Dhx36 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dhx36
Entrez Gene ID	310461
Full Name	DEAH-box helicase 36
General protein information	Preferred Names DEAH-box helicase 36 Names ATP-dependent RNA helicase DHX36 DEAH (Asp-Glu-Ala-His) box polypeptide 36 probable ATP-dependent RNA helicase DHX36
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q31

mRNA and Protein(s)

mRNA	Protein	Name
XM_008761047.2	XP_008759269.1	ATP-dependent RNA helicase DHX36 isoform X1
NM_001107678.1	NP_001101148.1	ATP-dependent DNA/RNA helicase DHX36

Pathways from BioSystems

Homologies

Homo sapiens (human)	DHX36	NP_065916.2
Arabidopsis thaliana (thale cress)	AT2G35920	NP_850255.1
Arabidopsis thaliana (thale cress)	AT1G48650	NP_175298.2
Bos taurus (cattle)	DHX36	NP_001073720.1
Rattus norvegicus (Norway rat)	Dhx36	NP_001101148.1
Drosophila melanogaster (fruit fly)	CG9323	NP_001260639.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008239	XP_317231.4
Arabidopsis thaliana (thale cress)	AT2G01130	NP_178223.2
Xenopus tropicalis (tropical clawed frog)	dhx36	XP_002933292.1
Mus musculus (house mouse)	Dhx36	NP_082412.2
Arabidopsis thaliana (thale cress)	AT5G04895	NP_680142.2
	DHX36	XP_001106252.1
Gallus gallus (chicken)	DHX36	XP_422834.3
Danio rerio (zebrafish)	si:dkey-119o24.1	NP_001122016.1
Pan troglodytes (chimpanzee)	DHX36	XP_001147599.1
Canis lupus familiaris (dog)	DHX36	XP_534311.3

Gene Ontology
Bibliography

Related articles in PubMed

The G-Quadruplex-Specific RNA Helicase DHX36 Regulates p53 Pre-mRNA 3'-End Processing Following UV-Induced DNA Damage.
Newman M, Sfaxi R, Saha A, Monchaud D, Teulade-Fichou MP, Vagner S
Journal of molecular biology429(21)3121-3131(2017 Oct)

Post-transcriptional Regulation of Nkx2-5 by RHAU in Heart Development.
Nie J, Jiang M, Zhang X, Tang H, Jin H, Huang X, Yuan B, Zhang C, Lai JC, Nagamine Y, Pan D, Wang W, Yang Z
Cell reports13(4)723-732(2015 Oct)

A G-quadruplex DNA structure resolvase, RHAU, is essential for spermatogonia differentiation.
Gao X, Ma W, Nie J, Zhang C, Zhang J, Yao G, Han J, Xu J, Hu B, Du Y, Shi Q, Yang Z, Huang X, Zhang Y
Cell death & disease6e1610(2015 Jan)

Biochemical characterization of G4 quadruplex telomerase RNA unwinding by the RNA helicase RHAU.
Booy EP, McRae EK, McKenna SA
Methods in molecular biology (Clifton, N.J.)1259125-35(2015)

The RNA helicase RHAU (DHX36) suppresses expression of the transcription factor PITX1.
Booy EP, Howard R, Marushchak O, Ariyo EO, Meier M, Novakowski SK, Deo SR, Dzananovic E, Stetefeld J, McKenna SA
Nucleic acids research42(5)3346-61(2014 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DHX36 is required for dendritic localization of pre-miR-134 in hippocampal neurons
Title: The DEAH-box helicase DHX36 mediates dendritic localization of the neuronal precursor-microRNA-134.

The following Dhx36 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dhx36 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05572	NM_001107678.1 Latest version!	Rattus norvegicus DEAH-box helicase 36 (Dhx36), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$734.30 $1049.00
ORa46845	XM_008761047.2 Latest version!	Rattus norvegicus DEAH-box helicase 36 (Dhx36), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dhx36 ( NM_001107678.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101148.1 Rattus norvegicus DEAH-box helicase 36 (Dhx36), mRNA.

Price & Availability↑

CloneID	ORa05572
Clone ID Related Accession (Same CDS sequence)	NM_001107678.1
Accession Version	NM_001107678.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3003bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Rattus norvegicus(Norway rat)
Product	ATP-dependent DNA/RNA helicase DHX36
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474003.2. On or before Oct 4, 2007 this sequence version replaced XM_001058645.1, XM_227203.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAGCTACG ACTATCATCA GAGCTGGAGC CGCGACGGGG GCCCACGGGG CTCCGGCCAG 
GGCTCTGGCG GCGGCGGTGG AGGGAGCCGG GGCTCCGGCG GCGGCGGAGG GGGCCGCGGC 
GGCCGGGGCC GGCATCCGGC CCACCTTAAG GGTCGCGAGA TAGGCCTATG GTACGCAAAA 
AAGCAAACGC AGAAGAACAA GGAGGCTGAG AGACAAGAGA GAGCTGTAGT GCACATGGAT 
GAACGGCGAG AAGAGCAAAT TGTGCAGCTG CTAAATTCAG TCCAAGCAAA GAATGATAAA 
GATTCAGAAG CACAGATATC CTGGTTTGCT CCTGAGGATC ATGGGTATGG TACTGAAGTT 
TCTTCAGAGA AAAAAATAAA CTCAGAGAAG AAACTTGACA ACCAGGAAAA GAAATTGCTG 
AACCAAGAAA AAAAGACATA TAGGATCACA GACAAATCAT ATATTGACCG AGATTCTGAG 
TATTTATTGC AACAAAATGA ACCAAACCTA GGCTTAGACC AACAATTATT GGAAGATTTA 
CAAAAGAAAA AAACTGACCC TCGGTATATA GAGATGCAGC GTTTCAGAAA AAAGCTGCCT 
TCATATGGAA TGCAGAAGGA GCTGGTAAAT TTAATCAATA ACCATCAGGT GACAGTGATA 
AGTGGTGAGA CTGGTTGTGG CAAAACCACT CAGGTTACTC AGTTCATCTT GGATAACTAC 
ATCGAAAGAG GAATAGGGTC TGCCTGCAGA ATAGTGTGTA CTCAGCCAAG AAGAATTAGT 
GCCATTTCAG TTGCTGAGAG GGTGGCTGCA GAAAGGGCAG AGTCTTGTGG CAATGGTAAT 
AGTACTGGAT ACCAGATTCG TCTCCAAAGT CGGTTGCCAA GGAAACAAGG CTCTATCCTA 
TACTGCACAA CAGGAATCAT TCTTCAGTGG CTCCAGTCAG ACTCACGTTT GTCCAGTGTT 
AGTCATATTG TACTTGATGA AATTCATGAA AGGAATCTAC AGTCAGATGT TTTAATGACT 
GTTATTAAAG ATCTCCTCCA TTTTCGATCG GATCTCAAAG TAATATTGAT GAGTGCAACC 
TTGAATGCTG AGAAATTTTC AGAATATTTT GGTAACTGTC CAATGATACA TATACCTGGG 
TTTACTTTTC CAGTTGTGGA ATATCTTTTG GAAGATATCA TTGAAAAAAT AAGATATTTT 
CCAGAACAAA AAGAACATAG ATCCCAGTTC AAAAGGGGTT TCATGCAGGG TCATGTAAAT 
AGACAAGAAA AAGAAGAAAA AGAGGCGATC TATAAAGAAC GCTGGCCAGC TTATATAAAG 
GAACTACAGA CAAGATACTC TGCAAGTACC ATAGATGTTT TGGAAATGAT GGATGATGAT 
AAAGTTGATC TGAATTTGAT TGCTGCCCTT ATTCGATACA TTGTTTTGGA AGAAGAGGAC 
GGTGCAATAT TGGTCTTTCT ACCAGGCTGG GACAATATCA GTACTTTGCA TGATCTCTTG 
ATGTCACAAG TGATGTTTAA ATCAGATAGG TTTCTTATTA TACCTTTACA TTCACTGATG 
CCCACTGTAA ACCAGACACA GGTATTTAAA AAAACTCCTC CTGGTGTTCG GAAAATAGTA 
ATTGCTACCA ACATTGCAGA GACTAGCATC ACCATAGATG ATGTGGTCTA TGTAATAGAT 
GGAGGGAAAA TTAAAGAAAC ACACTTTGAC ACACAGAACA ACATCAGTAC CATGTCTGCT 
GAGTGGGTTA GTAAAGCTAA TGCCAAACAG AGGAAAGGGA GAGCTGGAAG AGTTCAGCCT 
GGTCATTGTT ATCATCTGTA TAATGGTCTT AGAGCAAGTC TTCTAGATGA CTACCAACTA 
CCAGAAATTT TGAGAACTCC TTTGGAAGAA CTTTGTTTGC AAATAAAGAT CTTGAGGCTG 
GGTGGAATTG CTTATTTTCT GAGTAGGTTA ATGGATCCAC CATCAGATGA GGCAGTAGTG 
CTCTCCATAA AACATCTAAT GGAACTGAGT GCTTTGGATA AGCAAGAAGA ATTGACACCT 
CTTGGAGTCC ATTTAGCCCG ACTGCCTGTT GAGCCACATA TTGGAAAAAT GATTCTCTTT 
GGAGCTTTGT TCTGTTGCTT AGATCCAGTC CTCACCATTG CTGCCAGTCT CAGCTTTAAA 
GATCCCTTTG TCATTCCATT GGGAAAAGAA AAGATTGCAG ATGCAAGAAG AAAGGAACTG 
GCAAAGGAAA CTAGAAGTGA TCATCTGACA GTTGTGAATG CGTTTGAGGG CTGGGAAGAA 
GCTAAACGGC GTGGTTTCAG ATATGAAAAA GACTACTGCT GGGAATATTT TCTCTCTTCA 
AACACACTAC AGATGCTGCA TAACATGAAG GGACAGTTTG CTGAGCATCT TCTTGGAGCT 
GGATTTGTAA GCAGTAGAAG TCCCAAAGAT CCAAAAGCTA ATATAAATTC AGATAATGAG 
AAGATCATTA AAGCTGTCAT CTGTGCTGGT TTATATCCCA AAGTTGCTAA AATCCGACTA 
AATTTGGGTA AAAAAAGGAA AATGGTGAAA GTTCACACAA AGTCTGATGG TCTGGTTTCT 
ATTCATCCTA AGTCTGTCAA TGTGGAGCAG ACAGACTTCC ACTACAACTG GCTTATCTAT 
CACCTGAAGA TGAGAACAAG CAGTATATAC TTGTACGACT GCACAGAAGT CTCACCATAC 
TGCCTCTTGT TCTTTGGAGG AGATATTTCC ATCCAGAAAG ATAAGGATCA GGAAATTATT 
GCTGTAGACG AATGGATTGT GTTTCAGTCT CCAGAAAGAA TTGCTCATCT TGTTAAGGGA 
CTAAGAAAGG AACTGGATAT CCTTCTACAA GAGAAGATTG AGTGCCCTCA TCCTGTAGAT 
TGGAACGACA CCAAATCCAG AGACTGTGCA GTACTGTCAG CGATTCTAGA CTTGATCAAA 
ACACAAGAAA AGGCTATTCC AAGGAACTTG CCACCACGAT CACAGGATGG ATATTACAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101148.1
CDS	77..3079
Translation	MSYDYHQSWSRDGGPRGSGQGSGGGGGGSRGSGGGGGGRGGRGRHPAHLKGREIGLWYAKKQTQKNKEAERQERAVVHMDERREEQIVQLLNSVQAKNDKDSEAQISWFAPEDHGYGTEVSSEKKINSEKKLDNQEKKLLNQEKKTYRITDKSYIDRDSEYLLQQNEPNLGLDQQLLEDLQKKKTDPRYIEMQRFRKKLPSYGMQKELVNLINNHQVTVISGETGCGKTTQVTQFILDNYIERGIGSACRIVCTQPRRISAISVAERVAAERAESCGNGNSTGYQIRLQSRLPRKQGSILYCTTGIILQWLQSDSRLSSVSHIVLDEIHERNLQSDVLMTVIKDLLHFRSDLKVILMSATLNAEKFSEYFGNCPMIHIPGFTFPVVEYLLEDIIEKIRYFPEQKEHRSQFKRGFMQGHVNRQEKEEKEAIYKERWPAYIKELQTRYSASTIDVLEMMDDDKVDLNLIAALIRYIVLEEEDGAILVFLPGWDNISTLHDLLMSQVMFKSDRFLIIPLHSLMPTVNQTQVFKKTPPGVRKIVIATNIAETSITIDDVVYVIDGGKIKETHFDTQNNISTMSAEWVSKANAKQRKGRAGRVQPGHCYHLYNGLRASLLDDYQLPEILRTPLEELCLQIKILRLGGIAYFLSRLMDPPSDEAVVLSIKHLMELSALDKQEELTPLGVHLARLPVEPHIGKMILFGALFCCLDPVLTIAASLSFKDPFVIPLGKEKIADARRKELAKETRSDHLTVVNAFEGWEEAKRRGFRYEKDYCWEYFLSSNTLQMLHNMKGQFAEHLLGAGFVSSRSPKDPKANINSDNEKIIKAVICAGLYPKVAKIRLNLGKKRKMVKVHTKSDGLVSIHPKSVNVEQTDFHYNWLIYHLKMRTSSIYLYDCTEVSPYCLLFFGGDISIQKDKDQEIIAVDEWIVFQSPERIAHLVKGLRKELDILLQEKIECPHPVDWNDTKSRDCAVLSAILDLIKTQEKAIPRNLPPRSQDGYYS

Target ORF information:

RefSeq Version	NM_001107678.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEAH-box helicase 36 (Dhx36), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107678.1

ORF Insert Sequence:

ATGAGCTACG ACTATCATCA GAGCTGGAGC CGCGACGGGG GCCCACGGGG CTCCGGCCAG 
GGCTCTGGCG GCGGCGGTGG AGGGAGCCGG GGCTCCGGCG GCGGCGGAGG GGGCCGCGGC 
GGCCGGGGCC GGCATCCGGC CCACCTTAAG GGTCGCGAGA TAGGCCTATG GTACGCAAAA 
AAGCAAACGC AGAAGAACAA GGAGGCTGAG AGACAAGAGA GAGCTGTAGT GCACATGGAT 
GAACGGCGAG AAGAGCAAAT TGTGCAGCTG CTAAATTCAG TCCAAGCAAA GAATGATAAA 
GATTCAGAAG CACAGATATC CTGGTTTGCT CCTGAGGATC ATGGGTATGG TACTGAAGTT 
TCTTCAGAGA AAAAAATAAA CTCAGAGAAG AAACTTGACA ACCAGGAAAA GAAATTGCTG 
AACCAAGAAA AAAAGACATA TAGGATCACA GACAAATCAT ATATTGACCG AGATTCTGAG 
TATTTATTGC AACAAAATGA ACCAAACCTA GGCTTAGACC AACAATTATT GGAAGATTTA 
CAAAAGAAAA AAACTGACCC TCGGTATATA GAGATGCAGC GTTTCAGAAA AAAGCTGCCT 
TCATATGGAA TGCAGAAGGA GCTGGTAAAT TTAATCAATA ACCATCAGGT GACAGTGATA 
AGTGGTGAGA CTGGTTGTGG CAAAACCACT CAGGTTACTC AGTTCATCTT GGATAACTAC 
ATCGAAAGAG GAATAGGGTC TGCCTGCAGA ATAGTGTGTA CTCAGCCAAG AAGAATTAGT 
GCCATTTCAG TTGCTGAGAG GGTGGCTGCA GAAAGGGCAG AGTCTTGTGG CAATGGTAAT 
AGTACTGGAT ACCAGATTCG TCTCCAAAGT CGGTTGCCAA GGAAACAAGG CTCTATCCTA 
TACTGCACAA CAGGAATCAT TCTTCAGTGG CTCCAGTCAG ACTCACGTTT GTCCAGTGTT 
AGTCATATTG TACTTGATGA AATTCATGAA AGGAATCTAC AGTCAGATGT TTTAATGACT 
GTTATTAAAG ATCTCCTCCA TTTTCGATCG GATCTCAAAG TAATATTGAT GAGTGCAACC 
TTGAATGCTG AGAAATTTTC AGAATATTTT GGTAACTGTC CAATGATACA TATACCTGGG 
TTTACTTTTC CAGTTGTGGA ATATCTTTTG GAAGATATCA TTGAAAAAAT AAGATATTTT 
CCAGAACAAA AAGAACATAG ATCCCAGTTC AAAAGGGGTT TCATGCAGGG TCATGTAAAT 
AGACAAGAAA AAGAAGAAAA AGAGGCGATC TATAAAGAAC GCTGGCCAGC TTATATAAAG 
GAACTACAGA CAAGATACTC TGCAAGTACC ATAGATGTTT TGGAAATGAT GGATGATGAT 
AAAGTTGATC TGAATTTGAT TGCTGCCCTT ATTCGATACA TTGTTTTGGA AGAAGAGGAC 
GGTGCAATAT TGGTCTTTCT ACCAGGCTGG GACAATATCA GTACTTTGCA TGATCTCTTG 
ATGTCACAAG TGATGTTTAA ATCAGATAGG TTTCTTATTA TACCTTTACA TTCACTGATG 
CCCACTGTAA ACCAGACACA GGTATTTAAA AAAACTCCTC CTGGTGTTCG GAAAATAGTA 
ATTGCTACCA ACATTGCAGA GACTAGCATC ACCATAGATG ATGTGGTCTA TGTAATAGAT 
GGAGGGAAAA TTAAAGAAAC ACACTTTGAC ACACAGAACA ACATCAGTAC CATGTCTGCT 
GAGTGGGTTA GTAAAGCTAA TGCCAAACAG AGGAAAGGGA GAGCTGGAAG AGTTCAGCCT 
GGTCATTGTT ATCATCTGTA TAATGGTCTT AGAGCAAGTC TTCTAGATGA CTACCAACTA 
CCAGAAATTT TGAGAACTCC TTTGGAAGAA CTTTGTTTGC AAATAAAGAT CTTGAGGCTG 
GGTGGAATTG CTTATTTTCT GAGTAGGTTA ATGGATCCAC CATCAGATGA GGCAGTAGTG 
CTCTCCATAA AACATCTAAT GGAACTGAGT GCTTTGGATA AGCAAGAAGA ATTGACACCT 
CTTGGAGTCC ATTTAGCCCG ACTGCCTGTT GAGCCACATA TTGGAAAAAT GATTCTCTTT 
GGAGCTTTGT TCTGTTGCTT AGATCCAGTC CTCACCATTG CTGCCAGTCT CAGCTTTAAA 
GATCCCTTTG TCATTCCATT GGGAAAAGAA AAGATTGCAG ATGCAAGAAG AAAGGAACTG 
GCAAAGGAAA CTAGAAGTGA TCATCTGACA GTTGTGAATG CGTTTGAGGG CTGGGAAGAA 
GCTAAACGGC GTGGTTTCAG ATATGAAAAA GACTACTGCT GGGAATATTT TCTCTCTTCA 
AACACACTAC AGATGCTGCA TAACATGAAG GGACAGTTTG CTGAGCATCT TCTTGGAGCT 
GGATTTGTAA GCAGTAGAAG TCCCAAAGAT CCAAAAGCTA ATATAAATTC AGATAATGAG 
AAGATCATTA AAGCTGTCAT CTGTGCTGGT TTATATCCCA AAGTTGCTAA AATCCGACTA 
AATTTGGGTA AAAAAAGGAA AATGGTGAAA GTTCACACAA AGTCTGATGG TCTGGTTTCT 
ATTCATCCTA AGTCTGTCAA TGTGGAGCAG ACAGACTTCC ACTACAACTG GCTTATCTAT 
CACCTGAAGA TGAGAACAAG CAGTATATAC TTGTACGACT GCACAGAAGT CTCACCATAC 
TGCCTCTTGT TCTTTGGAGG AGATATTTCC ATCCAGAAAG ATAAGGATCA GGAAATTATT 
GCTGTAGACG AATGGATTGT GTTTCAGTCT CCAGAAAGAA TTGCTCATCT TGTTAAGGGA 
CTAAGAAAGG AACTGGATAT CCTTCTACAA GAGAAGATTG AGTGCCCTCA TCCTGTAGAT 
TGGAACGACA CCAAATCCAG AGACTGTGCA GTACTGTCAG CGATTCTAGA CTTGATCAAA 
ACACAAGAAA AGGCTATTCC AAGGAACTTG CCACCACGAT CACAGGATGG ATATTACAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dhx36 ( XM_008761047.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759269.1 Rattus norvegicus DEAH-box helicase 36 (Dhx36), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa46845
Clone ID Related Accession (Same CDS sequence)	XM_008761047.2
Accession Version	XM_008761047.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ATP-dependent RNA helicase DHX36 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761047.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTACG ACTATCATCA GAGCTGGAGC CGCGACGGGG GCCCACGGGG CTCCGGCCAG 
GGCTCTGGCG GCGGCGGTGG AGGGAGCCGG GGCTCCGGCG GCGGCGGAGG GGGCCGCGGC 
GGCCGGGGCC GGCATCCGGC CCACCTTAAG GGTCGCGAGA TAGGCCTATG GTACGCAAAA 
AAGCAAACGC AGAAGAACAA GGAGGCTGAG AGACAAGAGA GAGCTGTAGT GCACATGGAT 
GAACGGCGAG AAGAGCAAAT TGTGCAGCTG CTAAATTCAG TCCAAGCAAA GAATGATAAA 
GATTCAGAAG CACAGATATC CTGGTTTGCT CCTGAGGATC ATGGGTATGG TACTGAAGTT 
TCTTCAGAGA AAAAAATAAA CTCAGAGAAG AAACTTGACA ACCAGGAAAA GAAATTGCTG 
AACCAAGAAA AAAAGACATA TAGGATCACA GACAAATCAT ATATTGACCG AGATTCTGAG 
TATTTATTGC AACAAAATGA ACCAAACCTA GGCTTAGACC AACAATTATT GGAAGATTTA 
CAAAAGAAAA AAACTGACCC TCGGTATATA GAGATGCAGC GTTTCAGAAA AAAGCTGCCT 
TCATATGGAA TGCAGAAGGA GCTGGTAAAT TTAATCAATA ACCATCAGGT GACAGTGATA 
AGTGGTGAGA CTGGTTGTGG CAAAACCACT CAGGTTACTC AGTTCATCTT GGATAACTAC 
ATCGAAAGAG GAATAGGGTC TGCCTGCAGA ATAGTGTGTA CTCAGCCAAG AAGAATTAGT 
GCCATTTCAG TTGCTGAGAG GGTGGCTGCA GAAAGGGCAG AGTCTTGTGG CAATGGTAAT 
AGTACTGGAT ACCAGATTCG TCTCCAAAGT CGGTTGCCAA GGAAACAAGG CTCTATCCTA 
TACTGCACAA CAGGAATCAT TCTTCAGTGG CTCCAGTCAG ACTCACGTTT GTCCAGTGTT 
AGTCATATTG TACTTGATGA AATTCATGAA AGGAATCTAC AGTCAGATGT TTTAATGACT 
GTTATTAAAG ATCTCCTCCA TTTTCGATCG GATCTCAAAG TAATATTGAT GAGTGCAACC 
TTGAATGCTG AGAAATTTTC AGAATATTTT GGTAACTGTC CAATGATACA TATACCTGGG 
TTTACTTTTC CAGTTGTGGA ATATCTTTTG GAAGATATCA TTGAAAAAAT AAGATATTTT 
CCAGAACAAA AAGAACATAG ATCCCAGTTC AAAAGGGGTT TCATGCAGGG TCATGTAAAT 
AGACAAGAAA AAGAAGAAAA AGAGGCGATC TATAAAGAAC GCTGGCCAGC TTATATAAAG 
GAACTACAGA CAAGATACTC TGCAAGTACC ATAGATGTTT TGGAAATGAT GGATGATGAT 
AAAGTTGATC TGAATTTGAT TGCTGCCCTT ATTCGATACA TTGTTTTGGA AGAAGAGGAC 
GGTGCAATAT TGGTCTTTCT ACCAGGCTGG GACAATATCA GTACTTTGCA TGATCTCTTG 
ATGTCACAAG TGATGTTTAA ATCAGATAGG TTTCTTATTA TACCTTTACA TTCACTGATG 
CCCACTGTAA ACCAGACACA GGTATTTAAA AAAACTCCTC CTGGTGTTCG GAAAATAGTA 
ATTGCTACCA ACATTGCAGA GACTAGCATC ACCATAGATG ATGTGGTCTA TGTAATAGAT 
GGAGGGAAAA TTAAAGAAAC ACACTTTGAC ACACAGAACA ACATCAGTAC CATGTCTGCT 
GAGTGGGTTA GTAAAGCTAA TGCCAAACAG AGGAAAGGGA GAGCTGGAAG AGTTCAGCCT 
GGTCATTGTT ATCATCTGTA TAATGGTCTT AGAGCAAGTC TTCTAGATGA CTACCAACTA 
CCAGAAATTT TGAGAACTCC TTTGGAAGAA CTTTGTTTGC AAATAAAGAT CTTGAGGCTG 
GGTGGAATTG CTTATTTTCT GAGTAGGTTA ATGGATCCAC CATCAGATGA GGCAGTAGTG 
CTCTCCATAA AACATCTAAT GGAACTGAGT GCTTTGGATA AGCAAGAAGA ATTGACACCT 
CTTGGAGTCC ATTTAGCCCG ACTGCCTGTT GAGCCACATA TTGGAAAAAT GATTCTCTTT 
GGAGCTTTGT TCTGTTGCTT AGATCCAGTC CTCACCATTG CTGCCAGTCT CAGCTTTAAA 
GATCCCTTTG TCATTCCATT GGGAAAAGAA AAGATTGCAG ATGCAAGAAG AAAGGAACTG 
GCAAAGGAAA CTAGAAGTGA TCATCTGACA GTTGTGAATG CGTTTGAGGG CTGGGAAGAA 
GCTAAACGGC GTGGTTTCAG ATATGAAAAA GACTACTGCT GGGAATATTT TCTCTCTTCA 
AACACACTAC AGATAATGAG AAGATCATTA AAGCTGTCAT CTGTGCTGGT TTATATCCCA 
AAGTTGCTAA AATCCGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759269.1
CDS	79..2499
Translation	MSYDYHQSWSRDGGPRGSGQGSGGGGGGSRGSGGGGGGRGGRGRHPAHLKGREIGLWYAKKQTQKNKEAERQERAVVHMDERREEQIVQLLNSVQAKNDKDSEAQISWFAPEDHGYGTEVSSEKKINSEKKLDNQEKKLLNQEKKTYRITDKSYIDRDSEYLLQQNEPNLGLDQQLLEDLQKKKTDPRYIEMQRFRKKLPSYGMQKELVNLINNHQVTVISGETGCGKTTQVTQFILDNYIERGIGSACRIVCTQPRRISAISVAERVAAERAESCGNGNSTGYQIRLQSRLPRKQGSILYCTTGIILQWLQSDSRLSSVSHIVLDEIHERNLQSDVLMTVIKDLLHFRSDLKVILMSATLNAEKFSEYFGNCPMIHIPGFTFPVVEYLLEDIIEKIRYFPEQKEHRSQFKRGFMQGHVNRQEKEEKEAIYKERWPAYIKELQTRYSASTIDVLEMMDDDKVDLNLIAALIRYIVLEEEDGAILVFLPGWDNISTLHDLLMSQVMFKSDRFLIIPLHSLMPTVNQTQVFKKTPPGVRKIVIATNIAETSITIDDVVYVIDGGKIKETHFDTQNNISTMSAEWVSKANAKQRKGRAGRVQPGHCYHLYNGLRASLLDDYQLPEILRTPLEELCLQIKILRLGGIAYFLSRLMDPPSDEAVVLSIKHLMELSALDKQEELTPLGVHLARLPVEPHIGKMILFGALFCCLDPVLTIAASLSFKDPFVIPLGKEKIADARRKELAKETRSDHLTVVNAFEGWEEAKRRGFRYEKDYCWEYFLSSNTLQIMRRSLKLSSVLVYIPKLLKSD

Target ORF information:

RefSeq Version	XM_008761047.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEAH-box helicase 36 (Dhx36), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761047.2

ORF Insert Sequence:

ATGAGCTACG ACTATCATCA GAGCTGGAGC CGCGACGGGG GCCCACGGGG CTCCGGCCAG 
GGCTCTGGCG GCGGCGGTGG AGGGAGCCGG GGCTCCGGCG GCGGCGGAGG GGGCCGCGGC 
GGCCGGGGCC GGCATCCGGC CCACCTTAAG GGTCGCGAGA TAGGCCTATG GTACGCAAAA 
AAGCAAACGC AGAAGAACAA GGAGGCTGAG AGACAAGAGA GAGCTGTAGT GCACATGGAT 
GAACGGCGAG AAGAGCAAAT TGTGCAGCTG CTAAATTCAG TCCAAGCAAA GAATGATAAA 
GATTCAGAAG CACAGATATC CTGGTTTGCT CCTGAGGATC ATGGGTATGG TACTGAAGTT 
TCTTCAGAGA AAAAAATAAA CTCAGAGAAG AAACTTGACA ACCAGGAAAA GAAATTGCTG 
AACCAAGAAA AAAAGACATA TAGGATCACA GACAAATCAT ATATTGACCG AGATTCTGAG 
TATTTATTGC AACAAAATGA ACCAAACCTA GGCTTAGACC AACAATTATT GGAAGATTTA 
CAAAAGAAAA AAACTGACCC TCGGTATATA GAGATGCAGC GTTTCAGAAA AAAGCTGCCT 
TCATATGGAA TGCAGAAGGA GCTGGTAAAT TTAATCAATA ACCATCAGGT GACAGTGATA 
AGTGGTGAGA CTGGTTGTGG CAAAACCACT CAGGTTACTC AGTTCATCTT GGATAACTAC 
ATCGAAAGAG GAATAGGGTC TGCCTGCAGA ATAGTGTGTA CTCAGCCAAG AAGAATTAGT 
GCCATTTCAG TTGCTGAGAG GGTGGCTGCA GAAAGGGCAG AGTCTTGTGG CAATGGTAAT 
AGTACTGGAT ACCAGATTCG TCTCCAAAGT CGGTTGCCAA GGAAACAAGG CTCTATCCTA 
TACTGCACAA CAGGAATCAT TCTTCAGTGG CTCCAGTCAG ACTCACGTTT GTCCAGTGTT 
AGTCATATTG TACTTGATGA AATTCATGAA AGGAATCTAC AGTCAGATGT TTTAATGACT 
GTTATTAAAG ATCTCCTCCA TTTTCGATCG GATCTCAAAG TAATATTGAT GAGTGCAACC 
TTGAATGCTG AGAAATTTTC AGAATATTTT GGTAACTGTC CAATGATACA TATACCTGGG 
TTTACTTTTC CAGTTGTGGA ATATCTTTTG GAAGATATCA TTGAAAAAAT AAGATATTTT 
CCAGAACAAA AAGAACATAG ATCCCAGTTC AAAAGGGGTT TCATGCAGGG TCATGTAAAT 
AGACAAGAAA AAGAAGAAAA AGAGGCGATC TATAAAGAAC GCTGGCCAGC TTATATAAAG 
GAACTACAGA CAAGATACTC TGCAAGTACC ATAGATGTTT TGGAAATGAT GGATGATGAT 
AAAGTTGATC TGAATTTGAT TGCTGCCCTT ATTCGATACA TTGTTTTGGA AGAAGAGGAC 
GGTGCAATAT TGGTCTTTCT ACCAGGCTGG GACAATATCA GTACTTTGCA TGATCTCTTG 
ATGTCACAAG TGATGTTTAA ATCAGATAGG TTTCTTATTA TACCTTTACA TTCACTGATG 
CCCACTGTAA ACCAGACACA GGTATTTAAA AAAACTCCTC CTGGTGTTCG GAAAATAGTA 
ATTGCTACCA ACATTGCAGA GACTAGCATC ACCATAGATG ATGTGGTCTA TGTAATAGAT 
GGAGGGAAAA TTAAAGAAAC ACACTTTGAC ACACAGAACA ACATCAGTAC CATGTCTGCT 
GAGTGGGTTA GTAAAGCTAA TGCCAAACAG AGGAAAGGGA GAGCTGGAAG AGTTCAGCCT 
GGTCATTGTT ATCATCTGTA TAATGGTCTT AGAGCAAGTC TTCTAGATGA CTACCAACTA 
CCAGAAATTT TGAGAACTCC TTTGGAAGAA CTTTGTTTGC AAATAAAGAT CTTGAGGCTG 
GGTGGAATTG CTTATTTTCT GAGTAGGTTA ATGGATCCAC CATCAGATGA GGCAGTAGTG 
CTCTCCATAA AACATCTAAT GGAACTGAGT GCTTTGGATA AGCAAGAAGA ATTGACACCT 
CTTGGAGTCC ATTTAGCCCG ACTGCCTGTT GAGCCACATA TTGGAAAAAT GATTCTCTTT 
GGAGCTTTGT TCTGTTGCTT AGATCCAGTC CTCACCATTG CTGCCAGTCT CAGCTTTAAA 
GATCCCTTTG TCATTCCATT GGGAAAAGAA AAGATTGCAG ATGCAAGAAG AAAGGAACTG 
GCAAAGGAAA CTAGAAGTGA TCATCTGACA GTTGTGAATG CGTTTGAGGG CTGGGAAGAA 
GCTAAACGGC GTGGTTTCAG ATATGAAAAA GACTACTGCT GGGAATATTT TCTCTCTTCA 
AACACACTAC AGATAATGAG AAGATCATTA AAGCTGTCAT CTGTGCTGGT TTATATCCCA 
AAGTTGCTAA AATCCGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The G-Quadruplex-Specific RNA Helicase DHX36 Regulates p53 Pre-mRNA 3'-End Processing Following UV-Induced DNA Damage.
Journal of molecular biology429(21)3121-3131(2017 Oct)
Newman M,Sfaxi R,Saha A,Monchaud D,Teulade-Fichou MP,Vagner S

Post-transcriptional Regulation of Nkx2-5 by RHAU in Heart Development.
Cell reports13(4)723-732(2015 Oct)
Nie J,Jiang M,Zhang X,Tang H,Jin H,Huang X,Yuan B,Zhang C,Lai JC,Nagamine Y,Pan D,Wang W,Yang Z

A G-quadruplex DNA structure resolvase, RHAU, is essential for spermatogonia differentiation.
Cell death & disease6e1610(2015 Jan)
Gao X,Ma W,Nie J,Zhang C,Zhang J,Yao G,Han J,Xu J,Hu B,Du Y,Shi Q,Yang Z,Huang X,Zhang Y

Biochemical characterization of G4 quadruplex telomerase RNA unwinding by the RNA helicase RHAU.
Methods in molecular biology (Clifton, N.J.)1259125-35(2015)
Booy EP,McRae EK,McKenna SA

The RNA helicase RHAU (DHX36) suppresses expression of the transcription factor PITX1.
Nucleic acids research42(5)3346-61(2014 Mar)
Booy EP,Howard R,Marushchak O,Ariyo EO,Meier M,Novakowski SK,Deo SR,Dzananovic E,Stetefeld J,McKenna SA

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Dhx36 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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