isoform B
NM_165392.3	NP_724355.2	uncharacterized protein, isoform A
NM_136274.3	NP_610118.2	uncharacterized protein, isoform C
NM_001259198.2	NP_001246127.1	uncharacterized protein, isoform G

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009860	XP_318975.3
Drosophila melanogaster (fruit fly)	CG2225	NP_995742.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG2225 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG2225 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15141	NM_001014497.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant F (CG2225), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa15142	NM_136274.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG2225), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa15139	NM_165392.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG2225), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa15139	NM_206020.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant E (CG2225), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa15139	NM_001259198.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant G (CG2225), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa15140	NM_001273748.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant H (CG2225), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa15140	NM_165393.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG2225), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG2225 ( NM_001014497.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014497.1 Drosophila melanogaster uncharacterized protein, transcript variant F (CG2225), mRNA.

Price & Availability↑

CloneID	OFa15141
Clone ID Related Accession (Same CDS sequence)	NM_001014497.2
Accession Version	NM_001014497.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001014497.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAAAA AGGATTCCGA AGATGTGCCC CAAACACCCA GCACAGACAC TGTGCCAGAG 
GGAAGCTTCA ACGAAGTGGA TGGTACGGCT GAGAATCTAA CTAAATTATC TGAGGTTGTA 
CACGGACAGG AAACTACATC GCCTCTCATT CAGACAACGC TGCCAAAGGC TAGAGGATCT 
AGCTCAACTC CTGCCGTAAG CTCAAACTAC TCTAGTTTAC CGTGCTGTGG TGTCAGGGCT 
TCGAATTTGG GTGGAGTTTC TGCTGCCAAT TCATCAAGTG GATTGTCAGC CATCCGGCAT 
CCAGGCAGCT GCAACGGGGG TCGCAACGCA CTGGGCATCG ACATTAGCGC TGTTCATAAC 
GCTGTTCTGC GTGGTAATTT GGCGAAGCTG CCGGAACCAT TCGTCAACGA ACATCCAGTG 
ACGATATACC CAGGTCCCTC TTCTAAGACA GCCGTGGGGG AGGGAGCTCG CTGCAAGCGA 
CTACTCAGCT CTGTCGAAAA GCACAAGGAA GTGTGCCACA TGCTAAATCC ACTATCCAGC 
GGCTCAAACT CTTCGTCCGG GGAGGCTTCG CCTGCTGCGT CTCACGTCAA TGTGGCGAAA 
TCAAAAGCTG CTGCCAGGGA TGACGAATAT TCGGAAGATT CGCTGGAGGA GTCAACCATC 
TCTACTGAAC TTACACCCGT TAAACAGAAC GGAGTCGCCT GGGAGATTCA CTTTAGAAAC 
AGCAAGAAGA AATCCAGTGG CTTCAAAAAT ACGTCTGCAT CTAAAACGAA AAGCAACAAT 
TTAGACGACA GTTTCATGTA CAACGAAAGA AGCATGCTGG GCAAAGGGAC TTTTATTATC 
CGTCGCTCCA CAGCCAAAAA ACCGCCTCGT GTCACAGATG TATTTTGTAT ACCAAAGCCA 
CCACGTTCCT CGTTGGCCGA GGGAGTCGGG CCAGAGACCA AAGTTGGAGC CGGGGCGATC 
AGTAATGAGT TCTACACAAG CGATAGCGAG CAAAGCGATG CCACACCCCT ACCACCACCG 
CTGCCACCAG CTTCACCTGA CATAAACTTC GTGTACAAGG AGTGTGAGGC TAATGTGGAG 
CATACACGCC GGGGATTGGA GATGGTCGTC GCACACCGAG CGATCTTATC TCTTGATATC 
GAGGCACCAG AACAACTTGG CTATTGCAAG CCTGTGAAGA GTAAGACGGC GATGCTAACA 
GAAAAGCGCC TGAGTGCCGA GTCCATGCCG GACAACACAT CCAGCAGCGA AGAGTTCGAA 
GAGGCTCGTC TGAACGGAAT CGGCAGCTAC GAGTTGTATG GTTACAACGG TAACGATCAG 
CGAGCTTCCA ATGCCACCAC TCCGCATACA GATTTGGCCC CAACTCTGGA GGAGGACGAA 
GAACTTTCAG ATTTAAGCTA TGGGTGTGCT CCACTGATGC AAATAGAGCA TAACATCAGT 
GCTTTGTTGC GTGGAGACCT TCCAATTATC GGCCAACCGG CTGAGAGGAA AGGAGGTGGG 
CTTACTGGTG CCGCAACGAC AGCTGCCGAG CAGCATGCAA AGCGGGTGCT AGAATTTCGA 
CATGGCGTTC ACAAGTCTGA AAGCGCCAAG GAGATGCTGC TGTCACAGAT GCCCAGTTTG 
GGCCCACTGC CACCATCGCC GCCGAATTCC AACCCGGATT TATTTGACTA CAATGCCCCT 
CTTCCACCGT CACCGGTGGA GCCGCGAAAG CAGCTAGAGC TCCCTGTAGT GCCGAATGCC 
CCTCCCGCGA ATCGGGGCAC TACCGTAGTG GAGGTGCATG CCACCGCCTC TGGTACGGCG 
GTGCACAGTA GCAACAGCCA CACTCGCCGC AGTCGGGACA ATATCGCCTC TGTGCGTCAC 
TTTACCGACG AGCTGCCACC GCCTCCAGCG CCTTCCCACC AATCGGCCAG CTTCTTAGAG 
GGCAACGGCG GACCACCTAC GGTCCCCCCA CATCGAGTGA CTGGAGGTCA TTCGGCTAAT 
ACAATGAAAT CCTGGAGTAT CGACTCGCAA TACCGCAAGA AATCGCCAAA GCTATTTGGT 
CATTATAGCA GCGGTGGCAG TGGGATAACC ACACCCACTG GTCTGGGACC GATTGCTCCA 
CTGCCCCCTC CACATTTCGA CGGAATGGCA GGTGGATCTG GGTCCTACCA TCGTCGTTAT 
ATTAACTATG GCACCAAGCG CAGCCTAAAG CAGTCGCCGC GCGAAGAGCA TCGCCTGCAA 
ACCTCATGTA GTCTTCCTGA AACGCCAATT TTTGCCAGAG GCTGTGACAT ACCACGTACC 
CCATATCGAC GCCAAAATGA GCCCCTGCCT GTGGTCAGTG GCTCCCGCAC AGCGCCCAGA 
TCGAGCACAT CGAACAGCAT CAACATGGGC GCTTCAATTT TGGGGATCGG AGGTGGTAAC 
TATGGTACAG CCCAAATTTG CCGTCAGCGC TCAATAAATC ATGCCTTGGC CTCCAACGAA 
ATGCTTCGCA TGACCGGGGC CCCGGCTCGA GGATGGTATC CCAAGCAACG GGGAATGCGA 
CCCGTCTCCA CCGAGAACAT AGATCGAATG GCTTCCATGC GGGTGTGGGA CAATCCGACG 
GTCATGTCGG GCACCGGACA ATCTCGTAAG CCTTTAACAC TGCCACCAAA TCTTACACCT 
TCCTTCTTGA ATAAATCACC CCGCGAGGCT CTACGGCGTG TTACTAGTTT GCTGATAACC 
AAAAAAAAAA ACAGCAAAGA TCGAAAACAT AAGGCGTACT GCGATCAGCT TATGGATGAT 
AGCAACAGCA AGCATGTCTA TGAATTTGAA TCGGGATCCA CCACGCGAAA GGATACCAAT 
CGCAAAGACA ATGGAGCCTC GGGGGCGACC ACGAATAACA AACCTAAAAA AAAGGGGCTT 
TTTAAATCTC TTTGGAAACG CTCCAAAACG GTCTCTTTAG ATCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014497.1
CDS	124..3051
Translation	MSKKDSEDVPQTPSTDTVPEGSFNEVDGTAENLTKLSEVVHGQETTSPLIQTTLPKARGSSSTPAVSSNYSSLPCCGVRASNLGGVSAANSSSGLSAIRHPGSCNGGRNALGIDISAVHNAVLRGNLAKLPEPFVNEHPVTIYPGPSSKTAVGEGARCKRLLSSVEKHKEVCHMLNPLSSGSNSSSGEASPAASHVNVAKSKAAARDDEYSEDSLEESTISTELTPVKQNGVAWEIHFRNSKKKSSGFKNTSASKTKSNNLDDSFMYNERSMLGKGTFIIRRSTAKKPPRVTDVFCIPKPPRSSLAEGVGPETKVGAGAISNEFYTSDSEQSDATPLPPPLPPASPDINFVYKECEANVEHTRRGLEMVVAHRAILSLDIEAPEQLGYCKPVKSKTAMLTEKRLSAESMPDNTSSSEEFEEARLNGIGSYELYGYNGNDQRASNATTPHTDLAPTLEEDEELSDLSYGCAPLMQIEHNISALLRGDLPIIGQPAERKGGGLTGAATTAAEQHAKRVLEFRHGVHKSESAKEMLLSQMPSLGPLPPSPPNSNPDLFDYNAPLPPSPVEPRKQLELPVVPNAPPANRGTTVVEVHATASGTAVHSSNSHTRRSRDNIASVRHFTDELPPPPAPSHQSASFLEGNGGPPTVPPHRVTGGHSANTMKSWSIDSQYRKKSPKLFGHYSSGGSGITTPTGLGPIAPLPPPHFDGMAGGSGSYHRRYINYGTKRSLKQSPREEHRLQTSCSLPETPIFARGCDIPRTPYRRQNEPLPVVSGSRTAPRSSTSNSINMGASILGIGGGNYGTAQICRQRSINHALASNEMLRMTGAPARGWYPKQRGMRPVSTENIDRMASMRVWDNPTVMSGTGQSRKPLTLPPNLTPSFLNKSPREALRRVTSLLITKKKNSKDRKHKAYCDQLMDDSNSKHVYEFESGSTTRKDTNRKDNGASGATTNNKPKKKGLFKSLWKRSKTVSLDQ

Target ORF information:

RefSeq Version	NM_001014497.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant F (CG2225), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014497.2

ORF Insert Sequence:

ATGTCCAAAA AGGATTCCGA AGATGTGCCC CAAACACCCA GCACAGACAC TGTGCCAGAG 
GGAAGCTTCA ACGAAGTGGA TGGTACGGCT GAGAATCTAA CTAAATTATC TGAGGTTGTA 
CACGGACAGG AAACTACATC GCCTCTCATT CAGACAACGC TGCCAAAGGC TAGAGGATCT 
AGCTCAACTC CTGCCGTAAG CTCAAACTAC TCTAGTTTAC CGTGCTGTGG TGTCAGGGCT 
TCGAATTTGG GTGGAGTTTC TGCTGCCAAT TCATCAAGTG GATTGTCAGC CATCCGGCAT 
CCAGGCAGCT GCAACGGGGG TCGCAACGCA CTGGGCATCG ACATTAGCGC TGTTCATAAC 
GCTGTTCTGC GTGGTAATTT GGCGAAGCTG CCGGAACCAT TCGTCAACGA ACATCCAGTG 
ACGATATACC CAGGTCCCTC TTCTAAGACA GCCGTGGGGG AGGGAGCTCG CTGCAAGCGA 
CTACTCAGCT CTGTCGAAAA GCACAAGGAA GTGTGCCACA TGCTAAATCC ACTATCCAGC 
GGCTCAAACT CTTCGTCCGG GGAGGCTTCG CCTGCTGCGT CTCACGTCAA TGTGGCGAAA 
TCAAAAGCTG CTGCCAGGGA TGACGAATAT TCGGAAGATT CGCTGGAGGA GTCAACCATC 
TCTACTGAAC TTACACCCGT TAAACAGAAC GGAGTCGCCT GGGAGATTCA CTTTAGAAAC 
AGCAAGAAGA AATCCAGTGG CTTCAAAAAT ACGTCTGCAT CTAAAACGAA AAGCAACAAT 
TTAGACGACA GTTTCATGTA CAACGAAAGA AGCATGCTGG GCAAAGGGAC TTTTATTATC 
CGTCGCTCCA CAGCCAAAAA ACCGCCTCGT GTCACAGATG TATTTTGTAT ACCAAAGCCA 
CCACGTTCCT CGTTGGCCGA GGGAGTCGGG CCAGAGACCA AAGTTGGAGC CGGGGCGATC 
AGTAATGAGT TCTACACAAG CGATAGCGAG CAAAGCGATG CCACACCCCT ACCACCACCG 
CTGCCACCAG CTTCACCTGA CATAAACTTC GTGTACAAGG AGTGTGAGGC TAATGTGGAG 
CATACACGCC GGGGATTGGA GATGGTCGTC GCACACCGAG CGATCTTATC TCTTGATATC 
GAGGCACCAG AACAACTTGG CTATTGCAAG CCTGTGAAGA GTAAGACGGC GATGCTAACA 
GAAAAGCGCC TGAGTGCCGA GTCCATGCCG GACAACACAT CCAGCAGCGA AGAGTTCGAA 
GAGGCTCGTC TGAACGGAAT CGGCAGCTAC GAGTTGTATG GTTACAACGG TAACGATCAG 
CGAGCTTCCA ATGCCACCAC TCCGCATACA GATTTGGCCC CAACTCTGGA GGAGGACGAA 
GAACTTTCAG ATTTAAGCTA TGGGTGTGCT CCACTGATGC AAATAGAGCA TAACATCAGT 
GCTTTGTTGC GTGGAGACCT TCCAATTATC GGCCAACCGG CTGAGAGGAA AGGAGGTGGG 
CTTACTGGTG CCGCAACGAC AGCTGCCGAG CAGCATGCAA AGCGGGTGCT AGAATTTCGA 
CATGGCGTTC ACAAGTCTGA AAGCGCCAAG GAGATGCTGC TGTCACAGAT GCCCAGTTTG 
GGCCCACTGC CACCATCGCC GCCGAATTCC AACCCGGATT TATTTGACTA CAATGCCCCT 
CTTCCACCGT CACCGGTGGA GCCGCGAAAG CAGCTAGAGC TCCCTGTAGT GCCGAATGCC 
CCTCCCGCGA ATCGGGGCAC TACCGTAGTG GAGGTGCATG CCACCGCCTC TGGTACGGCG 
GTGCACAGTA GCAACAGCCA CACTCGCCGC AGTCGGGACA ATATCGCCTC TGTGCGTCAC 
TTTACCGACG AGCTGCCACC GCCTCCAGCG CCTTCCCACC AATCGGCCAG CTTCTTAGAG 
GGCAACGGCG GACCACCTAC GGTCCCCCCA CATCGAGTGA CTGGAGGTCA TTCGGCTAAT 
ACAATGAAAT CCTGGAGTAT CGACTCGCAA TACCGCAAGA AATCGCCAAA GCTATTTGGT 
CATTATAGCA GCGGTGGCAG TGGGATAACC ACACCCACTG GTCTGGGACC GATTGCTCCA 
CTGCCCCCTC CACATTTCGA CGGAATGGCA GGTGGATCTG GGTCCTACCA TCGTCGTTAT 
ATTAACTATG GCACCAAGCG CAGCCTAAAG CAGTCGCCGC GCGAAGAGCA TCGCCTGCAA 
ACCTCATGTA GTCTTCCTGA AACGCCAATT TTTGCCAGAG GCTGTGACAT ACCACGTACC 
CCATATCGAC GCCAAAATGA GCCCCTGCCT GTGGTCAGTG GCTCCCGCAC AGCGCCCAGA 
TCGAGCACAT CGAACAGCAT CAACATGGGC GCTTCAATTT TGGGGATCGG AGGTGGTAAC 
TATGGTACAG CCCAAATTTG CCGTCAGCGC TCAATAAATC ATGCCTTGGC CTCCAACGAA 
ATGCTTCGCA TGACCGGGGC CCCGGCTCGA GGATGGTATC CCAAGCAACG GGGAATGCGA 
CCCGTCTCCA CCGAGAACAT AGATCGAATG GCTTCCATGC GGGTGTGGGA CAATCCGACG 
GTCATGTCGG GCACCGGACA ATCTCGTAAG CCTTTAACAC TGCCACCAAA TCTTACACCT 
TCCTTCTTGA ATAAATCACC CCGCGAGGCT CTACGGCGTG TTACTAGTTT GCTGATAACC 
AAAAAAAAAA ACAGCAAAGA TCGAAAACAT AAGGCGTACT GCGATCAGCT TATGGATGAT 
AGCAACAGCA AGCATGTCTA TGAATTTGAA TCGGGATCCA CCACGCGAAA GGATACCAAT 
CGCAAAGACA ATGGAGCCTC GGGGGCGACC ACGAATAACA AACCTAAAAA AAAGGGGCTT 
TTTAAATCTC TTTGGAAACG CTCCAAAACG GTCTCTTTAG ATCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG2225 ( NM_136274.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610118.2 Drosophila melanogaster uncharacterized protein, transcript variant C (CG2225), mRNA.

Price & Availability↑

CloneID	OFa15142
Clone ID Related Accession (Same CDS sequence)	NM_136274.3
Accession Version	NM_136274.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_136274.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACAACG AAAGAAGCAT GCTGGGCAAA GGGACTTTTA TTATCCGTCG CTCCACAGCC 
AAAAAACCGC CTCGTGTCAC AGATGTATTT TGTATACCAA AGCCACCACG TTCCTCGTTG 
GCCGAGGGAG TCGGGCCAGA GACCAAAGTT GGAGCCGGGG CGATCAGTAA TGAGTTCTAC 
ACAAGCGATA GCGAGCAAAG CGATGCCACA CCCCTACCAC CACCGCTGCC ACCAGCTTCA 
CCTGACATAA ACTTCGTGTA CAAGGAGTGT GAGGCTAATG TGGAGCATAC ACGCCGGGGA 
TTGGAGATGG TCGTCGCACA CCGAGCGATC TTATCTCTTG ATATCGAGGC ACCAGAACAA 
CTTGGCTATT GCAAGCCTGT GAAGAGTAAG ACGGCGATGC TAACAGAAAA GCGCCTGAGT 
GCCGAGTCCA TGCCGGACAA CACATCCAGC AGCGAAGAGT TCGAAGAGGC TCGTCTGAAC 
GGAATCGGCA GCTACGAGTT GTATGGTTAC AACGGTAACG ATCAGCGAGC TTCCAATGCC 
ACCACTCCGC ATACAGATTT GGCCCCAACT CTGGAGGAGG ACGAAGAACT TTCAGATTTA 
AGCTATGGGT GTGCTCCACT GATGCAAATA GAGCATAACA TCAGTGCTTT GTTGCGTGGA 
GACCTTCCAA TTATCGGCCA ACCGGCTGAG AGGAAAGGAG GTGGGCTTAC TGGTGCCGCA 
ACGACAGCTG CCGAGCAGCA TGCAAAGCGG GTGCTAGAAT TTCGACATGG CGTTCACAAG 
TCTGAAAGCG CCAAGGAGAT GCTGCTGTCA CAGATGCCCA GTTTGGGCCC ACTGCCACCA 
TCGCCGCCGA ATTCCAACCC GGATTTATTT GACTACAATG CCCCTCTTCC ACCGTCACCG 
GTGGAGCCGC GAAAGCAGCT AGAGCTCCCT GTAGTGCCGA ATGCCCCTCC CGCGAATCGG 
GGCACTACCG TAGTGGAGGT GCATGCCACC GCCTCTGGTA CGGCGGTGCA CAGTAGCAAC 
AGCCACACTC GCCGCAGTCG GGACAATATC GCCTCTGTGC GTCACTTTAC CGACGAGCTG 
CCACCGCCTC CAGCGCCTTC CCACCAATCG GCCAGCTTCT TAGAGGGCAA CGGCGGACCA 
CCTACGGTCC CCCCACATCG AGTGACTGGA GGTCATTCGG CTAATACAAT GAAATCCTGG 
AGTATCGACT CGCAATACCG CAAGAAATCG CCAAAGCTAT TTGGTCATTA TAGCAGCGGT 
GGCAGTGGGA TAACCACACC CACTGGTCTG GGACCGATTG CTCCACTGCC CCCTCCACAT 
TTCGACGGAA TGGCAGGTGG ATCTGGGTCC TACCATCGTC GTTATATTAA CTATGGCACC 
AAGCGCAGCC TAAAGCAGTC GCCGCGCGAA GAGCATCGCC TGCAAACCTC ATGTAGTCTT 
CCTGAAACGC CAATTTTTGC CAGAGGCTGT GACATACCAC GTACCCCATA TCGACGCCAA 
AATGAGCCCC TGCCTGTGGT CAGTGGCTCC CGCACAGCGC CCAGATCGAG CACATCGAAC 
AGCATCAACA TGGGCGCTTC AATTTTGGGG ATCGGAGGTG GTAACTATGG TACAGCCCAA 
ATTTGCCGTC AGCGCTCAAT AAATCATGCC TTGGCCTCCA ACGAAATGCT TCGCATGACC 
GGGGCCCCGG CTCGAGGATG GTATCCCAAG CAACGGGGAA TGCGACCCGT CTCCACCGAG 
AACATAGATC GAATGGCTTC CATGCGGGTG TGGGACAATC CGACGGTCAT GTCGGGCACC 
GGACAATCTC GTAAGCCTTT AACACTGCCA CCAAATCTTA CACCTTCCTT CTTGAATAAA 
TCACCCCGCG AGGCTCTACG GCGTGTTACT AGTTTGCTGA TAACCAAAAA AAAAAACAGC 
AAAGATCGAA AACATAAGGC GTACTGCGAT CAGCTTATGG ATGATAGCAA CAGCAAGCAT 
GTCTATGAAT TTGAATCGGG ATCCACCACG CGAAAGGATA CCAATCGCAA AGACAATGGA 
GCCTCGGGGG CGACCACGAA TAACAAACCT AAAAAAAAGG GGCTTTTTAA ATCTCTTTGG 
AAACGCTCCA AAACGGTCTC TTTAGATCAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610118.2
CDS	124..2256
Translation	MYNERSMLGKGTFIIRRSTAKKPPRVTDVFCIPKPPRSSLAEGVGPETKVGAGAISNEFYTSDSEQSDATPLPPPLPPASPDINFVYKECEANVEHTRRGLEMVVAHRAILSLDIEAPEQLGYCKPVKSKTAMLTEKRLSAESMPDNTSSSEEFEEARLNGIGSYELYGYNGNDQRASNATTPHTDLAPTLEEDEELSDLSYGCAPLMQIEHNISALLRGDLPIIGQPAERKGGGLTGAATTAAEQHAKRVLEFRHGVHKSESAKEMLLSQMPSLGPLPPSPPNSNPDLFDYNAPLPPSPVEPRKQLELPVVPNAPPANRGTTVVEVHATASGTAVHSSNSHTRRSRDNIASVRHFTDELPPPPAPSHQSASFLEGNGGPPTVPPHRVTGGHSANTMKSWSIDSQYRKKSPKLFGHYSSGGSGITTPTGLGPIAPLPPPHFDGMAGGSGSYHRRYINYGTKRSLKQSPREEHRLQTSCSLPETPIFARGCDIPRTPYRRQNEPLPVVSGSRTAPRSSTSNSINMGASILGIGGGNYGTAQICRQRSINHALASNEMLRMTGAPARGWYPKQRGMRPVSTENIDRMASMRVWDNPTVMSGTGQSRKPLTLPPNLTPSFLNKSPREALRRVTSLLITKKKNSKDRKHKAYCDQLMDDSNSKHVYEFESGSTTRKDTNRKDNGASGATTNNKPKKKGLFKSLWKRSKTVSLDQ

Target ORF information:

RefSeq Version	NM_136274.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG2225), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136274.3

ORF Insert Sequence:

ATGTACAACG AAAGAAGCAT GCTGGGCAAA GGGACTTTTA TTATCCGTCG CTCCACAGCC 
AAAAAACCGC CTCGTGTCAC AGATGTATTT TGTATACCAA AGCCACCACG TTCCTCGTTG 
GCCGAGGGAG TCGGGCCAGA GACCAAAGTT GGAGCCGGGG CGATCAGTAA TGAGTTCTAC 
ACAAGCGATA GCGAGCAAAG CGATGCCACA CCCCTACCAC CACCGCTGCC ACCAGCTTCA 
CCTGACATAA ACTTCGTGTA CAAGGAGTGT GAGGCTAATG TGGAGCATAC ACGCCGGGGA 
TTGGAGATGG TCGTCGCACA CCGAGCGATC TTATCTCTTG ATATCGAGGC ACCAGAACAA 
CTTGGCTATT GCAAGCCTGT GAAGAGTAAG ACGGCGATGC TAACAGAAAA GCGCCTGAGT 
GCCGAGTCCA TGCCGGACAA CACATCCAGC AGCGAAGAGT TCGAAGAGGC TCGTCTGAAC 
GGAATCGGCA GCTACGAGTT GTATGGTTAC AACGGTAACG ATCAGCGAGC TTCCAATGCC 
ACCACTCCGC ATACAGATTT GGCCCCAACT CTGGAGGAGG ACGAAGAACT TTCAGATTTA 
AGCTATGGGT GTGCTCCACT GATGCAAATA GAGCATAACA TCAGTGCTTT GTTGCGTGGA 
GACCTTCCAA TTATCGGCCA ACCGGCTGAG AGGAAAGGAG GTGGGCTTAC TGGTGCCGCA 
ACGACAGCTG CCGAGCAGCA TGCAAAGCGG GTGCTAGAAT TTCGACATGG CGTTCACAAG 
TCTGAAAGCG CCAAGGAGAT GCTGCTGTCA CAGATGCCCA GTTTGGGCCC ACTGCCACCA 
TCGCCGCCGA ATTCCAACCC GGATTTATTT GACTACAATG CCCCTCTTCC ACCGTCACCG 
GTGGAGCCGC GAAAGCAGCT AGAGCTCCCT GTAGTGCCGA ATGCCCCTCC CGCGAATCGG 
GGCACTACCG TAGTGGAGGT GCATGCCACC GCCTCTGGTA CGGCGGTGCA CAGTAGCAAC 
AGCCACACTC GCCGCAGTCG GGACAATATC GCCTCTGTGC GTCACTTTAC CGACGAGCTG 
CCACCGCCTC CAGCGCCTTC CCACCAATCG GCCAGCTTCT TAGAGGGCAA CGGCGGACCA 
CCTACGGTCC CCCCACATCG AGTGACTGGA GGTCATTCGG CTAATACAAT GAAATCCTGG 
AGTATCGACT CGCAATACCG CAAGAAATCG CCAAAGCTAT TTGGTCATTA TAGCAGCGGT 
GGCAGTGGGA TAACCACACC CACTGGTCTG GGACCGATTG CTCCACTGCC CCCTCCACAT 
TTCGACGGAA TGGCAGGTGG ATCTGGGTCC TACCATCGTC GTTATATTAA CTATGGCACC 
AAGCGCAGCC TAAAGCAGTC GCCGCGCGAA GAGCATCGCC TGCAAACCTC ATGTAGTCTT 
CCTGAAACGC CAATTTTTGC CAGAGGCTGT GACATACCAC GTACCCCATA TCGACGCCAA 
AATGAGCCCC TGCCTGTGGT CAGTGGCTCC CGCACAGCGC CCAGATCGAG CACATCGAAC 
AGCATCAACA TGGGCGCTTC AATTTTGGGG ATCGGAGGTG GTAACTATGG TACAGCCCAA 
ATTTGCCGTC AGCGCTCAAT AAATCATGCC TTGGCCTCCA ACGAAATGCT TCGCATGACC 
GGGGCCCCGG CTCGAGGATG GTATCCCAAG CAACGGGGAA TGCGACCCGT CTCCACCGAG 
AACATAGATC GAATGGCTTC CATGCGGGTG TGGGACAATC CGACGGTCAT GTCGGGCACC 
GGACAATCTC GTAAGCCTTT AACACTGCCA CCAAATCTTA CACCTTCCTT CTTGAATAAA 
TCACCCCGCG AGGCTCTACG GCGTGTTACT AGTTTGCTGA TAACCAAAAA AAAAAACAGC 
AAAGATCGAA AACATAAGGC GTACTGCGAT CAGCTTATGG ATGATAGCAA CAGCAAGCAT 
GTCTATGAAT TTGAATCGGG ATCCACCACG CGAAAGGATA CCAATCGCAA AGACAATGGA 
GCCTCGGGGG CGACCACGAA TAACAAACCT AAAAAAAAGG GGCTTTTTAA ATCTCTTTGG 
AAACGCTCCA AAACGGTCTC TTTAGATCAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG2225 ( NM_206020.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995742.2 Drosophila melanogaster uncharacterized protein, transcript variant E (CG2225), mRNA.

Price & Availability↑

CloneID	OFa15139
Clone ID Related Accession (Same CDS sequence)	NM_206020.3 , NM_165392.3 , NM_001259198.2
Accession Version	NM_206020.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_206020.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGCGG CACATGAATC GCCTAAAGCA CGCGATTCGA CCAACGTCGA GACTGAGCGG 
ACGTTTCAGC TGGAGGCAGG GCAATCCTAT GACACAATGG CAGAATCTCA TCGCGCATTC 
CATTCCCTGG AATTGGAGGC GGGAGCGGAG GTAGCCGTAG ATGCCGCTGT TGAAGCTGAC 
TCAAAAAGAC AAAACGATAA TCAACTGCAG TCGTTGGAAC CCATTTCCTT GGATGACATA 
CCCGAACCAA TAAAAGTCCT CGACGAGATT ATTTCCGAAT TTGAGGAGGC AGCAACGAAA 
CCAGTGGCCT TGAACTGCAA TAGTGGCGAG AATCAGTCCG AGGACGATGG CTACATGAGC 
CTAAGTCGCA AGAACATGTC CAAAAAGGAT TCCGAAGATG TGCCCCAAAC ACCCAGCACA 
GACACTGTGC CAGAGGGAAG CTTCAACGAA GTGGATGGTA CGGCTGAGAA TCTAACTAAA 
TTATCTGAGG TTGTACACGG ACAGGAAACT ACATCGCCTC TCATTCAGAC AACGCTGCCA 
AAGGCTAGAG GATCTAGCTC AACTCCTGCC GTAAGCTCAA ACTACTCTAG TTTACCGTGC 
TGTGGTGTCA GGGCTTCGAA TTTGGGTGGA GTTTCTGCTG CCAATTCATC AAGTGGATTG 
TCAGCCATCC GGCATCCAGG CAGCTGCAAC GGGGGTCGCA ACGCACTGGG CATCGACATT 
AGCGCTGTTC ATAACGCTGT TCTGCGTGGT AATTTGGCGA AGCTGCCGGA ACCATTCGTC 
AACGAACATC CAGTGACGAT ATACCCAGGT CCCTCTTCTA AGACAGCCGT GGGGGAGGGA 
GCTCGCTGCA AGCGACTACT CAGCTCTGTC GAAAAGCACA AGGAAGTGTG CCACATGCTA 
AATCCACTAT CCAGCGGCTC AAACTCTTCG TCCGGGGAGG CTTCGCCTGC TGCGTCTCAC 
GTCAATGTGG CGAAATCAAA AGCTGCTGCC AGGGATGACG AATATTCGGA AGATTCGCTG 
GAGGAGTCAA CCATCTCTAC TGAACTTACA CCCGTTAAAC AGAACGGAGT CGCCTGGGAG 
ATTCACTTTA GAAACAGCAA GAAGAAATCC AGTGGCTTCA AAAATACGTC TGCATCTAAA 
ACGGTGCGAT CGTTAATAGT TTTTAACATC GTTAAGAAAA GCAACAATTT AGACGACAGT 
TTCATGTACA ACGAAAGAAG CATGCTGGGC AAAGGGACTT TTATTATCCG TCGCTCCACA 
GCCAAAAAAC CGCCTCGTGT CACAGATGTA TTTTGTATAC CAAAGCCACC ACGTTCCTCG 
TTGGCCGAGG GAGTCGGGCC AGAGACCAAA GTTGGAGCCG GGGCGATCAG TAATGAGTTC 
TACACAAGCG ATAGCGAGCA AAGCGATGCC ACACCCCTAC CACCACCGCT GCCACCAGCT 
TCACCTGACA TAAACTTCGT GTACAAGGAG TGTGAGGCTA ATGTGGAGCA TACACGCCGG 
GGATTGGAGA TGGTCGTCGC ACACCGAGCG ATCTTATCTC TTGATATCGA GGCACCAGAA 
CAACTTGGCT ATTGCAAGCC TGTGAAGAGT AAGACGGCGA TGCTAACAGA AAAGCGCCTG 
AGTGCCGAGT CCATGCCGGA CAACACATCC AGCAGCGAAG AGTTCGAAGA GGCTCGTCTG 
AACGGAATCG GCAGCTACGA GTTGTATGGT TACAACGGTA ACGATCAGCG AGCTTCCAAT 
GCCACCACTC CGCATACAGA TTTGGCCCCA ACTCTGGAGG AGGACGAAGA ACTTTCAGAT 
TTAAGCTATG GGTGTGCTCC ACTGATGCAA ATAGAGCATA ACATCAGTGC TTTGTTGCGT 
GGAGACCTTC CAATTATCGG CCAACCGGCT GAGAGGAAAG GAGGTGGGCT TACTGGTGCC 
GCAACGACAG CTGCCGAGCA GCATGCAAAG CGGGTGCTAG AATTTCGACA TGGCGTTCAC 
AAGTCTGAAA GCGCCAAGGA GATGCTGCTG TCACAGATGC CCAGTTTGGG CCCACTGCCA 
CCATCGCCGC CGAATTCCAA CCCGGATTTA TTTGACTACA ATGCCCCTCT TCCACCGTCA 
CCGGTGGAGC CGCGAAAGCA GCTAGAGCTC CCTGTAGTGC CGAATGCCCC TCCCGCGAAT 
CGGGGCACTA CCGTAGTGGA GGTGCATGCC ACCGCCTCTG GTACGGCGGT GCACAGTAGC 
AACAGCCACA CTCGCCGCAG TCGGGACAAT ATCGCCTCTG TGCGTCACTT TACCGACGAG 
CTGCCACCGC CTCCAGCGCC TTCCCACCAA TCGGCCAGCT TCTTAGAGGG CAACGGCGGA 
CCACCTACGG TCCCCCCACA TCGAGTGACT GGAGGTCATT CGGCTAATAC AATGAAATCC 
TGGAGTATCG ACTCGCAATA CCGCAAGAAA TCGCCAAAGC TATTTGGTCA TTATAGCAGC 
GGTGGCAGTG GGATAACCAC ACCCACTGGT CTGGGACCGA TTGCTCCACT GCCCCCTCCA 
CATTTCGACG GAATGGCAGG TGGATCTGGG TCCTACCATC GTCGTTATAT TAACTATGGC 
ACCAAGCGCA GCCTAAAGCA GTCGCCGCGC GAAGAGCATC GCCTGCAAAC CTCATGTAGT 
CTTCCTGAAA CGCCAATTTT TGCCAGAGGC TGTGACATAC CACGTACCCC ATATCGACGC 
CAAAATGAGC CCCTGCCTGT GGTCAGTGGC TCCCGCACAG CGCCCAGATC GAGCACATCG 
AACAGCATCA ACATGGGCGC TTCAATTTTG GGGATCGGAG GTGGTAACTA TGGTACAGCC 
CAAATTTGCC GTCAGCGCTC AATAAATCAT GCCTTGGCCT CCAACGAAAT GCTTCGCATG 
ACCGGGGCCC CGGCTCGAGG ATGGTATCCC AAGCAACGGG GAATGCGACC CGTCTCCACC 
GAGAACATAG ATCGAATGGC TTCCATGCGG GTGTGGGACA ATCCGACGGT CATGTCGGGC 
ACCGGACAAT CTCGTAAGCC TTTAACACTG CCACCAAATC TTACACCTTC CTTCTTGAAT 
AAATCACCCC GCGAGGCTCT ACGGCGTGTT ACTAGTTTGC TGATAACCAA AAAAAAAAAC 
AGCAAAGATC GAAAACATAA GGCGTACTGC GATCAGCTTA TGGATGATAG CAACAGCAAG 
CATGTCTATG AATTTGAATC GGGATCCACC ACGCGAAAGG ATACCAATCG CAAAGACAAT 
GGAGCCTCGG GGGCGACCAC GAATAACAAA CCTAAAAAAA AGGGGCTTTT TAAATCTCTT 
TGGAAACGCT CCAAAACGGT CTCTTTAGAT CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995742.2
CDS	255..3590
Translation	MAAAHESPKARDSTNVETERTFQLEAGQSYDTMAESHRAFHSLELEAGAEVAVDAAVEADSKRQNDNQLQSLEPISLDDIPEPIKVLDEIISEFEEAATKPVALNCNSGENQSEDDGYMSLSRKNMSKKDSEDVPQTPSTDTVPEGSFNEVDGTAENLTKLSEVVHGQETTSPLIQTTLPKARGSSSTPAVSSNYSSLPCCGVRASNLGGVSAANSSSGLSAIRHPGSCNGGRNALGIDISAVHNAVLRGNLAKLPEPFVNEHPVTIYPGPSSKTAVGEGARCKRLLSSVEKHKEVCHMLNPLSSGSNSSSGEASPAASHVNVAKSKAAARDDEYSEDSLEESTISTELTPVKQNGVAWEIHFRNSKKKSSGFKNTSASKTVRSLIVFNIVKKSNNLDDSFMYNERSMLGKGTFIIRRSTAKKPPRVTDVFCIPKPPRSSLAEGVGPETKVGAGAISNEFYTSDSEQSDATPLPPPLPPASPDINFVYKECEANVEHTRRGLEMVVAHRAILSLDIEAPEQLGYCKPVKSKTAMLTEKRLSAESMPDNTSSSEEFEEARLNGIGSYELYGYNGNDQRASNATTPHTDLAPTLEEDEELSDLSYGCAPLMQIEHNISALLRGDLPIIGQPAERKGGGLTGAATTAAEQHAKRVLEFRHGVHKSESAKEMLLSQMPSLGPLPPSPPNSNPDLFDYNAPLPPSPVEPRKQLELPVVPNAPPANRGTTVVEVHATASGTAVHSSNSHTRRSRDNIASVRHFTDELPPPPAPSHQSASFLEGNGGPPTVPPHRVTGGHSANTMKSWSIDSQYRKKSPKLFGHYSSGGSGITTPTGLGPIAPLPPPHFDGMAGGSGSYHRRYINYGTKRSLKQSPREEHRLQTSCSLPETPIFARGCDIPRTPYRRQNEPLPVVSGSRTAPRSSTSNSINMGASILGIGGGNYGTAQICRQRSINHALASNEMLRMTGAPARGWYPKQRGMRPVSTENIDRMASMRVWDNPTVMSGTGQSRKPLTLPPNLTPSFLNKSPREALRRVTSLLITKKKNSKDRKHKAYCDQLMDDSNSKHVYEFESGSTTRKDTNRKDNGASGATTNNKPKKKGLFKSLWKRSKTVSLDQ

Target ORF information:

RefSeq Version	NM_206020.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant E (CG2225), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206020.3

ORF Insert Sequence:

ATGGCAGCGG CACATGAATC GCCTAAAGCA CGCGATTCGA CCAACGTCGA GACTGAGCGG 
ACGTTTCAGC TGGAGGCAGG GCAATCCTAT GACACAATGG CAGAATCTCA TCGCGCATTC 
CATTCCCTGG AATTGGAGGC GGGAGCGGAG GTAGCCGTAG ATGCCGCTGT TGAAGCTGAC 
TCAAAAAGAC AAAACGATAA TCAACTGCAG TCGTTGGAAC CCATTTCCTT GGATGACATA 
CCCGAACCAA TAAAAGTCCT CGACGAGATT ATTTCCGAAT TTGAGGAGGC AGCAACGAAA 
CCAGTGGCCT TGAACTGCAA TAGTGGCGAG AATCAGTCCG AGGACGATGG CTACATGAGC 
CTAAGTCGCA AGAACATGTC CAAAAAGGAT TCCGAAGATG TGCCCCAAAC ACCCAGCACA 
GACACTGTGC CAGAGGGAAG CTTCAACGAA GTGGATGGTA CGGCTGAGAA TCTAACTAAA 
TTATCTGAGG TTGTACACGG ACAGGAAACT ACATCGCCTC TCATTCAGAC AACGCTGCCA 
AAGGCTAGAG GATCTAGCTC AACTCCTGCC GTAAGCTCAA ACTACTCTAG TTTACCGTGC 
TGTGGTGTCA GGGCTTCGAA TTTGGGTGGA GTTTCTGCTG CCAATTCATC AAGTGGATTG 
TCAGCCATCC GGCATCCAGG CAGCTGCAAC GGGGGTCGCA ACGCACTGGG CATCGACATT 
AGCGCTGTTC ATAACGCTGT TCTGCGTGGT AATTTGGCGA AGCTGCCGGA ACCATTCGTC 
AACGAACATC CAGTGACGAT ATACCCAGGT CCCTCTTCTA AGACAGCCGT GGGGGAGGGA 
GCTCGCTGCA AGCGACTACT CAGCTCTGTC GAAAAGCACA AGGAAGTGTG CCACATGCTA 
AATCCACTAT CCAGCGGCTC AAACTCTTCG TCCGGGGAGG CTTCGCCTGC TGCGTCTCAC 
GTCAATGTGG CGAAATCAAA AGCTGCTGCC AGGGATGACG AATATTCGGA AGATTCGCTG 
GAGGAGTCAA CCATCTCTAC TGAACTTACA CCCGTTAAAC AGAACGGAGT CGCCTGGGAG 
ATTCACTTTA GAAACAGCAA GAAGAAATCC AGTGGCTTCA AAAATACGTC TGCATCTAAA 
ACGGTGCGAT CGTTAATAGT TTTTAACATC GTTAAGAAAA GCAACAATTT AGACGACAGT 
TTCATGTACA ACGAAAGAAG CATGCTGGGC AAAGGGACTT TTATTATCCG TCGCTCCACA 
GCCAAAAAAC CGCCTCGTGT CACAGATGTA TTTTGTATAC CAAAGCCACC ACGTTCCTCG 
TTGGCCGAGG GAGTCGGGCC AGAGACCAAA GTTGGAGCCG GGGCGATCAG TAATGAGTTC 
TACACAAGCG ATAGCGAGCA AAGCGATGCC ACACCCCTAC CACCACCGCT GCCACCAGCT 
TCACCTGACA TAAACTTCGT GTACAAGGAG TGTGAGGCTA ATGTGGAGCA TACACGCCGG 
GGATTGGAGA TGGTCGTCGC ACACCGAGCG ATCTTATCTC TTGATATCGA GGCACCAGAA 
CAACTTGGCT ATTGCAAGCC TGTGAAGAGT AAGACGGCGA TGCTAACAGA AAAGCGCCTG 
AGTGCCGAGT CCATGCCGGA CAACACATCC AGCAGCGAAG AGTTCGAAGA GGCTCGTCTG 
AACGGAATCG GCAGCTACGA GTTGTATGGT TACAACGGTA ACGATCAGCG AGCTTCCAAT 
GCCACCACTC CGCATACAGA TTTGGCCCCA ACTCTGGAGG AGGACGAAGA ACTTTCAGAT 
TTAAGCTATG GGTGTGCTCC ACTGATGCAA ATAGAGCATA ACATCAGTGC TTTGTTGCGT 
GGAGACCTTC CAATTATCGG CCAACCGGCT GAGAGGAAAG GAGGTGGGCT TACTGGTGCC 
GCAACGACAG CTGCCGAGCA GCATGCAAAG CGGGTGCTAG AATTTCGACA TGGCGTTCAC 
AAGTCTGAAA GCGCCAAGGA GATGCTGCTG TCACAGATGC CCAGTTTGGG CCCACTGCCA 
CCATCGCCGC CGAATTCCAA CCCGGATTTA TTTGACTACA ATGCCCCTCT TCCACCGTCA 
CCGGTGGAGC CGCGAAAGCA GCTAGAGCTC CCTGTAGTGC CGAATGCCCC TCCCGCGAAT 
CGGGGCACTA CCGTAGTGGA GGTGCATGCC ACCGCCTCTG GTACGGCGGT GCACAGTAGC 
AACAGCCACA CTCGCCGCAG TCGGGACAAT ATCGCCTCTG TGCGTCACTT TACCGACGAG 
CTGCCACCGC CTCCAGCGCC TTCCCACCAA TCGGCCAGCT TCTTAGAGGG CAACGGCGGA 
CCACCTACGG TCCCCCCACA TCGAGTGACT GGAGGTCATT CGGCTAATAC AATGAAATCC 
TGGAGTATCG ACTCGCAATA CCGCAAGAAA TCGCCAAAGC TATTTGGTCA TTATAGCAGC 
GGTGGCAGTG GGATAACCAC ACCCACTGGT CTGGGACCGA TTGCTCCACT GCCCCCTCCA 
CATTTCGACG GAATGGCAGG TGGATCTGGG TCCTACCATC GTCGTTATAT TAACTATGGC 
ACCAAGCGCA GCCTAAAGCA GTCGCCGCGC GAAGAGCATC GCCTGCAAAC CTCATGTAGT 
CTTCCTGAAA CGCCAATTTT TGCCAGAGGC TGTGACATAC CACGTACCCC ATATCGACGC 
CAAAATGAGC CCCTGCCTGT GGTCAGTGGC TCCCGCACAG CGCCCAGATC GAGCACATCG 
AACAGCATCA ACATGGGCGC TTCAATTTTG GGGATCGGAG GTGGTAACTA TGGTACAGCC 
CAAATTTGCC GTCAGCGCTC AATAAATCAT GCCTTGGCCT CCAACGAAAT GCTTCGCATG 
ACCGGGGCCC CGGCTCGAGG ATGGTATCCC AAGCAACGGG GAATGCGACC CGTCTCCACC 
GAGAACATAG ATCGAATGGC TTCCATGCGG GTGTGGGACA ATCCGACGGT CATGTCGGGC 
ACCGGACAAT CTCGTAAGCC TTTAACACTG CCACCAAATC TTACACCTTC CTTCTTGAAT 
AAATCACCCC GCGAGGCTCT ACGGCGTGTT ACTAGTTTGC TGATAACCAA AAAAAAAAAC 
AGCAAAGATC GAAAACATAA GGCGTACTGC GATCAGCTTA TGGATGATAG CAACAGCAAG 
CATGTCTATG AATTTGAATC GGGATCCACC ACGCGAAAGG ATACCAATCG CAAAGACAAT 
GGAGCCTCGG GGGCGACCAC GAATAACAAA CCTAAAAAAA AGGGGCTTTT TAAATCTCTT 
TGGAAACGCT CCAAAACGGT CTCTTTAGAT CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG2225 ( NM_165392.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724355.2 Drosophila melanogaster uncharacterized protein, transcript variant A (CG2225), mRNA.

Price & Availability↑

CloneID	OFa15139
Clone ID Related Accession (Same CDS sequence)	NM_206020.3 , NM_165392.3 , NM_001259198.2
Accession Version	NM_165392.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165392.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGCGG CACATGAATC GCCTAAAGCA CGCGATTCGA CCAACGTCGA GACTGAGCGG 
ACGTTTCAGC TGGAGGCAGG GCAATCCTAT GACACAATGG CAGAATCTCA TCGCGCATTC 
CATTCCCTGG AATTGGAGGC GGGAGCGGAG GTAGCCGTAG ATGCCGCTGT TGAAGCTGAC 
TCAAAAAGAC AAAACGATAA TCAACTGCAG TCGTTGGAAC CCATTTCCTT GGATGACATA 
CCCGAACCAA TAAAAGTCCT CGACGAGATT ATTTCCGAAT TTGAGGAGGC AGCAACGAAA 
CCAGTGGCCT TGAACTGCAA TAGTGGCGAG AATCAGTCCG AGGACGATGG CTACATGAGC 
CTAAGTCGCA AGAACATGTC CAAAAAGGAT TCCGAAGATG TGCCCCAAAC ACCCAGCACA 
GACACTGTGC CAGAGGGAAG CTTCAACGAA GTGGATGGTA CGGCTGAGAA TCTAACTAAA 
TTATCTGAGG TTGTACACGG ACAGGAAACT ACATCGCCTC TCATTCAGAC AACGCTGCCA 
AAGGCTAGAG GATCTAGCTC AACTCCTGCC GTAAGCTCAA ACTACTCTAG TTTACCGTGC 
TGTGGTGTCA GGGCTTCGAA TTTGGGTGGA GTTTCTGCTG CCAATTCATC AAGTGGATTG 
TCAGCCATCC GGCATCCAGG CAGCTGCAAC GGGGGTCGCA ACGCACTGGG CATCGACATT 
AGCGCTGTTC ATAACGCTGT TCTGCGTGGT AATTTGGCGA AGCTGCCGGA ACCATTCGTC 
AACGAACATC CAGTGACGAT ATACCCAGGT CCCTCTTCTA AGACAGCCGT GGGGGAGGGA 
GCTCGCTGCA AGCGACTACT CAGCTCTGTC GAAAAGCACA AGGAAGTGTG CCACATGCTA 
AATCCACTAT CCAGCGGCTC AAACTCTTCG TCCGGGGAGG CTTCGCCTGC TGCGTCTCAC 
GTCAATGTGG CGAAATCAAA AGCTGCTGCC AGGGATGACG AATATTCGGA AGATTCGCTG 
GAGGAGTCAA CCATCTCTAC TGAACTTACA CCCGTTAAAC AGAACGGAGT CGCCTGGGAG 
ATTCACTTTA GAAACAGCAA GAAGAAATCC AGTGGCTTCA AAAATACGTC TGCATCTAAA 
ACGGTGCGAT CGTTAATAGT TTTTAACATC GTTAAGAAAA GCAACAATTT AGACGACAGT 
TTCATGTACA ACGAAAGAAG CATGCTGGGC AAAGGGACTT TTATTATCCG TCGCTCCACA 
GCCAAAAAAC CGCCTCGTGT CACAGATGTA TTTTGTATAC CAAAGCCACC ACGTTCCTCG 
TTGGCCGAGG GAGTCGGGCC AGAGACCAAA GTTGGAGCCG GGGCGATCAG TAATGAGTTC 
TACACAAGCG ATAGCGAGCA AAGCGATGCC ACACCCCTAC CACCACCGCT GCCACCAGCT 
TCACCTGACA TAAACTTCGT GTACAAGGAG TGTGAGGCTA ATGTGGAGCA TACACGCCGG 
GGATTGGAGA TGGTCGTCGC ACACCGAGCG ATCTTATCTC TTGATATCGA GGCACCAGAA 
CAACTTGGCT ATTGCAAGCC TGTGAAGAGT AAGACGGCGA TGCTAACAGA AAAGCGCCTG 
AGTGCCGAGT CCATGCCGGA CAACACATCC AGCAGCGAAG AGTTCGAAGA GGCTCGTCTG 
AACGGAATCG GCAGCTACGA GTTGTATGGT TACAACGGTA ACGATCAGCG AGCTTCCAAT 
GCCACCACTC CGCATACAGA TTTGGCCCCA ACTCTGGAGG AGGACGAAGA ACTTTCAGAT 
TTAAGCTATG GGTGTGCTCC ACTGATGCAA ATAGAGCATA ACATCAGTGC TTTGTTGCGT 
GGAGACCTTC CAATTATCGG CCAACCGGCT GAGAGGAAAG GAGGTGGGCT TACTGGTGCC 
GCAACGACAG CTGCCGAGCA GCATGCAAAG CGGGTGCTAG AATTTCGACA TGGCGTTCAC 
AAGTCTGAAA GCGCCAAGGA GATGCTGCTG TCACAGATGC CCAGTTTGGG CCCACTGCCA 
CCATCGCCGC CGAATTCCAA CCCGGATTTA TTTGACTACA ATGCCCCTCT TCCACCGTCA 
CCGGTGGAGC CGCGAAAGCA GCTAGAGCTC CCTGTAGTGC CGAATGCCCC TCCCGCGAAT 
CGGGGCACTA CCGTAGTGGA GGTGCATGCC ACCGCCTCTG GTACGGCGGT GCACAGTAGC 
AACAGCCACA CTCGCCGCAG TCGGGACAAT ATCGCCTCTG TGCGTCACTT TACCGACGAG 
CTGCCACCGC CTCCAGCGCC TTCCCACCAA TCGGCCAGCT TCTTAGAGGG CAACGGCGGA 
CCACCTACGG TCCCCCCACA TCGAGTGACT GGAGGTCATT CGGCTAATAC AATGAAATCC 
TGGAGTATCG ACTCGCAATA CCGCAAGAAA TCGCCAAAGC TATTTGGTCA TTATAGCAGC 
GGTGGCAGTG GGATAACCAC ACCCACTGGT CTGGGACCGA TTGCTCCACT GCCCCCTCCA 
CATTTCGACG GAATGGCAGG TGGATCTGGG TCCTACCATC GTCGTTATAT TAACTATGGC 
ACCAAGCGCA GCCTAAAGCA GTCGCCGCGC GAAGAGCATC GCCTGCAAAC CTCATGTAGT 
CTTCCTGAAA CGCCAATTTT TGCCAGAGGC TGTGACATAC CACGTACCCC ATATCGACGC 
CAAAATGAGC CCCTGCCTGT GGTCAGTGGC TCCCGCACAG CGCCCAGATC GAGCACATCG 
AACAGCATCA ACATGGGCGC TTCAATTTTG GGGATCGGAG GTGGTAACTA TGGTACAGCC 
CAAATTTGCC GTCAGCGCTC AATAAATCAT GCCTTGGCCT CCAACGAAAT GCTTCGCATG 
ACCGGGGCCC CGGCTCGAGG ATGGTATCCC AAGCAACGGG GAATGCGACC CGTCTCCACC 
GAGAACATAG ATCGAATGGC TTCCATGCGG GTGTGGGACA ATCCGACGGT CATGTCGGGC 
ACCGGACAAT CTCGTAAGCC TTTAACACTG CCACCAAATC TTACACCTTC CTTCTTGAAT 
AAATCACCCC GCGAGGCTCT ACGGCGTGTT ACTAGTTTGC TGATAACCAA AAAAAAAAAC 
AGCAAAGATC GAAAACATAA GGCGTACTGC GATCAGCTTA TGGATGATAG CAACAGCAAG 
CATGTCTATG AATTTGAATC GGGATCCACC ACGCGAAAGG ATACCAATCG CAAAGACAAT 
GGAGCCTCGG GGGCGACCAC GAATAACAAA CCTAAAAAAA AGGGGCTTTT TAAATCTCTT 
TGGAAACGCT CCAAAACGGT CTCTTTAGAT CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724355.2
CDS	399..3734
Translation	MAAAHESPKARDSTNVETERTFQLEAGQSYDTMAESHRAFHSLELEAGAEVAVDAAVEADSKRQNDNQLQSLEPISLDDIPEPIKVLDEIISEFEEAATKPVALNCNSGENQSEDDGYMSLSRKNMSKKDSEDVPQTPSTDTVPEGSFNEVDGTAENLTKLSEVVHGQETTSPLIQTTLPKARGSSSTPAVSSNYSSLPCCGVRASNLGGVSAANSSSGLSAIRHPGSCNGGRNALGIDISAVHNAVLRGNLAKLPEPFVNEHPVTIYPGPSSKTAVGEGARCKRLLSSVEKHKEVCHMLNPLSSGSNSSSGEASPAASHVNVAKSKAAARDDEYSEDSLEESTISTELTPVKQNGVAWEIHFRNSKKKSSGFKNTSASKTVRSLIVFNIVKKSNNLDDSFMYNERSMLGKGTFIIRRSTAKKPPRVTDVFCIPKPPRSSLAEGVGPETKVGAGAISNEFYTSDSEQSDATPLPPPLPPASPDINFVYKECEANVEHTRRGLEMVVAHRAILSLDIEAPEQLGYCKPVKSKTAMLTEKRLSAESMPDNTSSSEEFEEARLNGIGSYELYGYNGNDQRASNATTPHTDLAPTLEEDEELSDLSYGCAPLMQIEHNISALLRGDLPIIGQPAERKGGGLTGAATTAAEQHAKRVLEFRHGVHKSESAKEMLLSQMPSLGPLPPSPPNSNPDLFDYNAPLPPSPVEPRKQLELPVVPNAPPANRGTTVVEVHATASGTAVHSSNSHTRRSRDNIASVRHFTDELPPPPAPSHQSASFLEGNGGPPTVPPHRVTGGHSANTMKSWSIDSQYRKKSPKLFGHYSSGGSGITTPTGLGPIAPLPPPHFDGMAGGSGSYHRRYINYGTKRSLKQSPREEHRLQTSCSLPETPIFARGCDIPRTPYRRQNEPLPVVSGSRTAPRSSTSNSINMGASILGIGGGNYGTAQICRQRSINHALASNEMLRMTGAPARGWYPKQRGMRPVSTENIDRMASMRVWDNPTVMSGTGQSRKPLTLPPNLTPSFLNKSPREALRRVTSLLITKKKNSKDRKHKAYCDQLMDDSNSKHVYEFESGSTTRKDTNRKDNGASGATTNNKPKKKGLFKSLWKRSKTVSLDQ

Target ORF information:

RefSeq Version	NM_165392.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG2225), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165392.3

ORF Insert Sequence:

ATGGCAGCGG CACATGAATC GCCTAAAGCA CGCGATTCGA CCAACGTCGA GACTGAGCGG 
ACGTTTCAGC TGGAGGCAGG GCAATCCTAT GACACAATGG CAGAATCTCA TCGCGCATTC 
CATTCCCTGG AATTGGAGGC GGGAGCGGAG GTAGCCGTAG ATGCCGCTGT TGAAGCTGAC 
TCAAAAAGAC AAAACGATAA TCAACTGCAG TCGTTGGAAC CCATTTCCTT GGATGACATA 
CCCGAACCAA TAAAAGTCCT CGACGAGATT ATTTCCGAAT TTGAGGAGGC AGCAACGAAA 
CCAGTGGCCT TGAACTGCAA TAGTGGCGAG AATCAGTCCG AGGACGATGG CTACATGAGC 
CTAAGTCGCA AGAACATGTC CAAAAAGGAT TCCGAAGATG TGCCCCAAAC ACCCAGCACA 
GACACTGTGC CAGAGGGAAG CTTCAACGAA GTGGATGGTA CGGCTGAGAA TCTAACTAAA 
TTATCTGAGG TTGTACACGG ACAGGAAACT ACATCGCCTC TCATTCAGAC AACGCTGCCA 
AAGGCTAGAG GATCTAGCTC AACTCCTGCC GTAAGCTCAA ACTACTCTAG TTTACCGTGC 
TGTGGTGTCA GGGCTTCGAA TTTGGGTGGA GTTTCTGCTG CCAATTCATC AAGTGGATTG 
TCAGCCATCC GGCATCCAGG CAGCTGCAAC GGGGGTCGCA ACGCACTGGG CATCGACATT 
AGCGCTGTTC ATAACGCTGT TCTGCGTGGT AATTTGGCGA AGCTGCCGGA ACCATTCGTC 
AACGAACATC CAGTGACGAT ATACCCAGGT CCCTCTTCTA AGACAGCCGT GGGGGAGGGA 
GCTCGCTGCA AGCGACTACT CAGCTCTGTC GAAAAGCACA AGGAAGTGTG CCACATGCTA 
AATCCACTAT CCAGCGGCTC AAACTCTTCG TCCGGGGAGG CTTCGCCTGC TGCGTCTCAC 
GTCAATGTGG CGAAATCAAA AGCTGCTGCC AGGGATGACG AATATTCGGA AGATTCGCTG 
GAGGAGTCAA CCATCTCTAC TGAACTTACA CCCGTTAAAC AGAACGGAGT CGCCTGGGAG 
ATTCACTTTA GAAACAGCAA GAAGAAATCC AGTGGCTTCA AAAATACGTC TGCATCTAAA 
ACGGTGCGAT CGTTAATAGT TTTTAACATC GTTAAGAAAA GCAACAATTT AGACGACAGT 
TTCATGTACA ACGAAAGAAG CATGCTGGGC AAAGGGACTT TTATTATCCG TCGCTCCACA 
GCCAAAAAAC CGCCTCGTGT CACAGATGTA TTTTGTATAC CAAAGCCACC ACGTTCCTCG 
TTGGCCGAGG GAGTCGGGCC AGAGACCAAA GTTGGAGCCG GGGCGATCAG TAATGAGTTC 
TACACAAGCG ATAGCGAGCA AAGCGATGCC ACACCCCTAC CACCACCGCT GCCACCAGCT 
TCACCTGACA TAAACTTCGT GTACAAGGAG TGTGAGGCTA ATGTGGAGCA TACACGCCGG 
GGATTGGAGA TGGTCGTCGC ACACCGAGCG ATCTTATCTC TTGATATCGA GGCACCAGAA 
CAACTTGGCT ATTGCAAGCC TGTGAAGAGT AAGACGGCGA TGCTAACAGA AAAGCGCCTG 
AGTGCCGAGT CCATGCCGGA CAACACATCC AGCAGCGAAG AGTTCGAAGA GGCTCGTCTG 
AACGGAATCG GCAGCTACGA GTTGTATGGT TACAACGGTA ACGATCAGCG AGCTTCCAAT 
GCCACCACTC CGCATACAGA TTTGGCCCCA ACTCTGGAGG AGGACGAAGA ACTTTCAGAT 
TTAAGCTATG GGTGTGCTCC ACTGATGCAA ATAGAGCATA ACATCAGTGC TTTGTTGCGT 
GGAGACCTTC CAATTATCGG CCAACCGGCT GAGAGGAAAG GAGGTGGGCT TACTGGTGCC 
GCAACGACAG CTGCCGAGCA GCATGCAAAG CGGGTGCTAG AATTTCGACA TGGCGTTCAC 
AAGTCTGAAA GCGCCAAGGA GATGCTGCTG TCACAGATGC CCAGTTTGGG CCCACTGCCA 
CCATCGCCGC CGAATTCCAA CCCGGATTTA TTTGACTACA ATGCCCCTCT TCCACCGTCA 
CCGGTGGAGC CGCGAAAGCA GCTAGAGCTC CCTGTAGTGC CGAATGCCCC TCCCGCGAAT 
CGGGGCACTA CCGTAGTGGA GGTGCATGCC ACCGCCTCTG GTACGGCGGT GCACAGTAGC 
AACAGCCACA CTCGCCGCAG TCGGGACAAT ATCGCCTCTG TGCGTCACTT TACCGACGAG 
CTGCCACCGC CTCCAGCGCC TTCCCACCAA TCGGCCAGCT TCTTAGAGGG CAACGGCGGA 
CCACCTACGG TCCCCCCACA TCGAGTGACT GGAGGTCATT CGGCTAATAC AATGAAATCC 
TGGAGTATCG ACTCGCAATA CCGCAAGAAA TCGCCAAAGC TATTTGGTCA TTATAGCAGC 
GGTGGCAGTG GGATAACCAC ACCCACTGGT CTGGGACCGA TTGCTCCACT GCCCCCTCCA 
CATTTCGACG GAATGGCAGG TGGATCTGGG TCCTACCATC GTCGTTATAT TAACTATGGC 
ACCAAGCGCA GCCTAAAGCA GTCGCCGCGC GAAGAGCATC GCCTGCAAAC CTCATGTAGT 
CTTCCTGAAA CGCCAATTTT TGCCAGAGGC TGTGACATAC CACGTACCCC ATATCGACGC 
CAAAATGAGC CCCTGCCTGT GGTCAGTGGC TCCCGCACAG CGCCCAGATC GAGCACATCG 
AACAGCATCA ACATGGGCGC TTCAATTTTG GGGATCGGAG GTGGTAACTA TGGTACAGCC 
CAAATTTGCC GTCAGCGCTC AATAAATCAT GCCTTGGCCT CCAACGAAAT GCTTCGCATG 
ACCGGGGCCC CGGCTCGAGG ATGGTATCCC AAGCAACGGG GAATGCGACC CGTCTCCACC 
GAGAACATAG ATCGAATGGC TTCCATGCGG GTGTGGGACA ATCCGACGGT CATGTCGGGC 
ACCGGACAAT CTCGTAAGCC TTTAACACTG CCACCAAATC TTACACCTTC CTTCTTGAAT 
AAATCACCCC GCGAGGCTCT ACGGCGTGTT ACTAGTTTGC TGATAACCAA AAAAAAAAAC 
AGCAAAGATC GAAAACATAA GGCGTACTGC GATCAGCTTA TGGATGATAG CAACAGCAAG 
CATGTCTATG AATTTGAATC GGGATCCACC ACGCGAAAGG ATACCAATCG CAAAGACAAT 
GGAGCCTCGG GGGCGACCAC GAATAACAAA CCTAAAAAAA AGGGGCTTTT TAAATCTCTT 
TGGAAACGCT CCAAAACGGT CTCTTTAGAT CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG2225 ( NM_001259198.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246127.1 Drosophila melanogaster uncharacterized protein, transcript variant G (CG2225), mRNA.

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CloneID	OFa15139
Clone ID Related Accession (Same CDS sequence)	NM_206020.3 , NM_165392.3 , NM_001259198.2
Accession Version	NM_001259198.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001259198.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGCGG CACATGAATC GCCTAAAGCA CGCGATTCGA CCAACGTCGA GACTGAGCGG 
ACGTTTCAGC TGGAGGCAGG GCAATCCTAT GACACAATGG CAGAATCTCA TCGCGCATTC 
CATTCCCTGG AATTGGAGGC GGGAGCGGAG GTAGCCGTAG ATGCCGCTGT TGAAGCTGAC 
TCAAAAAGAC AAAACGATAA TCAACTGCAG TCGTTGGAAC CCATTTCCTT GGATGACATA 
CCCGAACCAA TAAAAGTCCT CGACGAGATT ATTTCCGAAT TTGAGGAGGC AGCAACGAAA 
CCAGTGGCCT TGAACTGCAA TAGTGGCGAG AATCAGTCCG AGGACGATGG CTACATGAGC 
CTAAGTCGCA AGAACATGTC CAAAAAGGAT TCCGAAGATG TGCCCCAAAC ACCCAGCACA 
GACACTGTGC CAGAGGGAAG CTTCAACGAA GTGGATGGTA CGGCTGAGAA TCTAACTAAA 
TTATCTGAGG TTGTACACGG ACAGGAAACT ACATCGCCTC TCATTCAGAC AACGCTGCCA 
AAGGCTAGAG GATCTAGCTC AACTCCTGCC GTAAGCTCAA ACTACTCTAG TTTACCGTGC 
TGTGGTGTCA GGGCTTCGAA TTTGGGTGGA GTTTCTGCTG CCAATTCATC AAGTGGATTG 
TCAGCCATCC GGCATCCAGG CAGCTGCAAC GGGGGTCGCA ACGCACTGGG CATCGACATT 
AGCGCTGTTC ATAACGCTGT TCTGCGTGGT AATTTGGCGA AGCTGCCGGA ACCATTCGTC 
AACGAACATC CAGTGACGAT ATACCCAGGT CCCTCTTCTA AGACAGCCGT GGGGGAGGGA 
GCTCGCTGCA AGCGACTACT CAGCTCTGTC GAAAAGCACA AGGAAGTGTG CCACATGCTA 
AATCCACTAT CCAGCGGCTC AAACTCTTCG TCCGGGGAGG CTTCGCCTGC TGCGTCTCAC 
GTCAATGTGG CGAAATCAAA AGCTGCTGCC AGGGATGACG AATATTCGGA AGATTCGCTG 
GAGGAGTCAA CCATCTCTAC TGAACTTACA CCCGTTAAAC AGAACGGAGT CGCCTGGGAG 
ATTCACTTTA GAAACAGCAA GAAGAAATCC AGTGGCTTCA AAAATACGTC TGCATCTAAA 
ACGGTGCGAT CGTTAATAGT TTTTAACATC GTTAAGAAAA GCAACAATTT AGACGACAGT 
TTCATGTACA ACGAAAGAAG CATGCTGGGC AAAGGGACTT TTATTATCCG TCGCTCCACA 
GCCAAAAAAC CGCCTCGTGT CACAGATGTA TTTTGTATAC CAAAGCCACC ACGTTCCTCG 
TTGGCCGAGG GAGTCGGGCC AGAGACCAAA GTTGGAGCCG GGGCGATCAG TAATGAGTTC 
TACACAAGCG ATAGCGAGCA AAGCGATGCC ACACCCCTAC CACCACCGCT GCCACCAGCT 
TCACCTGACA TAAACTTCGT GTACAAGGAG TGTGAGGCTA ATGTGGAGCA TACACGCCGG 
GGATTGGAGA TGGTCGTCGC ACACCGAGCG ATCTTATCTC TTGATATCGA GGCACCAGAA 
CAACTTGGCT ATTGCAAGCC TGTGAAGAGT AAGACGGCGA TGCTAACAGA AAAGCGCCTG 
AGTGCCGAGT CCATGCCGGA CAACACATCC AGCAGCGAAG AGTTCGAAGA GGCTCGTCTG 
AACGGAATCG GCAGCTACGA GTTGTATGGT TACAACGGTA ACGATCAGCG AGCTTCCAAT 
GCCACCACTC CGCATACAGA TTTGGCCCCA ACTCTGGAGG AGGACGAAGA ACTTTCAGAT 
TTAAGCTATG GGTGTGCTCC ACTGATGCAA ATAGAGCATA ACATCAGTGC TTTGTTGCGT 
GGAGACCTTC CAATTATCGG CCAACCGGCT GAGAGGAAAG GAGGTGGGCT TACTGGTGCC 
GCAACGACAG CTGCCGAGCA GCATGCAAAG CGGGTGCTAG AATTTCGACA TGGCGTTCAC 
AAGTCTGAAA GCGCCAAGGA GATGCTGCTG TCACAGATGC CCAGTTTGGG CCCACTGCCA 
CCATCGCCGC CGAATTCCAA CCCGGATTTA TTTGACTACA ATGCCCCTCT TCCACCGTCA 
CCGGTGGAGC CGCGAAAGCA GCTAGAGCTC CCTGTAGTGC CGAATGCCCC TCCCGCGAAT 
CGGGGCACTA CCGTAGTGGA GGTGCATGCC ACCGCCTCTG GTACGGCGGT GCACAGTAGC 
AACAGCCACA CTCGCCGCAG TCGGGACAAT ATCGCCTCTG TGCGTCACTT TACCGACGAG 
CTGCCACCGC CTCCAGCGCC TTCCCACCAA TCGGCCAGCT TCTTAGAGGG CAACGGCGGA 
CCACCTACGG TCCCCCCACA TCGAGTGACT GGAGGTCATT CGGCTAATAC AATGAAATCC 
TGGAGTATCG ACTCGCAATA CCGCAAGAAA TCGCCAAAGC TATTTGGTCA TTATAGCAGC 
GGTGGCAGTG GGATAACCAC ACCCACTGGT CTGGGACCGA TTGCTCCACT GCCCCCTCCA 
CATTTCGACG GAATGGCAGG TGGATCTGGG TCCTACCATC GTCGTTATAT TAACTATGGC 
ACCAAGCGCA GCCTAAAGCA GTCGCCGCGC GAAGAGCATC GCCTGCAAAC CTCATGTAGT 
CTTCCTGAAA CGCCAATTTT TGCCAGAGGC TGTGACATAC CACGTACCCC ATATCGACGC 
CAAAATGAGC CCCTGCCTGT GGTCAGTGGC TCCCGCACAG CGCCCAGATC GAGCACATCG 
AACAGCATCA ACATGGGCGC TTCAATTTTG GGGATCGGAG GTGGTAACTA TGGTACAGCC 
CAAATTTGCC GTCAGCGCTC AATAAATCAT GCCTTGGCCT CCAACGAAAT GCTTCGCATG 
ACCGGGGCCC CGGCTCGAGG ATGGTATCCC AAGCAACGGG GAATGCGACC CGTCTCCACC 
GAGAACATAG ATCGAATGGC TTCCATGCGG GTGTGGGACA ATCCGACGGT CATGTCGGGC 
ACCGGACAAT CTCGTAAGCC TTTAACACTG CCACCAAATC TTACACCTTC CTTCTTGAAT 
AAATCACCCC GCGAGGCTCT ACGGCGTGTT ACTAGTTTGC TGATAACCAA AAAAAAAAAC 
AGCAAAGATC GAAAACATAA GGCGTACTGC GATCAGCTTA TGGATGATAG CAACAGCAAG 
CATGTCTATG AATTTGAATC GGGATCCACC ACGCGAAAGG ATACCAATCG CAAAGACAAT 
GGAGCCTCGG GGGCGACCAC GAATAACAAA CCTAAAAAAA AGGGGCTTTT TAAATCTCTT 
TGGAAACGCT CCAAAACGGT CTCTTTAGAT CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246127.1
CDS	636..3971
Translation	MAAAHESPKARDSTNVETERTFQLEAGQSYDTMAESHRAFHSLELEAGAEVAVDAAVEADSKRQNDNQLQSLEPISLDDIPEPIKVLDEIISEFEEAATKPVALNCNSGENQSEDDGYMSLSRKNMSKKDSEDVPQTPSTDTVPEGSFNEVDGTAENLTKLSEVVHGQETTSPLIQTTLPKARGSSSTPAVSSNYSSLPCCGVRASNLGGVSAANSSSGLSAIRHPGSCNGGRNALGIDISAVHNAVLRGNLAKLPEPFVNEHPVTIYPGPSSKTAVGEGARCKRLLSSVEKHKEVCHMLNPLSSGSNSSSGEASPAASHVNVAKSKAAARDDEYSEDSLEESTISTELTPVKQNGVAWEIHFRNSKKKSSGFKNTSASKTVRSLIVFNIVKKSNNLDDSFMYNERSMLGKGTFIIRRSTAKKPPRVTDVFCIPKPPRSSLAEGVGPETKVGAGAISNEFYTSDSEQSDATPLPPPLPPASPDINFVYKECEANVEHTRRGLEMVVAHRAILSLDIEAPEQLGYCKPVKSKTAMLTEKRLSAESMPDNTSSSEEFEEARLNGIGSYELYGYNGNDQRASNATTPHTDLAPTLEEDEELSDLSYGCAPLMQIEHNISALLRGDLPIIGQPAERKGGGLTGAATTAAEQHAKRVLEFRHGVHKSESAKEMLLSQMPSLGPLPPSPPNSNPDLFDYNAPLPPSPVEPRKQLELPVVPNAPPANRGTTVVEVHATASGTAVHSSNSHTRRSRDNIASVRHFTDELPPPPAPSHQSASFLEGNGGPPTVPPHRVTGGHSANTMKSWSIDSQYRKKSPKLFGHYSSGGSGITTPTGLGPIAPLPPPHFDGMAGGSGSYHRRYINYGTKRSLKQSPREEHRLQTSCSLPETPIFARGCDIPRTPYRRQNEPLPVVSGSRTAPRSSTSNSINMGASILGIGGGNYGTAQICRQRSINHALASNEMLRMTGAPARGWYPKQRGMRPVSTENIDRMASMRVWDNPTVMSGTGQSRKPLTLPPNLTPSFLNKSPREALRRVTSLLITKKKNSKDRKHKAYCDQLMDDSNSKHVYEFESGSTTRKDTNRKDNGASGATTNNKPKKKGLFKSLWKRSKTVSLDQ

Target ORF information:

RefSeq Version	NM_001259198.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant G (CG2225), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259198.2

ORF Insert Sequence:

ATGGCAGCGG CACATGAATC GCCTAAAGCA CGCGATTCGA CCAACGTCGA GACTGAGCGG 
ACGTTTCAGC TGGAGGCAGG GCAATCCTAT GACACAATGG CAGAATCTCA TCGCGCATTC 
CATTCCCTGG AATTGGAGGC GGGAGCGGAG GTAGCCGTAG ATGCCGCTGT TGAAGCTGAC 
TCAAAAAGAC AAAACGATAA TCAACTGCAG TCGTTGGAAC CCATTTCCTT GGATGACATA 
CCCGAACCAA TAAAAGTCCT CGACGAGATT ATTTCCGAAT TTGAGGAGGC AGCAACGAAA 
CCAGTGGCCT TGAACTGCAA TAGTGGCGAG AATCAGTCCG AGGACGATGG CTACATGAGC 
CTAAGTCGCA AGAACATGTC CAAAAAGGAT TCCGAAGATG TGCCCCAAAC ACCCAGCACA 
GACACTGTGC CAGAGGGAAG CTTCAACGAA GTGGATGGTA CGGCTGAGAA TCTAACTAAA 
TTATCTGAGG TTGTACACGG ACAGGAAACT ACATCGCCTC TCATTCAGAC AACGCTGCCA 
AAGGCTAGAG GATCTAGCTC AACTCCTGCC GTAAGCTCAA ACTACTCTAG TTTACCGTGC 
TGTGGTGTCA GGGCTTCGAA TTTGGGTGGA GTTTCTGCTG CCAATTCATC AAGTGGATTG 
TCAGCCATCC GGCATCCAGG CAGCTGCAAC GGGGGTCGCA ACGCACTGGG CATCGACATT 
AGCGCTGTTC ATAACGCTGT TCTGCGTGGT AATTTGGCGA AGCTGCCGGA ACCATTCGTC 
AACGAACATC CAGTGACGAT ATACCCAGGT CCCTCTTCTA AGACAGCCGT GGGGGAGGGA 
GCTCGCTGCA AGCGACTACT CAGCTCTGTC GAAAAGCACA AGGAAGTGTG CCACATGCTA 
AATCCACTAT CCAGCGGCTC AAACTCTTCG TCCGGGGAGG CTTCGCCTGC TGCGTCTCAC 
GTCAATGTGG CGAAATCAAA AGCTGCTGCC AGGGATGACG AATATTCGGA AGATTCGCTG 
GAGGAGTCAA CCATCTCTAC TGAACTTACA CCCGTTAAAC AGAACGGAGT CGCCTGGGAG 
ATTCACTTTA GAAACAGCAA GAAGAAATCC AGTGGCTTCA AAAATACGTC TGCATCTAAA 
ACGGTGCGAT CGTTAATAGT TTTTAACATC GTTAAGAAAA GCAACAATTT AGACGACAGT 
TTCATGTACA ACGAAAGAAG CATGCTGGGC AAAGGGACTT TTATTATCCG TCGCTCCACA 
GCCAAAAAAC CGCCTCGTGT CACAGATGTA TTTTGTATAC CAAAGCCACC ACGTTCCTCG 
TTGGCCGAGG GAGTCGGGCC AGAGACCAAA GTTGGAGCCG GGGCGATCAG TAATGAGTTC 
TACACAAGCG ATAGCGAGCA AAGCGATGCC ACACCCCTAC CACCACCGCT GCCACCAGCT 
TCACCTGACA TAAACTTCGT GTACAAGGAG TGTGAGGCTA ATGTGGAGCA TACACGCCGG 
GGATTGGAGA TGGTCGTCGC ACACCGAGCG ATCTTATCTC TTGATATCGA GGCACCAGAA 
CAACTTGGCT ATTGCAAGCC TGTGAAGAGT AAGACGGCGA TGCTAACAGA AAAGCGCCTG 
AGTGCCGAGT CCATGCCGGA CAACACATCC AGCAGCGAAG AGTTCGAAGA GGCTCGTCTG 
AACGGAATCG GCAGCTACGA GTTGTATGGT TACAACGGTA ACGATCAGCG AGCTTCCAAT 
GCCACCACTC CGCATACAGA TTTGGCCCCA ACTCTGGAGG AGGACGAAGA ACTTTCAGAT 
TTAAGCTATG GGTGTGCTCC ACTGATGCAA ATAGAGCATA ACATCAGTGC TTTGTTGCGT 
GGAGACCTTC CAATTATCGG CCAACCGGCT GAGAGGAAAG GAGGTGGGCT TACTGGTGCC 
GCAACGACAG CTGCCGAGCA GCATGCAAAG CGGGTGCTAG AATTTCGACA TGGCGTTCAC 
AAGTCTGAAA GCGCCAAGGA GATGCTGCTG TCACAGATGC CCAGTTTGGG CCCACTGCCA 
CCATCGCCGC CGAATTCCAA CCCGGATTTA TTTGACTACA ATGCCCCTCT TCCACCGTCA 
CCGGTGGAGC CGCGAAAGCA GCTAGAGCTC CCTGTAGTGC CGAATGCCCC TCCCGCGAAT 
CGGGGCACTA CCGTAGTGGA GGTGCATGCC ACCGCCTCTG GTACGGCGGT GCACAGTAGC 
AACAGCCACA CTCGCCGCAG TCGGGACAAT ATCGCCTCTG TGCGTCACTT TACCGACGAG 
CTGCCACCGC CTCCAGCGCC TTCCCACCAA TCGGCCAGCT TCTTAGAGGG CAACGGCGGA 
CCACCTACGG TCCCCCCACA TCGAGTGACT GGAGGTCATT CGGCTAATAC AATGAAATCC 
TGGAGTATCG ACTCGCAATA CCGCAAGAAA TCGCCAAAGC TATTTGGTCA TTATAGCAGC 
GGTGGCAGTG GGATAACCAC ACCCACTGGT CTGGGACCGA TTGCTCCACT GCCCCCTCCA 
CATTTCGACG GAATGGCAGG TGGATCTGGG TCCTACCATC GTCGTTATAT TAACTATGGC 
ACCAAGCGCA GCCTAAAGCA GTCGCCGCGC GAAGAGCATC GCCTGCAAAC CTCATGTAGT 
CTTCCTGAAA CGCCAATTTT TGCCAGAGGC TGTGACATAC CACGTACCCC ATATCGACGC 
CAAAATGAGC CCCTGCCTGT GGTCAGTGGC TCCCGCACAG CGCCCAGATC GAGCACATCG 
AACAGCATCA ACATGGGCGC TTCAATTTTG GGGATCGGAG GTGGTAACTA TGGTACAGCC 
CAAATTTGCC GTCAGCGCTC AATAAATCAT GCCTTGGCCT CCAACGAAAT GCTTCGCATG 
ACCGGGGCCC CGGCTCGAGG ATGGTATCCC AAGCAACGGG GAATGCGACC CGTCTCCACC 
GAGAACATAG ATCGAATGGC TTCCATGCGG GTGTGGGACA ATCCGACGGT CATGTCGGGC 
ACCGGACAAT CTCGTAAGCC TTTAACACTG CCACCAAATC TTACACCTTC CTTCTTGAAT 
AAATCACCCC GCGAGGCTCT ACGGCGTGTT ACTAGTTTGC TGATAACCAA AAAAAAAAAC 
AGCAAAGATC GAAAACATAA GGCGTACTGC GATCAGCTTA TGGATGATAG CAACAGCAAG 
CATGTCTATG AATTTGAATC GGGATCCACC ACGCGAAAGG ATACCAATCG CAAAGACAAT 
GGAGCCTCGG GGGCGACCAC GAATAACAAA CCTAAAAAAA AGGGGCTTTT TAAATCTCTT 
TGGAAACGCT CCAAAACGGT CTCTTTAGAT CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG2225 ( NM_001273748.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260677.1 Drosophila melanogaster uncharacterized protein, transcript variant H (CG2225), mRNA.

Price & Availability↑

CloneID	OFa15140
Clone ID Related Accession (Same CDS sequence)	NM_001273748.1 , NM_165393.3
Accession Version	NM_001273748.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGCGG CACATGAATC GCCTAAAGCA CGCGATTCGA CCAACGTCGA GACTGAGCGG 
ACGTTTCAGC TGGAGGCAGG GCAATCCTAT GACACAATGG CAGAATCTCA TCGCGCATTC 
CATTCCCTGG AATTGGAGGC GGGAGCGGAG GTAGCCGTAG ATGCCGCTGT TGAAGCTGAC 
TCAAAAAGAC AAAACGATAA TCAACTGCAG TCGTTGGAAC CCATTTCCTT GGATGACATA 
CCCGAACCAA TAAAAGTCCT CGACGAGATT ATTTCCGAAT TTGAGGAGGC AGCAACGAAA 
CCAGTGGCCT TGAACTGCAA TAGTGGCGAG AATCAGTCCG AGGACGATGG CTACATGAGC 
CTAAGTCGCA AGAACATGTC CAAAAAGGAT TCCGAAGATG TGCCCCAAAC ACCCAGCACA 
GACACTGTGC CAGAGGGAAG CTTCAACGAA GTGGATGGTA CGGCTGAGAA TCTAACTAAA 
TTATCTGAGG TTGTACACGG ACAGGAAACT ACATCGCCTC TCATTCAGAC AACGCTGCCA 
AAGGCTAGAG GATCTAGCTC AACTCCTGCC GTAAGCTCAA ACTACTCTAG TTTACCGTGC 
TGTGGTGTCA GGGCTTCGAA TTTGGGTGGA GTTTCTGCTG CCAATTCATC AAGTGGATTG 
TCAGCCATCC GGCATCCAGG CAGCTGCAAC GGGGGTCGCA ACGCACTGGG CATCGACATT 
AGCGCTGTTC ATAACGCTGT TCTGCGTGGT AATTTGGCGA AGCTGCCGGA ACCATTCGTC 
AACGAACATC CAGTGACGAT ATACCCAGGT CCCTCTTCTA AGACAGCCGT GGGGGAGGGA 
GCTCGCTGCA AGCGACTACT CAGCTCTGTC GAAAAGCACA AGGAAGTGTG CCACATGCTA 
AATCCACTAT CCAGCGGCTC AAACTCTTCG TCCGGGGAGG CTTCGCCTGC TGCGTCTCAC 
GTCAATGTGG CGAAATCAAA AGCTGCTGCC AGGGATGACG AATATTCGGA AGATTCGCTG 
GAGGAGTCAA CCATCTCTAC TGAACTTACA CCCGTTAAAC AGAACGGAGT CGCCTGGGAG 
ATTCACTTTA GAAACAGCAA GAAGAAATCC AGTGGCTTCA AAAATACGTC TGCATCTAAA 
ACGAAAAGCA ACAATTTAGA CGACAGTTTC ATGTACAACG AAAGAAGCAT GCTGGGCAAA 
GGGACTTTTA TTATCCGTCG CTCCACAGCC AAAAAACCGC CTCGTGTCAC AGATGTATTT 
TGTATACCAA AGCCACCACG TTCCTCGTTG GCCGAGGGAG TCGGGCCAGA GACCAAAGTT 
GGAGCCGGGG CGATCAGTAA TGAGTTCTAC ACAAGCGATA GCGAGCAAAG CGATGCCACA 
CCCCTACCAC CACCGCTGCC ACCAGCTTCA CCTGACATAA ACTTCGTGTA CAAGGAGTGT 
GAGGCTAATG TGGAGCATAC ACGCCGGGGA TTGGAGATGG TCGTCGCACA CCGAGCGATC 
TTATCTCTTG ATATCGAGGC ACCAGAACAA CTTGGCTATT GCAAGCCTGT GAAGAGTAAG 
ACGGCGATGC TAACAGAAAA GCGCCTGAGT GCCGAGTCCA TGCCGGACAA CACATCCAGC 
AGCGAAGAGT TCGAAGAGGC TCGTCTGAAC GGAATCGGCA GCTACGAGTT GTATGGTTAC 
AACGGTAACG ATCAGCGAGC TTCCAATGCC ACCACTCCGC ATACAGATTT GGCCCCAACT 
CTGGAGGAGG ACGAAGAACT TTCAGATTTA AGCTATGGGT GTGCTCCACT GATGCAAATA 
GAGCATAACA TCAGTGCTTT GTTGCGTGGA GACCTTCCAA TTATCGGCCA ACCGGCTGAG 
AGGAAAGGAG GTGGGCTTAC TGGTGCCGCA ACGACAGCTG CCGAGCAGCA TGCAAAGCGG 
GTGCTAGAAT TTCGACATGG CGTTCACAAG TCTGAAAGCG CCAAGGAGAT GCTGCTGTCA 
CAGATGCCCA GTTTGGGCCC ACTGCCACCA TCGCCGCCGA ATTCCAACCC GGATTTATTT 
GACTACAATG CCCCTCTTCC ACCGTCACCG GTGGAGCCGC GAAAGCAGCT AGAGCTCCCT 
GTAGTGCCGA ATGCCCCTCC CGCGAATCGG GGCACTACCG TAGTGGAGGT GCATGCCACC 
GCCTCTGGTA CGGCGGTGCA CAGTAGCAAC AGCCACACTC GCCGCAGTCG GGACAATATC 
GCCTCTGTGC GTCACTTTAC CGACGAGCTG CCACCGCCTC CAGCGCCTTC CCACCAATCG 
GCCAGCTTCT TAGAGGGCAA CGGCGGACCA CCTACGGTCC CCCCACATCG AGTGACTGGA 
GGTCATTCGG CTAATACAAT GAAATCCTGG AGTATCGACT CGCAATACCG CAAGAAATCG 
CCAAAGCTAT TTGGTCATTA TAGCAGCGGT GGCAGTGGGA TAACCACACC CACTGGTCTG 
GGACCGATTG CTCCACTGCC CCCTCCACAT TTCGACGGAA TGGCAGGTGG ATCTGGGTCC 
TACCATCGTC GTTATATTAA CTATGGCACC AAGCGCAGCC TAAAGCAGTC GCCGCGCGAA 
GAGCATCGCC TGCAAACCTC ATGTAGTCTT CCTGAAACGC CAATTTTTGC CAGAGGCTGT 
GACATACCAC GTACCCCATA TCGACGCCAA AATGAGCCCC TGCCTGTGGT CAGTGGCTCC 
CGCACAGCGC CCAGATCGAG CACATCGAAC AGCATCAACA TGGGCGCTTC AATTTTGGGG 
ATCGGAGGTG GTAACTATGG TACAGCCCAA ATTTGCCGTC AGCGCTCAAT AAATCATGCC 
TTGGCCTCCA ACGAAATGCT TCGCATGACC GGGGCCCCGG CTCGAGGATG GTATCCCAAG 
CAACGGGGAA TGCGACCCGT CTCCACCGAG AACATAGATC GAATGGCTTC CATGCGGGTG 
TGGGACAATC CGACGGTCAT GTCGGGCACC GGACAATCTC GTAAGCCTTT AACACTGCCA 
CCAAATCTTA CACCTTCCTT CTTGAATAAA TCACCCCGCG AGGCTCTACG GCGTGTTACT 
AGTTTGCTGA TAACCAAAAA AAAAAACAGC AAAGATCGAA AACATAAGGC GTACTGCGAT 
CAGCTTATGG ATGATAGCAA CAGCAAGCAT GTCTATGAAT TTGAATCGGG ATCCACCACG 
CGAAAGGATA CCAATCGCAA AGACAATGGA GCCTCGGGGG CGACCACGAA TAACAAACCT 
AAAAAAAAGG GGCTTTTTAA ATCTCTTTGG AAACGCTCCA AAACGGTCTC TTTAGATCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260677.1
CDS	255..3557
Translation	MAAAHESPKARDSTNVETERTFQLEAGQSYDTMAESHRAFHSLELEAGAEVAVDAAVEADSKRQNDNQLQSLEPISLDDIPEPIKVLDEIISEFEEAATKPVALNCNSGENQSEDDGYMSLSRKNMSKKDSEDVPQTPSTDTVPEGSFNEVDGTAENLTKLSEVVHGQETTSPLIQTTLPKARGSSSTPAVSSNYSSLPCCGVRASNLGGVSAANSSSGLSAIRHPGSCNGGRNALGIDISAVHNAVLRGNLAKLPEPFVNEHPVTIYPGPSSKTAVGEGARCKRLLSSVEKHKEVCHMLNPLSSGSNSSSGEASPAASHVNVAKSKAAARDDEYSEDSLEESTISTELTPVKQNGVAWEIHFRNSKKKSSGFKNTSASKTKSNNLDDSFMYNERSMLGKGTFIIRRSTAKKPPRVTDVFCIPKPPRSSLAEGVGPETKVGAGAISNEFYTSDSEQSDATPLPPPLPPASPDINFVYKECEANVEHTRRGLEMVVAHRAILSLDIEAPEQLGYCKPVKSKTAMLTEKRLSAESMPDNTSSSEEFEEARLNGIGSYELYGYNGNDQRASNATTPHTDLAPTLEEDEELSDLSYGCAPLMQIEHNISALLRGDLPIIGQPAERKGGGLTGAATTAAEQHAKRVLEFRHGVHKSESAKEMLLSQMPSLGPLPPSPPNSNPDLFDYNAPLPPSPVEPRKQLELPVVPNAPPANRGTTVVEVHATASGTAVHSSNSHTRRSRDNIASVRHFTDELPPPPAPSHQSASFLEGNGGPPTVPPHRVTGGHSANTMKSWSIDSQYRKKSPKLFGHYSSGGSGITTPTGLGPIAPLPPPHFDGMAGGSGSYHRRYINYGTKRSLKQSPREEHRLQTSCSLPETPIFARGCDIPRTPYRRQNEPLPVVSGSRTAPRSSTSNSINMGASILGIGGGNYGTAQICRQRSINHALASNEMLRMTGAPARGWYPKQRGMRPVSTENIDRMASMRVWDNPTVMSGTGQSRKPLTLPPNLTPSFLNKSPREALRRVTSLLITKKKNSKDRKHKAYCDQLMDDSNSKHVYEFESGSTTRKDTNRKDNGASGATTNNKPKKKGLFKSLWKRSKTVSLDQ

Target ORF information:

RefSeq Version	NM_001273748.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant H (CG2225), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273748.1

ORF Insert Sequence:

ATGGCAGCGG CACATGAATC GCCTAAAGCA CGCGATTCGA CCAACGTCGA GACTGAGCGG 
ACGTTTCAGC TGGAGGCAGG GCAATCCTAT GACACAATGG CAGAATCTCA TCGCGCATTC 
CATTCCCTGG AATTGGAGGC GGGAGCGGAG GTAGCCGTAG ATGCCGCTGT TGAAGCTGAC 
TCAAAAAGAC AAAACGATAA TCAACTGCAG TCGTTGGAAC CCATTTCCTT GGATGACATA 
CCCGAACCAA TAAAAGTCCT CGACGAGATT ATTTCCGAAT TTGAGGAGGC AGCAACGAAA 
CCAGTGGCCT TGAACTGCAA TAGTGGCGAG AATCAGTCCG AGGACGATGG CTACATGAGC 
CTAAGTCGCA AGAACATGTC CAAAAAGGAT TCCGAAGATG TGCCCCAAAC ACCCAGCACA 
GACACTGTGC CAGAGGGAAG CTTCAACGAA GTGGATGGTA CGGCTGAGAA TCTAACTAAA 
TTATCTGAGG TTGTACACGG ACAGGAAACT ACATCGCCTC TCATTCAGAC AACGCTGCCA 
AAGGCTAGAG GATCTAGCTC AACTCCTGCC GTAAGCTCAA ACTACTCTAG TTTACCGTGC 
TGTGGTGTCA GGGCTTCGAA TTTGGGTGGA GTTTCTGCTG CCAATTCATC AAGTGGATTG 
TCAGCCATCC GGCATCCAGG CAGCTGCAAC GGGGGTCGCA ACGCACTGGG CATCGACATT 
AGCGCTGTTC ATAACGCTGT TCTGCGTGGT AATTTGGCGA AGCTGCCGGA ACCATTCGTC 
AACGAACATC CAGTGACGAT ATACCCAGGT CCCTCTTCTA AGACAGCCGT GGGGGAGGGA 
GCTCGCTGCA AGCGACTACT CAGCTCTGTC GAAAAGCACA AGGAAGTGTG CCACATGCTA 
AATCCACTAT CCAGCGGCTC AAACTCTTCG TCCGGGGAGG CTTCGCCTGC TGCGTCTCAC 
GTCAATGTGG CGAAATCAAA AGCTGCTGCC AGGGATGACG AATATTCGGA AGATTCGCTG 
GAGGAGTCAA CCATCTCTAC TGAACTTACA CCCGTTAAAC AGAACGGAGT CGCCTGGGAG 
ATTCACTTTA GAAACAGCAA GAAGAAATCC AGTGGCTTCA AAAATACGTC TGCATCTAAA 
ACGAAAAGCA ACAATTTAGA CGACAGTTTC ATGTACAACG AAAGAAGCAT GCTGGGCAAA 
GGGACTTTTA TTATCCGTCG CTCCACAGCC AAAAAACCGC CTCGTGTCAC AGATGTATTT 
TGTATACCAA AGCCACCACG TTCCTCGTTG GCCGAGGGAG TCGGGCCAGA GACCAAAGTT 
GGAGCCGGGG CGATCAGTAA TGAGTTCTAC ACAAGCGATA GCGAGCAAAG CGATGCCACA 
CCCCTACCAC CACCGCTGCC ACCAGCTTCA CCTGACATAA ACTTCGTGTA CAAGGAGTGT 
GAGGCTAATG TGGAGCATAC ACGCCGGGGA TTGGAGATGG TCGTCGCACA CCGAGCGATC 
TTATCTCTTG ATATCGAGGC ACCAGAACAA CTTGGCTATT GCAAGCCTGT GAAGAGTAAG 
ACGGCGATGC TAACAGAAAA GCGCCTGAGT GCCGAGTCCA TGCCGGACAA CACATCCAGC 
AGCGAAGAGT TCGAAGAGGC TCGTCTGAAC GGAATCGGCA GCTACGAGTT GTATGGTTAC 
AACGGTAACG ATCAGCGAGC TTCCAATGCC ACCACTCCGC ATACAGATTT GGCCCCAACT 
CTGGAGGAGG ACGAAGAACT TTCAGATTTA AGCTATGGGT GTGCTCCACT GATGCAAATA 
GAGCATAACA TCAGTGCTTT GTTGCGTGGA GACCTTCCAA TTATCGGCCA ACCGGCTGAG 
AGGAAAGGAG GTGGGCTTAC TGGTGCCGCA ACGACAGCTG CCGAGCAGCA TGCAAAGCGG 
GTGCTAGAAT TTCGACATGG CGTTCACAAG TCTGAAAGCG CCAAGGAGAT GCTGCTGTCA 
CAGATGCCCA GTTTGGGCCC ACTGCCACCA TCGCCGCCGA ATTCCAACCC GGATTTATTT 
GACTACAATG CCCCTCTTCC ACCGTCACCG GTGGAGCCGC GAAAGCAGCT AGAGCTCCCT 
GTAGTGCCGA ATGCCCCTCC CGCGAATCGG GGCACTACCG TAGTGGAGGT GCATGCCACC 
GCCTCTGGTA CGGCGGTGCA CAGTAGCAAC AGCCACACTC GCCGCAGTCG GGACAATATC 
GCCTCTGTGC GTCACTTTAC CGACGAGCTG CCACCGCCTC CAGCGCCTTC CCACCAATCG 
GCCAGCTTCT TAGAGGGCAA CGGCGGACCA CCTACGGTCC CCCCACATCG AGTGACTGGA 
GGTCATTCGG CTAATACAAT GAAATCCTGG AGTATCGACT CGCAATACCG CAAGAAATCG 
CCAAAGCTAT TTGGTCATTA TAGCAGCGGT GGCAGTGGGA TAACCACACC CACTGGTCTG 
GGACCGATTG CTCCACTGCC CCCTCCACAT TTCGACGGAA TGGCAGGTGG ATCTGGGTCC 
TACCATCGTC GTTATATTAA CTATGGCACC AAGCGCAGCC TAAAGCAGTC GCCGCGCGAA 
GAGCATCGCC TGCAAACCTC ATGTAGTCTT CCTGAAACGC CAATTTTTGC CAGAGGCTGT 
GACATACCAC GTACCCCATA TCGACGCCAA AATGAGCCCC TGCCTGTGGT CAGTGGCTCC 
CGCACAGCGC CCAGATCGAG CACATCGAAC AGCATCAACA TGGGCGCTTC AATTTTGGGG 
ATCGGAGGTG GTAACTATGG TACAGCCCAA ATTTGCCGTC AGCGCTCAAT AAATCATGCC 
TTGGCCTCCA ACGAAATGCT TCGCATGACC GGGGCCCCGG CTCGAGGATG GTATCCCAAG 
CAACGGGGAA TGCGACCCGT CTCCACCGAG AACATAGATC GAATGGCTTC CATGCGGGTG 
TGGGACAATC CGACGGTCAT GTCGGGCACC GGACAATCTC GTAAGCCTTT AACACTGCCA 
CCAAATCTTA CACCTTCCTT CTTGAATAAA TCACCCCGCG AGGCTCTACG GCGTGTTACT 
AGTTTGCTGA TAACCAAAAA AAAAAACAGC AAAGATCGAA AACATAAGGC GTACTGCGAT 
CAGCTTATGG ATGATAGCAA CAGCAAGCAT GTCTATGAAT TTGAATCGGG ATCCACCACG 
CGAAAGGATA CCAATCGCAA AGACAATGGA GCCTCGGGGG CGACCACGAA TAACAAACCT 
AAAAAAAAGG GGCTTTTTAA ATCTCTTTGG AAACGCTCCA AAACGGTCTC TTTAGATCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG2225 ( NM_165393.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724356.2 Drosophila melanogaster uncharacterized protein, transcript variant B (CG2225), mRNA.

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CloneID	OFa15140
Clone ID Related Accession (Same CDS sequence)	NM_001273748.1 , NM_165393.3
Accession Version	NM_165393.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165393.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAGCGG CACATGAATC GCCTAAAGCA CGCGATTCGA CCAACGTCGA GACTGAGCGG 
ACGTTTCAGC TGGAGGCAGG GCAATCCTAT GACACAATGG CAGAATCTCA TCGCGCATTC 
CATTCCCTGG AATTGGAGGC GGGAGCGGAG GTAGCCGTAG ATGCCGCTGT TGAAGCTGAC 
TCAAAAAGAC AAAACGATAA TCAACTGCAG TCGTTGGAAC CCATTTCCTT GGATGACATA 
CCCGAACCAA TAAAAGTCCT CGACGAGATT ATTTCCGAAT TTGAGGAGGC AGCAACGAAA 
CCAGTGGCCT TGAACTGCAA TAGTGGCGAG AATCAGTCCG AGGACGATGG CTACATGAGC 
CTAAGTCGCA AGAACATGTC CAAAAAGGAT TCCGAAGATG TGCCCCAAAC ACCCAGCACA 
GACACTGTGC CAGAGGGAAG CTTCAACGAA GTGGATGGTA CGGCTGAGAA TCTAACTAAA 
TTATCTGAGG TTGTACACGG ACAGGAAACT ACATCGCCTC TCATTCAGAC AACGCTGCCA 
AAGGCTAGAG GATCTAGCTC AACTCCTGCC GTAAGCTCAA ACTACTCTAG TTTACCGTGC 
TGTGGTGTCA GGGCTTCGAA TTTGGGTGGA GTTTCTGCTG CCAATTCATC AAGTGGATTG 
TCAGCCATCC GGCATCCAGG CAGCTGCAAC GGGGGTCGCA ACGCACTGGG CATCGACATT 
AGCGCTGTTC ATAACGCTGT TCTGCGTGGT AATTTGGCGA AGCTGCCGGA ACCATTCGTC 
AACGAACATC CAGTGACGAT ATACCCAGGT CCCTCTTCTA AGACAGCCGT GGGGGAGGGA 
GCTCGCTGCA AGCGACTACT CAGCTCTGTC GAAAAGCACA AGGAAGTGTG CCACATGCTA 
AATCCACTAT CCAGCGGCTC AAACTCTTCG TCCGGGGAGG CTTCGCCTGC TGCGTCTCAC 
GTCAATGTGG CGAAATCAAA AGCTGCTGCC AGGGATGACG AATATTCGGA AGATTCGCTG 
GAGGAGTCAA CCATCTCTAC TGAACTTACA CCCGTTAAAC AGAACGGAGT CGCCTGGGAG 
ATTCACTTTA GAAACAGCAA GAAGAAATCC AGTGGCTTCA AAAATACGTC TGCATCTAAA 
ACGAAAAGCA ACAATTTAGA CGACAGTTTC ATGTACAACG AAAGAAGCAT GCTGGGCAAA 
GGGACTTTTA TTATCCGTCG CTCCACAGCC AAAAAACCGC CTCGTGTCAC AGATGTATTT 
TGTATACCAA AGCCACCACG TTCCTCGTTG GCCGAGGGAG TCGGGCCAGA GACCAAAGTT 
GGAGCCGGGG CGATCAGTAA TGAGTTCTAC ACAAGCGATA GCGAGCAAAG CGATGCCACA 
CCCCTACCAC CACCGCTGCC ACCAGCTTCA CCTGACATAA ACTTCGTGTA CAAGGAGTGT 
GAGGCTAATG TGGAGCATAC ACGCCGGGGA TTGGAGATGG TCGTCGCACA CCGAGCGATC 
TTATCTCTTG ATATCGAGGC ACCAGAACAA CTTGGCTATT GCAAGCCTGT GAAGAGTAAG 
ACGGCGATGC TAACAGAAAA GCGCCTGAGT GCCGAGTCCA TGCCGGACAA CACATCCAGC 
AGCGAAGAGT TCGAAGAGGC TCGTCTGAAC GGAATCGGCA GCTACGAGTT GTATGGTTAC 
AACGGTAACG ATCAGCGAGC TTCCAATGCC ACCACTCCGC ATACAGATTT GGCCCCAACT 
CTGGAGGAGG ACGAAGAACT TTCAGATTTA AGCTATGGGT GTGCTCCACT GATGCAAATA 
GAGCATAACA TCAGTGCTTT GTTGCGTGGA GACCTTCCAA TTATCGGCCA ACCGGCTGAG 
AGGAAAGGAG GTGGGCTTAC TGGTGCCGCA ACGACAGCTG CCGAGCAGCA TGCAAAGCGG 
GTGCTAGAAT TTCGACATGG CGTTCACAAG TCTGAAAGCG CCAAGGAGAT GCTGCTGTCA 
CAGATGCCCA GTTTGGGCCC ACTGCCACCA TCGCCGCCGA ATTCCAACCC GGATTTATTT 
GACTACAATG CCCCTCTTCC ACCGTCACCG GTGGAGCCGC GAAAGCAGCT AGAGCTCCCT 
GTAGTGCCGA ATGCCCCTCC CGCGAATCGG GGCACTACCG TAGTGGAGGT GCATGCCACC 
GCCTCTGGTA CGGCGGTGCA CAGTAGCAAC AGCCACACTC GCCGCAGTCG GGACAATATC 
GCCTCTGTGC GTCACTTTAC CGACGAGCTG CCACCGCCTC CAGCGCCTTC CCACCAATCG 
GCCAGCTTCT TAGAGGGCAA CGGCGGACCA CCTACGGTCC CCCCACATCG AGTGACTGGA 
GGTCATTCGG CTAATACAAT GAAATCCTGG AGTATCGACT CGCAATACCG CAAGAAATCG 
CCAAAGCTAT TTGGTCATTA TAGCAGCGGT GGCAGTGGGA TAACCACACC CACTGGTCTG 
GGACCGATTG CTCCACTGCC CCCTCCACAT TTCGACGGAA TGGCAGGTGG ATCTGGGTCC 
TACCATCGTC GTTATATTAA CTATGGCACC AAGCGCAGCC TAAAGCAGTC GCCGCGCGAA 
GAGCATCGCC TGCAAACCTC ATGTAGTCTT CCTGAAACGC CAATTTTTGC CAGAGGCTGT 
GACATACCAC GTACCCCATA TCGACGCCAA AATGAGCCCC TGCCTGTGGT CAGTGGCTCC 
CGCACAGCGC CCAGATCGAG CACATCGAAC AGCATCAACA TGGGCGCTTC AATTTTGGGG 
ATCGGAGGTG GTAACTATGG TACAGCCCAA ATTTGCCGTC AGCGCTCAAT AAATCATGCC 
TTGGCCTCCA ACGAAATGCT TCGCATGACC GGGGCCCCGG CTCGAGGATG GTATCCCAAG 
CAACGGGGAA TGCGACCCGT CTCCACCGAG AACATAGATC GAATGGCTTC CATGCGGGTG 
TGGGACAATC CGACGGTCAT GTCGGGCACC GGACAATCTC GTAAGCCTTT AACACTGCCA 
CCAAATCTTA CACCTTCCTT CTTGAATAAA TCACCCCGCG AGGCTCTACG GCGTGTTACT 
AGTTTGCTGA TAACCAAAAA AAAAAACAGC AAAGATCGAA AACATAAGGC GTACTGCGAT 
CAGCTTATGG ATGATAGCAA CAGCAAGCAT GTCTATGAAT TTGAATCGGG ATCCACCACG 
CGAAAGGATA CCAATCGCAA AGACAATGGA GCCTCGGGGG CGACCACGAA TAACAAACCT 
AAAAAAAAGG GGCTTTTTAA ATCTCTTTGG AAACGCTCCA AAACGGTCTC TTTAGATCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724356.2
CDS	585..3887
Translation	MAAAHESPKARDSTNVETERTFQLEAGQSYDTMAESHRAFHSLELEAGAEVAVDAAVEADSKRQNDNQLQSLEPISLDDIPEPIKVLDEIISEFEEAATKPVALNCNSGENQSEDDGYMSLSRKNMSKKDSEDVPQTPSTDTVPEGSFNEVDGTAENLTKLSEVVHGQETTSPLIQTTLPKARGSSSTPAVSSNYSSLPCCGVRASNLGGVSAANSSSGLSAIRHPGSCNGGRNALGIDISAVHNAVLRGNLAKLPEPFVNEHPVTIYPGPSSKTAVGEGARCKRLLSSVEKHKEVCHMLNPLSSGSNSSSGEASPAASHVNVAKSKAAARDDEYSEDSLEESTISTELTPVKQNGVAWEIHFRNSKKKSSGFKNTSASKTKSNNLDDSFMYNERSMLGKGTFIIRRSTAKKPPRVTDVFCIPKPPRSSLAEGVGPETKVGAGAISNEFYTSDSEQSDATPLPPPLPPASPDINFVYKECEANVEHTRRGLEMVVAHRAILSLDIEAPEQLGYCKPVKSKTAMLTEKRLSAESMPDNTSSSEEFEEARLNGIGSYELYGYNGNDQRASNATTPHTDLAPTLEEDEELSDLSYGCAPLMQIEHNISALLRGDLPIIGQPAERKGGGLTGAATTAAEQHAKRVLEFRHGVHKSESAKEMLLSQMPSLGPLPPSPPNSNPDLFDYNAPLPPSPVEPRKQLELPVVPNAPPANRGTTVVEVHATASGTAVHSSNSHTRRSRDNIASVRHFTDELPPPPAPSHQSASFLEGNGGPPTVPPHRVTGGHSANTMKSWSIDSQYRKKSPKLFGHYSSGGSGITTPTGLGPIAPLPPPHFDGMAGGSGSYHRRYINYGTKRSLKQSPREEHRLQTSCSLPETPIFARGCDIPRTPYRRQNEPLPVVSGSRTAPRSSTSNSINMGASILGIGGGNYGTAQICRQRSINHALASNEMLRMTGAPARGWYPKQRGMRPVSTENIDRMASMRVWDNPTVMSGTGQSRKPLTLPPNLTPSFLNKSPREALRRVTSLLITKKKNSKDRKHKAYCDQLMDDSNSKHVYEFESGSTTRKDTNRKDNGASGATTNNKPKKKGLFKSLWKRSKTVSLDQ

Target ORF information:

RefSeq Version	NM_165393.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG2225), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165393.3

ORF Insert Sequence:

ATGGCAGCGG CACATGAATC GCCTAAAGCA CGCGATTCGA CCAACGTCGA GACTGAGCGG 
ACGTTTCAGC TGGAGGCAGG GCAATCCTAT GACACAATGG CAGAATCTCA TCGCGCATTC 
CATTCCCTGG AATTGGAGGC GGGAGCGGAG GTAGCCGTAG ATGCCGCTGT TGAAGCTGAC 
TCAAAAAGAC AAAACGATAA TCAACTGCAG TCGTTGGAAC CCATTTCCTT GGATGACATA 
CCCGAACCAA TAAAAGTCCT CGACGAGATT ATTTCCGAAT TTGAGGAGGC AGCAACGAAA 
CCAGTGGCCT TGAACTGCAA TAGTGGCGAG AATCAGTCCG AGGACGATGG CTACATGAGC 
CTAAGTCGCA AGAACATGTC CAAAAAGGAT TCCGAAGATG TGCCCCAAAC ACCCAGCACA 
GACACTGTGC CAGAGGGAAG CTTCAACGAA GTGGATGGTA CGGCTGAGAA TCTAACTAAA 
TTATCTGAGG TTGTACACGG ACAGGAAACT ACATCGCCTC TCATTCAGAC AACGCTGCCA 
AAGGCTAGAG GATCTAGCTC AACTCCTGCC GTAAGCTCAA ACTACTCTAG TTTACCGTGC 
TGTGGTGTCA GGGCTTCGAA TTTGGGTGGA GTTTCTGCTG CCAATTCATC AAGTGGATTG 
TCAGCCATCC GGCATCCAGG CAGCTGCAAC GGGGGTCGCA ACGCACTGGG CATCGACATT 
AGCGCTGTTC ATAACGCTGT TCTGCGTGGT AATTTGGCGA AGCTGCCGGA ACCATTCGTC 
AACGAACATC CAGTGACGAT ATACCCAGGT CCCTCTTCTA AGACAGCCGT GGGGGAGGGA 
GCTCGCTGCA AGCGACTACT CAGCTCTGTC GAAAAGCACA AGGAAGTGTG CCACATGCTA 
AATCCACTAT CCAGCGGCTC AAACTCTTCG TCCGGGGAGG CTTCGCCTGC TGCGTCTCAC 
GTCAATGTGG CGAAATCAAA AGCTGCTGCC AGGGATGACG AATATTCGGA AGATTCGCTG 
GAGGAGTCAA CCATCTCTAC TGAACTTACA CCCGTTAAAC AGAACGGAGT CGCCTGGGAG 
ATTCACTTTA GAAACAGCAA GAAGAAATCC AGTGGCTTCA AAAATACGTC TGCATCTAAA 
ACGAAAAGCA ACAATTTAGA CGACAGTTTC ATGTACAACG AAAGAAGCAT GCTGGGCAAA 
GGGACTTTTA TTATCCGTCG CTCCACAGCC AAAAAACCGC CTCGTGTCAC AGATGTATTT 
TGTATACCAA AGCCACCACG TTCCTCGTTG GCCGAGGGAG TCGGGCCAGA GACCAAAGTT 
GGAGCCGGGG CGATCAGTAA TGAGTTCTAC ACAAGCGATA GCGAGCAAAG CGATGCCACA 
CCCCTACCAC CACCGCTGCC ACCAGCTTCA CCTGACATAA ACTTCGTGTA CAAGGAGTGT 
GAGGCTAATG TGGAGCATAC ACGCCGGGGA TTGGAGATGG TCGTCGCACA CCGAGCGATC 
TTATCTCTTG ATATCGAGGC ACCAGAACAA CTTGGCTATT GCAAGCCTGT GAAGAGTAAG 
ACGGCGATGC TAACAGAAAA GCGCCTGAGT GCCGAGTCCA TGCCGGACAA CACATCCAGC 
AGCGAAGAGT TCGAAGAGGC TCGTCTGAAC GGAATCGGCA GCTACGAGTT GTATGGTTAC 
AACGGTAACG ATCAGCGAGC TTCCAATGCC ACCACTCCGC ATACAGATTT GGCCCCAACT 
CTGGAGGAGG ACGAAGAACT TTCAGATTTA AGCTATGGGT GTGCTCCACT GATGCAAATA 
GAGCATAACA TCAGTGCTTT GTTGCGTGGA GACCTTCCAA TTATCGGCCA ACCGGCTGAG 
AGGAAAGGAG GTGGGCTTAC TGGTGCCGCA ACGACAGCTG CCGAGCAGCA TGCAAAGCGG 
GTGCTAGAAT TTCGACATGG CGTTCACAAG TCTGAAAGCG CCAAGGAGAT GCTGCTGTCA 
CAGATGCCCA GTTTGGGCCC ACTGCCACCA TCGCCGCCGA ATTCCAACCC GGATTTATTT 
GACTACAATG CCCCTCTTCC ACCGTCACCG GTGGAGCCGC GAAAGCAGCT AGAGCTCCCT 
GTAGTGCCGA ATGCCCCTCC CGCGAATCGG GGCACTACCG TAGTGGAGGT GCATGCCACC 
GCCTCTGGTA CGGCGGTGCA CAGTAGCAAC AGCCACACTC GCCGCAGTCG GGACAATATC 
GCCTCTGTGC GTCACTTTAC CGACGAGCTG CCACCGCCTC CAGCGCCTTC CCACCAATCG 
GCCAGCTTCT TAGAGGGCAA CGGCGGACCA CCTACGGTCC CCCCACATCG AGTGACTGGA 
GGTCATTCGG CTAATACAAT GAAATCCTGG AGTATCGACT CGCAATACCG CAAGAAATCG 
CCAAAGCTAT TTGGTCATTA TAGCAGCGGT GGCAGTGGGA TAACCACACC CACTGGTCTG 
GGACCGATTG CTCCACTGCC CCCTCCACAT TTCGACGGAA TGGCAGGTGG ATCTGGGTCC 
TACCATCGTC GTTATATTAA CTATGGCACC AAGCGCAGCC TAAAGCAGTC GCCGCGCGAA 
GAGCATCGCC TGCAAACCTC ATGTAGTCTT CCTGAAACGC CAATTTTTGC CAGAGGCTGT 
GACATACCAC GTACCCCATA TCGACGCCAA AATGAGCCCC TGCCTGTGGT CAGTGGCTCC 
CGCACAGCGC CCAGATCGAG CACATCGAAC AGCATCAACA TGGGCGCTTC AATTTTGGGG 
ATCGGAGGTG GTAACTATGG TACAGCCCAA ATTTGCCGTC AGCGCTCAAT AAATCATGCC 
TTGGCCTCCA ACGAAATGCT TCGCATGACC GGGGCCCCGG CTCGAGGATG GTATCCCAAG 
CAACGGGGAA TGCGACCCGT CTCCACCGAG AACATAGATC GAATGGCTTC CATGCGGGTG 
TGGGACAATC CGACGGTCAT GTCGGGCACC GGACAATCTC GTAAGCCTTT AACACTGCCA 
CCAAATCTTA CACCTTCCTT CTTGAATAAA TCACCCCGCG AGGCTCTACG GCGTGTTACT 
AGTTTGCTGA TAACCAAAAA AAAAAACAGC AAAGATCGAA AACATAAGGC GTACTGCGAT 
CAGCTTATGG ATGATAGCAA CAGCAAGCAT GTCTATGAAT TTGAATCGGG ATCCACCACG 
CGAAAGGATA CCAATCGCAA AGACAATGGA GCCTCGGGGG CGACCACGAA TAACAAACCT 
AAAAAAAAGG GGCTTTTTAA ATCTCTTTGG AAACGCTCCA AAACGGTCTC TTTAGATCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG2225 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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