Irk3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Irk3
Entrez Gene ID	35131
Full Name	Inwardly rectifying potassium channel 3
Synonyms	CG10369,Dmel\CG10369,dKirIII,irk3
General protein information	Preferred Names Inwardly rectifying potassium channel 3 Names CG10369 gene product from transcript CG10369-RA CG10369-PA CG10369-PC CG10369-PD Irk3-PA Irk3-PC Irk3-PD inwardly rectifying potassium channel 3
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 37A1-37A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001144363.2	NP_001137835.2	inwardly rectifying potassium channel 3, isoform D
NM_136059.4	NP_609903.2	inwardly rectifying potassium channel 3, isoform A
NM_001299129.1	NP_001286058.1	inwardly rectifying potassium channel 3, isoform C

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Irk3	NP_609903.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007818	XP_555705.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Irk3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Irk3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14920	NM_136059.4 Latest version!	Drosophila melanogaster inwardly rectifying potassium channel 3, transcript variant A (Irk3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa21679	NM_001144363.2 Latest version!	Drosophila melanogaster inwardly rectifying potassium channel 3, transcript variant D (Irk3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa21680	NM_001299129.1 Latest version!	Drosophila melanogaster inwardly rectifying potassium channel 3, transcript variant C (Irk3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Irk3 ( NM_136059.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609903.2 Drosophila melanogaster inwardly rectifying potassium channel 3, transcript variant A (Irk3), mRNA.

Price & Availability↑

CloneID	OFa14920
Clone ID Related Accession (Same CDS sequence)	NM_136059.4
Accession Version	NM_136059.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	inwardly rectifying potassium channel 3, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_136059.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAATCGG ACGCATCTCC CCTGGGAGCT GCTGCCACGG ATTCCCTAAC CATGCATCGC 
TCAACCTCCA TGCCGGTGAA GCCCAAGCCA CTGGAGCAGA AGCCACTGCT CCGTTCCTCC 
GAAAAGGAGA CGGTCAATGC GTCCGATTAC TATCCGGAGA GTCCGGGATT CGTACGGCGC 
AGAAGGTCAA AAGCAGCCGG CGATCACCTG GAAACACGCA GCGAGCAAAA TTTCCCCTAC 
GCACACGACT GGGCGGGCAG TACGATTGAG CTTACTCCAG ATTTGGGATC CGGACCGCGG 
ACTTCCTCCT CCGATGGACT GAGACGCAGC CTTAATCGCG TGATGGAAAA GAACGGCAAG 
GAGAACGTTG TGTTCCGCCG CATTCCGGAG AAGTCATGGC GCTACATGCG GGATCTGGTC 
ACCACGCTGA TGGAACTGGA GTGGAAGTAT ATGCTGACCC TGTTCCTGGG CAGCTACTTC 
CTCAGCTGGC TGCTCTTCGC CGCCCTCTGC TACGTGGTGG CCTACTCGCA CGGTGACTTC 
ATCTTCGATC CGGTAAGTGG CAAGCGAATG GGCGAGGGCG TGGATCCCTG CATCTACGGG 
GTGCACAGCT GGGTGGCCAT GATCATATAC TCTGTGGAAA CACAGACGAC CCTCGGATTC 
GGCGAAAAAT ACGCCAGCGA GGAGTGTCCG GAGACGATCT TCCTGTTCGT CATGCAGATG 
TTGAGTGCGG CCCTAATCGA GGGCTGTATG GTGAGCGTGA TCTATGCCAA GACCGCACGG 
CCGGCGAGGC AGCTGACCAA GCTGAAGTTC AGCGATAAGG CGGTGATCTG TTATCGCGAC 
GGCAGGCTTT GTTTGCTCTT CCGCGTCTGT GATCCCCGCG AGCAGCAGTC CATCGAGTCC 
AAGATACGAG TCTACATCAT CGTGGACAAA CGCACGCGCG AGGGTGAGAC TATAAAAAGT 
CACGTGGAAC TGAAGCTGGA GGGGAATGGG GAGCAGATTA TTCTCTGGCC CGATGTGGTG 
TGCCATGTCA TCGATGAGAC CAGCCCGTTG TCTCAGTTCA CCACAGCGAA ACTCTTCAAT 
GCTGCCCAAT TTGAGTTGTA TGTGTCCATT GTGGGCACTT CGCCTGCCAC AGCGCAGATG 
ACGGAGGCCA AGACGTCCTA TCTGCCAAGG GAGATTTTCT GGGGCCAGCG GTTCGTCAAT 
ATCATCCATT ACGACGCCCA AAACGAGCGC TACATTGTCG ACTACGAGAA CTTCAACAGG 
ACCATATCGG TGGACATGCC CATGACGAAT CCGAAAAATG ATCACTTAAA GCTAGAGTAC 
AGAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609903.2
CDS	214..1542
Translation	MQSDASPLGAAATDSLTMHRSTSMPVKPKPLEQKPLLRSSEKETVNASDYYPESPGFVRRRRSKAAGDHLETRSEQNFPYAHDWAGSTIELTPDLGSGPRTSSSDGLRRSLNRVMEKNGKENVVFRRIPEKSWRYMRDLVTTLMELEWKYMLTLFLGSYFLSWLLFAALCYVVAYSHGDFIFDPVSGKRMGEGVDPCIYGVHSWVAMIIYSVETQTTLGFGEKYASEECPETIFLFVMQMLSAALIEGCMVSVIYAKTARPARQLTKLKFSDKAVICYRDGRLCLLFRVCDPREQQSIESKIRVYIIVDKRTREGETIKSHVELKLEGNGEQIILWPDVVCHVIDETSPLSQFTTAKLFNAAQFELYVSIVGTSPATAQMTEAKTSYLPREIFWGQRFVNIIHYDAQNERYIVDYENFNRTISVDMPMTNPKNDHLKLEYRK

Target ORF information:

RefSeq Version	NM_136059.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster inwardly rectifying potassium channel 3, transcript variant A (Irk3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136059.4

ORF Insert Sequence:

ATGCAATCGG ACGCATCTCC CCTGGGAGCT GCTGCCACGG ATTCCCTAAC CATGCATCGC 
TCAACCTCCA TGCCGGTGAA GCCCAAGCCA CTGGAGCAGA AGCCACTGCT CCGTTCCTCC 
GAAAAGGAGA CGGTCAATGC GTCCGATTAC TATCCGGAGA GTCCGGGATT CGTACGGCGC 
AGAAGGTCAA AAGCAGCCGG CGATCACCTG GAAACACGCA GCGAGCAAAA TTTCCCCTAC 
GCACACGACT GGGCGGGCAG TACGATTGAG CTTACTCCAG ATTTGGGATC CGGACCGCGG 
ACTTCCTCCT CCGATGGACT GAGACGCAGC CTTAATCGCG TGATGGAAAA GAACGGCAAG 
GAGAACGTTG TGTTCCGCCG CATTCCGGAG AAGTCATGGC GCTACATGCG GGATCTGGTC 
ACCACGCTGA TGGAACTGGA GTGGAAGTAT ATGCTGACCC TGTTCCTGGG CAGCTACTTC 
CTCAGCTGGC TGCTCTTCGC CGCCCTCTGC TACGTGGTGG CCTACTCGCA CGGTGACTTC 
ATCTTCGATC CGGTAAGTGG CAAGCGAATG GGCGAGGGCG TGGATCCCTG CATCTACGGG 
GTGCACAGCT GGGTGGCCAT GATCATATAC TCTGTGGAAA CACAGACGAC CCTCGGATTC 
GGCGAAAAAT ACGCCAGCGA GGAGTGTCCG GAGACGATCT TCCTGTTCGT CATGCAGATG 
TTGAGTGCGG CCCTAATCGA GGGCTGTATG GTGAGCGTGA TCTATGCCAA GACCGCACGG 
CCGGCGAGGC AGCTGACCAA GCTGAAGTTC AGCGATAAGG CGGTGATCTG TTATCGCGAC 
GGCAGGCTTT GTTTGCTCTT CCGCGTCTGT GATCCCCGCG AGCAGCAGTC CATCGAGTCC 
AAGATACGAG TCTACATCAT CGTGGACAAA CGCACGCGCG AGGGTGAGAC TATAAAAAGT 
CACGTGGAAC TGAAGCTGGA GGGGAATGGG GAGCAGATTA TTCTCTGGCC CGATGTGGTG 
TGCCATGTCA TCGATGAGAC CAGCCCGTTG TCTCAGTTCA CCACAGCGAA ACTCTTCAAT 
GCTGCCCAAT TTGAGTTGTA TGTGTCCATT GTGGGCACTT CGCCTGCCAC AGCGCAGATG 
ACGGAGGCCA AGACGTCCTA TCTGCCAAGG GAGATTTTCT GGGGCCAGCG GTTCGTCAAT 
ATCATCCATT ACGACGCCCA AAACGAGCGC TACATTGTCG ACTACGAGAA CTTCAACAGG 
ACCATATCGG TGGACATGCC CATGACGAAT CCGAAAAATG ATCACTTAAA GCTAGAGTAC 
AGAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Irk3 ( NM_001144363.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137835.2 Drosophila melanogaster inwardly rectifying potassium channel 3, transcript variant D (Irk3), mRNA.

Price & Availability↑

CloneID	OFa21679
Clone ID Related Accession (Same CDS sequence)	NM_001144363.2
Accession Version	NM_001144363.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	inwardly rectifying potassium channel 3, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001144363.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAATCGG ACGCATCTCC CCTGGGAGCT GCTGCCACGG ATTCCCTAAC CATGCATCGC 
TCAACCTCCA TGCCGGTGAA GCCCAAGCCA CTGGAGCAGA AGCCACTGCT CCGTTCCTCC 
GAAAAGGAGA CGGTCAATGC GTCCGATTAC TATCCGGAGA GTCCGGGATT CGTACGGCGC 
AGAAGGTCAA AAGCAGCCGG CGATCACCTG GAAACACGCA GCGAGCAAAA TCCCACGTTT 
AGTTTCCCCT ACGCACACGA CTGGGCGGGC AGTACGATTG AGCTTACTCC AGATTTGGGA 
TCCGGACCGC GGACTTCCTC CTCCGATGGA CTGAGACGCA GCCTTAATCG CGTGATGGAA 
AAGAACGGCA AGGAGAACGT TGTGTTCCGC CGCATTCCGG AGAAGTCATG GCGCTACATG 
CGGGATCTGG TCACCACGCT GATGGAACTG GAGTGGAAGT ATATGCTGAC CCTGTTCCTG 
GGCAGCTACT TCCTCAGCTG GCTGCTCTTC GCCGCCCTCT GCTACGTGGT GGCCTACTCG 
CACGGTGACT TCATCTTCGA TCCGGTAAGT GGCAAGCGAA TGGGCGAGGG CGTGGATCCC 
TGCATCTACG GGGTGCACAG CTGGGTGGCC ATGATCATAT ACTCTGTGGA AACACAGACG 
ACCCTCGGAT TCGGCGAAAA ATACGCCAGC GAGGAGTGTC CGGAGACGAT CTTCCTGTTC 
GTCATGCAGA TGTTGAGTGC GGCCCTAATC GAGGGCTGTA TGGTGAGCGT GATCTATGCC 
AAGACCGCAC GGCCGGCGAG GCAGCTGACC AAGCTGAAGT TCAGCGATAA GGCGGTGATC 
TGTTATCGCG ACGGCAGGCT TTGTTTGCTC TTCCGCGTCT GTGATCCCCG CGAGCAGCAG 
TCCATCGAGT CCAAGATACG AGTCTACATC ATCGTGGACA AACGCACGCG CGAGGGTGAG 
ACTATAAAAA GTCACGTGGA ACTGAAGCTG GAGGGGAATG GGGAGCAGAT TATTCTCTGG 
CCCGATGTGG TGTGCCATGT CATCGATGAG ACCAGCCCGT TGTCTCAGTT CACCACAGCG 
AAACTCTTCA ATGCTGCCCA ATTTGAGTTG TATGTGTCCA TTGTGGGCAC TTCGCCTGCC 
ACAGCGCAGA TGACGGAGGC CAAGACGTCC TATCTGCCAA GGGAGATTTT CTGGGGCCAG 
CGGTTCGTCA ATATCATCCA TTACGACGCC CAAAACGAGC GCTACATTGT CGACTACGAG 
AACTTCAACA GGACCATATC GGTGGACATG CCCATGACGA ATCCGAAAAA TGATCACTTA 
AAGCTAGAGT ACAGAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137835.2
CDS	214..1554
Translation	MQSDASPLGAAATDSLTMHRSTSMPVKPKPLEQKPLLRSSEKETVNASDYYPESPGFVRRRRSKAAGDHLETRSEQNPTFSFPYAHDWAGSTIELTPDLGSGPRTSSSDGLRRSLNRVMEKNGKENVVFRRIPEKSWRYMRDLVTTLMELEWKYMLTLFLGSYFLSWLLFAALCYVVAYSHGDFIFDPVSGKRMGEGVDPCIYGVHSWVAMIIYSVETQTTLGFGEKYASEECPETIFLFVMQMLSAALIEGCMVSVIYAKTARPARQLTKLKFSDKAVICYRDGRLCLLFRVCDPREQQSIESKIRVYIIVDKRTREGETIKSHVELKLEGNGEQIILWPDVVCHVIDETSPLSQFTTAKLFNAAQFELYVSIVGTSPATAQMTEAKTSYLPREIFWGQRFVNIIHYDAQNERYIVDYENFNRTISVDMPMTNPKNDHLKLEYRK

Target ORF information:

RefSeq Version	NM_001144363.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster inwardly rectifying potassium channel 3, transcript variant D (Irk3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144363.2

ORF Insert Sequence:

ATGCAATCGG ACGCATCTCC CCTGGGAGCT GCTGCCACGG ATTCCCTAAC CATGCATCGC 
TCAACCTCCA TGCCGGTGAA GCCCAAGCCA CTGGAGCAGA AGCCACTGCT CCGTTCCTCC 
GAAAAGGAGA CGGTCAATGC GTCCGATTAC TATCCGGAGA GTCCGGGATT CGTACGGCGC 
AGAAGGTCAA AAGCAGCCGG CGATCACCTG GAAACACGCA GCGAGCAAAA TCCCACGTTT 
AGTTTCCCCT ACGCACACGA CTGGGCGGGC AGTACGATTG AGCTTACTCC AGATTTGGGA 
TCCGGACCGC GGACTTCCTC CTCCGATGGA CTGAGACGCA GCCTTAATCG CGTGATGGAA 
AAGAACGGCA AGGAGAACGT TGTGTTCCGC CGCATTCCGG AGAAGTCATG GCGCTACATG 
CGGGATCTGG TCACCACGCT GATGGAACTG GAGTGGAAGT ATATGCTGAC CCTGTTCCTG 
GGCAGCTACT TCCTCAGCTG GCTGCTCTTC GCCGCCCTCT GCTACGTGGT GGCCTACTCG 
CACGGTGACT TCATCTTCGA TCCGGTAAGT GGCAAGCGAA TGGGCGAGGG CGTGGATCCC 
TGCATCTACG GGGTGCACAG CTGGGTGGCC ATGATCATAT ACTCTGTGGA AACACAGACG 
ACCCTCGGAT TCGGCGAAAA ATACGCCAGC GAGGAGTGTC CGGAGACGAT CTTCCTGTTC 
GTCATGCAGA TGTTGAGTGC GGCCCTAATC GAGGGCTGTA TGGTGAGCGT GATCTATGCC 
AAGACCGCAC GGCCGGCGAG GCAGCTGACC AAGCTGAAGT TCAGCGATAA GGCGGTGATC 
TGTTATCGCG ACGGCAGGCT TTGTTTGCTC TTCCGCGTCT GTGATCCCCG CGAGCAGCAG 
TCCATCGAGT CCAAGATACG AGTCTACATC ATCGTGGACA AACGCACGCG CGAGGGTGAG 
ACTATAAAAA GTCACGTGGA ACTGAAGCTG GAGGGGAATG GGGAGCAGAT TATTCTCTGG 
CCCGATGTGG TGTGCCATGT CATCGATGAG ACCAGCCCGT TGTCTCAGTT CACCACAGCG 
AAACTCTTCA ATGCTGCCCA ATTTGAGTTG TATGTGTCCA TTGTGGGCAC TTCGCCTGCC 
ACAGCGCAGA TGACGGAGGC CAAGACGTCC TATCTGCCAA GGGAGATTTT CTGGGGCCAG 
CGGTTCGTCA ATATCATCCA TTACGACGCC CAAAACGAGC GCTACATTGT CGACTACGAG 
AACTTCAACA GGACCATATC GGTGGACATG CCCATGACGA ATCCGAAAAA TGATCACTTA 
AAGCTAGAGT ACAGAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Irk3 ( NM_001299129.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286058.1 Drosophila melanogaster inwardly rectifying potassium channel 3, transcript variant C (Irk3), mRNA.

Price & Availability↑

CloneID	OFa21680
Clone ID Related Accession (Same CDS sequence)	NM_001299129.1
Accession Version	NM_001299129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	inwardly rectifying potassium channel 3, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAATCGG ACGCATCTCC CCTGGGAGCT GCTGCCACGG ATTCCCTAAC CATGCATCGC 
TCAACCTCCA TGCCGGTGAA GCCCAAGCCA CTGGAGCAGA AGCCACTGCT CCGTTCCTCC 
GAAAAGGAGA CGGTCAATGC GTCCGATTAC TATCCGGAGA GTCCGGGATT CGTACGGCGC 
AGAAGGTCAA AAGCAGCCGG CGATCACCTG GAAACACGCA GCGAGCAAAA TTTCCCCTAC 
GCACACGACT GGGCGGGCAG TACGATTGAG CTTACTCCAG ATTTGGGATC CGGACCGCGG 
ACTTCCTCCT CCGATGGACT GAGACGCAGC CTTAATCGCG TGATGGAAAA GAACGGCAAG 
GAGAACGTTG TGTTCCGCCG CATTCCGGAG AAGTCATGGC GCTACATGCG GGATCTGGTC 
ACCACGCTGG TAAGATCCTG GTTCGGACAC AGGAAAACGT CTACACCCAG GGCCGATCAA 
TCGCGATCGG ATGTGTATAG CATAACGCCC AGTATTCAGC GCAGACTCGA TGAACTGGCC 
AATGAGCCAC CGCACCTGAG TCCCCATTTT TCGAAGTCTA ATCCTAACCA AAACGAGCGC 
GTCACGTTCA GCATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286058.1
CDS	214..831
Translation	MQSDASPLGAAATDSLTMHRSTSMPVKPKPLEQKPLLRSSEKETVNASDYYPESPGFVRRRRSKAAGDHLETRSEQNFPYAHDWAGSTIELTPDLGSGPRTSSSDGLRRSLNRVMEKNGKENVVFRRIPEKSWRYMRDLVTTLVRSWFGHRKTSTPRADQSRSDVYSITPSIQRRLDELANEPPHLSPHFSKSNPNQNERVTFSI

Target ORF information:

RefSeq Version	NM_001299129.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster inwardly rectifying potassium channel 3, transcript variant C (Irk3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299129.1

ORF Insert Sequence:

ATGCAATCGG ACGCATCTCC CCTGGGAGCT GCTGCCACGG ATTCCCTAAC CATGCATCGC 
TCAACCTCCA TGCCGGTGAA GCCCAAGCCA CTGGAGCAGA AGCCACTGCT CCGTTCCTCC 
GAAAAGGAGA CGGTCAATGC GTCCGATTAC TATCCGGAGA GTCCGGGATT CGTACGGCGC 
AGAAGGTCAA AAGCAGCCGG CGATCACCTG GAAACACGCA GCGAGCAAAA TTTCCCCTAC 
GCACACGACT GGGCGGGCAG TACGATTGAG CTTACTCCAG ATTTGGGATC CGGACCGCGG 
ACTTCCTCCT CCGATGGACT GAGACGCAGC CTTAATCGCG TGATGGAAAA GAACGGCAAG 
GAGAACGTTG TGTTCCGCCG CATTCCGGAG AAGTCATGGC GCTACATGCG GGATCTGGTC 
ACCACGCTGG TAAGATCCTG GTTCGGACAC AGGAAAACGT CTACACCCAG GGCCGATCAA 
TCGCGATCGG ATGTGTATAG CATAACGCCC AGTATTCAGC GCAGACTCGA TGAACTGGCC 
AATGAGCCAC CGCACCTGAG TCCCCATTTT TCGAAGTCTA ATCCTAACCA AAACGAGCGC 
GTCACGTTCA GCATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Irk3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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