ck cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ck
Entrez Gene ID	34882
Full Name	crinkled
Synonyms	53D10S,BG:DS00929.11,CG7595,D,D2,DM7a,DMyoVIIa,Dm 35B,DmVIIA,DmVIIa,Dmel\CG7595,Dro35B,ESTS:53D10S,Mhc35BC,Myo7A,Myo7B,MyoVIIA,anon-35Bb,br27,ck/MyoVIIA,l(1)35Ca,l(2)07130,l(2)35Ca,l(2)br27,l35Ca,myoVIIA,stc,stch
General protein information	Preferred Names crinkled Names CG7595 gene product from transcript CG7595-RB CG7595-PA CG7595-PB CG7595-PC CG7595-PD ck-PA ck-PB ck-PC ck-PD lethal 27 in the black-reduced region lethal group D2 myosin 7a myosin 7a heavy chain myosin VII myosin VIIa myosin heavy chain at 35BC myosin-VII mysoin VIIa stubby chaetae transcription unit D
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 35B8-35B9\|2-51.0 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001299020.1	NP_001285949.1	crinkled, isoform C
NM_165099.3	NP_723895.1	crinkled, isoform A
NM_001299021.1	NP_001285950.1	crinkled, isoform D
NM_078847.4	NP_523571.1	crinkled, isoform B

Pathways from BioSystems

Homologies

	MYO7A	XP_001087868.2
Gallus gallus (chicken)	MYO7A	XP_004938998.1
Danio rerio (zebrafish)	myo7aa	NP_694515.1
Rattus norvegicus (Norway rat)	Myo7a	NP_703203.1
Xenopus tropicalis (tropical clawed frog)	myo7a	XP_004912319.1
Homo sapiens (human)	MYO7A	NP_000251.3
Bos taurus (cattle)	MYO7A	XP_005216396.1
Pan troglodytes (chimpanzee)	MYO7A	XP_003313297.2
Canis lupus familiaris (dog)	MYO7A	XP_005633514.1
Mus musculus (house mouse)	Myo7a	NP_001243010.1
Drosophila melanogaster (fruit fly)	ck	NP_523571.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CK selectively interacts with the initiator caspase DRONC and regulates some of its non-apoptotic functions. Results expose a conserved role for unconventional myosins in transducing caspase-dependent regulation of kinases.
Title: The unconventional myosin CRINKLED and its mammalian orthologue MYO7A regulate caspases in their signalling roles.

Data suggest that Crinkled acts in concert with Wingless targets to orchestrate the proper shaping of denticles in the Drosophila embryonic epidermis.
Title: crinkled reveals a new role for Wingless signaling in Drosophila denticle formation.

domain markedly inhibits the actin-activated ATPase activity of tailless DM7A at low Ca(2+) but not high Ca(2+)
Title: The tail binds to the head-neck domain, inhibiting ATPase activity of myosin VIIA.

kinetic behavior would allow myosin VIIa to exert and hold tension on actin filaments and, if dimerized, to function as a processive cargo transporter.
Title: Dimerized Drosophila myosin VIIa: a processive motor.

myosin VIIA is classified to be a high duty ratio motor
Title: Drosophila myosin VIIA is a high duty ratio motor with a unique kinetic mechanism.

The following ck gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ck cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15890	NM_078847.4 Latest version!	Drosophila melanogaster crinkled (ck), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa15890	NM_165099.3 Latest version!	Drosophila melanogaster crinkled (ck), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa15890	NM_001299021.1 Latest version!	Drosophila melanogaster crinkled (ck), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa15890	NM_001299020.1 Latest version!	Drosophila melanogaster crinkled (ck), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ck ( NM_001299020.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285949.1 Drosophila melanogaster crinkled (ck), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa15890
Clone ID Related Accession (Same CDS sequence)	NM_001299020.1 , NM_165099.3 , NM_001299021.1 , NM_078847.4
Accession Version	NM_001299020.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	crinkled, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGATCG TAACACGTGG TGACTACATC TGGATAGAGC CCGCATCTGG GCGAGAATTC 
GATGTGGCCA TTGGAGCACG TGTCGTCTCC GCCGAGGGTC GTCGCATCCA GGTGCGCGAT 
GACGATGGCG ACGAGGTGTG GCTGGCACCC GAGCGCCGCA TCAAGGCCAT GCACGCGTCC 
TCCGTTCAGG GTGTGGAGGA CATGATATCC CTGGGTGATC TGCACGAGGC TGGCATCTTG 
CGGAATCTGC TCATCCGCTA CAAGGAGAAT CTGATATACA CTTACACGGG CTCCATACTT 
GTTGCCGTTA ATCCCTACCA GATTCTGCCC ATCTACACAG GTGATCAGAT CAAGCTCTAT 
AAGGAGCGGA AAATCGGTGA GCTACCGCCG CATATCTTTG CAATTGGCGA CAATGCGTAT 
GCGCATATGA AACGATATCG CCAGGATCAG TGTATCGTTA TTTCCGGAGA GTCTGGAGCT 
GGAAAGACGG AGAGCACAAA GTTGATCCTG CAGTATCTGG CTGCGATTAG TGGCAAACAC 
TCATGGATCG AACAGCAAAT CCTTGAAGCG AATCCCATTT TGGAAGCTTT CGGAAATGCC 
AAAACCATTC GAAATGATAA TTCATCGCGT TTTGGCAAAT ACATAGATAT ACACTTTAGC 
GCCAATGGCG TGATCGAGGG AGCCAAAATA GAACAGTACC TGCTGGAGAA GTCGCGAATT 
GTTTCCCAAA ATCATAGCGA ACGAAATTAT CATGTCTTTT ACTGCATTTT GGCCGGACTG 
TCGGCAGATG AGAAGAGTCG TCTCGATCTT GGCATGGCTG CTGATTACAA ATATCTGACT 
GGTGGTAATA GCATAACCTG CGAGGGACGC GATGACGCCG CCGAATTCTC TGACATTCGA 
TCTGCCATGA AGGTTTTGCT CTTCTCCGAT CAGGAAATCT GGGAGATAAT TAAACTCCTT 
GCTGCCCTTC TCCACTGCGG CAACATCAAA TATAAGGCGA CTGTGGTGGA TAATCTGGAT 
GCTACCGAAA TACCGGAACA CATAAATGTG GAGCGTGTGG CCGGGTTACT TGGACTTCCC 
ATTCAGCCGC TAATTGATGC TCTAACACGT CGAACACTTT TTGCTCATGG AGAAACCGTG 
GTGTCAACTC TGTCGCGCGA TCAATCCGTG GATGTGCGCG ATGCCTTTGT GAAAGGCATA 
TACGGACGTA TGTTTGTGCA CATCGTTCGA AAGATCAATA CGGCAATTTT CAAGCCGCGC 
GGCACATCCC GAAATGCCAT CGGAGTTCTC GACATCTTTG GTTTTGAGAA CTTCGATCAG 
AATAGCTTTG AGCAGTTTTG CATCAACTAT GCCAATGAGA ATCTGCAGCA GTTCTTCGTG 
CAGCATATAT TTAAACTTGA GCAGGAAGAG TATAACCACG AGGCCATCAA CTGGCAACAC 
ATTGAGTTCG TAGACAATCA GGATGCGCTC GACTTGATAG CTATTAAGCA GCTCAATATT 
ATGGCTTTAA TCGATGAGGA GGCCCGGTTT CCCAAGGGCA CGGATCAGAC GATGCTGGCC 
AAATTGCACA AAACCCATGG ATCGCACAAG AACTATTTGA AGCCCAAGTC GGATATCAAC 
ACCAGCTTTG GACTGAATCA CTTTGCCGGT GTGGTGTTCT ACGACACACG AGGATTTCTA 
GATAAAAACC GGGACACCTT CAGTCCCGAT CTATTGCATT TGGTTAGCCA AAGTACGAAC 
AAGTTCCTCC GACAAATCTT TGCTCAGGAC ATAGAGATGG GTGCAGAGAC GCGAAAGCGA 
ACACCCACAC TCTCCACACA GTTTCGAAAA TCTCTCGATG CGCTTATGAA GACGCTCAGC 
AGTTGCCAGC CATTCTTTAT TCGTTGTATC AAACCCAACG AGCTGAAGAA GCCAATGATG 
TTTGACCGCG GCTTGTGTTG CCGTCAGCTG CGATATTCCG GCATGATGGA AACGATCCGA 
ATCCGTCGCG CTGGATATCC CATTCGACAT GGATTCAGAG AGTTCGTCGA GCGTTATCGC 
TTCCTGATAC CGGGAGTTCC ACCAGCTCAT CGCACGGATT GCCAGGCGGC GACTTCGAGG 
ATTTGTGCCG TGGTTTTGGG CAAGTCCGAC TATCAGCTGG GCCACACCAA GGTCTTTCTC 
AAGGACGCCC ACGATCTGTT TCTGGAGCAG GAGAGGGATC GCGTGCTGAC GCGAAAAATC 
CTCATTCTGC AGCGCAGCAT TCGTGGTTGG GTGTACCGAC GAAGGTTTCT ACGCCTGAGA 
GCTGCTGCTA TCACTGTGCA GCGATTCTGG AAGGGCTATG CTCAACGTAA ACGATACAGG 
AACATGCGAG TGGGCTACAT GCGTTTGCAA GCGTTGATCC GTTCGAGAGT TCTGTCACAT 
CGTTTCCGCC ATCTGAGGGG CCACATTGTC GGGTTGCAAG CCCATGCCCG AGGATATTTG 
GTGAGGAGAG AGTATGGTCA CAAGATGTGG GCCGTCATTA AGATCCAGTC TCATGTGCGG 
CGTATGATTG CCATGCGGCG CTACCGAAAA CTTCGATTGG AGCACAAACA GTTTGCCGAA 
GTTCTTCAGC TGCGCAAACT GGAGGAGCAG GAACTGCTGC ATCGTGGCAA TAAGCATGCC 
CGAGAAATTG CCGAGCAGCA TTACCGAGAT CGATTGCACG AGCTGGAGCG TCGGGAAATT 
CAGGAGCAAT TGGAGAATCG TCGTCGAGTG GAGGTCAATA TGAATATTAT CAATGATGCA 
GCTCGCAAAC AGGAGGAGCC GGTGGACGAT GGCAAACTGG TGGAGGCCAT GTTTGACTTC 
CTTCCCGATT CCAGTAGCGA TGCCCCTACT CCGCATGGTG GACGTGAAAC GTCCGTGTTC 
AACGATCTGC CTCACGCGCA AAACGTCAAC CAGGACGATA TCATTGCACC CATACACATA 
TCCGAGGATG AGGAGGATCT ATCGGAATTC AAGTTCCAGA AATTCGCTGC CACCTACTTC 
CAAGGAAATG TCAATCATCA GTATGCTAAG AAAGCTTTGA AGCATCCCCT ACTTCCGCTC 
CATACGCAAG GCGATCAACT TGCTGCACAG GCATTGTGGA TAACCATACT GAGGTTTACA 
GGTGATATGC CGGAGCCGAA GTATCACACA ATGGATCGTA TGGACACCAC TTCGGTCATG 
TCCAAGGTCA CTGCCACCTT GGGGCGCAAT TTCATCAGGA GTAAGGAGTT CCAAGAGGCT 
CAGCTTATGG GCCTGGATCC AGATGCATTC CTTAAGCAAA AGCCACGATC GATCCGCCAC 
AAACTCGTAT CCCTGACGTT GAAGCGAAAG AACAAATTGG GCGAGGATGT GCGTCGCCGA 
CTGCAAGATG ATGAATACAC GGCGGATAGC TACCAATCGT GGCTACAATC CCGTCCAACC 
TCCAACCTTG AAAAGCTGCA TTTTATCATT GGCCATGGTA TCCTGCGTGC CGAGCTGCGT 
GACGAAATAT ACTGCCAGAT ATGCAAGCAG CTGACGAACA ATCCATTGAA ATCATCACAT 
GCCAGAGGTT GGATTCTGTT ATCCCTGTGC GTCGGATGTT TTGCTCCGTC GGAAAAGTTT 
GTTAACTACT TGAGGGCCTT TATCCGTGAG GGTCCACCTG GATATGCACC GTACTGCGAG 
GAGCGACTGA AGCGTACTTT CAACAATGGA ACTCGCAATC AACCGCCTTC GTGGCTGGAG 
TTGCAGGCCA CCAAATCCAA AAAGCCCATC ATGCTGCCCA TTACCTTTAT GGATGGAAAT 
ACAAAGACTC TGTTGGCTGA TTCCGCCACC ACGGCCCGAG AACTATGCAA TCAACTGTCC 
GACAAGATAA GTCTCAAAGA TCAGTTTGGT TTTTCCCTGT ACATAGCTCT GTTCGACAAA 
GTTAGTTCTT TGGGAAGTGG CGGGGACCAC GTGATGGACG CCATCTCACA ATGTGAGCAG 
TATGCAAAGG AGCAGGGCGC CCAGGAGCGG AATGCCCCGT GGCGTCTGTT TTTCCGCAAG 
GAAATCTTTG CACCTTGGCA CGAACCCACA CACGATCAGG TGGCCACCAA TCTTATTTAC 
CAGCAGGTGG TGAGAGGCGT TAAGTTTGGC GAATATCGCT GCGACAAGGA GGAAGACTTG 
GCCATGATAG CTGCGCAGCA ATATTTCATC GAATACTCAA CCGATATGTC AATGGAGCGG 
CTGTTTACCC TTCTGCCCAA CTTTATACCC GACTTTTGTC TCAGCGGAGT GGACAAGGCC 
ATTGAACGTT GGGCTGCTTT GGTTCTGCAG GCCTATAAAA AATCGTACTA TGTCAAGGAC 
AAGATCGCAC CCTTGAAAAT TAAGGAGGAT ATAGTTAGCT ACGCCAAGTA CAAGTGGCCT 
CTGCTCTTTT CTCGTTTCTA CGAAGCGTAT CGAAACTCCG GTCCGAATCT GCCCAAAAAC 
GATGTCATCA TTGCGGTGAA CTGGACCGGC GTGTATGTGG TTGATGATCA GGAGCAGGTG 
CTGTTGGAAC TGAGCTTCCC CGAGATTACT GCCGTGTCCA GTCAGAAGAC CAACAAGGTC 
TTCACCCAGA CTTTCAGCCT TTCCACTGTT CGAGGCGAGG AGTTCACCTT CCAGAGCCCC 
AATGCGGAGG ATATCCGTGA TCTGGTTGTC TACTTCTTAG ATGGCCTGAA GAAACGATCG 
AAATATGTCA TAGCCTTGCA GGACTATCGT GCGCCTTCGG ATGGAACAAG TTTTCTGTCT 
TTCTTTAAGG GAGATCTGAT CATACTAGAG GACGAATCCT GTGGAGAATC AGTACTGAAC 
AACGGCTGGT GCATAGGACG CTGCGATCGT TCCCAGGAGC GAGGAGACTT CCCCGCCGAA 
ACCGTGTACG TGCTGCCCAC CCTTAGCAAA CCGCCGCAGG ATATTTTGGC CCTGTTCAAC 
ATCGAGGAGG CGCATCATGG ACGACGTCTA AGCATGGCTT CCAATGGTGG AGCTGTGGAA 
CCAAGGGATC GACCTCATAC GCTGATGGAG TATGCCCTGG ATCACTTCCG TTTGCCACCG 
AAACGAACAA TGTCGAAAAC ACTCACTCTG AGTTCAAAAC GGAGTGAGGA ATTGTGGCGC 
TACTCCCGGG ATCCCATTAA GGCACCGCTG CTGCGCAAGT TGCAAAGCAA GGAAGAGTTT 
GCCGAGGAGG CCTGCTTCGC GTTTGCCGCC ATACTGAAAT ATATGGGCGA TCTGCCCTCC 
AAGCGTCCGC GGATGGGCAA CGAAATAACC GATCACATAT TTGATGGACC TCTGAAGCAC 
GAAATTCTGC GCGACGAGAT CTACTGCCAA CTGATGAAGC AGCTAACGGA CAATCGCAAC 
CGAATGTCCG AGGAGAGGGG CTGGGAGCTG ATGTGGTTGG CCACGGGACT GTTTGCCTGT 
TCACAGGGTT TGCTCAAGGA ATTGCTATTA TTCCTGCGCA CCAGGCGTCA TCCCATTTCA 
CAGGACTCAA TGCATCGACT ACAGAAAACT ATACGCCATG GACAACGCAA ATATCCGCCG 
CATCAGGTGG AGGTGGAAGC CATACAGCAT AAAACCACAC AGATCTTCCA CAAGGTCTAC 
TTCCCGGACG ACACAGACGA GGCCTTCGAA GTGGACAGCT CCACTCGAGC GAAGGATTTC 
TGCAACAACA TCTCGCAGCG CCTTTCGCTG CGCACCAGCG AGGGCTTCTC CCTGTTTGTA 
AAGATCGCGG ATAAGGTCAT CTCTGTTCCC GAAGGTGATT TCTTCTTCGA TTTCGTACGC 
CATTTGACCG ACTGGATTAA AAAAGCGCGT CCCATACGAG ATGGAGCGAA TCCGCAGTTC 
ACTTACCAGG TCTTCTTCAT GAAGAAACTG TGGACGAACA CAGTGCCAGG CAAGGATCGG 
AATGCCGATC TTATATTCCA CTATCATCAG GAGTTGCCCA AGCTGTTGCG TGGCTATCAC 
AAGTGTTCCA GGGAGGAGGC GGCTAAGCTG GCCGCTCTAG TCTTCCGTGT GCGCTTTGGT 
GAAAATAAAC AAGAACTGCA GGCCATACCA CAAATGCTGA GGGAACTTAT TCCATCGGAT 
ATTATGAAAA TACAGAGCAC TAGCGAATGG AAACGATCGA TTGTTGCATC TTATAACCAG 
GATGGGGGCA TGACTTCCGA AGACGCGAAG GTGGCCTTCT TGAAGATTGT ATATAGATGG 
CCAACTTTTG GCTCCGCTTT CTTTGAGGTG AAGCAAACCA CCGAACCGAA TTATCCCGAA 
ATGCTATTGA TAGCCATTAA CAAACACGGC GTGAGTCTCA TACATCCAGT GACCAAGGAC 
ATTTTGGTTA CGCATCCATT TACACGCATC TCGAATTGGT CATCGGGCAA TACATATTTC 
CACATGACCA TCGGAAATCT GGTTAGGGGC TCGAAATTGT TATGTGAAAC GTCGCTGGGC 
TACAAGATGG ACGATCTGCT GACTTCATAT ATCTCGTTGA TGTTGACCAA TATGAACAAA 
AATCGAACCA TTCGAGCCAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285949.1
CDS	287..6790
Translation	MVIVTRGDYIWIEPASGREFDVAIGARVVSAEGRRIQVRDDDGDEVWLAPERRIKAMHASSVQGVEDMISLGDLHEAGILRNLLIRYKENLIYTYTGSILVAVNPYQILPIYTGDQIKLYKERKIGELPPHIFAIGDNAYAHMKRYRQDQCIVISGESGAGKTESTKLILQYLAAISGKHSWIEQQILEANPILEAFGNAKTIRNDNSSRFGKYIDIHFSANGVIEGAKIEQYLLEKSRIVSQNHSERNYHVFYCILAGLSADEKSRLDLGMAADYKYLTGGNSITCEGRDDAAEFSDIRSAMKVLLFSDQEIWEIIKLLAALLHCGNIKYKATVVDNLDATEIPEHINVERVAGLLGLPIQPLIDALTRRTLFAHGETVVSTLSRDQSVDVRDAFVKGIYGRMFVHIVRKINTAIFKPRGTSRNAIGVLDIFGFENFDQNSFEQFCINYANENLQQFFVQHIFKLEQEEYNHEAINWQHIEFVDNQDALDLIAIKQLNIMALIDEEARFPKGTDQTMLAKLHKTHGSHKNYLKPKSDINTSFGLNHFAGVVFYDTRGFLDKNRDTFSPDLLHLVSQSTNKFLRQIFAQDIEMGAETRKRTPTLSTQFRKSLDALMKTLSSCQPFFIRCIKPNELKKPMMFDRGLCCRQLRYSGMMETIRIRRAGYPIRHGFREFVERYRFLIPGVPPAHRTDCQAATSRICAVVLGKSDYQLGHTKVFLKDAHDLFLEQERDRVLTRKILILQRSIRGWVYRRRFLRLRAAAITVQRFWKGYAQRKRYRNMRVGYMRLQALIRSRVLSHRFRHLRGHIVGLQAHARGYLVRREYGHKMWAVIKIQSHVRRMIAMRRYRKLRLEHKQFAEVLQLRKLEEQELLHRGNKHAREIAEQHYRDRLHELERREIQEQLENRRRVEVNMNIINDAARKQEEPVDDGKLVEAMFDFLPDSSSDAPTPHGGRETSVFNDLPHAQNVNQDDIIAPIHISEDEEDLSEFKFQKFAATYFQGNVNHQYAKKALKHPLLPLHTQGDQLAAQALWITILRFTGDMPEPKYHTMDRMDTTSVMSKVTATLGRNFIRSKEFQEAQLMGLDPDAFLKQKPRSIRHKLVSLTLKRKNKLGEDVRRRLQDDEYTADSYQSWLQSRPTSNLEKLHFIIGHGILRAELRDEIYCQICKQLTNNPLKSSHARGWILLSLCVGCFAPSEKFVNYLRAFIREGPPGYAPYCEERLKRTFNNGTRNQPPSWLELQATKSKKPIMLPITFMDGNTKTLLADSATTARELCNQLSDKISLKDQFGFSLYIALFDKVSSLGSGGDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEIFAPWHEPTHDQVATNLIYQQVVRGVKFGEYRCDKEEDLAMIAAQQYFIEYSTDMSMERLFTLLPNFIPDFCLSGVDKAIERWAALVLQAYKKSYYVKDKIAPLKIKEDIVSYAKYKWPLLFSRFYEAYRNSGPNLPKNDVIIAVNWTGVYVVDDQEQVLLELSFPEITAVSSQKTNKVFTQTFSLSTVRGEEFTFQSPNAEDIRDLVVYFLDGLKKRSKYVIALQDYRAPSDGTSFLSFFKGDLIILEDESCGESVLNNGWCIGRCDRSQERGDFPAETVYVLPTLSKPPQDILALFNIEEAHHGRRLSMASNGGAVEPRDRPHTLMEYALDHFRLPPKRTMSKTLTLSSKRSEELWRYSRDPIKAPLLRKLQSKEEFAEEACFAFAAILKYMGDLPSKRPRMGNEITDHIFDGPLKHEILRDEIYCQLMKQLTDNRNRMSEERGWELMWLATGLFACSQGLLKELLLFLRTRRHPISQDSMHRLQKTIRHGQRKYPPHQVEVEAIQHKTTQIFHKVYFPDDTDEAFEVDSSTRAKDFCNNISQRLSLRTSEGFSLFVKIADKVISVPEGDFFFDFVRHLTDWIKKARPIRDGANPQFTYQVFFMKKLWTNTVPGKDRNADLIFHYHQELPKLLRGYHKCSREEAAKLAALVFRVRFGENKQELQAIPQMLRELIPSDIMKIQSTSEWKRSIVASYNQDGGMTSEDAKVAFLKIVYRWPTFGSAFFEVKQTTEPNYPEMLLIAINKHGVSLIHPVTKDILVTHPFTRISNWSSGNTYFHMTIGNLVRGSKLLCETSLGYKMDDLLTSYISLMLTNMNKNRTIRAN

Target ORF information:

RefSeq Version	NM_001299020.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster crinkled (ck), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299020.1

ORF Insert Sequence:

ATGGTGATCG TAACACGTGG TGACTACATC TGGATAGAGC CCGCATCTGG GCGAGAATTC 
GATGTGGCCA TTGGAGCACG TGTCGTCTCC GCCGAGGGTC GTCGCATCCA GGTGCGCGAT 
GACGATGGCG ACGAGGTGTG GCTGGCACCC GAGCGCCGCA TCAAGGCCAT GCACGCGTCC 
TCCGTTCAGG GTGTGGAGGA CATGATATCC CTGGGTGATC TGCACGAGGC TGGCATCTTG 
CGGAATCTGC TCATCCGCTA CAAGGAGAAT CTGATATACA CTTACACGGG CTCCATACTT 
GTTGCCGTTA ATCCCTACCA GATTCTGCCC ATCTACACAG GTGATCAGAT CAAGCTCTAT 
AAGGAGCGGA AAATCGGTGA GCTACCGCCG CATATCTTTG CAATTGGCGA CAATGCGTAT 
GCGCATATGA AACGATATCG CCAGGATCAG TGTATCGTTA TTTCCGGAGA GTCTGGAGCT 
GGAAAGACGG AGAGCACAAA GTTGATCCTG CAGTATCTGG CTGCGATTAG TGGCAAACAC 
TCATGGATCG AACAGCAAAT CCTTGAAGCG AATCCCATTT TGGAAGCTTT CGGAAATGCC 
AAAACCATTC GAAATGATAA TTCATCGCGT TTTGGCAAAT ACATAGATAT ACACTTTAGC 
GCCAATGGCG TGATCGAGGG AGCCAAAATA GAACAGTACC TGCTGGAGAA GTCGCGAATT 
GTTTCCCAAA ATCATAGCGA ACGAAATTAT CATGTCTTTT ACTGCATTTT GGCCGGACTG 
TCGGCAGATG AGAAGAGTCG TCTCGATCTT GGCATGGCTG CTGATTACAA ATATCTGACT 
GGTGGTAATA GCATAACCTG CGAGGGACGC GATGACGCCG CCGAATTCTC TGACATTCGA 
TCTGCCATGA AGGTTTTGCT CTTCTCCGAT CAGGAAATCT GGGAGATAAT TAAACTCCTT 
GCTGCCCTTC TCCACTGCGG CAACATCAAA TATAAGGCGA CTGTGGTGGA TAATCTGGAT 
GCTACCGAAA TACCGGAACA CATAAATGTG GAGCGTGTGG CCGGGTTACT TGGACTTCCC 
ATTCAGCCGC TAATTGATGC TCTAACACGT CGAACACTTT TTGCTCATGG AGAAACCGTG 
GTGTCAACTC TGTCGCGCGA TCAATCCGTG GATGTGCGCG ATGCCTTTGT GAAAGGCATA 
TACGGACGTA TGTTTGTGCA CATCGTTCGA AAGATCAATA CGGCAATTTT CAAGCCGCGC 
GGCACATCCC GAAATGCCAT CGGAGTTCTC GACATCTTTG GTTTTGAGAA CTTCGATCAG 
AATAGCTTTG AGCAGTTTTG CATCAACTAT GCCAATGAGA ATCTGCAGCA GTTCTTCGTG 
CAGCATATAT TTAAACTTGA GCAGGAAGAG TATAACCACG AGGCCATCAA CTGGCAACAC 
ATTGAGTTCG TAGACAATCA GGATGCGCTC GACTTGATAG CTATTAAGCA GCTCAATATT 
ATGGCTTTAA TCGATGAGGA GGCCCGGTTT CCCAAGGGCA CGGATCAGAC GATGCTGGCC 
AAATTGCACA AAACCCATGG ATCGCACAAG AACTATTTGA AGCCCAAGTC GGATATCAAC 
ACCAGCTTTG GACTGAATCA CTTTGCCGGT GTGGTGTTCT ACGACACACG AGGATTTCTA 
GATAAAAACC GGGACACCTT CAGTCCCGAT CTATTGCATT TGGTTAGCCA AAGTACGAAC 
AAGTTCCTCC GACAAATCTT TGCTCAGGAC ATAGAGATGG GTGCAGAGAC GCGAAAGCGA 
ACACCCACAC TCTCCACACA GTTTCGAAAA TCTCTCGATG CGCTTATGAA GACGCTCAGC 
AGTTGCCAGC CATTCTTTAT TCGTTGTATC AAACCCAACG AGCTGAAGAA GCCAATGATG 
TTTGACCGCG GCTTGTGTTG CCGTCAGCTG CGATATTCCG GCATGATGGA AACGATCCGA 
ATCCGTCGCG CTGGATATCC CATTCGACAT GGATTCAGAG AGTTCGTCGA GCGTTATCGC 
TTCCTGATAC CGGGAGTTCC ACCAGCTCAT CGCACGGATT GCCAGGCGGC GACTTCGAGG 
ATTTGTGCCG TGGTTTTGGG CAAGTCCGAC TATCAGCTGG GCCACACCAA GGTCTTTCTC 
AAGGACGCCC ACGATCTGTT TCTGGAGCAG GAGAGGGATC GCGTGCTGAC GCGAAAAATC 
CTCATTCTGC AGCGCAGCAT TCGTGGTTGG GTGTACCGAC GAAGGTTTCT ACGCCTGAGA 
GCTGCTGCTA TCACTGTGCA GCGATTCTGG AAGGGCTATG CTCAACGTAA ACGATACAGG 
AACATGCGAG TGGGCTACAT GCGTTTGCAA GCGTTGATCC GTTCGAGAGT TCTGTCACAT 
CGTTTCCGCC ATCTGAGGGG CCACATTGTC GGGTTGCAAG CCCATGCCCG AGGATATTTG 
GTGAGGAGAG AGTATGGTCA CAAGATGTGG GCCGTCATTA AGATCCAGTC TCATGTGCGG 
CGTATGATTG CCATGCGGCG CTACCGAAAA CTTCGATTGG AGCACAAACA GTTTGCCGAA 
GTTCTTCAGC TGCGCAAACT GGAGGAGCAG GAACTGCTGC ATCGTGGCAA TAAGCATGCC 
CGAGAAATTG CCGAGCAGCA TTACCGAGAT CGATTGCACG AGCTGGAGCG TCGGGAAATT 
CAGGAGCAAT TGGAGAATCG TCGTCGAGTG GAGGTCAATA TGAATATTAT CAATGATGCA 
GCTCGCAAAC AGGAGGAGCC GGTGGACGAT GGCAAACTGG TGGAGGCCAT GTTTGACTTC 
CTTCCCGATT CCAGTAGCGA TGCCCCTACT CCGCATGGTG GACGTGAAAC GTCCGTGTTC 
AACGATCTGC CTCACGCGCA AAACGTCAAC CAGGACGATA TCATTGCACC CATACACATA 
TCCGAGGATG AGGAGGATCT ATCGGAATTC AAGTTCCAGA AATTCGCTGC CACCTACTTC 
CAAGGAAATG TCAATCATCA GTATGCTAAG AAAGCTTTGA AGCATCCCCT ACTTCCGCTC 
CATACGCAAG GCGATCAACT TGCTGCACAG GCATTGTGGA TAACCATACT GAGGTTTACA 
GGTGATATGC CGGAGCCGAA GTATCACACA ATGGATCGTA TGGACACCAC TTCGGTCATG 
TCCAAGGTCA CTGCCACCTT GGGGCGCAAT TTCATCAGGA GTAAGGAGTT CCAAGAGGCT 
CAGCTTATGG GCCTGGATCC AGATGCATTC CTTAAGCAAA AGCCACGATC GATCCGCCAC 
AAACTCGTAT CCCTGACGTT GAAGCGAAAG AACAAATTGG GCGAGGATGT GCGTCGCCGA 
CTGCAAGATG ATGAATACAC GGCGGATAGC TACCAATCGT GGCTACAATC CCGTCCAACC 
TCCAACCTTG AAAAGCTGCA TTTTATCATT GGCCATGGTA TCCTGCGTGC CGAGCTGCGT 
GACGAAATAT ACTGCCAGAT ATGCAAGCAG CTGACGAACA ATCCATTGAA ATCATCACAT 
GCCAGAGGTT GGATTCTGTT ATCCCTGTGC GTCGGATGTT TTGCTCCGTC GGAAAAGTTT 
GTTAACTACT TGAGGGCCTT TATCCGTGAG GGTCCACCTG GATATGCACC GTACTGCGAG 
GAGCGACTGA AGCGTACTTT CAACAATGGA ACTCGCAATC AACCGCCTTC GTGGCTGGAG 
TTGCAGGCCA CCAAATCCAA AAAGCCCATC ATGCTGCCCA TTACCTTTAT GGATGGAAAT 
ACAAAGACTC TGTTGGCTGA TTCCGCCACC ACGGCCCGAG AACTATGCAA TCAACTGTCC 
GACAAGATAA GTCTCAAAGA TCAGTTTGGT TTTTCCCTGT ACATAGCTCT GTTCGACAAA 
GTTAGTTCTT TGGGAAGTGG CGGGGACCAC GTGATGGACG CCATCTCACA ATGTGAGCAG 
TATGCAAAGG AGCAGGGCGC CCAGGAGCGG AATGCCCCGT GGCGTCTGTT TTTCCGCAAG 
GAAATCTTTG CACCTTGGCA CGAACCCACA CACGATCAGG TGGCCACCAA TCTTATTTAC 
CAGCAGGTGG TGAGAGGCGT TAAGTTTGGC GAATATCGCT GCGACAAGGA GGAAGACTTG 
GCCATGATAG CTGCGCAGCA ATATTTCATC GAATACTCAA CCGATATGTC AATGGAGCGG 
CTGTTTACCC TTCTGCCCAA CTTTATACCC GACTTTTGTC TCAGCGGAGT GGACAAGGCC 
ATTGAACGTT GGGCTGCTTT GGTTCTGCAG GCCTATAAAA AATCGTACTA TGTCAAGGAC 
AAGATCGCAC CCTTGAAAAT TAAGGAGGAT ATAGTTAGCT ACGCCAAGTA CAAGTGGCCT 
CTGCTCTTTT CTCGTTTCTA CGAAGCGTAT CGAAACTCCG GTCCGAATCT GCCCAAAAAC 
GATGTCATCA TTGCGGTGAA CTGGACCGGC GTGTATGTGG TTGATGATCA GGAGCAGGTG 
CTGTTGGAAC TGAGCTTCCC CGAGATTACT GCCGTGTCCA GTCAGAAGAC CAACAAGGTC 
TTCACCCAGA CTTTCAGCCT TTCCACTGTT CGAGGCGAGG AGTTCACCTT CCAGAGCCCC 
AATGCGGAGG ATATCCGTGA TCTGGTTGTC TACTTCTTAG ATGGCCTGAA GAAACGATCG 
AAATATGTCA TAGCCTTGCA GGACTATCGT GCGCCTTCGG ATGGAACAAG TTTTCTGTCT 
TTCTTTAAGG GAGATCTGAT CATACTAGAG GACGAATCCT GTGGAGAATC AGTACTGAAC 
AACGGCTGGT GCATAGGACG CTGCGATCGT TCCCAGGAGC GAGGAGACTT CCCCGCCGAA 
ACCGTGTACG TGCTGCCCAC CCTTAGCAAA CCGCCGCAGG ATATTTTGGC CCTGTTCAAC 
ATCGAGGAGG CGCATCATGG ACGACGTCTA AGCATGGCTT CCAATGGTGG AGCTGTGGAA 
CCAAGGGATC GACCTCATAC GCTGATGGAG TATGCCCTGG ATCACTTCCG TTTGCCACCG 
AAACGAACAA TGTCGAAAAC ACTCACTCTG AGTTCAAAAC GGAGTGAGGA ATTGTGGCGC 
TACTCCCGGG ATCCCATTAA GGCACCGCTG CTGCGCAAGT TGCAAAGCAA GGAAGAGTTT 
GCCGAGGAGG CCTGCTTCGC GTTTGCCGCC ATACTGAAAT ATATGGGCGA TCTGCCCTCC 
AAGCGTCCGC GGATGGGCAA CGAAATAACC GATCACATAT TTGATGGACC TCTGAAGCAC 
GAAATTCTGC GCGACGAGAT CTACTGCCAA CTGATGAAGC AGCTAACGGA CAATCGCAAC 
CGAATGTCCG AGGAGAGGGG CTGGGAGCTG ATGTGGTTGG CCACGGGACT GTTTGCCTGT 
TCACAGGGTT TGCTCAAGGA ATTGCTATTA TTCCTGCGCA CCAGGCGTCA TCCCATTTCA 
CAGGACTCAA TGCATCGACT ACAGAAAACT ATACGCCATG GACAACGCAA ATATCCGCCG 
CATCAGGTGG AGGTGGAAGC CATACAGCAT AAAACCACAC AGATCTTCCA CAAGGTCTAC 
TTCCCGGACG ACACAGACGA GGCCTTCGAA GTGGACAGCT CCACTCGAGC GAAGGATTTC 
TGCAACAACA TCTCGCAGCG CCTTTCGCTG CGCACCAGCG AGGGCTTCTC CCTGTTTGTA 
AAGATCGCGG ATAAGGTCAT CTCTGTTCCC GAAGGTGATT TCTTCTTCGA TTTCGTACGC 
CATTTGACCG ACTGGATTAA AAAAGCGCGT CCCATACGAG ATGGAGCGAA TCCGCAGTTC 
ACTTACCAGG TCTTCTTCAT GAAGAAACTG TGGACGAACA CAGTGCCAGG CAAGGATCGG 
AATGCCGATC TTATATTCCA CTATCATCAG GAGTTGCCCA AGCTGTTGCG TGGCTATCAC 
AAGTGTTCCA GGGAGGAGGC GGCTAAGCTG GCCGCTCTAG TCTTCCGTGT GCGCTTTGGT 
GAAAATAAAC AAGAACTGCA GGCCATACCA CAAATGCTGA GGGAACTTAT TCCATCGGAT 
ATTATGAAAA TACAGAGCAC TAGCGAATGG AAACGATCGA TTGTTGCATC TTATAACCAG 
GATGGGGGCA TGACTTCCGA AGACGCGAAG GTGGCCTTCT TGAAGATTGT ATATAGATGG 
CCAACTTTTG GCTCCGCTTT CTTTGAGGTG AAGCAAACCA CCGAACCGAA TTATCCCGAA 
ATGCTATTGA TAGCCATTAA CAAACACGGC GTGAGTCTCA TACATCCAGT GACCAAGGAC 
ATTTTGGTTA CGCATCCATT TACACGCATC TCGAATTGGT CATCGGGCAA TACATATTTC 
CACATGACCA TCGGAAATCT GGTTAGGGGC TCGAAATTGT TATGTGAAAC GTCGCTGGGC 
TACAAGATGG ACGATCTGCT GACTTCATAT ATCTCGTTGA TGTTGACCAA TATGAACAAA 
AATCGAACCA TTCGAGCCAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ck ( NM_165099.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723895.1 Drosophila melanogaster crinkled (ck), transcript variant A, mRNA.

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CloneID	OFa15890
Clone ID Related Accession (Same CDS sequence)	NM_001299020.1 , NM_165099.3 , NM_001299021.1 , NM_078847.4
Accession Version	NM_165099.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	crinkled, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_165099.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGATCG TAACACGTGG TGACTACATC TGGATAGAGC CCGCATCTGG GCGAGAATTC 
GATGTGGCCA TTGGAGCACG TGTCGTCTCC GCCGAGGGTC GTCGCATCCA GGTGCGCGAT 
GACGATGGCG ACGAGGTGTG GCTGGCACCC GAGCGCCGCA TCAAGGCCAT GCACGCGTCC 
TCCGTTCAGG GTGTGGAGGA CATGATATCC CTGGGTGATC TGCACGAGGC TGGCATCTTG 
CGGAATCTGC TCATCCGCTA CAAGGAGAAT CTGATATACA CTTACACGGG CTCCATACTT 
GTTGCCGTTA ATCCCTACCA GATTCTGCCC ATCTACACAG GTGATCAGAT CAAGCTCTAT 
AAGGAGCGGA AAATCGGTGA GCTACCGCCG CATATCTTTG CAATTGGCGA CAATGCGTAT 
GCGCATATGA AACGATATCG CCAGGATCAG TGTATCGTTA TTTCCGGAGA GTCTGGAGCT 
GGAAAGACGG AGAGCACAAA GTTGATCCTG CAGTATCTGG CTGCGATTAG TGGCAAACAC 
TCATGGATCG AACAGCAAAT CCTTGAAGCG AATCCCATTT TGGAAGCTTT CGGAAATGCC 
AAAACCATTC GAAATGATAA TTCATCGCGT TTTGGCAAAT ACATAGATAT ACACTTTAGC 
GCCAATGGCG TGATCGAGGG AGCCAAAATA GAACAGTACC TGCTGGAGAA GTCGCGAATT 
GTTTCCCAAA ATCATAGCGA ACGAAATTAT CATGTCTTTT ACTGCATTTT GGCCGGACTG 
TCGGCAGATG AGAAGAGTCG TCTCGATCTT GGCATGGCTG CTGATTACAA ATATCTGACT 
GGTGGTAATA GCATAACCTG CGAGGGACGC GATGACGCCG CCGAATTCTC TGACATTCGA 
TCTGCCATGA AGGTTTTGCT CTTCTCCGAT CAGGAAATCT GGGAGATAAT TAAACTCCTT 
GCTGCCCTTC TCCACTGCGG CAACATCAAA TATAAGGCGA CTGTGGTGGA TAATCTGGAT 
GCTACCGAAA TACCGGAACA CATAAATGTG GAGCGTGTGG CCGGGTTACT TGGACTTCCC 
ATTCAGCCGC TAATTGATGC TCTAACACGT CGAACACTTT TTGCTCATGG AGAAACCGTG 
GTGTCAACTC TGTCGCGCGA TCAATCCGTG GATGTGCGCG ATGCCTTTGT GAAAGGCATA 
TACGGACGTA TGTTTGTGCA CATCGTTCGA AAGATCAATA CGGCAATTTT CAAGCCGCGC 
GGCACATCCC GAAATGCCAT CGGAGTTCTC GACATCTTTG GTTTTGAGAA CTTCGATCAG 
AATAGCTTTG AGCAGTTTTG CATCAACTAT GCCAATGAGA ATCTGCAGCA GTTCTTCGTG 
CAGCATATAT TTAAACTTGA GCAGGAAGAG TATAACCACG AGGCCATCAA CTGGCAACAC 
ATTGAGTTCG TAGACAATCA GGATGCGCTC GACTTGATAG CTATTAAGCA GCTCAATATT 
ATGGCTTTAA TCGATGAGGA GGCCCGGTTT CCCAAGGGCA CGGATCAGAC GATGCTGGCC 
AAATTGCACA AAACCCATGG ATCGCACAAG AACTATTTGA AGCCCAAGTC GGATATCAAC 
ACCAGCTTTG GACTGAATCA CTTTGCCGGT GTGGTGTTCT ACGACACACG AGGATTTCTA 
GATAAAAACC GGGACACCTT CAGTCCCGAT CTATTGCATT TGGTTAGCCA AAGTACGAAC 
AAGTTCCTCC GACAAATCTT TGCTCAGGAC ATAGAGATGG GTGCAGAGAC GCGAAAGCGA 
ACACCCACAC TCTCCACACA GTTTCGAAAA TCTCTCGATG CGCTTATGAA GACGCTCAGC 
AGTTGCCAGC CATTCTTTAT TCGTTGTATC AAACCCAACG AGCTGAAGAA GCCAATGATG 
TTTGACCGCG GCTTGTGTTG CCGTCAGCTG CGATATTCCG GCATGATGGA AACGATCCGA 
ATCCGTCGCG CTGGATATCC CATTCGACAT GGATTCAGAG AGTTCGTCGA GCGTTATCGC 
TTCCTGATAC CGGGAGTTCC ACCAGCTCAT CGCACGGATT GCCAGGCGGC GACTTCGAGG 
ATTTGTGCCG TGGTTTTGGG CAAGTCCGAC TATCAGCTGG GCCACACCAA GGTCTTTCTC 
AAGGACGCCC ACGATCTGTT TCTGGAGCAG GAGAGGGATC GCGTGCTGAC GCGAAAAATC 
CTCATTCTGC AGCGCAGCAT TCGTGGTTGG GTGTACCGAC GAAGGTTTCT ACGCCTGAGA 
GCTGCTGCTA TCACTGTGCA GCGATTCTGG AAGGGCTATG CTCAACGTAA ACGATACAGG 
AACATGCGAG TGGGCTACAT GCGTTTGCAA GCGTTGATCC GTTCGAGAGT TCTGTCACAT 
CGTTTCCGCC ATCTGAGGGG CCACATTGTC GGGTTGCAAG CCCATGCCCG AGGATATTTG 
GTGAGGAGAG AGTATGGTCA CAAGATGTGG GCCGTCATTA AGATCCAGTC TCATGTGCGG 
CGTATGATTG CCATGCGGCG CTACCGAAAA CTTCGATTGG AGCACAAACA GTTTGCCGAA 
GTTCTTCAGC TGCGCAAACT GGAGGAGCAG GAACTGCTGC ATCGTGGCAA TAAGCATGCC 
CGAGAAATTG CCGAGCAGCA TTACCGAGAT CGATTGCACG AGCTGGAGCG TCGGGAAATT 
CAGGAGCAAT TGGAGAATCG TCGTCGAGTG GAGGTCAATA TGAATATTAT CAATGATGCA 
GCTCGCAAAC AGGAGGAGCC GGTGGACGAT GGCAAACTGG TGGAGGCCAT GTTTGACTTC 
CTTCCCGATT CCAGTAGCGA TGCCCCTACT CCGCATGGTG GACGTGAAAC GTCCGTGTTC 
AACGATCTGC CTCACGCGCA AAACGTCAAC CAGGACGATA TCATTGCACC CATACACATA 
TCCGAGGATG AGGAGGATCT ATCGGAATTC AAGTTCCAGA AATTCGCTGC CACCTACTTC 
CAAGGAAATG TCAATCATCA GTATGCTAAG AAAGCTTTGA AGCATCCCCT ACTTCCGCTC 
CATACGCAAG GCGATCAACT TGCTGCACAG GCATTGTGGA TAACCATACT GAGGTTTACA 
GGTGATATGC CGGAGCCGAA GTATCACACA ATGGATCGTA TGGACACCAC TTCGGTCATG 
TCCAAGGTCA CTGCCACCTT GGGGCGCAAT TTCATCAGGA GTAAGGAGTT CCAAGAGGCT 
CAGCTTATGG GCCTGGATCC AGATGCATTC CTTAAGCAAA AGCCACGATC GATCCGCCAC 
AAACTCGTAT CCCTGACGTT GAAGCGAAAG AACAAATTGG GCGAGGATGT GCGTCGCCGA 
CTGCAAGATG ATGAATACAC GGCGGATAGC TACCAATCGT GGCTACAATC CCGTCCAACC 
TCCAACCTTG AAAAGCTGCA TTTTATCATT GGCCATGGTA TCCTGCGTGC CGAGCTGCGT 
GACGAAATAT ACTGCCAGAT ATGCAAGCAG CTGACGAACA ATCCATTGAA ATCATCACAT 
GCCAGAGGTT GGATTCTGTT ATCCCTGTGC GTCGGATGTT TTGCTCCGTC GGAAAAGTTT 
GTTAACTACT TGAGGGCCTT TATCCGTGAG GGTCCACCTG GATATGCACC GTACTGCGAG 
GAGCGACTGA AGCGTACTTT CAACAATGGA ACTCGCAATC AACCGCCTTC GTGGCTGGAG 
TTGCAGGCCA CCAAATCCAA AAAGCCCATC ATGCTGCCCA TTACCTTTAT GGATGGAAAT 
ACAAAGACTC TGTTGGCTGA TTCCGCCACC ACGGCCCGAG AACTATGCAA TCAACTGTCC 
GACAAGATAA GTCTCAAAGA TCAGTTTGGT TTTTCCCTGT ACATAGCTCT GTTCGACAAA 
GTTAGTTCTT TGGGAAGTGG CGGGGACCAC GTGATGGACG CCATCTCACA ATGTGAGCAG 
TATGCAAAGG AGCAGGGCGC CCAGGAGCGG AATGCCCCGT GGCGTCTGTT TTTCCGCAAG 
GAAATCTTTG CACCTTGGCA CGAACCCACA CACGATCAGG TGGCCACCAA TCTTATTTAC 
CAGCAGGTGG TGAGAGGCGT TAAGTTTGGC GAATATCGCT GCGACAAGGA GGAAGACTTG 
GCCATGATAG CTGCGCAGCA ATATTTCATC GAATACTCAA CCGATATGTC AATGGAGCGG 
CTGTTTACCC TTCTGCCCAA CTTTATACCC GACTTTTGTC TCAGCGGAGT GGACAAGGCC 
ATTGAACGTT GGGCTGCTTT GGTTCTGCAG GCCTATAAAA AATCGTACTA TGTCAAGGAC 
AAGATCGCAC CCTTGAAAAT TAAGGAGGAT ATAGTTAGCT ACGCCAAGTA CAAGTGGCCT 
CTGCTCTTTT CTCGTTTCTA CGAAGCGTAT CGAAACTCCG GTCCGAATCT GCCCAAAAAC 
GATGTCATCA TTGCGGTGAA CTGGACCGGC GTGTATGTGG TTGATGATCA GGAGCAGGTG 
CTGTTGGAAC TGAGCTTCCC CGAGATTACT GCCGTGTCCA GTCAGAAGAC CAACAAGGTC 
TTCACCCAGA CTTTCAGCCT TTCCACTGTT CGAGGCGAGG AGTTCACCTT CCAGAGCCCC 
AATGCGGAGG ATATCCGTGA TCTGGTTGTC TACTTCTTAG ATGGCCTGAA GAAACGATCG 
AAATATGTCA TAGCCTTGCA GGACTATCGT GCGCCTTCGG ATGGAACAAG TTTTCTGTCT 
TTCTTTAAGG GAGATCTGAT CATACTAGAG GACGAATCCT GTGGAGAATC AGTACTGAAC 
AACGGCTGGT GCATAGGACG CTGCGATCGT TCCCAGGAGC GAGGAGACTT CCCCGCCGAA 
ACCGTGTACG TGCTGCCCAC CCTTAGCAAA CCGCCGCAGG ATATTTTGGC CCTGTTCAAC 
ATCGAGGAGG CGCATCATGG ACGACGTCTA AGCATGGCTT CCAATGGTGG AGCTGTGGAA 
CCAAGGGATC GACCTCATAC GCTGATGGAG TATGCCCTGG ATCACTTCCG TTTGCCACCG 
AAACGAACAA TGTCGAAAAC ACTCACTCTG AGTTCAAAAC GGAGTGAGGA ATTGTGGCGC 
TACTCCCGGG ATCCCATTAA GGCACCGCTG CTGCGCAAGT TGCAAAGCAA GGAAGAGTTT 
GCCGAGGAGG CCTGCTTCGC GTTTGCCGCC ATACTGAAAT ATATGGGCGA TCTGCCCTCC 
AAGCGTCCGC GGATGGGCAA CGAAATAACC GATCACATAT TTGATGGACC TCTGAAGCAC 
GAAATTCTGC GCGACGAGAT CTACTGCCAA CTGATGAAGC AGCTAACGGA CAATCGCAAC 
CGAATGTCCG AGGAGAGGGG CTGGGAGCTG ATGTGGTTGG CCACGGGACT GTTTGCCTGT 
TCACAGGGTT TGCTCAAGGA ATTGCTATTA TTCCTGCGCA CCAGGCGTCA TCCCATTTCA 
CAGGACTCAA TGCATCGACT ACAGAAAACT ATACGCCATG GACAACGCAA ATATCCGCCG 
CATCAGGTGG AGGTGGAAGC CATACAGCAT AAAACCACAC AGATCTTCCA CAAGGTCTAC 
TTCCCGGACG ACACAGACGA GGCCTTCGAA GTGGACAGCT CCACTCGAGC GAAGGATTTC 
TGCAACAACA TCTCGCAGCG CCTTTCGCTG CGCACCAGCG AGGGCTTCTC CCTGTTTGTA 
AAGATCGCGG ATAAGGTCAT CTCTGTTCCC GAAGGTGATT TCTTCTTCGA TTTCGTACGC 
CATTTGACCG ACTGGATTAA AAAAGCGCGT CCCATACGAG ATGGAGCGAA TCCGCAGTTC 
ACTTACCAGG TCTTCTTCAT GAAGAAACTG TGGACGAACA CAGTGCCAGG CAAGGATCGG 
AATGCCGATC TTATATTCCA CTATCATCAG GAGTTGCCCA AGCTGTTGCG TGGCTATCAC 
AAGTGTTCCA GGGAGGAGGC GGCTAAGCTG GCCGCTCTAG TCTTCCGTGT GCGCTTTGGT 
GAAAATAAAC AAGAACTGCA GGCCATACCA CAAATGCTGA GGGAACTTAT TCCATCGGAT 
ATTATGAAAA TACAGAGCAC TAGCGAATGG AAACGATCGA TTGTTGCATC TTATAACCAG 
GATGGGGGCA TGACTTCCGA AGACGCGAAG GTGGCCTTCT TGAAGATTGT ATATAGATGG 
CCAACTTTTG GCTCCGCTTT CTTTGAGGTG AAGCAAACCA CCGAACCGAA TTATCCCGAA 
ATGCTATTGA TAGCCATTAA CAAACACGGC GTGAGTCTCA TACATCCAGT GACCAAGGAC 
ATTTTGGTTA CGCATCCATT TACACGCATC TCGAATTGGT CATCGGGCAA TACATATTTC 
CACATGACCA TCGGAAATCT GGTTAGGGGC TCGAAATTGT TATGTGAAAC GTCGCTGGGC 
TACAAGATGG ACGATCTGCT GACTTCATAT ATCTCGTTGA TGTTGACCAA TATGAACAAA 
AATCGAACCA TTCGAGCCAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723895.1
CDS	287..6790
Translation	MVIVTRGDYIWIEPASGREFDVAIGARVVSAEGRRIQVRDDDGDEVWLAPERRIKAMHASSVQGVEDMISLGDLHEAGILRNLLIRYKENLIYTYTGSILVAVNPYQILPIYTGDQIKLYKERKIGELPPHIFAIGDNAYAHMKRYRQDQCIVISGESGAGKTESTKLILQYLAAISGKHSWIEQQILEANPILEAFGNAKTIRNDNSSRFGKYIDIHFSANGVIEGAKIEQYLLEKSRIVSQNHSERNYHVFYCILAGLSADEKSRLDLGMAADYKYLTGGNSITCEGRDDAAEFSDIRSAMKVLLFSDQEIWEIIKLLAALLHCGNIKYKATVVDNLDATEIPEHINVERVAGLLGLPIQPLIDALTRRTLFAHGETVVSTLSRDQSVDVRDAFVKGIYGRMFVHIVRKINTAIFKPRGTSRNAIGVLDIFGFENFDQNSFEQFCINYANENLQQFFVQHIFKLEQEEYNHEAINWQHIEFVDNQDALDLIAIKQLNIMALIDEEARFPKGTDQTMLAKLHKTHGSHKNYLKPKSDINTSFGLNHFAGVVFYDTRGFLDKNRDTFSPDLLHLVSQSTNKFLRQIFAQDIEMGAETRKRTPTLSTQFRKSLDALMKTLSSCQPFFIRCIKPNELKKPMMFDRGLCCRQLRYSGMMETIRIRRAGYPIRHGFREFVERYRFLIPGVPPAHRTDCQAATSRICAVVLGKSDYQLGHTKVFLKDAHDLFLEQERDRVLTRKILILQRSIRGWVYRRRFLRLRAAAITVQRFWKGYAQRKRYRNMRVGYMRLQALIRSRVLSHRFRHLRGHIVGLQAHARGYLVRREYGHKMWAVIKIQSHVRRMIAMRRYRKLRLEHKQFAEVLQLRKLEEQELLHRGNKHAREIAEQHYRDRLHELERREIQEQLENRRRVEVNMNIINDAARKQEEPVDDGKLVEAMFDFLPDSSSDAPTPHGGRETSVFNDLPHAQNVNQDDIIAPIHISEDEEDLSEFKFQKFAATYFQGNVNHQYAKKALKHPLLPLHTQGDQLAAQALWITILRFTGDMPEPKYHTMDRMDTTSVMSKVTATLGRNFIRSKEFQEAQLMGLDPDAFLKQKPRSIRHKLVSLTLKRKNKLGEDVRRRLQDDEYTADSYQSWLQSRPTSNLEKLHFIIGHGILRAELRDEIYCQICKQLTNNPLKSSHARGWILLSLCVGCFAPSEKFVNYLRAFIREGPPGYAPYCEERLKRTFNNGTRNQPPSWLELQATKSKKPIMLPITFMDGNTKTLLADSATTARELCNQLSDKISLKDQFGFSLYIALFDKVSSLGSGGDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEIFAPWHEPTHDQVATNLIYQQVVRGVKFGEYRCDKEEDLAMIAAQQYFIEYSTDMSMERLFTLLPNFIPDFCLSGVDKAIERWAALVLQAYKKSYYVKDKIAPLKIKEDIVSYAKYKWPLLFSRFYEAYRNSGPNLPKNDVIIAVNWTGVYVVDDQEQVLLELSFPEITAVSSQKTNKVFTQTFSLSTVRGEEFTFQSPNAEDIRDLVVYFLDGLKKRSKYVIALQDYRAPSDGTSFLSFFKGDLIILEDESCGESVLNNGWCIGRCDRSQERGDFPAETVYVLPTLSKPPQDILALFNIEEAHHGRRLSMASNGGAVEPRDRPHTLMEYALDHFRLPPKRTMSKTLTLSSKRSEELWRYSRDPIKAPLLRKLQSKEEFAEEACFAFAAILKYMGDLPSKRPRMGNEITDHIFDGPLKHEILRDEIYCQLMKQLTDNRNRMSEERGWELMWLATGLFACSQGLLKELLLFLRTRRHPISQDSMHRLQKTIRHGQRKYPPHQVEVEAIQHKTTQIFHKVYFPDDTDEAFEVDSSTRAKDFCNNISQRLSLRTSEGFSLFVKIADKVISVPEGDFFFDFVRHLTDWIKKARPIRDGANPQFTYQVFFMKKLWTNTVPGKDRNADLIFHYHQELPKLLRGYHKCSREEAAKLAALVFRVRFGENKQELQAIPQMLRELIPSDIMKIQSTSEWKRSIVASYNQDGGMTSEDAKVAFLKIVYRWPTFGSAFFEVKQTTEPNYPEMLLIAINKHGVSLIHPVTKDILVTHPFTRISNWSSGNTYFHMTIGNLVRGSKLLCETSLGYKMDDLLTSYISLMLTNMNKNRTIRAN

Target ORF information:

RefSeq Version	NM_165099.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster crinkled (ck), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165099.3

ORF Insert Sequence:

ATGGTGATCG TAACACGTGG TGACTACATC TGGATAGAGC CCGCATCTGG GCGAGAATTC 
GATGTGGCCA TTGGAGCACG TGTCGTCTCC GCCGAGGGTC GTCGCATCCA GGTGCGCGAT 
GACGATGGCG ACGAGGTGTG GCTGGCACCC GAGCGCCGCA TCAAGGCCAT GCACGCGTCC 
TCCGTTCAGG GTGTGGAGGA CATGATATCC CTGGGTGATC TGCACGAGGC TGGCATCTTG 
CGGAATCTGC TCATCCGCTA CAAGGAGAAT CTGATATACA CTTACACGGG CTCCATACTT 
GTTGCCGTTA ATCCCTACCA GATTCTGCCC ATCTACACAG GTGATCAGAT CAAGCTCTAT 
AAGGAGCGGA AAATCGGTGA GCTACCGCCG CATATCTTTG CAATTGGCGA CAATGCGTAT 
GCGCATATGA AACGATATCG CCAGGATCAG TGTATCGTTA TTTCCGGAGA GTCTGGAGCT 
GGAAAGACGG AGAGCACAAA GTTGATCCTG CAGTATCTGG CTGCGATTAG TGGCAAACAC 
TCATGGATCG AACAGCAAAT CCTTGAAGCG AATCCCATTT TGGAAGCTTT CGGAAATGCC 
AAAACCATTC GAAATGATAA TTCATCGCGT TTTGGCAAAT ACATAGATAT ACACTTTAGC 
GCCAATGGCG TGATCGAGGG AGCCAAAATA GAACAGTACC TGCTGGAGAA GTCGCGAATT 
GTTTCCCAAA ATCATAGCGA ACGAAATTAT CATGTCTTTT ACTGCATTTT GGCCGGACTG 
TCGGCAGATG AGAAGAGTCG TCTCGATCTT GGCATGGCTG CTGATTACAA ATATCTGACT 
GGTGGTAATA GCATAACCTG CGAGGGACGC GATGACGCCG CCGAATTCTC TGACATTCGA 
TCTGCCATGA AGGTTTTGCT CTTCTCCGAT CAGGAAATCT GGGAGATAAT TAAACTCCTT 
GCTGCCCTTC TCCACTGCGG CAACATCAAA TATAAGGCGA CTGTGGTGGA TAATCTGGAT 
GCTACCGAAA TACCGGAACA CATAAATGTG GAGCGTGTGG CCGGGTTACT TGGACTTCCC 
ATTCAGCCGC TAATTGATGC TCTAACACGT CGAACACTTT TTGCTCATGG AGAAACCGTG 
GTGTCAACTC TGTCGCGCGA TCAATCCGTG GATGTGCGCG ATGCCTTTGT GAAAGGCATA 
TACGGACGTA TGTTTGTGCA CATCGTTCGA AAGATCAATA CGGCAATTTT CAAGCCGCGC 
GGCACATCCC GAAATGCCAT CGGAGTTCTC GACATCTTTG GTTTTGAGAA CTTCGATCAG 
AATAGCTTTG AGCAGTTTTG CATCAACTAT GCCAATGAGA ATCTGCAGCA GTTCTTCGTG 
CAGCATATAT TTAAACTTGA GCAGGAAGAG TATAACCACG AGGCCATCAA CTGGCAACAC 
ATTGAGTTCG TAGACAATCA GGATGCGCTC GACTTGATAG CTATTAAGCA GCTCAATATT 
ATGGCTTTAA TCGATGAGGA GGCCCGGTTT CCCAAGGGCA CGGATCAGAC GATGCTGGCC 
AAATTGCACA AAACCCATGG ATCGCACAAG AACTATTTGA AGCCCAAGTC GGATATCAAC 
ACCAGCTTTG GACTGAATCA CTTTGCCGGT GTGGTGTTCT ACGACACACG AGGATTTCTA 
GATAAAAACC GGGACACCTT CAGTCCCGAT CTATTGCATT TGGTTAGCCA AAGTACGAAC 
AAGTTCCTCC GACAAATCTT TGCTCAGGAC ATAGAGATGG GTGCAGAGAC GCGAAAGCGA 
ACACCCACAC TCTCCACACA GTTTCGAAAA TCTCTCGATG CGCTTATGAA GACGCTCAGC 
AGTTGCCAGC CATTCTTTAT TCGTTGTATC AAACCCAACG AGCTGAAGAA GCCAATGATG 
TTTGACCGCG GCTTGTGTTG CCGTCAGCTG CGATATTCCG GCATGATGGA AACGATCCGA 
ATCCGTCGCG CTGGATATCC CATTCGACAT GGATTCAGAG AGTTCGTCGA GCGTTATCGC 
TTCCTGATAC CGGGAGTTCC ACCAGCTCAT CGCACGGATT GCCAGGCGGC GACTTCGAGG 
ATTTGTGCCG TGGTTTTGGG CAAGTCCGAC TATCAGCTGG GCCACACCAA GGTCTTTCTC 
AAGGACGCCC ACGATCTGTT TCTGGAGCAG GAGAGGGATC GCGTGCTGAC GCGAAAAATC 
CTCATTCTGC AGCGCAGCAT TCGTGGTTGG GTGTACCGAC GAAGGTTTCT ACGCCTGAGA 
GCTGCTGCTA TCACTGTGCA GCGATTCTGG AAGGGCTATG CTCAACGTAA ACGATACAGG 
AACATGCGAG TGGGCTACAT GCGTTTGCAA GCGTTGATCC GTTCGAGAGT TCTGTCACAT 
CGTTTCCGCC ATCTGAGGGG CCACATTGTC GGGTTGCAAG CCCATGCCCG AGGATATTTG 
GTGAGGAGAG AGTATGGTCA CAAGATGTGG GCCGTCATTA AGATCCAGTC TCATGTGCGG 
CGTATGATTG CCATGCGGCG CTACCGAAAA CTTCGATTGG AGCACAAACA GTTTGCCGAA 
GTTCTTCAGC TGCGCAAACT GGAGGAGCAG GAACTGCTGC ATCGTGGCAA TAAGCATGCC 
CGAGAAATTG CCGAGCAGCA TTACCGAGAT CGATTGCACG AGCTGGAGCG TCGGGAAATT 
CAGGAGCAAT TGGAGAATCG TCGTCGAGTG GAGGTCAATA TGAATATTAT CAATGATGCA 
GCTCGCAAAC AGGAGGAGCC GGTGGACGAT GGCAAACTGG TGGAGGCCAT GTTTGACTTC 
CTTCCCGATT CCAGTAGCGA TGCCCCTACT CCGCATGGTG GACGTGAAAC GTCCGTGTTC 
AACGATCTGC CTCACGCGCA AAACGTCAAC CAGGACGATA TCATTGCACC CATACACATA 
TCCGAGGATG AGGAGGATCT ATCGGAATTC AAGTTCCAGA AATTCGCTGC CACCTACTTC 
CAAGGAAATG TCAATCATCA GTATGCTAAG AAAGCTTTGA AGCATCCCCT ACTTCCGCTC 
CATACGCAAG GCGATCAACT TGCTGCACAG GCATTGTGGA TAACCATACT GAGGTTTACA 
GGTGATATGC CGGAGCCGAA GTATCACACA ATGGATCGTA TGGACACCAC TTCGGTCATG 
TCCAAGGTCA CTGCCACCTT GGGGCGCAAT TTCATCAGGA GTAAGGAGTT CCAAGAGGCT 
CAGCTTATGG GCCTGGATCC AGATGCATTC CTTAAGCAAA AGCCACGATC GATCCGCCAC 
AAACTCGTAT CCCTGACGTT GAAGCGAAAG AACAAATTGG GCGAGGATGT GCGTCGCCGA 
CTGCAAGATG ATGAATACAC GGCGGATAGC TACCAATCGT GGCTACAATC CCGTCCAACC 
TCCAACCTTG AAAAGCTGCA TTTTATCATT GGCCATGGTA TCCTGCGTGC CGAGCTGCGT 
GACGAAATAT ACTGCCAGAT ATGCAAGCAG CTGACGAACA ATCCATTGAA ATCATCACAT 
GCCAGAGGTT GGATTCTGTT ATCCCTGTGC GTCGGATGTT TTGCTCCGTC GGAAAAGTTT 
GTTAACTACT TGAGGGCCTT TATCCGTGAG GGTCCACCTG GATATGCACC GTACTGCGAG 
GAGCGACTGA AGCGTACTTT CAACAATGGA ACTCGCAATC AACCGCCTTC GTGGCTGGAG 
TTGCAGGCCA CCAAATCCAA AAAGCCCATC ATGCTGCCCA TTACCTTTAT GGATGGAAAT 
ACAAAGACTC TGTTGGCTGA TTCCGCCACC ACGGCCCGAG AACTATGCAA TCAACTGTCC 
GACAAGATAA GTCTCAAAGA TCAGTTTGGT TTTTCCCTGT ACATAGCTCT GTTCGACAAA 
GTTAGTTCTT TGGGAAGTGG CGGGGACCAC GTGATGGACG CCATCTCACA ATGTGAGCAG 
TATGCAAAGG AGCAGGGCGC CCAGGAGCGG AATGCCCCGT GGCGTCTGTT TTTCCGCAAG 
GAAATCTTTG CACCTTGGCA CGAACCCACA CACGATCAGG TGGCCACCAA TCTTATTTAC 
CAGCAGGTGG TGAGAGGCGT TAAGTTTGGC GAATATCGCT GCGACAAGGA GGAAGACTTG 
GCCATGATAG CTGCGCAGCA ATATTTCATC GAATACTCAA CCGATATGTC AATGGAGCGG 
CTGTTTACCC TTCTGCCCAA CTTTATACCC GACTTTTGTC TCAGCGGAGT GGACAAGGCC 
ATTGAACGTT GGGCTGCTTT GGTTCTGCAG GCCTATAAAA AATCGTACTA TGTCAAGGAC 
AAGATCGCAC CCTTGAAAAT TAAGGAGGAT ATAGTTAGCT ACGCCAAGTA CAAGTGGCCT 
CTGCTCTTTT CTCGTTTCTA CGAAGCGTAT CGAAACTCCG GTCCGAATCT GCCCAAAAAC 
GATGTCATCA TTGCGGTGAA CTGGACCGGC GTGTATGTGG TTGATGATCA GGAGCAGGTG 
CTGTTGGAAC TGAGCTTCCC CGAGATTACT GCCGTGTCCA GTCAGAAGAC CAACAAGGTC 
TTCACCCAGA CTTTCAGCCT TTCCACTGTT CGAGGCGAGG AGTTCACCTT CCAGAGCCCC 
AATGCGGAGG ATATCCGTGA TCTGGTTGTC TACTTCTTAG ATGGCCTGAA GAAACGATCG 
AAATATGTCA TAGCCTTGCA GGACTATCGT GCGCCTTCGG ATGGAACAAG TTTTCTGTCT 
TTCTTTAAGG GAGATCTGAT CATACTAGAG GACGAATCCT GTGGAGAATC AGTACTGAAC 
AACGGCTGGT GCATAGGACG CTGCGATCGT TCCCAGGAGC GAGGAGACTT CCCCGCCGAA 
ACCGTGTACG TGCTGCCCAC CCTTAGCAAA CCGCCGCAGG ATATTTTGGC CCTGTTCAAC 
ATCGAGGAGG CGCATCATGG ACGACGTCTA AGCATGGCTT CCAATGGTGG AGCTGTGGAA 
CCAAGGGATC GACCTCATAC GCTGATGGAG TATGCCCTGG ATCACTTCCG TTTGCCACCG 
AAACGAACAA TGTCGAAAAC ACTCACTCTG AGTTCAAAAC GGAGTGAGGA ATTGTGGCGC 
TACTCCCGGG ATCCCATTAA GGCACCGCTG CTGCGCAAGT TGCAAAGCAA GGAAGAGTTT 
GCCGAGGAGG CCTGCTTCGC GTTTGCCGCC ATACTGAAAT ATATGGGCGA TCTGCCCTCC 
AAGCGTCCGC GGATGGGCAA CGAAATAACC GATCACATAT TTGATGGACC TCTGAAGCAC 
GAAATTCTGC GCGACGAGAT CTACTGCCAA CTGATGAAGC AGCTAACGGA CAATCGCAAC 
CGAATGTCCG AGGAGAGGGG CTGGGAGCTG ATGTGGTTGG CCACGGGACT GTTTGCCTGT 
TCACAGGGTT TGCTCAAGGA ATTGCTATTA TTCCTGCGCA CCAGGCGTCA TCCCATTTCA 
CAGGACTCAA TGCATCGACT ACAGAAAACT ATACGCCATG GACAACGCAA ATATCCGCCG 
CATCAGGTGG AGGTGGAAGC CATACAGCAT AAAACCACAC AGATCTTCCA CAAGGTCTAC 
TTCCCGGACG ACACAGACGA GGCCTTCGAA GTGGACAGCT CCACTCGAGC GAAGGATTTC 
TGCAACAACA TCTCGCAGCG CCTTTCGCTG CGCACCAGCG AGGGCTTCTC CCTGTTTGTA 
AAGATCGCGG ATAAGGTCAT CTCTGTTCCC GAAGGTGATT TCTTCTTCGA TTTCGTACGC 
CATTTGACCG ACTGGATTAA AAAAGCGCGT CCCATACGAG ATGGAGCGAA TCCGCAGTTC 
ACTTACCAGG TCTTCTTCAT GAAGAAACTG TGGACGAACA CAGTGCCAGG CAAGGATCGG 
AATGCCGATC TTATATTCCA CTATCATCAG GAGTTGCCCA AGCTGTTGCG TGGCTATCAC 
AAGTGTTCCA GGGAGGAGGC GGCTAAGCTG GCCGCTCTAG TCTTCCGTGT GCGCTTTGGT 
GAAAATAAAC AAGAACTGCA GGCCATACCA CAAATGCTGA GGGAACTTAT TCCATCGGAT 
ATTATGAAAA TACAGAGCAC TAGCGAATGG AAACGATCGA TTGTTGCATC TTATAACCAG 
GATGGGGGCA TGACTTCCGA AGACGCGAAG GTGGCCTTCT TGAAGATTGT ATATAGATGG 
CCAACTTTTG GCTCCGCTTT CTTTGAGGTG AAGCAAACCA CCGAACCGAA TTATCCCGAA 
ATGCTATTGA TAGCCATTAA CAAACACGGC GTGAGTCTCA TACATCCAGT GACCAAGGAC 
ATTTTGGTTA CGCATCCATT TACACGCATC TCGAATTGGT CATCGGGCAA TACATATTTC 
CACATGACCA TCGGAAATCT GGTTAGGGGC TCGAAATTGT TATGTGAAAC GTCGCTGGGC 
TACAAGATGG ACGATCTGCT GACTTCATAT ATCTCGTTGA TGTTGACCAA TATGAACAAA 
AATCGAACCA TTCGAGCCAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ck ( NM_001299021.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285950.1 Drosophila melanogaster crinkled (ck), transcript variant D, mRNA.

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CloneID	OFa15890
Clone ID Related Accession (Same CDS sequence)	NM_001299020.1 , NM_165099.3 , NM_001299021.1 , NM_078847.4
Accession Version	NM_001299021.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	crinkled, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGATCG TAACACGTGG TGACTACATC TGGATAGAGC CCGCATCTGG GCGAGAATTC 
GATGTGGCCA TTGGAGCACG TGTCGTCTCC GCCGAGGGTC GTCGCATCCA GGTGCGCGAT 
GACGATGGCG ACGAGGTGTG GCTGGCACCC GAGCGCCGCA TCAAGGCCAT GCACGCGTCC 
TCCGTTCAGG GTGTGGAGGA CATGATATCC CTGGGTGATC TGCACGAGGC TGGCATCTTG 
CGGAATCTGC TCATCCGCTA CAAGGAGAAT CTGATATACA CTTACACGGG CTCCATACTT 
GTTGCCGTTA ATCCCTACCA GATTCTGCCC ATCTACACAG GTGATCAGAT CAAGCTCTAT 
AAGGAGCGGA AAATCGGTGA GCTACCGCCG CATATCTTTG CAATTGGCGA CAATGCGTAT 
GCGCATATGA AACGATATCG CCAGGATCAG TGTATCGTTA TTTCCGGAGA GTCTGGAGCT 
GGAAAGACGG AGAGCACAAA GTTGATCCTG CAGTATCTGG CTGCGATTAG TGGCAAACAC 
TCATGGATCG AACAGCAAAT CCTTGAAGCG AATCCCATTT TGGAAGCTTT CGGAAATGCC 
AAAACCATTC GAAATGATAA TTCATCGCGT TTTGGCAAAT ACATAGATAT ACACTTTAGC 
GCCAATGGCG TGATCGAGGG AGCCAAAATA GAACAGTACC TGCTGGAGAA GTCGCGAATT 
GTTTCCCAAA ATCATAGCGA ACGAAATTAT CATGTCTTTT ACTGCATTTT GGCCGGACTG 
TCGGCAGATG AGAAGAGTCG TCTCGATCTT GGCATGGCTG CTGATTACAA ATATCTGACT 
GGTGGTAATA GCATAACCTG CGAGGGACGC GATGACGCCG CCGAATTCTC TGACATTCGA 
TCTGCCATGA AGGTTTTGCT CTTCTCCGAT CAGGAAATCT GGGAGATAAT TAAACTCCTT 
GCTGCCCTTC TCCACTGCGG CAACATCAAA TATAAGGCGA CTGTGGTGGA TAATCTGGAT 
GCTACCGAAA TACCGGAACA CATAAATGTG GAGCGTGTGG CCGGGTTACT TGGACTTCCC 
ATTCAGCCGC TAATTGATGC TCTAACACGT CGAACACTTT TTGCTCATGG AGAAACCGTG 
GTGTCAACTC TGTCGCGCGA TCAATCCGTG GATGTGCGCG ATGCCTTTGT GAAAGGCATA 
TACGGACGTA TGTTTGTGCA CATCGTTCGA AAGATCAATA CGGCAATTTT CAAGCCGCGC 
GGCACATCCC GAAATGCCAT CGGAGTTCTC GACATCTTTG GTTTTGAGAA CTTCGATCAG 
AATAGCTTTG AGCAGTTTTG CATCAACTAT GCCAATGAGA ATCTGCAGCA GTTCTTCGTG 
CAGCATATAT TTAAACTTGA GCAGGAAGAG TATAACCACG AGGCCATCAA CTGGCAACAC 
ATTGAGTTCG TAGACAATCA GGATGCGCTC GACTTGATAG CTATTAAGCA GCTCAATATT 
ATGGCTTTAA TCGATGAGGA GGCCCGGTTT CCCAAGGGCA CGGATCAGAC GATGCTGGCC 
AAATTGCACA AAACCCATGG ATCGCACAAG AACTATTTGA AGCCCAAGTC GGATATCAAC 
ACCAGCTTTG GACTGAATCA CTTTGCCGGT GTGGTGTTCT ACGACACACG AGGATTTCTA 
GATAAAAACC GGGACACCTT CAGTCCCGAT CTATTGCATT TGGTTAGCCA AAGTACGAAC 
AAGTTCCTCC GACAAATCTT TGCTCAGGAC ATAGAGATGG GTGCAGAGAC GCGAAAGCGA 
ACACCCACAC TCTCCACACA GTTTCGAAAA TCTCTCGATG CGCTTATGAA GACGCTCAGC 
AGTTGCCAGC CATTCTTTAT TCGTTGTATC AAACCCAACG AGCTGAAGAA GCCAATGATG 
TTTGACCGCG GCTTGTGTTG CCGTCAGCTG CGATATTCCG GCATGATGGA AACGATCCGA 
ATCCGTCGCG CTGGATATCC CATTCGACAT GGATTCAGAG AGTTCGTCGA GCGTTATCGC 
TTCCTGATAC CGGGAGTTCC ACCAGCTCAT CGCACGGATT GCCAGGCGGC GACTTCGAGG 
ATTTGTGCCG TGGTTTTGGG CAAGTCCGAC TATCAGCTGG GCCACACCAA GGTCTTTCTC 
AAGGACGCCC ACGATCTGTT TCTGGAGCAG GAGAGGGATC GCGTGCTGAC GCGAAAAATC 
CTCATTCTGC AGCGCAGCAT TCGTGGTTGG GTGTACCGAC GAAGGTTTCT ACGCCTGAGA 
GCTGCTGCTA TCACTGTGCA GCGATTCTGG AAGGGCTATG CTCAACGTAA ACGATACAGG 
AACATGCGAG TGGGCTACAT GCGTTTGCAA GCGTTGATCC GTTCGAGAGT TCTGTCACAT 
CGTTTCCGCC ATCTGAGGGG CCACATTGTC GGGTTGCAAG CCCATGCCCG AGGATATTTG 
GTGAGGAGAG AGTATGGTCA CAAGATGTGG GCCGTCATTA AGATCCAGTC TCATGTGCGG 
CGTATGATTG CCATGCGGCG CTACCGAAAA CTTCGATTGG AGCACAAACA GTTTGCCGAA 
GTTCTTCAGC TGCGCAAACT GGAGGAGCAG GAACTGCTGC ATCGTGGCAA TAAGCATGCC 
CGAGAAATTG CCGAGCAGCA TTACCGAGAT CGATTGCACG AGCTGGAGCG TCGGGAAATT 
CAGGAGCAAT TGGAGAATCG TCGTCGAGTG GAGGTCAATA TGAATATTAT CAATGATGCA 
GCTCGCAAAC AGGAGGAGCC GGTGGACGAT GGCAAACTGG TGGAGGCCAT GTTTGACTTC 
CTTCCCGATT CCAGTAGCGA TGCCCCTACT CCGCATGGTG GACGTGAAAC GTCCGTGTTC 
AACGATCTGC CTCACGCGCA AAACGTCAAC CAGGACGATA TCATTGCACC CATACACATA 
TCCGAGGATG AGGAGGATCT ATCGGAATTC AAGTTCCAGA AATTCGCTGC CACCTACTTC 
CAAGGAAATG TCAATCATCA GTATGCTAAG AAAGCTTTGA AGCATCCCCT ACTTCCGCTC 
CATACGCAAG GCGATCAACT TGCTGCACAG GCATTGTGGA TAACCATACT GAGGTTTACA 
GGTGATATGC CGGAGCCGAA GTATCACACA ATGGATCGTA TGGACACCAC TTCGGTCATG 
TCCAAGGTCA CTGCCACCTT GGGGCGCAAT TTCATCAGGA GTAAGGAGTT CCAAGAGGCT 
CAGCTTATGG GCCTGGATCC AGATGCATTC CTTAAGCAAA AGCCACGATC GATCCGCCAC 
AAACTCGTAT CCCTGACGTT GAAGCGAAAG AACAAATTGG GCGAGGATGT GCGTCGCCGA 
CTGCAAGATG ATGAATACAC GGCGGATAGC TACCAATCGT GGCTACAATC CCGTCCAACC 
TCCAACCTTG AAAAGCTGCA TTTTATCATT GGCCATGGTA TCCTGCGTGC CGAGCTGCGT 
GACGAAATAT ACTGCCAGAT ATGCAAGCAG CTGACGAACA ATCCATTGAA ATCATCACAT 
GCCAGAGGTT GGATTCTGTT ATCCCTGTGC GTCGGATGTT TTGCTCCGTC GGAAAAGTTT 
GTTAACTACT TGAGGGCCTT TATCCGTGAG GGTCCACCTG GATATGCACC GTACTGCGAG 
GAGCGACTGA AGCGTACTTT CAACAATGGA ACTCGCAATC AACCGCCTTC GTGGCTGGAG 
TTGCAGGCCA CCAAATCCAA AAAGCCCATC ATGCTGCCCA TTACCTTTAT GGATGGAAAT 
ACAAAGACTC TGTTGGCTGA TTCCGCCACC ACGGCCCGAG AACTATGCAA TCAACTGTCC 
GACAAGATAA GTCTCAAAGA TCAGTTTGGT TTTTCCCTGT ACATAGCTCT GTTCGACAAA 
GTTAGTTCTT TGGGAAGTGG CGGGGACCAC GTGATGGACG CCATCTCACA ATGTGAGCAG 
TATGCAAAGG AGCAGGGCGC CCAGGAGCGG AATGCCCCGT GGCGTCTGTT TTTCCGCAAG 
GAAATCTTTG CACCTTGGCA CGAACCCACA CACGATCAGG TGGCCACCAA TCTTATTTAC 
CAGCAGGTGG TGAGAGGCGT TAAGTTTGGC GAATATCGCT GCGACAAGGA GGAAGACTTG 
GCCATGATAG CTGCGCAGCA ATATTTCATC GAATACTCAA CCGATATGTC AATGGAGCGG 
CTGTTTACCC TTCTGCCCAA CTTTATACCC GACTTTTGTC TCAGCGGAGT GGACAAGGCC 
ATTGAACGTT GGGCTGCTTT GGTTCTGCAG GCCTATAAAA AATCGTACTA TGTCAAGGAC 
AAGATCGCAC CCTTGAAAAT TAAGGAGGAT ATAGTTAGCT ACGCCAAGTA CAAGTGGCCT 
CTGCTCTTTT CTCGTTTCTA CGAAGCGTAT CGAAACTCCG GTCCGAATCT GCCCAAAAAC 
GATGTCATCA TTGCGGTGAA CTGGACCGGC GTGTATGTGG TTGATGATCA GGAGCAGGTG 
CTGTTGGAAC TGAGCTTCCC CGAGATTACT GCCGTGTCCA GTCAGAAGAC CAACAAGGTC 
TTCACCCAGA CTTTCAGCCT TTCCACTGTT CGAGGCGAGG AGTTCACCTT CCAGAGCCCC 
AATGCGGAGG ATATCCGTGA TCTGGTTGTC TACTTCTTAG ATGGCCTGAA GAAACGATCG 
AAATATGTCA TAGCCTTGCA GGACTATCGT GCGCCTTCGG ATGGAACAAG TTTTCTGTCT 
TTCTTTAAGG GAGATCTGAT CATACTAGAG GACGAATCCT GTGGAGAATC AGTACTGAAC 
AACGGCTGGT GCATAGGACG CTGCGATCGT TCCCAGGAGC GAGGAGACTT CCCCGCCGAA 
ACCGTGTACG TGCTGCCCAC CCTTAGCAAA CCGCCGCAGG ATATTTTGGC CCTGTTCAAC 
ATCGAGGAGG CGCATCATGG ACGACGTCTA AGCATGGCTT CCAATGGTGG AGCTGTGGAA 
CCAAGGGATC GACCTCATAC GCTGATGGAG TATGCCCTGG ATCACTTCCG TTTGCCACCG 
AAACGAACAA TGTCGAAAAC ACTCACTCTG AGTTCAAAAC GGAGTGAGGA ATTGTGGCGC 
TACTCCCGGG ATCCCATTAA GGCACCGCTG CTGCGCAAGT TGCAAAGCAA GGAAGAGTTT 
GCCGAGGAGG CCTGCTTCGC GTTTGCCGCC ATACTGAAAT ATATGGGCGA TCTGCCCTCC 
AAGCGTCCGC GGATGGGCAA CGAAATAACC GATCACATAT TTGATGGACC TCTGAAGCAC 
GAAATTCTGC GCGACGAGAT CTACTGCCAA CTGATGAAGC AGCTAACGGA CAATCGCAAC 
CGAATGTCCG AGGAGAGGGG CTGGGAGCTG ATGTGGTTGG CCACGGGACT GTTTGCCTGT 
TCACAGGGTT TGCTCAAGGA ATTGCTATTA TTCCTGCGCA CCAGGCGTCA TCCCATTTCA 
CAGGACTCAA TGCATCGACT ACAGAAAACT ATACGCCATG GACAACGCAA ATATCCGCCG 
CATCAGGTGG AGGTGGAAGC CATACAGCAT AAAACCACAC AGATCTTCCA CAAGGTCTAC 
TTCCCGGACG ACACAGACGA GGCCTTCGAA GTGGACAGCT CCACTCGAGC GAAGGATTTC 
TGCAACAACA TCTCGCAGCG CCTTTCGCTG CGCACCAGCG AGGGCTTCTC CCTGTTTGTA 
AAGATCGCGG ATAAGGTCAT CTCTGTTCCC GAAGGTGATT TCTTCTTCGA TTTCGTACGC 
CATTTGACCG ACTGGATTAA AAAAGCGCGT CCCATACGAG ATGGAGCGAA TCCGCAGTTC 
ACTTACCAGG TCTTCTTCAT GAAGAAACTG TGGACGAACA CAGTGCCAGG CAAGGATCGG 
AATGCCGATC TTATATTCCA CTATCATCAG GAGTTGCCCA AGCTGTTGCG TGGCTATCAC 
AAGTGTTCCA GGGAGGAGGC GGCTAAGCTG GCCGCTCTAG TCTTCCGTGT GCGCTTTGGT 
GAAAATAAAC AAGAACTGCA GGCCATACCA CAAATGCTGA GGGAACTTAT TCCATCGGAT 
ATTATGAAAA TACAGAGCAC TAGCGAATGG AAACGATCGA TTGTTGCATC TTATAACCAG 
GATGGGGGCA TGACTTCCGA AGACGCGAAG GTGGCCTTCT TGAAGATTGT ATATAGATGG 
CCAACTTTTG GCTCCGCTTT CTTTGAGGTG AAGCAAACCA CCGAACCGAA TTATCCCGAA 
ATGCTATTGA TAGCCATTAA CAAACACGGC GTGAGTCTCA TACATCCAGT GACCAAGGAC 
ATTTTGGTTA CGCATCCATT TACACGCATC TCGAATTGGT CATCGGGCAA TACATATTTC 
CACATGACCA TCGGAAATCT GGTTAGGGGC TCGAAATTGT TATGTGAAAC GTCGCTGGGC 
TACAAGATGG ACGATCTGCT GACTTCATAT ATCTCGTTGA TGTTGACCAA TATGAACAAA 
AATCGAACCA TTCGAGCCAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285950.1
CDS	450..6953
Translation	MVIVTRGDYIWIEPASGREFDVAIGARVVSAEGRRIQVRDDDGDEVWLAPERRIKAMHASSVQGVEDMISLGDLHEAGILRNLLIRYKENLIYTYTGSILVAVNPYQILPIYTGDQIKLYKERKIGELPPHIFAIGDNAYAHMKRYRQDQCIVISGESGAGKTESTKLILQYLAAISGKHSWIEQQILEANPILEAFGNAKTIRNDNSSRFGKYIDIHFSANGVIEGAKIEQYLLEKSRIVSQNHSERNYHVFYCILAGLSADEKSRLDLGMAADYKYLTGGNSITCEGRDDAAEFSDIRSAMKVLLFSDQEIWEIIKLLAALLHCGNIKYKATVVDNLDATEIPEHINVERVAGLLGLPIQPLIDALTRRTLFAHGETVVSTLSRDQSVDVRDAFVKGIYGRMFVHIVRKINTAIFKPRGTSRNAIGVLDIFGFENFDQNSFEQFCINYANENLQQFFVQHIFKLEQEEYNHEAINWQHIEFVDNQDALDLIAIKQLNIMALIDEEARFPKGTDQTMLAKLHKTHGSHKNYLKPKSDINTSFGLNHFAGVVFYDTRGFLDKNRDTFSPDLLHLVSQSTNKFLRQIFAQDIEMGAETRKRTPTLSTQFRKSLDALMKTLSSCQPFFIRCIKPNELKKPMMFDRGLCCRQLRYSGMMETIRIRRAGYPIRHGFREFVERYRFLIPGVPPAHRTDCQAATSRICAVVLGKSDYQLGHTKVFLKDAHDLFLEQERDRVLTRKILILQRSIRGWVYRRRFLRLRAAAITVQRFWKGYAQRKRYRNMRVGYMRLQALIRSRVLSHRFRHLRGHIVGLQAHARGYLVRREYGHKMWAVIKIQSHVRRMIAMRRYRKLRLEHKQFAEVLQLRKLEEQELLHRGNKHAREIAEQHYRDRLHELERREIQEQLENRRRVEVNMNIINDAARKQEEPVDDGKLVEAMFDFLPDSSSDAPTPHGGRETSVFNDLPHAQNVNQDDIIAPIHISEDEEDLSEFKFQKFAATYFQGNVNHQYAKKALKHPLLPLHTQGDQLAAQALWITILRFTGDMPEPKYHTMDRMDTTSVMSKVTATLGRNFIRSKEFQEAQLMGLDPDAFLKQKPRSIRHKLVSLTLKRKNKLGEDVRRRLQDDEYTADSYQSWLQSRPTSNLEKLHFIIGHGILRAELRDEIYCQICKQLTNNPLKSSHARGWILLSLCVGCFAPSEKFVNYLRAFIREGPPGYAPYCEERLKRTFNNGTRNQPPSWLELQATKSKKPIMLPITFMDGNTKTLLADSATTARELCNQLSDKISLKDQFGFSLYIALFDKVSSLGSGGDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEIFAPWHEPTHDQVATNLIYQQVVRGVKFGEYRCDKEEDLAMIAAQQYFIEYSTDMSMERLFTLLPNFIPDFCLSGVDKAIERWAALVLQAYKKSYYVKDKIAPLKIKEDIVSYAKYKWPLLFSRFYEAYRNSGPNLPKNDVIIAVNWTGVYVVDDQEQVLLELSFPEITAVSSQKTNKVFTQTFSLSTVRGEEFTFQSPNAEDIRDLVVYFLDGLKKRSKYVIALQDYRAPSDGTSFLSFFKGDLIILEDESCGESVLNNGWCIGRCDRSQERGDFPAETVYVLPTLSKPPQDILALFNIEEAHHGRRLSMASNGGAVEPRDRPHTLMEYALDHFRLPPKRTMSKTLTLSSKRSEELWRYSRDPIKAPLLRKLQSKEEFAEEACFAFAAILKYMGDLPSKRPRMGNEITDHIFDGPLKHEILRDEIYCQLMKQLTDNRNRMSEERGWELMWLATGLFACSQGLLKELLLFLRTRRHPISQDSMHRLQKTIRHGQRKYPPHQVEVEAIQHKTTQIFHKVYFPDDTDEAFEVDSSTRAKDFCNNISQRLSLRTSEGFSLFVKIADKVISVPEGDFFFDFVRHLTDWIKKARPIRDGANPQFTYQVFFMKKLWTNTVPGKDRNADLIFHYHQELPKLLRGYHKCSREEAAKLAALVFRVRFGENKQELQAIPQMLRELIPSDIMKIQSTSEWKRSIVASYNQDGGMTSEDAKVAFLKIVYRWPTFGSAFFEVKQTTEPNYPEMLLIAINKHGVSLIHPVTKDILVTHPFTRISNWSSGNTYFHMTIGNLVRGSKLLCETSLGYKMDDLLTSYISLMLTNMNKNRTIRAN

Target ORF information:

RefSeq Version	NM_001299021.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster crinkled (ck), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299021.1

ORF Insert Sequence:

ATGGTGATCG TAACACGTGG TGACTACATC TGGATAGAGC CCGCATCTGG GCGAGAATTC 
GATGTGGCCA TTGGAGCACG TGTCGTCTCC GCCGAGGGTC GTCGCATCCA GGTGCGCGAT 
GACGATGGCG ACGAGGTGTG GCTGGCACCC GAGCGCCGCA TCAAGGCCAT GCACGCGTCC 
TCCGTTCAGG GTGTGGAGGA CATGATATCC CTGGGTGATC TGCACGAGGC TGGCATCTTG 
CGGAATCTGC TCATCCGCTA CAAGGAGAAT CTGATATACA CTTACACGGG CTCCATACTT 
GTTGCCGTTA ATCCCTACCA GATTCTGCCC ATCTACACAG GTGATCAGAT CAAGCTCTAT 
AAGGAGCGGA AAATCGGTGA GCTACCGCCG CATATCTTTG CAATTGGCGA CAATGCGTAT 
GCGCATATGA AACGATATCG CCAGGATCAG TGTATCGTTA TTTCCGGAGA GTCTGGAGCT 
GGAAAGACGG AGAGCACAAA GTTGATCCTG CAGTATCTGG CTGCGATTAG TGGCAAACAC 
TCATGGATCG AACAGCAAAT CCTTGAAGCG AATCCCATTT TGGAAGCTTT CGGAAATGCC 
AAAACCATTC GAAATGATAA TTCATCGCGT TTTGGCAAAT ACATAGATAT ACACTTTAGC 
GCCAATGGCG TGATCGAGGG AGCCAAAATA GAACAGTACC TGCTGGAGAA GTCGCGAATT 
GTTTCCCAAA ATCATAGCGA ACGAAATTAT CATGTCTTTT ACTGCATTTT GGCCGGACTG 
TCGGCAGATG AGAAGAGTCG TCTCGATCTT GGCATGGCTG CTGATTACAA ATATCTGACT 
GGTGGTAATA GCATAACCTG CGAGGGACGC GATGACGCCG CCGAATTCTC TGACATTCGA 
TCTGCCATGA AGGTTTTGCT CTTCTCCGAT CAGGAAATCT GGGAGATAAT TAAACTCCTT 
GCTGCCCTTC TCCACTGCGG CAACATCAAA TATAAGGCGA CTGTGGTGGA TAATCTGGAT 
GCTACCGAAA TACCGGAACA CATAAATGTG GAGCGTGTGG CCGGGTTACT TGGACTTCCC 
ATTCAGCCGC TAATTGATGC TCTAACACGT CGAACACTTT TTGCTCATGG AGAAACCGTG 
GTGTCAACTC TGTCGCGCGA TCAATCCGTG GATGTGCGCG ATGCCTTTGT GAAAGGCATA 
TACGGACGTA TGTTTGTGCA CATCGTTCGA AAGATCAATA CGGCAATTTT CAAGCCGCGC 
GGCACATCCC GAAATGCCAT CGGAGTTCTC GACATCTTTG GTTTTGAGAA CTTCGATCAG 
AATAGCTTTG AGCAGTTTTG CATCAACTAT GCCAATGAGA ATCTGCAGCA GTTCTTCGTG 
CAGCATATAT TTAAACTTGA GCAGGAAGAG TATAACCACG AGGCCATCAA CTGGCAACAC 
ATTGAGTTCG TAGACAATCA GGATGCGCTC GACTTGATAG CTATTAAGCA GCTCAATATT 
ATGGCTTTAA TCGATGAGGA GGCCCGGTTT CCCAAGGGCA CGGATCAGAC GATGCTGGCC 
AAATTGCACA AAACCCATGG ATCGCACAAG AACTATTTGA AGCCCAAGTC GGATATCAAC 
ACCAGCTTTG GACTGAATCA CTTTGCCGGT GTGGTGTTCT ACGACACACG AGGATTTCTA 
GATAAAAACC GGGACACCTT CAGTCCCGAT CTATTGCATT TGGTTAGCCA AAGTACGAAC 
AAGTTCCTCC GACAAATCTT TGCTCAGGAC ATAGAGATGG GTGCAGAGAC GCGAAAGCGA 
ACACCCACAC TCTCCACACA GTTTCGAAAA TCTCTCGATG CGCTTATGAA GACGCTCAGC 
AGTTGCCAGC CATTCTTTAT TCGTTGTATC AAACCCAACG AGCTGAAGAA GCCAATGATG 
TTTGACCGCG GCTTGTGTTG CCGTCAGCTG CGATATTCCG GCATGATGGA AACGATCCGA 
ATCCGTCGCG CTGGATATCC CATTCGACAT GGATTCAGAG AGTTCGTCGA GCGTTATCGC 
TTCCTGATAC CGGGAGTTCC ACCAGCTCAT CGCACGGATT GCCAGGCGGC GACTTCGAGG 
ATTTGTGCCG TGGTTTTGGG CAAGTCCGAC TATCAGCTGG GCCACACCAA GGTCTTTCTC 
AAGGACGCCC ACGATCTGTT TCTGGAGCAG GAGAGGGATC GCGTGCTGAC GCGAAAAATC 
CTCATTCTGC AGCGCAGCAT TCGTGGTTGG GTGTACCGAC GAAGGTTTCT ACGCCTGAGA 
GCTGCTGCTA TCACTGTGCA GCGATTCTGG AAGGGCTATG CTCAACGTAA ACGATACAGG 
AACATGCGAG TGGGCTACAT GCGTTTGCAA GCGTTGATCC GTTCGAGAGT TCTGTCACAT 
CGTTTCCGCC ATCTGAGGGG CCACATTGTC GGGTTGCAAG CCCATGCCCG AGGATATTTG 
GTGAGGAGAG AGTATGGTCA CAAGATGTGG GCCGTCATTA AGATCCAGTC TCATGTGCGG 
CGTATGATTG CCATGCGGCG CTACCGAAAA CTTCGATTGG AGCACAAACA GTTTGCCGAA 
GTTCTTCAGC TGCGCAAACT GGAGGAGCAG GAACTGCTGC ATCGTGGCAA TAAGCATGCC 
CGAGAAATTG CCGAGCAGCA TTACCGAGAT CGATTGCACG AGCTGGAGCG TCGGGAAATT 
CAGGAGCAAT TGGAGAATCG TCGTCGAGTG GAGGTCAATA TGAATATTAT CAATGATGCA 
GCTCGCAAAC AGGAGGAGCC GGTGGACGAT GGCAAACTGG TGGAGGCCAT GTTTGACTTC 
CTTCCCGATT CCAGTAGCGA TGCCCCTACT CCGCATGGTG GACGTGAAAC GTCCGTGTTC 
AACGATCTGC CTCACGCGCA AAACGTCAAC CAGGACGATA TCATTGCACC CATACACATA 
TCCGAGGATG AGGAGGATCT ATCGGAATTC AAGTTCCAGA AATTCGCTGC CACCTACTTC 
CAAGGAAATG TCAATCATCA GTATGCTAAG AAAGCTTTGA AGCATCCCCT ACTTCCGCTC 
CATACGCAAG GCGATCAACT TGCTGCACAG GCATTGTGGA TAACCATACT GAGGTTTACA 
GGTGATATGC CGGAGCCGAA GTATCACACA ATGGATCGTA TGGACACCAC TTCGGTCATG 
TCCAAGGTCA CTGCCACCTT GGGGCGCAAT TTCATCAGGA GTAAGGAGTT CCAAGAGGCT 
CAGCTTATGG GCCTGGATCC AGATGCATTC CTTAAGCAAA AGCCACGATC GATCCGCCAC 
AAACTCGTAT CCCTGACGTT GAAGCGAAAG AACAAATTGG GCGAGGATGT GCGTCGCCGA 
CTGCAAGATG ATGAATACAC GGCGGATAGC TACCAATCGT GGCTACAATC CCGTCCAACC 
TCCAACCTTG AAAAGCTGCA TTTTATCATT GGCCATGGTA TCCTGCGTGC CGAGCTGCGT 
GACGAAATAT ACTGCCAGAT ATGCAAGCAG CTGACGAACA ATCCATTGAA ATCATCACAT 
GCCAGAGGTT GGATTCTGTT ATCCCTGTGC GTCGGATGTT TTGCTCCGTC GGAAAAGTTT 
GTTAACTACT TGAGGGCCTT TATCCGTGAG GGTCCACCTG GATATGCACC GTACTGCGAG 
GAGCGACTGA AGCGTACTTT CAACAATGGA ACTCGCAATC AACCGCCTTC GTGGCTGGAG 
TTGCAGGCCA CCAAATCCAA AAAGCCCATC ATGCTGCCCA TTACCTTTAT GGATGGAAAT 
ACAAAGACTC TGTTGGCTGA TTCCGCCACC ACGGCCCGAG AACTATGCAA TCAACTGTCC 
GACAAGATAA GTCTCAAAGA TCAGTTTGGT TTTTCCCTGT ACATAGCTCT GTTCGACAAA 
GTTAGTTCTT TGGGAAGTGG CGGGGACCAC GTGATGGACG CCATCTCACA ATGTGAGCAG 
TATGCAAAGG AGCAGGGCGC CCAGGAGCGG AATGCCCCGT GGCGTCTGTT TTTCCGCAAG 
GAAATCTTTG CACCTTGGCA CGAACCCACA CACGATCAGG TGGCCACCAA TCTTATTTAC 
CAGCAGGTGG TGAGAGGCGT TAAGTTTGGC GAATATCGCT GCGACAAGGA GGAAGACTTG 
GCCATGATAG CTGCGCAGCA ATATTTCATC GAATACTCAA CCGATATGTC AATGGAGCGG 
CTGTTTACCC TTCTGCCCAA CTTTATACCC GACTTTTGTC TCAGCGGAGT GGACAAGGCC 
ATTGAACGTT GGGCTGCTTT GGTTCTGCAG GCCTATAAAA AATCGTACTA TGTCAAGGAC 
AAGATCGCAC CCTTGAAAAT TAAGGAGGAT ATAGTTAGCT ACGCCAAGTA CAAGTGGCCT 
CTGCTCTTTT CTCGTTTCTA CGAAGCGTAT CGAAACTCCG GTCCGAATCT GCCCAAAAAC 
GATGTCATCA TTGCGGTGAA CTGGACCGGC GTGTATGTGG TTGATGATCA GGAGCAGGTG 
CTGTTGGAAC TGAGCTTCCC CGAGATTACT GCCGTGTCCA GTCAGAAGAC CAACAAGGTC 
TTCACCCAGA CTTTCAGCCT TTCCACTGTT CGAGGCGAGG AGTTCACCTT CCAGAGCCCC 
AATGCGGAGG ATATCCGTGA TCTGGTTGTC TACTTCTTAG ATGGCCTGAA GAAACGATCG 
AAATATGTCA TAGCCTTGCA GGACTATCGT GCGCCTTCGG ATGGAACAAG TTTTCTGTCT 
TTCTTTAAGG GAGATCTGAT CATACTAGAG GACGAATCCT GTGGAGAATC AGTACTGAAC 
AACGGCTGGT GCATAGGACG CTGCGATCGT TCCCAGGAGC GAGGAGACTT CCCCGCCGAA 
ACCGTGTACG TGCTGCCCAC CCTTAGCAAA CCGCCGCAGG ATATTTTGGC CCTGTTCAAC 
ATCGAGGAGG CGCATCATGG ACGACGTCTA AGCATGGCTT CCAATGGTGG AGCTGTGGAA 
CCAAGGGATC GACCTCATAC GCTGATGGAG TATGCCCTGG ATCACTTCCG TTTGCCACCG 
AAACGAACAA TGTCGAAAAC ACTCACTCTG AGTTCAAAAC GGAGTGAGGA ATTGTGGCGC 
TACTCCCGGG ATCCCATTAA GGCACCGCTG CTGCGCAAGT TGCAAAGCAA GGAAGAGTTT 
GCCGAGGAGG CCTGCTTCGC GTTTGCCGCC ATACTGAAAT ATATGGGCGA TCTGCCCTCC 
AAGCGTCCGC GGATGGGCAA CGAAATAACC GATCACATAT TTGATGGACC TCTGAAGCAC 
GAAATTCTGC GCGACGAGAT CTACTGCCAA CTGATGAAGC AGCTAACGGA CAATCGCAAC 
CGAATGTCCG AGGAGAGGGG CTGGGAGCTG ATGTGGTTGG CCACGGGACT GTTTGCCTGT 
TCACAGGGTT TGCTCAAGGA ATTGCTATTA TTCCTGCGCA CCAGGCGTCA TCCCATTTCA 
CAGGACTCAA TGCATCGACT ACAGAAAACT ATACGCCATG GACAACGCAA ATATCCGCCG 
CATCAGGTGG AGGTGGAAGC CATACAGCAT AAAACCACAC AGATCTTCCA CAAGGTCTAC 
TTCCCGGACG ACACAGACGA GGCCTTCGAA GTGGACAGCT CCACTCGAGC GAAGGATTTC 
TGCAACAACA TCTCGCAGCG CCTTTCGCTG CGCACCAGCG AGGGCTTCTC CCTGTTTGTA 
AAGATCGCGG ATAAGGTCAT CTCTGTTCCC GAAGGTGATT TCTTCTTCGA TTTCGTACGC 
CATTTGACCG ACTGGATTAA AAAAGCGCGT CCCATACGAG ATGGAGCGAA TCCGCAGTTC 
ACTTACCAGG TCTTCTTCAT GAAGAAACTG TGGACGAACA CAGTGCCAGG CAAGGATCGG 
AATGCCGATC TTATATTCCA CTATCATCAG GAGTTGCCCA AGCTGTTGCG TGGCTATCAC 
AAGTGTTCCA GGGAGGAGGC GGCTAAGCTG GCCGCTCTAG TCTTCCGTGT GCGCTTTGGT 
GAAAATAAAC AAGAACTGCA GGCCATACCA CAAATGCTGA GGGAACTTAT TCCATCGGAT 
ATTATGAAAA TACAGAGCAC TAGCGAATGG AAACGATCGA TTGTTGCATC TTATAACCAG 
GATGGGGGCA TGACTTCCGA AGACGCGAAG GTGGCCTTCT TGAAGATTGT ATATAGATGG 
CCAACTTTTG GCTCCGCTTT CTTTGAGGTG AAGCAAACCA CCGAACCGAA TTATCCCGAA 
ATGCTATTGA TAGCCATTAA CAAACACGGC GTGAGTCTCA TACATCCAGT GACCAAGGAC 
ATTTTGGTTA CGCATCCATT TACACGCATC TCGAATTGGT CATCGGGCAA TACATATTTC 
CACATGACCA TCGGAAATCT GGTTAGGGGC TCGAAATTGT TATGTGAAAC GTCGCTGGGC 
TACAAGATGG ACGATCTGCT GACTTCATAT ATCTCGTTGA TGTTGACCAA TATGAACAAA 
AATCGAACCA TTCGAGCCAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ck ( NM_078847.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523571.1 Drosophila melanogaster crinkled (ck), transcript variant B, mRNA.

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CloneID	OFa15890
Clone ID Related Accession (Same CDS sequence)	NM_001299020.1 , NM_165099.3 , NM_001299021.1 , NM_078847.4
Accession Version	NM_078847.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	crinkled, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_078847.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGATCG TAACACGTGG TGACTACATC TGGATAGAGC CCGCATCTGG GCGAGAATTC 
GATGTGGCCA TTGGAGCACG TGTCGTCTCC GCCGAGGGTC GTCGCATCCA GGTGCGCGAT 
GACGATGGCG ACGAGGTGTG GCTGGCACCC GAGCGCCGCA TCAAGGCCAT GCACGCGTCC 
TCCGTTCAGG GTGTGGAGGA CATGATATCC CTGGGTGATC TGCACGAGGC TGGCATCTTG 
CGGAATCTGC TCATCCGCTA CAAGGAGAAT CTGATATACA CTTACACGGG CTCCATACTT 
GTTGCCGTTA ATCCCTACCA GATTCTGCCC ATCTACACAG GTGATCAGAT CAAGCTCTAT 
AAGGAGCGGA AAATCGGTGA GCTACCGCCG CATATCTTTG CAATTGGCGA CAATGCGTAT 
GCGCATATGA AACGATATCG CCAGGATCAG TGTATCGTTA TTTCCGGAGA GTCTGGAGCT 
GGAAAGACGG AGAGCACAAA GTTGATCCTG CAGTATCTGG CTGCGATTAG TGGCAAACAC 
TCATGGATCG AACAGCAAAT CCTTGAAGCG AATCCCATTT TGGAAGCTTT CGGAAATGCC 
AAAACCATTC GAAATGATAA TTCATCGCGT TTTGGCAAAT ACATAGATAT ACACTTTAGC 
GCCAATGGCG TGATCGAGGG AGCCAAAATA GAACAGTACC TGCTGGAGAA GTCGCGAATT 
GTTTCCCAAA ATCATAGCGA ACGAAATTAT CATGTCTTTT ACTGCATTTT GGCCGGACTG 
TCGGCAGATG AGAAGAGTCG TCTCGATCTT GGCATGGCTG CTGATTACAA ATATCTGACT 
GGTGGTAATA GCATAACCTG CGAGGGACGC GATGACGCCG CCGAATTCTC TGACATTCGA 
TCTGCCATGA AGGTTTTGCT CTTCTCCGAT CAGGAAATCT GGGAGATAAT TAAACTCCTT 
GCTGCCCTTC TCCACTGCGG CAACATCAAA TATAAGGCGA CTGTGGTGGA TAATCTGGAT 
GCTACCGAAA TACCGGAACA CATAAATGTG GAGCGTGTGG CCGGGTTACT TGGACTTCCC 
ATTCAGCCGC TAATTGATGC TCTAACACGT CGAACACTTT TTGCTCATGG AGAAACCGTG 
GTGTCAACTC TGTCGCGCGA TCAATCCGTG GATGTGCGCG ATGCCTTTGT GAAAGGCATA 
TACGGACGTA TGTTTGTGCA CATCGTTCGA AAGATCAATA CGGCAATTTT CAAGCCGCGC 
GGCACATCCC GAAATGCCAT CGGAGTTCTC GACATCTTTG GTTTTGAGAA CTTCGATCAG 
AATAGCTTTG AGCAGTTTTG CATCAACTAT GCCAATGAGA ATCTGCAGCA GTTCTTCGTG 
CAGCATATAT TTAAACTTGA GCAGGAAGAG TATAACCACG AGGCCATCAA CTGGCAACAC 
ATTGAGTTCG TAGACAATCA GGATGCGCTC GACTTGATAG CTATTAAGCA GCTCAATATT 
ATGGCTTTAA TCGATGAGGA GGCCCGGTTT CCCAAGGGCA CGGATCAGAC GATGCTGGCC 
AAATTGCACA AAACCCATGG ATCGCACAAG AACTATTTGA AGCCCAAGTC GGATATCAAC 
ACCAGCTTTG GACTGAATCA CTTTGCCGGT GTGGTGTTCT ACGACACACG AGGATTTCTA 
GATAAAAACC GGGACACCTT CAGTCCCGAT CTATTGCATT TGGTTAGCCA AAGTACGAAC 
AAGTTCCTCC GACAAATCTT TGCTCAGGAC ATAGAGATGG GTGCAGAGAC GCGAAAGCGA 
ACACCCACAC TCTCCACACA GTTTCGAAAA TCTCTCGATG CGCTTATGAA GACGCTCAGC 
AGTTGCCAGC CATTCTTTAT TCGTTGTATC AAACCCAACG AGCTGAAGAA GCCAATGATG 
TTTGACCGCG GCTTGTGTTG CCGTCAGCTG CGATATTCCG GCATGATGGA AACGATCCGA 
ATCCGTCGCG CTGGATATCC CATTCGACAT GGATTCAGAG AGTTCGTCGA GCGTTATCGC 
TTCCTGATAC CGGGAGTTCC ACCAGCTCAT CGCACGGATT GCCAGGCGGC GACTTCGAGG 
ATTTGTGCCG TGGTTTTGGG CAAGTCCGAC TATCAGCTGG GCCACACCAA GGTCTTTCTC 
AAGGACGCCC ACGATCTGTT TCTGGAGCAG GAGAGGGATC GCGTGCTGAC GCGAAAAATC 
CTCATTCTGC AGCGCAGCAT TCGTGGTTGG GTGTACCGAC GAAGGTTTCT ACGCCTGAGA 
GCTGCTGCTA TCACTGTGCA GCGATTCTGG AAGGGCTATG CTCAACGTAA ACGATACAGG 
AACATGCGAG TGGGCTACAT GCGTTTGCAA GCGTTGATCC GTTCGAGAGT TCTGTCACAT 
CGTTTCCGCC ATCTGAGGGG CCACATTGTC GGGTTGCAAG CCCATGCCCG AGGATATTTG 
GTGAGGAGAG AGTATGGTCA CAAGATGTGG GCCGTCATTA AGATCCAGTC TCATGTGCGG 
CGTATGATTG CCATGCGGCG CTACCGAAAA CTTCGATTGG AGCACAAACA GTTTGCCGAA 
GTTCTTCAGC TGCGCAAACT GGAGGAGCAG GAACTGCTGC ATCGTGGCAA TAAGCATGCC 
CGAGAAATTG CCGAGCAGCA TTACCGAGAT CGATTGCACG AGCTGGAGCG TCGGGAAATT 
CAGGAGCAAT TGGAGAATCG TCGTCGAGTG GAGGTCAATA TGAATATTAT CAATGATGCA 
GCTCGCAAAC AGGAGGAGCC GGTGGACGAT GGCAAACTGG TGGAGGCCAT GTTTGACTTC 
CTTCCCGATT CCAGTAGCGA TGCCCCTACT CCGCATGGTG GACGTGAAAC GTCCGTGTTC 
AACGATCTGC CTCACGCGCA AAACGTCAAC CAGGACGATA TCATTGCACC CATACACATA 
TCCGAGGATG AGGAGGATCT ATCGGAATTC AAGTTCCAGA AATTCGCTGC CACCTACTTC 
CAAGGAAATG TCAATCATCA GTATGCTAAG AAAGCTTTGA AGCATCCCCT ACTTCCGCTC 
CATACGCAAG GCGATCAACT TGCTGCACAG GCATTGTGGA TAACCATACT GAGGTTTACA 
GGTGATATGC CGGAGCCGAA GTATCACACA ATGGATCGTA TGGACACCAC TTCGGTCATG 
TCCAAGGTCA CTGCCACCTT GGGGCGCAAT TTCATCAGGA GTAAGGAGTT CCAAGAGGCT 
CAGCTTATGG GCCTGGATCC AGATGCATTC CTTAAGCAAA AGCCACGATC GATCCGCCAC 
AAACTCGTAT CCCTGACGTT GAAGCGAAAG AACAAATTGG GCGAGGATGT GCGTCGCCGA 
CTGCAAGATG ATGAATACAC GGCGGATAGC TACCAATCGT GGCTACAATC CCGTCCAACC 
TCCAACCTTG AAAAGCTGCA TTTTATCATT GGCCATGGTA TCCTGCGTGC CGAGCTGCGT 
GACGAAATAT ACTGCCAGAT ATGCAAGCAG CTGACGAACA ATCCATTGAA ATCATCACAT 
GCCAGAGGTT GGATTCTGTT ATCCCTGTGC GTCGGATGTT TTGCTCCGTC GGAAAAGTTT 
GTTAACTACT TGAGGGCCTT TATCCGTGAG GGTCCACCTG GATATGCACC GTACTGCGAG 
GAGCGACTGA AGCGTACTTT CAACAATGGA ACTCGCAATC AACCGCCTTC GTGGCTGGAG 
TTGCAGGCCA CCAAATCCAA AAAGCCCATC ATGCTGCCCA TTACCTTTAT GGATGGAAAT 
ACAAAGACTC TGTTGGCTGA TTCCGCCACC ACGGCCCGAG AACTATGCAA TCAACTGTCC 
GACAAGATAA GTCTCAAAGA TCAGTTTGGT TTTTCCCTGT ACATAGCTCT GTTCGACAAA 
GTTAGTTCTT TGGGAAGTGG CGGGGACCAC GTGATGGACG CCATCTCACA ATGTGAGCAG 
TATGCAAAGG AGCAGGGCGC CCAGGAGCGG AATGCCCCGT GGCGTCTGTT TTTCCGCAAG 
GAAATCTTTG CACCTTGGCA CGAACCCACA CACGATCAGG TGGCCACCAA TCTTATTTAC 
CAGCAGGTGG TGAGAGGCGT TAAGTTTGGC GAATATCGCT GCGACAAGGA GGAAGACTTG 
GCCATGATAG CTGCGCAGCA ATATTTCATC GAATACTCAA CCGATATGTC AATGGAGCGG 
CTGTTTACCC TTCTGCCCAA CTTTATACCC GACTTTTGTC TCAGCGGAGT GGACAAGGCC 
ATTGAACGTT GGGCTGCTTT GGTTCTGCAG GCCTATAAAA AATCGTACTA TGTCAAGGAC 
AAGATCGCAC CCTTGAAAAT TAAGGAGGAT ATAGTTAGCT ACGCCAAGTA CAAGTGGCCT 
CTGCTCTTTT CTCGTTTCTA CGAAGCGTAT CGAAACTCCG GTCCGAATCT GCCCAAAAAC 
GATGTCATCA TTGCGGTGAA CTGGACCGGC GTGTATGTGG TTGATGATCA GGAGCAGGTG 
CTGTTGGAAC TGAGCTTCCC CGAGATTACT GCCGTGTCCA GTCAGAAGAC CAACAAGGTC 
TTCACCCAGA CTTTCAGCCT TTCCACTGTT CGAGGCGAGG AGTTCACCTT CCAGAGCCCC 
AATGCGGAGG ATATCCGTGA TCTGGTTGTC TACTTCTTAG ATGGCCTGAA GAAACGATCG 
AAATATGTCA TAGCCTTGCA GGACTATCGT GCGCCTTCGG ATGGAACAAG TTTTCTGTCT 
TTCTTTAAGG GAGATCTGAT CATACTAGAG GACGAATCCT GTGGAGAATC AGTACTGAAC 
AACGGCTGGT GCATAGGACG CTGCGATCGT TCCCAGGAGC GAGGAGACTT CCCCGCCGAA 
ACCGTGTACG TGCTGCCCAC CCTTAGCAAA CCGCCGCAGG ATATTTTGGC CCTGTTCAAC 
ATCGAGGAGG CGCATCATGG ACGACGTCTA AGCATGGCTT CCAATGGTGG AGCTGTGGAA 
CCAAGGGATC GACCTCATAC GCTGATGGAG TATGCCCTGG ATCACTTCCG TTTGCCACCG 
AAACGAACAA TGTCGAAAAC ACTCACTCTG AGTTCAAAAC GGAGTGAGGA ATTGTGGCGC 
TACTCCCGGG ATCCCATTAA GGCACCGCTG CTGCGCAAGT TGCAAAGCAA GGAAGAGTTT 
GCCGAGGAGG CCTGCTTCGC GTTTGCCGCC ATACTGAAAT ATATGGGCGA TCTGCCCTCC 
AAGCGTCCGC GGATGGGCAA CGAAATAACC GATCACATAT TTGATGGACC TCTGAAGCAC 
GAAATTCTGC GCGACGAGAT CTACTGCCAA CTGATGAAGC AGCTAACGGA CAATCGCAAC 
CGAATGTCCG AGGAGAGGGG CTGGGAGCTG ATGTGGTTGG CCACGGGACT GTTTGCCTGT 
TCACAGGGTT TGCTCAAGGA ATTGCTATTA TTCCTGCGCA CCAGGCGTCA TCCCATTTCA 
CAGGACTCAA TGCATCGACT ACAGAAAACT ATACGCCATG GACAACGCAA ATATCCGCCG 
CATCAGGTGG AGGTGGAAGC CATACAGCAT AAAACCACAC AGATCTTCCA CAAGGTCTAC 
TTCCCGGACG ACACAGACGA GGCCTTCGAA GTGGACAGCT CCACTCGAGC GAAGGATTTC 
TGCAACAACA TCTCGCAGCG CCTTTCGCTG CGCACCAGCG AGGGCTTCTC CCTGTTTGTA 
AAGATCGCGG ATAAGGTCAT CTCTGTTCCC GAAGGTGATT TCTTCTTCGA TTTCGTACGC 
CATTTGACCG ACTGGATTAA AAAAGCGCGT CCCATACGAG ATGGAGCGAA TCCGCAGTTC 
ACTTACCAGG TCTTCTTCAT GAAGAAACTG TGGACGAACA CAGTGCCAGG CAAGGATCGG 
AATGCCGATC TTATATTCCA CTATCATCAG GAGTTGCCCA AGCTGTTGCG TGGCTATCAC 
AAGTGTTCCA GGGAGGAGGC GGCTAAGCTG GCCGCTCTAG TCTTCCGTGT GCGCTTTGGT 
GAAAATAAAC AAGAACTGCA GGCCATACCA CAAATGCTGA GGGAACTTAT TCCATCGGAT 
ATTATGAAAA TACAGAGCAC TAGCGAATGG AAACGATCGA TTGTTGCATC TTATAACCAG 
GATGGGGGCA TGACTTCCGA AGACGCGAAG GTGGCCTTCT TGAAGATTGT ATATAGATGG 
CCAACTTTTG GCTCCGCTTT CTTTGAGGTG AAGCAAACCA CCGAACCGAA TTATCCCGAA 
ATGCTATTGA TAGCCATTAA CAAACACGGC GTGAGTCTCA TACATCCAGT GACCAAGGAC 
ATTTTGGTTA CGCATCCATT TACACGCATC TCGAATTGGT CATCGGGCAA TACATATTTC 
CACATGACCA TCGGAAATCT GGTTAGGGGC TCGAAATTGT TATGTGAAAC GTCGCTGGGC 
TACAAGATGG ACGATCTGCT GACTTCATAT ATCTCGTTGA TGTTGACCAA TATGAACAAA 
AATCGAACCA TTCGAGCCAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523571.1
CDS	282..6785
Translation	MVIVTRGDYIWIEPASGREFDVAIGARVVSAEGRRIQVRDDDGDEVWLAPERRIKAMHASSVQGVEDMISLGDLHEAGILRNLLIRYKENLIYTYTGSILVAVNPYQILPIYTGDQIKLYKERKIGELPPHIFAIGDNAYAHMKRYRQDQCIVISGESGAGKTESTKLILQYLAAISGKHSWIEQQILEANPILEAFGNAKTIRNDNSSRFGKYIDIHFSANGVIEGAKIEQYLLEKSRIVSQNHSERNYHVFYCILAGLSADEKSRLDLGMAADYKYLTGGNSITCEGRDDAAEFSDIRSAMKVLLFSDQEIWEIIKLLAALLHCGNIKYKATVVDNLDATEIPEHINVERVAGLLGLPIQPLIDALTRRTLFAHGETVVSTLSRDQSVDVRDAFVKGIYGRMFVHIVRKINTAIFKPRGTSRNAIGVLDIFGFENFDQNSFEQFCINYANENLQQFFVQHIFKLEQEEYNHEAINWQHIEFVDNQDALDLIAIKQLNIMALIDEEARFPKGTDQTMLAKLHKTHGSHKNYLKPKSDINTSFGLNHFAGVVFYDTRGFLDKNRDTFSPDLLHLVSQSTNKFLRQIFAQDIEMGAETRKRTPTLSTQFRKSLDALMKTLSSCQPFFIRCIKPNELKKPMMFDRGLCCRQLRYSGMMETIRIRRAGYPIRHGFREFVERYRFLIPGVPPAHRTDCQAATSRICAVVLGKSDYQLGHTKVFLKDAHDLFLEQERDRVLTRKILILQRSIRGWVYRRRFLRLRAAAITVQRFWKGYAQRKRYRNMRVGYMRLQALIRSRVLSHRFRHLRGHIVGLQAHARGYLVRREYGHKMWAVIKIQSHVRRMIAMRRYRKLRLEHKQFAEVLQLRKLEEQELLHRGNKHAREIAEQHYRDRLHELERREIQEQLENRRRVEVNMNIINDAARKQEEPVDDGKLVEAMFDFLPDSSSDAPTPHGGRETSVFNDLPHAQNVNQDDIIAPIHISEDEEDLSEFKFQKFAATYFQGNVNHQYAKKALKHPLLPLHTQGDQLAAQALWITILRFTGDMPEPKYHTMDRMDTTSVMSKVTATLGRNFIRSKEFQEAQLMGLDPDAFLKQKPRSIRHKLVSLTLKRKNKLGEDVRRRLQDDEYTADSYQSWLQSRPTSNLEKLHFIIGHGILRAELRDEIYCQICKQLTNNPLKSSHARGWILLSLCVGCFAPSEKFVNYLRAFIREGPPGYAPYCEERLKRTFNNGTRNQPPSWLELQATKSKKPIMLPITFMDGNTKTLLADSATTARELCNQLSDKISLKDQFGFSLYIALFDKVSSLGSGGDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEIFAPWHEPTHDQVATNLIYQQVVRGVKFGEYRCDKEEDLAMIAAQQYFIEYSTDMSMERLFTLLPNFIPDFCLSGVDKAIERWAALVLQAYKKSYYVKDKIAPLKIKEDIVSYAKYKWPLLFSRFYEAYRNSGPNLPKNDVIIAVNWTGVYVVDDQEQVLLELSFPEITAVSSQKTNKVFTQTFSLSTVRGEEFTFQSPNAEDIRDLVVYFLDGLKKRSKYVIALQDYRAPSDGTSFLSFFKGDLIILEDESCGESVLNNGWCIGRCDRSQERGDFPAETVYVLPTLSKPPQDILALFNIEEAHHGRRLSMASNGGAVEPRDRPHTLMEYALDHFRLPPKRTMSKTLTLSSKRSEELWRYSRDPIKAPLLRKLQSKEEFAEEACFAFAAILKYMGDLPSKRPRMGNEITDHIFDGPLKHEILRDEIYCQLMKQLTDNRNRMSEERGWELMWLATGLFACSQGLLKELLLFLRTRRHPISQDSMHRLQKTIRHGQRKYPPHQVEVEAIQHKTTQIFHKVYFPDDTDEAFEVDSSTRAKDFCNNISQRLSLRTSEGFSLFVKIADKVISVPEGDFFFDFVRHLTDWIKKARPIRDGANPQFTYQVFFMKKLWTNTVPGKDRNADLIFHYHQELPKLLRGYHKCSREEAAKLAALVFRVRFGENKQELQAIPQMLRELIPSDIMKIQSTSEWKRSIVASYNQDGGMTSEDAKVAFLKIVYRWPTFGSAFFEVKQTTEPNYPEMLLIAINKHGVSLIHPVTKDILVTHPFTRISNWSSGNTYFHMTIGNLVRGSKLLCETSLGYKMDDLLTSYISLMLTNMNKNRTIRAN

Target ORF information:

RefSeq Version	NM_078847.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster crinkled (ck), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078847.4

ORF Insert Sequence:

ATGGTGATCG TAACACGTGG TGACTACATC TGGATAGAGC CCGCATCTGG GCGAGAATTC 
GATGTGGCCA TTGGAGCACG TGTCGTCTCC GCCGAGGGTC GTCGCATCCA GGTGCGCGAT 
GACGATGGCG ACGAGGTGTG GCTGGCACCC GAGCGCCGCA TCAAGGCCAT GCACGCGTCC 
TCCGTTCAGG GTGTGGAGGA CATGATATCC CTGGGTGATC TGCACGAGGC TGGCATCTTG 
CGGAATCTGC TCATCCGCTA CAAGGAGAAT CTGATATACA CTTACACGGG CTCCATACTT 
GTTGCCGTTA ATCCCTACCA GATTCTGCCC ATCTACACAG GTGATCAGAT CAAGCTCTAT 
AAGGAGCGGA AAATCGGTGA GCTACCGCCG CATATCTTTG CAATTGGCGA CAATGCGTAT 
GCGCATATGA AACGATATCG CCAGGATCAG TGTATCGTTA TTTCCGGAGA GTCTGGAGCT 
GGAAAGACGG AGAGCACAAA GTTGATCCTG CAGTATCTGG CTGCGATTAG TGGCAAACAC 
TCATGGATCG AACAGCAAAT CCTTGAAGCG AATCCCATTT TGGAAGCTTT CGGAAATGCC 
AAAACCATTC GAAATGATAA TTCATCGCGT TTTGGCAAAT ACATAGATAT ACACTTTAGC 
GCCAATGGCG TGATCGAGGG AGCCAAAATA GAACAGTACC TGCTGGAGAA GTCGCGAATT 
GTTTCCCAAA ATCATAGCGA ACGAAATTAT CATGTCTTTT ACTGCATTTT GGCCGGACTG 
TCGGCAGATG AGAAGAGTCG TCTCGATCTT GGCATGGCTG CTGATTACAA ATATCTGACT 
GGTGGTAATA GCATAACCTG CGAGGGACGC GATGACGCCG CCGAATTCTC TGACATTCGA 
TCTGCCATGA AGGTTTTGCT CTTCTCCGAT CAGGAAATCT GGGAGATAAT TAAACTCCTT 
GCTGCCCTTC TCCACTGCGG CAACATCAAA TATAAGGCGA CTGTGGTGGA TAATCTGGAT 
GCTACCGAAA TACCGGAACA CATAAATGTG GAGCGTGTGG CCGGGTTACT TGGACTTCCC 
ATTCAGCCGC TAATTGATGC TCTAACACGT CGAACACTTT TTGCTCATGG AGAAACCGTG 
GTGTCAACTC TGTCGCGCGA TCAATCCGTG GATGTGCGCG ATGCCTTTGT GAAAGGCATA 
TACGGACGTA TGTTTGTGCA CATCGTTCGA AAGATCAATA CGGCAATTTT CAAGCCGCGC 
GGCACATCCC GAAATGCCAT CGGAGTTCTC GACATCTTTG GTTTTGAGAA CTTCGATCAG 
AATAGCTTTG AGCAGTTTTG CATCAACTAT GCCAATGAGA ATCTGCAGCA GTTCTTCGTG 
CAGCATATAT TTAAACTTGA GCAGGAAGAG TATAACCACG AGGCCATCAA CTGGCAACAC 
ATTGAGTTCG TAGACAATCA GGATGCGCTC GACTTGATAG CTATTAAGCA GCTCAATATT 
ATGGCTTTAA TCGATGAGGA GGCCCGGTTT CCCAAGGGCA CGGATCAGAC GATGCTGGCC 
AAATTGCACA AAACCCATGG ATCGCACAAG AACTATTTGA AGCCCAAGTC GGATATCAAC 
ACCAGCTTTG GACTGAATCA CTTTGCCGGT GTGGTGTTCT ACGACACACG AGGATTTCTA 
GATAAAAACC GGGACACCTT CAGTCCCGAT CTATTGCATT TGGTTAGCCA AAGTACGAAC 
AAGTTCCTCC GACAAATCTT TGCTCAGGAC ATAGAGATGG GTGCAGAGAC GCGAAAGCGA 
ACACCCACAC TCTCCACACA GTTTCGAAAA TCTCTCGATG CGCTTATGAA GACGCTCAGC 
AGTTGCCAGC CATTCTTTAT TCGTTGTATC AAACCCAACG AGCTGAAGAA GCCAATGATG 
TTTGACCGCG GCTTGTGTTG CCGTCAGCTG CGATATTCCG GCATGATGGA AACGATCCGA 
ATCCGTCGCG CTGGATATCC CATTCGACAT GGATTCAGAG AGTTCGTCGA GCGTTATCGC 
TTCCTGATAC CGGGAGTTCC ACCAGCTCAT CGCACGGATT GCCAGGCGGC GACTTCGAGG 
ATTTGTGCCG TGGTTTTGGG CAAGTCCGAC TATCAGCTGG GCCACACCAA GGTCTTTCTC 
AAGGACGCCC ACGATCTGTT TCTGGAGCAG GAGAGGGATC GCGTGCTGAC GCGAAAAATC 
CTCATTCTGC AGCGCAGCAT TCGTGGTTGG GTGTACCGAC GAAGGTTTCT ACGCCTGAGA 
GCTGCTGCTA TCACTGTGCA GCGATTCTGG AAGGGCTATG CTCAACGTAA ACGATACAGG 
AACATGCGAG TGGGCTACAT GCGTTTGCAA GCGTTGATCC GTTCGAGAGT TCTGTCACAT 
CGTTTCCGCC ATCTGAGGGG CCACATTGTC GGGTTGCAAG CCCATGCCCG AGGATATTTG 
GTGAGGAGAG AGTATGGTCA CAAGATGTGG GCCGTCATTA AGATCCAGTC TCATGTGCGG 
CGTATGATTG CCATGCGGCG CTACCGAAAA CTTCGATTGG AGCACAAACA GTTTGCCGAA 
GTTCTTCAGC TGCGCAAACT GGAGGAGCAG GAACTGCTGC ATCGTGGCAA TAAGCATGCC 
CGAGAAATTG CCGAGCAGCA TTACCGAGAT CGATTGCACG AGCTGGAGCG TCGGGAAATT 
CAGGAGCAAT TGGAGAATCG TCGTCGAGTG GAGGTCAATA TGAATATTAT CAATGATGCA 
GCTCGCAAAC AGGAGGAGCC GGTGGACGAT GGCAAACTGG TGGAGGCCAT GTTTGACTTC 
CTTCCCGATT CCAGTAGCGA TGCCCCTACT CCGCATGGTG GACGTGAAAC GTCCGTGTTC 
AACGATCTGC CTCACGCGCA AAACGTCAAC CAGGACGATA TCATTGCACC CATACACATA 
TCCGAGGATG AGGAGGATCT ATCGGAATTC AAGTTCCAGA AATTCGCTGC CACCTACTTC 
CAAGGAAATG TCAATCATCA GTATGCTAAG AAAGCTTTGA AGCATCCCCT ACTTCCGCTC 
CATACGCAAG GCGATCAACT TGCTGCACAG GCATTGTGGA TAACCATACT GAGGTTTACA 
GGTGATATGC CGGAGCCGAA GTATCACACA ATGGATCGTA TGGACACCAC TTCGGTCATG 
TCCAAGGTCA CTGCCACCTT GGGGCGCAAT TTCATCAGGA GTAAGGAGTT CCAAGAGGCT 
CAGCTTATGG GCCTGGATCC AGATGCATTC CTTAAGCAAA AGCCACGATC GATCCGCCAC 
AAACTCGTAT CCCTGACGTT GAAGCGAAAG AACAAATTGG GCGAGGATGT GCGTCGCCGA 
CTGCAAGATG ATGAATACAC GGCGGATAGC TACCAATCGT GGCTACAATC CCGTCCAACC 
TCCAACCTTG AAAAGCTGCA TTTTATCATT GGCCATGGTA TCCTGCGTGC CGAGCTGCGT 
GACGAAATAT ACTGCCAGAT ATGCAAGCAG CTGACGAACA ATCCATTGAA ATCATCACAT 
GCCAGAGGTT GGATTCTGTT ATCCCTGTGC GTCGGATGTT TTGCTCCGTC GGAAAAGTTT 
GTTAACTACT TGAGGGCCTT TATCCGTGAG GGTCCACCTG GATATGCACC GTACTGCGAG 
GAGCGACTGA AGCGTACTTT CAACAATGGA ACTCGCAATC AACCGCCTTC GTGGCTGGAG 
TTGCAGGCCA CCAAATCCAA AAAGCCCATC ATGCTGCCCA TTACCTTTAT GGATGGAAAT 
ACAAAGACTC TGTTGGCTGA TTCCGCCACC ACGGCCCGAG AACTATGCAA TCAACTGTCC 
GACAAGATAA GTCTCAAAGA TCAGTTTGGT TTTTCCCTGT ACATAGCTCT GTTCGACAAA 
GTTAGTTCTT TGGGAAGTGG CGGGGACCAC GTGATGGACG CCATCTCACA ATGTGAGCAG 
TATGCAAAGG AGCAGGGCGC CCAGGAGCGG AATGCCCCGT GGCGTCTGTT TTTCCGCAAG 
GAAATCTTTG CACCTTGGCA CGAACCCACA CACGATCAGG TGGCCACCAA TCTTATTTAC 
CAGCAGGTGG TGAGAGGCGT TAAGTTTGGC GAATATCGCT GCGACAAGGA GGAAGACTTG 
GCCATGATAG CTGCGCAGCA ATATTTCATC GAATACTCAA CCGATATGTC AATGGAGCGG 
CTGTTTACCC TTCTGCCCAA CTTTATACCC GACTTTTGTC TCAGCGGAGT GGACAAGGCC 
ATTGAACGTT GGGCTGCTTT GGTTCTGCAG GCCTATAAAA AATCGTACTA TGTCAAGGAC 
AAGATCGCAC CCTTGAAAAT TAAGGAGGAT ATAGTTAGCT ACGCCAAGTA CAAGTGGCCT 
CTGCTCTTTT CTCGTTTCTA CGAAGCGTAT CGAAACTCCG GTCCGAATCT GCCCAAAAAC 
GATGTCATCA TTGCGGTGAA CTGGACCGGC GTGTATGTGG TTGATGATCA GGAGCAGGTG 
CTGTTGGAAC TGAGCTTCCC CGAGATTACT GCCGTGTCCA GTCAGAAGAC CAACAAGGTC 
TTCACCCAGA CTTTCAGCCT TTCCACTGTT CGAGGCGAGG AGTTCACCTT CCAGAGCCCC 
AATGCGGAGG ATATCCGTGA TCTGGTTGTC TACTTCTTAG ATGGCCTGAA GAAACGATCG 
AAATATGTCA TAGCCTTGCA GGACTATCGT GCGCCTTCGG ATGGAACAAG TTTTCTGTCT 
TTCTTTAAGG GAGATCTGAT CATACTAGAG GACGAATCCT GTGGAGAATC AGTACTGAAC 
AACGGCTGGT GCATAGGACG CTGCGATCGT TCCCAGGAGC GAGGAGACTT CCCCGCCGAA 
ACCGTGTACG TGCTGCCCAC CCTTAGCAAA CCGCCGCAGG ATATTTTGGC CCTGTTCAAC 
ATCGAGGAGG CGCATCATGG ACGACGTCTA AGCATGGCTT CCAATGGTGG AGCTGTGGAA 
CCAAGGGATC GACCTCATAC GCTGATGGAG TATGCCCTGG ATCACTTCCG TTTGCCACCG 
AAACGAACAA TGTCGAAAAC ACTCACTCTG AGTTCAAAAC GGAGTGAGGA ATTGTGGCGC 
TACTCCCGGG ATCCCATTAA GGCACCGCTG CTGCGCAAGT TGCAAAGCAA GGAAGAGTTT 
GCCGAGGAGG CCTGCTTCGC GTTTGCCGCC ATACTGAAAT ATATGGGCGA TCTGCCCTCC 
AAGCGTCCGC GGATGGGCAA CGAAATAACC GATCACATAT TTGATGGACC TCTGAAGCAC 
GAAATTCTGC GCGACGAGAT CTACTGCCAA CTGATGAAGC AGCTAACGGA CAATCGCAAC 
CGAATGTCCG AGGAGAGGGG CTGGGAGCTG ATGTGGTTGG CCACGGGACT GTTTGCCTGT 
TCACAGGGTT TGCTCAAGGA ATTGCTATTA TTCCTGCGCA CCAGGCGTCA TCCCATTTCA 
CAGGACTCAA TGCATCGACT ACAGAAAACT ATACGCCATG GACAACGCAA ATATCCGCCG 
CATCAGGTGG AGGTGGAAGC CATACAGCAT AAAACCACAC AGATCTTCCA CAAGGTCTAC 
TTCCCGGACG ACACAGACGA GGCCTTCGAA GTGGACAGCT CCACTCGAGC GAAGGATTTC 
TGCAACAACA TCTCGCAGCG CCTTTCGCTG CGCACCAGCG AGGGCTTCTC CCTGTTTGTA 
AAGATCGCGG ATAAGGTCAT CTCTGTTCCC GAAGGTGATT TCTTCTTCGA TTTCGTACGC 
CATTTGACCG ACTGGATTAA AAAAGCGCGT CCCATACGAG ATGGAGCGAA TCCGCAGTTC 
ACTTACCAGG TCTTCTTCAT GAAGAAACTG TGGACGAACA CAGTGCCAGG CAAGGATCGG 
AATGCCGATC TTATATTCCA CTATCATCAG GAGTTGCCCA AGCTGTTGCG TGGCTATCAC 
AAGTGTTCCA GGGAGGAGGC GGCTAAGCTG GCCGCTCTAG TCTTCCGTGT GCGCTTTGGT 
GAAAATAAAC AAGAACTGCA GGCCATACCA CAAATGCTGA GGGAACTTAT TCCATCGGAT 
ATTATGAAAA TACAGAGCAC TAGCGAATGG AAACGATCGA TTGTTGCATC TTATAACCAG 
GATGGGGGCA TGACTTCCGA AGACGCGAAG GTGGCCTTCT TGAAGATTGT ATATAGATGG 
CCAACTTTTG GCTCCGCTTT CTTTGAGGTG AAGCAAACCA CCGAACCGAA TTATCCCGAA 
ATGCTATTGA TAGCCATTAA CAAACACGGC GTGAGTCTCA TACATCCAGT GACCAAGGAC 
ATTTTGGTTA CGCATCCATT TACACGCATC TCGAATTGGT CATCGGGCAA TACATATTTC 
CACATGACCA TCGGAAATCT GGTTAGGGGC TCGAAATTGT TATGTGAAAC GTCGCTGGGC 
TACAAGATGG ACGATCTGCT GACTTCATAT ATCTCGTTGA TGTTGACCAA TATGAACAAA 
AATCGAACCA TTCGAGCCAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ck cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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