Rhau cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rhau
Entrez Gene ID	35339
Full Name	CG9323 gene product from transcript CG9323-RB
Synonyms	38E.15,Dmel\CG9323,cg9323
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 38E7-38E7

mRNA and Protein(s)

mRNA	Protein	Name
NM_136212.2	NP_610056.1	RHAU helicase, isoform A
NM_001273710.1	NP_001260639.1	RHAU helicase, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	DHX36	NP_065916.2
Arabidopsis thaliana (thale cress)	AT2G35920	NP_850255.1
Arabidopsis thaliana (thale cress)	AT1G48650	NP_175298.2
Bos taurus (cattle)	DHX36	NP_001073720.1
Rattus norvegicus (Norway rat)	Dhx36	NP_001101148.1
Drosophila melanogaster (fruit fly)	CG9323	NP_001260639.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008239	XP_317231.4
Arabidopsis thaliana (thale cress)	AT2G01130	NP_178223.2
Xenopus tropicalis (tropical clawed frog)	dhx36	XP_002933292.1
Mus musculus (house mouse)	Dhx36	NP_082412.2
Arabidopsis thaliana (thale cress)	AT5G04895	NP_680142.2
	DHX36	XP_001106252.1
Gallus gallus (chicken)	DHX36	XP_422834.3
Danio rerio (zebrafish)	si:dkey-119o24.1	NP_001122016.1
Pan troglodytes (chimpanzee)	DHX36	XP_001147599.1
Canis lupus familiaris (dog)	DHX36	XP_534311.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The study provides the first evidence that the unfolding kinetics of a G-quadruplex can be modulated by different nucleotide-bound states of the RHAU helicase (CG9323).
Title: RHAU helicase stabilizes G4 in its nucleotide-free state and destabilizes G4 upon ATP hydrolysis.

CG9323 is the true ortholog of the human RHAU/DHX36 DEAH-box RNA helicase. CG9323 possesses guanine quadruplex-RNA binding and resolving activities.
Title: Role of the amino terminal RHAU-specific motif in the recognition and resolution of guanine quadruplex-RNA by the DEAH-box RNA helicase RHAU.

The following Rhau gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rhau cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa15058	NM_001273710.1 Latest version!	Drosophila melanogaster RHAU helicase (Rhau), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa15058	NM_136212.2 Latest version!	Drosophila melanogaster RHAU helicase (Rhau), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rhau ( NM_136212.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610056.1 Drosophila melanogaster RHAU helicase (Rhau), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa15058
Clone ID Related Accession (Same CDS sequence)	NM_136212.2 , NM_001273710.1
Accession Version	NM_136212.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	RHAU helicase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_136212.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGCGCG ATAGGGACTC ATCCGGCTCC AACGCCCGCA AGGGCAACCG CCCACCGGGT 
CTCAGGGGCA AGGACATCGG TTTGTACTAT CGCAATCTTG CCCGGCAGCA AAAGAAGGAC 
AGAGGCGAGA ATGCGGAATC AAAGGAGCCG CAAATCCGCT TGGGCTGCAA TGTCAGTGCT 
CCCAGTGGAG TGCTTGAGCG TGTGAAGGAG TTAATGGAGG ACTACAGCCG GGCACCGTCC 
AGACAAAATG TGGATGACAA AAATGTGGAT GCCAAATTTC AGCAACAGTT TCGCCATCTT 
TTGAGTGTAA ACTTTGAGGA ATTCGTTGCG GAGACCAAGG AACGGAACGC GGACTTAGAC 
TGGGTAAACC CCAAGCTGGA CGAGAGACTG CAGCTGGAAC TGGGGCAACG CCAGTTGGAG 
GAGAATGCCA AGAAACGGCT AGAAGCACGG AAAAAACTGC CCACCATGAA ATACGCCGAC 
GATATTATCC AGGCTGTGCG GGAAAACCAA GTAATACTCA TTGTAGGAAG CACTGGATGT 
GGCAAGACCA CTCAGGTGCC TCAAATCCTC CTCGACGACG CCATTTCCCG CGGATGTGCC 
TCGTCGTGCC GCATCATTTG CACTCAGCCA CGAAGGATTT CAGCCATCGC CATTGCCGAG 
TGGGTGAGTT ACGAGCGCTG CGAGAGCCTG GGCAATTCGG TAGGCTACCA GATTCGCCTA 
GAGAGCAGGA AGGCCAGGGA GAGAGCCTCC ATCACTTACT GCACCACAGG CGTTCTACTG 
CAGCAGCTTC AATCGGACCC ACTGATGCAC AATCTAAGTG TCTTAATCCT GGATGAGATC 
CACGAACGCA GCGTGGAAAC AGATCTCCTA ATGGGTCTTC TAAAGGTTAT CCTGCCCCAC 
AGACCCGATC TAAAGGTGAT CCTGATGAGT GCAACTGTAC GGGAGCAGGA TTTTTGTGAC 
TACTTTAACA ACTGTCCCAT GTTCCGCATC GAGGGCGTGA TGTTTCCCGT GAAGATGCTC 
TACTTGGAGG ATGTTCTGTC CAAGACGAAC TACGAGTTCC AAAAGTTCCG CGATCGTCGT 
CCCAAGCGGG ATCCGCCTGA GCGCAGGATG AAGCACGAGG CTATGATAGA ACCGTATCTG 
CGACGCATCA GGAACTCGTA CGATAGTCGT GTGCTCGACA AGCTGCGTTT GCCGGAGTCG 
GAGGGCTGCG AGGATATTGA TTTTATAGCC GATTTGGTTT ACTACATATG CGAAAACGAA 
CCAGAGGGTG CCATATTAGT GTTCCTGCCT GGTTATGATA AGATCTCCCA ACTGTACAAT 
ATTCTGGATA AACCAAAGAC TTCGAAGGGC CAAAGGTGGC GGGATCATAT GGCCGTATTT 
CCATTACATT CCCTAATGCA GTCAGGGGAA CAGCAGGCAG TGTTCAGGCG ACCACCGGCG 
GGCCAGCGTA AGGTTATAAT CTCTACAATT ATCGCCGAAA CGTCGGTGAC TATTGACGAC 
GTGGTTTATG TGATAAACTC TGGCCGGACG AAAGCCACCA ACTATGACAT CGAAACGAAC 
ATACAGTCGC TGGATGAGGT TTGGGTGACC AAAGCAAATA CCCAGCAGCG GAGAGGCAGG 
GCAGGTCGAG TGCGGCCGGG CATATGCTAT AATCTGTTCT CCCGCGCTAG AGAGGATCGC 
ATGGACGATA TTCCGACGCC GGAGATACTA CGCTCCAAGC TGGAATCCAT AATTTTAAGC 
CTCAAGCTAC TGCACATCGA CGATCCGTAT CGGTTCCTGC AAACTCTTAT TAATGCCCCC 
AATCCCGAGG CCATAAAGAT GGGAGTGGAA CTGCTAAAGC GCATTGAGGC GTTGGACCAG 
ACGGGCACCC TTACTCCACT GGGCATGCAC TTGGCAAAGC TGCCCATTGA TCCGCAGATG 
GGAAAAATGA TCCTGATGTC GGCGCTCTTC TGTTGCTTGG ATCCCATCAC TTCGGCGGCG 
GCTGCCTTGT CCTTTAAGTC CCCCTTCTAC TCGCCTTTGG GTAAAGAGAG CCGAGTGGAT 
GAAATCAAGC GGAGAATGGC CCGAAATATG CGGAGCGATC ACCTGATGGT GCACAATACC 
ATTATTGCGT ACCGCGATAG CCGCTATTCG CACGCGGAAC GAGACTTCTG CTATAAGAAT 
TTCCTCAGCT CCATGACACT GCAGCAGTTG GAGAGGATGA AGAATCAGTT CAGTGAGCTG 
CTCTACAACT ACAAATTTCT GGCATCGTCA AACTGCAAGG ATGCTGCATC CAATAAGAAT 
TCTGAGAAGA TTCCCCTACT GAGGGCCATT ATAGGAGCGG GACTCTATCC CAACATGGCT 
CACTTGCGAA AATCACGGCA GATCAAGAAT AGAGTACGCG CTATCCACAC CATGGCAACG 
GACGATGGAC GCCGCGTTAA CTTTCATCCC TCCTCGGTGA ACAGCGGAGA GAGTGGCTTC 
GACTCGGCAT ACTTTGTCTA CTTTCAGCGG CAGAAGTCCA CCGATCTTTT CCTGCTCGAC 
TCCACAATGG TTTTTCCCAT GGCACTGATC ATTTTCGGAG ATGGCGTGGA AGCGGGAGTG 
ACCCAAAATA CGCCATATCT GTGCGTGGCC AAGACATATT ACTTTAAATG CAATCGTGAA 
ACTGCCGACG TAGTCATACA ATTAAGAAGC AATTTGGAGA AACTGCTGCT CAAAAAGGCT 
TTGTATCCGG CCCCGATCGA GGAGAACGGA TATGAAAAGC AGTTGATCAA AGCCATCGAA 
TTGCTTCTCT CGCTAGACGA AAGACTCGGC GAGGACTATA TTTCATCCGA TGAAATCGAT 
GACATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610056.1
CDS	98..2926
Translation	MQRDRDSSGSNARKGNRPPGLRGKDIGLYYRNLARQQKKDRGENAESKEPQIRLGCNVSAPSGVLERVKELMEDYSRAPSRQNVDDKNVDAKFQQQFRHLLSVNFEEFVAETKERNADLDWVNPKLDERLQLELGQRQLEENAKKRLEARKKLPTMKYADDIIQAVRENQVILIVGSTGCGKTTQVPQILLDDAISRGCASSCRIICTQPRRISAIAIAEWVSYERCESLGNSVGYQIRLESRKARERASITYCTTGVLLQQLQSDPLMHNLSVLILDEIHERSVETDLLMGLLKVILPHRPDLKVILMSATVREQDFCDYFNNCPMFRIEGVMFPVKMLYLEDVLSKTNYEFQKFRDRRPKRDPPERRMKHEAMIEPYLRRIRNSYDSRVLDKLRLPESEGCEDIDFIADLVYYICENEPEGAILVFLPGYDKISQLYNILDKPKTSKGQRWRDHMAVFPLHSLMQSGEQQAVFRRPPAGQRKVIISTIIAETSVTIDDVVYVINSGRTKATNYDIETNIQSLDEVWVTKANTQQRRGRAGRVRPGICYNLFSRAREDRMDDIPTPEILRSKLESIILSLKLLHIDDPYRFLQTLINAPNPEAIKMGVELLKRIEALDQTGTLTPLGMHLAKLPIDPQMGKMILMSALFCCLDPITSAAAALSFKSPFYSPLGKESRVDEIKRRMARNMRSDHLMVHNTIIAYRDSRYSHAERDFCYKNFLSSMTLQQLERMKNQFSELLYNYKFLASSNCKDAASNKNSEKIPLLRAIIGAGLYPNMAHLRKSRQIKNRVRAIHTMATDDGRRVNFHPSSVNSGESGFDSAYFVYFQRQKSTDLFLLDSTMVFPMALIIFGDGVEAGVTQNTPYLCVAKTYYFKCNRETADVVIQLRSNLEKLLLKKALYPAPIEENGYEKQLIKAIELLLSLDERLGEDYISSDEIDDI

Target ORF information:

RefSeq Version	NM_136212.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster RHAU helicase (Rhau), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136212.2

ORF Insert Sequence:

ATGCAGCGCG ATAGGGACTC ATCCGGCTCC AACGCCCGCA AGGGCAACCG CCCACCGGGT 
CTCAGGGGCA AGGACATCGG TTTGTACTAT CGCAATCTTG CCCGGCAGCA AAAGAAGGAC 
AGAGGCGAGA ATGCGGAATC AAAGGAGCCG CAAATCCGCT TGGGCTGCAA TGTCAGTGCT 
CCCAGTGGAG TGCTTGAGCG TGTGAAGGAG TTAATGGAGG ACTACAGCCG GGCACCGTCC 
AGACAAAATG TGGATGACAA AAATGTGGAT GCCAAATTTC AGCAACAGTT TCGCCATCTT 
TTGAGTGTAA ACTTTGAGGA ATTCGTTGCG GAGACCAAGG AACGGAACGC GGACTTAGAC 
TGGGTAAACC CCAAGCTGGA CGAGAGACTG CAGCTGGAAC TGGGGCAACG CCAGTTGGAG 
GAGAATGCCA AGAAACGGCT AGAAGCACGG AAAAAACTGC CCACCATGAA ATACGCCGAC 
GATATTATCC AGGCTGTGCG GGAAAACCAA GTAATACTCA TTGTAGGAAG CACTGGATGT 
GGCAAGACCA CTCAGGTGCC TCAAATCCTC CTCGACGACG CCATTTCCCG CGGATGTGCC 
TCGTCGTGCC GCATCATTTG CACTCAGCCA CGAAGGATTT CAGCCATCGC CATTGCCGAG 
TGGGTGAGTT ACGAGCGCTG CGAGAGCCTG GGCAATTCGG TAGGCTACCA GATTCGCCTA 
GAGAGCAGGA AGGCCAGGGA GAGAGCCTCC ATCACTTACT GCACCACAGG CGTTCTACTG 
CAGCAGCTTC AATCGGACCC ACTGATGCAC AATCTAAGTG TCTTAATCCT GGATGAGATC 
CACGAACGCA GCGTGGAAAC AGATCTCCTA ATGGGTCTTC TAAAGGTTAT CCTGCCCCAC 
AGACCCGATC TAAAGGTGAT CCTGATGAGT GCAACTGTAC GGGAGCAGGA TTTTTGTGAC 
TACTTTAACA ACTGTCCCAT GTTCCGCATC GAGGGCGTGA TGTTTCCCGT GAAGATGCTC 
TACTTGGAGG ATGTTCTGTC CAAGACGAAC TACGAGTTCC AAAAGTTCCG CGATCGTCGT 
CCCAAGCGGG ATCCGCCTGA GCGCAGGATG AAGCACGAGG CTATGATAGA ACCGTATCTG 
CGACGCATCA GGAACTCGTA CGATAGTCGT GTGCTCGACA AGCTGCGTTT GCCGGAGTCG 
GAGGGCTGCG AGGATATTGA TTTTATAGCC GATTTGGTTT ACTACATATG CGAAAACGAA 
CCAGAGGGTG CCATATTAGT GTTCCTGCCT GGTTATGATA AGATCTCCCA ACTGTACAAT 
ATTCTGGATA AACCAAAGAC TTCGAAGGGC CAAAGGTGGC GGGATCATAT GGCCGTATTT 
CCATTACATT CCCTAATGCA GTCAGGGGAA CAGCAGGCAG TGTTCAGGCG ACCACCGGCG 
GGCCAGCGTA AGGTTATAAT CTCTACAATT ATCGCCGAAA CGTCGGTGAC TATTGACGAC 
GTGGTTTATG TGATAAACTC TGGCCGGACG AAAGCCACCA ACTATGACAT CGAAACGAAC 
ATACAGTCGC TGGATGAGGT TTGGGTGACC AAAGCAAATA CCCAGCAGCG GAGAGGCAGG 
GCAGGTCGAG TGCGGCCGGG CATATGCTAT AATCTGTTCT CCCGCGCTAG AGAGGATCGC 
ATGGACGATA TTCCGACGCC GGAGATACTA CGCTCCAAGC TGGAATCCAT AATTTTAAGC 
CTCAAGCTAC TGCACATCGA CGATCCGTAT CGGTTCCTGC AAACTCTTAT TAATGCCCCC 
AATCCCGAGG CCATAAAGAT GGGAGTGGAA CTGCTAAAGC GCATTGAGGC GTTGGACCAG 
ACGGGCACCC TTACTCCACT GGGCATGCAC TTGGCAAAGC TGCCCATTGA TCCGCAGATG 
GGAAAAATGA TCCTGATGTC GGCGCTCTTC TGTTGCTTGG ATCCCATCAC TTCGGCGGCG 
GCTGCCTTGT CCTTTAAGTC CCCCTTCTAC TCGCCTTTGG GTAAAGAGAG CCGAGTGGAT 
GAAATCAAGC GGAGAATGGC CCGAAATATG CGGAGCGATC ACCTGATGGT GCACAATACC 
ATTATTGCGT ACCGCGATAG CCGCTATTCG CACGCGGAAC GAGACTTCTG CTATAAGAAT 
TTCCTCAGCT CCATGACACT GCAGCAGTTG GAGAGGATGA AGAATCAGTT CAGTGAGCTG 
CTCTACAACT ACAAATTTCT GGCATCGTCA AACTGCAAGG ATGCTGCATC CAATAAGAAT 
TCTGAGAAGA TTCCCCTACT GAGGGCCATT ATAGGAGCGG GACTCTATCC CAACATGGCT 
CACTTGCGAA AATCACGGCA GATCAAGAAT AGAGTACGCG CTATCCACAC CATGGCAACG 
GACGATGGAC GCCGCGTTAA CTTTCATCCC TCCTCGGTGA ACAGCGGAGA GAGTGGCTTC 
GACTCGGCAT ACTTTGTCTA CTTTCAGCGG CAGAAGTCCA CCGATCTTTT CCTGCTCGAC 
TCCACAATGG TTTTTCCCAT GGCACTGATC ATTTTCGGAG ATGGCGTGGA AGCGGGAGTG 
ACCCAAAATA CGCCATATCT GTGCGTGGCC AAGACATATT ACTTTAAATG CAATCGTGAA 
ACTGCCGACG TAGTCATACA ATTAAGAAGC AATTTGGAGA AACTGCTGCT CAAAAAGGCT 
TTGTATCCGG CCCCGATCGA GGAGAACGGA TATGAAAAGC AGTTGATCAA AGCCATCGAA 
TTGCTTCTCT CGCTAGACGA AAGACTCGGC GAGGACTATA TTTCATCCGA TGAAATCGAT 
GACATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhau ( NM_001273710.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260639.1 Drosophila melanogaster RHAU helicase (Rhau), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa15058
Clone ID Related Accession (Same CDS sequence)	NM_136212.2 , NM_001273710.1
Accession Version	NM_001273710.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	RHAU helicase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGCGCG ATAGGGACTC ATCCGGCTCC AACGCCCGCA AGGGCAACCG CCCACCGGGT 
CTCAGGGGCA AGGACATCGG TTTGTACTAT CGCAATCTTG CCCGGCAGCA AAAGAAGGAC 
AGAGGCGAGA ATGCGGAATC AAAGGAGCCG CAAATCCGCT TGGGCTGCAA TGTCAGTGCT 
CCCAGTGGAG TGCTTGAGCG TGTGAAGGAG TTAATGGAGG ACTACAGCCG GGCACCGTCC 
AGACAAAATG TGGATGACAA AAATGTGGAT GCCAAATTTC AGCAACAGTT TCGCCATCTT 
TTGAGTGTAA ACTTTGAGGA ATTCGTTGCG GAGACCAAGG AACGGAACGC GGACTTAGAC 
TGGGTAAACC CCAAGCTGGA CGAGAGACTG CAGCTGGAAC TGGGGCAACG CCAGTTGGAG 
GAGAATGCCA AGAAACGGCT AGAAGCACGG AAAAAACTGC CCACCATGAA ATACGCCGAC 
GATATTATCC AGGCTGTGCG GGAAAACCAA GTAATACTCA TTGTAGGAAG CACTGGATGT 
GGCAAGACCA CTCAGGTGCC TCAAATCCTC CTCGACGACG CCATTTCCCG CGGATGTGCC 
TCGTCGTGCC GCATCATTTG CACTCAGCCA CGAAGGATTT CAGCCATCGC CATTGCCGAG 
TGGGTGAGTT ACGAGCGCTG CGAGAGCCTG GGCAATTCGG TAGGCTACCA GATTCGCCTA 
GAGAGCAGGA AGGCCAGGGA GAGAGCCTCC ATCACTTACT GCACCACAGG CGTTCTACTG 
CAGCAGCTTC AATCGGACCC ACTGATGCAC AATCTAAGTG TCTTAATCCT GGATGAGATC 
CACGAACGCA GCGTGGAAAC AGATCTCCTA ATGGGTCTTC TAAAGGTTAT CCTGCCCCAC 
AGACCCGATC TAAAGGTGAT CCTGATGAGT GCAACTGTAC GGGAGCAGGA TTTTTGTGAC 
TACTTTAACA ACTGTCCCAT GTTCCGCATC GAGGGCGTGA TGTTTCCCGT GAAGATGCTC 
TACTTGGAGG ATGTTCTGTC CAAGACGAAC TACGAGTTCC AAAAGTTCCG CGATCGTCGT 
CCCAAGCGGG ATCCGCCTGA GCGCAGGATG AAGCACGAGG CTATGATAGA ACCGTATCTG 
CGACGCATCA GGAACTCGTA CGATAGTCGT GTGCTCGACA AGCTGCGTTT GCCGGAGTCG 
GAGGGCTGCG AGGATATTGA TTTTATAGCC GATTTGGTTT ACTACATATG CGAAAACGAA 
CCAGAGGGTG CCATATTAGT GTTCCTGCCT GGTTATGATA AGATCTCCCA ACTGTACAAT 
ATTCTGGATA AACCAAAGAC TTCGAAGGGC CAAAGGTGGC GGGATCATAT GGCCGTATTT 
CCATTACATT CCCTAATGCA GTCAGGGGAA CAGCAGGCAG TGTTCAGGCG ACCACCGGCG 
GGCCAGCGTA AGGTTATAAT CTCTACAATT ATCGCCGAAA CGTCGGTGAC TATTGACGAC 
GTGGTTTATG TGATAAACTC TGGCCGGACG AAAGCCACCA ACTATGACAT CGAAACGAAC 
ATACAGTCGC TGGATGAGGT TTGGGTGACC AAAGCAAATA CCCAGCAGCG GAGAGGCAGG 
GCAGGTCGAG TGCGGCCGGG CATATGCTAT AATCTGTTCT CCCGCGCTAG AGAGGATCGC 
ATGGACGATA TTCCGACGCC GGAGATACTA CGCTCCAAGC TGGAATCCAT AATTTTAAGC 
CTCAAGCTAC TGCACATCGA CGATCCGTAT CGGTTCCTGC AAACTCTTAT TAATGCCCCC 
AATCCCGAGG CCATAAAGAT GGGAGTGGAA CTGCTAAAGC GCATTGAGGC GTTGGACCAG 
ACGGGCACCC TTACTCCACT GGGCATGCAC TTGGCAAAGC TGCCCATTGA TCCGCAGATG 
GGAAAAATGA TCCTGATGTC GGCGCTCTTC TGTTGCTTGG ATCCCATCAC TTCGGCGGCG 
GCTGCCTTGT CCTTTAAGTC CCCCTTCTAC TCGCCTTTGG GTAAAGAGAG CCGAGTGGAT 
GAAATCAAGC GGAGAATGGC CCGAAATATG CGGAGCGATC ACCTGATGGT GCACAATACC 
ATTATTGCGT ACCGCGATAG CCGCTATTCG CACGCGGAAC GAGACTTCTG CTATAAGAAT 
TTCCTCAGCT CCATGACACT GCAGCAGTTG GAGAGGATGA AGAATCAGTT CAGTGAGCTG 
CTCTACAACT ACAAATTTCT GGCATCGTCA AACTGCAAGG ATGCTGCATC CAATAAGAAT 
TCTGAGAAGA TTCCCCTACT GAGGGCCATT ATAGGAGCGG GACTCTATCC CAACATGGCT 
CACTTGCGAA AATCACGGCA GATCAAGAAT AGAGTACGCG CTATCCACAC CATGGCAACG 
GACGATGGAC GCCGCGTTAA CTTTCATCCC TCCTCGGTGA ACAGCGGAGA GAGTGGCTTC 
GACTCGGCAT ACTTTGTCTA CTTTCAGCGG CAGAAGTCCA CCGATCTTTT CCTGCTCGAC 
TCCACAATGG TTTTTCCCAT GGCACTGATC ATTTTCGGAG ATGGCGTGGA AGCGGGAGTG 
ACCCAAAATA CGCCATATCT GTGCGTGGCC AAGACATATT ACTTTAAATG CAATCGTGAA 
ACTGCCGACG TAGTCATACA ATTAAGAAGC AATTTGGAGA AACTGCTGCT CAAAAAGGCT 
TTGTATCCGG CCCCGATCGA GGAGAACGGA TATGAAAAGC AGTTGATCAA AGCCATCGAA 
TTGCTTCTCT CGCTAGACGA AAGACTCGGC GAGGACTATA TTTCATCCGA TGAAATCGAT 
GACATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260639.1
CDS	204..3032
Translation	MQRDRDSSGSNARKGNRPPGLRGKDIGLYYRNLARQQKKDRGENAESKEPQIRLGCNVSAPSGVLERVKELMEDYSRAPSRQNVDDKNVDAKFQQQFRHLLSVNFEEFVAETKERNADLDWVNPKLDERLQLELGQRQLEENAKKRLEARKKLPTMKYADDIIQAVRENQVILIVGSTGCGKTTQVPQILLDDAISRGCASSCRIICTQPRRISAIAIAEWVSYERCESLGNSVGYQIRLESRKARERASITYCTTGVLLQQLQSDPLMHNLSVLILDEIHERSVETDLLMGLLKVILPHRPDLKVILMSATVREQDFCDYFNNCPMFRIEGVMFPVKMLYLEDVLSKTNYEFQKFRDRRPKRDPPERRMKHEAMIEPYLRRIRNSYDSRVLDKLRLPESEGCEDIDFIADLVYYICENEPEGAILVFLPGYDKISQLYNILDKPKTSKGQRWRDHMAVFPLHSLMQSGEQQAVFRRPPAGQRKVIISTIIAETSVTIDDVVYVINSGRTKATNYDIETNIQSLDEVWVTKANTQQRRGRAGRVRPGICYNLFSRAREDRMDDIPTPEILRSKLESIILSLKLLHIDDPYRFLQTLINAPNPEAIKMGVELLKRIEALDQTGTLTPLGMHLAKLPIDPQMGKMILMSALFCCLDPITSAAAALSFKSPFYSPLGKESRVDEIKRRMARNMRSDHLMVHNTIIAYRDSRYSHAERDFCYKNFLSSMTLQQLERMKNQFSELLYNYKFLASSNCKDAASNKNSEKIPLLRAIIGAGLYPNMAHLRKSRQIKNRVRAIHTMATDDGRRVNFHPSSVNSGESGFDSAYFVYFQRQKSTDLFLLDSTMVFPMALIIFGDGVEAGVTQNTPYLCVAKTYYFKCNRETADVVIQLRSNLEKLLLKKALYPAPIEENGYEKQLIKAIELLLSLDERLGEDYISSDEIDDI

Target ORF information:

RefSeq Version	NM_001273710.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster RHAU helicase (Rhau), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273710.1

ORF Insert Sequence:

ATGCAGCGCG ATAGGGACTC ATCCGGCTCC AACGCCCGCA AGGGCAACCG CCCACCGGGT 
CTCAGGGGCA AGGACATCGG TTTGTACTAT CGCAATCTTG CCCGGCAGCA AAAGAAGGAC 
AGAGGCGAGA ATGCGGAATC AAAGGAGCCG CAAATCCGCT TGGGCTGCAA TGTCAGTGCT 
CCCAGTGGAG TGCTTGAGCG TGTGAAGGAG TTAATGGAGG ACTACAGCCG GGCACCGTCC 
AGACAAAATG TGGATGACAA AAATGTGGAT GCCAAATTTC AGCAACAGTT TCGCCATCTT 
TTGAGTGTAA ACTTTGAGGA ATTCGTTGCG GAGACCAAGG AACGGAACGC GGACTTAGAC 
TGGGTAAACC CCAAGCTGGA CGAGAGACTG CAGCTGGAAC TGGGGCAACG CCAGTTGGAG 
GAGAATGCCA AGAAACGGCT AGAAGCACGG AAAAAACTGC CCACCATGAA ATACGCCGAC 
GATATTATCC AGGCTGTGCG GGAAAACCAA GTAATACTCA TTGTAGGAAG CACTGGATGT 
GGCAAGACCA CTCAGGTGCC TCAAATCCTC CTCGACGACG CCATTTCCCG CGGATGTGCC 
TCGTCGTGCC GCATCATTTG CACTCAGCCA CGAAGGATTT CAGCCATCGC CATTGCCGAG 
TGGGTGAGTT ACGAGCGCTG CGAGAGCCTG GGCAATTCGG TAGGCTACCA GATTCGCCTA 
GAGAGCAGGA AGGCCAGGGA GAGAGCCTCC ATCACTTACT GCACCACAGG CGTTCTACTG 
CAGCAGCTTC AATCGGACCC ACTGATGCAC AATCTAAGTG TCTTAATCCT GGATGAGATC 
CACGAACGCA GCGTGGAAAC AGATCTCCTA ATGGGTCTTC TAAAGGTTAT CCTGCCCCAC 
AGACCCGATC TAAAGGTGAT CCTGATGAGT GCAACTGTAC GGGAGCAGGA TTTTTGTGAC 
TACTTTAACA ACTGTCCCAT GTTCCGCATC GAGGGCGTGA TGTTTCCCGT GAAGATGCTC 
TACTTGGAGG ATGTTCTGTC CAAGACGAAC TACGAGTTCC AAAAGTTCCG CGATCGTCGT 
CCCAAGCGGG ATCCGCCTGA GCGCAGGATG AAGCACGAGG CTATGATAGA ACCGTATCTG 
CGACGCATCA GGAACTCGTA CGATAGTCGT GTGCTCGACA AGCTGCGTTT GCCGGAGTCG 
GAGGGCTGCG AGGATATTGA TTTTATAGCC GATTTGGTTT ACTACATATG CGAAAACGAA 
CCAGAGGGTG CCATATTAGT GTTCCTGCCT GGTTATGATA AGATCTCCCA ACTGTACAAT 
ATTCTGGATA AACCAAAGAC TTCGAAGGGC CAAAGGTGGC GGGATCATAT GGCCGTATTT 
CCATTACATT CCCTAATGCA GTCAGGGGAA CAGCAGGCAG TGTTCAGGCG ACCACCGGCG 
GGCCAGCGTA AGGTTATAAT CTCTACAATT ATCGCCGAAA CGTCGGTGAC TATTGACGAC 
GTGGTTTATG TGATAAACTC TGGCCGGACG AAAGCCACCA ACTATGACAT CGAAACGAAC 
ATACAGTCGC TGGATGAGGT TTGGGTGACC AAAGCAAATA CCCAGCAGCG GAGAGGCAGG 
GCAGGTCGAG TGCGGCCGGG CATATGCTAT AATCTGTTCT CCCGCGCTAG AGAGGATCGC 
ATGGACGATA TTCCGACGCC GGAGATACTA CGCTCCAAGC TGGAATCCAT AATTTTAAGC 
CTCAAGCTAC TGCACATCGA CGATCCGTAT CGGTTCCTGC AAACTCTTAT TAATGCCCCC 
AATCCCGAGG CCATAAAGAT GGGAGTGGAA CTGCTAAAGC GCATTGAGGC GTTGGACCAG 
ACGGGCACCC TTACTCCACT GGGCATGCAC TTGGCAAAGC TGCCCATTGA TCCGCAGATG 
GGAAAAATGA TCCTGATGTC GGCGCTCTTC TGTTGCTTGG ATCCCATCAC TTCGGCGGCG 
GCTGCCTTGT CCTTTAAGTC CCCCTTCTAC TCGCCTTTGG GTAAAGAGAG CCGAGTGGAT 
GAAATCAAGC GGAGAATGGC CCGAAATATG CGGAGCGATC ACCTGATGGT GCACAATACC 
ATTATTGCGT ACCGCGATAG CCGCTATTCG CACGCGGAAC GAGACTTCTG CTATAAGAAT 
TTCCTCAGCT CCATGACACT GCAGCAGTTG GAGAGGATGA AGAATCAGTT CAGTGAGCTG 
CTCTACAACT ACAAATTTCT GGCATCGTCA AACTGCAAGG ATGCTGCATC CAATAAGAAT 
TCTGAGAAGA TTCCCCTACT GAGGGCCATT ATAGGAGCGG GACTCTATCC CAACATGGCT 
CACTTGCGAA AATCACGGCA GATCAAGAAT AGAGTACGCG CTATCCACAC CATGGCAACG 
GACGATGGAC GCCGCGTTAA CTTTCATCCC TCCTCGGTGA ACAGCGGAGA GAGTGGCTTC 
GACTCGGCAT ACTTTGTCTA CTTTCAGCGG CAGAAGTCCA CCGATCTTTT CCTGCTCGAC 
TCCACAATGG TTTTTCCCAT GGCACTGATC ATTTTCGGAG ATGGCGTGGA AGCGGGAGTG 
ACCCAAAATA CGCCATATCT GTGCGTGGCC AAGACATATT ACTTTAAATG CAATCGTGAA 
ACTGCCGACG TAGTCATACA ATTAAGAAGC AATTTGGAGA AACTGCTGCT CAAAAAGGCT 
TTGTATCCGG CCCCGATCGA GGAGAACGGA TATGAAAAGC AGTTGATCAA AGCCATCGAA 
TTGCTTCTCT CGCTAGACGA AAGACTCGGC GAGGACTATA TTTCATCCGA TGAAATCGAT 
GACATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Rhau cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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