Cen cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cen
Entrez Gene ID	35330
Full Name	Centrocortin
Synonyms	38E.1,CG1962,Dmel\CG1962,cen
General protein information	Preferred Names Centrocortin Names CG1962 gene product from transcript CG1962-RA CG1962-PA CG1962-PB Cen-PA Cen-PB centrocortin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 38D5-38D5

mRNA and Protein(s)

mRNA	Protein	Name
NM_136205.4	NP_610049.1	centrocortin, isoform A
NM_165338.3	NP_724279.1	centrocortin, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Cen	NP_610049.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000264	XP_310854.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cen gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cen cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa15049	NM_136205.4 Latest version!	Drosophila melanogaster centrocortin, transcript variant A (Cen), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa15049	NM_165338.3 Latest version!	Drosophila melanogaster centrocortin, transcript variant B (Cen), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cen ( NM_136205.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610049.1 Drosophila melanogaster centrocortin, transcript variant A (Cen), mRNA.

Price & Availability↑

CloneID	OFa15049
Clone ID Related Accession (Same CDS sequence)	NM_136205.4 , NM_165338.3
Accession Version	NM_136205.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	centrocortin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_136205.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGAAT CCAATCACGG TTCGGCTGGC TGTGAAAACG TATCGCAGTT CATGCTCGAT 
GACCTACAAT TGGCAGCAGA GCTGGGAAAA ACGCTCCTGG AACGCAATAA GGAGCTGGAA 
ACCTTCATCA AGGAGTACAA GATTAAGGGG GATGAGCAGG AACTGGAGAT CTTGCATCTG 
CGCAAGCACA TTAATGCGAT GACCGAGGTG AATGATTCAC GGCTTAAGGT CTACGAGCAG 
CTGGAAGTGG GCATTCAGGA TCTGGAGCGT GCCAATCAGC GGCTAAATCT GGAGAAGAAT 
CGGGACAAGA AGCAGATTAA GACGCTGACC ACCAACACGG AAGTCCTGGA AGCCCGCTGC 
GAGGAACTGA GTCAACTTCT GAGCGATGCT CGGCAATCCC TGAGCACCGA GCGGCGGAAG 
GTGGATCAGT ACCAGCAGGA ACGCTATAGG ATGCAGCATT CGACCGAGGG TTCTGTGTCC 
AGTCACAGCA TTCAATCGCT GTGCAAGGAG CAGAGTGTTG AGTTCTCCAA ACTAGATGTT 
ATGGCCATAG CGAACTCGAC AGGATTGGAG GATATCTCCT TCAGTAATGC CACCATGTGC 
GAGAGGACCG CCGTTAAGGG TGAAGACAAC GAGGAATTGG TAAAGCTGCT CAGCGAAATG 
GAGGTGTTAA AACGTGACTT CTTGGCCGAA CAGCAGCGGT GCACCGAGCT GGAGGAGCAG 
TTGGTGACCA TAATCCAAGA CAACCAAGGT CTGCAGACAC GTCTGCTGGA GAACAGTGCC 
AATGAGGGAA CAATGTCGAT GCACGAGGAG TTCAGCCTCC TGGACGACGT GAGACAAGGC 
CAAATGTGTA GTCGCTGCCT GAGGGATATT AACGAAAGCA ACACCAATAT GGATGATCAA 
TCCTCCATTG CCCCAACCGA AGAAATCTAC GAAGATGACG ATCGCAGCAT ACTAAGCGAA 
AGCACCTCGA AATGCGATAA TAGTGGTGCG GATTATAAGG AACGCTTCCG GATTCCCGAG 
GACCTCAATC CCAACTCTAG CGACAAACCA AATCCTTATC GGGACCTAGT CGAGAAATAT 
GAAGCTCTGG TGGAGGTGAA GAGAACCTCG AATGCGGTCA AGAGCAACTT CACCAGCAAT 
CCAGATGGGA AGACCATGAC CGAGTCCAGT CAAGGCAAAA AATCAGAAAC AATTGTTAAC 
AGCTCAAAGG AATCTGACCT TATGTTGGAT TCAACACGTA AGCGCACTCC AACCGAGTTT 
TCGGAATCAG AAACTACATC GTCGGGATTC TCGGATGAAA CTAGCAACAA GTCTACGCAA 
ACTGACGAGC GACCGAGTTA CTTCCTTTGC TCAATAAGTA ATGGAAACGA TTGTAAATTC 
AGTATTTATG ACGACGTCAG CCCCATTGAA TCTCATTTCC GTAATCGCCC GGAGTACAGG 
GAACTCTTCA AGGAGATATT TGGAGTGCTC AAGAAGGCAG CTGATAATAA CGAAGAAGAC 
AAGCTTCCGT CCCTGCATGA CGACGCACAG ATTACAGAAA AATTACCCTT GGTGGCGGCC 
AAAGTACCCC CGGTAACACC CGAGAGGGAG GAATCTCCAG ATGACTTCAT TGACGACACA 
CAGAGCGTCG TATCCTCAGT TATATCCAAC CAGTCCATTG CAATGTCCGA GTGCGTTACC 
AAACTGGAGC GGAAGACAGC CAAAAAACAC ATCTTTGATG TCCGAAACCA ACAAAACCAA 
TCGTCCCTAA CGCAATCCAC TTTGTTAAAC AGCTCCTCGA AGGAAACAAC TGTCGGCGAA 
CCGAATTATA AACCCATAAG GGAGAATGGC CAGATACTTA CTCCACTCAA GCGCGAACCA 
CTCGAATATC TAACCGTAGG TGTCGGAATC AAGAAGAAGA ACCGACGAAA GCACCGAAAC 
CAAAGCTCAT CTGGAGATCG CATCGAATTG TTTAACTCCC GGGAATTTAC TCCCAGGAAC 
AGTCCTCTGG CCATGAACAA CCGTGGTGGT GGACAAGGGA GCTCAAAGAT GTGTACGGAT 
ACATTGAATG CTGAATTCGG ACGCAGTAAC CGCAGACGGA CAACTCCATC CTCAAGCAAC 
TGGAATGGCT CTCCCATGGT GATCTATAAC AAGAATATGA ATACTCCCCA AACTTCTCGG 
GGCCGAGTAA TAGAGCTCAA TGGCGTCGAG TTTTATCATA ACACGGTATC GCAAGACTTA 
CATAAACTTA AAAAGTTAGA CTTATCCTAC GCCGAAGTTT TGCGCCGGGC CGATGCCGGC 
GAACATGGAC CCACAAGATC ACATTCCCAG CGACAGCAAC ACAACGGAGC CAACATTCGC 
AAGAGTCATC ATCATCAGTT TCGTCAAAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610049.1
CDS	270..2642
Translation	MEESNHGSAGCENVSQFMLDDLQLAAELGKTLLERNKELETFIKEYKIKGDEQELEILHLRKHINAMTEVNDSRLKVYEQLEVGIQDLERANQRLNLEKNRDKKQIKTLTTNTEVLEARCEELSQLLSDARQSLSTERRKVDQYQQERYRMQHSTEGSVSSHSIQSLCKEQSVEFSKLDVMAIANSTGLEDISFSNATMCERTAVKGEDNEELVKLLSEMEVLKRDFLAEQQRCTELEEQLVTIIQDNQGLQTRLLENSANEGTMSMHEEFSLLDDVRQGQMCSRCLRDINESNTNMDDQSSIAPTEEIYEDDDRSILSESTSKCDNSGADYKERFRIPEDLNPNSSDKPNPYRDLVEKYEALVEVKRTSNAVKSNFTSNPDGKTMTESSQGKKSETIVNSSKESDLMLDSTRKRTPTEFSESETTSSGFSDETSNKSTQTDERPSYFLCSISNGNDCKFSIYDDVSPIESHFRNRPEYRELFKEIFGVLKKAADNNEEDKLPSLHDDAQITEKLPLVAAKVPPVTPEREESPDDFIDDTQSVVSSVISNQSIAMSECVTKLERKTAKKHIFDVRNQQNQSSLTQSTLLNSSSKETTVGEPNYKPIRENGQILTPLKREPLEYLTVGVGIKKKNRRKHRNQSSSGDRIELFNSREFTPRNSPLAMNNRGGGQGSSKMCTDTLNAEFGRSNRRRTTPSSSNWNGSPMVIYNKNMNTPQTSRGRVIELNGVEFYHNTVSQDLHKLKKLDLSYAEVLRRADAGEHGPTRSHSQRQQHNGANIRKSHHHQFRQK

Target ORF information:

RefSeq Version	NM_136205.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster centrocortin, transcript variant A (Cen), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136205.4

ORF Insert Sequence:

ATGGAGGAAT CCAATCACGG TTCGGCTGGC TGTGAAAACG TATCGCAGTT CATGCTCGAT 
GACCTACAAT TGGCAGCAGA GCTGGGAAAA ACGCTCCTGG AACGCAATAA GGAGCTGGAA 
ACCTTCATCA AGGAGTACAA GATTAAGGGG GATGAGCAGG AACTGGAGAT CTTGCATCTG 
CGCAAGCACA TTAATGCGAT GACCGAGGTG AATGATTCAC GGCTTAAGGT CTACGAGCAG 
CTGGAAGTGG GCATTCAGGA TCTGGAGCGT GCCAATCAGC GGCTAAATCT GGAGAAGAAT 
CGGGACAAGA AGCAGATTAA GACGCTGACC ACCAACACGG AAGTCCTGGA AGCCCGCTGC 
GAGGAACTGA GTCAACTTCT GAGCGATGCT CGGCAATCCC TGAGCACCGA GCGGCGGAAG 
GTGGATCAGT ACCAGCAGGA ACGCTATAGG ATGCAGCATT CGACCGAGGG TTCTGTGTCC 
AGTCACAGCA TTCAATCGCT GTGCAAGGAG CAGAGTGTTG AGTTCTCCAA ACTAGATGTT 
ATGGCCATAG CGAACTCGAC AGGATTGGAG GATATCTCCT TCAGTAATGC CACCATGTGC 
GAGAGGACCG CCGTTAAGGG TGAAGACAAC GAGGAATTGG TAAAGCTGCT CAGCGAAATG 
GAGGTGTTAA AACGTGACTT CTTGGCCGAA CAGCAGCGGT GCACCGAGCT GGAGGAGCAG 
TTGGTGACCA TAATCCAAGA CAACCAAGGT CTGCAGACAC GTCTGCTGGA GAACAGTGCC 
AATGAGGGAA CAATGTCGAT GCACGAGGAG TTCAGCCTCC TGGACGACGT GAGACAAGGC 
CAAATGTGTA GTCGCTGCCT GAGGGATATT AACGAAAGCA ACACCAATAT GGATGATCAA 
TCCTCCATTG CCCCAACCGA AGAAATCTAC GAAGATGACG ATCGCAGCAT ACTAAGCGAA 
AGCACCTCGA AATGCGATAA TAGTGGTGCG GATTATAAGG AACGCTTCCG GATTCCCGAG 
GACCTCAATC CCAACTCTAG CGACAAACCA AATCCTTATC GGGACCTAGT CGAGAAATAT 
GAAGCTCTGG TGGAGGTGAA GAGAACCTCG AATGCGGTCA AGAGCAACTT CACCAGCAAT 
CCAGATGGGA AGACCATGAC CGAGTCCAGT CAAGGCAAAA AATCAGAAAC AATTGTTAAC 
AGCTCAAAGG AATCTGACCT TATGTTGGAT TCAACACGTA AGCGCACTCC AACCGAGTTT 
TCGGAATCAG AAACTACATC GTCGGGATTC TCGGATGAAA CTAGCAACAA GTCTACGCAA 
ACTGACGAGC GACCGAGTTA CTTCCTTTGC TCAATAAGTA ATGGAAACGA TTGTAAATTC 
AGTATTTATG ACGACGTCAG CCCCATTGAA TCTCATTTCC GTAATCGCCC GGAGTACAGG 
GAACTCTTCA AGGAGATATT TGGAGTGCTC AAGAAGGCAG CTGATAATAA CGAAGAAGAC 
AAGCTTCCGT CCCTGCATGA CGACGCACAG ATTACAGAAA AATTACCCTT GGTGGCGGCC 
AAAGTACCCC CGGTAACACC CGAGAGGGAG GAATCTCCAG ATGACTTCAT TGACGACACA 
CAGAGCGTCG TATCCTCAGT TATATCCAAC CAGTCCATTG CAATGTCCGA GTGCGTTACC 
AAACTGGAGC GGAAGACAGC CAAAAAACAC ATCTTTGATG TCCGAAACCA ACAAAACCAA 
TCGTCCCTAA CGCAATCCAC TTTGTTAAAC AGCTCCTCGA AGGAAACAAC TGTCGGCGAA 
CCGAATTATA AACCCATAAG GGAGAATGGC CAGATACTTA CTCCACTCAA GCGCGAACCA 
CTCGAATATC TAACCGTAGG TGTCGGAATC AAGAAGAAGA ACCGACGAAA GCACCGAAAC 
CAAAGCTCAT CTGGAGATCG CATCGAATTG TTTAACTCCC GGGAATTTAC TCCCAGGAAC 
AGTCCTCTGG CCATGAACAA CCGTGGTGGT GGACAAGGGA GCTCAAAGAT GTGTACGGAT 
ACATTGAATG CTGAATTCGG ACGCAGTAAC CGCAGACGGA CAACTCCATC CTCAAGCAAC 
TGGAATGGCT CTCCCATGGT GATCTATAAC AAGAATATGA ATACTCCCCA AACTTCTCGG 
GGCCGAGTAA TAGAGCTCAA TGGCGTCGAG TTTTATCATA ACACGGTATC GCAAGACTTA 
CATAAACTTA AAAAGTTAGA CTTATCCTAC GCCGAAGTTT TGCGCCGGGC CGATGCCGGC 
GAACATGGAC CCACAAGATC ACATTCCCAG CGACAGCAAC ACAACGGAGC CAACATTCGC 
AAGAGTCATC ATCATCAGTT TCGTCAAAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cen ( NM_165338.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724279.1 Drosophila melanogaster centrocortin, transcript variant B (Cen), mRNA.

Price & Availability↑

CloneID	OFa15049
Clone ID Related Accession (Same CDS sequence)	NM_136205.4 , NM_165338.3
Accession Version	NM_165338.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	centrocortin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165338.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGAAT CCAATCACGG TTCGGCTGGC TGTGAAAACG TATCGCAGTT CATGCTCGAT 
GACCTACAAT TGGCAGCAGA GCTGGGAAAA ACGCTCCTGG AACGCAATAA GGAGCTGGAA 
ACCTTCATCA AGGAGTACAA GATTAAGGGG GATGAGCAGG AACTGGAGAT CTTGCATCTG 
CGCAAGCACA TTAATGCGAT GACCGAGGTG AATGATTCAC GGCTTAAGGT CTACGAGCAG 
CTGGAAGTGG GCATTCAGGA TCTGGAGCGT GCCAATCAGC GGCTAAATCT GGAGAAGAAT 
CGGGACAAGA AGCAGATTAA GACGCTGACC ACCAACACGG AAGTCCTGGA AGCCCGCTGC 
GAGGAACTGA GTCAACTTCT GAGCGATGCT CGGCAATCCC TGAGCACCGA GCGGCGGAAG 
GTGGATCAGT ACCAGCAGGA ACGCTATAGG ATGCAGCATT CGACCGAGGG TTCTGTGTCC 
AGTCACAGCA TTCAATCGCT GTGCAAGGAG CAGAGTGTTG AGTTCTCCAA ACTAGATGTT 
ATGGCCATAG CGAACTCGAC AGGATTGGAG GATATCTCCT TCAGTAATGC CACCATGTGC 
GAGAGGACCG CCGTTAAGGG TGAAGACAAC GAGGAATTGG TAAAGCTGCT CAGCGAAATG 
GAGGTGTTAA AACGTGACTT CTTGGCCGAA CAGCAGCGGT GCACCGAGCT GGAGGAGCAG 
TTGGTGACCA TAATCCAAGA CAACCAAGGT CTGCAGACAC GTCTGCTGGA GAACAGTGCC 
AATGAGGGAA CAATGTCGAT GCACGAGGAG TTCAGCCTCC TGGACGACGT GAGACAAGGC 
CAAATGTGTA GTCGCTGCCT GAGGGATATT AACGAAAGCA ACACCAATAT GGATGATCAA 
TCCTCCATTG CCCCAACCGA AGAAATCTAC GAAGATGACG ATCGCAGCAT ACTAAGCGAA 
AGCACCTCGA AATGCGATAA TAGTGGTGCG GATTATAAGG AACGCTTCCG GATTCCCGAG 
GACCTCAATC CCAACTCTAG CGACAAACCA AATCCTTATC GGGACCTAGT CGAGAAATAT 
GAAGCTCTGG TGGAGGTGAA GAGAACCTCG AATGCGGTCA AGAGCAACTT CACCAGCAAT 
CCAGATGGGA AGACCATGAC CGAGTCCAGT CAAGGCAAAA AATCAGAAAC AATTGTTAAC 
AGCTCAAAGG AATCTGACCT TATGTTGGAT TCAACACGTA AGCGCACTCC AACCGAGTTT 
TCGGAATCAG AAACTACATC GTCGGGATTC TCGGATGAAA CTAGCAACAA GTCTACGCAA 
ACTGACGAGC GACCGAGTTA CTTCCTTTGC TCAATAAGTA ATGGAAACGA TTGTAAATTC 
AGTATTTATG ACGACGTCAG CCCCATTGAA TCTCATTTCC GTAATCGCCC GGAGTACAGG 
GAACTCTTCA AGGAGATATT TGGAGTGCTC AAGAAGGCAG CTGATAATAA CGAAGAAGAC 
AAGCTTCCGT CCCTGCATGA CGACGCACAG ATTACAGAAA AATTACCCTT GGTGGCGGCC 
AAAGTACCCC CGGTAACACC CGAGAGGGAG GAATCTCCAG ATGACTTCAT TGACGACACA 
CAGAGCGTCG TATCCTCAGT TATATCCAAC CAGTCCATTG CAATGTCCGA GTGCGTTACC 
AAACTGGAGC GGAAGACAGC CAAAAAACAC ATCTTTGATG TCCGAAACCA ACAAAACCAA 
TCGTCCCTAA CGCAATCCAC TTTGTTAAAC AGCTCCTCGA AGGAAACAAC TGTCGGCGAA 
CCGAATTATA AACCCATAAG GGAGAATGGC CAGATACTTA CTCCACTCAA GCGCGAACCA 
CTCGAATATC TAACCGTAGG TGTCGGAATC AAGAAGAAGA ACCGACGAAA GCACCGAAAC 
CAAAGCTCAT CTGGAGATCG CATCGAATTG TTTAACTCCC GGGAATTTAC TCCCAGGAAC 
AGTCCTCTGG CCATGAACAA CCGTGGTGGT GGACAAGGGA GCTCAAAGAT GTGTACGGAT 
ACATTGAATG CTGAATTCGG ACGCAGTAAC CGCAGACGGA CAACTCCATC CTCAAGCAAC 
TGGAATGGCT CTCCCATGGT GATCTATAAC AAGAATATGA ATACTCCCCA AACTTCTCGG 
GGCCGAGTAA TAGAGCTCAA TGGCGTCGAG TTTTATCATA ACACGGTATC GCAAGACTTA 
CATAAACTTA AAAAGTTAGA CTTATCCTAC GCCGAAGTTT TGCGCCGGGC CGATGCCGGC 
GAACATGGAC CCACAAGATC ACATTCCCAG CGACAGCAAC ACAACGGAGC CAACATTCGC 
AAGAGTCATC ATCATCAGTT TCGTCAAAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724279.1
CDS	135..2507
Translation	MEESNHGSAGCENVSQFMLDDLQLAAELGKTLLERNKELETFIKEYKIKGDEQELEILHLRKHINAMTEVNDSRLKVYEQLEVGIQDLERANQRLNLEKNRDKKQIKTLTTNTEVLEARCEELSQLLSDARQSLSTERRKVDQYQQERYRMQHSTEGSVSSHSIQSLCKEQSVEFSKLDVMAIANSTGLEDISFSNATMCERTAVKGEDNEELVKLLSEMEVLKRDFLAEQQRCTELEEQLVTIIQDNQGLQTRLLENSANEGTMSMHEEFSLLDDVRQGQMCSRCLRDINESNTNMDDQSSIAPTEEIYEDDDRSILSESTSKCDNSGADYKERFRIPEDLNPNSSDKPNPYRDLVEKYEALVEVKRTSNAVKSNFTSNPDGKTMTESSQGKKSETIVNSSKESDLMLDSTRKRTPTEFSESETTSSGFSDETSNKSTQTDERPSYFLCSISNGNDCKFSIYDDVSPIESHFRNRPEYRELFKEIFGVLKKAADNNEEDKLPSLHDDAQITEKLPLVAAKVPPVTPEREESPDDFIDDTQSVVSSVISNQSIAMSECVTKLERKTAKKHIFDVRNQQNQSSLTQSTLLNSSSKETTVGEPNYKPIRENGQILTPLKREPLEYLTVGVGIKKKNRRKHRNQSSSGDRIELFNSREFTPRNSPLAMNNRGGGQGSSKMCTDTLNAEFGRSNRRRTTPSSSNWNGSPMVIYNKNMNTPQTSRGRVIELNGVEFYHNTVSQDLHKLKKLDLSYAEVLRRADAGEHGPTRSHSQRQQHNGANIRKSHHHQFRQK

Target ORF information:

RefSeq Version	NM_165338.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster centrocortin, transcript variant B (Cen), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165338.3

ORF Insert Sequence:

ATGGAGGAAT CCAATCACGG TTCGGCTGGC TGTGAAAACG TATCGCAGTT CATGCTCGAT 
GACCTACAAT TGGCAGCAGA GCTGGGAAAA ACGCTCCTGG AACGCAATAA GGAGCTGGAA 
ACCTTCATCA AGGAGTACAA GATTAAGGGG GATGAGCAGG AACTGGAGAT CTTGCATCTG 
CGCAAGCACA TTAATGCGAT GACCGAGGTG AATGATTCAC GGCTTAAGGT CTACGAGCAG 
CTGGAAGTGG GCATTCAGGA TCTGGAGCGT GCCAATCAGC GGCTAAATCT GGAGAAGAAT 
CGGGACAAGA AGCAGATTAA GACGCTGACC ACCAACACGG AAGTCCTGGA AGCCCGCTGC 
GAGGAACTGA GTCAACTTCT GAGCGATGCT CGGCAATCCC TGAGCACCGA GCGGCGGAAG 
GTGGATCAGT ACCAGCAGGA ACGCTATAGG ATGCAGCATT CGACCGAGGG TTCTGTGTCC 
AGTCACAGCA TTCAATCGCT GTGCAAGGAG CAGAGTGTTG AGTTCTCCAA ACTAGATGTT 
ATGGCCATAG CGAACTCGAC AGGATTGGAG GATATCTCCT TCAGTAATGC CACCATGTGC 
GAGAGGACCG CCGTTAAGGG TGAAGACAAC GAGGAATTGG TAAAGCTGCT CAGCGAAATG 
GAGGTGTTAA AACGTGACTT CTTGGCCGAA CAGCAGCGGT GCACCGAGCT GGAGGAGCAG 
TTGGTGACCA TAATCCAAGA CAACCAAGGT CTGCAGACAC GTCTGCTGGA GAACAGTGCC 
AATGAGGGAA CAATGTCGAT GCACGAGGAG TTCAGCCTCC TGGACGACGT GAGACAAGGC 
CAAATGTGTA GTCGCTGCCT GAGGGATATT AACGAAAGCA ACACCAATAT GGATGATCAA 
TCCTCCATTG CCCCAACCGA AGAAATCTAC GAAGATGACG ATCGCAGCAT ACTAAGCGAA 
AGCACCTCGA AATGCGATAA TAGTGGTGCG GATTATAAGG AACGCTTCCG GATTCCCGAG 
GACCTCAATC CCAACTCTAG CGACAAACCA AATCCTTATC GGGACCTAGT CGAGAAATAT 
GAAGCTCTGG TGGAGGTGAA GAGAACCTCG AATGCGGTCA AGAGCAACTT CACCAGCAAT 
CCAGATGGGA AGACCATGAC CGAGTCCAGT CAAGGCAAAA AATCAGAAAC AATTGTTAAC 
AGCTCAAAGG AATCTGACCT TATGTTGGAT TCAACACGTA AGCGCACTCC AACCGAGTTT 
TCGGAATCAG AAACTACATC GTCGGGATTC TCGGATGAAA CTAGCAACAA GTCTACGCAA 
ACTGACGAGC GACCGAGTTA CTTCCTTTGC TCAATAAGTA ATGGAAACGA TTGTAAATTC 
AGTATTTATG ACGACGTCAG CCCCATTGAA TCTCATTTCC GTAATCGCCC GGAGTACAGG 
GAACTCTTCA AGGAGATATT TGGAGTGCTC AAGAAGGCAG CTGATAATAA CGAAGAAGAC 
AAGCTTCCGT CCCTGCATGA CGACGCACAG ATTACAGAAA AATTACCCTT GGTGGCGGCC 
AAAGTACCCC CGGTAACACC CGAGAGGGAG GAATCTCCAG ATGACTTCAT TGACGACACA 
CAGAGCGTCG TATCCTCAGT TATATCCAAC CAGTCCATTG CAATGTCCGA GTGCGTTACC 
AAACTGGAGC GGAAGACAGC CAAAAAACAC ATCTTTGATG TCCGAAACCA ACAAAACCAA 
TCGTCCCTAA CGCAATCCAC TTTGTTAAAC AGCTCCTCGA AGGAAACAAC TGTCGGCGAA 
CCGAATTATA AACCCATAAG GGAGAATGGC CAGATACTTA CTCCACTCAA GCGCGAACCA 
CTCGAATATC TAACCGTAGG TGTCGGAATC AAGAAGAAGA ACCGACGAAA GCACCGAAAC 
CAAAGCTCAT CTGGAGATCG CATCGAATTG TTTAACTCCC GGGAATTTAC TCCCAGGAAC 
AGTCCTCTGG CCATGAACAA CCGTGGTGGT GGACAAGGGA GCTCAAAGAT GTGTACGGAT 
ACATTGAATG CTGAATTCGG ACGCAGTAAC CGCAGACGGA CAACTCCATC CTCAAGCAAC 
TGGAATGGCT CTCCCATGGT GATCTATAAC AAGAATATGA ATACTCCCCA AACTTCTCGG 
GGCCGAGTAA TAGAGCTCAA TGGCGTCGAG TTTTATCATA ACACGGTATC GCAAGACTTA 
CATAAACTTA AAAAGTTAGA CTTATCCTAC GCCGAAGTTT TGCGCCGGGC CGATGCCGGC 
GAACATGGAC CCACAAGATC ACATTCCCAG CGACAGCAAC ACAACGGAGC CAACATTCGC 
AAGAGTCATC ATCATCAGTT TCGTCAAAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Cen cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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