MYO7A gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017017781.1	XP_016873270.1	unconventional myosin-VIIa isoform X6
XM_017017785.1	XP_016873274.1	unconventional myosin-VIIa isoform X10
XM_017017786.1	XP_016873275.1	unconventional myosin-VIIa isoform X11
XM_017017787.1	XP_016873276.1	unconventional myosin-VIIa isoform X13
XM_017017788.1	XP_016873277.1	unconventional myosin-VIIa isoform X14
NM_001369365.1	NP_001356294.1	unconventional myosin-VIIa isoform 4
NM_001127180.1	NP_001120652.1	unconventional myosin-VIIa isoform 2
XM_017017779.1	XP_016873268.1	unconventional myosin-VIIa isoform X3
XM_017017784.1	XP_016873273.1	unconventional myosin-VIIa isoform X9
XM_011545050.2	XP_011543352.1	unconventional myosin-VIIa isoform X12
NM_000260.3	NP_000251.3	unconventional myosin-VIIa isoform 1
XM_017017778.1	XP_016873267.1	unconventional myosin-VIIa isoform X2
NM_000260.4	NP_000251.3	unconventional myosin-VIIa isoform 1
XM_011545046.2	XP_011543348.2	unconventional myosin-VIIa isoform X1
XM_017017780.1	XP_016873269.1	unconventional myosin-VIIa isoform X4
XM_011545044.2	XP_011543346.1	unconventional myosin-VIIa isoform X5
NM_001127179.2	NP_001120651.2	unconventional myosin-VIIa isoform 3
NM_001127180.2	NP_001120652.1	unconventional myosin-VIIa isoform 2
XM_017017782.1	XP_016873271.1	unconventional myosin-VIIa isoform X7
XM_017017783.1	XP_016873272.1	unconventional myosin-VIIa isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu92264	XM_017017787.1 Latest version!	Homo sapiens myosin VIIA (MYO7A), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu22132	NM_001127179.2 Latest version!	Homo sapiens myosin VIIA (MYO7A), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$832.30 $1189.00
OHu59159	XM_011545044.2 Latest version!	Homo sapiens myosin VIIA (MYO7A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22-24	$1091.30 $1559.00
OHu59165	XM_011545050.2 Latest version!	Homo sapiens myosin VIIA (MYO7A), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22-24	$1091.30 $1559.00
OHu108137	NM_001369365.1 Latest version!	Homo sapiens myosin VIIA (MYO7A), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu92254	XM_011545046.2 Latest version!	Homo sapiens myosin VIIA (MYO7A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu92255	XM_017017778.1 Latest version!	Homo sapiens myosin VIIA (MYO7A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu92256	XM_017017779.1 Latest version!	Homo sapiens myosin VIIA (MYO7A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu92257	XM_017017780.1 Latest version!	Homo sapiens myosin VIIA (MYO7A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu92258	XM_017017781.1 Latest version!	Homo sapiens myosin VIIA (MYO7A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu92259	XM_017017782.1 Latest version!	Homo sapiens myosin VIIA (MYO7A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu92260	XM_017017783.1 Latest version!	Homo sapiens myosin VIIA (MYO7A), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu92261	XM_017017784.1 Latest version!	Homo sapiens myosin VIIA (MYO7A), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu92262	XM_017017785.1 Latest version!	Homo sapiens myosin VIIA (MYO7A), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu92263	XM_017017786.1 Latest version!	Homo sapiens myosin VIIA (MYO7A), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu92265	XM_017017788.1 Latest version!	Homo sapiens myosin VIIA (MYO7A), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu26178	NM_001127180.2 Latest version!	Homo sapiens myosin VIIA (MYO7A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu27355	NM_000260.4 Latest version!	Homo sapiens myosin VIIA (MYO7A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
View Old Accession Versions >>
OHu27355	NM_000260.3	Homo sapiens myosin VIIA (MYO7A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu26178	NM_001127180.1	Homo sapiens myosin VIIA (MYO7A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
Hide Old Accession Versions >>

MYO7A ( XM_017017787.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873276.1 Homo sapiens myosin VIIA (MYO7A), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OHu92264
Clone ID Related Accession (Same CDS sequence)	XM_017017787.1
Accession Version	XM_017017787.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	unconventional myosin-VIIa isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACC CCGCCGACCC TGCCGACCCC GCGCGCATCC CGCAGCCCGG CAAGTGGGCG 
CTGGTGCGCA GCCTGAACCA GGCGTTCAAG ACCTACGCCG TCCTGCCGGG GGACCATGTG 
TGGATGGACC TGAGATTGGG GCAGGAGTTC GACGTGCCCA TCGGGGCGGT GGTGAAGCTC 
TGCGACTCTG GGCAGGTCCA GGTGGTGGAT GATGAAGACA ATGAACACTG GATCTCTCCG 
CAGAACGCAA CGCACATCAA GCCTATGCAC CCCACGTCGG TCCACGGCGT GGAGGACATG 
ATCCGCCTGG GGGACCTCAA CGAGGCGGGC ATCTTGCGCA ACCTGCTTAT CCGCTACCGG 
GACCACCTCA TCTACACGTA TACGGGCTCC ATCCTGGTGG CTGTGAACCC CTACCAGCTG 
CTCTCCATCT ACTCGCCAGA GCACATCCGC CAGTATACCA ACAAGAAGAT TGGGGAGATG 
CCCCCCCACA TCTTTGCCAT TGCTGACAAC TGCTACTTCA ACATGAAACG CAACAGCCGA 
GACCAGTGCT GCATCATCAG TGGGGAATCT GGGGCCGGGA AGACGGAGAG CACAAAGCTG 
ATCCTGCAGT TCCTGGCAGC CATCAGTGGG CAGCACTCGT GGATTGAGCA GCAGGTCTTG 
GAGGCCACCC CCATTCTGGA AGCATTTGGG AATGCCAAGA CCATCCGCAA TGACAACTCA 
AGCCGTTTCG GAAAGTACAT CGACATCCAC TTCAACAAGC GGGGCGCCAT CGAGGGCGCG 
AAGATTGAGC AGTACCTGCT GGAAAAGTCA CGTGTCTGTC GCCAGGCCCT GGATGAAAGG 
AACTACCACG TGTTCTACTG CATGCTGGAG GGTATGAGTG AGGATCAGAA GAAGAAGCTG 
GGCTTGGGCC AGGCCTCTGA CTACAACTAC TTGGCCATGG GTAACTGCAT AACCTGTGAG 
GGCCGGGTGG ACAGCCAGGA GTACGCCAAC ATCCGCTCCG CCATGAAGGT GCTCATGTTC 
ACTGACACCG AGAACTGGGA GATCTCGAAG CTCCTGGCTG CCATCCTGCA CCTGGGCAAC 
CTGCAGTATG AGGCACGCAC ATTTGAAAAC CTGGATGCCT GTGAGGTTCT CTTCTCCCCA 
TCGCTGGCCA CAGCTGCATC CCTGCTTGAG GTGAACCCCC CAGACCTGAT GAGCTGCCTG 
ACTAGCCGCA CCCTCATCAC CCGCGGGGAG ACGGTGTCCA CCCCACTGAG CAGGGAACAG 
GCACTGGACG TGCGCGACGC CTTCGTAAAG GGGATCTACG GGCGGCTGTT CGTGTGGATT 
GTGGACAAGA TCAACGCAGC AATTTACAAG CCTCCCTCCC AGGATGTGAA GAACTCTCGC 
AGGTCCATCG GCCTCCTGGA CATCTTTGGG TTTGAGAACT TTGCTGTGAA CAGCTTTGAG 
CAGCTCTGCA TCAACTTCGC CAATGAGCAC CTGCAGCAGT TCTTTGTGCG GCACGTGTTC 
AAGCTGGAGC AGGAGGAATA TGACCTGGAG AGCATTGACT GGCTGCACAT CGAGTTCACT 
GACAACCAGG ATGCCCTGGA CATGATTGCC AACAAGCCCA TGAACATCAT CTCCCTCATC 
GATGAGGAGA GCAAGTTCCC CAAGGGCACA GACACCACCA TGTTACACAA GCTGAACTCC 
CAGCACAAGC TCAACGCCAA CTACATCCCC CCCAAGAACA ACCATGAGAC CCAGTTTGGC 
ATCAACCATT TTGCAGGCAT CGTCTACTAT GAGACCCAAG GCTTCCTGGA GAAGAACCGA 
GACACCCTGC ATGGGGACAT TATCCAGCTG GTCCACTCCT CCAGGAACAA GTTCATCAAG 
CAGATCTTCC AGGCCGATGT CGCCATGGGC GCCGAGACCA GGAAGCGCTC GCCCACACTT 
AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG CTGATGCGCA CGCTGGGTGC CTGCCAGCCC 
TTCTTTGTGC GATGCATCAA GCCCAATGAG TTCAAGAAGC CCATGCTGTT CGACCGGCAC 
CTGTGCGTGC GCCAGCTGCG GTACTCAGGA ATGATGGAGA CCATCCGAAT CCGCCGAGCT 
GGCTACCCCA TCCGCTACAG CTTCGTAGAG TTTGTGGAGC GGTACCGTGT GCTGCTGCCA 
GGTGTGAAGC CGGCCTACAA GCAGGGCGAC CTCCGCGGGA CTTGCCAGCG CATGGCTGAG 
GCTGTGCTGG GCACCCACGA TGACTGGCAG ATAGGCAAAA CCAAGATCTT TCTGAAGGAC 
CACCATGACA TGCTGCTGGA AGTGGAGCGG GACAAAGCCA TCACCGACAG AGTCATCCTC 
CTTCAGAAAG TCATCCGGGG ATTCAAAGAC AGGTCTAACT TTCTGAAGCT GAAGAACGCT 
GCCACACTGA TCCAGAGGCA CTGGCGGGGT CACAACTGTA GGAAGAACTA CGGGCTGATG 
CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG CACCGCTCCC GGAAGCTGCA CCAGCAGTAC 
CGCCTGGCCC GCCAGCGCAT CATCCAGTTC CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC 
AAGGCCTTCC GCCACCGCCT CTGGGCTGTG CTCACCGTGC AGGCCTATGC CCGGGGCATG 
ATCGCCCGCA GGCTGCACCA ACGCCTCAGG GCTGAGTATC TGTGGCGCCT CGAGGCTGAG 
AAAATGCGGC TGGCGGAGGA AGAGAAGCTT CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG 
GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC CTGGCCCAGC TGGCTCGTGA GGACGCTGAG 
CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG CGGAAGAAGG AGCTCCTGGA GCAGATGGAA 
AGGGCCCGCC ATGAGCCTGT CAATCACTCA GACATGGTGG ACAAGATGTT TGGCTTCCTG 
GGGACTTCAG GTGGCCTGCC AGGCCAGGAG GGCCAGGCAC CTAGTGGCTT TGAGGACCTG 
GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG GACCTGGATG CAGCCCTGCC CCTGCCTGAC 
GAGGATGAGG AGGACCTCTC TGAGTATAAA TTTGCCAAGT TCGCGGCCAC CTACTTCCAG 
GGGACAACCA CGCACTCCTA CACCCGGCGG CCACTCAAAC AGCCACTGCT CTACCATGAC 
GACGAGGGTG ACCAGCTGGC AGCCCTGGCG GTCTGGATCA CCATCCTCCG CTTCATGGGG 
GACCTCCCTG AGCCCAAGTA CCACACAGCC ATGAGTGATG GCAGTGAGAA GATCCCTGTG 
ATGACCAAGA TTTATGAGAC CCTGGGCAAG AAGACGTACA AGAGGGAGCT GCAGGCCCTG 
CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG GGCCAGAAGA AGAGCAGTGT GAGGCACAAG 
CTGGTGCATT TGACTCTGAA AAAGAAGTCC AAGCTCACAG AGGAGGTGAC CAAGAGGCTG 
CATGACGGGG AGTCCACAGT GCAGGGCAAC AGCATGCTGG AGGACCGGCC CACCTCCAAC 
CTGGAGAAGC TGCACTTCAT CATCGGCAAT GGCATCCTGC GGCCAGCACT CCGGGACGAG 
ATCTACTGCC AGATCAGCAA GCAGCTGACC CACAACCCCT CCAAGAGCAG CTATGCCCGG 
GGCTGGATTC TCGTGTCTCT CTGCGTGGGC TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG 
TACCTGCGGA ACTTCATCCA CGGGGGCCCG CCCGGCTACG CCCCGTACTG TGAGGAGCGC 
CTGAGAAGGA CCTTTGTCAA TGGGACACGG ACACAGCCGC CCAGCTGGCT GGAGCTGCAG 
GCCACCAAGT CCAAGAAGCC AATCATGTTG CCCGTGACAT TCATGGATGG GACCACCAAG 
ACCCTGCTGA CGGACTCGGC AACCACGGCC AAGGAGCTCT GCAACGCGCT GGCCGACAAG 
ATCTCTCTCA AGGACCGGTT CGGGTTCTCC CTCTACATTG CCCTGTTTGA CAAGCAACGT 
CATGCTCAGC AGAGGGGCCC CCGCCCACAA GCCCAGGGGA GGTACCGTGT TGCTGGACAC 
AGTTTGCCTG GATTTTTCTC ACCGATGCTC TTATGTTTCC AGGTGTCCTC CCTGGGCAGC 
GGCAGTGACC ACGTCATGGA CGCCATCTCC CAGTGCGAGC AGTACGCCAA GGAGCAGGGC 
GCCCAGGAGC GCAACGCCCC CTGGAGGCTC TTCTTCCGCA AAGAGGTCTT CACGCCCTGG 
CACAGCCCCT CCGAGGACAA CGTGGCCACC AACCTCATCT ACCAGCAGGT GGTGCGAGGA 
GTCAAGTTTG GGGAGTACAG GTGTGAGAAG GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873276.1
CDS	16..4371
Translation	MEDPADPADPARIPQPGKWALVRSLNQAFKTYAVLPGDHVWMDLRLGQEFDVPIGAVVKLCDSGQVQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNSRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQALDERNYHVFYCMLEGMSEDQKKKLGLGQASDYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHLGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVDKINAAIYKPPSQDVKNSRRSIGLLDIFGFENFAVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQDALDMIANKPMNIISLIDEESKFPKGTDTTMLHKLNSQHKLNANYIPPKNNHETQFGINHFAGIVYYETQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRRAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLKLKNAATLIQRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLHQQYRLARQRIIQFQARCRAYLVRKAFRHRLWAVLTVQAYARGMIARRLHQRLRAEYLWRLEAEKMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEAARRKKELLEQMERARHEPVNHSDMVDKMFGFLGTSGGLPGQEGQAPSGFEDLERGRREMVEEDLDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGEAQLPEGQKKSSVRHKLVHLTLKKKSKLTEEVTKRLHDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTAKELCNALADKISLKDRFGFSLYIALFDKQRHAQQRGPRPQAQGRYRVAGHSLPGFFSPMLLCFQVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHSPSEDNVATNLIYQQVVRGVKFGEYRCEKG

Target ORF information:

RefSeq Version	XM_017017787.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VIIA (MYO7A), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017787.1

ORF Insert Sequence:

ATGGAGGACC CCGCCGACCC TGCCGACCCC GCGCGCATCC CGCAGCCCGG CAAGTGGGCG 
CTGGTGCGCA GCCTGAACCA GGCGTTCAAG ACCTACGCCG TCCTGCCGGG GGACCATGTG 
TGGATGGACC TGAGATTGGG GCAGGAGTTC GACGTGCCCA TCGGGGCGGT GGTGAAGCTC 
TGCGACTCTG GGCAGGTCCA GGTGGTGGAT GATGAAGACA ATGAACACTG GATCTCTCCG 
CAGAACGCAA CGCACATCAA GCCTATGCAC CCCACGTCGG TCCACGGCGT GGAGGACATG 
ATCCGCCTGG GGGACCTCAA CGAGGCGGGC ATCTTGCGCA ACCTGCTTAT CCGCTACCGG 
GACCACCTCA TCTACACGTA TACGGGCTCC ATCCTGGTGG CTGTGAACCC CTACCAGCTG 
CTCTCCATCT ACTCGCCAGA GCACATCCGC CAGTATACCA ACAAGAAGAT TGGGGAGATG 
CCCCCCCACA TCTTTGCCAT TGCTGACAAC TGCTACTTCA ACATGAAACG CAACAGCCGA 
GACCAGTGCT GCATCATCAG TGGGGAATCT GGGGCCGGGA AGACGGAGAG CACAAAGCTG 
ATCCTGCAGT TCCTGGCAGC CATCAGTGGG CAGCACTCGT GGATTGAGCA GCAGGTCTTG 
GAGGCCACCC CCATTCTGGA AGCATTTGGG AATGCCAAGA CCATCCGCAA TGACAACTCA 
AGCCGTTTCG GAAAGTACAT CGACATCCAC TTCAACAAGC GGGGCGCCAT CGAGGGCGCG 
AAGATTGAGC AGTACCTGCT GGAAAAGTCA CGTGTCTGTC GCCAGGCCCT GGATGAAAGG 
AACTACCACG TGTTCTACTG CATGCTGGAG GGTATGAGTG AGGATCAGAA GAAGAAGCTG 
GGCTTGGGCC AGGCCTCTGA CTACAACTAC TTGGCCATGG GTAACTGCAT AACCTGTGAG 
GGCCGGGTGG ACAGCCAGGA GTACGCCAAC ATCCGCTCCG CCATGAAGGT GCTCATGTTC 
ACTGACACCG AGAACTGGGA GATCTCGAAG CTCCTGGCTG CCATCCTGCA CCTGGGCAAC 
CTGCAGTATG AGGCACGCAC ATTTGAAAAC CTGGATGCCT GTGAGGTTCT CTTCTCCCCA 
TCGCTGGCCA CAGCTGCATC CCTGCTTGAG GTGAACCCCC CAGACCTGAT GAGCTGCCTG 
ACTAGCCGCA CCCTCATCAC CCGCGGGGAG ACGGTGTCCA CCCCACTGAG CAGGGAACAG 
GCACTGGACG TGCGCGACGC CTTCGTAAAG GGGATCTACG GGCGGCTGTT CGTGTGGATT 
GTGGACAAGA TCAACGCAGC AATTTACAAG CCTCCCTCCC AGGATGTGAA GAACTCTCGC 
AGGTCCATCG GCCTCCTGGA CATCTTTGGG TTTGAGAACT TTGCTGTGAA CAGCTTTGAG 
CAGCTCTGCA TCAACTTCGC CAATGAGCAC CTGCAGCAGT TCTTTGTGCG GCACGTGTTC 
AAGCTGGAGC AGGAGGAATA TGACCTGGAG AGCATTGACT GGCTGCACAT CGAGTTCACT 
GACAACCAGG ATGCCCTGGA CATGATTGCC AACAAGCCCA TGAACATCAT CTCCCTCATC 
GATGAGGAGA GCAAGTTCCC CAAGGGCACA GACACCACCA TGTTACACAA GCTGAACTCC 
CAGCACAAGC TCAACGCCAA CTACATCCCC CCCAAGAACA ACCATGAGAC CCAGTTTGGC 
ATCAACCATT TTGCAGGCAT CGTCTACTAT GAGACCCAAG GCTTCCTGGA GAAGAACCGA 
GACACCCTGC ATGGGGACAT TATCCAGCTG GTCCACTCCT CCAGGAACAA GTTCATCAAG 
CAGATCTTCC AGGCCGATGT CGCCATGGGC GCCGAGACCA GGAAGCGCTC GCCCACACTT 
AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG CTGATGCGCA CGCTGGGTGC CTGCCAGCCC 
TTCTTTGTGC GATGCATCAA GCCCAATGAG TTCAAGAAGC CCATGCTGTT CGACCGGCAC 
CTGTGCGTGC GCCAGCTGCG GTACTCAGGA ATGATGGAGA CCATCCGAAT CCGCCGAGCT 
GGCTACCCCA TCCGCTACAG CTTCGTAGAG TTTGTGGAGC GGTACCGTGT GCTGCTGCCA 
GGTGTGAAGC CGGCCTACAA GCAGGGCGAC CTCCGCGGGA CTTGCCAGCG CATGGCTGAG 
GCTGTGCTGG GCACCCACGA TGACTGGCAG ATAGGCAAAA CCAAGATCTT TCTGAAGGAC 
CACCATGACA TGCTGCTGGA AGTGGAGCGG GACAAAGCCA TCACCGACAG AGTCATCCTC 
CTTCAGAAAG TCATCCGGGG ATTCAAAGAC AGGTCTAACT TTCTGAAGCT GAAGAACGCT 
GCCACACTGA TCCAGAGGCA CTGGCGGGGT CACAACTGTA GGAAGAACTA CGGGCTGATG 
CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG CACCGCTCCC GGAAGCTGCA CCAGCAGTAC 
CGCCTGGCCC GCCAGCGCAT CATCCAGTTC CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC 
AAGGCCTTCC GCCACCGCCT CTGGGCTGTG CTCACCGTGC AGGCCTATGC CCGGGGCATG 
ATCGCCCGCA GGCTGCACCA ACGCCTCAGG GCTGAGTATC TGTGGCGCCT CGAGGCTGAG 
AAAATGCGGC TGGCGGAGGA AGAGAAGCTT CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG 
GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC CTGGCCCAGC TGGCTCGTGA GGACGCTGAG 
CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG CGGAAGAAGG AGCTCCTGGA GCAGATGGAA 
AGGGCCCGCC ATGAGCCTGT CAATCACTCA GACATGGTGG ACAAGATGTT TGGCTTCCTG 
GGGACTTCAG GTGGCCTGCC AGGCCAGGAG GGCCAGGCAC CTAGTGGCTT TGAGGACCTG 
GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG GACCTGGATG CAGCCCTGCC CCTGCCTGAC 
GAGGATGAGG AGGACCTCTC TGAGTATAAA TTTGCCAAGT TCGCGGCCAC CTACTTCCAG 
GGGACAACCA CGCACTCCTA CACCCGGCGG CCACTCAAAC AGCCACTGCT CTACCATGAC 
GACGAGGGTG ACCAGCTGGC AGCCCTGGCG GTCTGGATCA CCATCCTCCG CTTCATGGGG 
GACCTCCCTG AGCCCAAGTA CCACACAGCC ATGAGTGATG GCAGTGAGAA GATCCCTGTG 
ATGACCAAGA TTTATGAGAC CCTGGGCAAG AAGACGTACA AGAGGGAGCT GCAGGCCCTG 
CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG GGCCAGAAGA AGAGCAGTGT GAGGCACAAG 
CTGGTGCATT TGACTCTGAA AAAGAAGTCC AAGCTCACAG AGGAGGTGAC CAAGAGGCTG 
CATGACGGGG AGTCCACAGT GCAGGGCAAC AGCATGCTGG AGGACCGGCC CACCTCCAAC 
CTGGAGAAGC TGCACTTCAT CATCGGCAAT GGCATCCTGC GGCCAGCACT CCGGGACGAG 
ATCTACTGCC AGATCAGCAA GCAGCTGACC CACAACCCCT CCAAGAGCAG CTATGCCCGG 
GGCTGGATTC TCGTGTCTCT CTGCGTGGGC TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG 
TACCTGCGGA ACTTCATCCA CGGGGGCCCG CCCGGCTACG CCCCGTACTG TGAGGAGCGC 
CTGAGAAGGA CCTTTGTCAA TGGGACACGG ACACAGCCGC CCAGCTGGCT GGAGCTGCAG 
GCCACCAAGT CCAAGAAGCC AATCATGTTG CCCGTGACAT TCATGGATGG GACCACCAAG 
ACCCTGCTGA CGGACTCGGC AACCACGGCC AAGGAGCTCT GCAACGCGCT GGCCGACAAG 
ATCTCTCTCA AGGACCGGTT CGGGTTCTCC CTCTACATTG CCCTGTTTGA CAAGCAACGT 
CATGCTCAGC AGAGGGGCCC CCGCCCACAA GCCCAGGGGA GGTACCGTGT TGCTGGACAC 
AGTTTGCCTG GATTTTTCTC ACCGATGCTC TTATGTTTCC AGGTGTCCTC CCTGGGCAGC 
GGCAGTGACC ACGTCATGGA CGCCATCTCC CAGTGCGAGC AGTACGCCAA GGAGCAGGGC 
GCCCAGGAGC GCAACGCCCC CTGGAGGCTC TTCTTCCGCA AAGAGGTCTT CACGCCCTGG 
CACAGCCCCT CCGAGGACAA CGTGGCCACC AACCTCATCT ACCAGCAGGT GGTGCGAGGA 
GTCAAGTTTG GGGAGTACAG GTGTGAGAAG GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO7A ( NM_001127179.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001120651.2 Homo sapiens myosin VIIA (MYO7A), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu22132
Clone ID Related Accession (Same CDS sequence)	NM_001127179.2
Accession Version	NM_001127179.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-14
Organism	Homo sapiens(Human)
Product	unconventional myosin-VIIa isoform 3
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U39226.1, U55209.1, AP000752.4 and BC023212.1. On Aug 21, 2009 this sequence version replaced NM_001127179.1. Transcript Variant: This variant (3) differs in the 3' coding region, compared to variant 1. It encodes isoform 3 which is shorter and has a distinct C-terminus compared to isoform 1. Sequence Note: Version 1 of this RefSeq record represented an alternate haplotype with a 22-nt gap in the terminal exon. NM_001127179.2 reflects an update to represent the haplotype found in the reference genome, which also appears to be the ancestral allele, and encodes a protein with a shorter and distinct C-terminus than found in version 1. This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGATTC TTCAGCAGGG GGACCATGTG TGGATGGACC TGAGATTGGG GCAGGAGTTC 
GACGTGCCCA TCGGGGCGGT GGTGAAGCTC TGCGACTCTG GGCAGGTCCA GGTGGTGGAT 
GATGAAGACA ATGAACACTG GATCTCTCCG CAGAACGCAA CGCACATCAA GCCTATGCAC 
CCCACGTCGG TCCACGGCGT GGAGGACATG ATCCGCCTGG GGGACCTCAA CGAGGCGGGC 
ATCTTGCGCA ACCTGCTTAT CCGCTACCGG GACCACCTCA TCTACACGTA TACGGGCTCC 
ATCCTGGTGG CTGTGAACCC CTACCAGCTG CTCTCCATCT ACTCGCCAGA GCACATCCGC 
CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAC 
TGCTACTTCA ACATGAAACG CAACAGCCGA GACCAGTGCT GCATCATCAG TGGGGAATCT 
GGGGCCGGGA AGACGGAGAG CACAAAGCTG ATCCTGCAGT TCCTGGCAGC CATCAGTGGG 
CAGCACTCGT GGATTGAGCA GCAGGTCTTG GAGGCCACCC CCATTCTGGA AGCATTTGGG 
AATGCCAAGA CCATCCGCAA TGACAACTCA AGCCGTTTCG GAAAGTACAT CGACATCCAC 
TTCAACAAGC GGGGCGCCAT CGAGGGCGCG AAGATTGAGC AGTACCTGCT GGAAAAGTCA 
CGTGTCTGTC GCCAGGCCCT GGATGAAAGG AACTACCACG TGTTCTACTG CATGCTGGAG 
GGTATGAGTG AGGATCAGAA GAAGAAGCTG GGCTTGGGCC AGGCCTCTGA CTACAACTAC 
TTGGCCATGG GTAACTGCAT AACCTGTGAG GGCCGGGTGG ACAGCCAGGA GTACGCCAAC 
ATCCGCTCCG CCATGAAGGT GCTCATGTTC ACTGACACCG AGAACTGGGA GATCTCGAAG 
CTCCTGGCTG CCATCCTGCA CCTGGGCAAC CTGCAGTATG AGGCACGCAC ATTTGAAAAC 
CTGGATGCCT GTGAGGTTCT CTTCTCCCCA TCGCTGGCCA CAGCTGCATC CCTGCTTGAG 
GTGAACCCCC CAGACCTGAT GAGCTGCCTG ACTAGCCGCA CCCTCATCAC CCGCGGGGAG 
ACGGTGTCCA CCCCACTGAG CAGGGAACAG GCACTGGACG TGCGCGACGC CTTCGTAAAG 
GGGATCTACG GGCGGCTGTT CGTGTGGATT GTGGACAAGA TCAACGCAGC AATTTACAAG 
CCTCCCTCCC AGGATGTGAA GAACTCTCGC AGGTCCATCG GCCTCCTGGA CATCTTTGGG 
TTTGAGAACT TTGCTGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTCGC CAATGAGCAC 
CTGCAGCAGT TCTTTGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAATA TGACCTGGAG 
AGCATTGACT GGCTGCACAT CGAGTTCACT GACAACCAGG ATGCCCTGGA CATGATTGCC 
AACAAGCCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACA 
GACACCACCA TGTTACACAA GCTGAACTCC CAGCACAAGC TCAACGCCAA CTACATCCCC 
CCCAAGAACA ACCATGAGAC CCAGTTTGGC ATCAACCATT TTGCAGGCAT CGTCTACTAT 
GAGACCCAAG GCTTCCTGGA GAAGAACCGA GACACCCTGC ATGGGGACAT TATCCAGCTG 
GTCCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT CGCCATGGGC 
GCCGAGACCA GGAAGCGCTC GCCCACACTT AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG 
CTGATGCGCA CGCTGGGTGC CTGCCAGCCC TTCTTTGTGC GATGCATCAA GCCCAATGAG 
TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGCGTGC GCCAGCTGCG GTACTCAGGA 
ATGATGGAGA CCATCCGAAT CCGCCGAGCT GGCTACCCCA TCCGCTACAG CTTCGTAGAG 
TTTGTGGAGC GGTACCGTGT GCTGCTGCCA GGTGTGAAGC CGGCCTACAA GCAGGGCGAC 
CTCCGCGGGA CTTGCCAGCG CATGGCTGAG GCTGTGCTGG GCACCCACGA TGACTGGCAG 
ATAGGCAAAA CCAAGATCTT TCTGAAGGAC CACCATGACA TGCTGCTGGA AGTGGAGCGG 
GACAAAGCCA TCACCGACAG AGTCATCCTC CTTCAGAAAG TCATCCGGGG ATTCAAAGAC 
AGGTCTAACT TTCTGAAGCT GAAGAACGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGT 
CACAACTGTA GGAAGAACTA CGGGCTGATG CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG 
CACCGCTCCC GGAAGCTGCA CCAGCAGTAC CGCCTGGCCC GCCAGCGCAT CATCCAGTTC 
CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC AAGGCCTTCC GCCACCGCCT CTGGGCTGTG 
CTCACCGTGC AGGCCTATGC CCGGGGCATG ATCGCCCGCA GGCTGCACCA ACGCCTCAGG 
GCTGAGTATC TGTGGCGCCT CGAGGCTGAG AAAATGCGGC TGGCGGAGGA AGAGAAGCTT 
CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC 
CTGGCCCAGC TGGCTCGTGA GGACGCTGAG CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG 
CGGAAGAAGG AGCTCCTGGA GCAGATGGAA AGGGCCCGCC ATGAGCCTGT CAATCACTCA 
GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCAG GTGGCCTGCC AGGCCAGGAG 
GGCCAGGCAC CTAGTGGCTT TGAGGACCTG GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG 
GACCTGGATG CAGCCCTGCC CCTGCCTGAC GAGGATGAGG AGGACCTCTC TGAGTATAAA 
TTTGCCAAGT TCGCGGCCAC CTACTTCCAG GGGACAACCA CGCACTCCTA CACCCGGCGG 
CCACTCAAAC AGCCACTGCT CTACCATGAC GACGAGGGTG ACCAGCTGGC AGCCCTGGCG 
GTCTGGATCA CCATCCTCCG CTTCATGGGG GACCTCCCTG AGCCCAAGTA CCACACAGCC 
ATGAGTGATG GCAGTGAGAA GATCCCTGTG ATGACCAAGA TTTATGAGAC CCTGGGCAAG 
AAGACGTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG 
GGCCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCATT TGACTCTGAA AAAGAAGTCC 
AAGCTCACAG AGGAGGTGAC CAAGAGGCTG CATGACGGGG AGTCCACAGT GCAGGGCAAC 
AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATCGGCAAT 
GGCATCCTGC GGCCAGCACT CCGGTCAGTG CCGGGAGGCG GGGACACCAG GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120651.2
CDS	273..3809
Translation	MVILQQGDHVWMDLRLGQEFDVPIGAVVKLCDSGQVQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNSRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQALDERNYHVFYCMLEGMSEDQKKKLGLGQASDYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHLGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVDKINAAIYKPPSQDVKNSRRSIGLLDIFGFENFAVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQDALDMIANKPMNIISLIDEESKFPKGTDTTMLHKLNSQHKLNANYIPPKNNHETQFGINHFAGIVYYETQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRRAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLKLKNAATLIQRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLHQQYRLARQRIIQFQARCRAYLVRKAFRHRLWAVLTVQAYARGMIARRLHQRLRAEYLWRLEAEKMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEAARRKKELLEQMERARHEPVNHSDMVDKMFGFLGTSGGLPGQEGQAPSGFEDLERGRREMVEEDLDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGEAQLPEGQKKSSVRHKLVHLTLKKKSKLTEEVTKRLHDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRSVPGGGDTRA

Target ORF information:

RefSeq Version	NM_001127179.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens myosin VIIA (MYO7A), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127179.2

ORF Insert Sequence:

ATGGTGATTC TTCAGCAGGG GGACCATGTG TGGATGGACC TGAGATTGGG GCAGGAGTTC 
GACGTGCCCA TCGGGGCGGT GGTGAAGCTC TGCGACTCTG GGCAGGTCCA GGTGGTGGAT 
GATGAAGACA ATGAACACTG GATCTCTCCG CAGAACGCAA CGCACATCAA GCCTATGCAC 
CCCACGTCGG TCCACGGCGT GGAGGACATG ATCCGCCTGG GGGACCTCAA CGAGGCGGGC 
ATCTTGCGCA ACCTGCTTAT CCGCTACCGG GACCACCTCA TCTACACGTA TACGGGCTCC 
ATCCTGGTGG CTGTGAACCC CTACCAGCTG CTCTCCATCT ACTCGCCAGA GCACATCCGC 
CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAC 
TGCTACTTCA ACATGAAACG CAACAGCCGA GACCAGTGCT GCATCATCAG TGGGGAATCT 
GGGGCCGGGA AGACGGAGAG CACAAAGCTG ATCCTGCAGT TCCTGGCAGC CATCAGTGGG 
CAGCACTCGT GGATTGAGCA GCAGGTCTTG GAGGCCACCC CCATTCTGGA AGCATTTGGG 
AATGCCAAGA CCATCCGCAA TGACAACTCA AGCCGTTTCG GAAAGTACAT CGACATCCAC 
TTCAACAAGC GGGGCGCCAT CGAGGGCGCG AAGATTGAGC AGTACCTGCT GGAAAAGTCA 
CGTGTCTGTC GCCAGGCCCT GGATGAAAGG AACTACCACG TGTTCTACTG CATGCTGGAG 
GGTATGAGTG AGGATCAGAA GAAGAAGCTG GGCTTGGGCC AGGCCTCTGA CTACAACTAC 
TTGGCCATGG GTAACTGCAT AACCTGTGAG GGCCGGGTGG ACAGCCAGGA GTACGCCAAC 
ATCCGCTCCG CCATGAAGGT GCTCATGTTC ACTGACACCG AGAACTGGGA GATCTCGAAG 
CTCCTGGCTG CCATCCTGCA CCTGGGCAAC CTGCAGTATG AGGCACGCAC ATTTGAAAAC 
CTGGATGCCT GTGAGGTTCT CTTCTCCCCA TCGCTGGCCA CAGCTGCATC CCTGCTTGAG 
GTGAACCCCC CAGACCTGAT GAGCTGCCTG ACTAGCCGCA CCCTCATCAC CCGCGGGGAG 
ACGGTGTCCA CCCCACTGAG CAGGGAACAG GCACTGGACG TGCGCGACGC CTTCGTAAAG 
GGGATCTACG GGCGGCTGTT CGTGTGGATT GTGGACAAGA TCAACGCAGC AATTTACAAG 
CCTCCCTCCC AGGATGTGAA GAACTCTCGC AGGTCCATCG GCCTCCTGGA CATCTTTGGG 
TTTGAGAACT TTGCTGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTCGC CAATGAGCAC 
CTGCAGCAGT TCTTTGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAATA TGACCTGGAG 
AGCATTGACT GGCTGCACAT CGAGTTCACT GACAACCAGG ATGCCCTGGA CATGATTGCC 
AACAAGCCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACA 
GACACCACCA TGTTACACAA GCTGAACTCC CAGCACAAGC TCAACGCCAA CTACATCCCC 
CCCAAGAACA ACCATGAGAC CCAGTTTGGC ATCAACCATT TTGCAGGCAT CGTCTACTAT 
GAGACCCAAG GCTTCCTGGA GAAGAACCGA GACACCCTGC ATGGGGACAT TATCCAGCTG 
GTCCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT CGCCATGGGC 
GCCGAGACCA GGAAGCGCTC GCCCACACTT AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG 
CTGATGCGCA CGCTGGGTGC CTGCCAGCCC TTCTTTGTGC GATGCATCAA GCCCAATGAG 
TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGCGTGC GCCAGCTGCG GTACTCAGGA 
ATGATGGAGA CCATCCGAAT CCGCCGAGCT GGCTACCCCA TCCGCTACAG CTTCGTAGAG 
TTTGTGGAGC GGTACCGTGT GCTGCTGCCA GGTGTGAAGC CGGCCTACAA GCAGGGCGAC 
CTCCGCGGGA CTTGCCAGCG CATGGCTGAG GCTGTGCTGG GCACCCACGA TGACTGGCAG 
ATAGGCAAAA CCAAGATCTT TCTGAAGGAC CACCATGACA TGCTGCTGGA AGTGGAGCGG 
GACAAAGCCA TCACCGACAG AGTCATCCTC CTTCAGAAAG TCATCCGGGG ATTCAAAGAC 
AGGTCTAACT TTCTGAAGCT GAAGAACGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGT 
CACAACTGTA GGAAGAACTA CGGGCTGATG CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG 
CACCGCTCCC GGAAGCTGCA CCAGCAGTAC CGCCTGGCCC GCCAGCGCAT CATCCAGTTC 
CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC AAGGCCTTCC GCCACCGCCT CTGGGCTGTG 
CTCACCGTGC AGGCCTATGC CCGGGGCATG ATCGCCCGCA GGCTGCACCA ACGCCTCAGG 
GCTGAGTATC TGTGGCGCCT CGAGGCTGAG AAAATGCGGC TGGCGGAGGA AGAGAAGCTT 
CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC 
CTGGCCCAGC TGGCTCGTGA GGACGCTGAG CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG 
CGGAAGAAGG AGCTCCTGGA GCAGATGGAA AGGGCCCGCC ATGAGCCTGT CAATCACTCA 
GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCAG GTGGCCTGCC AGGCCAGGAG 
GGCCAGGCAC CTAGTGGCTT TGAGGACCTG GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG 
GACCTGGATG CAGCCCTGCC CCTGCCTGAC GAGGATGAGG AGGACCTCTC TGAGTATAAA 
TTTGCCAAGT TCGCGGCCAC CTACTTCCAG GGGACAACCA CGCACTCCTA CACCCGGCGG 
CCACTCAAAC AGCCACTGCT CTACCATGAC GACGAGGGTG ACCAGCTGGC AGCCCTGGCG 
GTCTGGATCA CCATCCTCCG CTTCATGGGG GACCTCCCTG AGCCCAAGTA CCACACAGCC 
ATGAGTGATG GCAGTGAGAA GATCCCTGTG ATGACCAAGA TTTATGAGAC CCTGGGCAAG 
AAGACGTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG 
GGCCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCATT TGACTCTGAA AAAGAAGTCC 
AAGCTCACAG AGGAGGTGAC CAAGAGGCTG CATGACGGGG AGTCCACAGT GCAGGGCAAC 
AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATCGGCAAT 
GGCATCCTGC GGCCAGCACT CCGGTCAGTG CCGGGAGGCG GGGACACCAG GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO7A ( XM_011545044.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543346.1 Homo sapiens myosin VIIA (MYO7A), transcript variant X5, mRNA.

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CloneID	OHu59159
Clone ID Related Accession (Same CDS sequence)	XM_011545044.2
Accession Version	XM_011545044.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	unconventional myosin-VIIa isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545044.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGATTC TTCAGCAGGG GGACCATGTG TGGATGGACC TGAGATTGGG GCAGGAGTTC 
GACGTGCCCA TCGGGGCGGT GGTGAAGCTC TGCGACTCTG GGCAGGTCCA GGTGGTGGAT 
GATGAAGACA ATGAACACTG GATCTCTCCG CAGAACGCAA CGCACATCAA GCCTATGCAC 
CCCACGTCGG TCCACGGCGT GGAGGACATG ATCCGCCTGG GGGACCTCAA CGAGGCGGGC 
ATCTTGCGCA ACCTGCTTAT CCGCTACCGG GACCACCTCA TCTACACGTA TACGGGCTCC 
ATCCTGGTGG CTGTGAACCC CTACCAGCTG CTCTCCATCT ACTCGCCAGA GCACATCCGC 
CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAC 
TGCTACTTCA ACATGAAACG CAACAGCCGA GACCAGTGCT GCATCATCAG TGGGGAATCT 
GGGGCCGGGA AGACGGAGAG CACAAAGCTG ATCCTGCAGT TCCTGGCAGC CATCAGTGGG 
CAGCACTCGT GGATTGAGCA GCAGGTCTTG GAGGCCACCC CCATTCTGGA AGCATTTGGG 
AATGCCAAGA CCATCCGCAA TGACAACTCA AGCCGTTTCG GAAAGTACAT CGACATCCAC 
TTCAACAAGC GGGGCGCCAT CGAGGGCGCG AAGATTGAGC AGTACCTGCT GGAAAAGTCA 
CGTGTCTGTC GCCAGGCCCT GGATGAAAGG AACTACCACG TGTTCTACTG CATGCTGGAG 
GGTATGAGTG AGGATCAGAA GAAGAAGCTG GGCTTGGGCC AGGCCTCTGA CTACAACTAC 
TTGGCCATGG GTAACTGCAT AACCTGTGAG GGCCGGGTGG ACAGCCAGGA GTACGCCAAC 
ATCCGCTCCG CCATGAAGGT GCTCATGTTC ACTGACACCG AGAACTGGGA GATCTCGAAG 
CTCCTGGCTG CCATCCTGCA CCTGGGCAAC CTGCAGTATG AGGCACGCAC ATTTGAAAAC 
CTGGATGCCT GTGAGGTTCT CTTCTCCCCA TCGCTGGCCA CAGCTGCATC CCTGCTTGAG 
GTGAACCCCC CAGACCTGAT GAGCTGCCTG ACTAGCCGCA CCCTCATCAC CCGCGGGGAG 
ACGGTGTCCA CCCCACTGAG CAGGGAACAG GCACTGGACG TGCGCGACGC CTTCGTAAAG 
GGGATCTACG GGCGGCTGTT CGTGTGGATT GTGGACAAGA TCAACGCAGC AATTTACAAG 
CCTCCCTCCC AGGATGTGAA GAACTCTCGC AGGTCCATCG GCCTCCTGGA CATCTTTGGG 
TTTGAGAACT TTGCTGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTCGC CAATGAGCAC 
CTGCAGCAGT TCTTTGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAATA TGACCTGGAG 
AGCATTGACT GGCTGCACAT CGAGTTCACT GACAACCAGG ATGCCCTGGA CATGATTGCC 
AACAAGCCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACA 
GACACCACCA TGTTACACAA GCTGAACTCC CAGCACAAGC TCAACGCCAA CTACATCCCC 
CCCAAGAACA ACCATGAGAC CCAGTTTGGC ATCAACCATT TTGCAGGCAT CGTCTACTAT 
GAGACCCAAG GCTTCCTGGA GAAGAACCGA GACACCCTGC ATGGGGACAT TATCCAGCTG 
GTCCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT CGCCATGGGC 
GCCGAGACCA GGAAGCGCTC GCCCACACTT AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG 
CTGATGCGCA CGCTGGGTGC CTGCCAGCCC TTCTTTGTGC GATGCATCAA GCCCAATGAG 
TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGCGTGC GCCAGCTGCG GTACTCAGGA 
ATGATGGAGA CCATCCGAAT CCGCCGAGCT GGCTACCCCA TCCGCTACAG CTTCGTAGAG 
TTTGTGGAGC GGTACCGTGT GCTGCTGCCA GGTGTGAAGC CGGCCTACAA GCAGGGCGAC 
CTCCGCGGGA CTTGCCAGCG CATGGCTGAG GCTGTGCTGG GCACCCACGA TGACTGGCAG 
ATAGGCAAAA CCAAGATCTT TCTGAAGGAC CACCATGACA TGCTGCTGGA AGTGGAGCGG 
GACAAAGCCA TCACCGACAG AGTCATCCTC CTTCAGAAAG TCATCCGGGG ATTCAAAGAC 
AGGTCTAACT TTCTGAAGCT GAAGAACGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGT 
CACAACTGTA GGAAGAACTA CGGGCTGATG CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG 
CACCGCTCCC GGAAGCTGCA CCAGCAGTAC CGCCTGGCCC GCCAGCGCAT CATCCAGTTC 
CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC AAGGCCTTCC GCCACCGCCT CTGGGCTGTG 
CTCACCGTGC AGGCCTATGC CCGGGGCATG ATCGCCCGCA GGCTGCACCA ACGCCTCAGG 
GCTGAGTATC TGTGGCGCCT CGAGGCTGAG AAAATGCGGC TGGCGGAGGA AGAGAAGCTT 
CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC 
CTGGCCCAGC TGGCTCGTGA GGACGCTGAG CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG 
CGGAAGAAGG AGCTCCTGGA GCAGATGGAA AGGGCCCGCC ATGAGCCTGT CAATCACTCA 
GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCAG GTGGCCTGCC AGGCCAGGAG 
GGCCAGGCAC CTAGTGGCTT TGAGGACCTG GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG 
GACCTGGATG CAGCCCTGCC CCTGCCTGAC GAGGATGAGG AGGACCTCTC TGAGTATAAA 
TTTGCCAAGT TCGCGGCCAC CTACTTCCAG GGGACAACCA CGCACTCCTA CACCCGGCGG 
CCACTCAAAC AGCCACTGCT CTACCATGAC GACGAGGGTG ACCAGCTGGC AGCCCTGGCG 
GTCTGGATCA CCATCCTCCG CTTCATGGGG GACCTCCCTG AGCCCAAGTA CCACACAGCC 
ATGAGTGATG GCAGTGAGAA GATCCCTGTG ATGACCAAGA TTTATGAGAC CCTGGGCAAG 
AAGACGTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG 
GGCCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCATT TGACTCTGAA AAAGAAGTCC 
AAGCTCACAG AGGAGGTGAC CAAGAGGCTG CATGACGGGG AGTCCACAGT GCAGGGCAAC 
AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATCGGCAAT 
GGCATCCTGC GGCCAGCACT CCGGGACGAG ATCTACTGCC AGATCAGCAA GCAGCTGACC 
CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATTC TCGTGTCTCT CTGCGTGGGC 
TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG TACCTGCGGA ACTTCATCCA CGGGGGCCCG 
CCCGGCTACG CCCCGTACTG TGAGGAGCGC CTGAGAAGGA CCTTTGTCAA TGGGACACGG 
ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC AATCATGTTG 
CCCGTGACAT TCATGGATGG GACCACCAAG ACCCTGCTGA CGGACTCGGC AACCACGGCC 
AAGGAGCTCT GCAACGCGCT GGCCGACAAG ATCTCTCTCA AGGACCGGTT CGGGTTCTCC 
CTCTACATTG CCCTGTTTGA CAAGGTGTCC TCCCTGGGCA GCGGCAGTGA CCACGTCATG 
GACGCCATCT CCCAGTGCGA GCAGTACGCC AAGGAGCAGG GCGCCCAGGA GCGCAACGCC 
CCCTGGAGGC TCTTCTTCCG CAAAGAGGTC TTCACGCCCT GGCACAGCCC CTCCGAGGAC 
AACGTGGCCA CCAACCTCAT CTACCAGCAG GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC 
AGGTGTGAGA AGGAGGACGA CCTGGCTGAG CTGGCCTCCC AGCAGTACTT TGTAGACTAT 
GGCTCTGAGA TGATCCTGGA GCGCCTCCTG AACCTCGTGC CCACCTACAT CCCCGACCGC 
GAGATCACGC CCCTGAAGAC GCTGGAGAAG TGGGCCCAGC TGGCCATCGC CGCCCACAAG 
AAGGGGATTT ATGCCCAGAG GAGAACTGAT GCCCAGAAGG TCAAAGAGGA TGTGGTCAGT 
TATGCCCGCT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ATGAAGCCTA CAAATTCTCA 
GGCCCCAGTC TCCCCAAGAA CGACGTCATC GTGGCCGTCA ACTGGACGGG TGTGTACTTT 
GTGGATGAGC AGGAGCAGGT ACTTCTGGAG CTGTCCTTCC CAGAGATCAT GGCCGTGTCC 
AGCAGCAGGG AGTGCCGTGT CTGGCTCTCA CTGGGCTGCT CTGATCTTGG CTGTGCTGCG 
CCTCACTCAG GCTGGGCAGG ACTGACCCCG GCGGGGCCCT GTTCTCCGTG TTGGTCCTGC 
AGGGGAGCGA AAACGACGGC CCCCAGCTTC ACGCTGGCCA CCATCAAGGG GGACGAATAC 
ACCTTCACCT CCAGCAATGC TGAGGACATT CGTGACCTGG TGGTCACCTT CCTAGAGGGG 
CTCCGGAAGA GATCTAAGTA TGTTGTGGCC CTGCAGGATA ACCCCAACCC CGCAGGCGAG 
GAGTCAGGCT TCCTCAGCTT TGCCAAGGGA GACCTCATCA TCCTGGACCA TGACACGGGC 
GAGCAGGTCA TGAACTCGGG CTGGGCCAAC GGCATCAATG AGAGGACCAA GCAGCGTGGG 
GACTTCCCCA CCGACAGTGT GTACGTCATG CCCACTGTCA CCATGCCACC GCGGGAGATT 
GTGGCCCTGG TCACCATGAC TCCCGATCAG AGGCAGGACG TTGTCCGGCT CTTGCAGCTG 
CGAACGGCGG AGCCCGAGGT GCGTGCCAAG CCCTACACGC TGGAGGAGTT TTCCTATGAC 
TACTTCAGGC CCCCACCCAA GCACACGCTG AGCCGTGTCA TGGTGTCCAA GGCCCGAGGC 
AAGGACCGGC TGTGGAGCCA CACGCGGGAA CCGCTCAAGC AGGCGCTGCT CAAGAAGCTC 
CTGGGCAGTG AGGAGCTCTC GCAGGAGGCC TGCCTGGCCT TCATTGCTGT GCTCAAGTAC 
ATGGGCGACT ACCCGTCCAA GAGGACACGC TCCGTCAACG AGCTCACCGA CCAGATCTTT 
GAGGGTCCCC TGAAAGCCGA GCCCCTGAAG GACGAGGCAT ATGTGCAGAT CCTGAAGCAG 
CTGACCGACA ACCACATCAG GTACAGCGAG GAGCGGGGTT GGGAGCTGCT CTGGCTGTGC 
ACGGGCCTTT TCCCACCCAG CAACATCCTC CTGCCCCACG TGCAGCGCTT CCTGCAGTCC 
CGAAAGCACT GCCCACTCGC CATCGACTGC CTGCAACGGC TCCAGAAAGC CCTGAGAAAC 
GGGTCCCGGA AGTACCCTCC GCACCTGGTG GAGGTGGAGG CCATCCAGCA CAAGACCACC 
CAGATTTTCC ACAAAGTCTA CTTCCCTGAT GACACTGACG AGGCCTTCGA AGTGGAGTCC 
AGCACCAAGG CCAAGGACTT CTGCCAGAAC ATCGCCACCA GGCTGCTCCT CAAGTCCTCA 
GAGGGATTCA GCCTCTTTGT CAAAATTGCA GACAAGGTCC TCAGCGTTCC TGAGAATGAC 
TTCTTCTTTG ACTTTGTTCG ACACTTGACA GACTGGATAA AGAAAGCTCG GCCCATCAAG 
GACGGAATTG TGCCCTCACT CACCTACCAG GTGTTCTTCA TGAAGAAGCT GTGGACCACC 
ACGGTGCCAG GGAAGGATCC CATGGCCGAT TCCATCTTCC ACTATTACCA GGAGTTGCCC 
AAGTATCTCC GAGGCTACCA CAAGTGCACG CGGGAGGAGG TGCTGCAGCT GGGGGCGCTG 
ATCTACAGGG TCAAGTTCGA GGAGGACAAG TCCTACTTCC CCAGCATCCC CAAGCTGCTG 
CGGGAGCTGG TGCCCCAGGA CCTTATCCGG CAGGTCTCAC CTGATGACTG GAAGCGGTCC 
ATCGTCGCCT ACTTCAACAA GCACGCAGGG AAGTCCAAGG AGGAGGCCAA GCTGGCCTTC 
CTGAAGCTCA TCTTCAAGTG GCCCACCTTT GGCTCAGCCT TCTTCGAGGT GAAGGTACAC 
CATGGGCTTC TCAGAGCAGA GGAGGAGGGG AACAGGGCAT TGATAAAGCA CAGAGACTCC 
TCCCTAGGAC TGTGGGAAAG AGCCATGGCC TTGGACACCT GCCCTGGTGA GGGAAGGAGG 
GCAGCACCCT CAGCCTTGTC CCAGCTTAGC GTCTTAGCTG GGGTAAGTCC TGGGGACAGA 
GGGACCAGAA GAAGCTCAGC CTGGCAGTGG AGGCAGACAT GTAAACAGGC TGGGGTAATG 
GAGGATGGCC TGTGTTCTGA CACTGGGGAG CCCTCGCCCA GGTGGCAGAG CTCGGGAGGC 
TCTGCCGGGT GTGAGAAGGC CTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543346.1
CDS	345..7031
Translation	MVILQQGDHVWMDLRLGQEFDVPIGAVVKLCDSGQVQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNSRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQALDERNYHVFYCMLEGMSEDQKKKLGLGQASDYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHLGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVDKINAAIYKPPSQDVKNSRRSIGLLDIFGFENFAVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQDALDMIANKPMNIISLIDEESKFPKGTDTTMLHKLNSQHKLNANYIPPKNNHETQFGINHFAGIVYYETQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRRAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLKLKNAATLIQRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLHQQYRLARQRIIQFQARCRAYLVRKAFRHRLWAVLTVQAYARGMIARRLHQRLRAEYLWRLEAEKMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEAARRKKELLEQMERARHEPVNHSDMVDKMFGFLGTSGGLPGQEGQAPSGFEDLERGRREMVEEDLDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGEAQLPEGQKKSSVRHKLVHLTLKKKSKLTEEVTKRLHDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTAKELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHSPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLNLVPTYIPDREITPLKTLEKWAQLAIAAHKKGIYAQRRTDAQKVKEDVVSYARFKWPLLFSRFYEAYKFSGPSLPKNDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRECRVWLSLGCSDLGCAAPHSGWAGLTPAGPCSPCWSCRGAKTTAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDSVYVMPTVTMPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKLLGSEELSQEACLAFIAVLKYMGDYPSKRTRSVNELTDQIFEGPLKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIATRLLLKSSEGFSLFVKIADKVLSVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKVHHGLLRAEEEGNRALIKHRDSSLGLWERAMALDTCPGEGRRAAPSALSQLSVLAGVSPGDRGTRRSSAWQWRQTCKQAGVMEDGLCSDTGEPSPRWQSSGGSAGCEKAS

Target ORF information:

RefSeq Version	XM_011545044.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VIIA (MYO7A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545044.2

ORF Insert Sequence:

ATGGTGATTC TTCAGCAGGG GGACCATGTG TGGATGGACC TGAGATTGGG GCAGGAGTTC 
GACGTGCCCA TCGGGGCGGT GGTGAAGCTC TGCGACTCTG GGCAGGTCCA GGTGGTGGAT 
GATGAAGACA ATGAACACTG GATCTCTCCG CAGAACGCAA CGCACATCAA GCCTATGCAC 
CCCACGTCGG TCCACGGCGT GGAGGACATG ATCCGCCTGG GGGACCTCAA CGAGGCGGGC 
ATCTTGCGCA ACCTGCTTAT CCGCTACCGG GACCACCTCA TCTACACGTA TACGGGCTCC 
ATCCTGGTGG CTGTGAACCC CTACCAGCTG CTCTCCATCT ACTCGCCAGA GCACATCCGC 
CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAC 
TGCTACTTCA ACATGAAACG CAACAGCCGA GACCAGTGCT GCATCATCAG TGGGGAATCT 
GGGGCCGGGA AGACGGAGAG CACAAAGCTG ATCCTGCAGT TCCTGGCAGC CATCAGTGGG 
CAGCACTCGT GGATTGAGCA GCAGGTCTTG GAGGCCACCC CCATTCTGGA AGCATTTGGG 
AATGCCAAGA CCATCCGCAA TGACAACTCA AGCCGTTTCG GAAAGTACAT CGACATCCAC 
TTCAACAAGC GGGGCGCCAT CGAGGGCGCG AAGATTGAGC AGTACCTGCT GGAAAAGTCA 
CGTGTCTGTC GCCAGGCCCT GGATGAAAGG AACTACCACG TGTTCTACTG CATGCTGGAG 
GGTATGAGTG AGGATCAGAA GAAGAAGCTG GGCTTGGGCC AGGCCTCTGA CTACAACTAC 
TTGGCCATGG GTAACTGCAT AACCTGTGAG GGCCGGGTGG ACAGCCAGGA GTACGCCAAC 
ATCCGCTCCG CCATGAAGGT GCTCATGTTC ACTGACACCG AGAACTGGGA GATCTCGAAG 
CTCCTGGCTG CCATCCTGCA CCTGGGCAAC CTGCAGTATG AGGCACGCAC ATTTGAAAAC 
CTGGATGCCT GTGAGGTTCT CTTCTCCCCA TCGCTGGCCA CAGCTGCATC CCTGCTTGAG 
GTGAACCCCC CAGACCTGAT GAGCTGCCTG ACTAGCCGCA CCCTCATCAC CCGCGGGGAG 
ACGGTGTCCA CCCCACTGAG CAGGGAACAG GCACTGGACG TGCGCGACGC CTTCGTAAAG 
GGGATCTACG GGCGGCTGTT CGTGTGGATT GTGGACAAGA TCAACGCAGC AATTTACAAG 
CCTCCCTCCC AGGATGTGAA GAACTCTCGC AGGTCCATCG GCCTCCTGGA CATCTTTGGG 
TTTGAGAACT TTGCTGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTCGC CAATGAGCAC 
CTGCAGCAGT TCTTTGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAATA TGACCTGGAG 
AGCATTGACT GGCTGCACAT CGAGTTCACT GACAACCAGG ATGCCCTGGA CATGATTGCC 
AACAAGCCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACA 
GACACCACCA TGTTACACAA GCTGAACTCC CAGCACAAGC TCAACGCCAA CTACATCCCC 
CCCAAGAACA ACCATGAGAC CCAGTTTGGC ATCAACCATT TTGCAGGCAT CGTCTACTAT 
GAGACCCAAG GCTTCCTGGA GAAGAACCGA GACACCCTGC ATGGGGACAT TATCCAGCTG 
GTCCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT CGCCATGGGC 
GCCGAGACCA GGAAGCGCTC GCCCACACTT AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG 
CTGATGCGCA CGCTGGGTGC CTGCCAGCCC TTCTTTGTGC GATGCATCAA GCCCAATGAG 
TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGCGTGC GCCAGCTGCG GTACTCAGGA 
ATGATGGAGA CCATCCGAAT CCGCCGAGCT GGCTACCCCA TCCGCTACAG CTTCGTAGAG 
TTTGTGGAGC GGTACCGTGT GCTGCTGCCA GGTGTGAAGC CGGCCTACAA GCAGGGCGAC 
CTCCGCGGGA CTTGCCAGCG CATGGCTGAG GCTGTGCTGG GCACCCACGA TGACTGGCAG 
ATAGGCAAAA CCAAGATCTT TCTGAAGGAC CACCATGACA TGCTGCTGGA AGTGGAGCGG 
GACAAAGCCA TCACCGACAG AGTCATCCTC CTTCAGAAAG TCATCCGGGG ATTCAAAGAC 
AGGTCTAACT TTCTGAAGCT GAAGAACGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGT 
CACAACTGTA GGAAGAACTA CGGGCTGATG CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG 
CACCGCTCCC GGAAGCTGCA CCAGCAGTAC CGCCTGGCCC GCCAGCGCAT CATCCAGTTC 
CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC AAGGCCTTCC GCCACCGCCT CTGGGCTGTG 
CTCACCGTGC AGGCCTATGC CCGGGGCATG ATCGCCCGCA GGCTGCACCA ACGCCTCAGG 
GCTGAGTATC TGTGGCGCCT CGAGGCTGAG AAAATGCGGC TGGCGGAGGA AGAGAAGCTT 
CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC 
CTGGCCCAGC TGGCTCGTGA GGACGCTGAG CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG 
CGGAAGAAGG AGCTCCTGGA GCAGATGGAA AGGGCCCGCC ATGAGCCTGT CAATCACTCA 
GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCAG GTGGCCTGCC AGGCCAGGAG 
GGCCAGGCAC CTAGTGGCTT TGAGGACCTG GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG 
GACCTGGATG CAGCCCTGCC CCTGCCTGAC GAGGATGAGG AGGACCTCTC TGAGTATAAA 
TTTGCCAAGT TCGCGGCCAC CTACTTCCAG GGGACAACCA CGCACTCCTA CACCCGGCGG 
CCACTCAAAC AGCCACTGCT CTACCATGAC GACGAGGGTG ACCAGCTGGC AGCCCTGGCG 
GTCTGGATCA CCATCCTCCG CTTCATGGGG GACCTCCCTG AGCCCAAGTA CCACACAGCC 
ATGAGTGATG GCAGTGAGAA GATCCCTGTG ATGACCAAGA TTTATGAGAC CCTGGGCAAG 
AAGACGTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG 
GGCCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCATT TGACTCTGAA AAAGAAGTCC 
AAGCTCACAG AGGAGGTGAC CAAGAGGCTG CATGACGGGG AGTCCACAGT GCAGGGCAAC 
AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATCGGCAAT 
GGCATCCTGC GGCCAGCACT CCGGGACGAG ATCTACTGCC AGATCAGCAA GCAGCTGACC 
CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATTC TCGTGTCTCT CTGCGTGGGC 
TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG TACCTGCGGA ACTTCATCCA CGGGGGCCCG 
CCCGGCTACG CCCCGTACTG TGAGGAGCGC CTGAGAAGGA CCTTTGTCAA TGGGACACGG 
ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC AATCATGTTG 
CCCGTGACAT TCATGGATGG GACCACCAAG ACCCTGCTGA CGGACTCGGC AACCACGGCC 
AAGGAGCTCT GCAACGCGCT GGCCGACAAG ATCTCTCTCA AGGACCGGTT CGGGTTCTCC 
CTCTACATTG CCCTGTTTGA CAAGGTGTCC TCCCTGGGCA GCGGCAGTGA CCACGTCATG 
GACGCCATCT CCCAGTGCGA GCAGTACGCC AAGGAGCAGG GCGCCCAGGA GCGCAACGCC 
CCCTGGAGGC TCTTCTTCCG CAAAGAGGTC TTCACGCCCT GGCACAGCCC CTCCGAGGAC 
AACGTGGCCA CCAACCTCAT CTACCAGCAG GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC 
AGGTGTGAGA AGGAGGACGA CCTGGCTGAG CTGGCCTCCC AGCAGTACTT TGTAGACTAT 
GGCTCTGAGA TGATCCTGGA GCGCCTCCTG AACCTCGTGC CCACCTACAT CCCCGACCGC 
GAGATCACGC CCCTGAAGAC GCTGGAGAAG TGGGCCCAGC TGGCCATCGC CGCCCACAAG 
AAGGGGATTT ATGCCCAGAG GAGAACTGAT GCCCAGAAGG TCAAAGAGGA TGTGGTCAGT 
TATGCCCGCT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ATGAAGCCTA CAAATTCTCA 
GGCCCCAGTC TCCCCAAGAA CGACGTCATC GTGGCCGTCA ACTGGACGGG TGTGTACTTT 
GTGGATGAGC AGGAGCAGGT ACTTCTGGAG CTGTCCTTCC CAGAGATCAT GGCCGTGTCC 
AGCAGCAGGG AGTGCCGTGT CTGGCTCTCA CTGGGCTGCT CTGATCTTGG CTGTGCTGCG 
CCTCACTCAG GCTGGGCAGG ACTGACCCCG GCGGGGCCCT GTTCTCCGTG TTGGTCCTGC 
AGGGGAGCGA AAACGACGGC CCCCAGCTTC ACGCTGGCCA CCATCAAGGG GGACGAATAC 
ACCTTCACCT CCAGCAATGC TGAGGACATT CGTGACCTGG TGGTCACCTT CCTAGAGGGG 
CTCCGGAAGA GATCTAAGTA TGTTGTGGCC CTGCAGGATA ACCCCAACCC CGCAGGCGAG 
GAGTCAGGCT TCCTCAGCTT TGCCAAGGGA GACCTCATCA TCCTGGACCA TGACACGGGC 
GAGCAGGTCA TGAACTCGGG CTGGGCCAAC GGCATCAATG AGAGGACCAA GCAGCGTGGG 
GACTTCCCCA CCGACAGTGT GTACGTCATG CCCACTGTCA CCATGCCACC GCGGGAGATT 
GTGGCCCTGG TCACCATGAC TCCCGATCAG AGGCAGGACG TTGTCCGGCT CTTGCAGCTG 
CGAACGGCGG AGCCCGAGGT GCGTGCCAAG CCCTACACGC TGGAGGAGTT TTCCTATGAC 
TACTTCAGGC CCCCACCCAA GCACACGCTG AGCCGTGTCA TGGTGTCCAA GGCCCGAGGC 
AAGGACCGGC TGTGGAGCCA CACGCGGGAA CCGCTCAAGC AGGCGCTGCT CAAGAAGCTC 
CTGGGCAGTG AGGAGCTCTC GCAGGAGGCC TGCCTGGCCT TCATTGCTGT GCTCAAGTAC 
ATGGGCGACT ACCCGTCCAA GAGGACACGC TCCGTCAACG AGCTCACCGA CCAGATCTTT 
GAGGGTCCCC TGAAAGCCGA GCCCCTGAAG GACGAGGCAT ATGTGCAGAT CCTGAAGCAG 
CTGACCGACA ACCACATCAG GTACAGCGAG GAGCGGGGTT GGGAGCTGCT CTGGCTGTGC 
ACGGGCCTTT TCCCACCCAG CAACATCCTC CTGCCCCACG TGCAGCGCTT CCTGCAGTCC 
CGAAAGCACT GCCCACTCGC CATCGACTGC CTGCAACGGC TCCAGAAAGC CCTGAGAAAC 
GGGTCCCGGA AGTACCCTCC GCACCTGGTG GAGGTGGAGG CCATCCAGCA CAAGACCACC 
CAGATTTTCC ACAAAGTCTA CTTCCCTGAT GACACTGACG AGGCCTTCGA AGTGGAGTCC 
AGCACCAAGG CCAAGGACTT CTGCCAGAAC ATCGCCACCA GGCTGCTCCT CAAGTCCTCA 
GAGGGATTCA GCCTCTTTGT CAAAATTGCA GACAAGGTCC TCAGCGTTCC TGAGAATGAC 
TTCTTCTTTG ACTTTGTTCG ACACTTGACA GACTGGATAA AGAAAGCTCG GCCCATCAAG 
GACGGAATTG TGCCCTCACT CACCTACCAG GTGTTCTTCA TGAAGAAGCT GTGGACCACC 
ACGGTGCCAG GGAAGGATCC CATGGCCGAT TCCATCTTCC ACTATTACCA GGAGTTGCCC 
AAGTATCTCC GAGGCTACCA CAAGTGCACG CGGGAGGAGG TGCTGCAGCT GGGGGCGCTG 
ATCTACAGGG TCAAGTTCGA GGAGGACAAG TCCTACTTCC CCAGCATCCC CAAGCTGCTG 
CGGGAGCTGG TGCCCCAGGA CCTTATCCGG CAGGTCTCAC CTGATGACTG GAAGCGGTCC 
ATCGTCGCCT ACTTCAACAA GCACGCAGGG AAGTCCAAGG AGGAGGCCAA GCTGGCCTTC 
CTGAAGCTCA TCTTCAAGTG GCCCACCTTT GGCTCAGCCT TCTTCGAGGT GAAGGTACAC 
CATGGGCTTC TCAGAGCAGA GGAGGAGGGG AACAGGGCAT TGATAAAGCA CAGAGACTCC 
TCCCTAGGAC TGTGGGAAAG AGCCATGGCC TTGGACACCT GCCCTGGTGA GGGAAGGAGG 
GCAGCACCCT CAGCCTTGTC CCAGCTTAGC GTCTTAGCTG GGGTAAGTCC TGGGGACAGA 
GGGACCAGAA GAAGCTCAGC CTGGCAGTGG AGGCAGACAT GTAAACAGGC TGGGGTAATG 
GAGGATGGCC TGTGTTCTGA CACTGGGGAG CCCTCGCCCA GGTGGCAGAG CTCGGGAGGC 
TCTGCCGGGT GTGAGAAGGC CTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO7A ( XM_011545050.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543352.1 Homo sapiens myosin VIIA (MYO7A), transcript variant X12, mRNA.

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CloneID	OHu59165
Clone ID Related Accession (Same CDS sequence)	XM_011545050.2
Accession Version	XM_011545050.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	unconventional myosin-VIIa isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545050.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGGA ATTATCTGGA CGAAGGGACG TATACGGGCT CCATCCTGGT GGCTGTGAAC 
CCCTACCAGC TGCTCTCCAT CTACTCGCCA GAGCACATCC GCCAGTATAC CAACAAGAAG 
ATTGGGGAGA TGCCCCCCCA CATCTTTGCC ATTGCTGACA ACTGCTACTT CAACATGAAA 
CGCAACAGCC GAGACCAGTG CTGCATCATC AGTGGGGAAT CTGGGGCCGG GAAGACGGAG 
AGCACAAAGC TGATCCTGCA GTTCCTGGCA GCCATCAGTG GGCAGCACTC GTGGATTGAG 
CAGCAGGTCT TGGAGGCCAC CCCCATTCTG GAAGCATTTG GGAATGCCAA GACCATCCGC 
AATGACAACT CAAGCCGTTT CGGAAAGTAC ATCGACATCC ACTTCAACAA GCGGGGCGCC 
ATCGAGGGCG CGAAGATTGA GCAGTACCTG CTGGAAAAGT CACGTGTCTG TCGCCAGGCC 
CTGGATGAAA GGAACTACCA CGTGTTCTAC TGCATGCTGG AGGGTATGAG TGAGGATCAG 
AAGAAGAAGC TGGGCTTGGG CCAGGCCTCT GACTACAACT ACTTGGCCAT GGGTAACTGC 
ATAACCTGTG AGGGCCGGGT GGACAGCCAG GAGTACGCCA ACATCCGCTC CGCCATGAAG 
GTGCTCATGT TCACTGACAC CGAGAACTGG GAGATCTCGA AGCTCCTGGC TGCCATCCTG 
CACCTGGGCA ACCTGCAGTA TGAGGCACGC ACATTTGAAA ACCTGGATGC CTGTGAGGTT 
CTCTTCTCCC CATCGCTGGC CACAGCTGCA TCCCTGCTTG AGGTGAACCC CCCAGACCTG 
ATGAGCTGCC TGACTAGCCG CACCCTCATC ACCCGCGGGG AGACGGTGTC CACCCCACTG 
AGCAGGGAAC AGGCACTGGA CGTGCGCGAC GCCTTCGTAA AGGGGATCTA CGGGCGGCTG 
TTCGTGTGGA TTGTGGACAA GATCAACGCA GCAATTTACA AGCCTCCCTC CCAGGATGTG 
AAGAACTCTC GCAGGTCCAT CGGCCTCCTG GACATCTTTG GGTTTGAGAA CTTTGCTGTG 
AACAGCTTTG AGCAGCTCTG CATCAACTTC GCCAATGAGC ACCTGCAGCA GTTCTTTGTG 
CGGCACGTGT TCAAGCTGGA GCAGGAGGAA TATGACCTGG AGAGCATTGA CTGGCTGCAC 
ATCGAGTTCA CTGACAACCA GGATGCCCTG GACATGATTG CCAACAAGCC CATGAACATC 
ATCTCCCTCA TCGATGAGGA GAGCAAGTTC CCCAAGGGCA CAGACACCAC CATGTTACAC 
AAGCTGAACT CCCAGCACAA GCTCAACGCC AACTACATCC CCCCCAAGAA CAACCATGAG 
ACCCAGTTTG GCATCAACCA TTTTGCAGGC ATCGTCTACT ATGAGACCCA AGGCTTCCTG 
GAGAAGAACC GAGACACCCT GCATGGGGAC ATTATCCAGC TGGTCCACTC CTCCAGGAAC 
AAGTTCATCA AGCAGATCTT CCAGGCCGAT GTCGCCATGG GCGCCGAGAC CAGGAAGCGC 
TCGCCCACAC TTAGCAGCCA GTTCAAGCGG TCACTGGAGC TGCTGATGCG CACGCTGGGT 
GCCTGCCAGC CCTTCTTTGT GCGATGCATC AAGCCCAATG AGTTCAAGAA GCCCATGCTG 
TTCGACCGGC ACCTGTGCGT GCGCCAGCTG CGGTACTCAG GAATGATGGA GACCATCCGA 
ATCCGCCGAG CTGGCTACCC CATCCGCTAC AGCTTCGTAG AGTTTGTGGA GCGGTACCGT 
GTGCTGCTGC CAGGTGTGAA GCCGGCCTAC AAGCAGGGCG ACCTCCGCGG GACTTGCCAG 
CGCATGGCTG AGGCTGTGCT GGGCACCCAC GATGACTGGC AGATAGGCAA AACCAAGATC 
TTTCTGAAGG ACCACCATGA CATGCTGCTG GAAGTGGAGC GGGACAAAGC CATCACCGAC 
AGAGTCATCC TCCTTCAGAA AGTCATCCGG GGATTCAAAG ACAGGTCTAA CTTTCTGAAG 
CTGAAGAACG CTGCCACACT GATCCAGAGG CACTGGCGGG GTCACAACTG TAGGAAGAAC 
TACGGGCTGA TGCGTCTGGG CTTCCTGCGG CTGCAGGCCC TGCACCGCTC CCGGAAGCTG 
CACCAGCAGT ACCGCCTGGC CCGCCAGCGC ATCATCCAGT TCCAGGCCCG CTGCCGCGCC 
TATCTGGTGC GCAAGGCCTT CCGCCACCGC CTCTGGGCTG TGCTCACCGT GCAGGCCTAT 
GCCCGGGGCA TGATCGCCCG CAGGCTGCAC CAACGCCTCA GGGCTGAGTA TCTGTGGCGC 
CTCGAGGCTG AGAAAATGCG GCTGGCGGAG GAAGAGAAGC TTCGGAAGGA GATGAGCGCC 
AAGAAGGCCA AGGAGGAGGC CGAGCGCAAG CATCAGGAGC GCCTGGCCCA GCTGGCTCGT 
GAGGACGCTG AGCGGGAGCT GAAGGAGAAG GAGGCCGCTC GGCGGAAGAA GGAGCTCCTG 
GAGCAGATGG AAAGGGCCCG CCATGAGCCT GTCAATCACT CAGACATGGT GGACAAGATG 
TTTGGCTTCC TGGGGACTTC AGGTGGCCTG CCAGGCCAGG AGGGCCAGGC ACCTAGTGGC 
TTTGAGGACC TGGAGCGAGG GCGGAGGGAG ATGGTGGAGG AGGACCTGGA TGCAGCCCTG 
CCCCTGCCTG ACGAGGATGA GGAGGACCTC TCTGAGTATA AATTTGCCAA GTTCGCGGCC 
ACCTACTTCC AGGGGACAAC CACGCACTCC TACACCCGGC GGCCACTCAA ACAGCCACTG 
CTCTACCATG ACGACGAGGG TGACCAGCTG GCAGCCCTGG CGGTCTGGAT CACCATCCTC 
CGCTTCATGG GGGACCTCCC TGAGCCCAAG TACCACACAG CCATGAGTGA TGGCAGTGAG 
AAGATCCCTG TGATGACCAA GATTTATGAG ACCCTGGGCA AGAAGACGTA CAAGAGGGAG 
CTGCAGGCCC TGCAGGGCGA GGGCGAGGCC CAGCTCCCCG AGGGCCAGAA GAAGAGCAGT 
GTGAGGCACA AGCTGGTGCA TTTGACTCTG AAAAAGAAGT CCAAGCTCAC AGAGGAGGTG 
ACCAAGAGGC TGCATGACGG GGAGTCCACA GTGCAGGGCA ACAGCATGCT GGAGGACCGG 
CCCACCTCCA ACCTGGAGAA GCTGCACTTC ATCATCGGCA ATGGCATCCT GCGGCCAGCA 
CTCCGGGACG AGATCTACTG CCAGATCAGC AAGCAGCTGA CCCACAACCC CTCCAAGAGC 
AGCTATGCCC GGGGCTGGAT TCTCGTGTCT CTCTGCGTGG GCTGTTTCGC CCCCTCCGAG 
AAGTTTGTCA AGTACCTGCG GAACTTCATC CACGGGGGCC CGCCCGGCTA CGCCCCGTAC 
TGTGAGGAGC GCCTGAGAAG GACCTTTGTC AATGGGACAC GGACACAGCC GCCCAGCTGG 
CTGGAGCTGC AGGCCACCAA GTCCAAGAAG CCAATCATGT TGCCCGTGAC ATTCATGGAT 
GGGACCACCA AGACCCTGCT GACGGACTCG GCAACCACGG CCAAGGAGCT CTGCAACGCG 
CTGGCCGACA AGATCTCTCT CAAGGACCGG TTCGGGTTCT CCCTCTACAT TGCCCTGTTT 
GACAAGCAAC GTCATGCTCA GCAGAGGGGC CCCCGCCCAC AAGCCCAGGG GAGGTACCGT 
GTTGCTGGAC ACAGTTTGCC TGGATTTTTC TCACCGATGC TCTTATGTTT CCAGGTGTCC 
TCCCTGGGCA GCGGCAGTGA CCACGTCATG GACGCCATCT CCCAGTGCGA GCAGTACGCC 
AAGGAGCAGG GCGCCCAGGA GCGCAACGCC CCCTGGAGGC TCTTCTTCCG CAAAGAGGTC 
TTCACGCCCT GGCACAGCCC CTCCGAGGAC AACGTGGCCA CCAACCTCAT CTACCAGCAG 
GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC AGGTGTGAGA AGGAGGACGA CCTGGCTGAG 
CTGGCCTCCC AGCAGTACTT TGTAGACTAT GGCTCTGAGA TGATCCTGGA GCGCCTCCTG 
AACCTCGTGC CCACCTACAT CCCCGACCGC GAGATCACGC CCCTGAAGAC GCTGGAGAAG 
TGGGCCCAGC TGGCCATCGC CGCCCACAAG AAGGGGATTT ATGCCCAGAG GAGAACTGAT 
GCCCAGAAGG TCAAAGAGGA TGTGGTCAGT TATGCCCGCT TCAAGTGGCC CTTGCTCTTC 
TCCAGGTTTT ATGAAGCCTA CAAATTCTCA GGCCCCAGTC TCCCCAAGAA CGACGTCATC 
GTGGCCGTCA ACTGGACGGG TGTGTACTTT GTGGATGAGC AGGAGCAGGT ACTTCTGGAG 
CTGTCCTTCC CAGAGATCAT GGCCGTGTCC AGCAGCAGGG GAGCGAAAAC GACGGCCCCC 
AGCTTCACGC TGGCCACCAT CAAGGGGGAC GAATACACCT TCACCTCCAG CAATGCTGAG 
GACATTCGTG ACCTGGTGGT CACCTTCCTA GAGGGGCTCC GGAAGAGATC TAAGTATGTT 
GTGGCCCTGC AGGATAACCC CAACCCCGCA GGCGAGGAGT CAGGCTTCCT CAGCTTTGCC 
AAGGGAGACC TCATCATCCT GGACCATGAC ACGGGCGAGC AGGTCATGAA CTCGGGCTGG 
GCCAACGGCA TCAATGAGAG GACCAAGCAG CGTGGGGACT TCCCCACCGA CAGTGTGTAC 
GTCATGCCCA CTGTCACCAT GCCACCGCGG GAGATTGTGG CCCTGGTCAC CATGACTCCC 
GATCAGAGGC AGGACGTTGT CCGGCTCTTG CAGCTGCGAA CGGCGGAGCC CGAGGTGCGT 
GCCAAGCCCT ACACGCTGGA GGAGTTTTCC TATGACTACT TCAGGCCCCC ACCCAAGCAC 
ACGCTGAGCC GTGTCATGGT GTCCAAGGCC CGAGGCAAGG ACCGGCTGTG GAGCCACACG 
CGGGAACCGC TCAAGCAGGC GCTGCTCAAG AAGCTCCTGG GCAGTGAGGA GCTCTCGCAG 
GAGGCCTGCC TGGCCTTCAT TGCTGTGCTC AAGTACATGG GCGACTACCC GTCCAAGAGG 
ACACGCTCCG TCAACGAGCT CACCGACCAG ATCTTTGAGG GTCCCCTGAA AGCCGAGCCC 
CTGAAGGACG AGGCATATGT GCAGATCCTG AAGCAGCTGA CCGACAACCA CATCAGGTAC 
AGCGAGGAGC GGGGTTGGGA GCTGCTCTGG CTGTGCACGG GCCTTTTCCC ACCCAGCAAC 
ATCCTCCTGC CCCACGTGCA GCGCTTCCTG CAGTCCCGAA AGCACTGCCC ACTCGCCATC 
GACTGCCTGC AACGGCTCCA GAAAGCCCTG AGAAACGGGT CCCGGAAGTA CCCTCCGCAC 
CTGGTGGAGG TGGAGGCCAT CCAGCACAAG ACCACCCAGA TTTTCCACAA AGTCTACTTC 
CCTGATGACA CTGACGAGGC CTTCGAAGTG GAGTCCAGCA CCAAGGCCAA GGACTTCTGC 
CAGAACATCG CCACCAGGCT GCTCCTCAAG TCCTCAGAGG GATTCAGCCT CTTTGTCAAA 
ATTGCAGACA AGGTCCTCAG CGTTCCTGAG AATGACTTCT TCTTTGACTT TGTTCGACAC 
TTGACAGACT GGATAAAGAA AGCTCGGCCC ATCAAGGACG GAATTGTGCC CTCACTCACC 
TACCAGGTGT TCTTCATGAA GAAGCTGTGG ACCACCACGG TGCCAGGGAA GGATCCCATG 
GCCGATTCCA TCTTCCACTA TTACCAGGAG TTGCCCAAGT ATCTCCGAGG CTACCACAAG 
TGCACGCGGG AGGAGGTGCT GCAGCTGGGG GCGCTGATCT ACAGGGTCAA GTTCGAGGAG 
GACAAGTCCT ACTTCCCCAG CATCCCCAAG CTGCTGCGGG AGCTGGTGCC CCAGGACCTT 
ATCCGGCAGG TCTCACCTGA TGACTGGAAG CGGTCCATCG TCGCCTACTT CAACAAGCAC 
GCAGGGAAGT CCAAGGAGGA GGCCAAGCTG GCCTTCCTGA AGCTCATCTT CAAGTGGCCC 
ACCTTTGGCT CAGCCTTCTT CGAGGTGAAG GTACACCATG GGCTTCTCAG AGCAGAGGAG 
GAGGGGAACA GGGCATTGAT AAAGCACAGA GACTCCTCCC TAGGACTGTG GGAAAGAGCC 
ATGGCCTTGG ACACCTGCCC TGGTGAGGGA AGGAGGGCAG CACCCTCAGC CTTGTCCCAG 
CTTAGCGTCT TAGCTGGGGT AAGTCCTGGG GACAGAGGGA CCAGAAGAAG CTCAGCCTGG 
CAGTGGAGGC AGACATGTAA ACAGGCTGGG GTAATGGAGG ATGGCCTGTG TTCTGACACT 
GGGGAGCCCT CGCCCAGGTG GCAGAGCTCG GGAGGCTCTG CCGGGTGTGA GAAGGCCTCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543352.1
CDS	218..6640
Translation	MRRNYLDEGTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNSRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQALDERNYHVFYCMLEGMSEDQKKKLGLGQASDYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHLGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVDKINAAIYKPPSQDVKNSRRSIGLLDIFGFENFAVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQDALDMIANKPMNIISLIDEESKFPKGTDTTMLHKLNSQHKLNANYIPPKNNHETQFGINHFAGIVYYETQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRRAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLKLKNAATLIQRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLHQQYRLARQRIIQFQARCRAYLVRKAFRHRLWAVLTVQAYARGMIARRLHQRLRAEYLWRLEAEKMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEAARRKKELLEQMERARHEPVNHSDMVDKMFGFLGTSGGLPGQEGQAPSGFEDLERGRREMVEEDLDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGEAQLPEGQKKSSVRHKLVHLTLKKKSKLTEEVTKRLHDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTAKELCNALADKISLKDRFGFSLYIALFDKQRHAQQRGPRPQAQGRYRVAGHSLPGFFSPMLLCFQVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHSPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLNLVPTYIPDREITPLKTLEKWAQLAIAAHKKGIYAQRRTDAQKVKEDVVSYARFKWPLLFSRFYEAYKFSGPSLPKNDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRGAKTTAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDSVYVMPTVTMPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKLLGSEELSQEACLAFIAVLKYMGDYPSKRTRSVNELTDQIFEGPLKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIATRLLLKSSEGFSLFVKIADKVLSVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKVHHGLLRAEEEGNRALIKHRDSSLGLWERAMALDTCPGEGRRAAPSALSQLSVLAGVSPGDRGTRRSSAWQWRQTCKQAGVMEDGLCSDTGEPSPRWQSSGGSAGCEKAS

Target ORF information:

RefSeq Version	XM_011545050.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VIIA (MYO7A), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545050.2

ORF Insert Sequence:

ATGAGAAGGA ATTATCTGGA CGAAGGGACG TATACGGGCT CCATCCTGGT GGCTGTGAAC 
CCCTACCAGC TGCTCTCCAT CTACTCGCCA GAGCACATCC GCCAGTATAC CAACAAGAAG 
ATTGGGGAGA TGCCCCCCCA CATCTTTGCC ATTGCTGACA ACTGCTACTT CAACATGAAA 
CGCAACAGCC GAGACCAGTG CTGCATCATC AGTGGGGAAT CTGGGGCCGG GAAGACGGAG 
AGCACAAAGC TGATCCTGCA GTTCCTGGCA GCCATCAGTG GGCAGCACTC GTGGATTGAG 
CAGCAGGTCT TGGAGGCCAC CCCCATTCTG GAAGCATTTG GGAATGCCAA GACCATCCGC 
AATGACAACT CAAGCCGTTT CGGAAAGTAC ATCGACATCC ACTTCAACAA GCGGGGCGCC 
ATCGAGGGCG CGAAGATTGA GCAGTACCTG CTGGAAAAGT CACGTGTCTG TCGCCAGGCC 
CTGGATGAAA GGAACTACCA CGTGTTCTAC TGCATGCTGG AGGGTATGAG TGAGGATCAG 
AAGAAGAAGC TGGGCTTGGG CCAGGCCTCT GACTACAACT ACTTGGCCAT GGGTAACTGC 
ATAACCTGTG AGGGCCGGGT GGACAGCCAG GAGTACGCCA ACATCCGCTC CGCCATGAAG 
GTGCTCATGT TCACTGACAC CGAGAACTGG GAGATCTCGA AGCTCCTGGC TGCCATCCTG 
CACCTGGGCA ACCTGCAGTA TGAGGCACGC ACATTTGAAA ACCTGGATGC CTGTGAGGTT 
CTCTTCTCCC CATCGCTGGC CACAGCTGCA TCCCTGCTTG AGGTGAACCC CCCAGACCTG 
ATGAGCTGCC TGACTAGCCG CACCCTCATC ACCCGCGGGG AGACGGTGTC CACCCCACTG 
AGCAGGGAAC AGGCACTGGA CGTGCGCGAC GCCTTCGTAA AGGGGATCTA CGGGCGGCTG 
TTCGTGTGGA TTGTGGACAA GATCAACGCA GCAATTTACA AGCCTCCCTC CCAGGATGTG 
AAGAACTCTC GCAGGTCCAT CGGCCTCCTG GACATCTTTG GGTTTGAGAA CTTTGCTGTG 
AACAGCTTTG AGCAGCTCTG CATCAACTTC GCCAATGAGC ACCTGCAGCA GTTCTTTGTG 
CGGCACGTGT TCAAGCTGGA GCAGGAGGAA TATGACCTGG AGAGCATTGA CTGGCTGCAC 
ATCGAGTTCA CTGACAACCA GGATGCCCTG GACATGATTG CCAACAAGCC CATGAACATC 
ATCTCCCTCA TCGATGAGGA GAGCAAGTTC CCCAAGGGCA CAGACACCAC CATGTTACAC 
AAGCTGAACT CCCAGCACAA GCTCAACGCC AACTACATCC CCCCCAAGAA CAACCATGAG 
ACCCAGTTTG GCATCAACCA TTTTGCAGGC ATCGTCTACT ATGAGACCCA AGGCTTCCTG 
GAGAAGAACC GAGACACCCT GCATGGGGAC ATTATCCAGC TGGTCCACTC CTCCAGGAAC 
AAGTTCATCA AGCAGATCTT CCAGGCCGAT GTCGCCATGG GCGCCGAGAC CAGGAAGCGC 
TCGCCCACAC TTAGCAGCCA GTTCAAGCGG TCACTGGAGC TGCTGATGCG CACGCTGGGT 
GCCTGCCAGC CCTTCTTTGT GCGATGCATC AAGCCCAATG AGTTCAAGAA GCCCATGCTG 
TTCGACCGGC ACCTGTGCGT GCGCCAGCTG CGGTACTCAG GAATGATGGA GACCATCCGA 
ATCCGCCGAG CTGGCTACCC CATCCGCTAC AGCTTCGTAG AGTTTGTGGA GCGGTACCGT 
GTGCTGCTGC CAGGTGTGAA GCCGGCCTAC AAGCAGGGCG ACCTCCGCGG GACTTGCCAG 
CGCATGGCTG AGGCTGTGCT GGGCACCCAC GATGACTGGC AGATAGGCAA AACCAAGATC 
TTTCTGAAGG ACCACCATGA CATGCTGCTG GAAGTGGAGC GGGACAAAGC CATCACCGAC 
AGAGTCATCC TCCTTCAGAA AGTCATCCGG GGATTCAAAG ACAGGTCTAA CTTTCTGAAG 
CTGAAGAACG CTGCCACACT GATCCAGAGG CACTGGCGGG GTCACAACTG TAGGAAGAAC 
TACGGGCTGA TGCGTCTGGG CTTCCTGCGG CTGCAGGCCC TGCACCGCTC CCGGAAGCTG 
CACCAGCAGT ACCGCCTGGC CCGCCAGCGC ATCATCCAGT TCCAGGCCCG CTGCCGCGCC 
TATCTGGTGC GCAAGGCCTT CCGCCACCGC CTCTGGGCTG TGCTCACCGT GCAGGCCTAT 
GCCCGGGGCA TGATCGCCCG CAGGCTGCAC CAACGCCTCA GGGCTGAGTA TCTGTGGCGC 
CTCGAGGCTG AGAAAATGCG GCTGGCGGAG GAAGAGAAGC TTCGGAAGGA GATGAGCGCC 
AAGAAGGCCA AGGAGGAGGC CGAGCGCAAG CATCAGGAGC GCCTGGCCCA GCTGGCTCGT 
GAGGACGCTG AGCGGGAGCT GAAGGAGAAG GAGGCCGCTC GGCGGAAGAA GGAGCTCCTG 
GAGCAGATGG AAAGGGCCCG CCATGAGCCT GTCAATCACT CAGACATGGT GGACAAGATG 
TTTGGCTTCC TGGGGACTTC AGGTGGCCTG CCAGGCCAGG AGGGCCAGGC ACCTAGTGGC 
TTTGAGGACC TGGAGCGAGG GCGGAGGGAG ATGGTGGAGG AGGACCTGGA TGCAGCCCTG 
CCCCTGCCTG ACGAGGATGA GGAGGACCTC TCTGAGTATA AATTTGCCAA GTTCGCGGCC 
ACCTACTTCC AGGGGACAAC CACGCACTCC TACACCCGGC GGCCACTCAA ACAGCCACTG 
CTCTACCATG ACGACGAGGG TGACCAGCTG GCAGCCCTGG CGGTCTGGAT CACCATCCTC 
CGCTTCATGG GGGACCTCCC TGAGCCCAAG TACCACACAG CCATGAGTGA TGGCAGTGAG 
AAGATCCCTG TGATGACCAA GATTTATGAG ACCCTGGGCA AGAAGACGTA CAAGAGGGAG 
CTGCAGGCCC TGCAGGGCGA GGGCGAGGCC CAGCTCCCCG AGGGCCAGAA GAAGAGCAGT 
GTGAGGCACA AGCTGGTGCA TTTGACTCTG AAAAAGAAGT CCAAGCTCAC AGAGGAGGTG 
ACCAAGAGGC TGCATGACGG GGAGTCCACA GTGCAGGGCA ACAGCATGCT GGAGGACCGG 
CCCACCTCCA ACCTGGAGAA GCTGCACTTC ATCATCGGCA ATGGCATCCT GCGGCCAGCA 
CTCCGGGACG AGATCTACTG CCAGATCAGC AAGCAGCTGA CCCACAACCC CTCCAAGAGC 
AGCTATGCCC GGGGCTGGAT TCTCGTGTCT CTCTGCGTGG GCTGTTTCGC CCCCTCCGAG 
AAGTTTGTCA AGTACCTGCG GAACTTCATC CACGGGGGCC CGCCCGGCTA CGCCCCGTAC 
TGTGAGGAGC GCCTGAGAAG GACCTTTGTC AATGGGACAC GGACACAGCC GCCCAGCTGG 
CTGGAGCTGC AGGCCACCAA GTCCAAGAAG CCAATCATGT TGCCCGTGAC ATTCATGGAT 
GGGACCACCA AGACCCTGCT GACGGACTCG GCAACCACGG CCAAGGAGCT CTGCAACGCG 
CTGGCCGACA AGATCTCTCT CAAGGACCGG TTCGGGTTCT CCCTCTACAT TGCCCTGTTT 
GACAAGCAAC GTCATGCTCA GCAGAGGGGC CCCCGCCCAC AAGCCCAGGG GAGGTACCGT 
GTTGCTGGAC ACAGTTTGCC TGGATTTTTC TCACCGATGC TCTTATGTTT CCAGGTGTCC 
TCCCTGGGCA GCGGCAGTGA CCACGTCATG GACGCCATCT CCCAGTGCGA GCAGTACGCC 
AAGGAGCAGG GCGCCCAGGA GCGCAACGCC CCCTGGAGGC TCTTCTTCCG CAAAGAGGTC 
TTCACGCCCT GGCACAGCCC CTCCGAGGAC AACGTGGCCA CCAACCTCAT CTACCAGCAG 
GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC AGGTGTGAGA AGGAGGACGA CCTGGCTGAG 
CTGGCCTCCC AGCAGTACTT TGTAGACTAT GGCTCTGAGA TGATCCTGGA GCGCCTCCTG 
AACCTCGTGC CCACCTACAT CCCCGACCGC GAGATCACGC CCCTGAAGAC GCTGGAGAAG 
TGGGCCCAGC TGGCCATCGC CGCCCACAAG AAGGGGATTT ATGCCCAGAG GAGAACTGAT 
GCCCAGAAGG TCAAAGAGGA TGTGGTCAGT TATGCCCGCT TCAAGTGGCC CTTGCTCTTC 
TCCAGGTTTT ATGAAGCCTA CAAATTCTCA GGCCCCAGTC TCCCCAAGAA CGACGTCATC 
GTGGCCGTCA ACTGGACGGG TGTGTACTTT GTGGATGAGC AGGAGCAGGT ACTTCTGGAG 
CTGTCCTTCC CAGAGATCAT GGCCGTGTCC AGCAGCAGGG GAGCGAAAAC GACGGCCCCC 
AGCTTCACGC TGGCCACCAT CAAGGGGGAC GAATACACCT TCACCTCCAG CAATGCTGAG 
GACATTCGTG ACCTGGTGGT CACCTTCCTA GAGGGGCTCC GGAAGAGATC TAAGTATGTT 
GTGGCCCTGC AGGATAACCC CAACCCCGCA GGCGAGGAGT CAGGCTTCCT CAGCTTTGCC 
AAGGGAGACC TCATCATCCT GGACCATGAC ACGGGCGAGC AGGTCATGAA CTCGGGCTGG 
GCCAACGGCA TCAATGAGAG GACCAAGCAG CGTGGGGACT TCCCCACCGA CAGTGTGTAC 
GTCATGCCCA CTGTCACCAT GCCACCGCGG GAGATTGTGG CCCTGGTCAC CATGACTCCC 
GATCAGAGGC AGGACGTTGT CCGGCTCTTG CAGCTGCGAA CGGCGGAGCC CGAGGTGCGT 
GCCAAGCCCT ACACGCTGGA GGAGTTTTCC TATGACTACT TCAGGCCCCC ACCCAAGCAC 
ACGCTGAGCC GTGTCATGGT GTCCAAGGCC CGAGGCAAGG ACCGGCTGTG GAGCCACACG 
CGGGAACCGC TCAAGCAGGC GCTGCTCAAG AAGCTCCTGG GCAGTGAGGA GCTCTCGCAG 
GAGGCCTGCC TGGCCTTCAT TGCTGTGCTC AAGTACATGG GCGACTACCC GTCCAAGAGG 
ACACGCTCCG TCAACGAGCT CACCGACCAG ATCTTTGAGG GTCCCCTGAA AGCCGAGCCC 
CTGAAGGACG AGGCATATGT GCAGATCCTG AAGCAGCTGA CCGACAACCA CATCAGGTAC 
AGCGAGGAGC GGGGTTGGGA GCTGCTCTGG CTGTGCACGG GCCTTTTCCC ACCCAGCAAC 
ATCCTCCTGC CCCACGTGCA GCGCTTCCTG CAGTCCCGAA AGCACTGCCC ACTCGCCATC 
GACTGCCTGC AACGGCTCCA GAAAGCCCTG AGAAACGGGT CCCGGAAGTA CCCTCCGCAC 
CTGGTGGAGG TGGAGGCCAT CCAGCACAAG ACCACCCAGA TTTTCCACAA AGTCTACTTC 
CCTGATGACA CTGACGAGGC CTTCGAAGTG GAGTCCAGCA CCAAGGCCAA GGACTTCTGC 
CAGAACATCG CCACCAGGCT GCTCCTCAAG TCCTCAGAGG GATTCAGCCT CTTTGTCAAA 
ATTGCAGACA AGGTCCTCAG CGTTCCTGAG AATGACTTCT TCTTTGACTT TGTTCGACAC 
TTGACAGACT GGATAAAGAA AGCTCGGCCC ATCAAGGACG GAATTGTGCC CTCACTCACC 
TACCAGGTGT TCTTCATGAA GAAGCTGTGG ACCACCACGG TGCCAGGGAA GGATCCCATG 
GCCGATTCCA TCTTCCACTA TTACCAGGAG TTGCCCAAGT ATCTCCGAGG CTACCACAAG 
TGCACGCGGG AGGAGGTGCT GCAGCTGGGG GCGCTGATCT ACAGGGTCAA GTTCGAGGAG 
GACAAGTCCT ACTTCCCCAG CATCCCCAAG CTGCTGCGGG AGCTGGTGCC CCAGGACCTT 
ATCCGGCAGG TCTCACCTGA TGACTGGAAG CGGTCCATCG TCGCCTACTT CAACAAGCAC 
GCAGGGAAGT CCAAGGAGGA GGCCAAGCTG GCCTTCCTGA AGCTCATCTT CAAGTGGCCC 
ACCTTTGGCT CAGCCTTCTT CGAGGTGAAG GTACACCATG GGCTTCTCAG AGCAGAGGAG 
GAGGGGAACA GGGCATTGAT AAAGCACAGA GACTCCTCCC TAGGACTGTG GGAAAGAGCC 
ATGGCCTTGG ACACCTGCCC TGGTGAGGGA AGGAGGGCAG CACCCTCAGC CTTGTCCCAG 
CTTAGCGTCT TAGCTGGGGT AAGTCCTGGG GACAGAGGGA CCAGAAGAAG CTCAGCCTGG 
CAGTGGAGGC AGACATGTAA ACAGGCTGGG GTAATGGAGG ATGGCCTGTG TTCTGACACT 
GGGGAGCCCT CGCCCAGGTG GCAGAGCTCG GGAGGCTCTG CCGGGTGTGA GAAGGCCTCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO7A ( NM_001369365.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001356294.1 Homo sapiens myosin VIIA (MYO7A), transcript variant 4, mRNA.

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CloneID	OHu108137
Clone ID Related Accession (Same CDS sequence)	NM_001369365.1
Accession Version	NM_001369365.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	unconventional myosin-VIIa isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000752.4, KF511157.1 and AP001855.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC144519.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGACCTGA GATTGGGGCA GGAGTTCGAC GTGCCCATCG GGGCGGTGGT GAAGCTCTGC 
GACTCTGGGC AGGTCCAGGT GGTGGATGAT GAAGACAATG AACACTGGAT CTCTCCGCAG 
AACGCAACGC ACATCAAGCC TATGCACCCC ACGTCGGTCC ACGGCGTGGA GGACATGATC 
CGCCTGGGGG ACCTCAACGA GGCGGGCATC TTGCGCAACC TGCTTATCCG CTACCGGGAC 
CACCTCATCT ACACGTATAC GGGCTCCATC CTGGTGGCTG TGAACCCCTA CCAGCTGCTC 
TCCATCTACT CGCCAGAGCA CATCCGCCAG TATACCAACA AGAAGATTGG GGAGATGCCC 
CCCCACATCT TTGCCATTGC TGACAACTGC TACTTCAACA TGAAACGCAA CAGCCGAGAC 
CAGTGCTGCA TCATCAGTGG GGAATCTGGG GCCGGGAAGA CGGAGAGCAC AAAGCTGATC 
CTGCAGTTCC TGGCAGCCAT CAGTGGGCAG CACTCGTGGA TTGAGCAGCA GGTCTTGGAG 
GCCACCCCCA TTCTGGAAGC ATTTGGGAAT GCCAAGACCA TCCGCAATGA CAACTCAAGC 
CGTTTCGGAA AGTACATCGA CATCCACTTC AACAAGCGGG GCGCCATCGA GGGCGCGAAG 
ATTGAGCAGT ACCTGCTGGA AAAGTCACGT GTCTGTCGCC AGGCCCTGGA TGAAAGGAAC 
TACCACGTGT TCTACTGCAT GCTGGAGGGT ATGAGTGAGG ATCAGAAGAA GAAGCTGGGC 
TTGGGCCAGG CCTCTGACTA CAACTACTTG GCCATGGGTA ACTGCATAAC CTGTGAGGGC 
CGGGTGGACA GCCAGGAGTA CGCCAACATC CGCTCCGCCA TGAAGGTGCT CATGTTCACT 
GACACCGAGA ACTGGGAGAT CTCGAAGCTC CTGGCTGCCA TCCTGCACCT GGGCAACCTG 
CAGTATGAGG CACGCACATT TGAAAACCTG GATGCCTGTG AGGTTCTCTT CTCCCCATCG 
CTGGCCACAG CTGCATCCCT GCTTGAGGTG AACCCCCCAG ACCTGATGAG CTGCCTGACT 
AGCCGCACCC TCATCACCCG CGGGGAGACG GTGTCCACCC CACTGAGCAG GGAACAGGCA 
CTGGACGTGC GCGACGCCTT CGTAAAGGGG ATCTACGGGC GGCTGTTCGT GTGGATTGTG 
GACAAGATCA ACGCAGCAAT TTACAAGCCT CCCTCCCAGG ATGTGAAGAA CTCTCGCAGG 
TCCATCGGCC TCCTGGACAT CTTTGGGTTT GAGAACTTTG CTGTGAACAG CTTTGAGCAG 
CTCTGCATCA ACTTCGCCAA TGAGCACCTG CAGCAGTTCT TTGTGCGGCA CGTGTTCAAG 
CTGGAGCAGG AGGAATATGA CCTGGAGAGC ATTGACTGGC TGCACATCGA GTTCACTGAC 
AACCAGGATG CCCTGGACAT GATTGCCAAC AAGCCCATGA ACATCATCTC CCTCATCGAT 
GAGGAGAGCA AGTTCCCCAA GGGCACAGAC ACCACCATGT TACACAAGCT GAACTCCCAG 
CACAAGCTCA ACGCCAACTA CATCCCCCCC AAGAACAACC ATGAGACCCA GTTTGGCATC 
AACCATTTTG CAGGCATCGT CTACTATGAG ACCCAAGGCT TCCTGGAGAA GAACCGAGAC 
ACCCTGCATG GGGACATTAT CCAGCTGGTC CACTCCTCCA GGAACAAGTT CATCAAGCAG 
ATCTTCCAGG CCGATGTCGC CATGGGCGCC GAGACCAGGA AGCGCTCGCC CACACTTAGC 
AGCCAGTTCA AGCGGTCACT GGAGCTGCTG ATGCGCACGC TGGGTGCCTG CCAGCCCTTC 
TTTGTGCGAT GCATCAAGCC CAATGAGTTC AAGAAGCCCA TGCTGTTCGA CCGGCACCTG 
TGCGTGCGCC AGCTGCGGTA CTCAGGAATG ATGGAGACCA TCCGAATCCG CCGAGCTGGC 
TACCCCATCC GCTACAGCTT CGTAGAGTTT GTGGAGCGGT ACCGTGTGCT GCTGCCAGGT 
GTGAAGCCGG CCTACAAGCA GGGCGACCTC CGCGGGACTT GCCAGCGCAT GGCTGAGGCT 
GTGCTGGGCA CCCACGATGA CTGGCAGATA GGCAAAACCA AGATCTTTCT GAAGGACCAC 
CATGACATGC TGCTGGAAGT GGAGCGGGAC AAAGCCATCA CCGACAGAGT CATCCTCCTT 
CAGAAAGTCA TCCGGGGATT CAAAGACAGG TCTAACTTTC TGAAGCTGAA GAACGCTGCC 
ACACTGATCC AGAGGCACTG GCGGGGTCAC AACTGTAGGA AGAACTACGG GCTGATGCGT 
CTGGGCTTCC TGCGGCTGCA GGCCCTGCAC CGCTCCCGGA AGCTGCACCA GCAGTACCGC 
CTGGCCCGCC AGCGCATCAT CCAGTTCCAG GCCCGCTGCC GCGCCTATCT GGTGCGCAAG 
GCCTTCCGCC ACCGCCTCTG GGCTGTGCTC ACCGTGCAGG CCTATGCCCG GGGCATGATC 
GCCCGCAGGC TGCACCAACG CCTCAGGGCT GAGTATCTGT GGCGCCTCGA GGCTGAGAAA 
ATGCGGCTGG CGGAGGAAGA GAAGCTTCGG AAGGAGATGA GCGCCAAGAA GGCCAAGGAG 
GAGGCCGAGC GCAAGCATCA GGAGCGCCTG GCCCAGCTGG CTCGTGAGGA CGCTGAGCGG 
GAGCTGAAGG AGAAGGAGGC CGCTCGGCGG AAGAAGGAGC TCCTGGAGCA GATGGAAAGG 
GCCCGCCATG AGCCTGTCAA TCACTCAGAC ATGGTGGACA AGATGTTTGG CTTCCTGGGG 
ACTTCAGGTG GCCTGCCAGG CCAGGAGGGC CAGGCACCTA GTGGCTTTGA GGACCTGGAG 
CGAGGGCGGA GGGAGATGGT GGAGGAGGAC CTGGATGCAG CCCTGCCCCT GCCTGACGAG 
GATGAGGAGG ACCTCTCTGA GTATAAATTT GCCAAGTTCG CGGCCACCTA CTTCCAGGGG 
ACAACCACGC ACTCCTACAC CCGGCGGCCA CTCAAACAGC CACTGCTCTA CCATGACGAC 
GAGGGTGACC AGCTGGCAGC CCTGGCGGTC TGGATCACCA TCCTCCGCTT CATGGGGGAC 
CTCCCTGAGC CCAAGTACCA CACAGCCATG AGTGATGGCA GTGAGAAGAT CCCTGTGATG 
ACCAAGATTT ATGAGACCCT GGGCAAGAAG ACGTACAAGA GGGAGCTGCA GGCCCTGCAG 
GGCGAGGGCG AGGCCCAGCT CCCCGAGGGC CAGAAGAAGA GCAGTGTGAG GCACAAGCTG 
GTGCATTTGA CTCTGAAAAA GAAGTCCAAG CTCACAGAGG AGGTGACCAA GAGGCTGCAT 
GACGGGGAGT CCACAGTGCA GGGCAACAGC ATGCTGGAGG ACCGGCCCAC CTCCAACCTG 
GAGAAGCTGC ACTTCATCAT CGGCAATGGC ATCCTGCGGC CAGCACTCCG GGACGAGATC 
TACTGCCAGA TCAGCAAGCA GCTGACCCAC AACCCCTCCA AGAGCAGCTA TGCCCGGGGC 
TGGATTCTCG TGTCTCTCTG CGTGGGCTGT TTCGCCCCCT CCGAGAAGTT TGTCAAGTAC 
CTGCGGAACT TCATCCACGG GGGCCCGCCC GGCTACGCCC CGTACTGTGA GGAGCGCCTG 
AGAAGGACCT TTGTCAATGG GACACGGACA CAGCCGCCCA GCTGGCTGGA GCTGCAGGCC 
ACCAAGTCCA AGAAGCCAAT CATGTTGCCC GTGACATTCA TGGATGGGAC CACCAAGACC 
CTGCTGACGG ACTCGGCAAC CACGGCCAAG GAGCTCTGCA ACGCGCTGGC CGACAAGATC 
TCTCTCAAGG ACCGGTTCGG GTTCTCCCTC TACATTGCCC TGTTTGACAA GGTGTCCTCC 
CTGGGCAGCG GCAGTGACCA CGTCATGGAC GCCATCTCCC AGTGCGAGCA GTACGCCAAG 
GAGCAGGGCG CCCAGGAGCG CAACGCCCCC TGGAGGCTCT TCTTCCGCAA AGAGGTCTTC 
ACGCCCTGGC ACAGCCCCTC CGAGGACAAC GTGGCCACCA ACCTCATCTA CCAGCAGGTG 
GTGCGAGGAG TCAAGTTTGG GGAGTACAGG TGTGAGAAGG AGGACGACCT GGCTGAGCTG 
GCCTCCCAGC AGTACTTTGT AGACTATGGC TCTGAGATGA TCCTGGAGCG CCTCCTGAAC 
CTCGTGCCCA CCTACATCCC CGACCGCGAG ATCACGCCCC TGAAGACGCT GGAGAAGTGG 
GCCCAGCTGG CCATCGCCGC CCACAAGAAG GGGATTTATG CCCAGAGGAG AACTGATGCC 
CAGAAGGTCA AAGAGGATGT GGTCAGTTAT GCCCGCTTCA AGTGGCCCTT GCTCTTCTCC 
AGGTTTTATG AAGCCTACAA ATTCTCAGGC CCCAGTCTCC CCAAGAACGA CGTCATCGTG 
GCCGTCAACT GGACGGGTGT GTACTTTGTG GATGAGCAGG AGCAGGTACT TCTGGAGCTG 
TCCTTCCCAG AGATCATGGC CGTGTCCAGC AGCAGGGGAG CGAAAACGAC GGCCCCCAGC 
TTCACGCTGG CCACCATCAA GGGGGACGAA TACACCTTCA CCTCCAGCAA TGCTGAGGAC 
ATTCGTGACC TGGTGGTCAC CTTCCTAGAG GGGCTCCGGA AGAGATCTAA GTATGTTGTG 
GCCCTGCAGG ATAACCCCAA CCCCGCAGGC GAGGAGTCAG GCTTCCTCAG CTTTGCCAAG 
GGAGACCTCA TCATCCTGGA CCATGACACG GGCGAGCAGG TCATGAACTC GGGCTGGGCC 
AACGGCATCA ATGAGAGGAC CAAGCAGCGT GGGGACTTCC CCACCGACAG TGTGTACGTC 
ATGCCCACTG TCACCATGCC ACCGCGGGAG ATTGTGGCCC TGGTCACCAT GACTCCCGAT 
CAGAGGCAGG ACGTTGTCCG GCTCTTGCAG CTGCGAACGG CGGAGCCCGA GGTGCGTGCC 
AAGCCCTACA CGCTGGAGGA GTTTTCCTAT GACTACTTCA GGCCCCCACC CAAGCACACG 
CTGAGCCGTG TCATGGTGTC CAAGGCCCGA GGCAAGGACC GGCTGTGGAG CCACACGCGG 
GAACCGCTCA AGCAGGCGCT GCTCAAGAAG CTCCTGGGCA GTGAGGAGCT CTCGCAGGAG 
GCCTGCCTGG CCTTCATTGC TGTGCTCAAG TACATGGGCG ACTACCCGTC CAAGAGGACA 
CGCTCCGTCA ACGAGCTCAC CGACCAGATC TTTGAGGGTC CCCTGAAAGC CGAGCCCCTG 
AAGGACGAGG CATATGTGCA GATCCTGAAG CAGCTGACCG ACAACCACAT CAGGTACAGC 
GAGGAGCGGG GTTGGGAGCT GCTCTGGCTG TGCACGGGCC TTTTCCCACC CAGCAACATC 
CTCCTGCCCC ACGTGCAGCG CTTCCTGCAG TCCCGAAAGC ACTGCCCACT CGCCATCGAC 
TGCCTGCAAC GGCTCCAGAA AGCCCTGAGA AACGGGTCCC GGAAGTACCC TCCGCACCTG 
GTGGAGGTGG AGGCCATCCA GCACAAGACC ACCCAGATTT TCCACAAAGT CTACTTCCCT 
GATGACACTG ACGAGGCCTT CGAAGTGGAG TCCAGCACCA AGGCCAAGGA CTTCTGCCAG 
AACATCGCCA CCAGGCTGCT CCTCAAGTCC TCAGAGGGAT TCAGCCTCTT TGTCAAAATT 
GCAGACAAGG TCCTCAGCGT TCCTGAGAAT GACTTCTTCT TTGACTTTGT TCGACACTTG 
ACAGACTGGA TAAAGAAAGC TCGGCCCATC AAGGACGGAA TTGTGCCCTC ACTCACCTAC 
CAGGTGTTCT TCATGAAGAA GCTGTGGACC ACCACGGTGC CAGGGAAGGA TCCCATGGCC 
GATTCCATCT TCCACTATTA CCAGGAGTTG CCCAAGTATC TCCGAGGCTA CCACAAGTGC 
ACGCGGGAGG AGGTGCTGCA GCTGGGGGCG CTGATCTACA GGGTCAAGTT CGAGGAGGAC 
AAGTCCTACT TCCCCAGCAT CCCCAAGCTG CTGCGGGAGC TGGTGCCCCA GGACCTTATC 
CGGCAGGTCT CACCTGATGA CTGGAAGCGG TCCATCGTCG CCTACTTCAA CAAGCACGCA 
GGGAAGTCCA AGGAGGAGGC CAAGCTGGCC TTCCTGAAGC TCATCTTCAA GTGGCCCACC 
TTTGGCTCAG CCTTCTTCGA GGTGAAGCAA ACTACGGAGC CAAACTTCCC TGAGATCCTC 
CTAATTGCCA TCAACAAGTA TGGGGTCAGC CTCATCGATC CCAAAACGAA GGATATCCTC 
ACCACTCATC CCTTCACCAA GATCTCCAAC TGGAGCAGCG GCAACACCTA CTTCCACATC 
ACCATTGGGA ACTTGGTGCG CGGGAGCAAA CTGCTCTGCG AGACGTCACT GGGCTACAAG 
ATGGATGACC TCCTGACTTC CTACATTAGC CAGATGCTCA CAGCCATGAG CAAACAGCGG 
GGCTCCAGGA GCGGCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356294.1
CDS	404..6904
Translation	MDLRLGQEFDVPIGAVVKLCDSGQVQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNSRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQALDERNYHVFYCMLEGMSEDQKKKLGLGQASDYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHLGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVDKINAAIYKPPSQDVKNSRRSIGLLDIFGFENFAVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQDALDMIANKPMNIISLIDEESKFPKGTDTTMLHKLNSQHKLNANYIPPKNNHETQFGINHFAGIVYYETQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRRAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLKLKNAATLIQRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLHQQYRLARQRIIQFQARCRAYLVRKAFRHRLWAVLTVQAYARGMIARRLHQRLRAEYLWRLEAEKMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEAARRKKELLEQMERARHEPVNHSDMVDKMFGFLGTSGGLPGQEGQAPSGFEDLERGRREMVEEDLDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGEAQLPEGQKKSSVRHKLVHLTLKKKSKLTEEVTKRLHDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTAKELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHSPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLNLVPTYIPDREITPLKTLEKWAQLAIAAHKKGIYAQRRTDAQKVKEDVVSYARFKWPLLFSRFYEAYKFSGPSLPKNDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRGAKTTAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDSVYVMPTVTMPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKLLGSEELSQEACLAFIAVLKYMGDYPSKRTRSVNELTDQIFEGPLKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIATRLLLKSSEGFSLFVKIADKVLSVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKQTTEPNFPEILLIAINKYGVSLIDPKTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRGSRSGK

Target ORF information:

RefSeq Version	NM_001369365.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VIIA (MYO7A), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369365.1

ORF Insert Sequence:

ATGGACCTGA GATTGGGGCA GGAGTTCGAC GTGCCCATCG GGGCGGTGGT GAAGCTCTGC 
GACTCTGGGC AGGTCCAGGT GGTGGATGAT GAAGACAATG AACACTGGAT CTCTCCGCAG 
AACGCAACGC ACATCAAGCC TATGCACCCC ACGTCGGTCC ACGGCGTGGA GGACATGATC 
CGCCTGGGGG ACCTCAACGA GGCGGGCATC TTGCGCAACC TGCTTATCCG CTACCGGGAC 
CACCTCATCT ACACGTATAC GGGCTCCATC CTGGTGGCTG TGAACCCCTA CCAGCTGCTC 
TCCATCTACT CGCCAGAGCA CATCCGCCAG TATACCAACA AGAAGATTGG GGAGATGCCC 
CCCCACATCT TTGCCATTGC TGACAACTGC TACTTCAACA TGAAACGCAA CAGCCGAGAC 
CAGTGCTGCA TCATCAGTGG GGAATCTGGG GCCGGGAAGA CGGAGAGCAC AAAGCTGATC 
CTGCAGTTCC TGGCAGCCAT CAGTGGGCAG CACTCGTGGA TTGAGCAGCA GGTCTTGGAG 
GCCACCCCCA TTCTGGAAGC ATTTGGGAAT GCCAAGACCA TCCGCAATGA CAACTCAAGC 
CGTTTCGGAA AGTACATCGA CATCCACTTC AACAAGCGGG GCGCCATCGA GGGCGCGAAG 
ATTGAGCAGT ACCTGCTGGA AAAGTCACGT GTCTGTCGCC AGGCCCTGGA TGAAAGGAAC 
TACCACGTGT TCTACTGCAT GCTGGAGGGT ATGAGTGAGG ATCAGAAGAA GAAGCTGGGC 
TTGGGCCAGG CCTCTGACTA CAACTACTTG GCCATGGGTA ACTGCATAAC CTGTGAGGGC 
CGGGTGGACA GCCAGGAGTA CGCCAACATC CGCTCCGCCA TGAAGGTGCT CATGTTCACT 
GACACCGAGA ACTGGGAGAT CTCGAAGCTC CTGGCTGCCA TCCTGCACCT GGGCAACCTG 
CAGTATGAGG CACGCACATT TGAAAACCTG GATGCCTGTG AGGTTCTCTT CTCCCCATCG 
CTGGCCACAG CTGCATCCCT GCTTGAGGTG AACCCCCCAG ACCTGATGAG CTGCCTGACT 
AGCCGCACCC TCATCACCCG CGGGGAGACG GTGTCCACCC CACTGAGCAG GGAACAGGCA 
CTGGACGTGC GCGACGCCTT CGTAAAGGGG ATCTACGGGC GGCTGTTCGT GTGGATTGTG 
GACAAGATCA ACGCAGCAAT TTACAAGCCT CCCTCCCAGG ATGTGAAGAA CTCTCGCAGG 
TCCATCGGCC TCCTGGACAT CTTTGGGTTT GAGAACTTTG CTGTGAACAG CTTTGAGCAG 
CTCTGCATCA ACTTCGCCAA TGAGCACCTG CAGCAGTTCT TTGTGCGGCA CGTGTTCAAG 
CTGGAGCAGG AGGAATATGA CCTGGAGAGC ATTGACTGGC TGCACATCGA GTTCACTGAC 
AACCAGGATG CCCTGGACAT GATTGCCAAC AAGCCCATGA ACATCATCTC CCTCATCGAT 
GAGGAGAGCA AGTTCCCCAA GGGCACAGAC ACCACCATGT TACACAAGCT GAACTCCCAG 
CACAAGCTCA ACGCCAACTA CATCCCCCCC AAGAACAACC ATGAGACCCA GTTTGGCATC 
AACCATTTTG CAGGCATCGT CTACTATGAG ACCCAAGGCT TCCTGGAGAA GAACCGAGAC 
ACCCTGCATG GGGACATTAT CCAGCTGGTC CACTCCTCCA GGAACAAGTT CATCAAGCAG 
ATCTTCCAGG CCGATGTCGC CATGGGCGCC GAGACCAGGA AGCGCTCGCC CACACTTAGC 
AGCCAGTTCA AGCGGTCACT GGAGCTGCTG ATGCGCACGC TGGGTGCCTG CCAGCCCTTC 
TTTGTGCGAT GCATCAAGCC CAATGAGTTC AAGAAGCCCA TGCTGTTCGA CCGGCACCTG 
TGCGTGCGCC AGCTGCGGTA CTCAGGAATG ATGGAGACCA TCCGAATCCG CCGAGCTGGC 
TACCCCATCC GCTACAGCTT CGTAGAGTTT GTGGAGCGGT ACCGTGTGCT GCTGCCAGGT 
GTGAAGCCGG CCTACAAGCA GGGCGACCTC CGCGGGACTT GCCAGCGCAT GGCTGAGGCT 
GTGCTGGGCA CCCACGATGA CTGGCAGATA GGCAAAACCA AGATCTTTCT GAAGGACCAC 
CATGACATGC TGCTGGAAGT GGAGCGGGAC AAAGCCATCA CCGACAGAGT CATCCTCCTT 
CAGAAAGTCA TCCGGGGATT CAAAGACAGG TCTAACTTTC TGAAGCTGAA GAACGCTGCC 
ACACTGATCC AGAGGCACTG GCGGGGTCAC AACTGTAGGA AGAACTACGG GCTGATGCGT 
CTGGGCTTCC TGCGGCTGCA GGCCCTGCAC CGCTCCCGGA AGCTGCACCA GCAGTACCGC 
CTGGCCCGCC AGCGCATCAT CCAGTTCCAG GCCCGCTGCC GCGCCTATCT GGTGCGCAAG 
GCCTTCCGCC ACCGCCTCTG GGCTGTGCTC ACCGTGCAGG CCTATGCCCG GGGCATGATC 
GCCCGCAGGC TGCACCAACG CCTCAGGGCT GAGTATCTGT GGCGCCTCGA GGCTGAGAAA 
ATGCGGCTGG CGGAGGAAGA GAAGCTTCGG AAGGAGATGA GCGCCAAGAA GGCCAAGGAG 
GAGGCCGAGC GCAAGCATCA GGAGCGCCTG GCCCAGCTGG CTCGTGAGGA CGCTGAGCGG 
GAGCTGAAGG AGAAGGAGGC CGCTCGGCGG AAGAAGGAGC TCCTGGAGCA GATGGAAAGG 
GCCCGCCATG AGCCTGTCAA TCACTCAGAC ATGGTGGACA AGATGTTTGG CTTCCTGGGG 
ACTTCAGGTG GCCTGCCAGG CCAGGAGGGC CAGGCACCTA GTGGCTTTGA GGACCTGGAG 
CGAGGGCGGA GGGAGATGGT GGAGGAGGAC CTGGATGCAG CCCTGCCCCT GCCTGACGAG 
GATGAGGAGG ACCTCTCTGA GTATAAATTT GCCAAGTTCG CGGCCACCTA CTTCCAGGGG 
ACAACCACGC ACTCCTACAC CCGGCGGCCA CTCAAACAGC CACTGCTCTA CCATGACGAC 
GAGGGTGACC AGCTGGCAGC CCTGGCGGTC TGGATCACCA TCCTCCGCTT CATGGGGGAC 
CTCCCTGAGC CCAAGTACCA CACAGCCATG AGTGATGGCA GTGAGAAGAT CCCTGTGATG 
ACCAAGATTT ATGAGACCCT GGGCAAGAAG ACGTACAAGA GGGAGCTGCA GGCCCTGCAG 
GGCGAGGGCG AGGCCCAGCT CCCCGAGGGC CAGAAGAAGA GCAGTGTGAG GCACAAGCTG 
GTGCATTTGA CTCTGAAAAA GAAGTCCAAG CTCACAGAGG AGGTGACCAA GAGGCTGCAT 
GACGGGGAGT CCACAGTGCA GGGCAACAGC ATGCTGGAGG ACCGGCCCAC CTCCAACCTG 
GAGAAGCTGC ACTTCATCAT CGGCAATGGC ATCCTGCGGC CAGCACTCCG GGACGAGATC 
TACTGCCAGA TCAGCAAGCA GCTGACCCAC AACCCCTCCA AGAGCAGCTA TGCCCGGGGC 
TGGATTCTCG TGTCTCTCTG CGTGGGCTGT TTCGCCCCCT CCGAGAAGTT TGTCAAGTAC 
CTGCGGAACT TCATCCACGG GGGCCCGCCC GGCTACGCCC CGTACTGTGA GGAGCGCCTG 
AGAAGGACCT TTGTCAATGG GACACGGACA CAGCCGCCCA GCTGGCTGGA GCTGCAGGCC 
ACCAAGTCCA AGAAGCCAAT CATGTTGCCC GTGACATTCA TGGATGGGAC CACCAAGACC 
CTGCTGACGG ACTCGGCAAC CACGGCCAAG GAGCTCTGCA ACGCGCTGGC CGACAAGATC 
TCTCTCAAGG ACCGGTTCGG GTTCTCCCTC TACATTGCCC TGTTTGACAA GGTGTCCTCC 
CTGGGCAGCG GCAGTGACCA CGTCATGGAC GCCATCTCCC AGTGCGAGCA GTACGCCAAG 
GAGCAGGGCG CCCAGGAGCG CAACGCCCCC TGGAGGCTCT TCTTCCGCAA AGAGGTCTTC 
ACGCCCTGGC ACAGCCCCTC CGAGGACAAC GTGGCCACCA ACCTCATCTA CCAGCAGGTG 
GTGCGAGGAG TCAAGTTTGG GGAGTACAGG TGTGAGAAGG AGGACGACCT GGCTGAGCTG 
GCCTCCCAGC AGTACTTTGT AGACTATGGC TCTGAGATGA TCCTGGAGCG CCTCCTGAAC 
CTCGTGCCCA CCTACATCCC CGACCGCGAG ATCACGCCCC TGAAGACGCT GGAGAAGTGG 
GCCCAGCTGG CCATCGCCGC CCACAAGAAG GGGATTTATG CCCAGAGGAG AACTGATGCC 
CAGAAGGTCA AAGAGGATGT GGTCAGTTAT GCCCGCTTCA AGTGGCCCTT GCTCTTCTCC 
AGGTTTTATG AAGCCTACAA ATTCTCAGGC CCCAGTCTCC CCAAGAACGA CGTCATCGTG 
GCCGTCAACT GGACGGGTGT GTACTTTGTG GATGAGCAGG AGCAGGTACT TCTGGAGCTG 
TCCTTCCCAG AGATCATGGC CGTGTCCAGC AGCAGGGGAG CGAAAACGAC GGCCCCCAGC 
TTCACGCTGG CCACCATCAA GGGGGACGAA TACACCTTCA CCTCCAGCAA TGCTGAGGAC 
ATTCGTGACC TGGTGGTCAC CTTCCTAGAG GGGCTCCGGA AGAGATCTAA GTATGTTGTG 
GCCCTGCAGG ATAACCCCAA CCCCGCAGGC GAGGAGTCAG GCTTCCTCAG CTTTGCCAAG 
GGAGACCTCA TCATCCTGGA CCATGACACG GGCGAGCAGG TCATGAACTC GGGCTGGGCC 
AACGGCATCA ATGAGAGGAC CAAGCAGCGT GGGGACTTCC CCACCGACAG TGTGTACGTC 
ATGCCCACTG TCACCATGCC ACCGCGGGAG ATTGTGGCCC TGGTCACCAT GACTCCCGAT 
CAGAGGCAGG ACGTTGTCCG GCTCTTGCAG CTGCGAACGG CGGAGCCCGA GGTGCGTGCC 
AAGCCCTACA CGCTGGAGGA GTTTTCCTAT GACTACTTCA GGCCCCCACC CAAGCACACG 
CTGAGCCGTG TCATGGTGTC CAAGGCCCGA GGCAAGGACC GGCTGTGGAG CCACACGCGG 
GAACCGCTCA AGCAGGCGCT GCTCAAGAAG CTCCTGGGCA GTGAGGAGCT CTCGCAGGAG 
GCCTGCCTGG CCTTCATTGC TGTGCTCAAG TACATGGGCG ACTACCCGTC CAAGAGGACA 
CGCTCCGTCA ACGAGCTCAC CGACCAGATC TTTGAGGGTC CCCTGAAAGC CGAGCCCCTG 
AAGGACGAGG CATATGTGCA GATCCTGAAG CAGCTGACCG ACAACCACAT CAGGTACAGC 
GAGGAGCGGG GTTGGGAGCT GCTCTGGCTG TGCACGGGCC TTTTCCCACC CAGCAACATC 
CTCCTGCCCC ACGTGCAGCG CTTCCTGCAG TCCCGAAAGC ACTGCCCACT CGCCATCGAC 
TGCCTGCAAC GGCTCCAGAA AGCCCTGAGA AACGGGTCCC GGAAGTACCC TCCGCACCTG 
GTGGAGGTGG AGGCCATCCA GCACAAGACC ACCCAGATTT TCCACAAAGT CTACTTCCCT 
GATGACACTG ACGAGGCCTT CGAAGTGGAG TCCAGCACCA AGGCCAAGGA CTTCTGCCAG 
AACATCGCCA CCAGGCTGCT CCTCAAGTCC TCAGAGGGAT TCAGCCTCTT TGTCAAAATT 
GCAGACAAGG TCCTCAGCGT TCCTGAGAAT GACTTCTTCT TTGACTTTGT TCGACACTTG 
ACAGACTGGA TAAAGAAAGC TCGGCCCATC AAGGACGGAA TTGTGCCCTC ACTCACCTAC 
CAGGTGTTCT TCATGAAGAA GCTGTGGACC ACCACGGTGC CAGGGAAGGA TCCCATGGCC 
GATTCCATCT TCCACTATTA CCAGGAGTTG CCCAAGTATC TCCGAGGCTA CCACAAGTGC 
ACGCGGGAGG AGGTGCTGCA GCTGGGGGCG CTGATCTACA GGGTCAAGTT CGAGGAGGAC 
AAGTCCTACT TCCCCAGCAT CCCCAAGCTG CTGCGGGAGC TGGTGCCCCA GGACCTTATC 
CGGCAGGTCT CACCTGATGA CTGGAAGCGG TCCATCGTCG CCTACTTCAA CAAGCACGCA 
GGGAAGTCCA AGGAGGAGGC CAAGCTGGCC TTCCTGAAGC TCATCTTCAA GTGGCCCACC 
TTTGGCTCAG CCTTCTTCGA GGTGAAGCAA ACTACGGAGC CAAACTTCCC TGAGATCCTC 
CTAATTGCCA TCAACAAGTA TGGGGTCAGC CTCATCGATC CCAAAACGAA GGATATCCTC 
ACCACTCATC CCTTCACCAA GATCTCCAAC TGGAGCAGCG GCAACACCTA CTTCCACATC 
ACCATTGGGA ACTTGGTGCG CGGGAGCAAA CTGCTCTGCG AGACGTCACT GGGCTACAAG 
ATGGATGACC TCCTGACTTC CTACATTAGC CAGATGCTCA CAGCCATGAG CAAACAGCGG 
GGCTCCAGGA GCGGCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO7A ( XM_011545046.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543348.2 Homo sapiens myosin VIIA (MYO7A), transcript variant X1, mRNA.

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CloneID	OHu92254
Clone ID Related Accession (Same CDS sequence)	XM_011545046.2
Accession Version	XM_011545046.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	unconventional myosin-VIIa isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545046.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACC CCGCCGACCC TGCCGACCCC GCGCGCATCC CGCAGCCCGG CAAGTGGGCG 
CTGGTGCGCA GCCTGAACCA GGCGTTCAAG ACCTACGCCG TCCTGCCGGG GGACCATGTG 
TGGATGGACC TGAGATTGGG GCAGGAGTTC GACGTGCCCA TCGGGGCGGT GGTGAAGCTC 
TGCGACTCTG GGCAGGTCCA GGTGGTGGAT GATGAAGACA ATGAACACTG GATCTCTCCG 
CAGAACGCAA CGCACATCAA GCCTATGCAC CCCACGTCGG TCCACGGCGT GGAGGACATG 
ATCCGCCTGG GGGACCTCAA CGAGGCGGGC ATCTTGCGCA ACCTGCTTAT CCGCTACCGG 
GACCACCTCA TCTACACGTA TACGGGCTCC ATCCTGGTGG CTGTGAACCC CTACCAGCTG 
CTCTCCATCT ACTCGCCAGA GCACATCCGC CAGTATACCA ACAAGAAGAT TGGGGAGATG 
CCCCCCCACA TCTTTGCCAT TGCTGACAAC TGCTACTTCA ACATGAAACG CAACAGCCGA 
GACCAGTGCT GCATCATCAG TGGGGAATCT GGGGCCGGGA AGACGGAGAG CACAAAGCTG 
ATCCTGCAGT TCCTGGCAGC CATCAGTGGG CAGCACTCGT GGATTGAGCA GCAGGTCTTG 
GAGGCCACCC CCATTCTGGA AGCATTTGGG AATGCCAAGA CCATCCGCAA TGACAACTCA 
AGCCGTTTCG GAAAGTACAT CGACATCCAC TTCAACAAGC GGGGCGCCAT CGAGGGCGCG 
AAGATTGAGC AGTACCTGCT GGAAAAGTCA CGTGTCTGTC GCCAGGCCCT GGATGAAAGG 
AACTACCACG TGTTCTACTG CATGCTGGAG GGTATGAGTG AGGATCAGAA GAAGAAGCTG 
GGCTTGGGCC AGGCCTCTGA CTACAACTAC TTGGCCATGG GTAACTGCAT AACCTGTGAG 
GGCCGGGTGG ACAGCCAGGA GTACGCCAAC ATCCGCTCCG CCATGAAGGT GCTCATGTTC 
ACTGACACCG AGAACTGGGA GATCTCGAAG CTCCTGGCTG CCATCCTGCA CCTGGGCAAC 
CTGCAGTATG AGGCACGCAC ATTTGAAAAC CTGGATGCCT GTGAGGTTCT CTTCTCCCCA 
TCGCTGGCCA CAGCTGCATC CCTGCTTGAG GTGAACCCCC CAGACCTGAT GAGCTGCCTG 
ACTAGCCGCA CCCTCATCAC CCGCGGGGAG ACGGTGTCCA CCCCACTGAG CAGGGAACAG 
GCACTGGACG TGCGCGACGC CTTCGTAAAG GGGATCTACG GGCGGCTGTT CGTGTGGATT 
GTGGACAAGA TCAACGCAGC AATTTACAAG CCTCCCTCCC AGGATGTGAA GAACTCTCGC 
AGGTCCATCG GCCTCCTGGA CATCTTTGGG TTTGAGAACT TTGCTGTGAA CAGCTTTGAG 
CAGCTCTGCA TCAACTTCGC CAATGAGCAC CTGCAGCAGT TCTTTGTGCG GCACGTGTTC 
AAGCTGGAGC AGGAGGAATA TGACCTGGAG AGCATTGACT GGCTGCACAT CGAGTTCACT 
GACAACCAGG ATGCCCTGGA CATGATTGCC AACAAGCCCA TGAACATCAT CTCCCTCATC 
GATGAGGAGA GCAAGTTCCC CAAGGGCACA GACACCACCA TGTTACACAA GCTGAACTCC 
CAGCACAAGC TCAACGCCAA CTACATCCCC CCCAAGAACA ACCATGAGAC CCAGTTTGGC 
ATCAACCATT TTGCAGGCAT CGTCTACTAT GAGACCCAAG GCTTCCTGGA GAAGAACCGA 
GACACCCTGC ATGGGGACAT TATCCAGCTG GTCCACTCCT CCAGGAACAA GTTCATCAAG 
CAGATCTTCC AGGCCGATGT CGCCATGGGC GCCGAGACCA GGAAGCGCTC GCCCACACTT 
AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG CTGATGCGCA CGCTGGGTGC CTGCCAGCCC 
TTCTTTGTGC GATGCATCAA GCCCAATGAG TTCAAGAAGC CCATGCTGTT CGACCGGCAC 
CTGTGCGTGC GCCAGCTGCG GTACTCAGGA ATGATGGAGA CCATCCGAAT CCGCCGAGCT 
GGCTACCCCA TCCGCTACAG CTTCGTAGAG TTTGTGGAGC GGTACCGTGT GCTGCTGCCA 
GGTGTGAAGC CGGCCTACAA GCAGGGCGAC CTCCGCGGGA CTTGCCAGCG CATGGCTGAG 
GCTGTGCTGG GCACCCACGA TGACTGGCAG ATAGGCAAAA CCAAGATCTT TCTGAAGGAC 
CACCATGACA TGCTGCTGGA AGTGGAGCGG GACAAAGCCA TCACCGACAG AGTCATCCTC 
CTTCAGAAAG TCATCCGGGG ATTCAAAGAC AGGTCTAACT TTCTGAAGCT GAAGAACGCT 
GCCACACTGA TCCAGAGGCA CTGGCGGGGT CACAACTGTA GGAAGAACTA CGGGCTGATG 
CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG CACCGCTCCC GGAAGCTGCA CCAGCAGTAC 
CGCCTGGCCC GCCAGCGCAT CATCCAGTTC CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC 
AAGGCCTTCC GCCACCGCCT CTGGGCTGTG CTCACCGTGC AGGCCTATGC CCGGGGCATG 
ATCGCCCGCA GGCTGCACCA ACGCCTCAGG GCTGAGTATC TGTGGCGCCT CGAGGCTGAG 
AAAATGCGGC TGGCGGAGGA AGAGAAGCTT CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG 
GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC CTGGCCCAGC TGGCTCGTGA GGACGCTGAG 
CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG CGGAAGAAGG AGCTCCTGGA GCAGATGGAA 
AGGGCCCGCC ATGAGCCTGT CAATCACTCA GACATGGTGG ACAAGATGTT TGGCTTCCTG 
GGGACTTCAG GTGGCCTGCC AGGCCAGGAG GGCCAGGCAC CTAGTGGCTT TGAGGACCTG 
GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG GACCTGGATG CAGCCCTGCC CCTGCCTGAC 
GAGGATGAGG AGGACCTCTC TGAGTATAAA TTTGCCAAGT TCGCGGCCAC CTACTTCCAG 
GGGACAACCA CGCACTCCTA CACCCGGCGG CCACTCAAAC AGCCACTGCT CTACCATGAC 
GACGAGGGTG ACCAGCTGGC AGCCCTGGCG GTCTGGATCA CCATCCTCCG CTTCATGGGG 
GACCTCCCTG AGCCCAAGTA CCACACAGCC ATGAGTGATG GCAGTGAGAA GATCCCTGTG 
ATGACCAAGA TTTATGAGAC CCTGGGCAAG AAGACGTACA AGAGGGAGCT GCAGGCCCTG 
CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG GGCCAGAAGA AGAGCAGTGT GAGGCACAAG 
CTGGTGCATT TGACTCTGAA AAAGAAGTCC AAGCTCACAG AGGAGGTGAC CAAGAGGCTG 
CATGACGGGG AGTCCACAGT GCAGGGCAAC AGCATGCTGG AGGACCGGCC CACCTCCAAC 
CTGGAGAAGC TGCACTTCAT CATCGGCAAT GGCATCCTGC GGCCAGCACT CCGGGACGAG 
ATCTACTGCC AGATCAGCAA GCAGCTGACC CACAACCCCT CCAAGAGCAG CTATGCCCGG 
GGCTGGATTC TCGTGTCTCT CTGCGTGGGC TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG 
TACCTGCGGA ACTTCATCCA CGGGGGCCCG CCCGGCTACG CCCCGTACTG TGAGGAGCGC 
CTGAGAAGGA CCTTTGTCAA TGGGACACGG ACACAGCCGC CCAGCTGGCT GGAGCTGCAG 
GCCACCAAGT CCAAGAAGCC AATCATGTTG CCCGTGACAT TCATGGATGG GACCACCAAG 
ACCCTGCTGA CGGACTCGGC AACCACGGCC AAGGAGCTCT GCAACGCGCT GGCCGACAAG 
ATCTCTCTCA AGGACCGGTT CGGGTTCTCC CTCTACATTG CCCTGTTTGA CAAGGTGTCC 
TCCCTGGGCA GCGGCAGTGA CCACGTCATG GACGCCATCT CCCAGTGCGA GCAGTACGCC 
AAGGAGCAGG GCGCCCAGGA GCGCAACGCC CCCTGGAGGC TCTTCTTCCG CAAAGAGGTC 
TTCACGCCCT GGCACAGCCC CTCCGAGGAC AACGTGGCCA CCAACCTCAT CTACCAGCAG 
GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC AGGTGTGAGA AGGAGGACGA CCTGGCTGAG 
CTGGCCTCCC AGCAGTACTT TGTAGACTAT GGCTCTGAGA TGATCCTGGA GCGCCTCCTG 
AACCTCGTGC CCACCTACAT CCCCGACCGC GAGATCACGC CCCTGAAGAC GCTGGAGAAG 
TGGGCCCAGC TGGCCATCGC CGCCCACAAG AAGGGGATTT ATGCCCAGAG GAGAACTGAT 
GCCCAGAAGG TCAAAGAGGA TGTGGTCAGT TATGCCCGCT TCAAGTGGCC CTTGCTCTTC 
TCCAGGTTTT ATGAAGCCTA CAAATTCTCA GGCCCCAGTC TCCCCAAGAA CGACGTCATC 
GTGGCCGTCA ACTGGACGGG TGTGTACTTT GTGGATGAGC AGGAGCAGGT ACTTCTGGAG 
CTGTCCTTCC CAGAGATCAT GGCCGTGTCC AGCAGCAGGG AGTGCCGTGT CTGGCTCTCA 
CTGGGCTGCT CTGATCTTGG CTGTGCTGCG CCTCACTCAG GCTGGGCAGG ACTGACCCCG 
GCGGGGCCCT GTTCTCCGTG TTGGTCCTGC AGGGGAGCGA AAACGACGGC CCCCAGCTTC 
ACGCTGGCCA CCATCAAGGG GGACGAATAC ACCTTCACCT CCAGCAATGC TGAGGACATT 
CGTGACCTGG TGGTCACCTT CCTAGAGGGG CTCCGGAAGA GATCTAAGTA TGTTGTGGCC 
CTGCAGGATA ACCCCAACCC CGCAGGCGAG GAGTCAGGCT TCCTCAGCTT TGCCAAGGGA 
GACCTCATCA TCCTGGACCA TGACACGGGC GAGCAGGTCA TGAACTCGGG CTGGGCCAAC 
GGCATCAATG AGAGGACCAA GCAGCGTGGG GACTTCCCCA CCGACAGTGT GTACGTCATG 
CCCACTGTCA CCATGCCACC GCGGGAGATT GTGGCCCTGG TCACCATGAC TCCCGATCAG 
AGGCAGGACG TTGTCCGGCT CTTGCAGCTG CGAACGGCGG AGCCCGAGGT GCGTGCCAAG 
CCCTACACGC TGGAGGAGTT TTCCTATGAC TACTTCAGGC CCCCACCCAA GCACACGCTG 
AGCCGTGTCA TGGTGTCCAA GGCCCGAGGC AAGGACCGGC TGTGGAGCCA CACGCGGGAA 
CCGCTCAAGC AGGCGCTGCT CAAGAAGCTC CTGGGCAGTG AGGAGCTCTC GCAGGAGGCC 
TGCCTGGCCT TCATTGCTGT GCTCAAGTAC ATGGGCGACT ACCCGTCCAA GAGGACACGC 
TCCGTCAACG AGCTCACCGA CCAGATCTTT GAGGGTCCCC TGAAAGCCGA GCCCCTGAAG 
GACGAGGCAT ATGTGCAGAT CCTGAAGCAG CTGACCGACA ACCACATCAG GTACAGCGAG 
GAGCGGGGTT GGGAGCTGCT CTGGCTGTGC ACGGGCCTTT TCCCACCCAG CAACATCCTC 
CTGCCCCACG TGCAGCGCTT CCTGCAGTCC CGAAAGCACT GCCCACTCGC CATCGACTGC 
CTGCAACGGC TCCAGAAAGC CCTGAGAAAC GGGTCCCGGA AGTACCCTCC GCACCTGGTG 
GAGGTGGAGG CCATCCAGCA CAAGACCACC CAGATTTTCC ACAAAGTCTA CTTCCCTGAT 
GACACTGACG AGGCCTTCGA AGTGGAGTCC AGCACCAAGG CCAAGGACTT CTGCCAGAAC 
ATCGCCACCA GGCTGCTCCT CAAGTCCTCA GAGGGATTCA GCCTCTTTGT CAAAATTGCA 
GACAAGGTCC TCAGCGTTCC TGAGAATGAC TTCTTCTTTG ACTTTGTTCG ACACTTGACA 
GACTGGATAA AGAAAGCTCG GCCCATCAAG GACGGAATTG TGCCCTCACT CACCTACCAG 
GTGTTCTTCA TGAAGAAGCT GTGGACCACC ACGGTGCCAG GGAAGGATCC CATGGCCGAT 
TCCATCTTCC ACTATTACCA GGAGTTGCCC AAGTATCTCC GAGGCTACCA CAAGTGCACG 
CGGGAGGAGG TGCTGCAGCT GGGGGCGCTG ATCTACAGGG TCAAGTTCGA GGAGGACAAG 
TCCTACTTCC CCAGCATCCC CAAGCTGCTG CGGGAGCTGG TGCCCCAGGA CCTTATCCGG 
CAGGTCTCAC CTGATGACTG GAAGCGGTCC ATCGTCGCCT ACTTCAACAA GCACGCAGGG 
AAGTCCAAGG AGGAGGCCAA GCTGGCCTTC CTGAAGCTCA TCTTCAAGTG GCCCACCTTT 
GGCTCAGCCT TCTTCGAGGT GAAGGTACAC CATGGGCTTC TCAGAGCAGA GGAGGAGGGG 
AACAGGGCAT TGATAAAGCA CAGAGACTCC TCCCTAGGAC TGTGGGAAAG AGCCATGGCC 
TTGGACACCT GCCCTGGTGA GGGAAGGAGG GCAGCACCCT CAGCCTTGTC CCAGCTTAGC 
GTCTTAGCTG GGGTAAGTCC TGGGGACAGA GGGACCAGAA GAAGCTCAGC CTGGCAGTGG 
AGGCAGACAT GTAAACAGGC TGGGGTAATG GAGGATGGCC TGTGTTCTGA CACTGGGGAG 
CCCTCGCCCA GGTGGCAGAG CTCGGGAGGC TCTGCCGGGT GTGAGAAGGC CTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543348.2
CDS	16..6792
Translation	MEDPADPADPARIPQPGKWALVRSLNQAFKTYAVLPGDHVWMDLRLGQEFDVPIGAVVKLCDSGQVQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNSRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQALDERNYHVFYCMLEGMSEDQKKKLGLGQASDYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHLGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVDKINAAIYKPPSQDVKNSRRSIGLLDIFGFENFAVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQDALDMIANKPMNIISLIDEESKFPKGTDTTMLHKLNSQHKLNANYIPPKNNHETQFGINHFAGIVYYETQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRRAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLKLKNAATLIQRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLHQQYRLARQRIIQFQARCRAYLVRKAFRHRLWAVLTVQAYARGMIARRLHQRLRAEYLWRLEAEKMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEAARRKKELLEQMERARHEPVNHSDMVDKMFGFLGTSGGLPGQEGQAPSGFEDLERGRREMVEEDLDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGEAQLPEGQKKSSVRHKLVHLTLKKKSKLTEEVTKRLHDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTAKELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHSPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLNLVPTYIPDREITPLKTLEKWAQLAIAAHKKGIYAQRRTDAQKVKEDVVSYARFKWPLLFSRFYEAYKFSGPSLPKNDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRECRVWLSLGCSDLGCAAPHSGWAGLTPAGPCSPCWSCRGAKTTAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDSVYVMPTVTMPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKLLGSEELSQEACLAFIAVLKYMGDYPSKRTRSVNELTDQIFEGPLKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIATRLLLKSSEGFSLFVKIADKVLSVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKVHHGLLRAEEEGNRALIKHRDSSLGLWERAMALDTCPGEGRRAAPSALSQLSVLAGVSPGDRGTRRSSAWQWRQTCKQAGVMEDGLCSDTGEPSPRWQSSGGSAGCEKAS

Target ORF information:

RefSeq Version	XM_011545046.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VIIA (MYO7A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545046.2

ORF Insert Sequence:

ATGGAGGACC CCGCCGACCC TGCCGACCCC GCGCGCATCC CGCAGCCCGG CAAGTGGGCG 
CTGGTGCGCA GCCTGAACCA GGCGTTCAAG ACCTACGCCG TCCTGCCGGG GGACCATGTG 
TGGATGGACC TGAGATTGGG GCAGGAGTTC GACGTGCCCA TCGGGGCGGT GGTGAAGCTC 
TGCGACTCTG GGCAGGTCCA GGTGGTGGAT GATGAAGACA ATGAACACTG GATCTCTCCG 
CAGAACGCAA CGCACATCAA GCCTATGCAC CCCACGTCGG TCCACGGCGT GGAGGACATG 
ATCCGCCTGG GGGACCTCAA CGAGGCGGGC ATCTTGCGCA ACCTGCTTAT CCGCTACCGG 
GACCACCTCA TCTACACGTA TACGGGCTCC ATCCTGGTGG CTGTGAACCC CTACCAGCTG 
CTCTCCATCT ACTCGCCAGA GCACATCCGC CAGTATACCA ACAAGAAGAT TGGGGAGATG 
CCCCCCCACA TCTTTGCCAT TGCTGACAAC TGCTACTTCA ACATGAAACG CAACAGCCGA 
GACCAGTGCT GCATCATCAG TGGGGAATCT GGGGCCGGGA AGACGGAGAG CACAAAGCTG 
ATCCTGCAGT TCCTGGCAGC CATCAGTGGG CAGCACTCGT GGATTGAGCA GCAGGTCTTG 
GAGGCCACCC CCATTCTGGA AGCATTTGGG AATGCCAAGA CCATCCGCAA TGACAACTCA 
AGCCGTTTCG GAAAGTACAT CGACATCCAC TTCAACAAGC GGGGCGCCAT CGAGGGCGCG 
AAGATTGAGC AGTACCTGCT GGAAAAGTCA CGTGTCTGTC GCCAGGCCCT GGATGAAAGG 
AACTACCACG TGTTCTACTG CATGCTGGAG GGTATGAGTG AGGATCAGAA GAAGAAGCTG 
GGCTTGGGCC AGGCCTCTGA CTACAACTAC TTGGCCATGG GTAACTGCAT AACCTGTGAG 
GGCCGGGTGG ACAGCCAGGA GTACGCCAAC ATCCGCTCCG CCATGAAGGT GCTCATGTTC 
ACTGACACCG AGAACTGGGA GATCTCGAAG CTCCTGGCTG CCATCCTGCA CCTGGGCAAC 
CTGCAGTATG AGGCACGCAC ATTTGAAAAC CTGGATGCCT GTGAGGTTCT CTTCTCCCCA 
TCGCTGGCCA CAGCTGCATC CCTGCTTGAG GTGAACCCCC CAGACCTGAT GAGCTGCCTG 
ACTAGCCGCA CCCTCATCAC CCGCGGGGAG ACGGTGTCCA CCCCACTGAG CAGGGAACAG 
GCACTGGACG TGCGCGACGC CTTCGTAAAG GGGATCTACG GGCGGCTGTT CGTGTGGATT 
GTGGACAAGA TCAACGCAGC AATTTACAAG CCTCCCTCCC AGGATGTGAA GAACTCTCGC 
AGGTCCATCG GCCTCCTGGA CATCTTTGGG TTTGAGAACT TTGCTGTGAA CAGCTTTGAG 
CAGCTCTGCA TCAACTTCGC CAATGAGCAC CTGCAGCAGT TCTTTGTGCG GCACGTGTTC 
AAGCTGGAGC AGGAGGAATA TGACCTGGAG AGCATTGACT GGCTGCACAT CGAGTTCACT 
GACAACCAGG ATGCCCTGGA CATGATTGCC AACAAGCCCA TGAACATCAT CTCCCTCATC 
GATGAGGAGA GCAAGTTCCC CAAGGGCACA GACACCACCA TGTTACACAA GCTGAACTCC 
CAGCACAAGC TCAACGCCAA CTACATCCCC CCCAAGAACA ACCATGAGAC CCAGTTTGGC 
ATCAACCATT TTGCAGGCAT CGTCTACTAT GAGACCCAAG GCTTCCTGGA GAAGAACCGA 
GACACCCTGC ATGGGGACAT TATCCAGCTG GTCCACTCCT CCAGGAACAA GTTCATCAAG 
CAGATCTTCC AGGCCGATGT CGCCATGGGC GCCGAGACCA GGAAGCGCTC GCCCACACTT 
AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG CTGATGCGCA CGCTGGGTGC CTGCCAGCCC 
TTCTTTGTGC GATGCATCAA GCCCAATGAG TTCAAGAAGC CCATGCTGTT CGACCGGCAC 
CTGTGCGTGC GCCAGCTGCG GTACTCAGGA ATGATGGAGA CCATCCGAAT CCGCCGAGCT 
GGCTACCCCA TCCGCTACAG CTTCGTAGAG TTTGTGGAGC GGTACCGTGT GCTGCTGCCA 
GGTGTGAAGC CGGCCTACAA GCAGGGCGAC CTCCGCGGGA CTTGCCAGCG CATGGCTGAG 
GCTGTGCTGG GCACCCACGA TGACTGGCAG ATAGGCAAAA CCAAGATCTT TCTGAAGGAC 
CACCATGACA TGCTGCTGGA AGTGGAGCGG GACAAAGCCA TCACCGACAG AGTCATCCTC 
CTTCAGAAAG TCATCCGGGG ATTCAAAGAC AGGTCTAACT TTCTGAAGCT GAAGAACGCT 
GCCACACTGA TCCAGAGGCA CTGGCGGGGT CACAACTGTA GGAAGAACTA CGGGCTGATG 
CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG CACCGCTCCC GGAAGCTGCA CCAGCAGTAC 
CGCCTGGCCC GCCAGCGCAT CATCCAGTTC CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC 
AAGGCCTTCC GCCACCGCCT CTGGGCTGTG CTCACCGTGC AGGCCTATGC CCGGGGCATG 
ATCGCCCGCA GGCTGCACCA ACGCCTCAGG GCTGAGTATC TGTGGCGCCT CGAGGCTGAG 
AAAATGCGGC TGGCGGAGGA AGAGAAGCTT CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG 
GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC CTGGCCCAGC TGGCTCGTGA GGACGCTGAG 
CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG CGGAAGAAGG AGCTCCTGGA GCAGATGGAA 
AGGGCCCGCC ATGAGCCTGT CAATCACTCA GACATGGTGG ACAAGATGTT TGGCTTCCTG 
GGGACTTCAG GTGGCCTGCC AGGCCAGGAG GGCCAGGCAC CTAGTGGCTT TGAGGACCTG 
GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG GACCTGGATG CAGCCCTGCC CCTGCCTGAC 
GAGGATGAGG AGGACCTCTC TGAGTATAAA TTTGCCAAGT TCGCGGCCAC CTACTTCCAG 
GGGACAACCA CGCACTCCTA CACCCGGCGG CCACTCAAAC AGCCACTGCT CTACCATGAC 
GACGAGGGTG ACCAGCTGGC AGCCCTGGCG GTCTGGATCA CCATCCTCCG CTTCATGGGG 
GACCTCCCTG AGCCCAAGTA CCACACAGCC ATGAGTGATG GCAGTGAGAA GATCCCTGTG 
ATGACCAAGA TTTATGAGAC CCTGGGCAAG AAGACGTACA AGAGGGAGCT GCAGGCCCTG 
CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG GGCCAGAAGA AGAGCAGTGT GAGGCACAAG 
CTGGTGCATT TGACTCTGAA AAAGAAGTCC AAGCTCACAG AGGAGGTGAC CAAGAGGCTG 
CATGACGGGG AGTCCACAGT GCAGGGCAAC AGCATGCTGG AGGACCGGCC CACCTCCAAC 
CTGGAGAAGC TGCACTTCAT CATCGGCAAT GGCATCCTGC GGCCAGCACT CCGGGACGAG 
ATCTACTGCC AGATCAGCAA GCAGCTGACC CACAACCCCT CCAAGAGCAG CTATGCCCGG 
GGCTGGATTC TCGTGTCTCT CTGCGTGGGC TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG 
TACCTGCGGA ACTTCATCCA CGGGGGCCCG CCCGGCTACG CCCCGTACTG TGAGGAGCGC 
CTGAGAAGGA CCTTTGTCAA TGGGACACGG ACACAGCCGC CCAGCTGGCT GGAGCTGCAG 
GCCACCAAGT CCAAGAAGCC AATCATGTTG CCCGTGACAT TCATGGATGG GACCACCAAG 
ACCCTGCTGA CGGACTCGGC AACCACGGCC AAGGAGCTCT GCAACGCGCT GGCCGACAAG 
ATCTCTCTCA AGGACCGGTT CGGGTTCTCC CTCTACATTG CCCTGTTTGA CAAGGTGTCC 
TCCCTGGGCA GCGGCAGTGA CCACGTCATG GACGCCATCT CCCAGTGCGA GCAGTACGCC 
AAGGAGCAGG GCGCCCAGGA GCGCAACGCC CCCTGGAGGC TCTTCTTCCG CAAAGAGGTC 
TTCACGCCCT GGCACAGCCC CTCCGAGGAC AACGTGGCCA CCAACCTCAT CTACCAGCAG 
GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC AGGTGTGAGA AGGAGGACGA CCTGGCTGAG 
CTGGCCTCCC AGCAGTACTT TGTAGACTAT GGCTCTGAGA TGATCCTGGA GCGCCTCCTG 
AACCTCGTGC CCACCTACAT CCCCGACCGC GAGATCACGC CCCTGAAGAC GCTGGAGAAG 
TGGGCCCAGC TGGCCATCGC CGCCCACAAG AAGGGGATTT ATGCCCAGAG GAGAACTGAT 
GCCCAGAAGG TCAAAGAGGA TGTGGTCAGT TATGCCCGCT TCAAGTGGCC CTTGCTCTTC 
TCCAGGTTTT ATGAAGCCTA CAAATTCTCA GGCCCCAGTC TCCCCAAGAA CGACGTCATC 
GTGGCCGTCA ACTGGACGGG TGTGTACTTT GTGGATGAGC AGGAGCAGGT ACTTCTGGAG 
CTGTCCTTCC CAGAGATCAT GGCCGTGTCC AGCAGCAGGG AGTGCCGTGT CTGGCTCTCA 
CTGGGCTGCT CTGATCTTGG CTGTGCTGCG CCTCACTCAG GCTGGGCAGG ACTGACCCCG 
GCGGGGCCCT GTTCTCCGTG TTGGTCCTGC AGGGGAGCGA AAACGACGGC CCCCAGCTTC 
ACGCTGGCCA CCATCAAGGG GGACGAATAC ACCTTCACCT CCAGCAATGC TGAGGACATT 
CGTGACCTGG TGGTCACCTT CCTAGAGGGG CTCCGGAAGA GATCTAAGTA TGTTGTGGCC 
CTGCAGGATA ACCCCAACCC CGCAGGCGAG GAGTCAGGCT TCCTCAGCTT TGCCAAGGGA 
GACCTCATCA TCCTGGACCA TGACACGGGC GAGCAGGTCA TGAACTCGGG CTGGGCCAAC 
GGCATCAATG AGAGGACCAA GCAGCGTGGG GACTTCCCCA CCGACAGTGT GTACGTCATG 
CCCACTGTCA CCATGCCACC GCGGGAGATT GTGGCCCTGG TCACCATGAC TCCCGATCAG 
AGGCAGGACG TTGTCCGGCT CTTGCAGCTG CGAACGGCGG AGCCCGAGGT GCGTGCCAAG 
CCCTACACGC TGGAGGAGTT TTCCTATGAC TACTTCAGGC CCCCACCCAA GCACACGCTG 
AGCCGTGTCA TGGTGTCCAA GGCCCGAGGC AAGGACCGGC TGTGGAGCCA CACGCGGGAA 
CCGCTCAAGC AGGCGCTGCT CAAGAAGCTC CTGGGCAGTG AGGAGCTCTC GCAGGAGGCC 
TGCCTGGCCT TCATTGCTGT GCTCAAGTAC ATGGGCGACT ACCCGTCCAA GAGGACACGC 
TCCGTCAACG AGCTCACCGA CCAGATCTTT GAGGGTCCCC TGAAAGCCGA GCCCCTGAAG 
GACGAGGCAT ATGTGCAGAT CCTGAAGCAG CTGACCGACA ACCACATCAG GTACAGCGAG 
GAGCGGGGTT GGGAGCTGCT CTGGCTGTGC ACGGGCCTTT TCCCACCCAG CAACATCCTC 
CTGCCCCACG TGCAGCGCTT CCTGCAGTCC CGAAAGCACT GCCCACTCGC CATCGACTGC 
CTGCAACGGC TCCAGAAAGC CCTGAGAAAC GGGTCCCGGA AGTACCCTCC GCACCTGGTG 
GAGGTGGAGG CCATCCAGCA CAAGACCACC CAGATTTTCC ACAAAGTCTA CTTCCCTGAT 
GACACTGACG AGGCCTTCGA AGTGGAGTCC AGCACCAAGG CCAAGGACTT CTGCCAGAAC 
ATCGCCACCA GGCTGCTCCT CAAGTCCTCA GAGGGATTCA GCCTCTTTGT CAAAATTGCA 
GACAAGGTCC TCAGCGTTCC TGAGAATGAC TTCTTCTTTG ACTTTGTTCG ACACTTGACA 
GACTGGATAA AGAAAGCTCG GCCCATCAAG GACGGAATTG TGCCCTCACT CACCTACCAG 
GTGTTCTTCA TGAAGAAGCT GTGGACCACC ACGGTGCCAG GGAAGGATCC CATGGCCGAT 
TCCATCTTCC ACTATTACCA GGAGTTGCCC AAGTATCTCC GAGGCTACCA CAAGTGCACG 
CGGGAGGAGG TGCTGCAGCT GGGGGCGCTG ATCTACAGGG TCAAGTTCGA GGAGGACAAG 
TCCTACTTCC CCAGCATCCC CAAGCTGCTG CGGGAGCTGG TGCCCCAGGA CCTTATCCGG 
CAGGTCTCAC CTGATGACTG GAAGCGGTCC ATCGTCGCCT ACTTCAACAA GCACGCAGGG 
AAGTCCAAGG AGGAGGCCAA GCTGGCCTTC CTGAAGCTCA TCTTCAAGTG GCCCACCTTT 
GGCTCAGCCT TCTTCGAGGT GAAGGTACAC CATGGGCTTC TCAGAGCAGA GGAGGAGGGG 
AACAGGGCAT TGATAAAGCA CAGAGACTCC TCCCTAGGAC TGTGGGAAAG AGCCATGGCC 
TTGGACACCT GCCCTGGTGA GGGAAGGAGG GCAGCACCCT CAGCCTTGTC CCAGCTTAGC 
GTCTTAGCTG GGGTAAGTCC TGGGGACAGA GGGACCAGAA GAAGCTCAGC CTGGCAGTGG 
AGGCAGACAT GTAAACAGGC TGGGGTAATG GAGGATGGCC TGTGTTCTGA CACTGGGGAG 
CCCTCGCCCA GGTGGCAGAG CTCGGGAGGC TCTGCCGGGT GTGAGAAGGC CTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO7A ( XM_017017778.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873267.1 Homo sapiens myosin VIIA (MYO7A), transcript variant X2, mRNA.

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CloneID	OHu92255
Clone ID Related Accession (Same CDS sequence)	XM_017017778.1
Accession Version	XM_017017778.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	unconventional myosin-VIIa isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACC CCGCCGACCC TGCCGACCCC GCGCGCATCC CGCAGCCCGG CAAGTGGGCG 
CTGGTGCGCA GCCTGAACCA GGCGTTCAAG ACCTACGCCG TCCTGCCGGG GGACCATGTG 
TGGATGGACC TGAGATTGGG GCAGGAGTTC GACGTGCCCA TCGGGGCGGT GGTGAAGCTC 
TGCGACTCTG GGCAGGTCCA GGTGGTGGAT GATGAAGACA ATGAACACTG GATCTCTCCG 
CAGAACGCAA CGCACATCAA GCCTATGCAC CCCACGTCGG TCCACGGCGT GGAGGACATG 
ATCCGCCTGG GGGACCTCAA CGAGGCGGGC ATCTTGCGCA ACCTGCTTAT CCGCTACCGG 
GACCACCTCA TCTACACGTA TACGGGCTCC ATCCTGGTGG CTGTGAACCC CTACCAGCTG 
CTCTCCATCT ACTCGCCAGA GCACATCCGC CAGTATACCA ACAAGAAGAT TGGGGAGATG 
CCCCCCCACA TCTTTGCCAT TGCTGACAAC TGCTACTTCA ACATGAAACG CAACAGCCGA 
GACCAGTGCT GCATCATCAG TGGGGAATCT GGGGCCGGGA AGACGGAGAG CACAAAGCTG 
ATCCTGCAGT TCCTGGCAGC CATCAGTGGG CAGCACTCGT GGATTGAGCA GCAGGTCTTG 
GAGGCCACCC CCATTCTGGA AGCATTTGGG AATGCCAAGA CCATCCGCAA TGACAACTCA 
AGCCGTTTCG GAAAGTACAT CGACATCCAC TTCAACAAGC GGGGCGCCAT CGAGGGCGCG 
AAGATTGAGC AGTACCTGCT GGAAAAGTCA CGTGTCTGTC GCCAGGCCCT GGATGAAAGG 
AACTACCACG TGTTCTACTG CATGCTGGAG GGTATGAGTG AGGATCAGAA GAAGAAGCTG 
GGCTTGGGCC AGGCCTCTGA CTACAACTAC TTGGCCATGG GTAACTGCAT AACCTGTGAG 
GGCCGGGTGG ACAGCCAGGA GTACGCCAAC ATCCGCTCCG CCATGAAGGT GCTCATGTTC 
ACTGACACCG AGAACTGGGA GATCTCGAAG CTCCTGGCTG CCATCCTGCA CCTGGGCAAC 
CTGCAGTATG AGGCACGCAC ATTTGAAAAC CTGGATGCCT GTGAGGTTCT CTTCTCCCCA 
TCGCTGGCCA CAGCTGCATC CCTGCTTGAG GTGAACCCCC CAGACCTGAT GAGCTGCCTG 
ACTAGCCGCA CCCTCATCAC CCGCGGGGAG ACGGTGTCCA CCCCACTGAG CAGGGAACAG 
GCACTGGACG TGCGCGACGC CTTCGTAAAG GGGATCTACG GGCGGCTGTT CGTGTGGATT 
GTGGACAAGA TCAACGCAGC AATTTACAAG CCTCCCTCCC AGGATGTGAA GAACTCTCGC 
AGGTCCATCG GCCTCCTGGA CATCTTTGGG TTTGAGAACT TTGCTGTGAA CAGCTTTGAG 
CAGCTCTGCA TCAACTTCGC CAATGAGCAC CTGCAGCAGT TCTTTGTGCG GCACGTGTTC 
AAGCTGGAGC AGGAGGAATA TGACCTGGAG AGCATTGACT GGCTGCACAT CGAGTTCACT 
GACAACCAGG ATGCCCTGGA CATGATTGCC AACAAGCCCA TGAACATCAT CTCCCTCATC 
GATGAGGAGA GCAAGTTCCC CAAGGGCACA GACACCACCA TGTTACACAA GCTGAACTCC 
CAGCACAAGC TCAACGCCAA CTACATCCCC CCCAAGAACA ACCATGAGAC CCAGTTTGGC 
ATCAACCATT TTGCAGGCAT CGTCTACTAT GAGACCCAAG GCTTCCTGGA GAAGAACCGA 
GACACCCTGC ATGGGGACAT TATCCAGCTG GTCCACTCCT CCAGGAACAA GTTCATCAAG 
CAGATCTTCC AGGCCGATGT CGCCATGGGC GCCGAGACCA GGAAGCGCTC GCCCACACTT 
AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG CTGATGCGCA CGCTGGGTGC CTGCCAGCCC 
TTCTTTGTGC GATGCATCAA GCCCAATGAG TTCAAGAAGC CCATGCTGTT CGACCGGCAC 
CTGTGCGTGC GCCAGCTGCG GTACTCAGGA ATGATGGAGA CCATCCGAAT CCGCCGAGCT 
GGCTACCCCA TCCGCTACAG CTTCGTAGAG TTTGTGGAGC GGTACCGTGT GCTGCTGCCA 
GGTGTGAAGC CGGCCTACAA GCAGGGCGAC CTCCGCGGGA CTTGCCAGCG CATGGCTGAG 
GCTGTGCTGG GCACCCACGA TGACTGGCAG ATAGGCAAAA CCAAGATCTT TCTGAAGGAC 
CACCATGACA TGCTGCTGGA AGTGGAGCGG GACAAAGCCA TCACCGACAG AGTCATCCTC 
CTTCAGAAAG TCATCCGGGG ATTCAAAGAC AGGTCTAACT TTCTGAAGCT GAAGAACGCT 
GCCACACTGA TCCAGAGGCA CTGGCGGGGT CACAACTGTA GGAAGAACTA CGGGCTGATG 
CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG CACCGCTCCC GGAAGCTGCA CCAGCAGTAC 
CGCCTGGCCC GCCAGCGCAT CATCCAGTTC CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC 
AAGGCCTTCC GCCACCGCCT CTGGGCTGTG CTCACCGTGC AGGCCTATGC CCGGGGCATG 
ATCGCCCGCA GGCTGCACCA ACGCCTCAGG GCTGAGTATC TGTGGCGCCT CGAGGCTGAG 
AAAATGCGGC TGGCGGAGGA AGAGAAGCTT CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG 
GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC CTGGCCCAGC TGGCTCGTGA GGACGCTGAG 
CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG CGGAAGAAGG AGCTCCTGGA GCAGATGGAA 
AGGGCCCGCC ATGAGCCTGT CAATCACTCA GACATGGTGG ACAAGATGTT TGGCTTCCTG 
GGGACTTCAG GTGGCCTGCC AGGCCAGGAG GGCCAGGCAC CTAGTGGCTT TGAGGACCTG 
GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG GACCTGGATG CAGCCCTGCC CCTGCCTGAC 
GAGGATGAGG AGGACCTCTC TGAGTATAAA TTTGCCAAGT TCGCGGCCAC CTACTTCCAG 
GGGACAACCA CGCACTCCTA CACCCGGCGG CCACTCAAAC AGCCACTGCT CTACCATGAC 
GACGAGGGTG ACCAGCTGGC AGCCCTGGCG GTCTGGATCA CCATCCTCCG CTTCATGGGG 
GACCTCCCTG AGCCCAAGTA CCACACAGCC ATGAGTGATG GCAGTGAGAA GATCCCTGTG 
ATGACCAAGA TTTATGAGAC CCTGGGCAAG AAGACGTACA AGAGGGAGCT GCAGGCCCTG 
CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG GGCCAGAAGA AGAGCAGTGT GAGGCACAAG 
CTGGTGCATT TGACTCTGAA AAAGAAGTCC AAGCTCACAG AGGAGGTGAC CAAGAGGCTG 
CATGACGGGG AGTCCACAGT GCAGGGCAAC AGCATGCTGG AGGACCGGCC CACCTCCAAC 
CTGGAGAAGC TGCACTTCAT CATCGGCAAT GGCATCCTGC GGCCAGCACT CCGGGACGAG 
ATCTACTGCC AGATCAGCAA GCAGCTGACC CACAACCCCT CCAAGAGCAG CTATGCCCGG 
GGCTGGATTC TCGTGTCTCT CTGCGTGGGC TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG 
TACCTGCGGA ACTTCATCCA CGGGGGCCCG CCCGGCTACG CCCCGTACTG TGAGGAGCGC 
CTGAGAAGGA CCTTTGTCAA TGGGACACGG ACACAGCCGC CCAGCTGGCT GGAGCTGCAG 
GCCACCAAGT CCAAGAAGCC AATCATGTTG CCCGTGACAT TCATGGATGG GACCACCAAG 
ACCCTGCTGA CGGACTCGGC AACCACGGCC AAGGAGCTCT GCAACGCGCT GGCCGACAAG 
ATCTCTCTCA AGGACCGGTT CGGGTTCTCC CTCTACATTG CCCTGTTTGA CAAGCAACGT 
CATGCTCAGC AGAGGGGCCC CCGCCCACAA GCCCAGGGGA GGTACCGTGT TGCTGGACAC 
AGTTTGCCTG GATTTTTCTC ACCGATGCTC TTATGTTTCC AGGTGTCCTC CCTGGGCAGC 
GGCAGTGACC ACGTCATGGA CGCCATCTCC CAGTGCGAGC AGTACGCCAA GGAGCAGGGC 
GCCCAGGAGC GCAACGCCCC CTGGAGGCTC TTCTTCCGCA AAGAGGTCTT CACGCCCTGG 
CACAGCCCCT CCGAGGACAA CGTGGCCACC AACCTCATCT ACCAGCAGGT GGTGCGAGGA 
GTCAAGTTTG GGGAGTACAG GTGTGAGAAG GAGGACGACC TGGCTGAGCT GGCCTCCCAG 
CAGTACTTTG TAGACTATGG CTCTGAGATG ATCCTGGAGC GCCTCCTGAA CCTCGTGCCC 
ACCTACATCC CCGACCGCGA GATCACGCCC CTGAAGACGC TGGAGAAGTG GGCCCAGCTG 
GCCATCGCCG CCCACAAGAA GGGGATTTAT GCCCAGAGGA GAACTGATGC CCAGAAGGTC 
AAAGAGGATG TGGTCAGTTA TGCCCGCTTC AAGTGGCCCT TGCTCTTCTC CAGGTTTTAT 
GAAGCCTACA AATTCTCAGG CCCCAGTCTC CCCAAGAACG ACGTCATCGT GGCCGTCAAC 
TGGACGGGTG TGTACTTTGT GGATGAGCAG GAGCAGGTAC TTCTGGAGCT GTCCTTCCCA 
GAGATCATGG CCGTGTCCAG CAGCAGGGGA GCGAAAACGA CGGCCCCCAG CTTCACGCTG 
GCCACCATCA AGGGGGACGA ATACACCTTC ACCTCCAGCA ATGCTGAGGA CATTCGTGAC 
CTGGTGGTCA CCTTCCTAGA GGGGCTCCGG AAGAGATCTA AGTATGTTGT GGCCCTGCAG 
GATAACCCCA ACCCCGCAGG CGAGGAGTCA GGCTTCCTCA GCTTTGCCAA GGGAGACCTC 
ATCATCCTGG ACCATGACAC GGGCGAGCAG GTCATGAACT CGGGCTGGGC CAACGGCATC 
AATGAGAGGA CCAAGCAGCG TGGGGACTTC CCCACCGACA GTGTGTACGT CATGCCCACT 
GTCACCATGC CACCGCGGGA GATTGTGGCC CTGGTCACCA TGACTCCCGA TCAGAGGCAG 
GACGTTGTCC GGCTCTTGCA GCTGCGAACG GCGGAGCCCG AGGTGCGTGC CAAGCCCTAC 
ACGCTGGAGG AGTTTTCCTA TGACTACTTC AGGCCCCCAC CCAAGCACAC GCTGAGCCGT 
GTCATGGTGT CCAAGGCCCG AGGCAAGGAC CGGCTGTGGA GCCACACGCG GGAACCGCTC 
AAGCAGGCGC TGCTCAAGAA GCTCCTGGGC AGTGAGGAGC TCTCGCAGGA GGCCTGCCTG 
GCCTTCATTG CTGTGCTCAA GTACATGGGC GACTACCCGT CCAAGAGGAC ACGCTCCGTC 
AACGAGCTCA CCGACCAGAT CTTTGAGGGT CCCCTGAAAG CCGAGCCCCT GAAGGACGAG 
GCATATGTGC AGATCCTGAA GCAGCTGACC GACAACCACA TCAGGTACAG CGAGGAGCGG 
GGTTGGGAGC TGCTCTGGCT GTGCACGGGC CTTTTCCCAC CCAGCAACAT CCTCCTGCCC 
CACGTGCAGC GCTTCCTGCA GTCCCGAAAG CACTGCCCAC TCGCCATCGA CTGCCTGCAA 
CGGCTCCAGA AAGCCCTGAG AAACGGGTCC CGGAAGTACC CTCCGCACCT GGTGGAGGTG 
GAGGCCATCC AGCACAAGAC CACCCAGATT TTCCACAAAG TCTACTTCCC TGATGACACT 
GACGAGGCCT TCGAAGTGGA GTCCAGCACC AAGGCCAAGG ACTTCTGCCA GAACATCGCC 
ACCAGGCTGC TCCTCAAGTC CTCAGAGGGA TTCAGCCTCT TTGTCAAAAT TGCAGACAAG 
GTCCTCAGCG TTCCTGAGAA TGACTTCTTC TTTGACTTTG TTCGACACTT GACAGACTGG 
ATAAAGAAAG CTCGGCCCAT CAAGGACGGA ATTGTGCCCT CACTCACCTA CCAGGTGTTC 
TTCATGAAGA AGCTGTGGAC CACCACGGTG CCAGGGAAGG ATCCCATGGC CGATTCCATC 
TTCCACTATT ACCAGGAGTT GCCCAAGTAT CTCCGAGGCT ACCACAAGTG CACGCGGGAG 
GAGGTGCTGC AGCTGGGGGC GCTGATCTAC AGGGTCAAGT TCGAGGAGGA CAAGTCCTAC 
TTCCCCAGCA TCCCCAAGCT GCTGCGGGAG CTGGTGCCCC AGGACCTTAT CCGGCAGGTC 
TCACCTGATG ACTGGAAGCG GTCCATCGTC GCCTACTTCA ACAAGCACGC AGGGAAGTCC 
AAGGAGGAGG CCAAGCTGGC CTTCCTGAAG CTCATCTTCA AGTGGCCCAC CTTTGGCTCA 
GCCTTCTTCG AGGTGAAGGT ACACCATGGG CTTCTCAGAG CAGAGGAGGA GGGGAACAGG 
GCATTGATAA AGCACAGAGA CTCCTCCCTA GGACTGTGGG AAAGAGCCAT GGCCTTGGAC 
ACCTGCCCTG GTGAGGGAAG GAGGGCAGCA CCCTCAGCCT TGTCCCAGCT TAGCGTCTTA 
GCTGGGGTAA GTCCTGGGGA CAGAGGGACC AGAAGAAGCT CAGCCTGGCA GTGGAGGCAG 
ACATGTAAAC AGGCTGGGGT AATGGAGGAT GGCCTGTGTT CTGACACTGG GGAGCCCTCG 
CCCAGGTGGC AGAGCTCGGG AGGCTCTGCC GGGTGTGAGA AGGCCTCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873267.1
CDS	16..6786
Translation	MEDPADPADPARIPQPGKWALVRSLNQAFKTYAVLPGDHVWMDLRLGQEFDVPIGAVVKLCDSGQVQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNSRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQALDERNYHVFYCMLEGMSEDQKKKLGLGQASDYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHLGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVDKINAAIYKPPSQDVKNSRRSIGLLDIFGFENFAVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQDALDMIANKPMNIISLIDEESKFPKGTDTTMLHKLNSQHKLNANYIPPKNNHETQFGINHFAGIVYYETQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRRAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLKLKNAATLIQRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLHQQYRLARQRIIQFQARCRAYLVRKAFRHRLWAVLTVQAYARGMIARRLHQRLRAEYLWRLEAEKMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEAARRKKELLEQMERARHEPVNHSDMVDKMFGFLGTSGGLPGQEGQAPSGFEDLERGRREMVEEDLDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGEAQLPEGQKKSSVRHKLVHLTLKKKSKLTEEVTKRLHDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTAKELCNALADKISLKDRFGFSLYIALFDKQRHAQQRGPRPQAQGRYRVAGHSLPGFFSPMLLCFQVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHSPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLNLVPTYIPDREITPLKTLEKWAQLAIAAHKKGIYAQRRTDAQKVKEDVVSYARFKWPLLFSRFYEAYKFSGPSLPKNDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRGAKTTAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDSVYVMPTVTMPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKLLGSEELSQEACLAFIAVLKYMGDYPSKRTRSVNELTDQIFEGPLKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIATRLLLKSSEGFSLFVKIADKVLSVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKVHHGLLRAEEEGNRALIKHRDSSLGLWERAMALDTCPGEGRRAAPSALSQLSVLAGVSPGDRGTRRSSAWQWRQTCKQAGVMEDGLCSDTGEPSPRWQSSGGSAGCEKAS

Target ORF information:

RefSeq Version	XM_017017778.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VIIA (MYO7A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017778.1

ORF Insert Sequence:

ATGGAGGACC CCGCCGACCC TGCCGACCCC GCGCGCATCC CGCAGCCCGG CAAGTGGGCG 
CTGGTGCGCA GCCTGAACCA GGCGTTCAAG ACCTACGCCG TCCTGCCGGG GGACCATGTG 
TGGATGGACC TGAGATTGGG GCAGGAGTTC GACGTGCCCA TCGGGGCGGT GGTGAAGCTC 
TGCGACTCTG GGCAGGTCCA GGTGGTGGAT GATGAAGACA ATGAACACTG GATCTCTCCG 
CAGAACGCAA CGCACATCAA GCCTATGCAC CCCACGTCGG TCCACGGCGT GGAGGACATG 
ATCCGCCTGG GGGACCTCAA CGAGGCGGGC ATCTTGCGCA ACCTGCTTAT CCGCTACCGG 
GACCACCTCA TCTACACGTA TACGGGCTCC ATCCTGGTGG CTGTGAACCC CTACCAGCTG 
CTCTCCATCT ACTCGCCAGA GCACATCCGC CAGTATACCA ACAAGAAGAT TGGGGAGATG 
CCCCCCCACA TCTTTGCCAT TGCTGACAAC TGCTACTTCA ACATGAAACG CAACAGCCGA 
GACCAGTGCT GCATCATCAG TGGGGAATCT GGGGCCGGGA AGACGGAGAG CACAAAGCTG 
ATCCTGCAGT TCCTGGCAGC CATCAGTGGG CAGCACTCGT GGATTGAGCA GCAGGTCTTG 
GAGGCCACCC CCATTCTGGA AGCATTTGGG AATGCCAAGA CCATCCGCAA TGACAACTCA 
AGCCGTTTCG GAAAGTACAT CGACATCCAC TTCAACAAGC GGGGCGCCAT CGAGGGCGCG 
AAGATTGAGC AGTACCTGCT GGAAAAGTCA CGTGTCTGTC GCCAGGCCCT GGATGAAAGG 
AACTACCACG TGTTCTACTG CATGCTGGAG GGTATGAGTG AGGATCAGAA GAAGAAGCTG 
GGCTTGGGCC AGGCCTCTGA CTACAACTAC TTGGCCATGG GTAACTGCAT AACCTGTGAG 
GGCCGGGTGG ACAGCCAGGA GTACGCCAAC ATCCGCTCCG CCATGAAGGT GCTCATGTTC 
ACTGACACCG AGAACTGGGA GATCTCGAAG CTCCTGGCTG CCATCCTGCA CCTGGGCAAC 
CTGCAGTATG AGGCACGCAC ATTTGAAAAC CTGGATGCCT GTGAGGTTCT CTTCTCCCCA 
TCGCTGGCCA CAGCTGCATC CCTGCTTGAG GTGAACCCCC CAGACCTGAT GAGCTGCCTG 
ACTAGCCGCA CCCTCATCAC CCGCGGGGAG ACGGTGTCCA CCCCACTGAG CAGGGAACAG 
GCACTGGACG TGCGCGACGC CTTCGTAAAG GGGATCTACG GGCGGCTGTT CGTGTGGATT 
GTGGACAAGA TCAACGCAGC AATTTACAAG CCTCCCTCCC AGGATGTGAA GAACTCTCGC 
AGGTCCATCG GCCTCCTGGA CATCTTTGGG TTTGAGAACT TTGCTGTGAA CAGCTTTGAG 
CAGCTCTGCA TCAACTTCGC CAATGAGCAC CTGCAGCAGT TCTTTGTGCG GCACGTGTTC 
AAGCTGGAGC AGGAGGAATA TGACCTGGAG AGCATTGACT GGCTGCACAT CGAGTTCACT 
GACAACCAGG ATGCCCTGGA CATGATTGCC AACAAGCCCA TGAACATCAT CTCCCTCATC 
GATGAGGAGA GCAAGTTCCC CAAGGGCACA GACACCACCA TGTTACACAA GCTGAACTCC 
CAGCACAAGC TCAACGCCAA CTACATCCCC CCCAAGAACA ACCATGAGAC CCAGTTTGGC 
ATCAACCATT TTGCAGGCAT CGTCTACTAT GAGACCCAAG GCTTCCTGGA GAAGAACCGA 
GACACCCTGC ATGGGGACAT TATCCAGCTG GTCCACTCCT CCAGGAACAA GTTCATCAAG 
CAGATCTTCC AGGCCGATGT CGCCATGGGC GCCGAGACCA GGAAGCGCTC GCCCACACTT 
AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG CTGATGCGCA CGCTGGGTGC CTGCCAGCCC 
TTCTTTGTGC GATGCATCAA GCCCAATGAG TTCAAGAAGC CCATGCTGTT CGACCGGCAC 
CTGTGCGTGC GCCAGCTGCG GTACTCAGGA ATGATGGAGA CCATCCGAAT CCGCCGAGCT 
GGCTACCCCA TCCGCTACAG CTTCGTAGAG TTTGTGGAGC GGTACCGTGT GCTGCTGCCA 
GGTGTGAAGC CGGCCTACAA GCAGGGCGAC CTCCGCGGGA CTTGCCAGCG CATGGCTGAG 
GCTGTGCTGG GCACCCACGA TGACTGGCAG ATAGGCAAAA CCAAGATCTT TCTGAAGGAC 
CACCATGACA TGCTGCTGGA AGTGGAGCGG GACAAAGCCA TCACCGACAG AGTCATCCTC 
CTTCAGAAAG TCATCCGGGG ATTCAAAGAC AGGTCTAACT TTCTGAAGCT GAAGAACGCT 
GCCACACTGA TCCAGAGGCA CTGGCGGGGT CACAACTGTA GGAAGAACTA CGGGCTGATG 
CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG CACCGCTCCC GGAAGCTGCA CCAGCAGTAC 
CGCCTGGCCC GCCAGCGCAT CATCCAGTTC CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC 
AAGGCCTTCC GCCACCGCCT CTGGGCTGTG CTCACCGTGC AGGCCTATGC CCGGGGCATG 
ATCGCCCGCA GGCTGCACCA ACGCCTCAGG GCTGAGTATC TGTGGCGCCT CGAGGCTGAG 
AAAATGCGGC TGGCGGAGGA AGAGAAGCTT CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG 
GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC CTGGCCCAGC TGGCTCGTGA GGACGCTGAG 
CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG CGGAAGAAGG AGCTCCTGGA GCAGATGGAA 
AGGGCCCGCC ATGAGCCTGT CAATCACTCA GACATGGTGG ACAAGATGTT TGGCTTCCTG 
GGGACTTCAG GTGGCCTGCC AGGCCAGGAG GGCCAGGCAC CTAGTGGCTT TGAGGACCTG 
GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG GACCTGGATG CAGCCCTGCC CCTGCCTGAC 
GAGGATGAGG AGGACCTCTC TGAGTATAAA TTTGCCAAGT TCGCGGCCAC CTACTTCCAG 
GGGACAACCA CGCACTCCTA CACCCGGCGG CCACTCAAAC AGCCACTGCT CTACCATGAC 
GACGAGGGTG ACCAGCTGGC AGCCCTGGCG GTCTGGATCA CCATCCTCCG CTTCATGGGG 
GACCTCCCTG AGCCCAAGTA CCACACAGCC ATGAGTGATG GCAGTGAGAA GATCCCTGTG 
ATGACCAAGA TTTATGAGAC CCTGGGCAAG AAGACGTACA AGAGGGAGCT GCAGGCCCTG 
CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG GGCCAGAAGA AGAGCAGTGT GAGGCACAAG 
CTGGTGCATT TGACTCTGAA AAAGAAGTCC AAGCTCACAG AGGAGGTGAC CAAGAGGCTG 
CATGACGGGG AGTCCACAGT GCAGGGCAAC AGCATGCTGG AGGACCGGCC CACCTCCAAC 
CTGGAGAAGC TGCACTTCAT CATCGGCAAT GGCATCCTGC GGCCAGCACT CCGGGACGAG 
ATCTACTGCC AGATCAGCAA GCAGCTGACC CACAACCCCT CCAAGAGCAG CTATGCCCGG 
GGCTGGATTC TCGTGTCTCT CTGCGTGGGC TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG 
TACCTGCGGA ACTTCATCCA CGGGGGCCCG CCCGGCTACG CCCCGTACTG TGAGGAGCGC 
CTGAGAAGGA CCTTTGTCAA TGGGACACGG ACACAGCCGC CCAGCTGGCT GGAGCTGCAG 
GCCACCAAGT CCAAGAAGCC AATCATGTTG CCCGTGACAT TCATGGATGG GACCACCAAG 
ACCCTGCTGA CGGACTCGGC AACCACGGCC AAGGAGCTCT GCAACGCGCT GGCCGACAAG 
ATCTCTCTCA AGGACCGGTT CGGGTTCTCC CTCTACATTG CCCTGTTTGA CAAGCAACGT 
CATGCTCAGC AGAGGGGCCC CCGCCCACAA GCCCAGGGGA GGTACCGTGT TGCTGGACAC 
AGTTTGCCTG GATTTTTCTC ACCGATGCTC TTATGTTTCC AGGTGTCCTC CCTGGGCAGC 
GGCAGTGACC ACGTCATGGA CGCCATCTCC CAGTGCGAGC AGTACGCCAA GGAGCAGGGC 
GCCCAGGAGC GCAACGCCCC CTGGAGGCTC TTCTTCCGCA AAGAGGTCTT CACGCCCTGG 
CACAGCCCCT CCGAGGACAA CGTGGCCACC AACCTCATCT ACCAGCAGGT GGTGCGAGGA 
GTCAAGTTTG GGGAGTACAG GTGTGAGAAG GAGGACGACC TGGCTGAGCT GGCCTCCCAG 
CAGTACTTTG TAGACTATGG CTCTGAGATG ATCCTGGAGC GCCTCCTGAA CCTCGTGCCC 
ACCTACATCC CCGACCGCGA GATCACGCCC CTGAAGACGC TGGAGAAGTG GGCCCAGCTG 
GCCATCGCCG CCCACAAGAA GGGGATTTAT GCCCAGAGGA GAACTGATGC CCAGAAGGTC 
AAAGAGGATG TGGTCAGTTA TGCCCGCTTC AAGTGGCCCT TGCTCTTCTC CAGGTTTTAT 
GAAGCCTACA AATTCTCAGG CCCCAGTCTC CCCAAGAACG ACGTCATCGT GGCCGTCAAC 
TGGACGGGTG TGTACTTTGT GGATGAGCAG GAGCAGGTAC TTCTGGAGCT GTCCTTCCCA 
GAGATCATGG CCGTGTCCAG CAGCAGGGGA GCGAAAACGA CGGCCCCCAG CTTCACGCTG 
GCCACCATCA AGGGGGACGA ATACACCTTC ACCTCCAGCA ATGCTGAGGA CATTCGTGAC 
CTGGTGGTCA CCTTCCTAGA GGGGCTCCGG AAGAGATCTA AGTATGTTGT GGCCCTGCAG 
GATAACCCCA ACCCCGCAGG CGAGGAGTCA GGCTTCCTCA GCTTTGCCAA GGGAGACCTC 
ATCATCCTGG ACCATGACAC GGGCGAGCAG GTCATGAACT CGGGCTGGGC CAACGGCATC 
AATGAGAGGA CCAAGCAGCG TGGGGACTTC CCCACCGACA GTGTGTACGT CATGCCCACT 
GTCACCATGC CACCGCGGGA GATTGTGGCC CTGGTCACCA TGACTCCCGA TCAGAGGCAG 
GACGTTGTCC GGCTCTTGCA GCTGCGAACG GCGGAGCCCG AGGTGCGTGC CAAGCCCTAC 
ACGCTGGAGG AGTTTTCCTA TGACTACTTC AGGCCCCCAC CCAAGCACAC GCTGAGCCGT 
GTCATGGTGT CCAAGGCCCG AGGCAAGGAC CGGCTGTGGA GCCACACGCG GGAACCGCTC 
AAGCAGGCGC TGCTCAAGAA GCTCCTGGGC AGTGAGGAGC TCTCGCAGGA GGCCTGCCTG 
GCCTTCATTG CTGTGCTCAA GTACATGGGC GACTACCCGT CCAAGAGGAC ACGCTCCGTC 
AACGAGCTCA CCGACCAGAT CTTTGAGGGT CCCCTGAAAG CCGAGCCCCT GAAGGACGAG 
GCATATGTGC AGATCCTGAA GCAGCTGACC GACAACCACA TCAGGTACAG CGAGGAGCGG 
GGTTGGGAGC TGCTCTGGCT GTGCACGGGC CTTTTCCCAC CCAGCAACAT CCTCCTGCCC 
CACGTGCAGC GCTTCCTGCA GTCCCGAAAG CACTGCCCAC TCGCCATCGA CTGCCTGCAA 
CGGCTCCAGA AAGCCCTGAG AAACGGGTCC CGGAAGTACC CTCCGCACCT GGTGGAGGTG 
GAGGCCATCC AGCACAAGAC CACCCAGATT TTCCACAAAG TCTACTTCCC TGATGACACT 
GACGAGGCCT TCGAAGTGGA GTCCAGCACC AAGGCCAAGG ACTTCTGCCA GAACATCGCC 
ACCAGGCTGC TCCTCAAGTC CTCAGAGGGA TTCAGCCTCT TTGTCAAAAT TGCAGACAAG 
GTCCTCAGCG TTCCTGAGAA TGACTTCTTC TTTGACTTTG TTCGACACTT GACAGACTGG 
ATAAAGAAAG CTCGGCCCAT CAAGGACGGA ATTGTGCCCT CACTCACCTA CCAGGTGTTC 
TTCATGAAGA AGCTGTGGAC CACCACGGTG CCAGGGAAGG ATCCCATGGC CGATTCCATC 
TTCCACTATT ACCAGGAGTT GCCCAAGTAT CTCCGAGGCT ACCACAAGTG CACGCGGGAG 
GAGGTGCTGC AGCTGGGGGC GCTGATCTAC AGGGTCAAGT TCGAGGAGGA CAAGTCCTAC 
TTCCCCAGCA TCCCCAAGCT GCTGCGGGAG CTGGTGCCCC AGGACCTTAT CCGGCAGGTC 
TCACCTGATG ACTGGAAGCG GTCCATCGTC GCCTACTTCA ACAAGCACGC AGGGAAGTCC 
AAGGAGGAGG CCAAGCTGGC CTTCCTGAAG CTCATCTTCA AGTGGCCCAC CTTTGGCTCA 
GCCTTCTTCG AGGTGAAGGT ACACCATGGG CTTCTCAGAG CAGAGGAGGA GGGGAACAGG 
GCATTGATAA AGCACAGAGA CTCCTCCCTA GGACTGTGGG AAAGAGCCAT GGCCTTGGAC 
ACCTGCCCTG GTGAGGGAAG GAGGGCAGCA CCCTCAGCCT TGTCCCAGCT TAGCGTCTTA 
GCTGGGGTAA GTCCTGGGGA CAGAGGGACC AGAAGAAGCT CAGCCTGGCA GTGGAGGCAG 
ACATGTAAAC AGGCTGGGGT AATGGAGGAT GGCCTGTGTT CTGACACTGG GGAGCCCTCG 
CCCAGGTGGC AGAGCTCGGG AGGCTCTGCC GGGTGTGAGA AGGCCTCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO7A ( XM_017017779.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873268.1 Homo sapiens myosin VIIA (MYO7A), transcript variant X3, mRNA.

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CloneID	OHu92256
Clone ID Related Accession (Same CDS sequence)	XM_017017779.1
Accession Version	XM_017017779.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	unconventional myosin-VIIa isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACC CCGCCGACCC TGCCGACCCC GCGCGCATCC CGCAGCCCGG CAAGTGGGCG 
CTGGTGCGCA GCCTGAACCA GGCGTTCAAG ACCTACGCCG TCCTGCCGGG GGACCATGTG 
TGGATGGACC TGAGATTGGG GCAGGAGTTC GACGTGCCCA TCGGGGCGGT GGTGAAGCTC 
TGCGACTCTG GGCAGGTCCA GGTGGTGGAT GATGAAGACA ATGAACACTG GATCTCTCCG 
CAGAACGCAA CGCACATCAA GCCTATGCAC CCCACGTCGG TCCACGGCGT GGAGGACATG 
ATCCGCCTGG GGGACCTCAA CGAGGCGGGC ATCTTGCGCA ACCTGCTTAT CCGCTACCGG 
GACCACCTCA TCTACACGTA TACGGGCTCC ATCCTGGTGG CTGTGAACCC CTACCAGCTG 
CTCTCCATCT ACTCGCCAGA GCACATCCGC CAGTATACCA ACAAGAAGAT TGGGGAGATG 
CCCCCCCACA TCTTTGCCAT TGCTGACAAC TGCTACTTCA ACATGAAACG CAACAGCCGA 
GACCAGTGCT GCATCATCAG TGGGGAATCT GGGGCCGGGA AGACGGAGAG CACAAAGCTG 
ATCCTGCAGT TCCTGGCAGC CATCAGTGGG CAGCACTCGT GGATTGAGCA GCAGGTCTTG 
GAGGCCACCC CCATTCTGGA AGCATTTGGG AATGCCAAGA CCATCCGCAA TGACAACTCA 
AGCCGTTTCG GAAAGTACAT CGACATCCAC TTCAACAAGC GGGGCGCCAT CGAGGGCGCG 
AAGATTGAGC AGTACCTGCT GGAAAAGTCA CGTGTCTGTC GCCAGGCCCT GGATGAAAGG 
AACTACCACG TGTTCTACTG CATGCTGGAG GGTATGAGTG AGGATCAGAA GAAGAAGCTG 
GGCTTGGGCC AGGCCTCTGA CTACAACTAC TTGGCCATGG GTAACTGCAT AACCTGTGAG 
GGCCGGGTGG ACAGCCAGGA GTACGCCAAC ATCCGCTCCG CCATGAAGGT GCTCATGTTC 
ACTGACACCG AGAACTGGGA GATCTCGAAG CTCCTGGCTG CCATCCTGCA CCTGGGCAAC 
CTGCAGTATG AGGCACGCAC ATTTGAAAAC CTGGATGCCT GTGAGGTTCT CTTCTCCCCA 
TCGCTGGCCA CAGCTGCATC CCTGCTTGAG GTGAACCCCC CAGACCTGAT GAGCTGCCTG 
ACTAGCCGCA CCCTCATCAC CCGCGGGGAG ACGGTGTCCA CCCCACTGAG CAGGGAACAG 
GCACTGGACG TGCGCGACGC CTTCGTAAAG GGGATCTACG GGCGGCTGTT CGTGTGGATT 
GTGGACAAGA TCAACGCAGC AATTTACAAG CCTCCCTCCC AGGATGTGAA GAACTCTCGC 
AGGTCCATCG GCCTCCTGGA CATCTTTGGG TTTGAGAACT TTGCTGTGAA CAGCTTTGAG 
CAGCTCTGCA TCAACTTCGC CAATGAGCAC CTGCAGCAGT TCTTTGTGCG GCACGTGTTC 
AAGCTGGAGC AGGAGGAATA TGACCTGGAG AGCATTGACT GGCTGCACAT CGAGTTCACT 
GACAACCAGG ATGCCCTGGA CATGATTGCC AACAAGCCCA TGAACATCAT CTCCCTCATC 
GATGAGGAGA GCAAGTTCCC CAAGGGCACA GACACCACCA TGTTACACAA GCTGAACTCC 
CAGCACAAGC TCAACGCCAA CTACATCCCC CCCAAGAACA ACCATGAGAC CCAGTTTGGC 
ATCAACCATT TTGCAGGCAT CGTCTACTAT GAGACCCAAG GCTTCCTGGA GAAGAACCGA 
GACACCCTGC ATGGGGACAT TATCCAGCTG GTCCACTCCT CCAGGAACAA GTTCATCAAG 
CAGATCTTCC AGGCCGATGT CGCCATGGGC GCCGAGACCA GGAAGCGCTC GCCCACACTT 
AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG CTGATGCGCA CGCTGGGTGC CTGCCAGCCC 
TTCTTTGTGC GATGCATCAA GCCCAATGAG TTCAAGAAGC CCATGCTGTT CGACCGGCAC 
CTGTGCGTGC GCCAGCTGCG GTACTCAGGA ATGATGGAGA CCATCCGAAT CCGCCGAGCT 
GGCTACCCCA TCCGCTACAG CTTCGTAGAG TTTGTGGAGC GGTACCGTGT GCTGCTGCCA 
GGTGTGAAGC CGGCCTACAA GCAGGGCGAC CTCCGCGGGA CTTGCCAGCG CATGGCTGAG 
GCTGTGCTGG GCACCCACGA TGACTGGCAG ATAGGCAAAA CCAAGATCTT TCTGAAGGAC 
CACCATGACA TGCTGCTGGA AGTGGAGCGG GACAAAGCCA TCACCGACAG AGTCATCCTC 
CTTCAGAAAG TCATCCGGGG ATTCAAAGAC AGGTCTAACT TTCTGAAGCT GAAGAACGCT 
GCCACACTGA TCCAGAGGCA CTGGCGGGGT CACAACTGTA GGAAGAACTA CGGGCTGATG 
CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG CACCGCTCCC GGAAGCTGCA CCAGCAGTAC 
CGCCTGGCCC GCCAGCGCAT CATCCAGTTC CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC 
AAGGCCTTCC GCCACCGCCT CTGGGCTGTG CTCACCGTGC AGGCCTATGC CCGGGGCATG 
ATCGCCCGCA GGCTGCACCA ACGCCTCAGG GCTGAGTATC TGTGGCGCCT CGAGGCTGAG 
AAAATGCGGC TGGCGGAGGA AGAGAAGCTT CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG 
GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC CTGGCCCAGC TGGCTCGTGA GGACGCTGAG 
CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG CGGAAGAAGG AGCTCCTGGA GCAGATGGAA 
AGGGCCCGCC ATGAGCCTGT CAATCACTCA GACATGGTGG ACAAGATGTT TGGCTTCCTG 
GGGACTTCAG GTGGCCTGCC AGGCCAGGAG GGCCAGGCAC CTAGTGGCTT TGAGGACCTG 
GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG GACCTGGATG CAGCCCTGCC CCTGCCTGAC 
GAGGATGAGG AGGACCTCTC TGAGTATAAA TTTGCCAAGT TCGCGGCCAC CTACTTCCAG 
GGGACAACCA CGCACTCCTA CACCCGGCGG CCACTCAAAC AGCCACTGCT CTACCATGAC 
GACGAGGGTG ACCAGCTGGC AGCCCTGGCG GTCTGGATCA CCATCCTCCG CTTCATGGGG 
GACCTCCCTG AGCCCAAGTA CCACACAGCC ATGAGTGATG GCAGTGAGAA GATCCCTGTG 
ATGACCAAGA TTTATGAGAC CCTGGGCAAG AAGACGTACA AGAGGGAGCT GCAGGCCCTG 
CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG GGCCAGAAGA AGAGCAGTGT GAGGCACAAG 
CTGGTGCATT TGACTCTGAA AAAGAAGTCC AAGCTCACAG AGGAGGTGAC CAAGAGGCTG 
CATGACGGGG AGTCCACAGT GCAGGGCAAC AGCATGCTGG AGGACCGGCC CACCTCCAAC 
CTGGAGAAGC TGCACTTCAT CATCGGCAAT GGCATCCTGC GGCCAGCACT CCGGGACGAG 
ATCTACTGCC AGATCAGCAA GCAGCTGACC CACAACCCCT CCAAGAGCAG CTATGCCCGG 
GGCTGGATTC TCGTGTCTCT CTGCGTGGGC TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG 
TACCTGCGGA ACTTCATCCA CGGGGGCCCG CCCGGCTACG CCCCGTACTG TGAGGAGCGC 
CTGAGAAGGA CCTTTGTCAA TGGGACACGG ACACAGCCGC CCAGCTGGCT GGAGCTGCAG 
GCCACCAAGT CCAAGAAGCC AATCATGTTG CCCGTGACAT TCATGGATGG GACCACCAAG 
ACCCTGCTGA CGGACTCGGC AACCACGGCC AAGGAGCTCT GCAACGCGCT GGCCGACAAG 
ATCTCTCTCA AGGACCGGTT CGGGTTCTCC CTCTACATTG CCCTGTTTGA CAAGCAACGT 
CATGCTCAGC AGAGGGGCCC CCGCCCACAA GCCCAGGGGA GGTACCGTGT TGCTGGACAC 
AGTTTGCCTG GATTTTTCTC ACCGATGCTC TTATGTTTCC AGGTGTCCTC CCTGGGCAGC 
GGCAGTGACC ACGTCATGGA CGCCATCTCC CAGTGCGAGC AGTACGCCAA GGAGCAGGGC 
GCCCAGGAGC GCAACGCCCC CTGGAGGCTC TTCTTCCGCA AAGAGGTCTT CACGCCCTGG 
CACAGCCCCT CCGAGGACAA CGTGGCCACC AACCTCATCT ACCAGCAGGT GGTGCGAGGA 
GTCAAGTTTG GGGAGTACAG GTGTGAGAAG GAGGACGACC TGGCTGAGCT GGCCTCCCAG 
CAGTACTTTG TAGACTATGG CTCTGAGATG ATCCTGGAGC GCCTCCTGAA CCTCGTGCCC 
ACCTACATCC CCGACCGCGA GATCACGCCC CTGAAGACGC TGGAGAAGTG GGCCCAGCTG 
GCCATCGCCG CCCACAAGAA GGGGATTTAT GCCCAGAGGA GAACTGATGC CCAGAAGGTC 
AAAGAGGATG TGGTCAGTTA TGCCCGCTTC AAGTGGCCCT TGCTCTTCTC CAGGTTTTAT 
GAAGCCTACA AATTCTCAGG CCCCAGTCTC CCCAAGAACG ACGTCATCGT GGCCGTCAAC 
TGGACGGGTG TGTACTTTGT GGATGAGCAG GAGCAGGTAC TTCTGGAGCT GTCCTTCCCA 
GAGATCATGG CCGTGTCCAG CAGCAGGGGA GCGAAAACGA CGGCCCCCAG CTTCACGCTG 
GCCACCATCA AGGGGGACGA ATACACCTTC ACCTCCAGCA ATGCTGAGGA CATTCGTGAC 
CTGGTGGTCA CCTTCCTAGA GGGGCTCCGG AAGAGATCTA AGTATGTTGT GGCCCTGCAG 
GATAACCCCA ACCCCGGCGA GGAGTCAGGC TTCCTCAGCT TTGCCAAGGG AGACCTCATC 
ATCCTGGACC ATGACACGGG CGAGCAGGTC ATGAACTCGG GCTGGGCCAA CGGCATCAAT 
GAGAGGACCA AGCAGCGTGG GGACTTCCCC ACCGACAGTG TGTACGTCAT GCCCACTGTC 
ACCATGCCAC CGCGGGAGAT TGTGGCCCTG GTCACCATGA CTCCCGATCA GAGGCAGGAC 
GTTGTCCGGC TCTTGCAGCT GCGAACGGCG GAGCCCGAGG TGCGTGCCAA GCCCTACACG 
CTGGAGGAGT TTTCCTATGA CTACTTCAGG CCCCCACCCA AGCACACGCT GAGCCGTGTC 
ATGGTGTCCA AGGCCCGAGG CAAGGACCGG CTGTGGAGCC ACACGCGGGA ACCGCTCAAG 
CAGGCGCTGC TCAAGAAGCT CCTGGGCAGT GAGGAGCTCT CGCAGGAGGC CTGCCTGGCC 
TTCATTGCTG TGCTCAAGTA CATGGGCGAC TACCCGTCCA AGAGGACACG CTCCGTCAAC 
GAGCTCACCG ACCAGATCTT TGAGGGTCCC CTGAAAGCCG AGCCCCTGAA GGACGAGGCA 
TATGTGCAGA TCCTGAAGCA GCTGACCGAC AACCACATCA GGTACAGCGA GGAGCGGGGT 
TGGGAGCTGC TCTGGCTGTG CACGGGCCTT TTCCCACCCA GCAACATCCT CCTGCCCCAC 
GTGCAGCGCT TCCTGCAGTC CCGAAAGCAC TGCCCACTCG CCATCGACTG CCTGCAACGG 
CTCCAGAAAG CCCTGAGAAA CGGGTCCCGG AAGTACCCTC CGCACCTGGT GGAGGTGGAG 
GCCATCCAGC ACAAGACCAC CCAGATTTTC CACAAAGTCT ACTTCCCTGA TGACACTGAC 
GAGGCCTTCG AAGTGGAGTC CAGCACCAAG GCCAAGGACT TCTGCCAGAA CATCGCCACC 
AGGCTGCTCC TCAAGTCCTC AGAGGGATTC AGCCTCTTTG TCAAAATTGC AGACAAGGTC 
CTCAGCGTTC CTGAGAATGA CTTCTTCTTT GACTTTGTTC GACACTTGAC AGACTGGATA 
AAGAAAGCTC GGCCCATCAA GGACGGAATT GTGCCCTCAC TCACCTACCA GGTGTTCTTC 
ATGAAGAAGC TGTGGACCAC CACGGTGCCA GGGAAGGATC CCATGGCCGA TTCCATCTTC 
CACTATTACC AGGAGTTGCC CAAGTATCTC CGAGGCTACC ACAAGTGCAC GCGGGAGGAG 
GTGCTGCAGC TGGGGGCGCT GATCTACAGG GTCAAGTTCG AGGAGGACAA GTCCTACTTC 
CCCAGCATCC CCAAGCTGCT GCGGGAGCTG GTGCCCCAGG ACCTTATCCG GCAGGTCTCA 
CCTGATGACT GGAAGCGGTC CATCGTCGCC TACTTCAACA AGCACGCAGG GAAGTCCAAG 
GAGGAGGCCA AGCTGGCCTT CCTGAAGCTC ATCTTCAAGT GGCCCACCTT TGGCTCAGCC 
TTCTTCGAGG TGAAGGTACA CCATGGGCTT CTCAGAGCAG AGGAGGAGGG GAACAGGGCA 
TTGATAAAGC ACAGAGACTC CTCCCTAGGA CTGTGGGAAA GAGCCATGGC CTTGGACACC 
TGCCCTGGTG AGGGAAGGAG GGCAGCACCC TCAGCCTTGT CCCAGCTTAG CGTCTTAGCT 
GGGGTAAGTC CTGGGGACAG AGGGACCAGA AGAAGCTCAG CCTGGCAGTG GAGGCAGACA 
TGTAAACAGG CTGGGGTAAT GGAGGATGGC CTGTGTTCTG ACACTGGGGA GCCCTCGCCC 
AGGTGGCAGA GCTCGGGAGG CTCTGCCGGG TGTGAGAAGG CCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873268.1
CDS	16..6783
Translation	MEDPADPADPARIPQPGKWALVRSLNQAFKTYAVLPGDHVWMDLRLGQEFDVPIGAVVKLCDSGQVQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNSRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQALDERNYHVFYCMLEGMSEDQKKKLGLGQASDYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHLGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVDKINAAIYKPPSQDVKNSRRSIGLLDIFGFENFAVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQDALDMIANKPMNIISLIDEESKFPKGTDTTMLHKLNSQHKLNANYIPPKNNHETQFGINHFAGIVYYETQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRRAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLKLKNAATLIQRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLHQQYRLARQRIIQFQARCRAYLVRKAFRHRLWAVLTVQAYARGMIARRLHQRLRAEYLWRLEAEKMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEAARRKKELLEQMERARHEPVNHSDMVDKMFGFLGTSGGLPGQEGQAPSGFEDLERGRREMVEEDLDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGEAQLPEGQKKSSVRHKLVHLTLKKKSKLTEEVTKRLHDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTAKELCNALADKISLKDRFGFSLYIALFDKQRHAQQRGPRPQAQGRYRVAGHSLPGFFSPMLLCFQVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHSPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLNLVPTYIPDREITPLKTLEKWAQLAIAAHKKGIYAQRRTDAQKVKEDVVSYARFKWPLLFSRFYEAYKFSGPSLPKNDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRGAKTTAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDSVYVMPTVTMPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKLLGSEELSQEACLAFIAVLKYMGDYPSKRTRSVNELTDQIFEGPLKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIATRLLLKSSEGFSLFVKIADKVLSVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKVHHGLLRAEEEGNRALIKHRDSSLGLWERAMALDTCPGEGRRAAPSALSQLSVLAGVSPGDRGTRRSSAWQWRQTCKQAGVMEDGLCSDTGEPSPRWQSSGGSAGCEKAS

Target ORF information:

RefSeq Version	XM_017017779.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VIIA (MYO7A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017779.1

ORF Insert Sequence:

ATGGAGGACC CCGCCGACCC TGCCGACCCC GCGCGCATCC CGCAGCCCGG CAAGTGGGCG 
CTGGTGCGCA GCCTGAACCA GGCGTTCAAG ACCTACGCCG TCCTGCCGGG GGACCATGTG 
TGGATGGACC TGAGATTGGG GCAGGAGTTC GACGTGCCCA TCGGGGCGGT GGTGAAGCTC 
TGCGACTCTG GGCAGGTCCA GGTGGTGGAT GATGAAGACA ATGAACACTG GATCTCTCCG 
CAGAACGCAA CGCACATCAA GCCTATGCAC CCCACGTCGG TCCACGGCGT GGAGGACATG 
ATCCGCCTGG GGGACCTCAA CGAGGCGGGC ATCTTGCGCA ACCTGCTTAT CCGCTACCGG 
GACCACCTCA TCTACACGTA TACGGGCTCC ATCCTGGTGG CTGTGAACCC CTACCAGCTG 
CTCTCCATCT ACTCGCCAGA GCACATCCGC CAGTATACCA ACAAGAAGAT TGGGGAGATG 
CCCCCCCACA TCTTTGCCAT TGCTGACAAC TGCTACTTCA ACATGAAACG CAACAGCCGA 
GACCAGTGCT GCATCATCAG TGGGGAATCT GGGGCCGGGA AGACGGAGAG CACAAAGCTG 
ATCCTGCAGT TCCTGGCAGC CATCAGTGGG CAGCACTCGT GGATTGAGCA GCAGGTCTTG 
GAGGCCACCC CCATTCTGGA AGCATTTGGG AATGCCAAGA CCATCCGCAA TGACAACTCA 
AGCCGTTTCG GAAAGTACAT CGACATCCAC TTCAACAAGC GGGGCGCCAT CGAGGGCGCG 
AAGATTGAGC AGTACCTGCT GGAAAAGTCA CGTGTCTGTC GCCAGGCCCT GGATGAAAGG 
AACTACCACG TGTTCTACTG CATGCTGGAG GGTATGAGTG AGGATCAGAA GAAGAAGCTG 
GGCTTGGGCC AGGCCTCTGA CTACAACTAC TTGGCCATGG GTAACTGCAT AACCTGTGAG 
GGCCGGGTGG ACAGCCAGGA GTACGCCAAC ATCCGCTCCG CCATGAAGGT GCTCATGTTC 
ACTGACACCG AGAACTGGGA GATCTCGAAG CTCCTGGCTG CCATCCTGCA CCTGGGCAAC 
CTGCAGTATG AGGCACGCAC ATTTGAAAAC CTGGATGCCT GTGAGGTTCT CTTCTCCCCA 
TCGCTGGCCA CAGCTGCATC CCTGCTTGAG GTGAACCCCC CAGACCTGAT GAGCTGCCTG 
ACTAGCCGCA CCCTCATCAC CCGCGGGGAG ACGGTGTCCA CCCCACTGAG CAGGGAACAG 
GCACTGGACG TGCGCGACGC CTTCGTAAAG GGGATCTACG GGCGGCTGTT CGTGTGGATT 
GTGGACAAGA TCAACGCAGC AATTTACAAG CCTCCCTCCC AGGATGTGAA GAACTCTCGC 
AGGTCCATCG GCCTCCTGGA CATCTTTGGG TTTGAGAACT TTGCTGTGAA CAGCTTTGAG 
CAGCTCTGCA TCAACTTCGC CAATGAGCAC CTGCAGCAGT TCTTTGTGCG GCACGTGTTC 
AAGCTGGAGC AGGAGGAATA TGACCTGGAG AGCATTGACT GGCTGCACAT CGAGTTCACT 
GACAACCAGG ATGCCCTGGA CATGATTGCC AACAAGCCCA TGAACATCAT CTCCCTCATC 
GATGAGGAGA GCAAGTTCCC CAAGGGCACA GACACCACCA TGTTACACAA GCTGAACTCC 
CAGCACAAGC TCAACGCCAA CTACATCCCC CCCAAGAACA ACCATGAGAC CCAGTTTGGC 
ATCAACCATT TTGCAGGCAT CGTCTACTAT GAGACCCAAG GCTTCCTGGA GAAGAACCGA 
GACACCCTGC ATGGGGACAT TATCCAGCTG GTCCACTCCT CCAGGAACAA GTTCATCAAG 
CAGATCTTCC AGGCCGATGT CGCCATGGGC GCCGAGACCA GGAAGCGCTC GCCCACACTT 
AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG CTGATGCGCA CGCTGGGTGC CTGCCAGCCC 
TTCTTTGTGC GATGCATCAA GCCCAATGAG TTCAAGAAGC CCATGCTGTT CGACCGGCAC 
CTGTGCGTGC GCCAGCTGCG GTACTCAGGA ATGATGGAGA CCATCCGAAT CCGCCGAGCT 
GGCTACCCCA TCCGCTACAG CTTCGTAGAG TTTGTGGAGC GGTACCGTGT GCTGCTGCCA 
GGTGTGAAGC CGGCCTACAA GCAGGGCGAC CTCCGCGGGA CTTGCCAGCG CATGGCTGAG 
GCTGTGCTGG GCACCCACGA TGACTGGCAG ATAGGCAAAA CCAAGATCTT TCTGAAGGAC 
CACCATGACA TGCTGCTGGA AGTGGAGCGG GACAAAGCCA TCACCGACAG AGTCATCCTC 
CTTCAGAAAG TCATCCGGGG ATTCAAAGAC AGGTCTAACT TTCTGAAGCT GAAGAACGCT 
GCCACACTGA TCCAGAGGCA CTGGCGGGGT CACAACTGTA GGAAGAACTA CGGGCTGATG 
CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG CACCGCTCCC GGAAGCTGCA CCAGCAGTAC 
CGCCTGGCCC GCCAGCGCAT CATCCAGTTC CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC 
AAGGCCTTCC GCCACCGCCT CTGGGCTGTG CTCACCGTGC AGGCCTATGC CCGGGGCATG 
ATCGCCCGCA GGCTGCACCA ACGCCTCAGG GCTGAGTATC TGTGGCGCCT CGAGGCTGAG 
AAAATGCGGC TGGCGGAGGA AGAGAAGCTT CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG 
GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC CTGGCCCAGC TGGCTCGTGA GGACGCTGAG 
CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG CGGAAGAAGG AGCTCCTGGA GCAGATGGAA 
AGGGCCCGCC ATGAGCCTGT CAATCACTCA GACATGGTGG ACAAGATGTT TGGCTTCCTG 
GGGACTTCAG GTGGCCTGCC AGGCCAGGAG GGCCAGGCAC CTAGTGGCTT TGAGGACCTG 
GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG GACCTGGATG CAGCCCTGCC CCTGCCTGAC 
GAGGATGAGG AGGACCTCTC TGAGTATAAA TTTGCCAAGT TCGCGGCCAC CTACTTCCAG 
GGGACAACCA CGCACTCCTA CACCCGGCGG CCACTCAAAC AGCCACTGCT CTACCATGAC 
GACGAGGGTG ACCAGCTGGC AGCCCTGGCG GTCTGGATCA CCATCCTCCG CTTCATGGGG 
GACCTCCCTG AGCCCAAGTA CCACACAGCC ATGAGTGATG GCAGTGAGAA GATCCCTGTG 
ATGACCAAGA TTTATGAGAC CCTGGGCAAG AAGACGTACA AGAGGGAGCT GCAGGCCCTG 
CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG GGCCAGAAGA AGAGCAGTGT GAGGCACAAG 
CTGGTGCATT TGACTCTGAA AAAGAAGTCC AAGCTCACAG AGGAGGTGAC CAAGAGGCTG 
CATGACGGGG AGTCCACAGT GCAGGGCAAC AGCATGCTGG AGGACCGGCC CACCTCCAAC 
CTGGAGAAGC TGCACTTCAT CATCGGCAAT GGCATCCTGC GGCCAGCACT CCGGGACGAG 
ATCTACTGCC AGATCAGCAA GCAGCTGACC CACAACCCCT CCAAGAGCAG CTATGCCCGG 
GGCTGGATTC TCGTGTCTCT CTGCGTGGGC TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG 
TACCTGCGGA ACTTCATCCA CGGGGGCCCG CCCGGCTACG CCCCGTACTG TGAGGAGCGC 
CTGAGAAGGA CCTTTGTCAA TGGGACACGG ACACAGCCGC CCAGCTGGCT GGAGCTGCAG 
GCCACCAAGT CCAAGAAGCC AATCATGTTG CCCGTGACAT TCATGGATGG GACCACCAAG 
ACCCTGCTGA CGGACTCGGC AACCACGGCC AAGGAGCTCT GCAACGCGCT GGCCGACAAG 
ATCTCTCTCA AGGACCGGTT CGGGTTCTCC CTCTACATTG CCCTGTTTGA CAAGCAACGT 
CATGCTCAGC AGAGGGGCCC CCGCCCACAA GCCCAGGGGA GGTACCGTGT TGCTGGACAC 
AGTTTGCCTG GATTTTTCTC ACCGATGCTC TTATGTTTCC AGGTGTCCTC CCTGGGCAGC 
GGCAGTGACC ACGTCATGGA CGCCATCTCC CAGTGCGAGC AGTACGCCAA GGAGCAGGGC 
GCCCAGGAGC GCAACGCCCC CTGGAGGCTC TTCTTCCGCA AAGAGGTCTT CACGCCCTGG 
CACAGCCCCT CCGAGGACAA CGTGGCCACC AACCTCATCT ACCAGCAGGT GGTGCGAGGA 
GTCAAGTTTG GGGAGTACAG GTGTGAGAAG GAGGACGACC TGGCTGAGCT GGCCTCCCAG 
CAGTACTTTG TAGACTATGG CTCTGAGATG ATCCTGGAGC GCCTCCTGAA CCTCGTGCCC 
ACCTACATCC CCGACCGCGA GATCACGCCC CTGAAGACGC TGGAGAAGTG GGCCCAGCTG 
GCCATCGCCG CCCACAAGAA GGGGATTTAT GCCCAGAGGA GAACTGATGC CCAGAAGGTC 
AAAGAGGATG TGGTCAGTTA TGCCCGCTTC AAGTGGCCCT TGCTCTTCTC CAGGTTTTAT 
GAAGCCTACA AATTCTCAGG CCCCAGTCTC CCCAAGAACG ACGTCATCGT GGCCGTCAAC 
TGGACGGGTG TGTACTTTGT GGATGAGCAG GAGCAGGTAC TTCTGGAGCT GTCCTTCCCA 
GAGATCATGG CCGTGTCCAG CAGCAGGGGA GCGAAAACGA CGGCCCCCAG CTTCACGCTG 
GCCACCATCA AGGGGGACGA ATACACCTTC ACCTCCAGCA ATGCTGAGGA CATTCGTGAC 
CTGGTGGTCA CCTTCCTAGA GGGGCTCCGG AAGAGATCTA AGTATGTTGT GGCCCTGCAG 
GATAACCCCA ACCCCGGCGA GGAGTCAGGC TTCCTCAGCT TTGCCAAGGG AGACCTCATC 
ATCCTGGACC ATGACACGGG CGAGCAGGTC ATGAACTCGG GCTGGGCCAA CGGCATCAAT 
GAGAGGACCA AGCAGCGTGG GGACTTCCCC ACCGACAGTG TGTACGTCAT GCCCACTGTC 
ACCATGCCAC CGCGGGAGAT TGTGGCCCTG GTCACCATGA CTCCCGATCA GAGGCAGGAC 
GTTGTCCGGC TCTTGCAGCT GCGAACGGCG GAGCCCGAGG TGCGTGCCAA GCCCTACACG 
CTGGAGGAGT TTTCCTATGA CTACTTCAGG CCCCCACCCA AGCACACGCT GAGCCGTGTC 
ATGGTGTCCA AGGCCCGAGG CAAGGACCGG CTGTGGAGCC ACACGCGGGA ACCGCTCAAG 
CAGGCGCTGC TCAAGAAGCT CCTGGGCAGT GAGGAGCTCT CGCAGGAGGC CTGCCTGGCC 
TTCATTGCTG TGCTCAAGTA CATGGGCGAC TACCCGTCCA AGAGGACACG CTCCGTCAAC 
GAGCTCACCG ACCAGATCTT TGAGGGTCCC CTGAAAGCCG AGCCCCTGAA GGACGAGGCA 
TATGTGCAGA TCCTGAAGCA GCTGACCGAC AACCACATCA GGTACAGCGA GGAGCGGGGT 
TGGGAGCTGC TCTGGCTGTG CACGGGCCTT TTCCCACCCA GCAACATCCT CCTGCCCCAC 
GTGCAGCGCT TCCTGCAGTC CCGAAAGCAC TGCCCACTCG CCATCGACTG CCTGCAACGG 
CTCCAGAAAG CCCTGAGAAA CGGGTCCCGG AAGTACCCTC CGCACCTGGT GGAGGTGGAG 
GCCATCCAGC ACAAGACCAC CCAGATTTTC CACAAAGTCT ACTTCCCTGA TGACACTGAC 
GAGGCCTTCG AAGTGGAGTC CAGCACCAAG GCCAAGGACT TCTGCCAGAA CATCGCCACC 
AGGCTGCTCC TCAAGTCCTC AGAGGGATTC AGCCTCTTTG TCAAAATTGC AGACAAGGTC 
CTCAGCGTTC CTGAGAATGA CTTCTTCTTT GACTTTGTTC GACACTTGAC AGACTGGATA 
AAGAAAGCTC GGCCCATCAA GGACGGAATT GTGCCCTCAC TCACCTACCA GGTGTTCTTC 
ATGAAGAAGC TGTGGACCAC CACGGTGCCA GGGAAGGATC CCATGGCCGA TTCCATCTTC 
CACTATTACC AGGAGTTGCC CAAGTATCTC CGAGGCTACC ACAAGTGCAC GCGGGAGGAG 
GTGCTGCAGC TGGGGGCGCT GATCTACAGG GTCAAGTTCG AGGAGGACAA GTCCTACTTC 
CCCAGCATCC CCAAGCTGCT GCGGGAGCTG GTGCCCCAGG ACCTTATCCG GCAGGTCTCA 
CCTGATGACT GGAAGCGGTC CATCGTCGCC TACTTCAACA AGCACGCAGG GAAGTCCAAG 
GAGGAGGCCA AGCTGGCCTT CCTGAAGCTC ATCTTCAAGT GGCCCACCTT TGGCTCAGCC 
TTCTTCGAGG TGAAGGTACA CCATGGGCTT CTCAGAGCAG AGGAGGAGGG GAACAGGGCA 
TTGATAAAGC ACAGAGACTC CTCCCTAGGA CTGTGGGAAA GAGCCATGGC CTTGGACACC 
TGCCCTGGTG AGGGAAGGAG GGCAGCACCC TCAGCCTTGT CCCAGCTTAG CGTCTTAGCT 
GGGGTAAGTC CTGGGGACAG AGGGACCAGA AGAAGCTCAG CCTGGCAGTG GAGGCAGACA 
TGTAAACAGG CTGGGGTAAT GGAGGATGGC CTGTGTTCTG ACACTGGGGA GCCCTCGCCC 
AGGTGGCAGA GCTCGGGAGG CTCTGCCGGG TGTGAGAAGG CCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO7A ( XM_017017780.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873269.1 Homo sapiens myosin VIIA (MYO7A), transcript variant X4, mRNA.

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CloneID	OHu92257
Clone ID Related Accession (Same CDS sequence)	XM_017017780.1
Accession Version	XM_017017780.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6738bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	unconventional myosin-VIIa isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACC CCGCCGACCC TGCCGACCCC GCGCGCATCC CGCAGCCCGG CAAGTGGGCG 
CTGGTGCGCA GCCTGAACCA GGCGTTCAAG ACCTACGCCG TCCTGCCGGG GGACCATGTG 
TGGATGGACC TGAGATTGGG GCAGGAGTTC GACGTGCCCA TCGGGGCGGT GGTGAAGCTC 
TGCGACTCTG GGCAGGTCCA GGTGGTGGAT GATGAAGACA ATGAACACTG GATCTCTCCG 
CAGAACGCAA CGCACATCAA GCCTATGCAC CCCACGTCGG TCCACGGCGT GGAGGACATG 
ATCCGCCTGG GGGACCTCAA CGAGGCGGGC ATCTTGCGCA ACCTGCTTAT CCGCTACCGG 
GACCACCTCA TCTACACGTA TACGGGCTCC ATCCTGGTGG CTGTGAACCC CTACCAGCTG 
CTCTCCATCT ACTCGCCAGA GCACATCCGC CAGTATACCA ACAAGAAGAT TGGGGAGATG 
CCCCCCCACA TCTTTGCCAT TGCTGACAAC TGCTACTTCA ACATGAAACG CAACAGCCGA 
GACCAGTGCT GCATCATCAG TGGGGAATCT GGGGCCGGGA AGACGGAGAG CACAAAGCTG 
ATCCTGCAGT TCCTGGCAGC CATCAGTGGG CAGCACTCGT GGATTGAGCA GCAGGTCTTG 
GAGGCCACCC CCATTCTGGA AGCATTTGGG AATGCCAAGA CCATCCGCAA TGACAACTCA 
AGCCGTTTCG GAAAGTACAT CGACATCCAC TTCAACAAGC GGGGCGCCAT CGAGGGCGCG 
AAGATTGAGC AGTACCTGCT GGAAAAGTCA CGTGTCTGTC GCCAGGCCCT GGATGAAAGG 
AACTACCACG TGTTCTACTG CATGCTGGAG GGTATGAGTG AGGATCAGAA GAAGAAGCTG 
GGCTTGGGCC AGGCCTCTGA CTACAACTAC TTGGCCATGG GTAACTGCAT AACCTGTGAG 
GGCCGGGTGG ACAGCCAGGA GTACGCCAAC ATCCGCTCCG CCATGAAGGT GCTCATGTTC 
ACTGACACCG AGAACTGGGA GATCTCGAAG CTCCTGGCTG CCATCCTGCA CCTGGGCAAC 
CTGCAGTATG AGGCACGCAC ATTTGAAAAC CTGGATGCCT GTGAGGTTCT CTTCTCCCCA 
TCGCTGGCCA CAGCTGCATC CCTGCTTGAG GTGAACCCCC CAGACCTGAT GAGCTGCCTG 
ACTAGCCGCA CCCTCATCAC CCGCGGGGAG ACGGTGTCCA CCCCACTGAG CAGGGAACAG 
GCACTGGACG TGCGCGACGC CTTCGTAAAG GGGATCTACG GGCGGCTGTT CGTGTGGATT 
GTGGACAAGA TCAACGCAGC AATTTACAAG CCTCCCTCCC AGGATGTGAA GAACTCTCGC 
AGGTCCATCG GCCTCCTGGA CATCTTTGGG TTTGAGAACT TTGCTGTGAA CAGCTTTGAG 
CAGCTCTGCA TCAACTTCGC CAATGAGCAC CTGCAGCAGT TCTTTGTGCG GCACGTGTTC 
AAGCTGGAGC AGGAGGAATA TGACCTGGAG AGCATTGACT GGCTGCACAT CGAGTTCACT 
GACAACCAGG ATGCCCTGGA CATGATTGCC AACAAGCCCA TGAACATCAT CTCCCTCATC 
GATGAGGAGA GCAAGTTCCC CAAGGGCACA GACACCACCA TGTTACACAA GCTGAACTCC 
CAGCACAAGC TCAACGCCAA CTACATCCCC CCCAAGAACA ACCATGAGAC CCAGTTTGGC 
ATCAACCATT TTGCAGGCAT CGTCTACTAT GAGACCCAAG GCTTCCTGGA GAAGAACCGA 
GACACCCTGC ATGGGGACAT TATCCAGCTG GTCCACTCCT CCAGGAACAA GTTCATCAAG 
CAGATCTTCC AGGCCGATGT CGCCATGGGC GCCGAGACCA GGAAGCGCTC GCCCACACTT 
AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG CTGATGCGCA CGCTGGGTGC CTGCCAGCCC 
TTCTTTGTGC GATGCATCAA GCCCAATGAG TTCAAGAAGC CCATGCTGTT CGACCGGCAC 
CTGTGCGTGC GCCAGCTGCG GTACTCAGGA ATGATGGAGA CCATCCGAAT CCGCCGAGCT 
GGCTACCCCA TCCGCTACAG CTTCGTAGAG TTTGTGGAGC GGTACCGTGT GCTGCTGCCA 
GGTGTGAAGC CGGCCTACAA GCAGGGCGAC CTCCGCGGGA CTTGCCAGCG CATGGCTGAG 
GCTGTGCTGG GCACCCACGA TGACTGGCAG ATAGGCAAAA CCAAGATCTT TCTGAAGGAC 
CACCATGACA TGCTGCTGGA AGTGGAGCGG GACAAAGCCA TCACCGACAG AGTCATCCTC 
CTTCAGAAAG TCATCCGGGG ATTCAAAGAC AGGTCTAACT TTCTGAAGCT GAAGAACGCT 
GCCACACTGA TCCAGAGGCA CTGGCGGGGT CACAACTGTA GGAAGAACTA CGGGCTGATG 
CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG CACCGCTCCC GGAAGCTGCA CCAGCAGTAC 
CGCCTGGCCC GCCAGCGCAT CATCCAGTTC CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC 
AAGGCCTTCC GCCACCGCCT CTGGGCTGTG CTCACCGTGC AGGCCTATGC CCGGGGCATG 
ATCGCCCGCA GGCTGCACCA ACGCCTCAGG GCTGAGTATC TGTGGCGCCT CGAGGCTGAG 
AAAATGCGGC TGGCGGAGGA AGAGAAGCTT CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG 
GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC CTGGCCCAGC TGGCTCGTGA GGACGCTGAG 
CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG CGGAAGAAGG AGCTCCTGGA GCAGATGGAA 
AGGGCCCGCC ATGAGCCTGT CAATCACTCA GACATGGTGG ACAAGATGTT TGGCTTCCTG 
GGGACTTCAG GTGGCCTGCC AGGCCAGGAG GGCCAGGCAC CTAGTGGCTT TGAGGACCTG 
GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG GACCTGGATG CAGCCCTGCC CCTGCCTGAC 
GAGGATGAGG AGGACCTCTC TGAGTATAAA TTTGCCAAGT TCGCGGCCAC CTACTTCCAG 
GGGACAACCA CGCACTCCTA CACCCGGCGG CCACTCAAAC AGCCACTGCT CTACCATGAC 
GACGAGGGTG ACCAGCTGGC AGCCCTGGCG GTCTGGATCA CCATCCTCCG CTTCATGGGG 
GACCTCCCTG AGCCCAAGTA CCACACAGCC ATGAGTGATG GCAGTGAGAA GATCCCTGTG 
ATGACCAAGA TTTATGAGAC CCTGGGCAAG AAGACGTACA AGAGGGAGCT GCAGGCCCTG 
CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG GGCCAGAAGA AGAGCAGTGT GAGGCACAAG 
CTGGTGCATT TGACTCTGAA AAAGAAGTCC AAGCTCACAG AGGAGGTGAC CAAGAGGCTG 
CATGACGGGG AGTCCACAGT GCAGGGCAAC AGCATGCTGG AGGACCGGCC CACCTCCAAC 
CTGGAGAAGC TGCACTTCAT CATCGGCAAT GGCATCCTGC GGCCAGCACT CCGGGACGAG 
ATCTACTGCC AGATCAGCAA GCAGCTGACC CACAACCCCT CCAAGAGCAG CTATGCCCGG 
GGCTGGATTC TCGTGTCTCT CTGCGTGGGC TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG 
TACCTGCGGA ACTTCATCCA CGGGGGCCCG CCCGGCTACG CCCCGTACTG TGAGGAGCGC 
CTGAGAAGGA CCTTTGTCAA TGGGACACGG ACACAGCCGC CCAGCTGGCT GGAGCTGCAG 
GCCACCAAGT CCAAGAAGCC AATCATGTTG CCCGTGACAT TCATGGATGG GACCACCAAG 
ACCCTGCTGA CGGACTCGGC AACCACGGCC AAGGAGCTCT GCAACGCGCT GGCCGACAAG 
ATCTCTCTCA AGGACCGGTT CGGGTTCTCC CTCTACATTG CCCTGTTTGA CAAGGTGTCC 
TCCCTGGGCA GCGGCAGTGA CCACGTCATG GACGCCATCT CCCAGTGCGA GCAGTACGCC 
AAGGAGCAGG GCGCCCAGGA GCGCAACGCC CCCTGGAGGC TCTTCTTCCG CAAAGAGGTC 
TTCACGCCCT GGCACAGCCC CTCCGAGGAC AACGTGGCCA CCAACCTCAT CTACCAGCAG 
GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC AGGTGTGAGA AGGAGGACGA CCTGGCTGAG 
CTGGCCTCCC AGCAGTACTT TGTAGACTAT GGCTCTGAGA TGATCCTGGA GCGCCTCCTG 
AACCTCGTGC CCACCTACAT CCCCGACCGC GAGATCACGC CCCTGAAGAC GCTGGAGAAG 
TGGGCCCAGC TGGCCATCGC CGCCCACAAG AAGGGGATTT ATGCCCAGAG GAGAACTGAT 
GCCCAGAAGG TCAAAGAGGA TGTGGTCAGT TATGCCCGCT TCAAGTGGCC CTTGCTCTTC 
TCCAGGTTTT ATGAAGCCTA CAAATTCTCA GGCCCCAGTC TCCCCAAGAA CGACGTCATC 
GTGGCCGTCA ACTGGACGGG TGTGTACTTT GTGGATGAGC AGGAGCAGGT ACTTCTGGAG 
CTGTCCTTCC CAGAGATCAT GGCCGTGTCC AGCAGCAGGG AGTGCCGTGT CTGGCTCTCA 
CTGGGCTGCT CTGATCTTGG CTGTGCTGCG CCTCACTCAG GCTGGGCAGG ACTGACCCCG 
GCGGGGCCCT GTTCTCCGTG TTGGTCCTGC AGGGGAGCGA AAACGACGGC CCCCAGCTTC 
ACGCTGGCCA CCATCAAGGG GGACGAATAC ACCTTCACCT CCAGCAATGC TGAGGACATT 
CGTGACCTGG TGGTCACCTT CCTAGAGGGG CTCCGGAAGA GATCTAAGTA TGTTGTGGCC 
CTGCAGGATA ACCCCAACCC CGCAGGCGAG GAGTCAGGCT TCCTCAGCTT TGCCAAGGGA 
GACCTCATCA TCCTGGACCA TGACACGGGC GAGCAGGTCA TGAACTCGGG CTGGGCCAAC 
GGCATCAATG AGAGGACCAA GCAGCGTGGG GACTTCCCCA CCGACAGTGT GTACGTCATG 
CCCACTGTCA CCATGCCACC GCGGGAGATT GTGGCCCTGG TCACCATGAC TCCCGATCAG 
AGGCAGGACG TTGTCCGGCT CTTGCAGCTG CGAACGGCGG AGCCCGAGGT GCGTGCCAAG 
CCCTACACGC TGGAGGAGTT TTCCTATGAC TACTTCAGGC CCCCACCCAA GCACACGCTG 
AGCCGTGTCA TGGTGTCCAA GGCCCGAGGC AAGGACCGGC TGTGGAGCCA CACGCGGGAA 
CCGCTCAAGC AGGCGCTGCT CAAGAAGCTC CTGGGCAGTG AGGAGCTCTC GCAGGAGGCC 
TGCCTGGCCT TCATTGCTGT GCTCAAGTAC ATGGGCGACT ACCCGTCCAA GAGGACACGC 
TCCGTCAACG AGCTCACCGA CCAGATCTTT GAGGGTCCCC TGAAAGCCGA GCCCCTGAAG 
GACGAGGCAT ATGTGCAGAT CCTGAAGCAG CTGACCGACA ACCACATCAG GTACAGCGAG 
GAGCGGGGTT GGGAGCTGCT CTGGCTGTGC ACGGGCCTTT TCCCACCCAG CAACATCCTC 
CTGCCCCACG TGCAGCGCTT CCTGCAGTCC CGAAAGCACT GCCCACTCGC CATCGACTGC 
CTGCAACGGC TCCAGAAAGC CCTGAGAAAC GGGTCCCGGA AGTACCCTCC GCACCTGGTG 
GAGGTGGAGG CCATCCAGCA CAAGACCACC CAGATTTTCC ACAAAGTCTA CTTCCCTGAT 
GACACTGACG AGGCCTTCGA AGTGGAGTCC AGCACCAAGG CCAAGGACTT CTGCCAGAAC 
ATCGCCACCA GGCTGCTCCT CAAGTCCTCA GAGGGATTCA GCCTCTTTGT CAAAATTGCA 
GACAAGGTCC TCAGCGTTCC TGAGAATGAC TTCTTCTTTG ACTTTGTTCG ACACTTGACA 
GACTGGATAA AGAAAGCTCG GCCCATCAAG GACGGAATTG TGCCCTCACT CACCTACCAG 
GTGTTCTTCA TGAAGAAGCT GTGGACCACC ACGGTGCCAG GGAAGGATCC CATGGCCGAT 
TCCATCTTCC ACTATTACCA GGAGTTGCCC AAGTATCTCC GAGGCTACCA CAAGTGCACG 
CGGGAGGAGG TGCTGCAGCT GGGGGCGCTG ATCTACAGGG TCAAGTTCGA GGAGGACAAG 
TCCTACTTCC CCAGCATCCC CAAGCTGCTG CGGGAGCTGG TGCCCCAGGA CCTTATCCGG 
CAGGTCTCAC CTGATGACTG GAAGCGGTCC ATCGTCGCCT ACTTCAACAA GCACGCAGGG 
AAGTCCAAGG AGGAGGCCAA GCTGGCCTTC CTGAAGCTCA TCTTCAAGTG GCCCACCTTT 
GGCTCAGCCT TCTTCGAGGT GAAGCAAACT ACGGAGCCAA ACTTCCCTGA GATCCTCCTA 
ATTGCCATCA ACAAGTATGG GGTCAGCCTC ATCGATCCCA AAACGAAGGA TATCCTCACC 
ACTCATCCCT TCACCAAGAT CTCCAACTGG AGCAGCGGCA ACACCTACTT CCACATCACC 
ATTGGGAACT TGGTGCGCGG GAGCAAACTG CTCTGCGAGA CGTCACTGGG CTACAAGATG 
GATGACCTCC TGACTTCCTA CATTAGCCAG ATGCTCACAG CCATGAGCAA ACAGCGGGGC 
TCCAGGAGCG GCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873269.1
CDS	16..6753
Translation	MEDPADPADPARIPQPGKWALVRSLNQAFKTYAVLPGDHVWMDLRLGQEFDVPIGAVVKLCDSGQVQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNSRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQALDERNYHVFYCMLEGMSEDQKKKLGLGQASDYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHLGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVDKINAAIYKPPSQDVKNSRRSIGLLDIFGFENFAVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQDALDMIANKPMNIISLIDEESKFPKGTDTTMLHKLNSQHKLNANYIPPKNNHETQFGINHFAGIVYYETQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRRAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLKLKNAATLIQRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLHQQYRLARQRIIQFQARCRAYLVRKAFRHRLWAVLTVQAYARGMIARRLHQRLRAEYLWRLEAEKMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEAARRKKELLEQMERARHEPVNHSDMVDKMFGFLGTSGGLPGQEGQAPSGFEDLERGRREMVEEDLDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGEAQLPEGQKKSSVRHKLVHLTLKKKSKLTEEVTKRLHDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTAKELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHSPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLNLVPTYIPDREITPLKTLEKWAQLAIAAHKKGIYAQRRTDAQKVKEDVVSYARFKWPLLFSRFYEAYKFSGPSLPKNDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRECRVWLSLGCSDLGCAAPHSGWAGLTPAGPCSPCWSCRGAKTTAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDSVYVMPTVTMPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKLLGSEELSQEACLAFIAVLKYMGDYPSKRTRSVNELTDQIFEGPLKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIATRLLLKSSEGFSLFVKIADKVLSVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKQTTEPNFPEILLIAINKYGVSLIDPKTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRGSRSGK

Target ORF information:

RefSeq Version	XM_017017780.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VIIA (MYO7A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017780.1

ORF Insert Sequence:

ATGGAGGACC CCGCCGACCC TGCCGACCCC GCGCGCATCC CGCAGCCCGG CAAGTGGGCG 
CTGGTGCGCA GCCTGAACCA GGCGTTCAAG ACCTACGCCG TCCTGCCGGG GGACCATGTG 
TGGATGGACC TGAGATTGGG GCAGGAGTTC GACGTGCCCA TCGGGGCGGT GGTGAAGCTC 
TGCGACTCTG GGCAGGTCCA GGTGGTGGAT GATGAAGACA ATGAACACTG GATCTCTCCG 
CAGAACGCAA CGCACATCAA GCCTATGCAC CCCACGTCGG TCCACGGCGT GGAGGACATG 
ATCCGCCTGG GGGACCTCAA CGAGGCGGGC ATCTTGCGCA ACCTGCTTAT CCGCTACCGG 
GACCACCTCA TCTACACGTA TACGGGCTCC ATCCTGGTGG CTGTGAACCC CTACCAGCTG 
CTCTCCATCT ACTCGCCAGA GCACATCCGC CAGTATACCA ACAAGAAGAT TGGGGAGATG 
CCCCCCCACA TCTTTGCCAT TGCTGACAAC TGCTACTTCA ACATGAAACG CAACAGCCGA 
GACCAGTGCT GCATCATCAG TGGGGAATCT GGGGCCGGGA AGACGGAGAG CACAAAGCTG 
ATCCTGCAGT TCCTGGCAGC CATCAGTGGG CAGCACTCGT GGATTGAGCA GCAGGTCTTG 
GAGGCCACCC CCATTCTGGA AGCATTTGGG AATGCCAAGA CCATCCGCAA TGACAACTCA 
AGCCGTTTCG GAAAGTACAT CGACATCCAC TTCAACAAGC GGGGCGCCAT CGAGGGCGCG 
AAGATTGAGC AGTACCTGCT GGAAAAGTCA CGTGTCTGTC GCCAGGCCCT GGATGAAAGG 
AACTACCACG TGTTCTACTG CATGCTGGAG GGTATGAGTG AGGATCAGAA GAAGAAGCTG 
GGCTTGGGCC AGGCCTCTGA CTACAACTAC TTGGCCATGG GTAACTGCAT AACCTGTGAG 
GGCCGGGTGG ACAGCCAGGA GTACGCCAAC ATCCGCTCCG CCATGAAGGT GCTCATGTTC 
ACTGACACCG AGAACTGGGA GATCTCGAAG CTCCTGGCTG CCATCCTGCA CCTGGGCAAC 
CTGCAGTATG AGGCACGCAC ATTTGAAAAC CTGGATGCCT GTGAGGTTCT CTTCTCCCCA 
TCGCTGGCCA CAGCTGCATC CCTGCTTGAG GTGAACCCCC CAGACCTGAT GAGCTGCCTG 
ACTAGCCGCA CCCTCATCAC CCGCGGGGAG ACGGTGTCCA CCCCACTGAG CAGGGAACAG 
GCACTGGACG TGCGCGACGC CTTCGTAAAG GGGATCTACG GGCGGCTGTT CGTGTGGATT 
GTGGACAAGA TCAACGCAGC AATTTACAAG CCTCCCTCCC AGGATGTGAA GAACTCTCGC 
AGGTCCATCG GCCTCCTGGA CATCTTTGGG TTTGAGAACT TTGCTGTGAA CAGCTTTGAG 
CAGCTCTGCA TCAACTTCGC CAATGAGCAC CTGCAGCAGT TCTTTGTGCG GCACGTGTTC 
AAGCTGGAGC AGGAGGAATA TGACCTGGAG AGCATTGACT GGCTGCACAT CGAGTTCACT 
GACAACCAGG ATGCCCTGGA CATGATTGCC AACAAGCCCA TGAACATCAT CTCCCTCATC 
GATGAGGAGA GCAAGTTCCC CAAGGGCACA GACACCACCA TGTTACACAA GCTGAACTCC 
CAGCACAAGC TCAACGCCAA CTACATCCCC CCCAAGAACA ACCATGAGAC CCAGTTTGGC 
ATCAACCATT TTGCAGGCAT CGTCTACTAT GAGACCCAAG GCTTCCTGGA GAAGAACCGA 
GACACCCTGC ATGGGGACAT TATCCAGCTG GTCCACTCCT CCAGGAACAA GTTCATCAAG 
CAGATCTTCC AGGCCGATGT CGCCATGGGC GCCGAGACCA GGAAGCGCTC GCCCACACTT 
AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG CTGATGCGCA CGCTGGGTGC CTGCCAGCCC 
TTCTTTGTGC GATGCATCAA GCCCAATGAG TTCAAGAAGC CCATGCTGTT CGACCGGCAC 
CTGTGCGTGC GCCAGCTGCG GTACTCAGGA ATGATGGAGA CCATCCGAAT CCGCCGAGCT 
GGCTACCCCA TCCGCTACAG CTTCGTAGAG TTTGTGGAGC GGTACCGTGT GCTGCTGCCA 
GGTGTGAAGC CGGCCTACAA GCAGGGCGAC CTCCGCGGGA CTTGCCAGCG CATGGCTGAG 
GCTGTGCTGG GCACCCACGA TGACTGGCAG ATAGGCAAAA CCAAGATCTT TCTGAAGGAC 
CACCATGACA TGCTGCTGGA AGTGGAGCGG GACAAAGCCA TCACCGACAG AGTCATCCTC 
CTTCAGAAAG TCATCCGGGG ATTCAAAGAC AGGTCTAACT TTCTGAAGCT GAAGAACGCT 
GCCACACTGA TCCAGAGGCA CTGGCGGGGT CACAACTGTA GGAAGAACTA CGGGCTGATG 
CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG CACCGCTCCC GGAAGCTGCA CCAGCAGTAC 
CGCCTGGCCC GCCAGCGCAT CATCCAGTTC CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC 
AAGGCCTTCC GCCACCGCCT CTGGGCTGTG CTCACCGTGC AGGCCTATGC CCGGGGCATG 
ATCGCCCGCA GGCTGCACCA ACGCCTCAGG GCTGAGTATC TGTGGCGCCT CGAGGCTGAG 
AAAATGCGGC TGGCGGAGGA AGAGAAGCTT CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG 
GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC CTGGCCCAGC TGGCTCGTGA GGACGCTGAG 
CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG CGGAAGAAGG AGCTCCTGGA GCAGATGGAA 
AGGGCCCGCC ATGAGCCTGT CAATCACTCA GACATGGTGG ACAAGATGTT TGGCTTCCTG 
GGGACTTCAG GTGGCCTGCC AGGCCAGGAG GGCCAGGCAC CTAGTGGCTT TGAGGACCTG 
GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG GACCTGGATG CAGCCCTGCC CCTGCCTGAC 
GAGGATGAGG AGGACCTCTC TGAGTATAAA TTTGCCAAGT TCGCGGCCAC CTACTTCCAG 
GGGACAACCA CGCACTCCTA CACCCGGCGG CCACTCAAAC AGCCACTGCT CTACCATGAC 
GACGAGGGTG ACCAGCTGGC AGCCCTGGCG GTCTGGATCA CCATCCTCCG CTTCATGGGG 
GACCTCCCTG AGCCCAAGTA CCACACAGCC ATGAGTGATG GCAGTGAGAA GATCCCTGTG 
ATGACCAAGA TTTATGAGAC CCTGGGCAAG AAGACGTACA AGAGGGAGCT GCAGGCCCTG 
CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG GGCCAGAAGA AGAGCAGTGT GAGGCACAAG 
CTGGTGCATT TGACTCTGAA AAAGAAGTCC AAGCTCACAG AGGAGGTGAC CAAGAGGCTG 
CATGACGGGG AGTCCACAGT GCAGGGCAAC AGCATGCTGG AGGACCGGCC CACCTCCAAC 
CTGGAGAAGC TGCACTTCAT CATCGGCAAT GGCATCCTGC GGCCAGCACT CCGGGACGAG 
ATCTACTGCC AGATCAGCAA GCAGCTGACC CACAACCCCT CCAAGAGCAG CTATGCCCGG 
GGCTGGATTC TCGTGTCTCT CTGCGTGGGC TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG 
TACCTGCGGA ACTTCATCCA CGGGGGCCCG CCCGGCTACG CCCCGTACTG TGAGGAGCGC 
CTGAGAAGGA CCTTTGTCAA TGGGACACGG ACACAGCCGC CCAGCTGGCT GGAGCTGCAG 
GCCACCAAGT CCAAGAAGCC AATCATGTTG CCCGTGACAT TCATGGATGG GACCACCAAG 
ACCCTGCTGA CGGACTCGGC AACCACGGCC AAGGAGCTCT GCAACGCGCT GGCCGACAAG 
ATCTCTCTCA AGGACCGGTT CGGGTTCTCC CTCTACATTG CCCTGTTTGA CAAGGTGTCC 
TCCCTGGGCA GCGGCAGTGA CCACGTCATG GACGCCATCT CCCAGTGCGA GCAGTACGCC 
AAGGAGCAGG GCGCCCAGGA GCGCAACGCC CCCTGGAGGC TCTTCTTCCG CAAAGAGGTC 
TTCACGCCCT GGCACAGCCC CTCCGAGGAC AACGTGGCCA CCAACCTCAT CTACCAGCAG 
GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC AGGTGTGAGA AGGAGGACGA CCTGGCTGAG 
CTGGCCTCCC AGCAGTACTT TGTAGACTAT GGCTCTGAGA TGATCCTGGA GCGCCTCCTG 
AACCTCGTGC CCACCTACAT CCCCGACCGC GAGATCACGC CCCTGAAGAC GCTGGAGAAG 
TGGGCCCAGC TGGCCATCGC CGCCCACAAG AAGGGGATTT ATGCCCAGAG GAGAACTGAT 
GCCCAGAAGG TCAAAGAGGA TGTGGTCAGT TATGCCCGCT TCAAGTGGCC CTTGCTCTTC 
TCCAGGTTTT ATGAAGCCTA CAAATTCTCA GGCCCCAGTC TCCCCAAGAA CGACGTCATC 
GTGGCCGTCA ACTGGACGGG TGTGTACTTT GTGGATGAGC AGGAGCAGGT ACTTCTGGAG 
CTGTCCTTCC CAGAGATCAT GGCCGTGTCC AGCAGCAGGG AGTGCCGTGT CTGGCTCTCA 
CTGGGCTGCT CTGATCTTGG CTGTGCTGCG CCTCACTCAG GCTGGGCAGG ACTGACCCCG 
GCGGGGCCCT GTTCTCCGTG TTGGTCCTGC AGGGGAGCGA AAACGACGGC CCCCAGCTTC 
ACGCTGGCCA CCATCAAGGG GGACGAATAC ACCTTCACCT CCAGCAATGC TGAGGACATT 
CGTGACCTGG TGGTCACCTT CCTAGAGGGG CTCCGGAAGA GATCTAAGTA TGTTGTGGCC 
CTGCAGGATA ACCCCAACCC CGCAGGCGAG GAGTCAGGCT TCCTCAGCTT TGCCAAGGGA 
GACCTCATCA TCCTGGACCA TGACACGGGC GAGCAGGTCA TGAACTCGGG CTGGGCCAAC 
GGCATCAATG AGAGGACCAA GCAGCGTGGG GACTTCCCCA CCGACAGTGT GTACGTCATG 
CCCACTGTCA CCATGCCACC GCGGGAGATT GTGGCCCTGG TCACCATGAC TCCCGATCAG 
AGGCAGGACG TTGTCCGGCT CTTGCAGCTG CGAACGGCGG AGCCCGAGGT GCGTGCCAAG 
CCCTACACGC TGGAGGAGTT TTCCTATGAC TACTTCAGGC CCCCACCCAA GCACACGCTG 
AGCCGTGTCA TGGTGTCCAA GGCCCGAGGC AAGGACCGGC TGTGGAGCCA CACGCGGGAA 
CCGCTCAAGC AGGCGCTGCT CAAGAAGCTC CTGGGCAGTG AGGAGCTCTC GCAGGAGGCC 
TGCCTGGCCT TCATTGCTGT GCTCAAGTAC ATGGGCGACT ACCCGTCCAA GAGGACACGC 
TCCGTCAACG AGCTCACCGA CCAGATCTTT GAGGGTCCCC TGAAAGCCGA GCCCCTGAAG 
GACGAGGCAT ATGTGCAGAT CCTGAAGCAG CTGACCGACA ACCACATCAG GTACAGCGAG 
GAGCGGGGTT GGGAGCTGCT CTGGCTGTGC ACGGGCCTTT TCCCACCCAG CAACATCCTC 
CTGCCCCACG TGCAGCGCTT CCTGCAGTCC CGAAAGCACT GCCCACTCGC CATCGACTGC 
CTGCAACGGC TCCAGAAAGC CCTGAGAAAC GGGTCCCGGA AGTACCCTCC GCACCTGGTG 
GAGGTGGAGG CCATCCAGCA CAAGACCACC CAGATTTTCC ACAAAGTCTA CTTCCCTGAT 
GACACTGACG AGGCCTTCGA AGTGGAGTCC AGCACCAAGG CCAAGGACTT CTGCCAGAAC 
ATCGCCACCA GGCTGCTCCT CAAGTCCTCA GAGGGATTCA GCCTCTTTGT CAAAATTGCA 
GACAAGGTCC TCAGCGTTCC TGAGAATGAC TTCTTCTTTG ACTTTGTTCG ACACTTGACA 
GACTGGATAA AGAAAGCTCG GCCCATCAAG GACGGAATTG TGCCCTCACT CACCTACCAG 
GTGTTCTTCA TGAAGAAGCT GTGGACCACC ACGGTGCCAG GGAAGGATCC CATGGCCGAT 
TCCATCTTCC ACTATTACCA GGAGTTGCCC AAGTATCTCC GAGGCTACCA CAAGTGCACG 
CGGGAGGAGG TGCTGCAGCT GGGGGCGCTG ATCTACAGGG TCAAGTTCGA GGAGGACAAG 
TCCTACTTCC CCAGCATCCC CAAGCTGCTG CGGGAGCTGG TGCCCCAGGA CCTTATCCGG 
CAGGTCTCAC CTGATGACTG GAAGCGGTCC ATCGTCGCCT ACTTCAACAA GCACGCAGGG 
AAGTCCAAGG AGGAGGCCAA GCTGGCCTTC CTGAAGCTCA TCTTCAAGTG GCCCACCTTT 
GGCTCAGCCT TCTTCGAGGT GAAGCAAACT ACGGAGCCAA ACTTCCCTGA GATCCTCCTA 
ATTGCCATCA ACAAGTATGG GGTCAGCCTC ATCGATCCCA AAACGAAGGA TATCCTCACC 
ACTCATCCCT TCACCAAGAT CTCCAACTGG AGCAGCGGCA ACACCTACTT CCACATCACC 
ATTGGGAACT TGGTGCGCGG GAGCAAACTG CTCTGCGAGA CGTCACTGGG CTACAAGATG 
GATGACCTCC TGACTTCCTA CATTAGCCAG ATGCTCACAG CCATGAGCAA ACAGCGGGGC 
TCCAGGAGCG GCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO7A ( XM_017017781.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873270.1 Homo sapiens myosin VIIA (MYO7A), transcript variant X6, mRNA.

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CloneID	OHu92258
Clone ID Related Accession (Same CDS sequence)	XM_017017781.1
Accession Version	XM_017017781.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	unconventional myosin-VIIa isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACC CCGCCGACCC TGCCGACCCC GCGCGCATCC CGCAGCCCGG CAAGTGGGCG 
CTGGTGCGCA GCCTGAACCA GGCGTTCAAG ACCTACGCCG TCCTGCCGGG GGACCATGTG 
TGGATGGACC TGAGATTGGG GCAGGAGTTC GACGTGCCCA TCGGGGCGGT GGTGAAGCTC 
TGCGACTCTG GGCAGGTCCA GGTGGTGGAT GATGAAGACA ATGAACACTG GATCTCTCCG 
CAGAACGCAA CGCACATCAA GCCTATGCAC CCCACGTCGG TCCACGGCGT GGAGGACATG 
ATCCGCCTGG GGGACCTCAA CGAGGCGGGC ATCTTGCGCA ACCTGCTTAT CCGCTACCGG 
GACCACCTCA TCTACACGTA TACGGGCTCC ATCCTGGTGG CTGTGAACCC CTACCAGCTG 
CTCTCCATCT ACTCGCCAGA GCACATCCGC CAGTATACCA ACAAGAAGAT TGGGGAGATG 
CCCCCCCACA TCTTTGCCAT TGCTGACAAC TGCTACTTCA ACATGAAACG CAACAGCCGA 
GACCAGTGCT GCATCATCAG TGGGGAATCT GGGGCCGGGA AGACGGAGAG CACAAAGCTG 
ATCCTGCAGT TCCTGGCAGC CATCAGTGGG CAGCACTCGT GGATTGAGCA GCAGGTCTTG 
GAGGCCACCC CCATTCTGGA AGCATTTGGG AATGCCAAGA CCATCCGCAA TGACAACTCA 
AGCCGTTTCG GAAAGTACAT CGACATCCAC TTCAACAAGC GGGGCGCCAT CGAGGGCGCG 
AAGATTGAGC AGTACCTGCT GGAAAAGTCA CGTGTCTGTC GCCAGGCCCT GGATGAAAGG 
AACTACCACG TGTTCTACTG CATGCTGGAG GGTATGAGTG AGGATCAGAA GAAGAAGCTG 
GGCTTGGGCC AGGCCTCTGA CTACAACTAC TTGGCCATGG GTAACTGCAT AACCTGTGAG 
GGCCGGGTGG ACAGCCAGGA GTACGCCAAC ATCCGCTCCG CCATGAAGGT GCTCATGTTC 
ACTGACACCG AGAACTGGGA GATCTCGAAG CTCCTGGCTG CCATCCTGCA CCTGGGCAAC 
CTGCAGTATG AGGCACGCAC ATTTGAAAAC CTGGATGCCT GTGAGGTTCT CTTCTCCCCA 
TCGCTGGCCA CAGCTGCATC CCTGCTTGAG GTGAACCCCC CAGACCTGAT GAGCTGCCTG 
ACTAGCCGCA CCCTCATCAC CCGCGGGGAG ACGGTGTCCA CCCCACTGAG CAGGGAACAG 
GCACTGGACG TGCGCGACGC CTTCGTAAAG GGGATCTACG GGCGGCTGTT CGTGTGGATT 
GTGGACAAGA TCAACGCAGC AATTTACAAG CCTCCCTCCC AGGATGTGAA GAACTCTCGC 
AGGTCCATCG GCCTCCTGGA CATCTTTGGG TTTGAGAACT TTGCTGTGAA CAGCTTTGAG 
CAGCTCTGCA TCAACTTCGC CAATGAGCAC CTGCAGCAGT TCTTTGTGCG GCACGTGTTC 
AAGCTGGAGC AGGAGGAATA TGACCTGGAG AGCATTGACT GGCTGCACAT CGAGTTCACT 
GACAACCAGG ATGCCCTGGA CATGATTGCC AACAAGCCCA TGAACATCAT CTCCCTCATC 
GATGAGGAGA GCAAGTTCCC CAAGGGCACA GACACCACCA TGTTACACAA GCTGAACTCC 
CAGCACAAGC TCAACGCCAA CTACATCCCC CCCAAGAACA ACCATGAGAC CCAGTTTGGC 
ATCAACCATT TTGCAGGCAT CGTCTACTAT GAGACCCAAG GCTTCCTGGA GAAGAACCGA 
GACACCCTGC ATGGGGACAT TATCCAGCTG GTCCACTCCT CCAGGAACAA GTTCATCAAG 
CAGATCTTCC AGGCCGATGT CGCCATGGGC GCCGAGACCA GGAAGCGCTC GCCCACACTT 
AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG CTGATGCGCA CGCTGGGTGC CTGCCAGCCC 
TTCTTTGTGC GATGCATCAA GCCCAATGAG TTCAAGAAGC CCATGCTGTT CGACCGGCAC 
CTGTGCGTGC GCCAGCTGCG GTACTCAGGA ATGATGGAGA CCATCCGAAT CCGCCGAGCT 
GGCTACCCCA TCCGCTACAG CTTCGTAGAG TTTGTGGAGC GGTACCGTGT GCTGCTGCCA 
GGTGTGAAGC CGGCCTACAA GCAGGGCGAC CTCCGCGGGA CTTGCCAGCG CATGGCTGAG 
GCTGTGCTGG GCACCCACGA TGACTGGCAG ATAGGCAAAA CCAAGATCTT TCTGAAGGAC 
CACCATGACA TGCTGCTGGA AGTGGAGCGG GACAAAGCCA TCACCGACAG AGTCATCCTC 
CTTCAGAAAG TCATCCGGGG ATTCAAAGAC AGGTCTAACT TTCTGAAGCT GAAGAACGCT 
GCCACACTGA TCCAGAGGCA CTGGCGGGGT CACAACTGTA GGAAGAACTA CGGGCTGATG 
CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG CACCGCTCCC GGAAGCTGCA CCAGCAGTAC 
CGCCTGGCCC GCCAGCGCAT CATCCAGTTC CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC 
AAGGCCTTCC GCCACCGCCT CTGGGCTGTG CTCACCGTGC AGGCCTATGC CCGGGGCATG 
ATCGCCCGCA GGCTGCACCA ACGCCTCAGG GCTGAGTATC TGTGGCGCCT CGAGGCTGAG 
AAAATGCGGC TGGCGGAGGA AGAGAAGCTT CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG 
GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC CTGGCCCAGC TGGCTCGTGA GGACGCTGAG 
CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG CGGAAGAAGG AGCTCCTGGA GCAGATGGAA 
AGGGCCCGCC ATGAGCCTGT CAATCACTCA GACATGGTGG ACAAGATGTT TGGCTTCCTG 
GGGACTTCAG GTGGCCTGCC AGGCCAGGAG GGCCAGGCAC CTAGTGGCTT TGAGGACCTG 
GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG GACCTGGATG CAGCCCTGCC CCTGCCTGAC 
GAGGATGAGG AGGACCTCTC TGAGTATAAA TTTGCCAAGT TCGCGGCCAC CTACTTCCAG 
GGGACAACCA CGCACTCCTA CACCCGGCGG CCACTCAAAC AGCCACTGCT CTACCATGAC 
GACGAGGGTG ACCAGCTGGC AGCCCTGGCG GTCTGGATCA CCATCCTCCG CTTCATGGGG 
GACCTCCCTG AGCCCAAGTA CCACACAGCC ATGAGTGATG GCAGTGAGAA GATCCCTGTG 
ATGACCAAGA TTTATGAGAC CCTGGGCAAG AAGACGTACA AGAGGGAGCT GCAGGCCCTG 
CAGGGCGAGG GCGAGGTGAC CAAGAGGCTG CATGACGGGG AGTCCACAGT GCAGGGCAAC 
AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATCGGCAAT 
GGCATCCTGC GGCCAGCACT CCGGGACGAG ATCTACTGCC AGATCAGCAA GCAGCTGACC 
CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATTC TCGTGTCTCT CTGCGTGGGC 
TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG TACCTGCGGA ACTTCATCCA CGGGGGCCCG 
CCCGGCTACG CCCCGTACTG TGAGGAGCGC CTGAGAAGGA CCTTTGTCAA TGGGACACGG 
ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC AATCATGTTG 
CCCGTGACAT TCATGGATGG GACCACCAAG ACCCTGCTGA CGGACTCGGC AACCACGGCC 
AAGGAGCTCT GCAACGCGCT GGCCGACAAG ATCTCTCTCA AGGACCGGTT CGGGTTCTCC 
CTCTACATTG CCCTGTTTGA CAAGCAACGT CATGCTCAGC AGAGGGGCCC CCGCCCACAA 
GCCCAGGGGA GGTACCGTGT TGCTGGACAC AGTTTGCCTG GATTTTTCTC ACCGATGCTC 
TTATGTTTCC AGGTGTCCTC CCTGGGCAGC GGCAGTGACC ACGTCATGGA CGCCATCTCC 
CAGTGCGAGC AGTACGCCAA GGAGCAGGGC GCCCAGGAGC GCAACGCCCC CTGGAGGCTC 
TTCTTCCGCA AAGAGGTCTT CACGCCCTGG CACAGCCCCT CCGAGGACAA CGTGGCCACC 
AACCTCATCT ACCAGCAGGT GGTGCGAGGA GTCAAGTTTG GGGAGTACAG GTGTGAGAAG 
GAGGACGACC TGGCTGAGCT GGCCTCCCAG CAGTACTTTG TAGACTATGG CTCTGAGATG 
ATCCTGGAGC GCCTCCTGAA CCTCGTGCCC ACCTACATCC CCGACCGCGA GATCACGCCC 
CTGAAGACGC TGGAGAAGTG GGCCCAGCTG GCCATCGCCG CCCACAAGAA GGGGATTTAT 
GCCCAGAGGA GAACTGATGC CCAGAAGGTC AAAGAGGATG TGGTCAGTTA TGCCCGCTTC 
AAGTGGCCCT TGCTCTTCTC CAGGTTTTAT GAAGCCTACA AATTCTCAGG CCCCAGTCTC 
CCCAAGAACG ACGTCATCGT GGCCGTCAAC TGGACGGGTG TGTACTTTGT GGATGAGCAG 
GAGCAGGTAC TTCTGGAGCT GTCCTTCCCA GAGATCATGG CCGTGTCCAG CAGCAGGGGA 
GCGAAAACGA CGGCCCCCAG CTTCACGCTG GCCACCATCA AGGGGGACGA ATACACCTTC 
ACCTCCAGCA ATGCTGAGGA CATTCGTGAC CTGGTGGTCA CCTTCCTAGA GGGGCTCCGG 
AAGAGATCTA AGTATGTTGT GGCCCTGCAG GATAACCCCA ACCCCGCAGG CGAGGAGTCA 
GGCTTCCTCA GCTTTGCCAA GGGAGACCTC ATCATCCTGG ACCATGACAC GGGCGAGCAG 
GTCATGAACT CGGGCTGGGC CAACGGCATC AATGAGAGGA CCAAGCAGCG TGGGGACTTC 
CCCACCGACA GTGTGTACGT CATGCCCACT GTCACCATGC CACCGCGGGA GATTGTGGCC 
CTGGTCACCA TGACTCCCGA TCAGAGGCAG GACGTTGTCC GGCTCTTGCA GCTGCGAACG 
GCGGAGCCCG AGGTGCGTGC CAAGCCCTAC ACGCTGGAGG AGTTTTCCTA TGACTACTTC 
AGGCCCCCAC CCAAGCACAC GCTGAGCCGT GTCATGGTGT CCAAGGCCCG AGGCAAGGAC 
CGGCTGTGGA GCCACACGCG GGAACCGCTC AAGCAGGCGC TGCTCAAGAA GCTCCTGGGC 
AGTGAGGAGC TCTCGCAGGA GGCCTGCCTG GCCTTCATTG CTGTGCTCAA GTACATGGGC 
GACTACCCGT CCAAGAGGAC ACGCTCCGTC AACGAGCTCA CCGACCAGAT CTTTGAGGGT 
CCCCTGAAAG CCGAGCCCCT GAAGGACGAG GCATATGTGC AGATCCTGAA GCAGCTGACC 
GACAACCACA TCAGGTACAG CGAGGAGCGG GGTTGGGAGC TGCTCTGGCT GTGCACGGGC 
CTTTTCCCAC CCAGCAACAT CCTCCTGCCC CACGTGCAGC GCTTCCTGCA GTCCCGAAAG 
CACTGCCCAC TCGCCATCGA CTGCCTGCAA CGGCTCCAGA AAGCCCTGAG AAACGGGTCC 
CGGAAGTACC CTCCGCACCT GGTGGAGGTG GAGGCCATCC AGCACAAGAC CACCCAGATT 
TTCCACAAAG TCTACTTCCC TGATGACACT GACGAGGCCT TCGAAGTGGA GTCCAGCACC 
AAGGCCAAGG ACTTCTGCCA GAACATCGCC ACCAGGCTGC TCCTCAAGTC CTCAGAGGGA 
TTCAGCCTCT TTGTCAAAAT TGCAGACAAG GTCCTCAGCG TTCCTGAGAA TGACTTCTTC 
TTTGACTTTG TTCGACACTT GACAGACTGG ATAAAGAAAG CTCGGCCCAT CAAGGACGGA 
ATTGTGCCCT CACTCACCTA CCAGGTGTTC TTCATGAAGA AGCTGTGGAC CACCACGGTG 
CCAGGGAAGG ATCCCATGGC CGATTCCATC TTCCACTATT ACCAGGAGTT GCCCAAGTAT 
CTCCGAGGCT ACCACAAGTG CACGCGGGAG GAGGTGCTGC AGCTGGGGGC GCTGATCTAC 
AGGGTCAAGT TCGAGGAGGA CAAGTCCTAC TTCCCCAGCA TCCCCAAGCT GCTGCGGGAG 
CTGGTGCCCC AGGACCTTAT CCGGCAGGTC TCACCTGATG ACTGGAAGCG GTCCATCGTC 
GCCTACTTCA ACAAGCACGC AGGGAAGTCC AAGGAGGAGG CCAAGCTGGC CTTCCTGAAG 
CTCATCTTCA AGTGGCCCAC CTTTGGCTCA GCCTTCTTCG AGGTGAAGGT ACACCATGGG 
CTTCTCAGAG CAGAGGAGGA GGGGAACAGG GCATTGATAA AGCACAGAGA CTCCTCCCTA 
GGACTGTGGG AAAGAGCCAT GGCCTTGGAC ACCTGCCCTG GTGAGGGAAG GAGGGCAGCA 
CCCTCAGCCT TGTCCCAGCT TAGCGTCTTA GCTGGGGTAA GTCCTGGGGA CAGAGGGACC 
AGAAGAAGCT CAGCCTGGCA GTGGAGGCAG ACATGTAAAC AGGCTGGGGT AATGGAGGAT 
GGCCTGTGTT CTGACACTGG GGAGCCCTCG CCCAGGTGGC AGAGCTCGGG AGGCTCTGCC 
GGGTGTGAGA AGGCCTCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873270.1
CDS	16..6696
Translation	MEDPADPADPARIPQPGKWALVRSLNQAFKTYAVLPGDHVWMDLRLGQEFDVPIGAVVKLCDSGQVQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNSRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQALDERNYHVFYCMLEGMSEDQKKKLGLGQASDYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHLGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVDKINAAIYKPPSQDVKNSRRSIGLLDIFGFENFAVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQDALDMIANKPMNIISLIDEESKFPKGTDTTMLHKLNSQHKLNANYIPPKNNHETQFGINHFAGIVYYETQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRRAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLKLKNAATLIQRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLHQQYRLARQRIIQFQARCRAYLVRKAFRHRLWAVLTVQAYARGMIARRLHQRLRAEYLWRLEAEKMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEAARRKKELLEQMERARHEPVNHSDMVDKMFGFLGTSGGLPGQEGQAPSGFEDLERGRREMVEEDLDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGEVTKRLHDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTAKELCNALADKISLKDRFGFSLYIALFDKQRHAQQRGPRPQAQGRYRVAGHSLPGFFSPMLLCFQVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHSPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLNLVPTYIPDREITPLKTLEKWAQLAIAAHKKGIYAQRRTDAQKVKEDVVSYARFKWPLLFSRFYEAYKFSGPSLPKNDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRGAKTTAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDSVYVMPTVTMPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKLLGSEELSQEACLAFIAVLKYMGDYPSKRTRSVNELTDQIFEGPLKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIATRLLLKSSEGFSLFVKIADKVLSVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKVHHGLLRAEEEGNRALIKHRDSSLGLWERAMALDTCPGEGRRAAPSALSQLSVLAGVSPGDRGTRRSSAWQWRQTCKQAGVMEDGLCSDTGEPSPRWQSSGGSAGCEKAS

Target ORF information:

RefSeq Version	XM_017017781.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VIIA (MYO7A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017781.1

ORF Insert Sequence:

ATGGAGGACC CCGCCGACCC TGCCGACCCC GCGCGCATCC CGCAGCCCGG CAAGTGGGCG 
CTGGTGCGCA GCCTGAACCA GGCGTTCAAG ACCTACGCCG TCCTGCCGGG GGACCATGTG 
TGGATGGACC TGAGATTGGG GCAGGAGTTC GACGTGCCCA TCGGGGCGGT GGTGAAGCTC 
TGCGACTCTG GGCAGGTCCA GGTGGTGGAT GATGAAGACA ATGAACACTG GATCTCTCCG 
CAGAACGCAA CGCACATCAA GCCTATGCAC CCCACGTCGG TCCACGGCGT GGAGGACATG 
ATCCGCCTGG GGGACCTCAA CGAGGCGGGC ATCTTGCGCA ACCTGCTTAT CCGCTACCGG 
GACCACCTCA TCTACACGTA TACGGGCTCC ATCCTGGTGG CTGTGAACCC CTACCAGCTG 
CTCTCCATCT ACTCGCCAGA GCACATCCGC CAGTATACCA ACAAGAAGAT TGGGGAGATG 
CCCCCCCACA TCTTTGCCAT TGCTGACAAC TGCTACTTCA ACATGAAACG CAACAGCCGA 
GACCAGTGCT GCATCATCAG TGGGGAATCT GGGGCCGGGA AGACGGAGAG CACAAAGCTG 
ATCCTGCAGT TCCTGGCAGC CATCAGTGGG CAGCACTCGT GGATTGAGCA GCAGGTCTTG 
GAGGCCACCC CCATTCTGGA AGCATTTGGG AATGCCAAGA CCATCCGCAA TGACAACTCA 
AGCCGTTTCG GAAAGTACAT CGACATCCAC TTCAACAAGC GGGGCGCCAT CGAGGGCGCG 
AAGATTGAGC AGTACCTGCT GGAAAAGTCA CGTGTCTGTC GCCAGGCCCT GGATGAAAGG 
AACTACCACG TGTTCTACTG CATGCTGGAG GGTATGAGTG AGGATCAGAA GAAGAAGCTG 
GGCTTGGGCC AGGCCTCTGA CTACAACTAC TTGGCCATGG GTAACTGCAT AACCTGTGAG 
GGCCGGGTGG ACAGCCAGGA GTACGCCAAC ATCCGCTCCG CCATGAAGGT GCTCATGTTC 
ACTGACACCG AGAACTGGGA GATCTCGAAG CTCCTGGCTG CCATCCTGCA CCTGGGCAAC 
CTGCAGTATG AGGCACGCAC ATTTGAAAAC CTGGATGCCT GTGAGGTTCT CTTCTCCCCA 
TCGCTGGCCA CAGCTGCATC CCTGCTTGAG GTGAACCCCC CAGACCTGAT GAGCTGCCTG 
ACTAGCCGCA CCCTCATCAC CCGCGGGGAG ACGGTGTCCA CCCCACTGAG CAGGGAACAG 
GCACTGGACG TGCGCGACGC CTTCGTAAAG GGGATCTACG GGCGGCTGTT CGTGTGGATT 
GTGGACAAGA TCAACGCAGC AATTTACAAG CCTCCCTCCC AGGATGTGAA GAACTCTCGC 
AGGTCCATCG GCCTCCTGGA CATCTTTGGG TTTGAGAACT TTGCTGTGAA CAGCTTTGAG 
CAGCTCTGCA TCAACTTCGC CAATGAGCAC CTGCAGCAGT TCTTTGTGCG GCACGTGTTC 
AAGCTGGAGC AGGAGGAATA TGACCTGGAG AGCATTGACT GGCTGCACAT CGAGTTCACT 
GACAACCAGG ATGCCCTGGA CATGATTGCC AACAAGCCCA TGAACATCAT CTCCCTCATC 
GATGAGGAGA GCAAGTTCCC CAAGGGCACA GACACCACCA TGTTACACAA GCTGAACTCC 
CAGCACAAGC TCAACGCCAA CTACATCCCC CCCAAGAACA ACCATGAGAC CCAGTTTGGC 
ATCAACCATT TTGCAGGCAT CGTCTACTAT GAGACCCAAG GCTTCCTGGA GAAGAACCGA 
GACACCCTGC ATGGGGACAT TATCCAGCTG GTCCACTCCT CCAGGAACAA GTTCATCAAG 
CAGATCTTCC AGGCCGATGT CGCCATGGGC GCCGAGACCA GGAAGCGCTC GCCCACACTT 
AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG CTGATGCGCA CGCTGGGTGC CTGCCAGCCC 
TTCTTTGTGC GATGCATCAA GCCCAATGAG TTCAAGAAGC CCATGCTGTT CGACCGGCAC 
CTGTGCGTGC GCCAGCTGCG GTACTCAGGA ATGATGGAGA CCATCCGAAT CCGCCGAGCT 
GGCTACCCCA TCCGCTACAG CTTCGTAGAG TTTGTGGAGC GGTACCGTGT GCTGCTGCCA 
GGTGTGAAGC CGGCCTACAA GCAGGGCGAC CTCCGCGGGA CTTGCCAGCG CATGGCTGAG 
GCTGTGCTGG GCACCCACGA TGACTGGCAG ATAGGCAAAA CCAAGATCTT TCTGAAGGAC 
CACCATGACA TGCTGCTGGA AGTGGAGCGG GACAAAGCCA TCACCGACAG AGTCATCCTC 
CTTCAGAAAG TCATCCGGGG ATTCAAAGAC AGGTCTAACT TTCTGAAGCT GAAGAACGCT 
GCCACACTGA TCCAGAGGCA CTGGCGGGGT CACAACTGTA GGAAGAACTA CGGGCTGATG 
CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG CACCGCTCCC GGAAGCTGCA CCAGCAGTAC 
CGCCTGGCCC GCCAGCGCAT CATCCAGTTC CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC 
AAGGCCTTCC GCCACCGCCT CTGGGCTGTG CTCACCGTGC AGGCCTATGC CCGGGGCATG 
ATCGCCCGCA GGCTGCACCA ACGCCTCAGG GCTGAGTATC TGTGGCGCCT CGAGGCTGAG 
AAAATGCGGC TGGCGGAGGA AGAGAAGCTT CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG 
GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC CTGGCCCAGC TGGCTCGTGA GGACGCTGAG 
CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG CGGAAGAAGG AGCTCCTGGA GCAGATGGAA 
AGGGCCCGCC ATGAGCCTGT CAATCACTCA GACATGGTGG ACAAGATGTT TGGCTTCCTG 
GGGACTTCAG GTGGCCTGCC AGGCCAGGAG GGCCAGGCAC CTAGTGGCTT TGAGGACCTG 
GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG GACCTGGATG CAGCCCTGCC CCTGCCTGAC 
GAGGATGAGG AGGACCTCTC TGAGTATAAA TTTGCCAAGT TCGCGGCCAC CTACTTCCAG 
GGGACAACCA CGCACTCCTA CACCCGGCGG CCACTCAAAC AGCCACTGCT CTACCATGAC 
GACGAGGGTG ACCAGCTGGC AGCCCTGGCG GTCTGGATCA CCATCCTCCG CTTCATGGGG 
GACCTCCCTG AGCCCAAGTA CCACACAGCC ATGAGTGATG GCAGTGAGAA GATCCCTGTG 
ATGACCAAGA TTTATGAGAC CCTGGGCAAG AAGACGTACA AGAGGGAGCT GCAGGCCCTG 
CAGGGCGAGG GCGAGGTGAC CAAGAGGCTG CATGACGGGG AGTCCACAGT GCAGGGCAAC 
AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATCGGCAAT 
GGCATCCTGC GGCCAGCACT CCGGGACGAG ATCTACTGCC AGATCAGCAA GCAGCTGACC 
CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATTC TCGTGTCTCT CTGCGTGGGC 
TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG TACCTGCGGA ACTTCATCCA CGGGGGCCCG 
CCCGGCTACG CCCCGTACTG TGAGGAGCGC CTGAGAAGGA CCTTTGTCAA TGGGACACGG 
ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC AATCATGTTG 
CCCGTGACAT TCATGGATGG GACCACCAAG ACCCTGCTGA CGGACTCGGC AACCACGGCC 
AAGGAGCTCT GCAACGCGCT GGCCGACAAG ATCTCTCTCA AGGACCGGTT CGGGTTCTCC 
CTCTACATTG CCCTGTTTGA CAAGCAACGT CATGCTCAGC AGAGGGGCCC CCGCCCACAA 
GCCCAGGGGA GGTACCGTGT TGCTGGACAC AGTTTGCCTG GATTTTTCTC ACCGATGCTC 
TTATGTTTCC AGGTGTCCTC CCTGGGCAGC GGCAGTGACC ACGTCATGGA CGCCATCTCC 
CAGTGCGAGC AGTACGCCAA GGAGCAGGGC GCCCAGGAGC GCAACGCCCC CTGGAGGCTC 
TTCTTCCGCA AAGAGGTCTT CACGCCCTGG CACAGCCCCT CCGAGGACAA CGTGGCCACC 
AACCTCATCT ACCAGCAGGT GGTGCGAGGA GTCAAGTTTG GGGAGTACAG GTGTGAGAAG 
GAGGACGACC TGGCTGAGCT GGCCTCCCAG CAGTACTTTG TAGACTATGG CTCTGAGATG 
ATCCTGGAGC GCCTCCTGAA CCTCGTGCCC ACCTACATCC CCGACCGCGA GATCACGCCC 
CTGAAGACGC TGGAGAAGTG GGCCCAGCTG GCCATCGCCG CCCACAAGAA GGGGATTTAT 
GCCCAGAGGA GAACTGATGC CCAGAAGGTC AAAGAGGATG TGGTCAGTTA TGCCCGCTTC 
AAGTGGCCCT TGCTCTTCTC CAGGTTTTAT GAAGCCTACA AATTCTCAGG CCCCAGTCTC 
CCCAAGAACG ACGTCATCGT GGCCGTCAAC TGGACGGGTG TGTACTTTGT GGATGAGCAG 
GAGCAGGTAC TTCTGGAGCT GTCCTTCCCA GAGATCATGG CCGTGTCCAG CAGCAGGGGA 
GCGAAAACGA CGGCCCCCAG CTTCACGCTG GCCACCATCA AGGGGGACGA ATACACCTTC 
ACCTCCAGCA ATGCTGAGGA CATTCGTGAC CTGGTGGTCA CCTTCCTAGA GGGGCTCCGG 
AAGAGATCTA AGTATGTTGT GGCCCTGCAG GATAACCCCA ACCCCGCAGG CGAGGAGTCA 
GGCTTCCTCA GCTTTGCCAA GGGAGACCTC ATCATCCTGG ACCATGACAC GGGCGAGCAG 
GTCATGAACT CGGGCTGGGC CAACGGCATC AATGAGAGGA CCAAGCAGCG TGGGGACTTC 
CCCACCGACA GTGTGTACGT CATGCCCACT GTCACCATGC CACCGCGGGA GATTGTGGCC 
CTGGTCACCA TGACTCCCGA TCAGAGGCAG GACGTTGTCC GGCTCTTGCA GCTGCGAACG 
GCGGAGCCCG AGGTGCGTGC CAAGCCCTAC ACGCTGGAGG AGTTTTCCTA TGACTACTTC 
AGGCCCCCAC CCAAGCACAC GCTGAGCCGT GTCATGGTGT CCAAGGCCCG AGGCAAGGAC 
CGGCTGTGGA GCCACACGCG GGAACCGCTC AAGCAGGCGC TGCTCAAGAA GCTCCTGGGC 
AGTGAGGAGC TCTCGCAGGA GGCCTGCCTG GCCTTCATTG CTGTGCTCAA GTACATGGGC 
GACTACCCGT CCAAGAGGAC ACGCTCCGTC AACGAGCTCA CCGACCAGAT CTTTGAGGGT 
CCCCTGAAAG CCGAGCCCCT GAAGGACGAG GCATATGTGC AGATCCTGAA GCAGCTGACC 
GACAACCACA TCAGGTACAG CGAGGAGCGG GGTTGGGAGC TGCTCTGGCT GTGCACGGGC 
CTTTTCCCAC CCAGCAACAT CCTCCTGCCC CACGTGCAGC GCTTCCTGCA GTCCCGAAAG 
CACTGCCCAC TCGCCATCGA CTGCCTGCAA CGGCTCCAGA AAGCCCTGAG AAACGGGTCC 
CGGAAGTACC CTCCGCACCT GGTGGAGGTG GAGGCCATCC AGCACAAGAC CACCCAGATT 
TTCCACAAAG TCTACTTCCC TGATGACACT GACGAGGCCT TCGAAGTGGA GTCCAGCACC 
AAGGCCAAGG ACTTCTGCCA GAACATCGCC ACCAGGCTGC TCCTCAAGTC CTCAGAGGGA 
TTCAGCCTCT TTGTCAAAAT TGCAGACAAG GTCCTCAGCG TTCCTGAGAA TGACTTCTTC 
TTTGACTTTG TTCGACACTT GACAGACTGG ATAAAGAAAG CTCGGCCCAT CAAGGACGGA 
ATTGTGCCCT CACTCACCTA CCAGGTGTTC TTCATGAAGA AGCTGTGGAC CACCACGGTG 
CCAGGGAAGG ATCCCATGGC CGATTCCATC TTCCACTATT ACCAGGAGTT GCCCAAGTAT 
CTCCGAGGCT ACCACAAGTG CACGCGGGAG GAGGTGCTGC AGCTGGGGGC GCTGATCTAC 
AGGGTCAAGT TCGAGGAGGA CAAGTCCTAC TTCCCCAGCA TCCCCAAGCT GCTGCGGGAG 
CTGGTGCCCC AGGACCTTAT CCGGCAGGTC TCACCTGATG ACTGGAAGCG GTCCATCGTC 
GCCTACTTCA ACAAGCACGC AGGGAAGTCC AAGGAGGAGG CCAAGCTGGC CTTCCTGAAG 
CTCATCTTCA AGTGGCCCAC CTTTGGCTCA GCCTTCTTCG AGGTGAAGGT ACACCATGGG 
CTTCTCAGAG CAGAGGAGGA GGGGAACAGG GCATTGATAA AGCACAGAGA CTCCTCCCTA 
GGACTGTGGG AAAGAGCCAT GGCCTTGGAC ACCTGCCCTG GTGAGGGAAG GAGGGCAGCA 
CCCTCAGCCT TGTCCCAGCT TAGCGTCTTA GCTGGGGTAA GTCCTGGGGA CAGAGGGACC 
AGAAGAAGCT CAGCCTGGCA GTGGAGGCAG ACATGTAAAC AGGCTGGGGT AATGGAGGAT 
GGCCTGTGTT CTGACACTGG GGAGCCCTCG CCCAGGTGGC AGAGCTCGGG AGGCTCTGCC 
GGGTGTGAGA AGGCCTCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO7A ( XM_017017782.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873271.1 Homo sapiens myosin VIIA (MYO7A), transcript variant X7, mRNA.

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CloneID	OHu92259
Clone ID Related Accession (Same CDS sequence)	XM_017017782.1
Accession Version	XM_017017782.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	unconventional myosin-VIIa isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACC CCGCCGACCC TGCCGACCCC GCGCGCATCC CGCAGCCCGG CAAGTGGGCG 
CTGGTGCGCA GCCTGAACCA GGCGTTCAAG ACCTACGCCG TCCTGCCGGG GGACCATGTG 
TGGATGGACC TGAGATTGGG GCAGGAGTTC GACGTGCCCA TCGGGGCGGT GGTGAAGCTC 
TGCGACTCTG GGCAGGTCCA GGTGGTGGAT GATGAAGACA ATGAACACTG GATCTCTCCG 
CAGAACGCAA CGCACATCAA GCCTATGCAC CCCACGTCGG TCCACGGCGT GGAGGACATG 
ATCCGCCTGG GGGACCTCAA CGAGGCGGGC ATCTTGCGCA ACCTGCTTAT CCGCTACCGG 
GACCACCTCA TCTACACGTA TACGGGCTCC ATCCTGGTGG CTGTGAACCC CTACCAGCTG 
CTCTCCATCT ACTCGCCAGA GCACATCCGC CAGTATACCA ACAAGAAGAT TGGGGAGATG 
CCCCCCCACA TCTTTGCCAT TGCTGACAAC TGCTACTTCA ACATGAAACG CAACAGCCGA 
GACCAGTGCT GCATCATCAG TGGGGAATCT GGGGCCGGGA AGACGGAGAG CACAAAGCTG 
ATCCTGCAGT TCCTGGCAGC CATCAGTGGG CAGCACTCGT GGATTGAGCA GCAGGTCTTG 
GAGGCCACCC CCATTCTGGA AGCATTTGGG AATGCCAAGA CCATCCGCAA TGACAACTCA 
AGCCGTTTCG GAAAGTACAT CGACATCCAC TTCAACAAGC GGGGCGCCAT CGAGGGCGCG 
AAGATTGAGC AGTACCTGCT GGAAAAGTCA CGTGTCTGTC GCCAGGCCCT GGATGAAAGG 
AACTACCACG TGTTCTACTG CATGCTGGAG GGTATGAGTG AGGATCAGAA GAAGAAGCTG 
GGCTTGGGCC AGGCCTCTGA CTACAACTAC TTGGCCATGG GTAACTGCAT AACCTGTGAG 
GGCCGGGTGG ACAGCCAGGA GTACGCCAAC ATCCGCTCCG CCATGAAGGT GCTCATGTTC 
ACTGACACCG AGAACTGGGA GATCTCGAAG CTCCTGGCTG CCATCCTGCA CCTGGGCAAC 
CTGCAGTATG AGGCACGCAC ATTTGAAAAC CTGGATGCCT GTGAGGTTCT CTTCTCCCCA 
TCGCTGGCCA CAGCTGCATC CCTGCTTGAG GTGAACCCCC CAGACCTGAT GAGCTGCCTG 
ACTAGCCGCA CCCTCATCAC CCGCGGGGAG ACGGTGTCCA CCCCACTGAG CAGGGAACAG 
GCACTGGACG TGCGCGACGC CTTCGTAAAG GGGATCTACG GGCGGCTGTT CGTGTGGATT 
GTGGACAAGA TCAACGCAGC AATTTACAAG CCTCCCTCCC AGGATGTGAA GAACTCTCGC 
AGGTCCATCG GCCTCCTGGA CATCTTTGGG TTTGAGAACT TTGCTGTGAA CAGCTTTGAG 
CAGCTCTGCA TCAACTTCGC CAATGAGCAC CTGCAGCAGT TCTTTGTGCG GCACGTGTTC 
AAGCTGGAGC AGGAGGAATA TGACCTGGAG AGCATTGACT GGCTGCACAT CGAGTTCACT 
GACAACCAGG ATGCCCTGGA CATGATTGCC AACAAGCCCA TGAACATCAT CTCCCTCATC 
GATGAGGAGA GCAAGTTCCC CAAGGGCACA GACACCACCA TGTTACACAA GCTGAACTCC 
CAGCACAAGC TCAACGCCAA CTACATCCCC CCCAAGAACA ACCATGAGAC CCAGTTTGGC 
ATCAACCATT TTGCAGGCAT CGTCTACTAT GAGACCCAAG GCTTCCTGGA GAAGAACCGA 
GACACCCTGC ATGGGGACAT TATCCAGCTG GTCCACTCCT CCAGGAACAA GTTCATCAAG 
CAGATCTTCC AGGCCGATGT CGCCATGGGC GCCGAGACCA GGAAGCGCTC GCCCACACTT 
AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG CTGATGCGCA CGCTGGGTGC CTGCCAGCCC 
TTCTTTGTGC GATGCATCAA GCCCAATGAG TTCAAGAAGC CCATGCTGTT CGACCGGCAC 
CTGTGCGTGC GCCAGCTGCG GTACTCAGGA ATGATGGAGA CCATCCGAAT CCGCCGAGCT 
GGCTACCCCA TCCGCTACAG CTTCGTAGAG TTTGTGGAGC GGTACCGTGT GCTGCTGCCA 
GGTGTGAAGC CGGCCTACAA GCAGGGCGAC CTCCGCGGGA CTTGCCAGCG CATGGCTGAG 
GCTGTGCTGG GCACCCACGA TGACTGGCAG ATAGGCAAAA CCAAGATCTT TCTGAAGGAC 
CACCATGACA TGCTGCTGGA AGTGGAGCGG GACAAAGCCA TCACCGACAG AGTCATCCTC 
CTTCAGAAAG TCATCCGGGG ATTCAAAGAC AGGTCTAACT TTCTGAAGCT GAAGAACGCT 
GCCACACTGA TCCAGAGGCA CTGGCGGGGT CACAACTGTA GGAAGAACTA CGGGCTGATG 
CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG CACCGCTCCC GGAAGCTGCA CCAGCAGTAC 
CGCCTGGCCC GCCAGCGCAT CATCCAGTTC CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC 
AAGGCCTTCC GCCACCGCCT CTGGGCTGTG CTCACCGTGC AGGCCTATGC CCGGGGCATG 
ATCGCCCGCA GGCTGCACCA ACGCCTCAGG GCTGAGTATC TGTGGCGCCT CGAGGCTGAG 
AAAATGCGGC TGGCGGAGGA AGAGAAGCTT CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG 
GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC CTGGCCCAGC TGGCTCGTGA GGACGCTGAG 
CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG CGGAAGAAGG AGCTCCTGGA GCAGATGGAA 
AGGGCCCGCC ATGAGCCTGT CAATCACTCA GACATGGTGG ACAAGATGTT TGGCTTCCTG 
GGGACTTCAG GTGGCCTGCC AGGCCAGGAG GGCCAGGCAC CTAGTGGCTT TGAGGACCTG 
GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG GACCTGGATG CAGCCCTGCC CCTGCCTGAC 
GAGGATGAGG AGGACCTCTC TGAGTATAAA TTTGCCAAGT TCGCGGCCAC CTACTTCCAG 
GGGACAACCA CGCACTCCTA CACCCGGCGG CCACTCAAAC AGCCACTGCT CTACCATGAC 
GACGAGGGTG ACCAGCTGGC AGCCCTGGCG GTCTGGATCA CCATCCTCCG CTTCATGGGG 
GACCTCCCTG AGCCCAAGTA CCACACAGCC ATGAGTGATG GCAGTGAGAA GATCCCTGTG 
ATGACCAAGA TTTATGAGAC CCTGGGCAAG AAGACGTACA AGAGGGAGCT GCAGGCCCTG 
CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG GGCCAGAAGA AGAGCAGTGT GAGGCACAAG 
CTGGTGCATT TGACTCTGAA AAAGAAGTCC AAGCTCACAG AGGAGGTGAC CAAGAGGCTG 
CATGACGGGG AGTCCACAGT GCAGGGCAAC AGCATGCTGG AGGACCGGCC CACCTCCAAC 
CTGGAGAAGC TGCACTTCAT CATCGGCAAT GGCATCCTGC GGCCAGCACT CCGGGACGAG 
ATCTACTGCC AGATCAGCAA GCAGCTGACC CACAACCCCT CCAAGAGCAG CTATGCCCGG 
GGCTGGATTC TCGTGTCTCT CTGCGTGGGC TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG 
TACCTGCGGA ACTTCATCCA CGGGGGCCCG CCCGGCTACG CCCCGTACTG TGAGGAGCGC 
CTGAGAAGGA CCTTTGTCAA TGGGACACGG ACACAGCCGC CCAGCTGGCT GGAGCTGCAG 
GCCACCAAGT CCAAGAAGCC AATCATGTTG CCCGTGACAT TCATGGATGG GACCACCAAG 
ACCCTGCTGA CGGACTCGGC AACCACGGCC AAGGAGCTCT GCAACGCGCT GGCCGACAAG 
ATCTCTCTCA AGGACCGGTT CGGGTTCTCC CTCTACATTG CCCTGTTTGA CAAGGTGTCC 
TCCCTGGGCA GCGGCAGTGA CCACGTCATG GACGCCATCT CCCAGTGCGA GCAGTACGCC 
AAGGAGCAGG GCGCCCAGGA GCGCAACGCC CCCTGGAGGC TCTTCTTCCG CAAAGAGGTC 
TTCACGCCCT GGCACAGCCC CTCCGAGGAC AACGTGGCCA CCAACCTCAT CTACCAGCAG 
GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC AGGTGTGAGA AGGAGGACGA CCTGGCTGAG 
CTGGCCTCCC AGCAGTACTT TGTAGACTAT GGCTCTGAGA TGATCCTGGA GCGCCTCCTG 
AACCTCGTGC CCACCTACAT CCCCGACCGC GAGATCACGC CCCTGAAGAC GCTGGAGAAG 
TGGGCCCAGC TGGCCATCGC CGCCCACAAG AAGGGGATTT ATGCCCAGAG GAGAACTGAT 
GCCCAGAAGG TCAAAGAGGA TGTGGTCAGT TATGCCCGCT TCAAGTGGCC CTTGCTCTTC 
TCCAGGTTTT ATGAAGCCTA CAAATTCTCA GGCCCCAGTC TCCCCAAGAA CGACGTCATC 
GTGGCCGTCA ACTGGACGGG TGTGTACTTT GTGGATGAGC AGGAGCAGGT ACTTCTGGAG 
CTGTCCTTCC CAGAGATCAT GGCCGTGTCC AGCAGCAGGG GAGCGAAAAC GACGGCCCCC 
AGCTTCACGC TGGCCACCAT CAAGGGGGAC GAATACACCT TCACCTCCAG CAATGCTGAG 
GACATTCGTG ACCTGGTGGT CACCTTCCTA GAGGGGCTCC GGAAGAGATC TAAGTATGTT 
GTGGCCCTGC AGGATAACCC CAACCCCGCA GGCGAGGAGT CAGGCTTCCT CAGCTTTGCC 
AAGGGAGACC TCATCATCCT GGACCATGAC ACGGGCGAGC AGGTCATGAA CTCGGGCTGG 
GCCAACGGCA TCAATGAGAG GACCAAGCAG CGTGGGGACT TCCCCACCGA CAGTGTGTAC 
GTCATGCCCA CTGTCACCAT GCCACCGCGG GAGATTGTGG CCCTGGTCAC CATGACTCCC 
GATCAGAGGC AGGACGTTGT CCGGCTCTTG CAGCTGCGAA CGGCGGAGCC CGAGGTGCGT 
GCCAAGCCCT ACACGCTGGA GGAGTTTTCC TATGACTACT TCAGGCCCCC ACCCAAGCAC 
ACGCTGAGCC GTGTCATGGT GTCCAAGGCC CGAGGCAAGG ACCGGCTGTG GAGCCACACG 
CGGGAACCGC TCAAGCAGGC GCTGCTCAAG AAGCTCCTGG GCAGTGAGGA GCTCTCGCAG 
GAGGCCTGCC TGGCCTTCAT TGCTGTGCTC AAGTACATGG GCGACTACCC GTCCAAGAGG 
ACACGCTCCG TCAACGAGCT CACCGACCAG ATCTTTGAGG GTCCCCTGAA AGCCGAGCCC 
CTGAAGGACG AGGCATATGT GCAGATCCTG AAGCAGCTGA CCGACAACCA CATCAGGTAC 
AGCGAGGAGC GGGGTTGGGA GCTGCTCTGG CTGTGCACGG GCCTTTTCCC ACCCAGCAAC 
ATCCTCCTGC CCCACGTGCA GCGCTTCCTG CAGTCCCGAA AGCACTGCCC ACTCGCCATC 
GACTGCCTGC AACGGCTCCA GAAAGCCCTG AGAAACGGGT CCCGGAAGTA CCCTCCGCAC 
CTGGTGGAGG TGGAGGCCAT CCAGCACAAG ACCACCCAGA TTTTCCACAA AGTCTACTTC 
CCTGATGACA CTGACGAGGC CTTCGAAGTG GAGTCCAGCA CCAAGGCCAA GGACTTCTGC 
CAGAACATCG CCACCAGGCT GCTCCTCAAG TCCTCAGAGG GATTCAGCCT CTTTGTCAAA 
ATTGCAGACA AGGTCCTCAG CGTTCCTGAG AATGACTTCT TCTTTGACTT TGTTCGACAC 
TTGACAGACT GGATAAAGAA AGCTCGGCCC ATCAAGGACG GAATTGTGCC CTCACTCACC 
TACCAGGTGT TCTTCATGAA GAAGCTGTGG ACCACCACGG TGCCAGGGAA GGATCCCATG 
GCCGATTCCA TCTTCCACTA TTACCAGGAG TTGCCCAAGT ATCTCCGAGG CTACCACAAG 
TGCACGCGGG AGGAGGTGCT GCAGCTGGGG GCGCTGATCT ACAGGGTCAA GTTCGAGGAG 
GACAAGTCCT ACTTCCCCAG CATCCCCAAG CTGCTGCGGG AGCTGGTGCC CCAGGACCTT 
ATCCGGCAGG TCTCACCTGA TGACTGGAAG CGGTCCATCG TCGCCTACTT CAACAAGCAC 
GCAGGGAAGT CCAAGGAGGA GGCCAAGCTG GCCTTCCTGA AGCTCATCTT CAAGTGGCCC 
ACCTTTGGCT CAGCCTTCTT CGAGGTGAAG GTACACCATG GGCTTCTCAG AGCAGAGGAG 
GAGGGGAACA GGGCATTGAT AAAGCACAGA GACTCCTCCC TAGGACTGTG GGAAAGAGCC 
ATGGCCTTGG ACACCTGCCC TGGTGAGGGA AGGAGGGCAG CACCCTCAGC CTTGTCCCAG 
CTTAGCGTCT TAGCTGGGGT AAGTCCTGGG GACAGAGGGA CCAGAAGAAG CTCAGCCTGG 
CAGTGGAGGC AGACATGTAA ACAGGCTGGG GTAATGGAGG ATGGCCTGTG TTCTGACACT 
GGGGAGCCCT CGCCCAGGTG GCAGAGCTCG GGAGGCTCTG CCGGGTGTGA GAAGGCCTCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873271.1
CDS	16..6678
Translation	MEDPADPADPARIPQPGKWALVRSLNQAFKTYAVLPGDHVWMDLRLGQEFDVPIGAVVKLCDSGQVQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNSRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQALDERNYHVFYCMLEGMSEDQKKKLGLGQASDYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHLGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVDKINAAIYKPPSQDVKNSRRSIGLLDIFGFENFAVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQDALDMIANKPMNIISLIDEESKFPKGTDTTMLHKLNSQHKLNANYIPPKNNHETQFGINHFAGIVYYETQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRRAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLKLKNAATLIQRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLHQQYRLARQRIIQFQARCRAYLVRKAFRHRLWAVLTVQAYARGMIARRLHQRLRAEYLWRLEAEKMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEAARRKKELLEQMERARHEPVNHSDMVDKMFGFLGTSGGLPGQEGQAPSGFEDLERGRREMVEEDLDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGEAQLPEGQKKSSVRHKLVHLTLKKKSKLTEEVTKRLHDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTAKELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHSPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLNLVPTYIPDREITPLKTLEKWAQLAIAAHKKGIYAQRRTDAQKVKEDVVSYARFKWPLLFSRFYEAYKFSGPSLPKNDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRGAKTTAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDSVYVMPTVTMPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKLLGSEELSQEACLAFIAVLKYMGDYPSKRTRSVNELTDQIFEGPLKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIATRLLLKSSEGFSLFVKIADKVLSVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKVHHGLLRAEEEGNRALIKHRDSSLGLWERAMALDTCPGEGRRAAPSALSQLSVLAGVSPGDRGTRRSSAWQWRQTCKQAGVMEDGLCSDTGEPSPRWQSSGGSAGCEKAS

Target ORF information:

RefSeq Version	XM_017017782.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VIIA (MYO7A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017782.1

ORF Insert Sequence:

ATGGAGGACC CCGCCGACCC TGCCGACCCC GCGCGCATCC CGCAGCCCGG CAAGTGGGCG 
CTGGTGCGCA GCCTGAACCA GGCGTTCAAG ACCTACGCCG TCCTGCCGGG GGACCATGTG 
TGGATGGACC TGAGATTGGG GCAGGAGTTC GACGTGCCCA TCGGGGCGGT GGTGAAGCTC 
TGCGACTCTG GGCAGGTCCA GGTGGTGGAT GATGAAGACA ATGAACACTG GATCTCTCCG 
CAGAACGCAA CGCACATCAA GCCTATGCAC CCCACGTCGG TCCACGGCGT GGAGGACATG 
ATCCGCCTGG GGGACCTCAA CGAGGCGGGC ATCTTGCGCA ACCTGCTTAT CCGCTACCGG 
GACCACCTCA TCTACACGTA TACGGGCTCC ATCCTGGTGG CTGTGAACCC CTACCAGCTG 
CTCTCCATCT ACTCGCCAGA GCACATCCGC CAGTATACCA ACAAGAAGAT TGGGGAGATG 
CCCCCCCACA TCTTTGCCAT TGCTGACAAC TGCTACTTCA ACATGAAACG CAACAGCCGA 
GACCAGTGCT GCATCATCAG TGGGGAATCT GGGGCCGGGA AGACGGAGAG CACAAAGCTG 
ATCCTGCAGT TCCTGGCAGC CATCAGTGGG CAGCACTCGT GGATTGAGCA GCAGGTCTTG 
GAGGCCACCC CCATTCTGGA AGCATTTGGG AATGCCAAGA CCATCCGCAA TGACAACTCA 
AGCCGTTTCG GAAAGTACAT CGACATCCAC TTCAACAAGC GGGGCGCCAT CGAGGGCGCG 
AAGATTGAGC AGTACCTGCT GGAAAAGTCA CGTGTCTGTC GCCAGGCCCT GGATGAAAGG 
AACTACCACG TGTTCTACTG CATGCTGGAG GGTATGAGTG AGGATCAGAA GAAGAAGCTG 
GGCTTGGGCC AGGCCTCTGA CTACAACTAC TTGGCCATGG GTAACTGCAT AACCTGTGAG 
GGCCGGGTGG ACAGCCAGGA GTACGCCAAC ATCCGCTCCG CCATGAAGGT GCTCATGTTC 
ACTGACACCG AGAACTGGGA GATCTCGAAG CTCCTGGCTG CCATCCTGCA CCTGGGCAAC 
CTGCAGTATG AGGCACGCAC ATTTGAAAAC CTGGATGCCT GTGAGGTTCT CTTCTCCCCA 
TCGCTGGCCA CAGCTGCATC CCTGCTTGAG GTGAACCCCC CAGACCTGAT GAGCTGCCTG 
ACTAGCCGCA CCCTCATCAC CCGCGGGGAG ACGGTGTCCA CCCCACTGAG CAGGGAACAG 
GCACTGGACG TGCGCGACGC CTTCGTAAAG GGGATCTACG GGCGGCTGTT CGTGTGGATT 
GTGGACAAGA TCAACGCAGC AATTTACAAG CCTCCCTCCC AGGATGTGAA GAACTCTCGC 
AGGTCCATCG GCCTCCTGGA CATCTTTGGG TTTGAGAACT TTGCTGTGAA CAGCTTTGAG 
CAGCTCTGCA TCAACTTCGC CAATGAGCAC CTGCAGCAGT TCTTTGTGCG GCACGTGTTC 
AAGCTGGAGC AGGAGGAATA TGACCTGGAG AGCATTGACT GGCTGCACAT CGAGTTCACT 
GACAACCAGG ATGCCCTGGA CATGATTGCC AACAAGCCCA TGAACATCAT CTCCCTCATC 
GATGAGGAGA GCAAGTTCCC CAAGGGCACA GACACCACCA TGTTACACAA GCTGAACTCC 
CAGCACAAGC TCAACGCCAA CTACATCCCC CCCAAGAACA ACCATGAGAC CCAGTTTGGC 
ATCAACCATT TTGCAGGCAT CGTCTACTAT GAGACCCAAG GCTTCCTGGA GAAGAACCGA 
GACACCCTGC ATGGGGACAT TATCCAGCTG GTCCACTCCT CCAGGAACAA GTTCATCAAG 
CAGATCTTCC AGGCCGATGT CGCCATGGGC GCCGAGACCA GGAAGCGCTC GCCCACACTT 
AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG CTGATGCGCA CGCTGGGTGC CTGCCAGCCC 
TTCTTTGTGC GATGCATCAA GCCCAATGAG TTCAAGAAGC CCATGCTGTT CGACCGGCAC 
CTGTGCGTGC GCCAGCTGCG GTACTCAGGA ATGATGGAGA CCATCCGAAT CCGCCGAGCT 
GGCTACCCCA TCCGCTACAG CTTCGTAGAG TTTGTGGAGC GGTACCGTGT GCTGCTGCCA 
GGTGTGAAGC CGGCCTACAA GCAGGGCGAC CTCCGCGGGA CTTGCCAGCG CATGGCTGAG 
GCTGTGCTGG GCACCCACGA TGACTGGCAG ATAGGCAAAA CCAAGATCTT TCTGAAGGAC 
CACCATGACA TGCTGCTGGA AGTGGAGCGG GACAAAGCCA TCACCGACAG AGTCATCCTC 
CTTCAGAAAG TCATCCGGGG ATTCAAAGAC AGGTCTAACT TTCTGAAGCT GAAGAACGCT 
GCCACACTGA TCCAGAGGCA CTGGCGGGGT CACAACTGTA GGAAGAACTA CGGGCTGATG 
CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG CACCGCTCCC GGAAGCTGCA CCAGCAGTAC 
CGCCTGGCCC GCCAGCGCAT CATCCAGTTC CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC 
AAGGCCTTCC GCCACCGCCT CTGGGCTGTG CTCACCGTGC AGGCCTATGC CCGGGGCATG 
ATCGCCCGCA GGCTGCACCA ACGCCTCAGG GCTGAGTATC TGTGGCGCCT CGAGGCTGAG 
AAAATGCGGC TGGCGGAGGA AGAGAAGCTT CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG 
GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC CTGGCCCAGC TGGCTCGTGA GGACGCTGAG 
CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG CGGAAGAAGG AGCTCCTGGA GCAGATGGAA 
AGGGCCCGCC ATGAGCCTGT CAATCACTCA GACATGGTGG ACAAGATGTT TGGCTTCCTG 
GGGACTTCAG GTGGCCTGCC AGGCCAGGAG GGCCAGGCAC CTAGTGGCTT TGAGGACCTG 
GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG GACCTGGATG CAGCCCTGCC CCTGCCTGAC 
GAGGATGAGG AGGACCTCTC TGAGTATAAA TTTGCCAAGT TCGCGGCCAC CTACTTCCAG 
GGGACAACCA CGCACTCCTA CACCCGGCGG CCACTCAAAC AGCCACTGCT CTACCATGAC 
GACGAGGGTG ACCAGCTGGC AGCCCTGGCG GTCTGGATCA CCATCCTCCG CTTCATGGGG 
GACCTCCCTG AGCCCAAGTA CCACACAGCC ATGAGTGATG GCAGTGAGAA GATCCCTGTG 
ATGACCAAGA TTTATGAGAC CCTGGGCAAG AAGACGTACA AGAGGGAGCT GCAGGCCCTG 
CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG GGCCAGAAGA AGAGCAGTGT GAGGCACAAG 
CTGGTGCATT TGACTCTGAA AAAGAAGTCC AAGCTCACAG AGGAGGTGAC CAAGAGGCTG 
CATGACGGGG AGTCCACAGT GCAGGGCAAC AGCATGCTGG AGGACCGGCC CACCTCCAAC 
CTGGAGAAGC TGCACTTCAT CATCGGCAAT GGCATCCTGC GGCCAGCACT CCGGGACGAG 
ATCTACTGCC AGATCAGCAA GCAGCTGACC CACAACCCCT CCAAGAGCAG CTATGCCCGG 
GGCTGGATTC TCGTGTCTCT CTGCGTGGGC TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG 
TACCTGCGGA ACTTCATCCA CGGGGGCCCG CCCGGCTACG CCCCGTACTG TGAGGAGCGC 
CTGAGAAGGA CCTTTGTCAA TGGGACACGG ACACAGCCGC CCAGCTGGCT GGAGCTGCAG 
GCCACCAAGT CCAAGAAGCC AATCATGTTG CCCGTGACAT TCATGGATGG GACCACCAAG 
ACCCTGCTGA CGGACTCGGC AACCACGGCC AAGGAGCTCT GCAACGCGCT GGCCGACAAG 
ATCTCTCTCA AGGACCGGTT CGGGTTCTCC CTCTACATTG CCCTGTTTGA CAAGGTGTCC 
TCCCTGGGCA GCGGCAGTGA CCACGTCATG GACGCCATCT CCCAGTGCGA GCAGTACGCC 
AAGGAGCAGG GCGCCCAGGA GCGCAACGCC CCCTGGAGGC TCTTCTTCCG CAAAGAGGTC 
TTCACGCCCT GGCACAGCCC CTCCGAGGAC AACGTGGCCA CCAACCTCAT CTACCAGCAG 
GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC AGGTGTGAGA AGGAGGACGA CCTGGCTGAG 
CTGGCCTCCC AGCAGTACTT TGTAGACTAT GGCTCTGAGA TGATCCTGGA GCGCCTCCTG 
AACCTCGTGC CCACCTACAT CCCCGACCGC GAGATCACGC CCCTGAAGAC GCTGGAGAAG 
TGGGCCCAGC TGGCCATCGC CGCCCACAAG AAGGGGATTT ATGCCCAGAG GAGAACTGAT 
GCCCAGAAGG TCAAAGAGGA TGTGGTCAGT TATGCCCGCT TCAAGTGGCC CTTGCTCTTC 
TCCAGGTTTT ATGAAGCCTA CAAATTCTCA GGCCCCAGTC TCCCCAAGAA CGACGTCATC 
GTGGCCGTCA ACTGGACGGG TGTGTACTTT GTGGATGAGC AGGAGCAGGT ACTTCTGGAG 
CTGTCCTTCC CAGAGATCAT GGCCGTGTCC AGCAGCAGGG GAGCGAAAAC GACGGCCCCC 
AGCTTCACGC TGGCCACCAT CAAGGGGGAC GAATACACCT TCACCTCCAG CAATGCTGAG 
GACATTCGTG ACCTGGTGGT CACCTTCCTA GAGGGGCTCC GGAAGAGATC TAAGTATGTT 
GTGGCCCTGC AGGATAACCC CAACCCCGCA GGCGAGGAGT CAGGCTTCCT CAGCTTTGCC 
AAGGGAGACC TCATCATCCT GGACCATGAC ACGGGCGAGC AGGTCATGAA CTCGGGCTGG 
GCCAACGGCA TCAATGAGAG GACCAAGCAG CGTGGGGACT TCCCCACCGA CAGTGTGTAC 
GTCATGCCCA CTGTCACCAT GCCACCGCGG GAGATTGTGG CCCTGGTCAC CATGACTCCC 
GATCAGAGGC AGGACGTTGT CCGGCTCTTG CAGCTGCGAA CGGCGGAGCC CGAGGTGCGT 
GCCAAGCCCT ACACGCTGGA GGAGTTTTCC TATGACTACT TCAGGCCCCC ACCCAAGCAC 
ACGCTGAGCC GTGTCATGGT GTCCAAGGCC CGAGGCAAGG ACCGGCTGTG GAGCCACACG 
CGGGAACCGC TCAAGCAGGC GCTGCTCAAG AAGCTCCTGG GCAGTGAGGA GCTCTCGCAG 
GAGGCCTGCC TGGCCTTCAT TGCTGTGCTC AAGTACATGG GCGACTACCC GTCCAAGAGG 
ACACGCTCCG TCAACGAGCT CACCGACCAG ATCTTTGAGG GTCCCCTGAA AGCCGAGCCC 
CTGAAGGACG AGGCATATGT GCAGATCCTG AAGCAGCTGA CCGACAACCA CATCAGGTAC 
AGCGAGGAGC GGGGTTGGGA GCTGCTCTGG CTGTGCACGG GCCTTTTCCC ACCCAGCAAC 
ATCCTCCTGC CCCACGTGCA GCGCTTCCTG CAGTCCCGAA AGCACTGCCC ACTCGCCATC 
GACTGCCTGC AACGGCTCCA GAAAGCCCTG AGAAACGGGT CCCGGAAGTA CCCTCCGCAC 
CTGGTGGAGG TGGAGGCCAT CCAGCACAAG ACCACCCAGA TTTTCCACAA AGTCTACTTC 
CCTGATGACA CTGACGAGGC CTTCGAAGTG GAGTCCAGCA CCAAGGCCAA GGACTTCTGC 
CAGAACATCG CCACCAGGCT GCTCCTCAAG TCCTCAGAGG GATTCAGCCT CTTTGTCAAA 
ATTGCAGACA AGGTCCTCAG CGTTCCTGAG AATGACTTCT TCTTTGACTT TGTTCGACAC 
TTGACAGACT GGATAAAGAA AGCTCGGCCC ATCAAGGACG GAATTGTGCC CTCACTCACC 
TACCAGGTGT TCTTCATGAA GAAGCTGTGG ACCACCACGG TGCCAGGGAA GGATCCCATG 
GCCGATTCCA TCTTCCACTA TTACCAGGAG TTGCCCAAGT ATCTCCGAGG CTACCACAAG 
TGCACGCGGG AGGAGGTGCT GCAGCTGGGG GCGCTGATCT ACAGGGTCAA GTTCGAGGAG 
GACAAGTCCT ACTTCCCCAG CATCCCCAAG CTGCTGCGGG AGCTGGTGCC CCAGGACCTT 
ATCCGGCAGG TCTCACCTGA TGACTGGAAG CGGTCCATCG TCGCCTACTT CAACAAGCAC 
GCAGGGAAGT CCAAGGAGGA GGCCAAGCTG GCCTTCCTGA AGCTCATCTT CAAGTGGCCC 
ACCTTTGGCT CAGCCTTCTT CGAGGTGAAG GTACACCATG GGCTTCTCAG AGCAGAGGAG 
GAGGGGAACA GGGCATTGAT AAAGCACAGA GACTCCTCCC TAGGACTGTG GGAAAGAGCC 
ATGGCCTTGG ACACCTGCCC TGGTGAGGGA AGGAGGGCAG CACCCTCAGC CTTGTCCCAG 
CTTAGCGTCT TAGCTGGGGT AAGTCCTGGG GACAGAGGGA CCAGAAGAAG CTCAGCCTGG 
CAGTGGAGGC AGACATGTAA ACAGGCTGGG GTAATGGAGG ATGGCCTGTG TTCTGACACT 
GGGGAGCCCT CGCCCAGGTG GCAGAGCTCG GGAGGCTCTG CCGGGTGTGA GAAGGCCTCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO7A ( XM_017017783.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873272.1 Homo sapiens myosin VIIA (MYO7A), transcript variant X8, mRNA.

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CloneID	OHu92260
Clone ID Related Accession (Same CDS sequence)	XM_017017783.1
Accession Version	XM_017017783.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	unconventional myosin-VIIa isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACC CCGCCGACCC TGCCGACCCC GCGCGCATCC CGCAGCCCGG CAAGTGGGCG 
CTGGTGCGCA GCCTGAACCA GGCGTTCAAG ACCTACGCCG TCCTGCCGGG GGACCATGTG 
TGGATGGACC TGAGATTGGG GCAGGAGTTC GACGTGCCCA TCGGGGCGGT GGTGAAGCTC 
TGCGACTCTG GGCAGGTCCA GGTGGTGGAT GATGAAGACA ATGAACACTG GATCTCTCCG 
CAGAACGCAA CGCACATCAA GCCTATGCAC CCCACGTCGG TCCACGGCGT GGAGGACATG 
ATCCGCCTGG GGGACCTCAA CGAGGCGGGC ATCTTGCGCA ACCTGCTTAT CCGCTACCGG 
GACCACCTCA TCTACACGTA TACGGGCTCC ATCCTGGTGG CTGTGAACCC CTACCAGCTG 
CTCTCCATCT ACTCGCCAGA GCACATCCGC CAGTATACCA ACAAGAAGAT TGGGGAGATG 
CCCCCCCACA TCTTTGCCAT TGCTGACAAC TGCTACTTCA ACATGAAACG CAACAGCCGA 
GACCAGTGCT GCATCATCAG TGGGGAATCT GGGGCCGGGA AGACGGAGAG CACAAAGCTG 
ATCCTGCAGT TCCTGGCAGC CATCAGTGGG CAGCACTCGT GGATTGAGCA GCAGGTCTTG 
GAGGCCACCC CCATTCTGGA AGCATTTGGG AATGCCAAGA CCATCCGCAA TGACAACTCA 
AGCCGTTTCG GAAAGTACAT CGACATCCAC TTCAACAAGC GGGGCGCCAT CGAGGGCGCG 
AAGATTGAGC AGTACCTGCT GGAAAAGTCA CGTGTCTGTC GCCAGGCCCT GGATGAAAGG 
AACTACCACG TGTTCTACTG CATGCTGGAG GGTATGAGTG AGGATCAGAA GAAGAAGCTG 
GGCTTGGGCC AGGCCTCTGA CTACAACTAC TTGGCCATGG GTAACTGCAT AACCTGTGAG 
GGCCGGGTGG ACAGCCAGGA GTACGCCAAC ATCCGCTCCG CCATGAAGGT GCTCATGTTC 
ACTGACACCG AGAACTGGGA GATCTCGAAG CTCCTGGCTG CCATCCTGCA CCTGGGCAAC 
CTGCAGTATG AGGCACGCAC ATTTGAAAAC CTGGATGCCT GTGAGGTTCT CTTCTCCCCA 
TCGCTGGCCA CAGCTGCATC CCTGCTTGAG GTGAACCCCC CAGACCTGAT GAGCTGCCTG 
ACTAGCCGCA CCCTCATCAC CCGCGGGGAG ACGGTGTCCA CCCCACTGAG CAGGGAACAG 
GCACTGGACG TGCGCGACGC CTTCGTAAAG GGGATCTACG GGCGGCTGTT CGTGTGGATT 
GTGGACAAGA TCAACGCAGC AATTTACAAG CCTCCCTCCC AGGATGTGAA GAACTCTCGC 
AGGTCCATCG GCCTCCTGGA CATCTTTGGG TTTGAGAACT TTGCTGTGAA CAGCTTTGAG 
CAGCTCTGCA TCAACTTCGC CAATGAGCAC CTGCAGCAGT TCTTTGTGCG GCACGTGTTC 
AAGCTGGAGC AGGAGGAATA TGACCTGGAG AGCATTGACT GGCTGCACAT CGAGTTCACT 
GACAACCAGG ATGCCCTGGA CATGATTGCC AACAAGCCCA TGAACATCAT CTCCCTCATC 
GATGAGGAGA GCAAGTTCCC CAAGGGCACA GACACCACCA TGTTACACAA GCTGAACTCC 
CAGCACAAGC TCAACGCCAA CTACATCCCC CCCAAGAACA ACCATGAGAC CCAGTTTGGC 
ATCAACCATT TTGCAGGCAT CGTCTACTAT GAGACCCAAG GCTTCCTGGA GAAGAACCGA 
GACACCCTGC ATGGGGACAT TATCCAGCTG GTCCACTCCT CCAGGAACAA GTTCATCAAG 
CAGATCTTCC AGGCCGATGT CGCCATGGGC GCCGAGACCA GGAAGCGCTC GCCCACACTT 
AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG CTGATGCGCA CGCTGGGTGC CTGCCAGCCC 
TTCTTTGTGC GATGCATCAA GCCCAATGAG TTCAAGAAGC CCATGCTGTT CGACCGGCAC 
CTGTGCGTGC GCCAGCTGCG GTACTCAGGA ATGATGGAGA CCATCCGAAT CCGCCGAGCT 
GGCTACCCCA TCCGCTACAG CTTCGTAGAG TTTGTGGAGC GGTACCGTGT GCTGCTGCCA 
GGTGTGAAGC CGGCCTACAA GCAGGGCGAC CTCCGCGGGA CTTGCCAGCG CATGGCTGAG 
GCTGTGCTGG GCACCCACGA TGACTGGCAG ATAGGCAAAA CCAAGATCTT TCTGAAGGAC 
CACCATGACA TGCTGCTGGA AGTGGAGCGG GACAAAGCCA TCACCGACAG AGTCATCCTC 
CTTCAGAAAG TCATCCGGGG ATTCAAAGAC AGGTCTAACT TTCTGAAGCT GAAGAACGCT 
GCCACACTGA TCCAGAGGCA CTGGCGGGGT CACAACTGTA GGAAGAACTA CGGGCTGATG 
CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG CACCGCTCCC GGAAGCTGCA CCAGCAGTAC 
CGCCTGGCCC GCCAGCGCAT CATCCAGTTC CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC 
AAGGCCTTCC GCCACCGCCT CTGGGCTGTG CTCACCGTGC AGGCCTATGC CCGGGGCATG 
ATCGCCCGCA GGCTGCACCA ACGCCTCAGG GCTGAGTATC TGTGGCGCCT CGAGGCTGAG 
AAAATGCGGC TGGCGGAGGA AGAGAAGCTT CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG 
GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC CTGGCCCAGC TGGCTCGTGA GGACGCTGAG 
CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG CGGAAGAAGG AGCTCCTGGA GCAGATGGAA 
AGGGCCCGCC ATGAGCCTGT CAATCACTCA GACATGGTGG ACAAGATGTT TGGCTTCCTG 
GGGACTTCAG GTGGCCTGCC AGGCCAGGAG GGCCAGGCAC CTAGTGGCTT TGAGGACCTG 
GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG GACCTGGATG CAGCCCTGCC CCTGCCTGAC 
GAGGATGAGG AGGACCTCTC TGAGTATAAA TTTGCCAAGT TCGCGGCCAC CTACTTCCAG 
GGGACAACCA CGCACTCCTA CACCCGGCGG CCACTCAAAC AGCCACTGCT CTACCATGAC 
GACGAGGGTG ACCAGCTGGC AGCCCTGGCG GTCTGGATCA CCATCCTCCG CTTCATGGGG 
GACCTCCCTG AGCCCAAGTA CCACACAGCC ATGAGTGATG GCAGTGAGAA GATCCCTGTG 
ATGACCAAGA TTTATGAGAC CCTGGGCAAG AAGACGTACA AGAGGGAGCT GCAGGCCCTG 
CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG GGCCAGAAGA AGAGCAGTGT GAGGCACAAG 
CTGGTGCATT TGACTCTGAA AAAGAAGTCC AAGCTCACAG AGGAGGTGAC CAAGAGGCTG 
CATGACGGGG AGTCCACAGT GCAGGGCAAC AGCATGCTGG AGGACCGGCC CACCTCCAAC 
CTGGAGAAGC TGCACTTCAT CATCGGCAAT GGCATCCTGC GGCCAGCACT CCGGGACGAG 
ATCTACTGCC AGATCAGCAA GCAGCTGACC CACAACCCCT CCAAGAGCAG CTATGCCCGG 
GGCTGGATTC TCGTGTCTCT CTGCGTGGGC TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG 
TACCTGCGGA ACTTCATCCA CGGGGGCCCG CCCGGCTACG CCCCGTACTG TGAGGAGCGC 
CTGAGAAGGA CCTTTGTCAA TGGGACACGG ACACAGCCGC CCAGCTGGCT GGAGCTGCAG 
GCCACCAAGT CCAAGAAGCC AATCATGTTG CCCGTGACAT TCATGGATGG GACCACCAAG 
ACCCTGCTGA CGGACTCGGC AACCACGGCC AAGGAGCTCT GCAACGCGCT GGCCGACAAG 
ATCTCTCTCA AGGACCGGTT CGGGTTCTCC CTCTACATTG CCCTGTTTGA CAAGGTGTCC 
TCCCTGGGCA GCGGCAGTGA CCACGTCATG GACGCCATCT CCCAGTGCGA GCAGTACGCC 
AAGGAGCAGG GCGCCCAGGA GCGCAACGCC CCCTGGAGGC TCTTCTTCCG CAAAGAGGTC 
TTCACGCCCT GGCACAGCCC CTCCGAGGAC AACGTGGCCA CCAACCTCAT CTACCAGCAG 
GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC AGGTGTGAGA AGGAGGACGA CCTGGCTGAG 
CTGGCCTCCC AGCAGTACTT TGTAGACTAT GGCTCTGAGA TGATCCTGGA GCGCCTCCTG 
AACCTCGTGC CCACCTACAT CCCCGACCGC GAGATCACGC CCCTGAAGAC GCTGGAGAAG 
TGGGCCCAGC TGGCCATCGC CGCCCACAAG AAGGGGATTT ATGCCCAGAG GAGAACTGAT 
GCCCAGAAGG TCAAAGAGGA TGTGGTCAGT TATGCCCGCT TCAAGTGGCC CTTGCTCTTC 
TCCAGGTTTT ATGAAGCCTA CAAATTCTCA GGCCCCAGTC TCCCCAAGAA CGACGTCATC 
GTGGCCGTCA ACTGGACGGG TGTGTACTTT GTGGATGAGC AGGAGCAGGT ACTTCTGGAG 
CTGTCCTTCC CAGAGATCAT GGCCGTGTCC AGCAGCAGGG GAGCGAAAAC GACGGCCCCC 
AGCTTCACGC TGGCCACCAT CAAGGGGGAC GAATACACCT TCACCTCCAG CAATGCTGAG 
GACATTCGTG ACCTGGTGGT CACCTTCCTA GAGGGGCTCC GGAAGAGATC TAAGTATGTT 
GTGGCCCTGC AGGATAACCC CAACCCCGGC GAGGAGTCAG GCTTCCTCAG CTTTGCCAAG 
GGAGACCTCA TCATCCTGGA CCATGACACG GGCGAGCAGG TCATGAACTC GGGCTGGGCC 
AACGGCATCA ATGAGAGGAC CAAGCAGCGT GGGGACTTCC CCACCGACAG TGTGTACGTC 
ATGCCCACTG TCACCATGCC ACCGCGGGAG ATTGTGGCCC TGGTCACCAT GACTCCCGAT 
CAGAGGCAGG ACGTTGTCCG GCTCTTGCAG CTGCGAACGG CGGAGCCCGA GGTGCGTGCC 
AAGCCCTACA CGCTGGAGGA GTTTTCCTAT GACTACTTCA GGCCCCCACC CAAGCACACG 
CTGAGCCGTG TCATGGTGTC CAAGGCCCGA GGCAAGGACC GGCTGTGGAG CCACACGCGG 
GAACCGCTCA AGCAGGCGCT GCTCAAGAAG CTCCTGGGCA GTGAGGAGCT CTCGCAGGAG 
GCCTGCCTGG CCTTCATTGC TGTGCTCAAG TACATGGGCG ACTACCCGTC CAAGAGGACA 
CGCTCCGTCA ACGAGCTCAC CGACCAGATC TTTGAGGGTC CCCTGAAAGC CGAGCCCCTG 
AAGGACGAGG CATATGTGCA GATCCTGAAG CAGCTGACCG ACAACCACAT CAGGTACAGC 
GAGGAGCGGG GTTGGGAGCT GCTCTGGCTG TGCACGGGCC TTTTCCCACC CAGCAACATC 
CTCCTGCCCC ACGTGCAGCG CTTCCTGCAG TCCCGAAAGC ACTGCCCACT CGCCATCGAC 
TGCCTGCAAC GGCTCCAGAA AGCCCTGAGA AACGGGTCCC GGAAGTACCC TCCGCACCTG 
GTGGAGGTGG AGGCCATCCA GCACAAGACC ACCCAGATTT TCCACAAAGT CTACTTCCCT 
GATGACACTG ACGAGGCCTT CGAAGTGGAG TCCAGCACCA AGGCCAAGGA CTTCTGCCAG 
AACATCGCCA CCAGGCTGCT CCTCAAGTCC TCAGAGGGAT TCAGCCTCTT TGTCAAAATT 
GCAGACAAGG TCCTCAGCGT TCCTGAGAAT GACTTCTTCT TTGACTTTGT TCGACACTTG 
ACAGACTGGA TAAAGAAAGC TCGGCCCATC AAGGACGGAA TTGTGCCCTC ACTCACCTAC 
CAGGTGTTCT TCATGAAGAA GCTGTGGACC ACCACGGTGC CAGGGAAGGA TCCCATGGCC 
GATTCCATCT TCCACTATTA CCAGGAGTTG CCCAAGTATC TCCGAGGCTA CCACAAGTGC 
ACGCGGGAGG AGGTGCTGCA GCTGGGGGCG CTGATCTACA GGGTCAAGTT CGAGGAGGAC 
AAGTCCTACT TCCCCAGCAT CCCCAAGCTG CTGCGGGAGC TGGTGCCCCA GGACCTTATC 
CGGCAGGTCT CACCTGATGA CTGGAAGCGG TCCATCGTCG CCTACTTCAA CAAGCACGCA 
GGGAAGTCCA AGGAGGAGGC CAAGCTGGCC TTCCTGAAGC TCATCTTCAA GTGGCCCACC 
TTTGGCTCAG CCTTCTTCGA GGTGAAGGTA CACCATGGGC TTCTCAGAGC AGAGGAGGAG 
GGGAACAGGG CATTGATAAA GCACAGAGAC TCCTCCCTAG GACTGTGGGA AAGAGCCATG 
GCCTTGGACA CCTGCCCTGG TGAGGGAAGG AGGGCAGCAC CCTCAGCCTT GTCCCAGCTT 
AGCGTCTTAG CTGGGGTAAG TCCTGGGGAC AGAGGGACCA GAAGAAGCTC AGCCTGGCAG 
TGGAGGCAGA CATGTAAACA GGCTGGGGTA ATGGAGGATG GCCTGTGTTC TGACACTGGG 
GAGCCCTCGC CCAGGTGGCA GAGCTCGGGA GGCTCTGCCG GGTGTGAGAA GGCCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873272.1
CDS	16..6675
Translation	MEDPADPADPARIPQPGKWALVRSLNQAFKTYAVLPGDHVWMDLRLGQEFDVPIGAVVKLCDSGQVQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNSRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQALDERNYHVFYCMLEGMSEDQKKKLGLGQASDYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHLGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVDKINAAIYKPPSQDVKNSRRSIGLLDIFGFENFAVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQDALDMIANKPMNIISLIDEESKFPKGTDTTMLHKLNSQHKLNANYIPPKNNHETQFGINHFAGIVYYETQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRRAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLKLKNAATLIQRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLHQQYRLARQRIIQFQARCRAYLVRKAFRHRLWAVLTVQAYARGMIARRLHQRLRAEYLWRLEAEKMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEAARRKKELLEQMERARHEPVNHSDMVDKMFGFLGTSGGLPGQEGQAPSGFEDLERGRREMVEEDLDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGEAQLPEGQKKSSVRHKLVHLTLKKKSKLTEEVTKRLHDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTAKELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHSPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLNLVPTYIPDREITPLKTLEKWAQLAIAAHKKGIYAQRRTDAQKVKEDVVSYARFKWPLLFSRFYEAYKFSGPSLPKNDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRGAKTTAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDSVYVMPTVTMPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKLLGSEELSQEACLAFIAVLKYMGDYPSKRTRSVNELTDQIFEGPLKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIATRLLLKSSEGFSLFVKIADKVLSVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKVHHGLLRAEEEGNRALIKHRDSSLGLWERAMALDTCPGEGRRAAPSALSQLSVLAGVSPGDRGTRRSSAWQWRQTCKQAGVMEDGLCSDTGEPSPRWQSSGGSAGCEKAS

Target ORF information:

RefSeq Version	XM_017017783.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VIIA (MYO7A), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017783.1

ORF Insert Sequence:

ATGGAGGACC CCGCCGACCC TGCCGACCCC GCGCGCATCC CGCAGCCCGG CAAGTGGGCG 
CTGGTGCGCA GCCTGAACCA GGCGTTCAAG ACCTACGCCG TCCTGCCGGG GGACCATGTG 
TGGATGGACC TGAGATTGGG GCAGGAGTTC GACGTGCCCA TCGGGGCGGT GGTGAAGCTC 
TGCGACTCTG GGCAGGTCCA GGTGGTGGAT GATGAAGACA ATGAACACTG GATCTCTCCG 
CAGAACGCAA CGCACATCAA GCCTATGCAC CCCACGTCGG TCCACGGCGT GGAGGACATG 
ATCCGCCTGG GGGACCTCAA CGAGGCGGGC ATCTTGCGCA ACCTGCTTAT CCGCTACCGG 
GACCACCTCA TCTACACGTA TACGGGCTCC ATCCTGGTGG CTGTGAACCC CTACCAGCTG 
CTCTCCATCT ACTCGCCAGA GCACATCCGC CAGTATACCA ACAAGAAGAT TGGGGAGATG 
CCCCCCCACA TCTTTGCCAT TGCTGACAAC TGCTACTTCA ACATGAAACG CAACAGCCGA 
GACCAGTGCT GCATCATCAG TGGGGAATCT GGGGCCGGGA AGACGGAGAG CACAAAGCTG 
ATCCTGCAGT TCCTGGCAGC CATCAGTGGG CAGCACTCGT GGATTGAGCA GCAGGTCTTG 
GAGGCCACCC CCATTCTGGA AGCATTTGGG AATGCCAAGA CCATCCGCAA TGACAACTCA 
AGCCGTTTCG GAAAGTACAT CGACATCCAC TTCAACAAGC GGGGCGCCAT CGAGGGCGCG 
AAGATTGAGC AGTACCTGCT GGAAAAGTCA CGTGTCTGTC GCCAGGCCCT GGATGAAAGG 
AACTACCACG TGTTCTACTG CATGCTGGAG GGTATGAGTG AGGATCAGAA GAAGAAGCTG 
GGCTTGGGCC AGGCCTCTGA CTACAACTAC TTGGCCATGG GTAACTGCAT AACCTGTGAG 
GGCCGGGTGG ACAGCCAGGA GTACGCCAAC ATCCGCTCCG CCATGAAGGT GCTCATGTTC 
ACTGACACCG AGAACTGGGA GATCTCGAAG CTCCTGGCTG CCATCCTGCA CCTGGGCAAC 
CTGCAGTATG AGGCACGCAC ATTTGAAAAC CTGGATGCCT GTGAGGTTCT CTTCTCCCCA 
TCGCTGGCCA CAGCTGCATC CCTGCTTGAG GTGAACCCCC CAGACCTGAT GAGCTGCCTG 
ACTAGCCGCA CCCTCATCAC CCGCGGGGAG ACGGTGTCCA CCCCACTGAG CAGGGAACAG 
GCACTGGACG TGCGCGACGC CTTCGTAAAG GGGATCTACG GGCGGCTGTT CGTGTGGATT 
GTGGACAAGA TCAACGCAGC AATTTACAAG CCTCCCTCCC AGGATGTGAA GAACTCTCGC 
AGGTCCATCG GCCTCCTGGA CATCTTTGGG TTTGAGAACT TTGCTGTGAA CAGCTTTGAG 
CAGCTCTGCA TCAACTTCGC CAATGAGCAC CTGCAGCAGT TCTTTGTGCG GCACGTGTTC 
AAGCTGGAGC AGGAGGAATA TGACCTGGAG AGCATTGACT GGCTGCACAT CGAGTTCACT 
GACAACCAGG ATGCCCTGGA CATGATTGCC AACAAGCCCA TGAACATCAT CTCCCTCATC 
GATGAGGAGA GCAAGTTCCC CAAGGGCACA GACACCACCA TGTTACACAA GCTGAACTCC 
CAGCACAAGC TCAACGCCAA CTACATCCCC CCCAAGAACA ACCATGAGAC CCAGTTTGGC 
ATCAACCATT TTGCAGGCAT CGTCTACTAT GAGACCCAAG GCTTCCTGGA GAAGAACCGA 
GACACCCTGC ATGGGGACAT TATCCAGCTG GTCCACTCCT CCAGGAACAA GTTCATCAAG 
CAGATCTTCC AGGCCGATGT CGCCATGGGC GCCGAGACCA GGAAGCGCTC GCCCACACTT 
AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG CTGATGCGCA CGCTGGGTGC CTGCCAGCCC 
TTCTTTGTGC GATGCATCAA GCCCAATGAG TTCAAGAAGC CCATGCTGTT CGACCGGCAC 
CTGTGCGTGC GCCAGCTGCG GTACTCAGGA ATGATGGAGA CCATCCGAAT CCGCCGAGCT 
GGCTACCCCA TCCGCTACAG CTTCGTAGAG TTTGTGGAGC GGTACCGTGT GCTGCTGCCA 
GGTGTGAAGC CGGCCTACAA GCAGGGCGAC CTCCGCGGGA CTTGCCAGCG CATGGCTGAG 
GCTGTGCTGG GCACCCACGA TGACTGGCAG ATAGGCAAAA CCAAGATCTT TCTGAAGGAC 
CACCATGACA TGCTGCTGGA AGTGGAGCGG GACAAAGCCA TCACCGACAG AGTCATCCTC 
CTTCAGAAAG TCATCCGGGG ATTCAAAGAC AGGTCTAACT TTCTGAAGCT GAAGAACGCT 
GCCACACTGA TCCAGAGGCA CTGGCGGGGT CACAACTGTA GGAAGAACTA CGGGCTGATG 
CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG CACCGCTCCC GGAAGCTGCA CCAGCAGTAC 
CGCCTGGCCC GCCAGCGCAT CATCCAGTTC CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC 
AAGGCCTTCC GCCACCGCCT CTGGGCTGTG CTCACCGTGC AGGCCTATGC CCGGGGCATG 
ATCGCCCGCA GGCTGCACCA ACGCCTCAGG GCTGAGTATC TGTGGCGCCT CGAGGCTGAG 
AAAATGCGGC TGGCGGAGGA AGAGAAGCTT CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG 
GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC CTGGCCCAGC TGGCTCGTGA GGACGCTGAG 
CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG CGGAAGAAGG AGCTCCTGGA GCAGATGGAA 
AGGGCCCGCC ATGAGCCTGT CAATCACTCA GACATGGTGG ACAAGATGTT TGGCTTCCTG 
GGGACTTCAG GTGGCCTGCC AGGCCAGGAG GGCCAGGCAC CTAGTGGCTT TGAGGACCTG 
GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG GACCTGGATG CAGCCCTGCC CCTGCCTGAC 
GAGGATGAGG AGGACCTCTC TGAGTATAAA TTTGCCAAGT TCGCGGCCAC CTACTTCCAG 
GGGACAACCA CGCACTCCTA CACCCGGCGG CCACTCAAAC AGCCACTGCT CTACCATGAC 
GACGAGGGTG ACCAGCTGGC AGCCCTGGCG GTCTGGATCA CCATCCTCCG CTTCATGGGG 
GACCTCCCTG AGCCCAAGTA CCACACAGCC ATGAGTGATG GCAGTGAGAA GATCCCTGTG 
ATGACCAAGA TTTATGAGAC CCTGGGCAAG AAGACGTACA AGAGGGAGCT GCAGGCCCTG 
CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG GGCCAGAAGA AGAGCAGTGT GAGGCACAAG 
CTGGTGCATT TGACTCTGAA AAAGAAGTCC AAGCTCACAG AGGAGGTGAC CAAGAGGCTG 
CATGACGGGG AGTCCACAGT GCAGGGCAAC AGCATGCTGG AGGACCGGCC CACCTCCAAC 
CTGGAGAAGC TGCACTTCAT CATCGGCAAT GGCATCCTGC GGCCAGCACT CCGGGACGAG 
ATCTACTGCC AGATCAGCAA GCAGCTGACC CACAACCCCT CCAAGAGCAG CTATGCCCGG 
GGCTGGATTC TCGTGTCTCT CTGCGTGGGC TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG 
TACCTGCGGA ACTTCATCCA CGGGGGCCCG CCCGGCTACG CCCCGTACTG TGAGGAGCGC 
CTGAGAAGGA CCTTTGTCAA TGGGACACGG ACACAGCCGC CCAGCTGGCT GGAGCTGCAG 
GCCACCAAGT CCAAGAAGCC AATCATGTTG CCCGTGACAT TCATGGATGG GACCACCAAG 
ACCCTGCTGA CGGACTCGGC AACCACGGCC AAGGAGCTCT GCAACGCGCT GGCCGACAAG 
ATCTCTCTCA AGGACCGGTT CGGGTTCTCC CTCTACATTG CCCTGTTTGA CAAGGTGTCC 
TCCCTGGGCA GCGGCAGTGA CCACGTCATG GACGCCATCT CCCAGTGCGA GCAGTACGCC 
AAGGAGCAGG GCGCCCAGGA GCGCAACGCC CCCTGGAGGC TCTTCTTCCG CAAAGAGGTC 
TTCACGCCCT GGCACAGCCC CTCCGAGGAC AACGTGGCCA CCAACCTCAT CTACCAGCAG 
GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC AGGTGTGAGA AGGAGGACGA CCTGGCTGAG 
CTGGCCTCCC AGCAGTACTT TGTAGACTAT GGCTCTGAGA TGATCCTGGA GCGCCTCCTG 
AACCTCGTGC CCACCTACAT CCCCGACCGC GAGATCACGC CCCTGAAGAC GCTGGAGAAG 
TGGGCCCAGC TGGCCATCGC CGCCCACAAG AAGGGGATTT ATGCCCAGAG GAGAACTGAT 
GCCCAGAAGG TCAAAGAGGA TGTGGTCAGT TATGCCCGCT TCAAGTGGCC CTTGCTCTTC 
TCCAGGTTTT ATGAAGCCTA CAAATTCTCA GGCCCCAGTC TCCCCAAGAA CGACGTCATC 
GTGGCCGTCA ACTGGACGGG TGTGTACTTT GTGGATGAGC AGGAGCAGGT ACTTCTGGAG 
CTGTCCTTCC CAGAGATCAT GGCCGTGTCC AGCAGCAGGG GAGCGAAAAC GACGGCCCCC 
AGCTTCACGC TGGCCACCAT CAAGGGGGAC GAATACACCT TCACCTCCAG CAATGCTGAG 
GACATTCGTG ACCTGGTGGT CACCTTCCTA GAGGGGCTCC GGAAGAGATC TAAGTATGTT 
GTGGCCCTGC AGGATAACCC CAACCCCGGC GAGGAGTCAG GCTTCCTCAG CTTTGCCAAG 
GGAGACCTCA TCATCCTGGA CCATGACACG GGCGAGCAGG TCATGAACTC GGGCTGGGCC 
AACGGCATCA ATGAGAGGAC CAAGCAGCGT GGGGACTTCC CCACCGACAG TGTGTACGTC 
ATGCCCACTG TCACCATGCC ACCGCGGGAG ATTGTGGCCC TGGTCACCAT GACTCCCGAT 
CAGAGGCAGG ACGTTGTCCG GCTCTTGCAG CTGCGAACGG CGGAGCCCGA GGTGCGTGCC 
AAGCCCTACA CGCTGGAGGA GTTTTCCTAT GACTACTTCA GGCCCCCACC CAAGCACACG 
CTGAGCCGTG TCATGGTGTC CAAGGCCCGA GGCAAGGACC GGCTGTGGAG CCACACGCGG 
GAACCGCTCA AGCAGGCGCT GCTCAAGAAG CTCCTGGGCA GTGAGGAGCT CTCGCAGGAG 
GCCTGCCTGG CCTTCATTGC TGTGCTCAAG TACATGGGCG ACTACCCGTC CAAGAGGACA 
CGCTCCGTCA ACGAGCTCAC CGACCAGATC TTTGAGGGTC CCCTGAAAGC CGAGCCCCTG 
AAGGACGAGG CATATGTGCA GATCCTGAAG CAGCTGACCG ACAACCACAT CAGGTACAGC 
GAGGAGCGGG GTTGGGAGCT GCTCTGGCTG TGCACGGGCC TTTTCCCACC CAGCAACATC 
CTCCTGCCCC ACGTGCAGCG CTTCCTGCAG TCCCGAAAGC ACTGCCCACT CGCCATCGAC 
TGCCTGCAAC GGCTCCAGAA AGCCCTGAGA AACGGGTCCC GGAAGTACCC TCCGCACCTG 
GTGGAGGTGG AGGCCATCCA GCACAAGACC ACCCAGATTT TCCACAAAGT CTACTTCCCT 
GATGACACTG ACGAGGCCTT CGAAGTGGAG TCCAGCACCA AGGCCAAGGA CTTCTGCCAG 
AACATCGCCA CCAGGCTGCT CCTCAAGTCC TCAGAGGGAT TCAGCCTCTT TGTCAAAATT 
GCAGACAAGG TCCTCAGCGT TCCTGAGAAT GACTTCTTCT TTGACTTTGT TCGACACTTG 
ACAGACTGGA TAAAGAAAGC TCGGCCCATC AAGGACGGAA TTGTGCCCTC ACTCACCTAC 
CAGGTGTTCT TCATGAAGAA GCTGTGGACC ACCACGGTGC CAGGGAAGGA TCCCATGGCC 
GATTCCATCT TCCACTATTA CCAGGAGTTG CCCAAGTATC TCCGAGGCTA CCACAAGTGC 
ACGCGGGAGG AGGTGCTGCA GCTGGGGGCG CTGATCTACA GGGTCAAGTT CGAGGAGGAC 
AAGTCCTACT TCCCCAGCAT CCCCAAGCTG CTGCGGGAGC TGGTGCCCCA GGACCTTATC 
CGGCAGGTCT CACCTGATGA CTGGAAGCGG TCCATCGTCG CCTACTTCAA CAAGCACGCA 
GGGAAGTCCA AGGAGGAGGC CAAGCTGGCC TTCCTGAAGC TCATCTTCAA GTGGCCCACC 
TTTGGCTCAG CCTTCTTCGA GGTGAAGGTA CACCATGGGC TTCTCAGAGC AGAGGAGGAG 
GGGAACAGGG CATTGATAAA GCACAGAGAC TCCTCCCTAG GACTGTGGGA AAGAGCCATG 
GCCTTGGACA CCTGCCCTGG TGAGGGAAGG AGGGCAGCAC CCTCAGCCTT GTCCCAGCTT 
AGCGTCTTAG CTGGGGTAAG TCCTGGGGAC AGAGGGACCA GAAGAAGCTC AGCCTGGCAG 
TGGAGGCAGA CATGTAAACA GGCTGGGGTA ATGGAGGATG GCCTGTGTTC TGACACTGGG 
GAGCCCTCGC CCAGGTGGCA GAGCTCGGGA GGCTCTGCCG GGTGTGAGAA GGCCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO7A ( XM_017017784.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873273.1 Homo sapiens myosin VIIA (MYO7A), transcript variant X9, mRNA.

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CloneID	OHu92261
Clone ID Related Accession (Same CDS sequence)	XM_017017784.1
Accession Version	XM_017017784.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	unconventional myosin-VIIa isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACC CCGCCGACCC TGCCGACCCC GCGCGCATCC CGCAGCCCGG CAAGTGGGCG 
CTGGTGCGCA GCCTGAACCA GGCGTTCAAG ACCTACGCCG TCCTGCCGGG GGACCATGTG 
TGGATGGACC TGAGATTGGG GCAGGAGTTC GACGTGCCCA TCGGGGCGGT GGTGAAGCTC 
TGCGACTCTG GGCAGGTCCA GGTGGTGGAT GATGAAGACA ATGAACACTG GATCTCTCCG 
CAGAACGCAA CGCACATCAA GCCTATGCAC CCCACGTCGG TCCACGGCGT GGAGGACATG 
ATCCGCCTGG GGGACCTCAA CGAGGCGGGC ATCTTGCGCA ACCTGCTTAT CCGCTACCGG 
GACCACCTCA TCTACACGTA TACGGGCTCC ATCCTGGTGG CTGTGAACCC CTACCAGCTG 
CTCTCCATCT ACTCGCCAGA GCACATCCGC CAGTATACCA ACAAGAAGAT TGGGGAGATG 
CCCCCCCACA TCTTTGCCAT TGCTGACAAC TGCTACTTCA ACATGAAACG CAACAGCCGA 
GACCAGTGCT GCATCATCAG TGGGGAATCT GGGGCCGGGA AGACGGAGAG CACAAAGCTG 
ATCCTGCAGT TCCTGGCAGC CATCAGTGGG CAGCACTCGT GGATTGAGCA GCAGGTCTTG 
GAGGCCACCC CCATTCTGGA AGCATTTGGG AATGCCAAGA CCATCCGCAA TGACAACTCA 
AGCCGTTTCG GAAAGTACAT CGACATCCAC TTCAACAAGC GGGGCGCCAT CGAGGGCGCG 
AAGATTGAGC AGTACCTGCT GGAAAAGTCA CGTGTCTGTC GCCAGGCCCT GGATGAAAGG 
AACTACCACG TGTTCTACTG CATGCTGGAG GGTATGAGTG AGGATCAGAA GAAGAAGCTG 
GGCTTGGGCC AGGCCTCTGA CTACAACTAC TTGGCCATGG GTAACTGCAT AACCTGTGAG 
GGCCGGGTGG ACAGCCAGGA GTACGCCAAC ATCCGCTCCG CCATGAAGGT GCTCATGTTC 
ACTGACACCG AGAACTGGGA GATCTCGAAG CTCCTGGCTG CCATCCTGCA CCTGGGCAAC 
CTGCAGTATG AGGCACGCAC ATTTGAAAAC CTGGATGCCT GTGAGGTTCT CTTCTCCCCA 
TCGCTGGCCA CAGCTGCATC CCTGCTTGAG GTGAACCCCC CAGACCTGAT GAGCTGCCTG 
ACTAGCCGCA CCCTCATCAC CCGCGGGGAG ACGGTGTCCA CCCCACTGAG CAGGGAACAG 
GCACTGGACG TGCGCGACGC CTTCGTAAAG GGGATCTACG GGCGGCTGTT CGTGTGGATT 
GTGGACAAGA TCAACGCAGC AATTTACAAG CCTCCCTCCC AGGATGTGAA GAACTCTCGC 
AGGTCCATCG GCCTCCTGGA CATCTTTGGG TTTGAGAACT TTGCTGTGAA CAGCTTTGAG 
CAGCTCTGCA TCAACTTCGC CAATGAGCAC CTGCAGCAGT TCTTTGTGCG GCACGTGTTC 
AAGCTGGAGC AGGAGGAATA TGACCTGGAG AGCATTGACT GGCTGCACAT CGAGTTCACT 
GACAACCAGG ATGCCCTGGA CATGATTGCC AACAAGCCCA TGAACATCAT CTCCCTCATC 
GATGAGGAGA GCAAGTTCCC CAAGGGCACA GACACCACCA TGTTACACAA GCTGAACTCC 
CAGCACAAGC TCAACGCCAA CTACATCCCC CCCAAGAACA ACCATGAGAC CCAGTTTGGC 
ATCAACCATT TTGCAGGCAT CGTCTACTAT GAGACCCAAG GCTTCCTGGA GAAGAACCGA 
GACACCCTGC ATGGGGACAT TATCCAGCTG GTCCACTCCT CCAGGAACAA GTTCATCAAG 
CAGATCTTCC AGGCCGATGT CGCCATGGGC GCCGAGACCA GGAAGCGCTC GCCCACACTT 
AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG CTGATGCGCA CGCTGGGTGC CTGCCAGCCC 
TTCTTTGTGC GATGCATCAA GCCCAATGAG TTCAAGAAGC CCATGCTGTT CGACCGGCAC 
CTGTGCGTGC GCCAGCTGCG GTACTCAGGA ATGATGGAGA CCATCCGAAT CCGCCGAGCT 
GGCTACCCCA TCCGCTACAG CTTCGTAGAG TTTGTGGAGC GGTACCGTGT GCTGCTGCCA 
GGTGTGAAGC CGGCCTACAA GCAGGGCGAC CTCCGCGGGA CTTGCCAGCG CATGGCTGAG 
GCTGTGCTGG GCACCCACGA TGACTGGCAG ATAGGCAAAA CCAAGATCTT TCTGAAGGAC 
CACCATGACA TGCTGCTGGA AGTGGAGCGG GACAAAGCCA TCACCGACAG AGTCATCCTC 
CTTCAGAAAG TCATCCGGGG ATTCAAAGAC AGGTCTAACT TTCTGAAGCT GAAGAACGCT 
GCCACACTGA TCCAGAGGCA CTGGCGGGGT CACAACTGTA GGAAGAACTA CGGGCTGATG 
CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG CACCGCTCCC GGAAGCTGCA CCAGCAGTAC 
CGCCTGGCCC GCCAGCGCAT CATCCAGTTC CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC 
AAGGCCTTCC GCCACCGCCT CTGGGCTGTG CTCACCGTGC AGGCCTATGC CCGGGGCATG 
ATCGCCCGCA GGCTGCACCA ACGCCTCAGG GCTGAGTATC TGTGGCGCCT CGAGGCTGAG 
AAAATGCGGC TGGCGGAGGA AGAGAAGCTT CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG 
GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC CTGGCCCAGC TGGCTCGTGA GGACGCTGAG 
CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG CGGAAGAAGG AGCTCCTGGA GCAGATGGAA 
AGGGCCCGCC ATGAGCCTGT CAATCACTCA GACATGGTGG ACAAGATGTT TGGCTTCCTG 
GGGACTTCAG GTGGCCTGCC AGGCCAGGAG GGCCAGGCAC CTAGTGGCTT TGAGGACCTG 
GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG GACCTGGATG CAGCCCTGCC CCTGCCTGAC 
GAGGATGAGG AGGACCTCTC TGAGTATAAA TTTGCCAAGT TCGCGGCCAC CTACTTCCAG 
GGGACAACCA CGCACTCCTA CACCCGGCGG CCACTCAAAC AGCCACTGCT CTACCATGAC 
GACGAGGGTG ACCAGCTGGC AGCCCTGGCG GTCTGGATCA CCATCCTCCG CTTCATGGGG 
GACCTCCCTG AGCCCAAGTA CCACACAGCC ATGAGTGATG GCAGTGAGAA GATCCCTGTG 
ATGACCAAGA TTTATGAGAC CCTGGGCAAG AAGACGTACA AGAGGGAGCT GCAGGCCCTG 
CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG GGCCAGAAGA AGAGCAGTGT GAGGCACAAG 
CTGGTGCATT TGACTCTGAA AAAGAAGTCC AAGCTCACAG AGGAGGTGAC CAAGAGGCTG 
CATGACGGGG AGTCCACAGT GCAGGGCAAC AGCATGCTGG AGGACCGGCC CACCTCCAAC 
CTGGAGAAGC TGCACTTCAT CATCGGCAAT GGCATCCTGC GGCCAGCACT CCGGGACGAG 
ATCTACTGCC AGATCAGCAA GCAGCTGACC CACAACCCCT CCAAGAGCAG CTATGCCCGG 
GGCTGGATTC TCGTGTCTCT CTGCGTGGGC TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG 
TACCTGCGGA ACTTCATCCA CGGGGGCCCG CCCGGCTACG CCCCGTACTG TGAGGAGCGC 
CTGAGAAGGA CCTTTGTCAA TGGGACACGG ACACAGCCGC CCAGCTGGCT GGAGCTGCAG 
GCCACCAAGT CCAAGAAGCC AATCATGTTG CCCGTGACAT TCATGGATGG GACCACCAAG 
ACCCTGCTGA CGGACTCGGC AACCACGGCC AAGGAGCTCT GCAACGCGCT GGCCGACAAG 
ATCTCTCTCA AGGACCGGTT CGGGTTCTCC CTCTACATTG CCCTGTTTGA CAAGGTGTCC 
TCCCTGGGCA GCGGCAGTGA CCACGTCATG GACGCCATCT CCCAGTGCGA GCAGTACGCC 
AAGGAGCAGG GCGCCCAGGA GCGCAACGCC CCCTGGAGGC TCTTCTTCCG CAAAGAGGTC 
TTCACGCCCT GGCACAGCCC CTCCGAGGAC AACGTGGCCA CCAACCTCAT CTACCAGCAG 
GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC AGGTGTGAGA AGGAGGACGA CCTGGCTGAG 
CTGGCCTCCC AGCAGTACTT TGTAGACTAT GGCTCTGAGA TGATCCTGGA GCGCCTCCTG 
AACCTCGTGC CCACCTACAT CCCCGACCGC GAGATCACGC CCCTGAAGAC GCTGGAGAAG 
TGGGCCCAGC TGGCCATCGC CGCCCACAAG AAGGGGATTT ATGCCCAGAG GAGAACTGAT 
GCCCAGAAGG TCAAAGAGGA TGTGGTCAGT TATGCCCGCT TCAAGTGGCC CTTGCTCTTC 
TCCAGGTTTT ATGAAGCCTA CAAATTCTCA GGCCCCAGTC TCCCCAAGAA CGACGTCATC 
GTGGCCGTCA ACTGGACGGG TGTGTACTTT GTGGATGAGC AGGAGCAGGT ACTTCTGGAG 
CTGTCCTTCC CAGAGATCAT GGCCGTGTCC AGCAGCAGGG GAGCGAAAAC GACGGCCCCC 
AGCTTCACGC TGGCCACCAT CAAGGGGGAC GAATACACCT TCACCTCCAG CAATGCTGAG 
GACATTCGTG ACCTGGTGGT CACCTTCCTA GAGGGGCTCC GGAAGAGATC TAAGTATGTT 
GTGGCCCTGC AGGATAACCC CAACCCCGGC GAGGAGTCAG GCTTCCTCAG CTTTGCCAAG 
GGAGACCTCA TCATCCTGGA CCATGACACG GGCGAGCAGG TCATGAACTC GGGCTGGGCC 
AACGGCATCA ATGAGAGGAC CAAGCAGCGT GGGGACTTCC CCACCGACAG TGTGTACGTC 
ATGCCCACTG TCACCATGCC ACCGCGGGAG ATTGTGGCCC TGGTCACCAT GACTCCCGAT 
CAGAGGCAGG ACGTTGTCCG GCTCTTGCAG CTGCGAACGG CGGAGCCCGA GGTGCGTGCC 
AAGCCCTACA CGCTGGAGGA GTTTTCCTAT GACTACTTCA GGCCCCCACC CAAGCACACG 
CTGAGCCGTG TCATGGTGTC CAAGGCCCGA GGCAAGGACC GGCTGTGGAG CCACACGCGG 
GAACCGCTCA AGCAGGCGCT GCTCAAGAAG CTCCTGGGCA GTGAGGAGCT CTCGCAGGAG 
GCCTGCCTGG CCTTCATTGC TGTGCTCAAG TACATGGGCG ACTACCCGTC CAAGAGGACA 
CGCTCCGTCA ACGAGCTCAC CGACCAGATC TTTGAGGGTC CCCTGAAAGC CGAGCCCCTG 
AAGGACGAGG CATATGTGCA GATCCTGAAG CAGCTGACCG ACAACCACAT CAGGTACAGC 
GAGGAGCGGG GTTGGGAGCT GCTCTGGCTG TGCACGGGCC TTTTCCCACC CAGCAACATC 
CTCCTGCCCC ACGTGCAGCG CTTCCTGCAG TCCCGAAAGC ACTGCCCACT CGCCATCGAC 
TGCCTGCAAC GGCTCCAGAA AGCCCTGAGA AACGGGTCCC GGAAGTACCC TCCGCACCTG 
GTGGAGGTGG AGGCCATCCA GCACAAGACC ACCCAGATTT TCCACAAAGT CTACTTCCCT 
GATGACACTG ACGAGGCCTT CGAAGTGGAG TCCAGCACCA AGGCCAAGGA CTTCTGCCAG 
AACATCGCCA CCAGGCTGCT CCTCAAGTCC TCAGAGGGAT TCAGCCTCTT TGTCAAAATT 
GCAGACAAGG TCCTCAGCGT TCCTGAGAAT GACTTCTTCT TTGACTTTGT TCGACACTTG 
ACAGACTGGA TAAAGAAAGC TCGGCCCATC AAGGACGGAA TTGTGCCCTC ACTCACCTAC 
CAGGTGTTCT TCATGAAGAA GCTGTGGACC ACCACGGTGC CAGGGAAGGA TCCCATGGCC 
GATTCCATCT TCCACTATTA CCAGGAGTTG CCCAAGTATC TCCGAGGCTA CCACAAGTGC 
ACGCGGGAGG AGGTGCTGCA GCTGGGGGCG CTGATCTACA GGGTCAAGTT CGAGGAGGAC 
AAGTCCTACT TCCCCAGCAT CCCCAAGCTG CTGCGGGAGC TGGTGCCCCA GGACCTTATC 
CGGCAGGTCT CACCTGATGA CTGGAAGCGG TCCATCGTCG CCTACTTCAA CAAGCACGCA 
GGGAAGTCCA AGGAGGAGGC CAAGCTGGCC TTCCTGAAGC TCATCTTCAA GTGGCCCACC 
TTTGGCTCAG CCTTCTTCGA GGTGAAGCAA ACTACGGAGC CAAACTTCCC TGAGATCCTC 
CTAATTGCCA TCAACAAGTA TGGGGTCAGC CTCATCGATC CCAAAACGAA GGATATCCTC 
ACCACTCATC CCTTCACCAA GATCTCCAAC TGGAGCAGCG GCAACACCTA CTTCCACATC 
ACCATTGGGA ACTTGGTGCG CGGGAGCAAA CTGCTCTGCG AGACGTCACT GGGCTACAAG 
ATGGATGACC TCCTGACTTC CTACATTAGC CAGATGCTCA CAGCCATGAG CAAACAGCGG 
GGCTCCAGGA GCGGCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873273.1
CDS	16..6636
Translation	MEDPADPADPARIPQPGKWALVRSLNQAFKTYAVLPGDHVWMDLRLGQEFDVPIGAVVKLCDSGQVQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNSRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQALDERNYHVFYCMLEGMSEDQKKKLGLGQASDYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHLGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVDKINAAIYKPPSQDVKNSRRSIGLLDIFGFENFAVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQDALDMIANKPMNIISLIDEESKFPKGTDTTMLHKLNSQHKLNANYIPPKNNHETQFGINHFAGIVYYETQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRRAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLKLKNAATLIQRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLHQQYRLARQRIIQFQARCRAYLVRKAFRHRLWAVLTVQAYARGMIARRLHQRLRAEYLWRLEAEKMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEAARRKKELLEQMERARHEPVNHSDMVDKMFGFLGTSGGLPGQEGQAPSGFEDLERGRREMVEEDLDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGEAQLPEGQKKSSVRHKLVHLTLKKKSKLTEEVTKRLHDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTAKELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHSPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLNLVPTYIPDREITPLKTLEKWAQLAIAAHKKGIYAQRRTDAQKVKEDVVSYARFKWPLLFSRFYEAYKFSGPSLPKNDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRGAKTTAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDSVYVMPTVTMPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKLLGSEELSQEACLAFIAVLKYMGDYPSKRTRSVNELTDQIFEGPLKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIATRLLLKSSEGFSLFVKIADKVLSVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKQTTEPNFPEILLIAINKYGVSLIDPKTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRGSRSGK

Target ORF information:

RefSeq Version	XM_017017784.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VIIA (MYO7A), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017784.1

ORF Insert Sequence:

ATGGAGGACC CCGCCGACCC TGCCGACCCC GCGCGCATCC CGCAGCCCGG CAAGTGGGCG 
CTGGTGCGCA GCCTGAACCA GGCGTTCAAG ACCTACGCCG TCCTGCCGGG GGACCATGTG 
TGGATGGACC TGAGATTGGG GCAGGAGTTC GACGTGCCCA TCGGGGCGGT GGTGAAGCTC 
TGCGACTCTG GGCAGGTCCA GGTGGTGGAT GATGAAGACA ATGAACACTG GATCTCTCCG 
CAGAACGCAA CGCACATCAA GCCTATGCAC CCCACGTCGG TCCACGGCGT GGAGGACATG 
ATCCGCCTGG GGGACCTCAA CGAGGCGGGC ATCTTGCGCA ACCTGCTTAT CCGCTACCGG 
GACCACCTCA TCTACACGTA TACGGGCTCC ATCCTGGTGG CTGTGAACCC CTACCAGCTG 
CTCTCCATCT ACTCGCCAGA GCACATCCGC CAGTATACCA ACAAGAAGAT TGGGGAGATG 
CCCCCCCACA TCTTTGCCAT TGCTGACAAC TGCTACTTCA ACATGAAACG CAACAGCCGA 
GACCAGTGCT GCATCATCAG TGGGGAATCT GGGGCCGGGA AGACGGAGAG CACAAAGCTG 
ATCCTGCAGT TCCTGGCAGC CATCAGTGGG CAGCACTCGT GGATTGAGCA GCAGGTCTTG 
GAGGCCACCC CCATTCTGGA AGCATTTGGG AATGCCAAGA CCATCCGCAA TGACAACTCA 
AGCCGTTTCG GAAAGTACAT CGACATCCAC TTCAACAAGC GGGGCGCCAT CGAGGGCGCG 
AAGATTGAGC AGTACCTGCT GGAAAAGTCA CGTGTCTGTC GCCAGGCCCT GGATGAAAGG 
AACTACCACG TGTTCTACTG CATGCTGGAG GGTATGAGTG AGGATCAGAA GAAGAAGCTG 
GGCTTGGGCC AGGCCTCTGA CTACAACTAC TTGGCCATGG GTAACTGCAT AACCTGTGAG 
GGCCGGGTGG ACAGCCAGGA GTACGCCAAC ATCCGCTCCG CCATGAAGGT GCTCATGTTC 
ACTGACACCG AGAACTGGGA GATCTCGAAG CTCCTGGCTG CCATCCTGCA CCTGGGCAAC 
CTGCAGTATG AGGCACGCAC ATTTGAAAAC CTGGATGCCT GTGAGGTTCT CTTCTCCCCA 
TCGCTGGCCA CAGCTGCATC CCTGCTTGAG GTGAACCCCC CAGACCTGAT GAGCTGCCTG 
ACTAGCCGCA CCCTCATCAC CCGCGGGGAG ACGGTGTCCA CCCCACTGAG CAGGGAACAG 
GCACTGGACG TGCGCGACGC CTTCGTAAAG GGGATCTACG GGCGGCTGTT CGTGTGGATT 
GTGGACAAGA TCAACGCAGC AATTTACAAG CCTCCCTCCC AGGATGTGAA GAACTCTCGC 
AGGTCCATCG GCCTCCTGGA CATCTTTGGG TTTGAGAACT TTGCTGTGAA CAGCTTTGAG 
CAGCTCTGCA TCAACTTCGC CAATGAGCAC CTGCAGCAGT TCTTTGTGCG GCACGTGTTC 
AAGCTGGAGC AGGAGGAATA TGACCTGGAG AGCATTGACT GGCTGCACAT CGAGTTCACT 
GACAACCAGG ATGCCCTGGA CATGATTGCC AACAAGCCCA TGAACATCAT CTCCCTCATC 
GATGAGGAGA GCAAGTTCCC CAAGGGCACA GACACCACCA TGTTACACAA GCTGAACTCC 
CAGCACAAGC TCAACGCCAA CTACATCCCC CCCAAGAACA ACCATGAGAC CCAGTTTGGC 
ATCAACCATT TTGCAGGCAT CGTCTACTAT GAGACCCAAG GCTTCCTGGA GAAGAACCGA 
GACACCCTGC ATGGGGACAT TATCCAGCTG GTCCACTCCT CCAGGAACAA GTTCATCAAG 
CAGATCTTCC AGGCCGATGT CGCCATGGGC GCCGAGACCA GGAAGCGCTC GCCCACACTT 
AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG CTGATGCGCA CGCTGGGTGC CTGCCAGCCC 
TTCTTTGTGC GATGCATCAA GCCCAATGAG TTCAAGAAGC CCATGCTGTT CGACCGGCAC 
CTGTGCGTGC GCCAGCTGCG GTACTCAGGA ATGATGGAGA CCATCCGAAT CCGCCGAGCT 
GGCTACCCCA TCCGCTACAG CTTCGTAGAG TTTGTGGAGC GGTACCGTGT GCTGCTGCCA 
GGTGTGAAGC CGGCCTACAA GCAGGGCGAC CTCCGCGGGA CTTGCCAGCG CATGGCTGAG 
GCTGTGCTGG GCACCCACGA TGACTGGCAG ATAGGCAAAA CCAAGATCTT TCTGAAGGAC 
CACCATGACA TGCTGCTGGA AGTGGAGCGG GACAAAGCCA TCACCGACAG AGTCATCCTC 
CTTCAGAAAG TCATCCGGGG ATTCAAAGAC AGGTCTAACT TTCTGAAGCT GAAGAACGCT 
GCCACACTGA TCCAGAGGCA CTGGCGGGGT CACAACTGTA GGAAGAACTA CGGGCTGATG 
CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG CACCGCTCCC GGAAGCTGCA CCAGCAGTAC 
CGCCTGGCCC GCCAGCGCAT CATCCAGTTC CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC 
AAGGCCTTCC GCCACCGCCT CTGGGCTGTG CTCACCGTGC AGGCCTATGC CCGGGGCATG 
ATCGCCCGCA GGCTGCACCA ACGCCTCAGG GCTGAGTATC TGTGGCGCCT CGAGGCTGAG 
AAAATGCGGC TGGCGGAGGA AGAGAAGCTT CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG 
GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC CTGGCCCAGC TGGCTCGTGA GGACGCTGAG 
CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG CGGAAGAAGG AGCTCCTGGA GCAGATGGAA 
AGGGCCCGCC ATGAGCCTGT CAATCACTCA GACATGGTGG ACAAGATGTT TGGCTTCCTG 
GGGACTTCAG GTGGCCTGCC AGGCCAGGAG GGCCAGGCAC CTAGTGGCTT TGAGGACCTG 
GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG GACCTGGATG CAGCCCTGCC CCTGCCTGAC 
GAGGATGAGG AGGACCTCTC TGAGTATAAA TTTGCCAAGT TCGCGGCCAC CTACTTCCAG 
GGGACAACCA CGCACTCCTA CACCCGGCGG CCACTCAAAC AGCCACTGCT CTACCATGAC 
GACGAGGGTG ACCAGCTGGC AGCCCTGGCG GTCTGGATCA CCATCCTCCG CTTCATGGGG 
GACCTCCCTG AGCCCAAGTA CCACACAGCC ATGAGTGATG GCAGTGAGAA GATCCCTGTG 
ATGACCAAGA TTTATGAGAC CCTGGGCAAG AAGACGTACA AGAGGGAGCT GCAGGCCCTG 
CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG GGCCAGAAGA AGAGCAGTGT GAGGCACAAG 
CTGGTGCATT TGACTCTGAA AAAGAAGTCC AAGCTCACAG AGGAGGTGAC CAAGAGGCTG 
CATGACGGGG AGTCCACAGT GCAGGGCAAC AGCATGCTGG AGGACCGGCC CACCTCCAAC 
CTGGAGAAGC TGCACTTCAT CATCGGCAAT GGCATCCTGC GGCCAGCACT CCGGGACGAG 
ATCTACTGCC AGATCAGCAA GCAGCTGACC CACAACCCCT CCAAGAGCAG CTATGCCCGG 
GGCTGGATTC TCGTGTCTCT CTGCGTGGGC TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG 
TACCTGCGGA ACTTCATCCA CGGGGGCCCG CCCGGCTACG CCCCGTACTG TGAGGAGCGC 
CTGAGAAGGA CCTTTGTCAA TGGGACACGG ACACAGCCGC CCAGCTGGCT GGAGCTGCAG 
GCCACCAAGT CCAAGAAGCC AATCATGTTG CCCGTGACAT TCATGGATGG GACCACCAAG 
ACCCTGCTGA CGGACTCGGC AACCACGGCC AAGGAGCTCT GCAACGCGCT GGCCGACAAG 
ATCTCTCTCA AGGACCGGTT CGGGTTCTCC CTCTACATTG CCCTGTTTGA CAAGGTGTCC 
TCCCTGGGCA GCGGCAGTGA CCACGTCATG GACGCCATCT CCCAGTGCGA GCAGTACGCC 
AAGGAGCAGG GCGCCCAGGA GCGCAACGCC CCCTGGAGGC TCTTCTTCCG CAAAGAGGTC 
TTCACGCCCT GGCACAGCCC CTCCGAGGAC AACGTGGCCA CCAACCTCAT CTACCAGCAG 
GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC AGGTGTGAGA AGGAGGACGA CCTGGCTGAG 
CTGGCCTCCC AGCAGTACTT TGTAGACTAT GGCTCTGAGA TGATCCTGGA GCGCCTCCTG 
AACCTCGTGC CCACCTACAT CCCCGACCGC GAGATCACGC CCCTGAAGAC GCTGGAGAAG 
TGGGCCCAGC TGGCCATCGC CGCCCACAAG AAGGGGATTT ATGCCCAGAG GAGAACTGAT 
GCCCAGAAGG TCAAAGAGGA TGTGGTCAGT TATGCCCGCT TCAAGTGGCC CTTGCTCTTC 
TCCAGGTTTT ATGAAGCCTA CAAATTCTCA GGCCCCAGTC TCCCCAAGAA CGACGTCATC 
GTGGCCGTCA ACTGGACGGG TGTGTACTTT GTGGATGAGC AGGAGCAGGT ACTTCTGGAG 
CTGTCCTTCC CAGAGATCAT GGCCGTGTCC AGCAGCAGGG GAGCGAAAAC GACGGCCCCC 
AGCTTCACGC TGGCCACCAT CAAGGGGGAC GAATACACCT TCACCTCCAG CAATGCTGAG 
GACATTCGTG ACCTGGTGGT CACCTTCCTA GAGGGGCTCC GGAAGAGATC TAAGTATGTT 
GTGGCCCTGC AGGATAACCC CAACCCCGGC GAGGAGTCAG GCTTCCTCAG CTTTGCCAAG 
GGAGACCTCA TCATCCTGGA CCATGACACG GGCGAGCAGG TCATGAACTC GGGCTGGGCC 
AACGGCATCA ATGAGAGGAC CAAGCAGCGT GGGGACTTCC CCACCGACAG TGTGTACGTC 
ATGCCCACTG TCACCATGCC ACCGCGGGAG ATTGTGGCCC TGGTCACCAT GACTCCCGAT 
CAGAGGCAGG ACGTTGTCCG GCTCTTGCAG CTGCGAACGG CGGAGCCCGA GGTGCGTGCC 
AAGCCCTACA CGCTGGAGGA GTTTTCCTAT GACTACTTCA GGCCCCCACC CAAGCACACG 
CTGAGCCGTG TCATGGTGTC CAAGGCCCGA GGCAAGGACC GGCTGTGGAG CCACACGCGG 
GAACCGCTCA AGCAGGCGCT GCTCAAGAAG CTCCTGGGCA GTGAGGAGCT CTCGCAGGAG 
GCCTGCCTGG CCTTCATTGC TGTGCTCAAG TACATGGGCG ACTACCCGTC CAAGAGGACA 
CGCTCCGTCA ACGAGCTCAC CGACCAGATC TTTGAGGGTC CCCTGAAAGC CGAGCCCCTG 
AAGGACGAGG CATATGTGCA GATCCTGAAG CAGCTGACCG ACAACCACAT CAGGTACAGC 
GAGGAGCGGG GTTGGGAGCT GCTCTGGCTG TGCACGGGCC TTTTCCCACC CAGCAACATC 
CTCCTGCCCC ACGTGCAGCG CTTCCTGCAG TCCCGAAAGC ACTGCCCACT CGCCATCGAC 
TGCCTGCAAC GGCTCCAGAA AGCCCTGAGA AACGGGTCCC GGAAGTACCC TCCGCACCTG 
GTGGAGGTGG AGGCCATCCA GCACAAGACC ACCCAGATTT TCCACAAAGT CTACTTCCCT 
GATGACACTG ACGAGGCCTT CGAAGTGGAG TCCAGCACCA AGGCCAAGGA CTTCTGCCAG 
AACATCGCCA CCAGGCTGCT CCTCAAGTCC TCAGAGGGAT TCAGCCTCTT TGTCAAAATT 
GCAGACAAGG TCCTCAGCGT TCCTGAGAAT GACTTCTTCT TTGACTTTGT TCGACACTTG 
ACAGACTGGA TAAAGAAAGC TCGGCCCATC AAGGACGGAA TTGTGCCCTC ACTCACCTAC 
CAGGTGTTCT TCATGAAGAA GCTGTGGACC ACCACGGTGC CAGGGAAGGA TCCCATGGCC 
GATTCCATCT TCCACTATTA CCAGGAGTTG CCCAAGTATC TCCGAGGCTA CCACAAGTGC 
ACGCGGGAGG AGGTGCTGCA GCTGGGGGCG CTGATCTACA GGGTCAAGTT CGAGGAGGAC 
AAGTCCTACT TCCCCAGCAT CCCCAAGCTG CTGCGGGAGC TGGTGCCCCA GGACCTTATC 
CGGCAGGTCT CACCTGATGA CTGGAAGCGG TCCATCGTCG CCTACTTCAA CAAGCACGCA 
GGGAAGTCCA AGGAGGAGGC CAAGCTGGCC TTCCTGAAGC TCATCTTCAA GTGGCCCACC 
TTTGGCTCAG CCTTCTTCGA GGTGAAGCAA ACTACGGAGC CAAACTTCCC TGAGATCCTC 
CTAATTGCCA TCAACAAGTA TGGGGTCAGC CTCATCGATC CCAAAACGAA GGATATCCTC 
ACCACTCATC CCTTCACCAA GATCTCCAAC TGGAGCAGCG GCAACACCTA CTTCCACATC 
ACCATTGGGA ACTTGGTGCG CGGGAGCAAA CTGCTCTGCG AGACGTCACT GGGCTACAAG 
ATGGATGACC TCCTGACTTC CTACATTAGC CAGATGCTCA CAGCCATGAG CAAACAGCGG 
GGCTCCAGGA GCGGCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO7A ( XM_017017785.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873274.1 Homo sapiens myosin VIIA (MYO7A), transcript variant X10, mRNA.

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CloneID	OHu92262
Clone ID Related Accession (Same CDS sequence)	XM_017017785.1
Accession Version	XM_017017785.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	unconventional myosin-VIIa isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACC CCGCCGACCC TGCCGACCCC GCGCGCATCC CGCAGCCCGG CAAGTGGGCG 
CTGGTGCGCA GCCTGAACCA GGCGTTCAAG ACCTACGCCG TCCTGCCGGG GGACCATGTG 
TGGATGGACC TGAGATTGGG GCAGGAGTTC GACGTGCCCA TCGGGGCGGT GGTGAAGCTC 
TGCGACTCTG GGCAGGTCCA GGTGGTGGAT GATGAAGACA ATGAACACTG GATCTCTCCG 
CAGAACGCAA CGCACATCAA GCCTATGCAC CCCACGTCGG TCCACGGCGT GGAGGACATG 
ATCCGCCTGG GGGACCTCAA CGAGGCGGGC ATCTTGCGCA ACCTGCTTAT CCGCTACCGG 
GACCACCTCA TCTACACGTA TACGGGCTCC ATCCTGGTGG CTGTGAACCC CTACCAGCTG 
CTCTCCATCT ACTCGCCAGA GCACATCCGC CAGTATACCA ACAAGAAGAT TGGGGAGATG 
CCCCCCCACA TCTTTGCCAT TGCTGACAAC TGCTACTTCA ACATGAAACG CAACAGCCGA 
GACCAGTGCT GCATCATCAG TGGGGAATCT GGGGCCGGGA AGACGGAGAG CACAAAGCTG 
ATCCTGCAGT TCCTGGCAGC CATCAGTGGG CAGCACTCGT GGATTGAGCA GCAGGTCTTG 
GAGGCCACCC CCATTCTGGA AGCATTTGGG AATGCCAAGA CCATCCGCAA TGACAACTCA 
AGCCGTTTCG GAAAGTACAT CGACATCCAC TTCAACAAGC GGGGCGCCAT CGAGGGCGCG 
AAGATTGAGC AGTACCTGCT GGAAAAGTCA CGTGTCTGTC GCCAGGCCCT GGATGAAAGG 
AACTACCACG TGTTCTACTG CATGCTGGAG GGTATGAGTG AGGATCAGAA GAAGAAGCTG 
GGCTTGGGCC AGGCCTCTGA CTACAACTAC TTGGCCATGG TGAACCCCCC AGACCTGATG 
AGCTGCCTGA CTAGCCGCAC CCTCATCACC CGCGGGGAGA CGGTGTCCAC CCCACTGAGC 
AGGGAACAGG CACTGGACGT GCGCGACGCC TTCGTAAAGG GGATCTACGG GCGGCTGTTC 
GTGTGGATTG TGGACAAGAT CAACGCAGCA ATTTACAAGC CTCCCTCCCA GGATGTGAAG 
AACTCTCGCA GGTCCATCGG CCTCCTGGAC ATCTTTGGGT TTGAGAACTT TGCTGTGAAC 
AGCTTTGAGC AGCTCTGCAT CAACTTCGCC AATGAGCACC TGCAGCAGTT CTTTGTGCGG 
CACGTGTTCA AGCTGGAGCA GGAGGAATAT GACCTGGAGA GCATTGACTG GCTGCACATC 
GAGTTCACTG ACAACCAGGA TGCCCTGGAC ATGATTGCCA ACAAGCCCAT GAACATCATC 
TCCCTCATCG ATGAGGAGAG CAAGTTCCCC AAGGGCACAG ACACCACCAT GTTACACAAG 
CTGAACTCCC AGCACAAGCT CAACGCCAAC TACATCCCCC CCAAGAACAA CCATGAGACC 
CAGTTTGGCA TCAACCATTT TGCAGGCATC GTCTACTATG AGACCCAAGG CTTCCTGGAG 
AAGAACCGAG ACACCCTGCA TGGGGACATT ATCCAGCTGG TCCACTCCTC CAGGAACAAG 
TTCATCAAGC AGATCTTCCA GGCCGATGTC GCCATGGGCG CCGAGACCAG GAAGCGCTCG 
CCCACACTTA GCAGCCAGTT CAAGCGGTCA CTGGAGCTGC TGATGCGCAC GCTGGGTGCC 
TGCCAGCCCT TCTTTGTGCG ATGCATCAAG CCCAATGAGT TCAAGAAGCC CATGCTGTTC 
GACCGGCACC TGTGCGTGCG CCAGCTGCGG TACTCAGGAA TGATGGAGAC CATCCGAATC 
CGCCGAGCTG GCTACCCCAT CCGCTACAGC TTCGTAGAGT TTGTGGAGCG GTACCGTGTG 
CTGCTGCCAG GTGTGAAGCC GGCCTACAAG CAGGGCGACC TCCGCGGGAC TTGCCAGCGC 
ATGGCTGAGG CTGTGCTGGG CACCCACGAT GACTGGCAGA TAGGCAAAAC CAAGATCTTT 
CTGAAGGACC ACCATGACAT GCTGCTGGAA GTGGAGCGGG ACAAAGCCAT CACCGACAGA 
GTCATCCTCC TTCAGAAAGT CATCCGGGGA TTCAAAGACA GGTCTAACTT TCTGAAGCTG 
AAGAACGCTG CCACACTGAT CCAGAGGCAC TGGCGGGGTC ACAACTGTAG GAAGAACTAC 
GGGCTGATGC GTCTGGGCTT CCTGCGGCTG CAGGCCCTGC ACCGCTCCCG GAAGCTGCAC 
CAGCAGTACC GCCTGGCCCG CCAGCGCATC ATCCAGTTCC AGGCCCGCTG CCGCGCCTAT 
CTGGTGCGCA AGGCCTTCCG CCACCGCCTC TGGGCTGTGC TCACCGTGCA GGCCTATGCC 
CGGGGCATGA TCGCCCGCAG GCTGCACCAA CGCCTCAGGG CTGAGTATCT GTGGCGCCTC 
GAGGCTGAGA AAATGCGGCT GGCGGAGGAA GAGAAGCTTC GGAAGGAGAT GAGCGCCAAG 
AAGGCCAAGG AGGAGGCCGA GCGCAAGCAT CAGGAGCGCC TGGCCCAGCT GGCTCGTGAG 
GACGCTGAGC GGGAGCTGAA GGAGAAGGAG GCCGCTCGGC GGAAGAAGGA GCTCCTGGAG 
CAGATGGAAA GGGCCCGCCA TGAGCCTGTC AATCACTCAG ACATGGTGGA CAAGATGTTT 
GGCTTCCTGG GGACTTCAGG TGGCCTGCCA GGCCAGGAGG GCCAGGCACC TAGTGGCTTT 
GAGGACCTGG AGCGAGGGCG GAGGGAGATG GTGGAGGAGG ACCTGGATGC AGCCCTGCCC 
CTGCCTGACG AGGATGAGGA GGACCTCTCT GAGTATAAAT TTGCCAAGTT CGCGGCCACC 
TACTTCCAGG GGACAACCAC GCACTCCTAC ACCCGGCGGC CACTCAAACA GCCACTGCTC 
TACCATGACG ACGAGGGTGA CCAGCTGGCA GCCCTGGCGG TCTGGATCAC CATCCTCCGC 
TTCATGGGGG ACCTCCCTGA GCCCAAGTAC CACACAGCCA TGAGTGATGG CAGTGAGAAG 
ATCCCTGTGA TGACCAAGAT TTATGAGACC CTGGGCAAGA AGACGTACAA GAGGGAGCTG 
CAGGCCCTGC AGGGCGAGGG CGAGGCCCAG CTCCCCGAGG GCCAGAAGAA GAGCAGTGTG 
AGGCACAAGC TGGTGCATTT GACTCTGAAA AAGAAGTCCA AGCTCACAGA GGAGGTGACC 
AAGAGGCTGC ATGACGGGGA GTCCACAGTG CAGGGCAACA GCATGCTGGA GGACCGGCCC 
ACCTCCAACC TGGAGAAGCT GCACTTCATC ATCGGCAATG GCATCCTGCG GCCAGCACTC 
CGGGACGAGA TCTACTGCCA GATCAGCAAG CAGCTGACCC ACAACCCCTC CAAGAGCAGC 
TATGCCCGGG GCTGGATTCT CGTGTCTCTC TGCGTGGGCT GTTTCGCCCC CTCCGAGAAG 
TTTGTCAAGT ACCTGCGGAA CTTCATCCAC GGGGGCCCGC CCGGCTACGC CCCGTACTGT 
GAGGAGCGCC TGAGAAGGAC CTTTGTCAAT GGGACACGGA CACAGCCGCC CAGCTGGCTG 
GAGCTGCAGG CCACCAAGTC CAAGAAGCCA ATCATGTTGC CCGTGACATT CATGGATGGG 
ACCACCAAGA CCCTGCTGAC GGACTCGGCA ACCACGGCCA AGGAGCTCTG CAACGCGCTG 
GCCGACAAGA TCTCTCTCAA GGACCGGTTC GGGTTCTCCC TCTACATTGC CCTGTTTGAC 
AAGCAACGTC ATGCTCAGCA GAGGGGCCCC CGCCCACAAG CCCAGGGGAG GTACCGTGTT 
GCTGGACACA GTTTGCCTGG ATTTTTCTCA CCGATGCTCT TATGTTTCCA GGTGTCCTCC 
CTGGGCAGCG GCAGTGACCA CGTCATGGAC GCCATCTCCC AGTGCGAGCA GTACGCCAAG 
GAGCAGGGCG CCCAGGAGCG CAACGCCCCC TGGAGGCTCT TCTTCCGCAA AGAGGTCTTC 
ACGCCCTGGC ACAGCCCCTC CGAGGACAAC GTGGCCACCA ACCTCATCTA CCAGCAGGTG 
GTGCGAGGAG TCAAGTTTGG GGAGTACAGG TGTGAGAAGG AGGACGACCT GGCTGAGCTG 
GCCTCCCAGC AGTACTTTGT AGACTATGGC TCTGAGATGA TCCTGGAGCG CCTCCTGAAC 
CTCGTGCCCA CCTACATCCC CGACCGCGAG ATCACGCCCC TGAAGACGCT GGAGAAGTGG 
GCCCAGCTGG CCATCGCCGC CCACAAGAAG GGGATTTATG CCCAGAGGAG AACTGATGCC 
CAGAAGGTCA AAGAGGATGT GGTCAGTTAT GCCCGCTTCA AGTGGCCCTT GCTCTTCTCC 
AGGTTTTATG AAGCCTACAA ATTCTCAGGC CCCAGTCTCC CCAAGAACGA CGTCATCGTG 
GCCGTCAACT GGACGGGTGT GTACTTTGTG GATGAGCAGG AGCAGGTACT TCTGGAGCTG 
TCCTTCCCAG AGATCATGGC CGTGTCCAGC AGCAGGGGAG CGAAAACGAC GGCCCCCAGC 
TTCACGCTGG CCACCATCAA GGGGGACGAA TACACCTTCA CCTCCAGCAA TGCTGAGGAC 
ATTCGTGACC TGGTGGTCAC CTTCCTAGAG GGGCTCCGGA AGAGATCTAA GTATGTTGTG 
GCCCTGCAGG ATAACCCCAA CCCCGCAGGC GAGGAGTCAG GCTTCCTCAG CTTTGCCAAG 
GGAGACCTCA TCATCCTGGA CCATGACACG GGCGAGCAGG TCATGAACTC GGGCTGGGCC 
AACGGCATCA ATGAGAGGAC CAAGCAGCGT GGGGACTTCC CCACCGACAG TGTGTACGTC 
ATGCCCACTG TCACCATGCC ACCGCGGGAG ATTGTGGCCC TGGTCACCAT GACTCCCGAT 
CAGAGGCAGG ACGTTGTCCG GCTCTTGCAG CTGCGAACGG CGGAGCCCGA GGTGCGTGCC 
AAGCCCTACA CGCTGGAGGA GTTTTCCTAT GACTACTTCA GGCCCCCACC CAAGCACACG 
CTGAGCCGTG TCATGGTGTC CAAGGCCCGA GGCAAGGACC GGCTGTGGAG CCACACGCGG 
GAACCGCTCA AGCAGGCGCT GCTCAAGAAG CTCCTGGGCA GTGAGGAGCT CTCGCAGGAG 
GCCTGCCTGG CCTTCATTGC TGTGCTCAAG TACATGGGCG ACTACCCGTC CAAGAGGACA 
CGCTCCGTCA ACGAGCTCAC CGACCAGATC TTTGAGGGTC CCCTGAAAGC CGAGCCCCTG 
AAGGACGAGG CATATGTGCA GATCCTGAAG CAGCTGACCG ACAACCACAT CAGGTACAGC 
GAGGAGCGGG GTTGGGAGCT GCTCTGGCTG TGCACGGGCC TTTTCCCACC CAGCAACATC 
CTCCTGCCCC ACGTGCAGCG CTTCCTGCAG TCCCGAAAGC ACTGCCCACT CGCCATCGAC 
TGCCTGCAAC GGCTCCAGAA AGCCCTGAGA AACGGGTCCC GGAAGTACCC TCCGCACCTG 
GTGGAGGTGG AGGCCATCCA GCACAAGACC ACCCAGATTT TCCACAAAGT CTACTTCCCT 
GATGACACTG ACGAGGCCTT CGAAGTGGAG TCCAGCACCA AGGCCAAGGA CTTCTGCCAG 
AACATCGCCA CCAGGCTGCT CCTCAAGTCC TCAGAGGGAT TCAGCCTCTT TGTCAAAATT 
GCAGACAAGG TCCTCAGCGT TCCTGAGAAT GACTTCTTCT TTGACTTTGT TCGACACTTG 
ACAGACTGGA TAAAGAAAGC TCGGCCCATC AAGGACGGAA TTGTGCCCTC ACTCACCTAC 
CAGGTGTTCT TCATGAAGAA GCTGTGGACC ACCACGGTGC CAGGGAAGGA TCCCATGGCC 
GATTCCATCT TCCACTATTA CCAGGAGTTG CCCAAGTATC TCCGAGGCTA CCACAAGTGC 
ACGCGGGAGG AGGTGCTGCA GCTGGGGGCG CTGATCTACA GGGTCAAGTT CGAGGAGGAC 
AAGTCCTACT TCCCCAGCAT CCCCAAGCTG CTGCGGGAGC TGGTGCCCCA GGACCTTATC 
CGGCAGGTCT CACCTGATGA CTGGAAGCGG TCCATCGTCG CCTACTTCAA CAAGCACGCA 
GGGAAGTCCA AGGAGGAGGC CAAGCTGGCC TTCCTGAAGC TCATCTTCAA GTGGCCCACC 
TTTGGCTCAG CCTTCTTCGA GGTGAAGGTA CACCATGGGC TTCTCAGAGC AGAGGAGGAG 
GGGAACAGGG CATTGATAAA GCACAGAGAC TCCTCCCTAG GACTGTGGGA AAGAGCCATG 
GCCTTGGACA CCTGCCCTGG TGAGGGAAGG AGGGCAGCAC CCTCAGCCTT GTCCCAGCTT 
AGCGTCTTAG CTGGGGTAAG TCCTGGGGAC AGAGGGACCA GAAGAAGCTC AGCCTGGCAG 
TGGAGGCAGA CATGTAAACA GGCTGGGGTA ATGGAGGATG GCCTGTGTTC TGACACTGGG 
GAGCCCTCGC CCAGGTGGCA GAGCTCGGGA GGCTCTGCCG GGTGTGAGAA GGCCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873274.1
CDS	16..6555
Translation	MEDPADPADPARIPQPGKWALVRSLNQAFKTYAVLPGDHVWMDLRLGQEFDVPIGAVVKLCDSGQVQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNSRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQALDERNYHVFYCMLEGMSEDQKKKLGLGQASDYNYLAMVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVDKINAAIYKPPSQDVKNSRRSIGLLDIFGFENFAVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQDALDMIANKPMNIISLIDEESKFPKGTDTTMLHKLNSQHKLNANYIPPKNNHETQFGINHFAGIVYYETQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRRAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLKLKNAATLIQRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLHQQYRLARQRIIQFQARCRAYLVRKAFRHRLWAVLTVQAYARGMIARRLHQRLRAEYLWRLEAEKMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEAARRKKELLEQMERARHEPVNHSDMVDKMFGFLGTSGGLPGQEGQAPSGFEDLERGRREMVEEDLDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGEAQLPEGQKKSSVRHKLVHLTLKKKSKLTEEVTKRLHDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTAKELCNALADKISLKDRFGFSLYIALFDKQRHAQQRGPRPQAQGRYRVAGHSLPGFFSPMLLCFQVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHSPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLNLVPTYIPDREITPLKTLEKWAQLAIAAHKKGIYAQRRTDAQKVKEDVVSYARFKWPLLFSRFYEAYKFSGPSLPKNDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRGAKTTAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDSVYVMPTVTMPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKLLGSEELSQEACLAFIAVLKYMGDYPSKRTRSVNELTDQIFEGPLKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIATRLLLKSSEGFSLFVKIADKVLSVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKVHHGLLRAEEEGNRALIKHRDSSLGLWERAMALDTCPGEGRRAAPSALSQLSVLAGVSPGDRGTRRSSAWQWRQTCKQAGVMEDGLCSDTGEPSPRWQSSGGSAGCEKAS

Target ORF information:

RefSeq Version	XM_017017785.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VIIA (MYO7A), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017785.1

ORF Insert Sequence:

ATGGAGGACC CCGCCGACCC TGCCGACCCC GCGCGCATCC CGCAGCCCGG CAAGTGGGCG 
CTGGTGCGCA GCCTGAACCA GGCGTTCAAG ACCTACGCCG TCCTGCCGGG GGACCATGTG 
TGGATGGACC TGAGATTGGG GCAGGAGTTC GACGTGCCCA TCGGGGCGGT GGTGAAGCTC 
TGCGACTCTG GGCAGGTCCA GGTGGTGGAT GATGAAGACA ATGAACACTG GATCTCTCCG 
CAGAACGCAA CGCACATCAA GCCTATGCAC CCCACGTCGG TCCACGGCGT GGAGGACATG 
ATCCGCCTGG GGGACCTCAA CGAGGCGGGC ATCTTGCGCA ACCTGCTTAT CCGCTACCGG 
GACCACCTCA TCTACACGTA TACGGGCTCC ATCCTGGTGG CTGTGAACCC CTACCAGCTG 
CTCTCCATCT ACTCGCCAGA GCACATCCGC CAGTATACCA ACAAGAAGAT TGGGGAGATG 
CCCCCCCACA TCTTTGCCAT TGCTGACAAC TGCTACTTCA ACATGAAACG CAACAGCCGA 
GACCAGTGCT GCATCATCAG TGGGGAATCT GGGGCCGGGA AGACGGAGAG CACAAAGCTG 
ATCCTGCAGT TCCTGGCAGC CATCAGTGGG CAGCACTCGT GGATTGAGCA GCAGGTCTTG 
GAGGCCACCC CCATTCTGGA AGCATTTGGG AATGCCAAGA CCATCCGCAA TGACAACTCA 
AGCCGTTTCG GAAAGTACAT CGACATCCAC TTCAACAAGC GGGGCGCCAT CGAGGGCGCG 
AAGATTGAGC AGTACCTGCT GGAAAAGTCA CGTGTCTGTC GCCAGGCCCT GGATGAAAGG 
AACTACCACG TGTTCTACTG CATGCTGGAG GGTATGAGTG AGGATCAGAA GAAGAAGCTG 
GGCTTGGGCC AGGCCTCTGA CTACAACTAC TTGGCCATGG TGAACCCCCC AGACCTGATG 
AGCTGCCTGA CTAGCCGCAC CCTCATCACC CGCGGGGAGA CGGTGTCCAC CCCACTGAGC 
AGGGAACAGG CACTGGACGT GCGCGACGCC TTCGTAAAGG GGATCTACGG GCGGCTGTTC 
GTGTGGATTG TGGACAAGAT CAACGCAGCA ATTTACAAGC CTCCCTCCCA GGATGTGAAG 
AACTCTCGCA GGTCCATCGG CCTCCTGGAC ATCTTTGGGT TTGAGAACTT TGCTGTGAAC 
AGCTTTGAGC AGCTCTGCAT CAACTTCGCC AATGAGCACC TGCAGCAGTT CTTTGTGCGG 
CACGTGTTCA AGCTGGAGCA GGAGGAATAT GACCTGGAGA GCATTGACTG GCTGCACATC 
GAGTTCACTG ACAACCAGGA TGCCCTGGAC ATGATTGCCA ACAAGCCCAT GAACATCATC 
TCCCTCATCG ATGAGGAGAG CAAGTTCCCC AAGGGCACAG ACACCACCAT GTTACACAAG 
CTGAACTCCC AGCACAAGCT CAACGCCAAC TACATCCCCC CCAAGAACAA CCATGAGACC 
CAGTTTGGCA TCAACCATTT TGCAGGCATC GTCTACTATG AGACCCAAGG CTTCCTGGAG 
AAGAACCGAG ACACCCTGCA TGGGGACATT ATCCAGCTGG TCCACTCCTC CAGGAACAAG 
TTCATCAAGC AGATCTTCCA GGCCGATGTC GCCATGGGCG CCGAGACCAG GAAGCGCTCG 
CCCACACTTA GCAGCCAGTT CAAGCGGTCA CTGGAGCTGC TGATGCGCAC GCTGGGTGCC 
TGCCAGCCCT TCTTTGTGCG ATGCATCAAG CCCAATGAGT TCAAGAAGCC CATGCTGTTC 
GACCGGCACC TGTGCGTGCG CCAGCTGCGG TACTCAGGAA TGATGGAGAC CATCCGAATC 
CGCCGAGCTG GCTACCCCAT CCGCTACAGC TTCGTAGAGT TTGTGGAGCG GTACCGTGTG 
CTGCTGCCAG GTGTGAAGCC GGCCTACAAG CAGGGCGACC TCCGCGGGAC TTGCCAGCGC 
ATGGCTGAGG CTGTGCTGGG CACCCACGAT GACTGGCAGA TAGGCAAAAC CAAGATCTTT 
CTGAAGGACC ACCATGACAT GCTGCTGGAA GTGGAGCGGG ACAAAGCCAT CACCGACAGA 
GTCATCCTCC TTCAGAAAGT CATCCGGGGA TTCAAAGACA GGTCTAACTT TCTGAAGCTG 
AAGAACGCTG CCACACTGAT CCAGAGGCAC TGGCGGGGTC ACAACTGTAG GAAGAACTAC 
GGGCTGATGC GTCTGGGCTT CCTGCGGCTG CAGGCCCTGC ACCGCTCCCG GAAGCTGCAC 
CAGCAGTACC GCCTGGCCCG CCAGCGCATC ATCCAGTTCC AGGCCCGCTG CCGCGCCTAT 
CTGGTGCGCA AGGCCTTCCG CCACCGCCTC TGGGCTGTGC TCACCGTGCA GGCCTATGCC 
CGGGGCATGA TCGCCCGCAG GCTGCACCAA CGCCTCAGGG CTGAGTATCT GTGGCGCCTC 
GAGGCTGAGA AAATGCGGCT GGCGGAGGAA GAGAAGCTTC GGAAGGAGAT GAGCGCCAAG 
AAGGCCAAGG AGGAGGCCGA GCGCAAGCAT CAGGAGCGCC TGGCCCAGCT GGCTCGTGAG 
GACGCTGAGC GGGAGCTGAA GGAGAAGGAG GCCGCTCGGC GGAAGAAGGA GCTCCTGGAG 
CAGATGGAAA GGGCCCGCCA TGAGCCTGTC AATCACTCAG ACATGGTGGA CAAGATGTTT 
GGCTTCCTGG GGACTTCAGG TGGCCTGCCA GGCCAGGAGG GCCAGGCACC TAGTGGCTTT 
GAGGACCTGG AGCGAGGGCG GAGGGAGATG GTGGAGGAGG ACCTGGATGC AGCCCTGCCC 
CTGCCTGACG AGGATGAGGA GGACCTCTCT GAGTATAAAT TTGCCAAGTT CGCGGCCACC 
TACTTCCAGG GGACAACCAC GCACTCCTAC ACCCGGCGGC CACTCAAACA GCCACTGCTC 
TACCATGACG ACGAGGGTGA CCAGCTGGCA GCCCTGGCGG TCTGGATCAC CATCCTCCGC 
TTCATGGGGG ACCTCCCTGA GCCCAAGTAC CACACAGCCA TGAGTGATGG CAGTGAGAAG 
ATCCCTGTGA TGACCAAGAT TTATGAGACC CTGGGCAAGA AGACGTACAA GAGGGAGCTG 
CAGGCCCTGC AGGGCGAGGG CGAGGCCCAG CTCCCCGAGG GCCAGAAGAA GAGCAGTGTG 
AGGCACAAGC TGGTGCATTT GACTCTGAAA AAGAAGTCCA AGCTCACAGA GGAGGTGACC 
AAGAGGCTGC ATGACGGGGA GTCCACAGTG CAGGGCAACA GCATGCTGGA GGACCGGCCC 
ACCTCCAACC TGGAGAAGCT GCACTTCATC ATCGGCAATG GCATCCTGCG GCCAGCACTC 
CGGGACGAGA TCTACTGCCA GATCAGCAAG CAGCTGACCC ACAACCCCTC CAAGAGCAGC 
TATGCCCGGG GCTGGATTCT CGTGTCTCTC TGCGTGGGCT GTTTCGCCCC CTCCGAGAAG 
TTTGTCAAGT ACCTGCGGAA CTTCATCCAC GGGGGCCCGC CCGGCTACGC CCCGTACTGT 
GAGGAGCGCC TGAGAAGGAC CTTTGTCAAT GGGACACGGA CACAGCCGCC CAGCTGGCTG 
GAGCTGCAGG CCACCAAGTC CAAGAAGCCA ATCATGTTGC CCGTGACATT CATGGATGGG 
ACCACCAAGA CCCTGCTGAC GGACTCGGCA ACCACGGCCA AGGAGCTCTG CAACGCGCTG 
GCCGACAAGA TCTCTCTCAA GGACCGGTTC GGGTTCTCCC TCTACATTGC CCTGTTTGAC 
AAGCAACGTC ATGCTCAGCA GAGGGGCCCC CGCCCACAAG CCCAGGGGAG GTACCGTGTT 
GCTGGACACA GTTTGCCTGG ATTTTTCTCA CCGATGCTCT TATGTTTCCA GGTGTCCTCC 
CTGGGCAGCG GCAGTGACCA CGTCATGGAC GCCATCTCCC AGTGCGAGCA GTACGCCAAG 
GAGCAGGGCG CCCAGGAGCG CAACGCCCCC TGGAGGCTCT TCTTCCGCAA AGAGGTCTTC 
ACGCCCTGGC ACAGCCCCTC CGAGGACAAC GTGGCCACCA ACCTCATCTA CCAGCAGGTG 
GTGCGAGGAG TCAAGTTTGG GGAGTACAGG TGTGAGAAGG AGGACGACCT GGCTGAGCTG 
GCCTCCCAGC AGTACTTTGT AGACTATGGC TCTGAGATGA TCCTGGAGCG CCTCCTGAAC 
CTCGTGCCCA CCTACATCCC CGACCGCGAG ATCACGCCCC TGAAGACGCT GGAGAAGTGG 
GCCCAGCTGG CCATCGCCGC CCACAAGAAG GGGATTTATG CCCAGAGGAG AACTGATGCC 
CAGAAGGTCA AAGAGGATGT GGTCAGTTAT GCCCGCTTCA AGTGGCCCTT GCTCTTCTCC 
AGGTTTTATG AAGCCTACAA ATTCTCAGGC CCCAGTCTCC CCAAGAACGA CGTCATCGTG 
GCCGTCAACT GGACGGGTGT GTACTTTGTG GATGAGCAGG AGCAGGTACT TCTGGAGCTG 
TCCTTCCCAG AGATCATGGC CGTGTCCAGC AGCAGGGGAG CGAAAACGAC GGCCCCCAGC 
TTCACGCTGG CCACCATCAA GGGGGACGAA TACACCTTCA CCTCCAGCAA TGCTGAGGAC 
ATTCGTGACC TGGTGGTCAC CTTCCTAGAG GGGCTCCGGA AGAGATCTAA GTATGTTGTG 
GCCCTGCAGG ATAACCCCAA CCCCGCAGGC GAGGAGTCAG GCTTCCTCAG CTTTGCCAAG 
GGAGACCTCA TCATCCTGGA CCATGACACG GGCGAGCAGG TCATGAACTC GGGCTGGGCC 
AACGGCATCA ATGAGAGGAC CAAGCAGCGT GGGGACTTCC CCACCGACAG TGTGTACGTC 
ATGCCCACTG TCACCATGCC ACCGCGGGAG ATTGTGGCCC TGGTCACCAT GACTCCCGAT 
CAGAGGCAGG ACGTTGTCCG GCTCTTGCAG CTGCGAACGG CGGAGCCCGA GGTGCGTGCC 
AAGCCCTACA CGCTGGAGGA GTTTTCCTAT GACTACTTCA GGCCCCCACC CAAGCACACG 
CTGAGCCGTG TCATGGTGTC CAAGGCCCGA GGCAAGGACC GGCTGTGGAG CCACACGCGG 
GAACCGCTCA AGCAGGCGCT GCTCAAGAAG CTCCTGGGCA GTGAGGAGCT CTCGCAGGAG 
GCCTGCCTGG CCTTCATTGC TGTGCTCAAG TACATGGGCG ACTACCCGTC CAAGAGGACA 
CGCTCCGTCA ACGAGCTCAC CGACCAGATC TTTGAGGGTC CCCTGAAAGC CGAGCCCCTG 
AAGGACGAGG CATATGTGCA GATCCTGAAG CAGCTGACCG ACAACCACAT CAGGTACAGC 
GAGGAGCGGG GTTGGGAGCT GCTCTGGCTG TGCACGGGCC TTTTCCCACC CAGCAACATC 
CTCCTGCCCC ACGTGCAGCG CTTCCTGCAG TCCCGAAAGC ACTGCCCACT CGCCATCGAC 
TGCCTGCAAC GGCTCCAGAA AGCCCTGAGA AACGGGTCCC GGAAGTACCC TCCGCACCTG 
GTGGAGGTGG AGGCCATCCA GCACAAGACC ACCCAGATTT TCCACAAAGT CTACTTCCCT 
GATGACACTG ACGAGGCCTT CGAAGTGGAG TCCAGCACCA AGGCCAAGGA CTTCTGCCAG 
AACATCGCCA CCAGGCTGCT CCTCAAGTCC TCAGAGGGAT TCAGCCTCTT TGTCAAAATT 
GCAGACAAGG TCCTCAGCGT TCCTGAGAAT GACTTCTTCT TTGACTTTGT TCGACACTTG 
ACAGACTGGA TAAAGAAAGC TCGGCCCATC AAGGACGGAA TTGTGCCCTC ACTCACCTAC 
CAGGTGTTCT TCATGAAGAA GCTGTGGACC ACCACGGTGC CAGGGAAGGA TCCCATGGCC 
GATTCCATCT TCCACTATTA CCAGGAGTTG CCCAAGTATC TCCGAGGCTA CCACAAGTGC 
ACGCGGGAGG AGGTGCTGCA GCTGGGGGCG CTGATCTACA GGGTCAAGTT CGAGGAGGAC 
AAGTCCTACT TCCCCAGCAT CCCCAAGCTG CTGCGGGAGC TGGTGCCCCA GGACCTTATC 
CGGCAGGTCT CACCTGATGA CTGGAAGCGG TCCATCGTCG CCTACTTCAA CAAGCACGCA 
GGGAAGTCCA AGGAGGAGGC CAAGCTGGCC TTCCTGAAGC TCATCTTCAA GTGGCCCACC 
TTTGGCTCAG CCTTCTTCGA GGTGAAGGTA CACCATGGGC TTCTCAGAGC AGAGGAGGAG 
GGGAACAGGG CATTGATAAA GCACAGAGAC TCCTCCCTAG GACTGTGGGA AAGAGCCATG 
GCCTTGGACA CCTGCCCTGG TGAGGGAAGG AGGGCAGCAC CCTCAGCCTT GTCCCAGCTT 
AGCGTCTTAG CTGGGGTAAG TCCTGGGGAC AGAGGGACCA GAAGAAGCTC AGCCTGGCAG 
TGGAGGCAGA CATGTAAACA GGCTGGGGTA ATGGAGGATG GCCTGTGTTC TGACACTGGG 
GAGCCCTCGC CCAGGTGGCA GAGCTCGGGA GGCTCTGCCG GGTGTGAGAA GGCCTCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO7A ( XM_017017786.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873275.1 Homo sapiens myosin VIIA (MYO7A), transcript variant X11, mRNA.

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CloneID	OHu92263
Clone ID Related Accession (Same CDS sequence)	XM_017017786.1
Accession Version	XM_017017786.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	unconventional myosin-VIIa isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACC CCGCCGACCC TGCCGACCCC GCGCGCATCC CGCAGCCCGG CAAGTGGGCG 
CTGGTGCGCA GCCTGAACCA GGCGTTCAAG ACCTACGCCG TCCTGCCGGG GGACCATGTG 
TGGATGGACC TGAGATTGGG GCAGGAGTTC GACGTGCCCA TCGGGGCGGT GGTGAAGCTC 
TGCGACTCTG GGCAGGTCCA GGTGGTGGAT GATGAAGACA ATGAACACTG GATCTCTCCG 
CAGAACGCAA CGCACATCAA GCCTATGCAC CCCACGTCGG TCCACGGCGT GGAGGACATG 
ATCCGCCTGG GGGACCTCAA CGAGGCGGGC ATCTTGCGCA ACCTGCTTAT CCGCTACCGG 
GACCACCTCA TCTACACGTA TACGGGCTCC ATCCTGGTGG CTGTGAACCC CTACCAGCTG 
CTCTCCATCT ACTCGCCAGA GCACATCCGC CAGTATACCA ACAAGAAGAT TGGGGAGATG 
CCCCCCCACA TCTTTGCCAT TGCTGACAAC TGCTACTTCA ACATGAAACG CAACAGCCGA 
GACCAGTGCT GCATCATCAG TGGGGAATCT GGGGCCGGGA AGACGGAGAG CACAAAGCTG 
ATCCTGCAGT TCCTGGCAGC CATCAGTGGG CAGCACTCGT GGATTGAGCA GCAGGTCTTG 
GAGGCCACCC CCATTCTGGA AGCATTTGGG AATGCCAAGA CCATCCGCAA TGACAACTCA 
AGCCGTTTCG GAAAGTACAT CGACATCCAC TTCAACAAGC GGGGCGCCAT CGAGGGCGCG 
AAGATTGAGC AGTACCTGCT GGAAAAGTCA CGTGTCTGTC GCCAGGCCCT GGATGAAAGG 
AACTACCACG TGTTCTACTG CATGCTGGAG GGTATGAGTG AGGATCAGAA GAAGAAGCTG 
GGCTTGGGCC AGGCCTCTGA CTACAACTAC TTGGCCATGG GTAACTGCAT AACCTGTGAG 
GGCCGGGTGG ACAGCCAGGA GTACGCCAAC ATCCGCTCCG CCATGAAGGT GCTCATGTTC 
ACTGACACCG AGAACTGGGA GATCTCGAAG CTCCTGGCTG CCATCCTGCA CCTGGGCAAC 
CTGCAGTATG AGGCACGCAC ATTTGAAAAC CTGGATGCCT GTGAGGTTCT CTTCTCCCCA 
TCGCTGGCCA CAGCTGCATC CCTGCTTGAG GTGAACCCCC CAGACCTGAT GAGCTGCCTG 
ACTAGCCGCA CCCTCATCAC CCGCGGGGAG ACGGTGTCCA CCCCACTGAG CAGGGAACAG 
GCACTGGACG TGCGCGACGC CTTCGTAAAG GGGATCTACG GGCGGCTGTT CGTGTGGATT 
GTGGACAAGA TCAACGCAGC AATTTACAAG CCTCCCTCCC AGGATGTGAA GAACTCTCGC 
AGGTCCATCG GCCTCCTGGA CATCTTTGGG TTTGAGAACT TTGCTGTGAA CAGCTTTGAG 
CAGCTCTGCA TCAACTTCGC CAATGAGCAC CTGCAGCAGT TCTTTGTGCG GCACGTGTTC 
AAGCTGGAGC AGGAGGAATA TGACCTGGAG AGCATTGACT GGCTGCACAT CGAGTTCACT 
GACAACCAGG ATGCCCTGGA CATGATTGCC AACAAGCCCA TGAACATCAT CTCCCTCATC 
GATGAGGAGA GCAAGTTCCC CAAGGGCACA GACACCACCA TGTTACACAA GCTGAACTCC 
CAGCACAAGC TCAACGCCAA CTACATCCCC CCCAAGAACA ACCATGAGAC CCAGTTTGGC 
ATCAACCATT TTGCAGGCAT CGTCTACTAT GAGACCCAAG GCTTCCTGGA GAAGAACCGA 
GACACCCTGC ATGGGGACAT TATCCAGCTG GTCCACTCCT CCAGGAACAA GTTCATCAAG 
CAGATCTTCC AGGCCGATGT CGCCATGGGC GCCGAGACCA GGAAGCGCTC GCCCACACTT 
AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG CTGATGCGCA CGCTGGGTGC CTGCCAGCCC 
TTCTTTGTGC GATGCATCAA GCCCAATGAG TTCAAGAAGC CCATGCTGTT CGACCGGCAC 
CTGTGCGTGC GCCAGCTGCG GTACTCAGGA ATGATGGAGA CCATCCGAAT CCGCCGAGCT 
GGCTACCCCA TCCGCTACAG CTTCGTAGAG TTTGTGGAGC GGTACCGTGT GCTGCTGCCA 
GGTGTGAAGC CGGCCTACAA GCAGGGCGAC CTCCGCGGGA CTTGCCAGCG CATGGCTGAG 
GCTGTGCTGG GCACCCACGA TGACTGGCAG ATAGGCAAAA CCAAGATCTT TCTGAAGGAC 
CACCATGACA TGCTGCTGGA AGTGGAGCGG GACAAAGCCA TCACCGACAG AGTCATCCTC 
CTTCAGAAAG TCATCCGGGG ATTCAAAGAC AGGTCTAACT TTCTGAAGCT GAAGAACGCT 
GCCACACTGA TCCAGAGGCA CTGGCGGGGT CACAACTGTA GGAAGAACTA CGGGCTGATG 
CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG CACCGCTCCC GGAAGCTGCA CCAGCAGTAC 
CGCCTGGCCC GCCAGCGCAT CATCCAGTTC CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC 
AAGGCCTTCC GCCACCGCCT CTGGGCTGTG CTCACCGTGC AGGCCTATGC CCGGGGCATG 
ATCGCCCGCA GGCTGCACCA ACGCCTCAGG GCTGAGTATC TGTGGCGCCT CGAGGCTGAG 
AAAATGCGGC TGGCGGAGGA AGAGAAGCTT CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG 
GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC CTGGCCCAGC TGGCTCGTGA GGACGCTGAG 
CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG CGGAAGAAGG AGCTCCTGGA GCAGATGGAA 
AGGGCCCGCC ATGAGCCTGT CAATCACTCA GACATGGTGG ACAAGATGTT TGGCTTCCTG 
GGGACTTCAG GTGGCCTGCC AGGCCAGGAG GGCCAGGCAC CTAGTGGCTT TGAGGACCTG 
GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG GACCTGGATG CAGCCCTGCC CCTGCCTGAC 
GAGGATGAGG AGGACCTCTC TGAGTATAAA TTTGCCAAGT TCGCGGCCAC CTACTTCCAG 
GGGACAACCA CGCACTCCTA CACCCGGCGG CCACTCAAAC AGCCACTGCT CTACCATGAC 
GACGAGGGTG ACCAGCTGGC AGCCCTGGCG GTCTGGATCA CCATCCTCCG CTTCATGGGG 
GACCTCCCTG AGCCCAAGTA CCACACAGCC ATGAGTGATG GCAGTGAGAA GATCCCTGTG 
ATGACCAAGA TTTATGAGAC CCTGGGCAAG AAGACGTACA AGAGGGAGCT GCAGGCCCTG 
CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG GGCCAGAAGA AGAGCAGTGT GAGGCACAAG 
CTGGTGCATT TGACTCTGAA AAAGAAGTCC AAGCTCACAG AGGAGGTGAC CAAGAGGCTG 
CATGACGGGG AGTCCACAGT GCAGGGCAAC AGCATGCTGG AGGACCGGCC CACCTCCAAC 
CTGGAGAAGC TGCACTTCAT CATCGGCAAT GGCATCCTGC GGCCAGCACT CCGGGACGAG 
ATCTACTGCC AGATCAGCAA GCAGCTGACC CACAACCCCT CCAAGAGCAG CTATGCCCGG 
GGCTGGATTC TCGTGTCTCT CTGCGTGGGC TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG 
TACCTGCGGA ACTTCATCCA CGGGGGCCCG CCCGGCTACG CCCCGTACTG TGAGGAGCGC 
CTGAGAAGGA CCTTTGTCAA TGGGACACGG ACACAGCCGC CCAGCTGGCT GGAGCTGCAG 
GCCACCAAGT CCAAGAAGCC AATCATGTTG CCCGTGACAT TCATGGATGG GACCACCAAG 
ACCCTGCTGA CGGACTCGGC AACCACGGCC AAGGAGCTCT GCAACGCGCT GGCCGACAAG 
ATCTCTCTCA AGGACCGGTT CGGGTTCTCC CTCTACATTG CCCTGTTTGA CAAGGTGTCC 
TCCCTGGGCA GCGGCAGTGA CCACGTCATG GACGCCATCT CCCAGTGCGA GCAGTACGCC 
AAGGAGCAGG GCGCCCAGGA GCGCAACGCC CCCTGGAGGC TCTTCTTCCG CAAAGAGGTC 
TTCACGCCCT GGCACAGCCC CTCCGAGGAC AACGTGGCCA CCAACCTCAT CTACCAGCAG 
GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC AGGTGTGAGA AGGAGGACGA CCTGGCTGAG 
CTGGCCTCCC AGCAGTACTT TGTAGACTAT GGCTCTGAGA TGATCCTGGA GCGCCTCCTG 
AACCTCGTGC CCACCTACAT CCCCGACCGC GAGATCACGC CCCTGAAGAC GCTGGAGAAG 
TGGGCCCAGC TGGCCATCGC CGCCCACAAG AAGGGGATTT ATGCCCAGAG GAGAACTGAT 
GCCCAGAAGG TCAAAGAGGA TGTGGTCAGT TATGCCCGCT TCAAGTGGCC CTTGCTCTTC 
TCCAGGTTTT ATGAAGCCTA CAAATTCTCA GGCCCCAGTC TCCCCAAGAA CGACGTCATC 
GTGGCCGTCA ACTGGACGGG TGTGTACTTT GTGGATGAGC AGGAGCAGGT ACTTCTGGAG 
CTGTCCTTCC CAGAGATCAT GGCCGTGTCC AGCAGCAGGG AGTGCCGTGT CTGGCTCTCA 
CTGGGCTGCT CTGATCTTGG CTGTGCTGCG CCTCACTCAG GCTGGGCAGG ACTGACCCCG 
GCGGGGCCCT GTTCTCCGTG TTGGTCCTGC AGGGGAGCGA AAACGACGGC CCCCAGCTTC 
ACGCTGGCCA CCATCAAGGG GGACGAATAC ACCTTCACCT CCAGCAATGC TGAGGACATT 
CGTGACCTGG TGGTCACCTT CCTAGAGGGG CTCCGGAAGA GATCTAAGTA TGTTGTGGCC 
CTGCAGGATA ACCCCAACCC CGCAGGCGAG GAGTCAGGCT TCCTCAGCTT TGCCAAGGGA 
GACCTCATCA TCCTGGACCA TGACACGGGC GAGCAGGTCA TGAACTCGGG CTGGGCCAAC 
GGCATCAATG AGAGGACCAA GCAGCGTGGG GACTTCCCCA CCGACAGTGT GTACGTCATG 
CCCACTGTCA CCATGCCACC GCGGGAGATT GTGGCCCTGG TCACCATGAC TCCCGATCAG 
AGGCAGGACG TTGTCCGGCT CTTGCAGCTG CGAACGGCGG AGCCCGAGGT GCGTGCCAAG 
CCCTACACGC TGGAGGAGTT TTCCTATGAC TACTTCAGGC CCCCACCCAA GCACACGCTG 
AGCCGTGTCA TGGTGTCCAA GGCCCGAGGC AAGGACCGGC TGTGGAGCCA CACGCGGGAA 
CCGCTCAAGC AGGCGCTGCT CAAGAAGCTC CTGGGCAGTG AGGAGCTCTC GCAGGAGGCC 
TGCCTGGCCT TCATTGCTGT GCTCAAGTAC ATGGGCGACT ACCCGTCCAA GAGGACACGC 
TCCGTCAACG AGCTCACCGA CCAGATCTTT GAGGGTCCCC TGAAAGCCGA GCCCCTGAAG 
GACGAGGCAT ATGTGCAGAT CCTGAAGCAG CTGACCGACA ACCACATCAG GTACAGCGAG 
GAGCGGGGTT GGGAGCTGCT CTGGCTGTGC ACGGGCCTTT TCCCACCCAG CAACATCCTC 
CTGCCCCACG TGCAGCGCTT CCTGCAGTCC CGAAAGCACT GCCCACTCGC CATCGACTGC 
CTGCAACGGC TCCAGAAAGC CCTGAGAAAC GGGTCCCGGA AGTACCCTCC GCACCTGGTG 
GAGGTGGAGG CCATCCAGCA CAAGACCACC CAGATTTTCC ACAAAGTCTA CTTCCCTGAT 
GACACTGACG AGGCCTTCGA AGTGGAGTCC AGCACCAAGG CCAAGGACTT CTGCCAGAAC 
ATCGCCACCA GGCTGCTCCT CAAGTCCTCA GAGGGATTCA GCCTCTTTGT CAAAATTGCA 
GACAAGGTCC TCAGCGTTCC TGAGAATGAC TTCTTCTTTG ACTTTGTTCG ACACTTGACA 
GACTGGATAA AGAAAGCTCG GCCCATCAAG GACGGAATTG TGCCCTCACT CACCTACCAG 
GTGTTCTTCA TGAAGAAGCT GTGGACCACC ACGGTGCCAG GGAAGGATCC CATGGCCGAT 
TCCATCTTCC ACTATTACCA GGAGTTGCCC AAGTATCTCC GAGGCTACCA CAAGTGCACG 
CGGGAGGAGG TGCTGCAGCT GGGGGCGCTG ATCTACAGGG TCAAGTTCGA GGAGGACAAG 
TCCTACTTCC CCAGCATCCC CAAGCTGCTG CGGGAGCTGG TGCCCCAGGA CCTTATCCGG 
CAGGTCTCAC CTGATGACTG GAAGCGGTCC ATCGTCGCCT ACTTCAACAA GCACGCAGGG 
AAGTCCAAGG AGGAGGCCAA GCTGGCCTTC CTGAAGCTCA TCTTCAAGTG GCCCACCTTT 
GGCTCAGCCT TCTTCGAGGT GAAGCTAGGA TGGGAGGCCT TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873275.1
CDS	16..6480
Translation	MEDPADPADPARIPQPGKWALVRSLNQAFKTYAVLPGDHVWMDLRLGQEFDVPIGAVVKLCDSGQVQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNSRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQALDERNYHVFYCMLEGMSEDQKKKLGLGQASDYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHLGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVDKINAAIYKPPSQDVKNSRRSIGLLDIFGFENFAVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQDALDMIANKPMNIISLIDEESKFPKGTDTTMLHKLNSQHKLNANYIPPKNNHETQFGINHFAGIVYYETQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRRAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLKLKNAATLIQRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLHQQYRLARQRIIQFQARCRAYLVRKAFRHRLWAVLTVQAYARGMIARRLHQRLRAEYLWRLEAEKMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEAARRKKELLEQMERARHEPVNHSDMVDKMFGFLGTSGGLPGQEGQAPSGFEDLERGRREMVEEDLDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGEAQLPEGQKKSSVRHKLVHLTLKKKSKLTEEVTKRLHDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTAKELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHSPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLNLVPTYIPDREITPLKTLEKWAQLAIAAHKKGIYAQRRTDAQKVKEDVVSYARFKWPLLFSRFYEAYKFSGPSLPKNDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRECRVWLSLGCSDLGCAAPHSGWAGLTPAGPCSPCWSCRGAKTTAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDSVYVMPTVTMPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKLLGSEELSQEACLAFIAVLKYMGDYPSKRTRSVNELTDQIFEGPLKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIATRLLLKSSEGFSLFVKIADKVLSVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKLGWEAF

Target ORF information:

RefSeq Version	XM_017017786.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VIIA (MYO7A), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017786.1

ORF Insert Sequence:

ATGGAGGACC CCGCCGACCC TGCCGACCCC GCGCGCATCC CGCAGCCCGG CAAGTGGGCG 
CTGGTGCGCA GCCTGAACCA GGCGTTCAAG ACCTACGCCG TCCTGCCGGG GGACCATGTG 
TGGATGGACC TGAGATTGGG GCAGGAGTTC GACGTGCCCA TCGGGGCGGT GGTGAAGCTC 
TGCGACTCTG GGCAGGTCCA GGTGGTGGAT GATGAAGACA ATGAACACTG GATCTCTCCG 
CAGAACGCAA CGCACATCAA GCCTATGCAC CCCACGTCGG TCCACGGCGT GGAGGACATG 
ATCCGCCTGG GGGACCTCAA CGAGGCGGGC ATCTTGCGCA ACCTGCTTAT CCGCTACCGG 
GACCACCTCA TCTACACGTA TACGGGCTCC ATCCTGGTGG CTGTGAACCC CTACCAGCTG 
CTCTCCATCT ACTCGCCAGA GCACATCCGC CAGTATACCA ACAAGAAGAT TGGGGAGATG 
CCCCCCCACA TCTTTGCCAT TGCTGACAAC TGCTACTTCA ACATGAAACG CAACAGCCGA 
GACCAGTGCT GCATCATCAG TGGGGAATCT GGGGCCGGGA AGACGGAGAG CACAAAGCTG 
ATCCTGCAGT TCCTGGCAGC CATCAGTGGG CAGCACTCGT GGATTGAGCA GCAGGTCTTG 
GAGGCCACCC CCATTCTGGA AGCATTTGGG AATGCCAAGA CCATCCGCAA TGACAACTCA 
AGCCGTTTCG GAAAGTACAT CGACATCCAC TTCAACAAGC GGGGCGCCAT CGAGGGCGCG 
AAGATTGAGC AGTACCTGCT GGAAAAGTCA CGTGTCTGTC GCCAGGCCCT GGATGAAAGG 
AACTACCACG TGTTCTACTG CATGCTGGAG GGTATGAGTG AGGATCAGAA GAAGAAGCTG 
GGCTTGGGCC AGGCCTCTGA CTACAACTAC TTGGCCATGG GTAACTGCAT AACCTGTGAG 
GGCCGGGTGG ACAGCCAGGA GTACGCCAAC ATCCGCTCCG CCATGAAGGT GCTCATGTTC 
ACTGACACCG AGAACTGGGA GATCTCGAAG CTCCTGGCTG CCATCCTGCA CCTGGGCAAC 
CTGCAGTATG AGGCACGCAC ATTTGAAAAC CTGGATGCCT GTGAGGTTCT CTTCTCCCCA 
TCGCTGGCCA CAGCTGCATC CCTGCTTGAG GTGAACCCCC CAGACCTGAT GAGCTGCCTG 
ACTAGCCGCA CCCTCATCAC CCGCGGGGAG ACGGTGTCCA CCCCACTGAG CAGGGAACAG 
GCACTGGACG TGCGCGACGC CTTCGTAAAG GGGATCTACG GGCGGCTGTT CGTGTGGATT 
GTGGACAAGA TCAACGCAGC AATTTACAAG CCTCCCTCCC AGGATGTGAA GAACTCTCGC 
AGGTCCATCG GCCTCCTGGA CATCTTTGGG TTTGAGAACT TTGCTGTGAA CAGCTTTGAG 
CAGCTCTGCA TCAACTTCGC CAATGAGCAC CTGCAGCAGT TCTTTGTGCG GCACGTGTTC 
AAGCTGGAGC AGGAGGAATA TGACCTGGAG AGCATTGACT GGCTGCACAT CGAGTTCACT 
GACAACCAGG ATGCCCTGGA CATGATTGCC AACAAGCCCA TGAACATCAT CTCCCTCATC 
GATGAGGAGA GCAAGTTCCC CAAGGGCACA GACACCACCA TGTTACACAA GCTGAACTCC 
CAGCACAAGC TCAACGCCAA CTACATCCCC CCCAAGAACA ACCATGAGAC CCAGTTTGGC 
ATCAACCATT TTGCAGGCAT CGTCTACTAT GAGACCCAAG GCTTCCTGGA GAAGAACCGA 
GACACCCTGC ATGGGGACAT TATCCAGCTG GTCCACTCCT CCAGGAACAA GTTCATCAAG 
CAGATCTTCC AGGCCGATGT CGCCATGGGC GCCGAGACCA GGAAGCGCTC GCCCACACTT 
AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG CTGATGCGCA CGCTGGGTGC CTGCCAGCCC 
TTCTTTGTGC GATGCATCAA GCCCAATGAG TTCAAGAAGC CCATGCTGTT CGACCGGCAC 
CTGTGCGTGC GCCAGCTGCG GTACTCAGGA ATGATGGAGA CCATCCGAAT CCGCCGAGCT 
GGCTACCCCA TCCGCTACAG CTTCGTAGAG TTTGTGGAGC GGTACCGTGT GCTGCTGCCA 
GGTGTGAAGC CGGCCTACAA GCAGGGCGAC CTCCGCGGGA CTTGCCAGCG CATGGCTGAG 
GCTGTGCTGG GCACCCACGA TGACTGGCAG ATAGGCAAAA CCAAGATCTT TCTGAAGGAC 
CACCATGACA TGCTGCTGGA AGTGGAGCGG GACAAAGCCA TCACCGACAG AGTCATCCTC 
CTTCAGAAAG TCATCCGGGG ATTCAAAGAC AGGTCTAACT TTCTGAAGCT GAAGAACGCT 
GCCACACTGA TCCAGAGGCA CTGGCGGGGT CACAACTGTA GGAAGAACTA CGGGCTGATG 
CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG CACCGCTCCC GGAAGCTGCA CCAGCAGTAC 
CGCCTGGCCC GCCAGCGCAT CATCCAGTTC CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC 
AAGGCCTTCC GCCACCGCCT CTGGGCTGTG CTCACCGTGC AGGCCTATGC CCGGGGCATG 
ATCGCCCGCA GGCTGCACCA ACGCCTCAGG GCTGAGTATC TGTGGCGCCT CGAGGCTGAG 
AAAATGCGGC TGGCGGAGGA AGAGAAGCTT CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG 
GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC CTGGCCCAGC TGGCTCGTGA GGACGCTGAG 
CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG CGGAAGAAGG AGCTCCTGGA GCAGATGGAA 
AGGGCCCGCC ATGAGCCTGT CAATCACTCA GACATGGTGG ACAAGATGTT TGGCTTCCTG 
GGGACTTCAG GTGGCCTGCC AGGCCAGGAG GGCCAGGCAC CTAGTGGCTT TGAGGACCTG 
GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG GACCTGGATG CAGCCCTGCC CCTGCCTGAC 
GAGGATGAGG AGGACCTCTC TGAGTATAAA TTTGCCAAGT TCGCGGCCAC CTACTTCCAG 
GGGACAACCA CGCACTCCTA CACCCGGCGG CCACTCAAAC AGCCACTGCT CTACCATGAC 
GACGAGGGTG ACCAGCTGGC AGCCCTGGCG GTCTGGATCA CCATCCTCCG CTTCATGGGG 
GACCTCCCTG AGCCCAAGTA CCACACAGCC ATGAGTGATG GCAGTGAGAA GATCCCTGTG 
ATGACCAAGA TTTATGAGAC CCTGGGCAAG AAGACGTACA AGAGGGAGCT GCAGGCCCTG 
CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG GGCCAGAAGA AGAGCAGTGT GAGGCACAAG 
CTGGTGCATT TGACTCTGAA AAAGAAGTCC AAGCTCACAG AGGAGGTGAC CAAGAGGCTG 
CATGACGGGG AGTCCACAGT GCAGGGCAAC AGCATGCTGG AGGACCGGCC CACCTCCAAC 
CTGGAGAAGC TGCACTTCAT CATCGGCAAT GGCATCCTGC GGCCAGCACT CCGGGACGAG 
ATCTACTGCC AGATCAGCAA GCAGCTGACC CACAACCCCT CCAAGAGCAG CTATGCCCGG 
GGCTGGATTC TCGTGTCTCT CTGCGTGGGC TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG 
TACCTGCGGA ACTTCATCCA CGGGGGCCCG CCCGGCTACG CCCCGTACTG TGAGGAGCGC 
CTGAGAAGGA CCTTTGTCAA TGGGACACGG ACACAGCCGC CCAGCTGGCT GGAGCTGCAG 
GCCACCAAGT CCAAGAAGCC AATCATGTTG CCCGTGACAT TCATGGATGG GACCACCAAG 
ACCCTGCTGA CGGACTCGGC AACCACGGCC AAGGAGCTCT GCAACGCGCT GGCCGACAAG 
ATCTCTCTCA AGGACCGGTT CGGGTTCTCC CTCTACATTG CCCTGTTTGA CAAGGTGTCC 
TCCCTGGGCA GCGGCAGTGA CCACGTCATG GACGCCATCT CCCAGTGCGA GCAGTACGCC 
AAGGAGCAGG GCGCCCAGGA GCGCAACGCC CCCTGGAGGC TCTTCTTCCG CAAAGAGGTC 
TTCACGCCCT GGCACAGCCC CTCCGAGGAC AACGTGGCCA CCAACCTCAT CTACCAGCAG 
GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC AGGTGTGAGA AGGAGGACGA CCTGGCTGAG 
CTGGCCTCCC AGCAGTACTT TGTAGACTAT GGCTCTGAGA TGATCCTGGA GCGCCTCCTG 
AACCTCGTGC CCACCTACAT CCCCGACCGC GAGATCACGC CCCTGAAGAC GCTGGAGAAG 
TGGGCCCAGC TGGCCATCGC CGCCCACAAG AAGGGGATTT ATGCCCAGAG GAGAACTGAT 
GCCCAGAAGG TCAAAGAGGA TGTGGTCAGT TATGCCCGCT TCAAGTGGCC CTTGCTCTTC 
TCCAGGTTTT ATGAAGCCTA CAAATTCTCA GGCCCCAGTC TCCCCAAGAA CGACGTCATC 
GTGGCCGTCA ACTGGACGGG TGTGTACTTT GTGGATGAGC AGGAGCAGGT ACTTCTGGAG 
CTGTCCTTCC CAGAGATCAT GGCCGTGTCC AGCAGCAGGG AGTGCCGTGT CTGGCTCTCA 
CTGGGCTGCT CTGATCTTGG CTGTGCTGCG CCTCACTCAG GCTGGGCAGG ACTGACCCCG 
GCGGGGCCCT GTTCTCCGTG TTGGTCCTGC AGGGGAGCGA AAACGACGGC CCCCAGCTTC 
ACGCTGGCCA CCATCAAGGG GGACGAATAC ACCTTCACCT CCAGCAATGC TGAGGACATT 
CGTGACCTGG TGGTCACCTT CCTAGAGGGG CTCCGGAAGA GATCTAAGTA TGTTGTGGCC 
CTGCAGGATA ACCCCAACCC CGCAGGCGAG GAGTCAGGCT TCCTCAGCTT TGCCAAGGGA 
GACCTCATCA TCCTGGACCA TGACACGGGC GAGCAGGTCA TGAACTCGGG CTGGGCCAAC 
GGCATCAATG AGAGGACCAA GCAGCGTGGG GACTTCCCCA CCGACAGTGT GTACGTCATG 
CCCACTGTCA CCATGCCACC GCGGGAGATT GTGGCCCTGG TCACCATGAC TCCCGATCAG 
AGGCAGGACG TTGTCCGGCT CTTGCAGCTG CGAACGGCGG AGCCCGAGGT GCGTGCCAAG 
CCCTACACGC TGGAGGAGTT TTCCTATGAC TACTTCAGGC CCCCACCCAA GCACACGCTG 
AGCCGTGTCA TGGTGTCCAA GGCCCGAGGC AAGGACCGGC TGTGGAGCCA CACGCGGGAA 
CCGCTCAAGC AGGCGCTGCT CAAGAAGCTC CTGGGCAGTG AGGAGCTCTC GCAGGAGGCC 
TGCCTGGCCT TCATTGCTGT GCTCAAGTAC ATGGGCGACT ACCCGTCCAA GAGGACACGC 
TCCGTCAACG AGCTCACCGA CCAGATCTTT GAGGGTCCCC TGAAAGCCGA GCCCCTGAAG 
GACGAGGCAT ATGTGCAGAT CCTGAAGCAG CTGACCGACA ACCACATCAG GTACAGCGAG 
GAGCGGGGTT GGGAGCTGCT CTGGCTGTGC ACGGGCCTTT TCCCACCCAG CAACATCCTC 
CTGCCCCACG TGCAGCGCTT CCTGCAGTCC CGAAAGCACT GCCCACTCGC CATCGACTGC 
CTGCAACGGC TCCAGAAAGC CCTGAGAAAC GGGTCCCGGA AGTACCCTCC GCACCTGGTG 
GAGGTGGAGG CCATCCAGCA CAAGACCACC CAGATTTTCC ACAAAGTCTA CTTCCCTGAT 
GACACTGACG AGGCCTTCGA AGTGGAGTCC AGCACCAAGG CCAAGGACTT CTGCCAGAAC 
ATCGCCACCA GGCTGCTCCT CAAGTCCTCA GAGGGATTCA GCCTCTTTGT CAAAATTGCA 
GACAAGGTCC TCAGCGTTCC TGAGAATGAC TTCTTCTTTG ACTTTGTTCG ACACTTGACA 
GACTGGATAA AGAAAGCTCG GCCCATCAAG GACGGAATTG TGCCCTCACT CACCTACCAG 
GTGTTCTTCA TGAAGAAGCT GTGGACCACC ACGGTGCCAG GGAAGGATCC CATGGCCGAT 
TCCATCTTCC ACTATTACCA GGAGTTGCCC AAGTATCTCC GAGGCTACCA CAAGTGCACG 
CGGGAGGAGG TGCTGCAGCT GGGGGCGCTG ATCTACAGGG TCAAGTTCGA GGAGGACAAG 
TCCTACTTCC CCAGCATCCC CAAGCTGCTG CGGGAGCTGG TGCCCCAGGA CCTTATCCGG 
CAGGTCTCAC CTGATGACTG GAAGCGGTCC ATCGTCGCCT ACTTCAACAA GCACGCAGGG 
AAGTCCAAGG AGGAGGCCAA GCTGGCCTTC CTGAAGCTCA TCTTCAAGTG GCCCACCTTT 
GGCTCAGCCT TCTTCGAGGT GAAGCTAGGA TGGGAGGCCT TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO7A ( XM_017017788.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873277.1 Homo sapiens myosin VIIA (MYO7A), transcript variant X19, mRNA.

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CloneID	OHu92265
Clone ID Related Accession (Same CDS sequence)	XM_017017788.1
Accession Version	XM_017017788.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	unconventional myosin-VIIa isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACC CCGCCGACCC TGCCGACCCC GCGCGCATCC CGCAGCCCGG CAAGTGGGCG 
CTGGTGCGCA GCCTGAACCA GGCGTTCAAG ACCTACGCCG TCCTGCCGGG GGACCATGTG 
TGGATGGACC TGAGATTGGG GCAGGAGTTC GACGTGCCCA TCGGGGCGGT GGTGAAGCTC 
TGCGACTCTG GGCAGGTCCA GGTGGTGGAT GATGAAGACA ATGAACACTG GATCTCTCCG 
CAGAACGCAA CGCACATCAA GCCTATGCAC CCCACGTCGG TCCACGGCGT GGAGGACATG 
ATCCGCCTGG GGGACCTCAA CGAGGCGGGC ATCTTGCGCA ACCTGCTTAT CCGCTACCGG 
GACCACCTCA TCTACACGTA TACGGGCTCC ATCCTGGTGG CTGTGAACCC CTACCAGCTG 
CTCTCCATCT ACTCGCCAGA GCACATCCGC CAGTATACCA ACAAGAAGAT TGGGGAGATG 
CCCCCCCACA TCTTTGCCAT TGCTGACAAC TGCTACTTCA ACATGAAACG CAACAGCCGA 
GACCAGTGCT GCATCATCAG TGGGGAATCT GGGGCCGGGA AGACGGAGAG CACAAAGCTG 
ATCCTGCAGT TCCTGGCAGC CATCAGTGGG CAGCACTCGT GGATTGAGCA GCAGGTCTTG 
GAGGCCACCC CCATTCTGGA AGCATTTGGG AATGCCAAGA CCATCCGCAA TGACAACTCA 
AGCCGTTTCG GAAAGTACAT CGACATCCAC TTCAACAAGC GGGGCGCCAT CGAGGGCGCG 
AAGATTGAGC AGTACCTGCT GGAAAAGTCA CGTGTCTGTC GCCAGGCCCT GGATGAAAGG 
AACTACCACG TGTTCTACTG CATGCTGGAG GGTATGAGTG AGGATCAGAA GAAGAAGCTG 
GGCTTGGGCC AGGCCTCTGA CTACAACTAC TTGGCCATGG GTAACTGCAT AACCTGTGAG 
GGCCGGGTGG ACAGCCAGGA GTACGCCAAC ATCCGCTCCG CCATGAAGGT GCTCATGTTC 
ACTGACACCG AGAACTGGGA GATCTCGAAG CTCCTGGCTG CCATCCTGCA CCTGGGCAAC 
CTGCAGTATG AGGCACGCAC ATTTGAAAAC CTGGATGCCT GTGAGGTTCT CTTCTCCCCA 
TCGCTGGCCA CAGCTGCATC CCTGCTTGAG GTGAACCCCC CAGACCTGAT GAGCTGCCTG 
ACTAGCCGCA CCCTCATCAC CCGCGGGGAG ACGGTGTCCA CCCCACTGAG CAGGGAACAG 
GCACTGGACG TGCGCGACGC CTTCGTAAAG GGGATCTACG GGCGGCTGTT CGTGTGGATT 
GTGGACAAGA TCAACGCAGC AATTTACAAG CCTCCCTCCC AGGATGTGAA GAACTCTCGC 
AGGTCCATCG GCCTCCTGGA CATCTTTGGG TTTGAGAACT TTGCTGTGAA CAGCTTTGAG 
CAGCTCTGCA TCAACTTCGC CAATGAGCAC CTGCAGCAGT TCTTTGTGCG GCACGTGTTC 
AAGCTGGAGC AGGAGGAATA TGACCTGGAG AGCATTGACT GGCTGCACAT CGAGTTCACT 
GACAACCAGG ATGCCCTGGA CATGATTGCC AACAAGCCCA TGAACATCAT CTCCCTCATC 
GATGAGGAGA GCAAGTTCCC CAAGGGCACA GACACCACCA TGTTACACAA GCTGAACTCC 
CAGCACAAGC TCAACGCCAA CTACATCCCC CCCAAGAACA ACCATGAGAC CCAGTTTGGC 
ATCAACCATT TTGCAGGCAT CGTCTACTAT GAGACCCAAG GCTTCCTGGA GAAGAACCGA 
GACACCCTGC ATGGGGACAT TATCCAGCTG GTCCACTCCT CCAGGAACAA GTTCATCAAG 
CAGATCTTCC AGGCCGATGT CGCCATGGGC GCCGAGACCA GGAAGCGCTC GCCCACACTT 
AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG CTGATGCGCA CGCTGGGTGC CTGCCAGCCC 
TTCTTTGTGC GATGCATCAA GCCCAATGAG TTCAAGAAGC CCATGCTGTT CGACCGGCAC 
CTGTGCGTGC GCCAGCTGCG GTACTCAGGA ATGATGGAGA CCATCCGAAT CCGCCGAGCT 
GGCTACCCCA TCCGCTACAG CTTCGTAGAG TTTGTGGAGC GGTACCGTGT GCTGCTGCCA 
GGTGTGAAGC CGGCCTACAA GCAGGGCGAC CTCCGCGGGA CTTGCCAGCG CATGGCTGAG 
GCTGTGCTGG GCACCCACGA TGACTGGCAG ATAGGCAAAA CCAAGATCTT TCTGAAGGAC 
CACCATGACA TGCTGCTGGA AGTGGAGCGG GACAAAGCCA TCACCGACAG AGTCATCCTC 
CTTCAGAAAG TCATCCGGGG ATTCAAAGAC AGGTCTAACT TTCTGAAGCT GAAGAACGCT 
GCCACACTGA TCCAGAGGCA CTGGCGGGGT CACAACTGTA GGAAGAACTA CGGGCTGATG 
CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG CACCGCTCCC GGAAGCTGCA CCAGCAGTAC 
CGCCTGGCCC GCCAGCGCAT CATCCAGTTC CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC 
AAGGCCTTCC GCCACCGCCT CTGGGCTGTG CTCACCGTGC AGGCCTATGC CCGGGGCATG 
ATCGCCCGCA GGCTGCACCA ACGCCTCAGG GCTGAGTATC TGTGGCGCCT CGAGGCTGAG 
AAAATGCGGC TGGCGGAGGA AGAGAAGCTT CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG 
GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC CTGGCCCAGC TGGCTCGTGA GGACGCTGAG 
CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG CGGAAGAAGG AGCTCCTGGA GCAGATGGAA 
AGGGCCCGCC ATGAGCCTGT CAATCACTCA GACATGGTGG ACAAGATGTT TGGCTTCCTG 
GGGACTTCAG GTGGCCTGCC AGGCCAGGAG GGCCAGGCAC CTAGTGGCTT TGAGGACCTG 
GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG GACCTGGATG CAGCCCTGCC CCTGCCTGAC 
GAGGATGAGG AGGACCTCTC TGAGTATAAA TTTGCCAAGT TCGCGGCCAC CTACTTCCAG 
GGGACAACCA CGCACTCCTA CACCCGGCGG CCACTCAAAC AGCCACTGCT CTACCATGAC 
GACGAGGGTG ACCAGCTGGC AGCCCTGGCG GTCTGGATCA CCATCCTCCG CTTCATGGGG 
GACCTCCCTG AGCCCAAGTA CCACACAGCC ATGAGTGATG GCAGTGAGAA GATCCCTGTG 
ATGACCAAGA TTTATGAGAC CCTGGGCAAG AAGACGTACA AGAGGGAGCT GCAGGCCCTG 
CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG GGCCAGAAGA AGAGCAGTGT GAGGCACAAG 
CTGGTGCATT TGACTCTGAA AAAGAAGTCC AAGCTCACAG AGGAGGTGAC CAAGAGGCTG 
CATGACGGGG AGTCCACAGT GCAGGGCAAC AGCATGCTGG AGGACCGGCC CACCTCCAAC 
CTGGAGAAGC TGCACTTCAT CATCGGCAAT GGCATCCTGC GGCCAGCACT CCGGGACGAG 
ATCTACTGCC AGATCAGCAA GCAGCTGACC CACAACCCCT CCAAGAGCAG CTATGCCCGG 
GGCTGGATTC TCGTGTCTCT CTGCGTGGGC TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG 
TACCTGCGGA ACTTCATCCA CGGGGGCCCG CCCGGCTACG CCCCGTACTG TGAGGAGCGC 
CTGAGAAGGA CCTTTGTCAA TGGGACACGG ACACAGCCGC CCAGCTGGCT GGAGCTGCAG 
GCCACCAAGT CCAAGAAGCC AATCATGTTG CCCGTGACAT TCATGGATGG GACCACCAAG 
ACCCTGCTGA CGGACTCGGC AACCACGGCC AAGGAGCTCT GCAACGCGCT GGCCGACAAG 
ATCTCTCTCA AGGACCGGTT CGGGTTCTCC CTCTACATTG CCCTGTTTGA CAAGGTGTCC 
TCCCTGGGCA GCGGCAGTGA CCACGTCATG GACGCCATCT CCCAGTGCGA GCAGTACGCC 
AAGGAGCAGG GCGCCCAGGA GCGCAACGCC CCCTGGAGGC TCTTCTTCCG CAAAGAGGTC 
TTCACGCCCT GGCACAGCCC CTCCGAGGAC AACGTGGCCA CCAACCTCAT CTACCAGCAG 
GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC AGGTGTGAGA AGGAGGACGA CCTGGCTGAG 
CTGGCCTCCC AGCAGTACTT TGTAGACTAT GGCTCTGAGA TGATCCTGGA GCGCCTCCTG 
AACCTCGTGC CCACCTACAT CCCCGACCGC GAGATCACGC CCCTGAAGAC GCTGGAGAAG 
TGGGCCCAGC TGGCCATCGC CGCCCACAAG AAGGGGATTT ATGCCCAGAG GAGAACTGAT 
GCCCAGAAGG TCAAAGAGGA TGTGGTCAGT TATGCCCGCT TCAAGTGGCC CTTGCTCTTC 
TCCAGGTTTT ATGAAGCCTA CAAATTCTCA GGCCCCAGTC TCCCCAAGAA CGACGTCATC 
GTGGCCGTCA ACTGGACGGG TGTGTACTTT GTGGATGAGC AGGAGCAGGT ACTTCTGGAG 
CTGTCCTTCC CAGAGATCAT GGCCGTGTCC AGCAGCAGGG GAGCGAAAAC GACGGCCCCC 
AGCTTCACGC TGGCCACCAT CAAGGGGGAC GAATACACCT TCACCTCCAG CAATGCTGAG 
GACATTCGTG ACCTGGTGGT CACCTTCCTA GAGGGGCTCC GGAAGAGATC TAAGTATGTT 
GTGGCCCTGC AGGATAACCC CAACCCCGCA GGCGAGGAGT CAGGCTTCCT CAGCTTTGCC 
AAGGGAGACC TCATCATCCT GGACCATGAC ACGGGCGAGC AGGTCATGAA CTCGGGCTGG 
GCCAACGGCA TCAATGAGAG GACCAAGCAG CGTGGGGACT TCCCCACCGA CAGTGTGTAC 
GTCATGCCCA CTGTCACCAT GCCACCGCGG GAGATTGTGG CCCTGGTCAC CATGACTCCC 
GATCAGAGGC AGGACGTTGT CCGGCTCTTG CAGCTGCGAA CGGCGGAGCC CGAGGTGCGT 
GCCAAGCCCT ACACGCTGGA GGAGTTTTCC TATGACTACT TCAGGCCCCC ACCCAAGCAC 
ACGCTGAGCC GTGTCATGGT GTCCAAGGCC CGAGGCAAGG ACCGGCTGTG GAGCCACACG 
CGGGAACCGC TCAAGCAGGC GCTGCTCAAG AAGCTCCTGG GCAGTGAGGA GCTCTCGCAG 
GAGGCCTGCC TGGCCTTCAT TGCTGTGCTC AAGTACATGG GCGACTACCC GTCCAAGAGG 
ACACGCTCCG TCAACGAGCT CACCGACCAG ATCTTTGAGG GTCCCCTGAA AGCCGAGCCC 
CTGAAGGACG AGGCATATGT GCAGATCCTG AAGCAGCTGA CCGACAACCA CATCAGGTAC 
AGCGAGGAGC GGGGTTGGGA GCTGCTCTGG CTGTGCACGG GCCTTTTCCC ACCCAGCAAC 
ATCCTCCTGC CCCACGTGCA GCGCTTCCTG CAGTCCCGAA AGCACTGCCC ACTCGCCATC 
GACTGCCTGC AACGGCTCCA GAAAGCCCTG AGAAACGGGT CCCGGAAGTA CCCTCCGCAC 
CTGGTGGAGG TGGAGGCCAT CCAGCACAAG ACCACCCAGA TTTTCCACAA AGTCTACTTC 
CCTGATGACA CTGACGAGGC CTTCGAAGTG GAGTCCAGCA CCAAGGCCAA GGACTTCTGC 
CAGAACATCG CCACCAGGCT GCTCCTCAAG TCCTCAGAGG GATTCAGCCT CTTTGTCAAA 
ATTGCAGACA AGGTCCTCAG CGTTCCTGAG AATGACTTCT TCTTTGACTT TGTTCGACAC 
TTGACAGACT GGATAAAGAA AGCTCGGCCC ATCAAGGACG GAATTGTGCC CTCACTCACC 
TACCAGGTGT TCTTCATGAA GAAGCTGTGG ACCACCACGG TGCCAGGGAA GGATCCCATG 
GCCGATTCCA TCTTCCACTA TTACCAGGAG TTGCCCAAGT ATCTCCGAGG CTACCACAAG 
TGCACGCGGG AGGAGGTGCT GCAGCTGGGG GCGCTGATCT ACAGGGTCAA GTTCGAGGAG 
GACAAGTCCT ACTTCCCCAG CATCCCCAAG CTGCTGCGGG AGCTGGTGCC CCAGGACCTT 
ATCCGGCAGG TCTCACCTGA TGACTGGAAG CGGTCCATCG TCGCCTACTT CAACAAGCAC 
GCAGGGAAGT CCAAGGAGGA GGCCAAGCTG GCCTTCCTGA AGCTCATCTT CAAGTGGCCC 
ACCTTTGGCT CAGCCTTCTT CGAGGTGAAG CAAACTACGG AGCCAAACTT CCCTGAGATC 
CTCCTAATTG CCATCAACAA GTATGGGGTC AGCCTCATCG ATCCCAAAAC GAAGGATATC 
CTCACCACTC ATCCCTTCAC CAAGATCTCC AACTGGAGCA GCGGCAACAC CTACTTCCAC 
ATCACCATTG GGAACTTGGT GCGCGGGAGC AAACTGCTCT GCGAGACGTC ACTGGGCTAC 
AAGATGGATG ACCTCCTGAC TTCCTACATT AGCCAGATGC TCACAGCCAT GAGCAAACAG 
CGGGGCTCCA GGAGCGGCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873277.1
CDS	16..6639
Translation	MEDPADPADPARIPQPGKWALVRSLNQAFKTYAVLPGDHVWMDLRLGQEFDVPIGAVVKLCDSGQVQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNSRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQALDERNYHVFYCMLEGMSEDQKKKLGLGQASDYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHLGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVDKINAAIYKPPSQDVKNSRRSIGLLDIFGFENFAVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQDALDMIANKPMNIISLIDEESKFPKGTDTTMLHKLNSQHKLNANYIPPKNNHETQFGINHFAGIVYYETQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRRAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLKLKNAATLIQRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLHQQYRLARQRIIQFQARCRAYLVRKAFRHRLWAVLTVQAYARGMIARRLHQRLRAEYLWRLEAEKMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEAARRKKELLEQMERARHEPVNHSDMVDKMFGFLGTSGGLPGQEGQAPSGFEDLERGRREMVEEDLDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGEAQLPEGQKKSSVRHKLVHLTLKKKSKLTEEVTKRLHDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTAKELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHSPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLNLVPTYIPDREITPLKTLEKWAQLAIAAHKKGIYAQRRTDAQKVKEDVVSYARFKWPLLFSRFYEAYKFSGPSLPKNDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRGAKTTAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDSVYVMPTVTMPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKLLGSEELSQEACLAFIAVLKYMGDYPSKRTRSVNELTDQIFEGPLKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIATRLLLKSSEGFSLFVKIADKVLSVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKQTTEPNFPEILLIAINKYGVSLIDPKTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRGSRSGK

Target ORF information:

RefSeq Version	XM_017017788.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VIIA (MYO7A), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017788.1

ORF Insert Sequence:

ATGGAGGACC CCGCCGACCC TGCCGACCCC GCGCGCATCC CGCAGCCCGG CAAGTGGGCG 
CTGGTGCGCA GCCTGAACCA GGCGTTCAAG ACCTACGCCG TCCTGCCGGG GGACCATGTG 
TGGATGGACC TGAGATTGGG GCAGGAGTTC GACGTGCCCA TCGGGGCGGT GGTGAAGCTC 
TGCGACTCTG GGCAGGTCCA GGTGGTGGAT GATGAAGACA ATGAACACTG GATCTCTCCG 
CAGAACGCAA CGCACATCAA GCCTATGCAC CCCACGTCGG TCCACGGCGT GGAGGACATG 
ATCCGCCTGG GGGACCTCAA CGAGGCGGGC ATCTTGCGCA ACCTGCTTAT CCGCTACCGG 
GACCACCTCA TCTACACGTA TACGGGCTCC ATCCTGGTGG CTGTGAACCC CTACCAGCTG 
CTCTCCATCT ACTCGCCAGA GCACATCCGC CAGTATACCA ACAAGAAGAT TGGGGAGATG 
CCCCCCCACA TCTTTGCCAT TGCTGACAAC TGCTACTTCA ACATGAAACG CAACAGCCGA 
GACCAGTGCT GCATCATCAG TGGGGAATCT GGGGCCGGGA AGACGGAGAG CACAAAGCTG 
ATCCTGCAGT TCCTGGCAGC CATCAGTGGG CAGCACTCGT GGATTGAGCA GCAGGTCTTG 
GAGGCCACCC CCATTCTGGA AGCATTTGGG AATGCCAAGA CCATCCGCAA TGACAACTCA 
AGCCGTTTCG GAAAGTACAT CGACATCCAC TTCAACAAGC GGGGCGCCAT CGAGGGCGCG 
AAGATTGAGC AGTACCTGCT GGAAAAGTCA CGTGTCTGTC GCCAGGCCCT GGATGAAAGG 
AACTACCACG TGTTCTACTG CATGCTGGAG GGTATGAGTG AGGATCAGAA GAAGAAGCTG 
GGCTTGGGCC AGGCCTCTGA CTACAACTAC TTGGCCATGG GTAACTGCAT AACCTGTGAG 
GGCCGGGTGG ACAGCCAGGA GTACGCCAAC ATCCGCTCCG CCATGAAGGT GCTCATGTTC 
ACTGACACCG AGAACTGGGA GATCTCGAAG CTCCTGGCTG CCATCCTGCA CCTGGGCAAC 
CTGCAGTATG AGGCACGCAC ATTTGAAAAC CTGGATGCCT GTGAGGTTCT CTTCTCCCCA 
TCGCTGGCCA CAGCTGCATC CCTGCTTGAG GTGAACCCCC CAGACCTGAT GAGCTGCCTG 
ACTAGCCGCA CCCTCATCAC CCGCGGGGAG ACGGTGTCCA CCCCACTGAG CAGGGAACAG 
GCACTGGACG TGCGCGACGC CTTCGTAAAG GGGATCTACG GGCGGCTGTT CGTGTGGATT 
GTGGACAAGA TCAACGCAGC AATTTACAAG CCTCCCTCCC AGGATGTGAA GAACTCTCGC 
AGGTCCATCG GCCTCCTGGA CATCTTTGGG TTTGAGAACT TTGCTGTGAA CAGCTTTGAG 
CAGCTCTGCA TCAACTTCGC CAATGAGCAC CTGCAGCAGT TCTTTGTGCG GCACGTGTTC 
AAGCTGGAGC AGGAGGAATA TGACCTGGAG AGCATTGACT GGCTGCACAT CGAGTTCACT 
GACAACCAGG ATGCCCTGGA CATGATTGCC AACAAGCCCA TGAACATCAT CTCCCTCATC 
GATGAGGAGA GCAAGTTCCC CAAGGGCACA GACACCACCA TGTTACACAA GCTGAACTCC 
CAGCACAAGC TCAACGCCAA CTACATCCCC CCCAAGAACA ACCATGAGAC CCAGTTTGGC 
ATCAACCATT TTGCAGGCAT CGTCTACTAT GAGACCCAAG GCTTCCTGGA GAAGAACCGA 
GACACCCTGC ATGGGGACAT TATCCAGCTG GTCCACTCCT CCAGGAACAA GTTCATCAAG 
CAGATCTTCC AGGCCGATGT CGCCATGGGC GCCGAGACCA GGAAGCGCTC GCCCACACTT 
AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG CTGATGCGCA CGCTGGGTGC CTGCCAGCCC 
TTCTTTGTGC GATGCATCAA GCCCAATGAG TTCAAGAAGC CCATGCTGTT CGACCGGCAC 
CTGTGCGTGC GCCAGCTGCG GTACTCAGGA ATGATGGAGA CCATCCGAAT CCGCCGAGCT 
GGCTACCCCA TCCGCTACAG CTTCGTAGAG TTTGTGGAGC GGTACCGTGT GCTGCTGCCA 
GGTGTGAAGC CGGCCTACAA GCAGGGCGAC CTCCGCGGGA CTTGCCAGCG CATGGCTGAG 
GCTGTGCTGG GCACCCACGA TGACTGGCAG ATAGGCAAAA CCAAGATCTT TCTGAAGGAC 
CACCATGACA TGCTGCTGGA AGTGGAGCGG GACAAAGCCA TCACCGACAG AGTCATCCTC 
CTTCAGAAAG TCATCCGGGG ATTCAAAGAC AGGTCTAACT TTCTGAAGCT GAAGAACGCT 
GCCACACTGA TCCAGAGGCA CTGGCGGGGT CACAACTGTA GGAAGAACTA CGGGCTGATG 
CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG CACCGCTCCC GGAAGCTGCA CCAGCAGTAC 
CGCCTGGCCC GCCAGCGCAT CATCCAGTTC CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC 
AAGGCCTTCC GCCACCGCCT CTGGGCTGTG CTCACCGTGC AGGCCTATGC CCGGGGCATG 
ATCGCCCGCA GGCTGCACCA ACGCCTCAGG GCTGAGTATC TGTGGCGCCT CGAGGCTGAG 
AAAATGCGGC TGGCGGAGGA AGAGAAGCTT CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG 
GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC CTGGCCCAGC TGGCTCGTGA GGACGCTGAG 
CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG CGGAAGAAGG AGCTCCTGGA GCAGATGGAA 
AGGGCCCGCC ATGAGCCTGT CAATCACTCA GACATGGTGG ACAAGATGTT TGGCTTCCTG 
GGGACTTCAG GTGGCCTGCC AGGCCAGGAG GGCCAGGCAC CTAGTGGCTT TGAGGACCTG 
GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG GACCTGGATG CAGCCCTGCC CCTGCCTGAC 
GAGGATGAGG AGGACCTCTC TGAGTATAAA TTTGCCAAGT TCGCGGCCAC CTACTTCCAG 
GGGACAACCA CGCACTCCTA CACCCGGCGG CCACTCAAAC AGCCACTGCT CTACCATGAC 
GACGAGGGTG ACCAGCTGGC AGCCCTGGCG GTCTGGATCA CCATCCTCCG CTTCATGGGG 
GACCTCCCTG AGCCCAAGTA CCACACAGCC ATGAGTGATG GCAGTGAGAA GATCCCTGTG 
ATGACCAAGA TTTATGAGAC CCTGGGCAAG AAGACGTACA AGAGGGAGCT GCAGGCCCTG 
CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG GGCCAGAAGA AGAGCAGTGT GAGGCACAAG 
CTGGTGCATT TGACTCTGAA AAAGAAGTCC AAGCTCACAG AGGAGGTGAC CAAGAGGCTG 
CATGACGGGG AGTCCACAGT GCAGGGCAAC AGCATGCTGG AGGACCGGCC CACCTCCAAC 
CTGGAGAAGC TGCACTTCAT CATCGGCAAT GGCATCCTGC GGCCAGCACT CCGGGACGAG 
ATCTACTGCC AGATCAGCAA GCAGCTGACC CACAACCCCT CCAAGAGCAG CTATGCCCGG 
GGCTGGATTC TCGTGTCTCT CTGCGTGGGC TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG 
TACCTGCGGA ACTTCATCCA CGGGGGCCCG CCCGGCTACG CCCCGTACTG TGAGGAGCGC 
CTGAGAAGGA CCTTTGTCAA TGGGACACGG ACACAGCCGC CCAGCTGGCT GGAGCTGCAG 
GCCACCAAGT CCAAGAAGCC AATCATGTTG CCCGTGACAT TCATGGATGG GACCACCAAG 
ACCCTGCTGA CGGACTCGGC AACCACGGCC AAGGAGCTCT GCAACGCGCT GGCCGACAAG 
ATCTCTCTCA AGGACCGGTT CGGGTTCTCC CTCTACATTG CCCTGTTTGA CAAGGTGTCC 
TCCCTGGGCA GCGGCAGTGA CCACGTCATG GACGCCATCT CCCAGTGCGA GCAGTACGCC 
AAGGAGCAGG GCGCCCAGGA GCGCAACGCC CCCTGGAGGC TCTTCTTCCG CAAAGAGGTC 
TTCACGCCCT GGCACAGCCC CTCCGAGGAC AACGTGGCCA CCAACCTCAT CTACCAGCAG 
GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC AGGTGTGAGA AGGAGGACGA CCTGGCTGAG 
CTGGCCTCCC AGCAGTACTT TGTAGACTAT GGCTCTGAGA TGATCCTGGA GCGCCTCCTG 
AACCTCGTGC CCACCTACAT CCCCGACCGC GAGATCACGC CCCTGAAGAC GCTGGAGAAG 
TGGGCCCAGC TGGCCATCGC CGCCCACAAG AAGGGGATTT ATGCCCAGAG GAGAACTGAT 
GCCCAGAAGG TCAAAGAGGA TGTGGTCAGT TATGCCCGCT TCAAGTGGCC CTTGCTCTTC 
TCCAGGTTTT ATGAAGCCTA CAAATTCTCA GGCCCCAGTC TCCCCAAGAA CGACGTCATC 
GTGGCCGTCA ACTGGACGGG TGTGTACTTT GTGGATGAGC AGGAGCAGGT ACTTCTGGAG 
CTGTCCTTCC CAGAGATCAT GGCCGTGTCC AGCAGCAGGG GAGCGAAAAC GACGGCCCCC 
AGCTTCACGC TGGCCACCAT CAAGGGGGAC GAATACACCT TCACCTCCAG CAATGCTGAG 
GACATTCGTG ACCTGGTGGT CACCTTCCTA GAGGGGCTCC GGAAGAGATC TAAGTATGTT 
GTGGCCCTGC AGGATAACCC CAACCCCGCA GGCGAGGAGT CAGGCTTCCT CAGCTTTGCC 
AAGGGAGACC TCATCATCCT GGACCATGAC ACGGGCGAGC AGGTCATGAA CTCGGGCTGG 
GCCAACGGCA TCAATGAGAG GACCAAGCAG CGTGGGGACT TCCCCACCGA CAGTGTGTAC 
GTCATGCCCA CTGTCACCAT GCCACCGCGG GAGATTGTGG CCCTGGTCAC CATGACTCCC 
GATCAGAGGC AGGACGTTGT CCGGCTCTTG CAGCTGCGAA CGGCGGAGCC CGAGGTGCGT 
GCCAAGCCCT ACACGCTGGA GGAGTTTTCC TATGACTACT TCAGGCCCCC ACCCAAGCAC 
ACGCTGAGCC GTGTCATGGT GTCCAAGGCC CGAGGCAAGG ACCGGCTGTG GAGCCACACG 
CGGGAACCGC TCAAGCAGGC GCTGCTCAAG AAGCTCCTGG GCAGTGAGGA GCTCTCGCAG 
GAGGCCTGCC TGGCCTTCAT TGCTGTGCTC AAGTACATGG GCGACTACCC GTCCAAGAGG 
ACACGCTCCG TCAACGAGCT CACCGACCAG ATCTTTGAGG GTCCCCTGAA AGCCGAGCCC 
CTGAAGGACG AGGCATATGT GCAGATCCTG AAGCAGCTGA CCGACAACCA CATCAGGTAC 
AGCGAGGAGC GGGGTTGGGA GCTGCTCTGG CTGTGCACGG GCCTTTTCCC ACCCAGCAAC 
ATCCTCCTGC CCCACGTGCA GCGCTTCCTG CAGTCCCGAA AGCACTGCCC ACTCGCCATC 
GACTGCCTGC AACGGCTCCA GAAAGCCCTG AGAAACGGGT CCCGGAAGTA CCCTCCGCAC 
CTGGTGGAGG TGGAGGCCAT CCAGCACAAG ACCACCCAGA TTTTCCACAA AGTCTACTTC 
CCTGATGACA CTGACGAGGC CTTCGAAGTG GAGTCCAGCA CCAAGGCCAA GGACTTCTGC 
CAGAACATCG CCACCAGGCT GCTCCTCAAG TCCTCAGAGG GATTCAGCCT CTTTGTCAAA 
ATTGCAGACA AGGTCCTCAG CGTTCCTGAG AATGACTTCT TCTTTGACTT TGTTCGACAC 
TTGACAGACT GGATAAAGAA AGCTCGGCCC ATCAAGGACG GAATTGTGCC CTCACTCACC 
TACCAGGTGT TCTTCATGAA GAAGCTGTGG ACCACCACGG TGCCAGGGAA GGATCCCATG 
GCCGATTCCA TCTTCCACTA TTACCAGGAG TTGCCCAAGT ATCTCCGAGG CTACCACAAG 
TGCACGCGGG AGGAGGTGCT GCAGCTGGGG GCGCTGATCT ACAGGGTCAA GTTCGAGGAG 
GACAAGTCCT ACTTCCCCAG CATCCCCAAG CTGCTGCGGG AGCTGGTGCC CCAGGACCTT 
ATCCGGCAGG TCTCACCTGA TGACTGGAAG CGGTCCATCG TCGCCTACTT CAACAAGCAC 
GCAGGGAAGT CCAAGGAGGA GGCCAAGCTG GCCTTCCTGA AGCTCATCTT CAAGTGGCCC 
ACCTTTGGCT CAGCCTTCTT CGAGGTGAAG CAAACTACGG AGCCAAACTT CCCTGAGATC 
CTCCTAATTG CCATCAACAA GTATGGGGTC AGCCTCATCG ATCCCAAAAC GAAGGATATC 
CTCACCACTC ATCCCTTCAC CAAGATCTCC AACTGGAGCA GCGGCAACAC CTACTTCCAC 
ATCACCATTG GGAACTTGGT GCGCGGGAGC AAACTGCTCT GCGAGACGTC ACTGGGCTAC 
AAGATGGATG ACCTCCTGAC TTCCTACATT AGCCAGATGC TCACAGCCAT GAGCAAACAG 
CGGGGCTCCA GGAGCGGCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO7A ( NM_001127180.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001120652.1 Homo sapiens myosin VIIA (MYO7A), transcript variant 2, mRNA.

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CloneID	OHu26178
Clone ID Related Accession (Same CDS sequence)	NM_001127180.1 , NM_001127180.2
Accession Version	NM_001127180.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Homo sapiens(human)
Product	unconventional myosin-VIIa isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U55208.1, U39226.1 and BG108466.1. Transcript Variant: This variant (2) lacks an in-frame segment in the coding region, compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U55208.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGATTC TTCAGCAGGG GGACCATGTG TGGATGGACC TGAGATTGGG GCAGGAGTTC 
GACGTGCCCA TCGGGGCGGT GGTGAAGCTC TGCGACTCTG GGCAGGTCCA GGTGGTGGAT 
GATGAAGACA ATGAACACTG GATCTCTCCG CAGAACGCAA CGCACATCAA GCCTATGCAC 
CCCACGTCGG TCCACGGCGT GGAGGACATG ATCCGCCTGG GGGACCTCAA CGAGGCGGGC 
ATCTTGCGCA ACCTGCTTAT CCGCTACCGG GACCACCTCA TCTACACGTA TACGGGCTCC 
ATCCTGGTGG CTGTGAACCC CTACCAGCTG CTCTCCATCT ACTCGCCAGA GCACATCCGC 
CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAC 
TGCTACTTCA ACATGAAACG CAACAGCCGA GACCAGTGCT GCATCATCAG TGGGGAATCT 
GGGGCCGGGA AGACGGAGAG CACAAAGCTG ATCCTGCAGT TCCTGGCAGC CATCAGTGGG 
CAGCACTCGT GGATTGAGCA GCAGGTCTTG GAGGCCACCC CCATTCTGGA AGCATTTGGG 
AATGCCAAGA CCATCCGCAA TGACAACTCA AGCCGTTTCG GAAAGTACAT CGACATCCAC 
TTCAACAAGC GGGGCGCCAT CGAGGGCGCG AAGATTGAGC AGTACCTGCT GGAAAAGTCA 
CGTGTCTGTC GCCAGGCCCT GGATGAAAGG AACTACCACG TGTTCTACTG CATGCTGGAG 
GGTATGAGTG AGGATCAGAA GAAGAAGCTG GGCTTGGGCC AGGCCTCTGA CTACAACTAC 
TTGGCCATGG GTAACTGCAT AACCTGTGAG GGCCGGGTGG ACAGCCAGGA GTACGCCAAC 
ATCCGCTCCG CCATGAAGGT GCTCATGTTC ACTGACACCG AGAACTGGGA GATCTCGAAG 
CTCCTGGCTG CCATCCTGCA CCTGGGCAAC CTGCAGTATG AGGCACGCAC ATTTGAAAAC 
CTGGATGCCT GTGAGGTTCT CTTCTCCCCA TCGCTGGCCA CAGCTGCATC CCTGCTTGAG 
GTGAACCCCC CAGACCTGAT GAGCTGCCTG ACTAGCCGCA CCCTCATCAC CCGCGGGGAG 
ACGGTGTCCA CCCCACTGAG CAGGGAACAG GCACTGGACG TGCGCGACGC CTTCGTAAAG 
GGGATCTACG GGCGGCTGTT CGTGTGGATT GTGGACAAGA TCAACGCAGC AATTTACAAG 
CCTCCCTCCC AGGATGTGAA GAACTCTCGC AGGTCCATCG GCCTCCTGGA CATCTTTGGG 
TTTGAGAACT TTGCTGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTCGC CAATGAGCAC 
CTGCAGCAGT TCTTTGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAATA TGACCTGGAG 
AGCATTGACT GGCTGCACAT CGAGTTCACT GACAACCAGG ATGCCCTGGA CATGATTGCC 
AACAAGCCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACA 
GACACCACCA TGTTACACAA GCTGAACTCC CAGCACAAGC TCAACGCCAA CTACATCCCC 
CCCAAGAACA ACCATGAGAC CCAGTTTGGC ATCAACCATT TTGCAGGCAT CGTCTACTAT 
GAGACCCAAG GCTTCCTGGA GAAGAACCGA GACACCCTGC ATGGGGACAT TATCCAGCTG 
GTCCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT CGCCATGGGC 
GCCGAGACCA GGAAGCGCTC GCCCACACTT AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG 
CTGATGCGCA CGCTGGGTGC CTGCCAGCCC TTCTTTGTGC GATGCATCAA GCCCAATGAG 
TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGCGTGC GCCAGCTGCG GTACTCAGGA 
ATGATGGAGA CCATCCGAAT CCGCCGAGCT GGCTACCCCA TCCGCTACAG CTTCGTAGAG 
TTTGTGGAGC GGTACCGTGT GCTGCTGCCA GGTGTGAAGC CGGCCTACAA GCAGGGCGAC 
CTCCGCGGGA CTTGCCAGCG CATGGCTGAG GCTGTGCTGG GCACCCACGA TGACTGGCAG 
ATAGGCAAAA CCAAGATCTT TCTGAAGGAC CACCATGACA TGCTGCTGGA AGTGGAGCGG 
GACAAAGCCA TCACCGACAG AGTCATCCTC CTTCAGAAAG TCATCCGGGG ATTCAAAGAC 
AGGTCTAACT TTCTGAAGCT GAAGAACGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGT 
CACAACTGTA GGAAGAACTA CGGGCTGATG CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG 
CACCGCTCCC GGAAGCTGCA CCAGCAGTAC CGCCTGGCCC GCCAGCGCAT CATCCAGTTC 
CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC AAGGCCTTCC GCCACCGCCT CTGGGCTGTG 
CTCACCGTGC AGGCCTATGC CCGGGGCATG ATCGCCCGCA GGCTGCACCA ACGCCTCAGG 
GCTGAGTATC TGTGGCGCCT CGAGGCTGAG AAAATGCGGC TGGCGGAGGA AGAGAAGCTT 
CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC 
CTGGCCCAGC TGGCTCGTGA GGACGCTGAG CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG 
CGGAAGAAGG AGCTCCTGGA GCAGATGGAA AGGGCCCGCC ATGAGCCTGT CAATCACTCA 
GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCAG GTGGCCTGCC AGGCCAGGAG 
GGCCAGGCAC CTAGTGGCTT TGAGGACCTG GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG 
GACCTGGATG CAGCCCTGCC CCTGCCTGAC GAGGATGAGG AGGACCTCTC TGAGTATAAA 
TTTGCCAAGT TCGCGGCCAC CTACTTCCAG GGGACAACCA CGCACTCCTA CACCCGGCGG 
CCACTCAAAC AGCCACTGCT CTACCATGAC GACGAGGGTG ACCAGCTGGC AGCCCTGGCG 
GTCTGGATCA CCATCCTCCG CTTCATGGGG GACCTCCCTG AGCCCAAGTA CCACACAGCC 
ATGAGTGATG GCAGTGAGAA GATCCCTGTG ATGACCAAGA TTTATGAGAC CCTGGGCAAG 
AAGACGTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG 
GGCCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCATT TGACTCTGAA AAAGAAGTCC 
AAGCTCACAG AGGAGGTGAC CAAGAGGCTG CATGACGGGG AGTCCACAGT GCAGGGCAAC 
AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATCGGCAAT 
GGCATCCTGC GGCCAGCACT CCGGGACGAG ATCTACTGCC AGATCAGCAA GCAGCTGACC 
CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATTC TCGTGTCTCT CTGCGTGGGC 
TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG TACCTGCGGA ACTTCATCCA CGGGGGCCCG 
CCCGGCTACG CCCCGTACTG TGAGGAGCGC CTGAGAAGGA CCTTTGTCAA TGGGACACGG 
ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC AATCATGTTG 
CCCGTGACAT TCATGGATGG GACCACCAAG ACCCTGCTGA CGGACTCGGC AACCACGGCC 
AAGGAGCTCT GCAACGCGCT GGCCGACAAG ATCTCTCTCA AGGACCGGTT CGGGTTCTCC 
CTCTACATTG CCCTGTTTGA CAAGGTGTCC TCCCTGGGCA GCGGCAGTGA CCACGTCATG 
GACGCCATCT CCCAGTGCGA GCAGTACGCC AAGGAGCAGG GCGCCCAGGA GCGCAACGCC 
CCCTGGAGGC TCTTCTTCCG CAAAGAGGTC TTCACGCCCT GGCACAGCCC CTCCGAGGAC 
AACGTGGCCA CCAACCTCAT CTACCAGCAG GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC 
AGGTGTGAGA AGGAGGACGA CCTGGCTGAG CTGGCCTCCC AGCAGTACTT TGTAGACTAT 
GGCTCTGAGA TGATCCTGGA GCGCCTCCTG AACCTCGTGC CCACCTACAT CCCCGACCGC 
GAGATCACGC CCCTGAAGAC GCTGGAGAAG TGGGCCCAGC TGGCCATCGC CGCCCACAAG 
AAGGGGATTT ATGCCCAGAG GAGAACTGAT GCCCAGAAGG TCAAAGAGGA TGTGGTCAGT 
TATGCCCGCT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ATGAAGCCTA CAAATTCTCA 
GGCCCCAGTC TCCCCAAGAA CGACGTCATC GTGGCCGTCA ACTGGACGGG TGTGTACTTT 
GTGGATGAGC AGGAGCAGGT ACTTCTGGAG CTGTCCTTCC CAGAGATCAT GGCCGTGTCC 
AGCAGCAGGG GAGCGAAAAC GACGGCCCCC AGCTTCACGC TGGCCACCAT CAAGGGGGAC 
GAATACACCT TCACCTCCAG CAATGCTGAG GACATTCGTG ACCTGGTGGT CACCTTCCTA 
GAGGGGCTCC GGAAGAGATC TAAGTATGTT GTGGCCCTGC AGGATAACCC CAACCCCGCA 
GGCGAGGAGT CAGGCTTCCT CAGCTTTGCC AAGGGAGACC TCATCATCCT GGACCATGAC 
ACGGGCGAGC AGGTCATGAA CTCGGGCTGG GCCAACGGCA TCAATGAGAG GACCAAGCAG 
CGTGGGGACT TCCCCACCGA CAGTGTGTAC GTCATGCCCA CTGTCACCAT GCCACCGCGG 
GAGATTGTGG CCCTGGTCAC CATGACTCCC GATCAGAGGC AGGACGTTGT CCGGCTCTTG 
CAGCTGCGAA CGGCGGAGCC CGAGGTGCGT GCCAAGCCCT ACACGCTGGA GGAGTTTTCC 
TATGACTACT TCAGGCCCCC ACCCAAGCAC ACGCTGAGCC GTGTCATGGT GTCCAAGGCC 
CGAGGCAAGG ACCGGCTGTG GAGCCACACG CGGGAACCGC TCAAGCAGGC GCTGCTCAAG 
AAGCTCCTGG GCAGTGAGGA GCTCTCGCAG GAGGCCTGCC TGGCCTTCAT TGCTGTGCTC 
AAGTACATGG GCGACTACCC GTCCAAGAGG ACACGCTCCG TCAACGAGCT CACCGACCAG 
ATCTTTGAGG GTCCCCTGAA AGCCGAGCCC CTGAAGGACG AGGCATATGT GCAGATCCTG 
AAGCAGCTGA CCGACAACCA CATCAGGTAC AGCGAGGAGC GGGGTTGGGA GCTGCTCTGG 
CTGTGCACGG GCCTTTTCCC ACCCAGCAAC ATCCTCCTGC CCCACGTGCA GCGCTTCCTG 
CAGTCCCGAA AGCACTGCCC ACTCGCCATC GACTGCCTGC AACGGCTCCA GAAAGCCCTG 
AGAAACGGGT CCCGGAAGTA CCCTCCGCAC CTGGTGGAGG TGGAGGCCAT CCAGCACAAG 
ACCACCCAGA TTTTCCACAA AGTCTACTTC CCTGATGACA CTGACGAGGC CTTCGAAGTG 
GAGTCCAGCA CCAAGGCCAA GGACTTCTGC CAGAACATCG CCACCAGGCT GCTCCTCAAG 
TCCTCAGAGG GATTCAGCCT CTTTGTCAAA ATTGCAGACA AGGTCCTCAG CGTTCCTGAG 
AATGACTTCT TCTTTGACTT TGTTCGACAC TTGACAGACT GGATAAAGAA AGCTCGGCCC 
ATCAAGGACG GAATTGTGCC CTCACTCACC TACCAGGTGT TCTTCATGAA GAAGCTGTGG 
ACCACCACGG TGCCAGGGAA GGATCCCATG GCCGATTCCA TCTTCCACTA TTACCAGGAG 
TTGCCCAAGT ATCTCCGAGG CTACCACAAG TGCACGCGGG AGGAGGTGCT GCAGCTGGGG 
GCGCTGATCT ACAGGGTCAA GTTCGAGGAG GACAAGTCCT ACTTCCCCAG CATCCCCAAG 
CTGCTGCGGG AGCTGGTGCC CCAGGACCTT ATCCGGCAGG TCTCACCTGA TGACTGGAAG 
CGGTCCATCG TCGCCTACTT CAACAAGCAC GCAGGGAAGT CCAAGGAGGA GGCCAAGCTG 
GCCTTCCTGA AGCTCATCTT CAAGTGGCCC ACCTTTGGCT CAGCCTTCTT CGAGCAAACT 
ACGGAGCCAA ACTTCCCTGA GATCCTCCTA ATTGCCATCA ACAAGTATGG GGTCAGCCTC 
ATCGATCCCA AAACGAAGGA TATCCTCACC ACTCATCCCT TCACCAAGAT CTCCAACTGG 
AGCAGCGGCA ACACCTACTT CCACATCACC ATTGGGAACT TGGTGCGCGG GAGCAAACTG 
CTCTGCGAGA CGTCACTGGG CTACAAGATG GATGACCTCC TGACTTCCTA CATTAGCCAG 
ATGCTCACAG CCATGAGCAA ACAGCGGGGC TCCAGGAGCG GCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120652.1
CDS	267..6794
Translation	MVILQQGDHVWMDLRLGQEFDVPIGAVVKLCDSGQVQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNSRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQALDERNYHVFYCMLEGMSEDQKKKLGLGQASDYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHLGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVDKINAAIYKPPSQDVKNSRRSIGLLDIFGFENFAVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQDALDMIANKPMNIISLIDEESKFPKGTDTTMLHKLNSQHKLNANYIPPKNNHETQFGINHFAGIVYYETQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRRAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLKLKNAATLIQRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLHQQYRLARQRIIQFQARCRAYLVRKAFRHRLWAVLTVQAYARGMIARRLHQRLRAEYLWRLEAEKMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEAARRKKELLEQMERARHEPVNHSDMVDKMFGFLGTSGGLPGQEGQAPSGFEDLERGRREMVEEDLDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGEAQLPEGQKKSSVRHKLVHLTLKKKSKLTEEVTKRLHDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTAKELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHSPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLNLVPTYIPDREITPLKTLEKWAQLAIAAHKKGIYAQRRTDAQKVKEDVVSYARFKWPLLFSRFYEAYKFSGPSLPKNDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRGAKTTAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDSVYVMPTVTMPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKLLGSEELSQEACLAFIAVLKYMGDYPSKRTRSVNELTDQIFEGPLKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIATRLLLKSSEGFSLFVKIADKVLSVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEQTTEPNFPEILLIAINKYGVSLIDPKTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRGSRSGK

Target ORF information:

RefSeq Version	NM_001127180.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VIIA (MYO7A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127180.1

ORF Insert Sequence:

ATGGTGATTC TTCAGCAGGG GGACCATGTG TGGATGGACC TGAGATTGGG GCAGGAGTTC 
GACGTGCCCA TCGGGGCGGT GGTGAAGCTC TGCGACTCTG GGCAGGTCCA GGTGGTGGAT 
GATGAAGACA ATGAACACTG GATCTCTCCG CAGAACGCAA CGCACATCAA GCCTATGCAC 
CCCACGTCGG TCCACGGCGT GGAGGACATG ATCCGCCTGG GGGACCTCAA CGAGGCGGGC 
ATCTTGCGCA ACCTGCTTAT CCGCTACCGG GACCACCTCA TCTACACGTA TACGGGCTCC 
ATCCTGGTGG CTGTGAACCC CTACCAGCTG CTCTCCATCT ACTCGCCAGA GCACATCCGC 
CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAC 
TGCTACTTCA ACATGAAACG CAACAGCCGA GACCAGTGCT GCATCATCAG TGGGGAATCT 
GGGGCCGGGA AGACGGAGAG CACAAAGCTG ATCCTGCAGT TCCTGGCAGC CATCAGTGGG 
CAGCACTCGT GGATTGAGCA GCAGGTCTTG GAGGCCACCC CCATTCTGGA AGCATTTGGG 
AATGCCAAGA CCATCCGCAA TGACAACTCA AGCCGTTTCG GAAAGTACAT CGACATCCAC 
TTCAACAAGC GGGGCGCCAT CGAGGGCGCG AAGATTGAGC AGTACCTGCT GGAAAAGTCA 
CGTGTCTGTC GCCAGGCCCT GGATGAAAGG AACTACCACG TGTTCTACTG CATGCTGGAG 
GGTATGAGTG AGGATCAGAA GAAGAAGCTG GGCTTGGGCC AGGCCTCTGA CTACAACTAC 
TTGGCCATGG GTAACTGCAT AACCTGTGAG GGCCGGGTGG ACAGCCAGGA GTACGCCAAC 
ATCCGCTCCG CCATGAAGGT GCTCATGTTC ACTGACACCG AGAACTGGGA GATCTCGAAG 
CTCCTGGCTG CCATCCTGCA CCTGGGCAAC CTGCAGTATG AGGCACGCAC ATTTGAAAAC 
CTGGATGCCT GTGAGGTTCT CTTCTCCCCA TCGCTGGCCA CAGCTGCATC CCTGCTTGAG 
GTGAACCCCC CAGACCTGAT GAGCTGCCTG ACTAGCCGCA CCCTCATCAC CCGCGGGGAG 
ACGGTGTCCA CCCCACTGAG CAGGGAACAG GCACTGGACG TGCGCGACGC CTTCGTAAAG 
GGGATCTACG GGCGGCTGTT CGTGTGGATT GTGGACAAGA TCAACGCAGC AATTTACAAG 
CCTCCCTCCC AGGATGTGAA GAACTCTCGC AGGTCCATCG GCCTCCTGGA CATCTTTGGG 
TTTGAGAACT TTGCTGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTCGC CAATGAGCAC 
CTGCAGCAGT TCTTTGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAATA TGACCTGGAG 
AGCATTGACT GGCTGCACAT CGAGTTCACT GACAACCAGG ATGCCCTGGA CATGATTGCC 
AACAAGCCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACA 
GACACCACCA TGTTACACAA GCTGAACTCC CAGCACAAGC TCAACGCCAA CTACATCCCC 
CCCAAGAACA ACCATGAGAC CCAGTTTGGC ATCAACCATT TTGCAGGCAT CGTCTACTAT 
GAGACCCAAG GCTTCCTGGA GAAGAACCGA GACACCCTGC ATGGGGACAT TATCCAGCTG 
GTCCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT CGCCATGGGC 
GCCGAGACCA GGAAGCGCTC GCCCACACTT AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG 
CTGATGCGCA CGCTGGGTGC CTGCCAGCCC TTCTTTGTGC GATGCATCAA GCCCAATGAG 
TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGCGTGC GCCAGCTGCG GTACTCAGGA 
ATGATGGAGA CCATCCGAAT CCGCCGAGCT GGCTACCCCA TCCGCTACAG CTTCGTAGAG 
TTTGTGGAGC GGTACCGTGT GCTGCTGCCA GGTGTGAAGC CGGCCTACAA GCAGGGCGAC 
CTCCGCGGGA CTTGCCAGCG CATGGCTGAG GCTGTGCTGG GCACCCACGA TGACTGGCAG 
ATAGGCAAAA CCAAGATCTT TCTGAAGGAC CACCATGACA TGCTGCTGGA AGTGGAGCGG 
GACAAAGCCA TCACCGACAG AGTCATCCTC CTTCAGAAAG TCATCCGGGG ATTCAAAGAC 
AGGTCTAACT TTCTGAAGCT GAAGAACGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGT 
CACAACTGTA GGAAGAACTA CGGGCTGATG CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG 
CACCGCTCCC GGAAGCTGCA CCAGCAGTAC CGCCTGGCCC GCCAGCGCAT CATCCAGTTC 
CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC AAGGCCTTCC GCCACCGCCT CTGGGCTGTG 
CTCACCGTGC AGGCCTATGC CCGGGGCATG ATCGCCCGCA GGCTGCACCA ACGCCTCAGG 
GCTGAGTATC TGTGGCGCCT CGAGGCTGAG AAAATGCGGC TGGCGGAGGA AGAGAAGCTT 
CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC 
CTGGCCCAGC TGGCTCGTGA GGACGCTGAG CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG 
CGGAAGAAGG AGCTCCTGGA GCAGATGGAA AGGGCCCGCC ATGAGCCTGT CAATCACTCA 
GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCAG GTGGCCTGCC AGGCCAGGAG 
GGCCAGGCAC CTAGTGGCTT TGAGGACCTG GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG 
GACCTGGATG CAGCCCTGCC CCTGCCTGAC GAGGATGAGG AGGACCTCTC TGAGTATAAA 
TTTGCCAAGT TCGCGGCCAC CTACTTCCAG GGGACAACCA CGCACTCCTA CACCCGGCGG 
CCACTCAAAC AGCCACTGCT CTACCATGAC GACGAGGGTG ACCAGCTGGC AGCCCTGGCG 
GTCTGGATCA CCATCCTCCG CTTCATGGGG GACCTCCCTG AGCCCAAGTA CCACACAGCC 
ATGAGTGATG GCAGTGAGAA GATCCCTGTG ATGACCAAGA TTTATGAGAC CCTGGGCAAG 
AAGACGTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG 
GGCCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCATT TGACTCTGAA AAAGAAGTCC 
AAGCTCACAG AGGAGGTGAC CAAGAGGCTG CATGACGGGG AGTCCACAGT GCAGGGCAAC 
AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATCGGCAAT 
GGCATCCTGC GGCCAGCACT CCGGGACGAG ATCTACTGCC AGATCAGCAA GCAGCTGACC 
CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATTC TCGTGTCTCT CTGCGTGGGC 
TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG TACCTGCGGA ACTTCATCCA CGGGGGCCCG 
CCCGGCTACG CCCCGTACTG TGAGGAGCGC CTGAGAAGGA CCTTTGTCAA TGGGACACGG 
ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC AATCATGTTG 
CCCGTGACAT TCATGGATGG GACCACCAAG ACCCTGCTGA CGGACTCGGC AACCACGGCC 
AAGGAGCTCT GCAACGCGCT GGCCGACAAG ATCTCTCTCA AGGACCGGTT CGGGTTCTCC 
CTCTACATTG CCCTGTTTGA CAAGGTGTCC TCCCTGGGCA GCGGCAGTGA CCACGTCATG 
GACGCCATCT CCCAGTGCGA GCAGTACGCC AAGGAGCAGG GCGCCCAGGA GCGCAACGCC 
CCCTGGAGGC TCTTCTTCCG CAAAGAGGTC TTCACGCCCT GGCACAGCCC CTCCGAGGAC 
AACGTGGCCA CCAACCTCAT CTACCAGCAG GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC 
AGGTGTGAGA AGGAGGACGA CCTGGCTGAG CTGGCCTCCC AGCAGTACTT TGTAGACTAT 
GGCTCTGAGA TGATCCTGGA GCGCCTCCTG AACCTCGTGC CCACCTACAT CCCCGACCGC 
GAGATCACGC CCCTGAAGAC GCTGGAGAAG TGGGCCCAGC TGGCCATCGC CGCCCACAAG 
AAGGGGATTT ATGCCCAGAG GAGAACTGAT GCCCAGAAGG TCAAAGAGGA TGTGGTCAGT 
TATGCCCGCT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ATGAAGCCTA CAAATTCTCA 
GGCCCCAGTC TCCCCAAGAA CGACGTCATC GTGGCCGTCA ACTGGACGGG TGTGTACTTT 
GTGGATGAGC AGGAGCAGGT ACTTCTGGAG CTGTCCTTCC CAGAGATCAT GGCCGTGTCC 
AGCAGCAGGG GAGCGAAAAC GACGGCCCCC AGCTTCACGC TGGCCACCAT CAAGGGGGAC 
GAATACACCT TCACCTCCAG CAATGCTGAG GACATTCGTG ACCTGGTGGT CACCTTCCTA 
GAGGGGCTCC GGAAGAGATC TAAGTATGTT GTGGCCCTGC AGGATAACCC CAACCCCGCA 
GGCGAGGAGT CAGGCTTCCT CAGCTTTGCC AAGGGAGACC TCATCATCCT GGACCATGAC 
ACGGGCGAGC AGGTCATGAA CTCGGGCTGG GCCAACGGCA TCAATGAGAG GACCAAGCAG 
CGTGGGGACT TCCCCACCGA CAGTGTGTAC GTCATGCCCA CTGTCACCAT GCCACCGCGG 
GAGATTGTGG CCCTGGTCAC CATGACTCCC GATCAGAGGC AGGACGTTGT CCGGCTCTTG 
CAGCTGCGAA CGGCGGAGCC CGAGGTGCGT GCCAAGCCCT ACACGCTGGA GGAGTTTTCC 
TATGACTACT TCAGGCCCCC ACCCAAGCAC ACGCTGAGCC GTGTCATGGT GTCCAAGGCC 
CGAGGCAAGG ACCGGCTGTG GAGCCACACG CGGGAACCGC TCAAGCAGGC GCTGCTCAAG 
AAGCTCCTGG GCAGTGAGGA GCTCTCGCAG GAGGCCTGCC TGGCCTTCAT TGCTGTGCTC 
AAGTACATGG GCGACTACCC GTCCAAGAGG ACACGCTCCG TCAACGAGCT CACCGACCAG 
ATCTTTGAGG GTCCCCTGAA AGCCGAGCCC CTGAAGGACG AGGCATATGT GCAGATCCTG 
AAGCAGCTGA CCGACAACCA CATCAGGTAC AGCGAGGAGC GGGGTTGGGA GCTGCTCTGG 
CTGTGCACGG GCCTTTTCCC ACCCAGCAAC ATCCTCCTGC CCCACGTGCA GCGCTTCCTG 
CAGTCCCGAA AGCACTGCCC ACTCGCCATC GACTGCCTGC AACGGCTCCA GAAAGCCCTG 
AGAAACGGGT CCCGGAAGTA CCCTCCGCAC CTGGTGGAGG TGGAGGCCAT CCAGCACAAG 
ACCACCCAGA TTTTCCACAA AGTCTACTTC CCTGATGACA CTGACGAGGC CTTCGAAGTG 
GAGTCCAGCA CCAAGGCCAA GGACTTCTGC CAGAACATCG CCACCAGGCT GCTCCTCAAG 
TCCTCAGAGG GATTCAGCCT CTTTGTCAAA ATTGCAGACA AGGTCCTCAG CGTTCCTGAG 
AATGACTTCT TCTTTGACTT TGTTCGACAC TTGACAGACT GGATAAAGAA AGCTCGGCCC 
ATCAAGGACG GAATTGTGCC CTCACTCACC TACCAGGTGT TCTTCATGAA GAAGCTGTGG 
ACCACCACGG TGCCAGGGAA GGATCCCATG GCCGATTCCA TCTTCCACTA TTACCAGGAG 
TTGCCCAAGT ATCTCCGAGG CTACCACAAG TGCACGCGGG AGGAGGTGCT GCAGCTGGGG 
GCGCTGATCT ACAGGGTCAA GTTCGAGGAG GACAAGTCCT ACTTCCCCAG CATCCCCAAG 
CTGCTGCGGG AGCTGGTGCC CCAGGACCTT ATCCGGCAGG TCTCACCTGA TGACTGGAAG 
CGGTCCATCG TCGCCTACTT CAACAAGCAC GCAGGGAAGT CCAAGGAGGA GGCCAAGCTG 
GCCTTCCTGA AGCTCATCTT CAAGTGGCCC ACCTTTGGCT CAGCCTTCTT CGAGCAAACT 
ACGGAGCCAA ACTTCCCTGA GATCCTCCTA ATTGCCATCA ACAAGTATGG GGTCAGCCTC 
ATCGATCCCA AAACGAAGGA TATCCTCACC ACTCATCCCT TCACCAAGAT CTCCAACTGG 
AGCAGCGGCA ACACCTACTT CCACATCACC ATTGGGAACT TGGTGCGCGG GAGCAAACTG 
CTCTGCGAGA CGTCACTGGG CTACAAGATG GATGACCTCC TGACTTCCTA CATTAGCCAG 
ATGCTCACAG CCATGAGCAA ACAGCGGGGC TCCAGGAGCG GCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO7A ( NM_001127180.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001120652.1 Homo sapiens myosin VIIA (MYO7A), transcript variant 2, mRNA.

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CloneID	OHu26178
Clone ID Related Accession (Same CDS sequence)	NM_001127180.1 , NM_001127180.2
Accession Version	NM_001127180.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Homo sapiens(human)
Product	unconventional myosin-VIIa isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000752.4, U55208.1, U39226.1 and BG108466.1. On Jun 1, 2019 this sequence version replaced NM_001127180.1. Transcript Variant: This variant (2) lacks an in-frame segment in the coding region, compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U55208.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGATTC TTCAGCAGGG GGACCATGTG TGGATGGACC TGAGATTGGG GCAGGAGTTC 
GACGTGCCCA TCGGGGCGGT GGTGAAGCTC TGCGACTCTG GGCAGGTCCA GGTGGTGGAT 
GATGAAGACA ATGAACACTG GATCTCTCCG CAGAACGCAA CGCACATCAA GCCTATGCAC 
CCCACGTCGG TCCACGGCGT GGAGGACATG ATCCGCCTGG GGGACCTCAA CGAGGCGGGC 
ATCTTGCGCA ACCTGCTTAT CCGCTACCGG GACCACCTCA TCTACACGTA TACGGGCTCC 
ATCCTGGTGG CTGTGAACCC CTACCAGCTG CTCTCCATCT ACTCGCCAGA GCACATCCGC 
CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAC 
TGCTACTTCA ACATGAAACG CAACAGCCGA GACCAGTGCT GCATCATCAG TGGGGAATCT 
GGGGCCGGGA AGACGGAGAG CACAAAGCTG ATCCTGCAGT TCCTGGCAGC CATCAGTGGG 
CAGCACTCGT GGATTGAGCA GCAGGTCTTG GAGGCCACCC CCATTCTGGA AGCATTTGGG 
AATGCCAAGA CCATCCGCAA TGACAACTCA AGCCGTTTCG GAAAGTACAT CGACATCCAC 
TTCAACAAGC GGGGCGCCAT CGAGGGCGCG AAGATTGAGC AGTACCTGCT GGAAAAGTCA 
CGTGTCTGTC GCCAGGCCCT GGATGAAAGG AACTACCACG TGTTCTACTG CATGCTGGAG 
GGTATGAGTG AGGATCAGAA GAAGAAGCTG GGCTTGGGCC AGGCCTCTGA CTACAACTAC 
TTGGCCATGG GTAACTGCAT AACCTGTGAG GGCCGGGTGG ACAGCCAGGA GTACGCCAAC 
ATCCGCTCCG CCATGAAGGT GCTCATGTTC ACTGACACCG AGAACTGGGA GATCTCGAAG 
CTCCTGGCTG CCATCCTGCA CCTGGGCAAC CTGCAGTATG AGGCACGCAC ATTTGAAAAC 
CTGGATGCCT GTGAGGTTCT CTTCTCCCCA TCGCTGGCCA CAGCTGCATC CCTGCTTGAG 
GTGAACCCCC CAGACCTGAT GAGCTGCCTG ACTAGCCGCA CCCTCATCAC CCGCGGGGAG 
ACGGTGTCCA CCCCACTGAG CAGGGAACAG GCACTGGACG TGCGCGACGC CTTCGTAAAG 
GGGATCTACG GGCGGCTGTT CGTGTGGATT GTGGACAAGA TCAACGCAGC AATTTACAAG 
CCTCCCTCCC AGGATGTGAA GAACTCTCGC AGGTCCATCG GCCTCCTGGA CATCTTTGGG 
TTTGAGAACT TTGCTGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTCGC CAATGAGCAC 
CTGCAGCAGT TCTTTGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAATA TGACCTGGAG 
AGCATTGACT GGCTGCACAT CGAGTTCACT GACAACCAGG ATGCCCTGGA CATGATTGCC 
AACAAGCCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACA 
GACACCACCA TGTTACACAA GCTGAACTCC CAGCACAAGC TCAACGCCAA CTACATCCCC 
CCCAAGAACA ACCATGAGAC CCAGTTTGGC ATCAACCATT TTGCAGGCAT CGTCTACTAT 
GAGACCCAAG GCTTCCTGGA GAAGAACCGA GACACCCTGC ATGGGGACAT TATCCAGCTG 
GTCCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT CGCCATGGGC 
GCCGAGACCA GGAAGCGCTC GCCCACACTT AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG 
CTGATGCGCA CGCTGGGTGC CTGCCAGCCC TTCTTTGTGC GATGCATCAA GCCCAATGAG 
TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGCGTGC GCCAGCTGCG GTACTCAGGA 
ATGATGGAGA CCATCCGAAT CCGCCGAGCT GGCTACCCCA TCCGCTACAG CTTCGTAGAG 
TTTGTGGAGC GGTACCGTGT GCTGCTGCCA GGTGTGAAGC CGGCCTACAA GCAGGGCGAC 
CTCCGCGGGA CTTGCCAGCG CATGGCTGAG GCTGTGCTGG GCACCCACGA TGACTGGCAG 
ATAGGCAAAA CCAAGATCTT TCTGAAGGAC CACCATGACA TGCTGCTGGA AGTGGAGCGG 
GACAAAGCCA TCACCGACAG AGTCATCCTC CTTCAGAAAG TCATCCGGGG ATTCAAAGAC 
AGGTCTAACT TTCTGAAGCT GAAGAACGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGT 
CACAACTGTA GGAAGAACTA CGGGCTGATG CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG 
CACCGCTCCC GGAAGCTGCA CCAGCAGTAC CGCCTGGCCC GCCAGCGCAT CATCCAGTTC 
CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC AAGGCCTTCC GCCACCGCCT CTGGGCTGTG 
CTCACCGTGC AGGCCTATGC CCGGGGCATG ATCGCCCGCA GGCTGCACCA ACGCCTCAGG 
GCTGAGTATC TGTGGCGCCT CGAGGCTGAG AAAATGCGGC TGGCGGAGGA AGAGAAGCTT 
CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC 
CTGGCCCAGC TGGCTCGTGA GGACGCTGAG CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG 
CGGAAGAAGG AGCTCCTGGA GCAGATGGAA AGGGCCCGCC ATGAGCCTGT CAATCACTCA 
GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCAG GTGGCCTGCC AGGCCAGGAG 
GGCCAGGCAC CTAGTGGCTT TGAGGACCTG GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG 
GACCTGGATG CAGCCCTGCC CCTGCCTGAC GAGGATGAGG AGGACCTCTC TGAGTATAAA 
TTTGCCAAGT TCGCGGCCAC CTACTTCCAG GGGACAACCA CGCACTCCTA CACCCGGCGG 
CCACTCAAAC AGCCACTGCT CTACCATGAC GACGAGGGTG ACCAGCTGGC AGCCCTGGCG 
GTCTGGATCA CCATCCTCCG CTTCATGGGG GACCTCCCTG AGCCCAAGTA CCACACAGCC 
ATGAGTGATG GCAGTGAGAA GATCCCTGTG ATGACCAAGA TTTATGAGAC CCTGGGCAAG 
AAGACGTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG 
GGCCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCATT TGACTCTGAA AAAGAAGTCC 
AAGCTCACAG AGGAGGTGAC CAAGAGGCTG CATGACGGGG AGTCCACAGT GCAGGGCAAC 
AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATCGGCAAT 
GGCATCCTGC GGCCAGCACT CCGGGACGAG ATCTACTGCC AGATCAGCAA GCAGCTGACC 
CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATTC TCGTGTCTCT CTGCGTGGGC 
TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG TACCTGCGGA ACTTCATCCA CGGGGGCCCG 
CCCGGCTACG CCCCGTACTG TGAGGAGCGC CTGAGAAGGA CCTTTGTCAA TGGGACACGG 
ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC AATCATGTTG 
CCCGTGACAT TCATGGATGG GACCACCAAG ACCCTGCTGA CGGACTCGGC AACCACGGCC 
AAGGAGCTCT GCAACGCGCT GGCCGACAAG ATCTCTCTCA AGGACCGGTT CGGGTTCTCC 
CTCTACATTG CCCTGTTTGA CAAGGTGTCC TCCCTGGGCA GCGGCAGTGA CCACGTCATG 
GACGCCATCT CCCAGTGCGA GCAGTACGCC AAGGAGCAGG GCGCCCAGGA GCGCAACGCC 
CCCTGGAGGC TCTTCTTCCG CAAAGAGGTC TTCACGCCCT GGCACAGCCC CTCCGAGGAC 
AACGTGGCCA CCAACCTCAT CTACCAGCAG GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC 
AGGTGTGAGA AGGAGGACGA CCTGGCTGAG CTGGCCTCCC AGCAGTACTT TGTAGACTAT 
GGCTCTGAGA TGATCCTGGA GCGCCTCCTG AACCTCGTGC CCACCTACAT CCCCGACCGC 
GAGATCACGC CCCTGAAGAC GCTGGAGAAG TGGGCCCAGC TGGCCATCGC CGCCCACAAG 
AAGGGGATTT ATGCCCAGAG GAGAACTGAT GCCCAGAAGG TCAAAGAGGA TGTGGTCAGT 
TATGCCCGCT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ATGAAGCCTA CAAATTCTCA 
GGCCCCAGTC TCCCCAAGAA CGACGTCATC GTGGCCGTCA ACTGGACGGG TGTGTACTTT 
GTGGATGAGC AGGAGCAGGT ACTTCTGGAG CTGTCCTTCC CAGAGATCAT GGCCGTGTCC 
AGCAGCAGGG GAGCGAAAAC GACGGCCCCC AGCTTCACGC TGGCCACCAT CAAGGGGGAC 
GAATACACCT TCACCTCCAG CAATGCTGAG GACATTCGTG ACCTGGTGGT CACCTTCCTA 
GAGGGGCTCC GGAAGAGATC TAAGTATGTT GTGGCCCTGC AGGATAACCC CAACCCCGCA 
GGCGAGGAGT CAGGCTTCCT CAGCTTTGCC AAGGGAGACC TCATCATCCT GGACCATGAC 
ACGGGCGAGC AGGTCATGAA CTCGGGCTGG GCCAACGGCA TCAATGAGAG GACCAAGCAG 
CGTGGGGACT TCCCCACCGA CAGTGTGTAC GTCATGCCCA CTGTCACCAT GCCACCGCGG 
GAGATTGTGG CCCTGGTCAC CATGACTCCC GATCAGAGGC AGGACGTTGT CCGGCTCTTG 
CAGCTGCGAA CGGCGGAGCC CGAGGTGCGT GCCAAGCCCT ACACGCTGGA GGAGTTTTCC 
TATGACTACT TCAGGCCCCC ACCCAAGCAC ACGCTGAGCC GTGTCATGGT GTCCAAGGCC 
CGAGGCAAGG ACCGGCTGTG GAGCCACACG CGGGAACCGC TCAAGCAGGC GCTGCTCAAG 
AAGCTCCTGG GCAGTGAGGA GCTCTCGCAG GAGGCCTGCC TGGCCTTCAT TGCTGTGCTC 
AAGTACATGG GCGACTACCC GTCCAAGAGG ACACGCTCCG TCAACGAGCT CACCGACCAG 
ATCTTTGAGG GTCCCCTGAA AGCCGAGCCC CTGAAGGACG AGGCATATGT GCAGATCCTG 
AAGCAGCTGA CCGACAACCA CATCAGGTAC AGCGAGGAGC GGGGTTGGGA GCTGCTCTGG 
CTGTGCACGG GCCTTTTCCC ACCCAGCAAC ATCCTCCTGC CCCACGTGCA GCGCTTCCTG 
CAGTCCCGAA AGCACTGCCC ACTCGCCATC GACTGCCTGC AACGGCTCCA GAAAGCCCTG 
AGAAACGGGT CCCGGAAGTA CCCTCCGCAC CTGGTGGAGG TGGAGGCCAT CCAGCACAAG 
ACCACCCAGA TTTTCCACAA AGTCTACTTC CCTGATGACA CTGACGAGGC CTTCGAAGTG 
GAGTCCAGCA CCAAGGCCAA GGACTTCTGC CAGAACATCG CCACCAGGCT GCTCCTCAAG 
TCCTCAGAGG GATTCAGCCT CTTTGTCAAA ATTGCAGACA AGGTCCTCAG CGTTCCTGAG 
AATGACTTCT TCTTTGACTT TGTTCGACAC TTGACAGACT GGATAAAGAA AGCTCGGCCC 
ATCAAGGACG GAATTGTGCC CTCACTCACC TACCAGGTGT TCTTCATGAA GAAGCTGTGG 
ACCACCACGG TGCCAGGGAA GGATCCCATG GCCGATTCCA TCTTCCACTA TTACCAGGAG 
TTGCCCAAGT ATCTCCGAGG CTACCACAAG TGCACGCGGG AGGAGGTGCT GCAGCTGGGG 
GCGCTGATCT ACAGGGTCAA GTTCGAGGAG GACAAGTCCT ACTTCCCCAG CATCCCCAAG 
CTGCTGCGGG AGCTGGTGCC CCAGGACCTT ATCCGGCAGG TCTCACCTGA TGACTGGAAG 
CGGTCCATCG TCGCCTACTT CAACAAGCAC GCAGGGAAGT CCAAGGAGGA GGCCAAGCTG 
GCCTTCCTGA AGCTCATCTT CAAGTGGCCC ACCTTTGGCT CAGCCTTCTT CGAGCAAACT 
ACGGAGCCAA ACTTCCCTGA GATCCTCCTA ATTGCCATCA ACAAGTATGG GGTCAGCCTC 
ATCGATCCCA AAACGAAGGA TATCCTCACC ACTCATCCCT TCACCAAGAT CTCCAACTGG 
AGCAGCGGCA ACACCTACTT CCACATCACC ATTGGGAACT TGGTGCGCGG GAGCAAACTG 
CTCTGCGAGA CGTCACTGGG CTACAAGATG GATGACCTCC TGACTTCCTA CATTAGCCAG 
ATGCTCACAG CCATGAGCAA ACAGCGGGGC TCCAGGAGCG GCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120652.1
CDS	291..6818
Translation	MVILQQGDHVWMDLRLGQEFDVPIGAVVKLCDSGQVQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNSRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQALDERNYHVFYCMLEGMSEDQKKKLGLGQASDYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHLGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVDKINAAIYKPPSQDVKNSRRSIGLLDIFGFENFAVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQDALDMIANKPMNIISLIDEESKFPKGTDTTMLHKLNSQHKLNANYIPPKNNHETQFGINHFAGIVYYETQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRRAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLKLKNAATLIQRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLHQQYRLARQRIIQFQARCRAYLVRKAFRHRLWAVLTVQAYARGMIARRLHQRLRAEYLWRLEAEKMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEAARRKKELLEQMERARHEPVNHSDMVDKMFGFLGTSGGLPGQEGQAPSGFEDLERGRREMVEEDLDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGEAQLPEGQKKSSVRHKLVHLTLKKKSKLTEEVTKRLHDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTAKELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHSPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLNLVPTYIPDREITPLKTLEKWAQLAIAAHKKGIYAQRRTDAQKVKEDVVSYARFKWPLLFSRFYEAYKFSGPSLPKNDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRGAKTTAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDSVYVMPTVTMPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKLLGSEELSQEACLAFIAVLKYMGDYPSKRTRSVNELTDQIFEGPLKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIATRLLLKSSEGFSLFVKIADKVLSVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEQTTEPNFPEILLIAINKYGVSLIDPKTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRGSRSGK

Target ORF information:

RefSeq Version	NM_001127180.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VIIA (MYO7A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127180.2

ORF Insert Sequence:

ATGGTGATTC TTCAGCAGGG GGACCATGTG TGGATGGACC TGAGATTGGG GCAGGAGTTC 
GACGTGCCCA TCGGGGCGGT GGTGAAGCTC TGCGACTCTG GGCAGGTCCA GGTGGTGGAT 
GATGAAGACA ATGAACACTG GATCTCTCCG CAGAACGCAA CGCACATCAA GCCTATGCAC 
CCCACGTCGG TCCACGGCGT GGAGGACATG ATCCGCCTGG GGGACCTCAA CGAGGCGGGC 
ATCTTGCGCA ACCTGCTTAT CCGCTACCGG GACCACCTCA TCTACACGTA TACGGGCTCC 
ATCCTGGTGG CTGTGAACCC CTACCAGCTG CTCTCCATCT ACTCGCCAGA GCACATCCGC 
CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAC 
TGCTACTTCA ACATGAAACG CAACAGCCGA GACCAGTGCT GCATCATCAG TGGGGAATCT 
GGGGCCGGGA AGACGGAGAG CACAAAGCTG ATCCTGCAGT TCCTGGCAGC CATCAGTGGG 
CAGCACTCGT GGATTGAGCA GCAGGTCTTG GAGGCCACCC CCATTCTGGA AGCATTTGGG 
AATGCCAAGA CCATCCGCAA TGACAACTCA AGCCGTTTCG GAAAGTACAT CGACATCCAC 
TTCAACAAGC GGGGCGCCAT CGAGGGCGCG AAGATTGAGC AGTACCTGCT GGAAAAGTCA 
CGTGTCTGTC GCCAGGCCCT GGATGAAAGG AACTACCACG TGTTCTACTG CATGCTGGAG 
GGTATGAGTG AGGATCAGAA GAAGAAGCTG GGCTTGGGCC AGGCCTCTGA CTACAACTAC 
TTGGCCATGG GTAACTGCAT AACCTGTGAG GGCCGGGTGG ACAGCCAGGA GTACGCCAAC 
ATCCGCTCCG CCATGAAGGT GCTCATGTTC ACTGACACCG AGAACTGGGA GATCTCGAAG 
CTCCTGGCTG CCATCCTGCA CCTGGGCAAC CTGCAGTATG AGGCACGCAC ATTTGAAAAC 
CTGGATGCCT GTGAGGTTCT CTTCTCCCCA TCGCTGGCCA CAGCTGCATC CCTGCTTGAG 
GTGAACCCCC CAGACCTGAT GAGCTGCCTG ACTAGCCGCA CCCTCATCAC CCGCGGGGAG 
ACGGTGTCCA CCCCACTGAG CAGGGAACAG GCACTGGACG TGCGCGACGC CTTCGTAAAG 
GGGATCTACG GGCGGCTGTT CGTGTGGATT GTGGACAAGA TCAACGCAGC AATTTACAAG 
CCTCCCTCCC AGGATGTGAA GAACTCTCGC AGGTCCATCG GCCTCCTGGA CATCTTTGGG 
TTTGAGAACT TTGCTGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTCGC CAATGAGCAC 
CTGCAGCAGT TCTTTGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAATA TGACCTGGAG 
AGCATTGACT GGCTGCACAT CGAGTTCACT GACAACCAGG ATGCCCTGGA CATGATTGCC 
AACAAGCCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACA 
GACACCACCA TGTTACACAA GCTGAACTCC CAGCACAAGC TCAACGCCAA CTACATCCCC 
CCCAAGAACA ACCATGAGAC CCAGTTTGGC ATCAACCATT TTGCAGGCAT CGTCTACTAT 
GAGACCCAAG GCTTCCTGGA GAAGAACCGA GACACCCTGC ATGGGGACAT TATCCAGCTG 
GTCCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT CGCCATGGGC 
GCCGAGACCA GGAAGCGCTC GCCCACACTT AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG 
CTGATGCGCA CGCTGGGTGC CTGCCAGCCC TTCTTTGTGC GATGCATCAA GCCCAATGAG 
TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGCGTGC GCCAGCTGCG GTACTCAGGA 
ATGATGGAGA CCATCCGAAT CCGCCGAGCT GGCTACCCCA TCCGCTACAG CTTCGTAGAG 
TTTGTGGAGC GGTACCGTGT GCTGCTGCCA GGTGTGAAGC CGGCCTACAA GCAGGGCGAC 
CTCCGCGGGA CTTGCCAGCG CATGGCTGAG GCTGTGCTGG GCACCCACGA TGACTGGCAG 
ATAGGCAAAA CCAAGATCTT TCTGAAGGAC CACCATGACA TGCTGCTGGA AGTGGAGCGG 
GACAAAGCCA TCACCGACAG AGTCATCCTC CTTCAGAAAG TCATCCGGGG ATTCAAAGAC 
AGGTCTAACT TTCTGAAGCT GAAGAACGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGT 
CACAACTGTA GGAAGAACTA CGGGCTGATG CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG 
CACCGCTCCC GGAAGCTGCA CCAGCAGTAC CGCCTGGCCC GCCAGCGCAT CATCCAGTTC 
CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC AAGGCCTTCC GCCACCGCCT CTGGGCTGTG 
CTCACCGTGC AGGCCTATGC CCGGGGCATG ATCGCCCGCA GGCTGCACCA ACGCCTCAGG 
GCTGAGTATC TGTGGCGCCT CGAGGCTGAG AAAATGCGGC TGGCGGAGGA AGAGAAGCTT 
CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC 
CTGGCCCAGC TGGCTCGTGA GGACGCTGAG CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG 
CGGAAGAAGG AGCTCCTGGA GCAGATGGAA AGGGCCCGCC ATGAGCCTGT CAATCACTCA 
GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCAG GTGGCCTGCC AGGCCAGGAG 
GGCCAGGCAC CTAGTGGCTT TGAGGACCTG GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG 
GACCTGGATG CAGCCCTGCC CCTGCCTGAC GAGGATGAGG AGGACCTCTC TGAGTATAAA 
TTTGCCAAGT TCGCGGCCAC CTACTTCCAG GGGACAACCA CGCACTCCTA CACCCGGCGG 
CCACTCAAAC AGCCACTGCT CTACCATGAC GACGAGGGTG ACCAGCTGGC AGCCCTGGCG 
GTCTGGATCA CCATCCTCCG CTTCATGGGG GACCTCCCTG AGCCCAAGTA CCACACAGCC 
ATGAGTGATG GCAGTGAGAA GATCCCTGTG ATGACCAAGA TTTATGAGAC CCTGGGCAAG 
AAGACGTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG 
GGCCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCATT TGACTCTGAA AAAGAAGTCC 
AAGCTCACAG AGGAGGTGAC CAAGAGGCTG CATGACGGGG AGTCCACAGT GCAGGGCAAC 
AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATCGGCAAT 
GGCATCCTGC GGCCAGCACT CCGGGACGAG ATCTACTGCC AGATCAGCAA GCAGCTGACC 
CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATTC TCGTGTCTCT CTGCGTGGGC 
TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG TACCTGCGGA ACTTCATCCA CGGGGGCCCG 
CCCGGCTACG CCCCGTACTG TGAGGAGCGC CTGAGAAGGA CCTTTGTCAA TGGGACACGG 
ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC AATCATGTTG 
CCCGTGACAT TCATGGATGG GACCACCAAG ACCCTGCTGA CGGACTCGGC AACCACGGCC 
AAGGAGCTCT GCAACGCGCT GGCCGACAAG ATCTCTCTCA AGGACCGGTT CGGGTTCTCC 
CTCTACATTG CCCTGTTTGA CAAGGTGTCC TCCCTGGGCA GCGGCAGTGA CCACGTCATG 
GACGCCATCT CCCAGTGCGA GCAGTACGCC AAGGAGCAGG GCGCCCAGGA GCGCAACGCC 
CCCTGGAGGC TCTTCTTCCG CAAAGAGGTC TTCACGCCCT GGCACAGCCC CTCCGAGGAC 
AACGTGGCCA CCAACCTCAT CTACCAGCAG GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC 
AGGTGTGAGA AGGAGGACGA CCTGGCTGAG CTGGCCTCCC AGCAGTACTT TGTAGACTAT 
GGCTCTGAGA TGATCCTGGA GCGCCTCCTG AACCTCGTGC CCACCTACAT CCCCGACCGC 
GAGATCACGC CCCTGAAGAC GCTGGAGAAG TGGGCCCAGC TGGCCATCGC CGCCCACAAG 
AAGGGGATTT ATGCCCAGAG GAGAACTGAT GCCCAGAAGG TCAAAGAGGA TGTGGTCAGT 
TATGCCCGCT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ATGAAGCCTA CAAATTCTCA 
GGCCCCAGTC TCCCCAAGAA CGACGTCATC GTGGCCGTCA ACTGGACGGG TGTGTACTTT 
GTGGATGAGC AGGAGCAGGT ACTTCTGGAG CTGTCCTTCC CAGAGATCAT GGCCGTGTCC 
AGCAGCAGGG GAGCGAAAAC GACGGCCCCC AGCTTCACGC TGGCCACCAT CAAGGGGGAC 
GAATACACCT TCACCTCCAG CAATGCTGAG GACATTCGTG ACCTGGTGGT CACCTTCCTA 
GAGGGGCTCC GGAAGAGATC TAAGTATGTT GTGGCCCTGC AGGATAACCC CAACCCCGCA 
GGCGAGGAGT CAGGCTTCCT CAGCTTTGCC AAGGGAGACC TCATCATCCT GGACCATGAC 
ACGGGCGAGC AGGTCATGAA CTCGGGCTGG GCCAACGGCA TCAATGAGAG GACCAAGCAG 
CGTGGGGACT TCCCCACCGA CAGTGTGTAC GTCATGCCCA CTGTCACCAT GCCACCGCGG 
GAGATTGTGG CCCTGGTCAC CATGACTCCC GATCAGAGGC AGGACGTTGT CCGGCTCTTG 
CAGCTGCGAA CGGCGGAGCC CGAGGTGCGT GCCAAGCCCT ACACGCTGGA GGAGTTTTCC 
TATGACTACT TCAGGCCCCC ACCCAAGCAC ACGCTGAGCC GTGTCATGGT GTCCAAGGCC 
CGAGGCAAGG ACCGGCTGTG GAGCCACACG CGGGAACCGC TCAAGCAGGC GCTGCTCAAG 
AAGCTCCTGG GCAGTGAGGA GCTCTCGCAG GAGGCCTGCC TGGCCTTCAT TGCTGTGCTC 
AAGTACATGG GCGACTACCC GTCCAAGAGG ACACGCTCCG TCAACGAGCT CACCGACCAG 
ATCTTTGAGG GTCCCCTGAA AGCCGAGCCC CTGAAGGACG AGGCATATGT GCAGATCCTG 
AAGCAGCTGA CCGACAACCA CATCAGGTAC AGCGAGGAGC GGGGTTGGGA GCTGCTCTGG 
CTGTGCACGG GCCTTTTCCC ACCCAGCAAC ATCCTCCTGC CCCACGTGCA GCGCTTCCTG 
CAGTCCCGAA AGCACTGCCC ACTCGCCATC GACTGCCTGC AACGGCTCCA GAAAGCCCTG 
AGAAACGGGT CCCGGAAGTA CCCTCCGCAC CTGGTGGAGG TGGAGGCCAT CCAGCACAAG 
ACCACCCAGA TTTTCCACAA AGTCTACTTC CCTGATGACA CTGACGAGGC CTTCGAAGTG 
GAGTCCAGCA CCAAGGCCAA GGACTTCTGC CAGAACATCG CCACCAGGCT GCTCCTCAAG 
TCCTCAGAGG GATTCAGCCT CTTTGTCAAA ATTGCAGACA AGGTCCTCAG CGTTCCTGAG 
AATGACTTCT TCTTTGACTT TGTTCGACAC TTGACAGACT GGATAAAGAA AGCTCGGCCC 
ATCAAGGACG GAATTGTGCC CTCACTCACC TACCAGGTGT TCTTCATGAA GAAGCTGTGG 
ACCACCACGG TGCCAGGGAA GGATCCCATG GCCGATTCCA TCTTCCACTA TTACCAGGAG 
TTGCCCAAGT ATCTCCGAGG CTACCACAAG TGCACGCGGG AGGAGGTGCT GCAGCTGGGG 
GCGCTGATCT ACAGGGTCAA GTTCGAGGAG GACAAGTCCT ACTTCCCCAG CATCCCCAAG 
CTGCTGCGGG AGCTGGTGCC CCAGGACCTT ATCCGGCAGG TCTCACCTGA TGACTGGAAG 
CGGTCCATCG TCGCCTACTT CAACAAGCAC GCAGGGAAGT CCAAGGAGGA GGCCAAGCTG 
GCCTTCCTGA AGCTCATCTT CAAGTGGCCC ACCTTTGGCT CAGCCTTCTT CGAGCAAACT 
ACGGAGCCAA ACTTCCCTGA GATCCTCCTA ATTGCCATCA ACAAGTATGG GGTCAGCCTC 
ATCGATCCCA AAACGAAGGA TATCCTCACC ACTCATCCCT TCACCAAGAT CTCCAACTGG 
AGCAGCGGCA ACACCTACTT CCACATCACC ATTGGGAACT TGGTGCGCGG GAGCAAACTG 
CTCTGCGAGA CGTCACTGGG CTACAAGATG GATGACCTCC TGACTTCCTA CATTAGCCAG 
ATGCTCACAG CCATGAGCAA ACAGCGGGGC TCCAGGAGCG GCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO7A ( NM_000260.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000251.3 Homo sapiens myosin VIIA (MYO7A), transcript variant 1, mRNA.

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CloneID	OHu27355
Clone ID Related Accession (Same CDS sequence)	NM_000260.3 , NM_000260.4
Accession Version	NM_000260.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	unconventional myosin-VIIa isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U39226.1, U34227.1, U55208.1 and BG108466.1. This sequence is a reference standard in the RefSeqGene project. On May 24, 2008 this sequence version replaced NM_000260.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The update from NM_00260.1 to NM_00260.2 was intended to only modify select nucleotide residues that did not match the reference genome; however, the update mistakenly represented use of an alternate splice donor and splice acceptor site. NM_00260.2 was subsequently updated to version 3 to represent the original exon and splice site combination. The difference between NM_000260.1 and NM_000260.3 involve single residue changes to match the reference genome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U39226.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGATTC TTCAGCAGGG GGACCATGTG TGGATGGACC TGAGATTGGG GCAGGAGTTC 
GACGTGCCCA TCGGGGCGGT GGTGAAGCTC TGCGACTCTG GGCAGGTCCA GGTGGTGGAT 
GATGAAGACA ATGAACACTG GATCTCTCCG CAGAACGCAA CGCACATCAA GCCTATGCAC 
CCCACGTCGG TCCACGGCGT GGAGGACATG ATCCGCCTGG GGGACCTCAA CGAGGCGGGC 
ATCTTGCGCA ACCTGCTTAT CCGCTACCGG GACCACCTCA TCTACACGTA TACGGGCTCC 
ATCCTGGTGG CTGTGAACCC CTACCAGCTG CTCTCCATCT ACTCGCCAGA GCACATCCGC 
CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAC 
TGCTACTTCA ACATGAAACG CAACAGCCGA GACCAGTGCT GCATCATCAG TGGGGAATCT 
GGGGCCGGGA AGACGGAGAG CACAAAGCTG ATCCTGCAGT TCCTGGCAGC CATCAGTGGG 
CAGCACTCGT GGATTGAGCA GCAGGTCTTG GAGGCCACCC CCATTCTGGA AGCATTTGGG 
AATGCCAAGA CCATCCGCAA TGACAACTCA AGCCGTTTCG GAAAGTACAT CGACATCCAC 
TTCAACAAGC GGGGCGCCAT CGAGGGCGCG AAGATTGAGC AGTACCTGCT GGAAAAGTCA 
CGTGTCTGTC GCCAGGCCCT GGATGAAAGG AACTACCACG TGTTCTACTG CATGCTGGAG 
GGTATGAGTG AGGATCAGAA GAAGAAGCTG GGCTTGGGCC AGGCCTCTGA CTACAACTAC 
TTGGCCATGG GTAACTGCAT AACCTGTGAG GGCCGGGTGG ACAGCCAGGA GTACGCCAAC 
ATCCGCTCCG CCATGAAGGT GCTCATGTTC ACTGACACCG AGAACTGGGA GATCTCGAAG 
CTCCTGGCTG CCATCCTGCA CCTGGGCAAC CTGCAGTATG AGGCACGCAC ATTTGAAAAC 
CTGGATGCCT GTGAGGTTCT CTTCTCCCCA TCGCTGGCCA CAGCTGCATC CCTGCTTGAG 
GTGAACCCCC CAGACCTGAT GAGCTGCCTG ACTAGCCGCA CCCTCATCAC CCGCGGGGAG 
ACGGTGTCCA CCCCACTGAG CAGGGAACAG GCACTGGACG TGCGCGACGC CTTCGTAAAG 
GGGATCTACG GGCGGCTGTT CGTGTGGATT GTGGACAAGA TCAACGCAGC AATTTACAAG 
CCTCCCTCCC AGGATGTGAA GAACTCTCGC AGGTCCATCG GCCTCCTGGA CATCTTTGGG 
TTTGAGAACT TTGCTGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTCGC CAATGAGCAC 
CTGCAGCAGT TCTTTGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAATA TGACCTGGAG 
AGCATTGACT GGCTGCACAT CGAGTTCACT GACAACCAGG ATGCCCTGGA CATGATTGCC 
AACAAGCCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACA 
GACACCACCA TGTTACACAA GCTGAACTCC CAGCACAAGC TCAACGCCAA CTACATCCCC 
CCCAAGAACA ACCATGAGAC CCAGTTTGGC ATCAACCATT TTGCAGGCAT CGTCTACTAT 
GAGACCCAAG GCTTCCTGGA GAAGAACCGA GACACCCTGC ATGGGGACAT TATCCAGCTG 
GTCCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT CGCCATGGGC 
GCCGAGACCA GGAAGCGCTC GCCCACACTT AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG 
CTGATGCGCA CGCTGGGTGC CTGCCAGCCC TTCTTTGTGC GATGCATCAA GCCCAATGAG 
TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGCGTGC GCCAGCTGCG GTACTCAGGA 
ATGATGGAGA CCATCCGAAT CCGCCGAGCT GGCTACCCCA TCCGCTACAG CTTCGTAGAG 
TTTGTGGAGC GGTACCGTGT GCTGCTGCCA GGTGTGAAGC CGGCCTACAA GCAGGGCGAC 
CTCCGCGGGA CTTGCCAGCG CATGGCTGAG GCTGTGCTGG GCACCCACGA TGACTGGCAG 
ATAGGCAAAA CCAAGATCTT TCTGAAGGAC CACCATGACA TGCTGCTGGA AGTGGAGCGG 
GACAAAGCCA TCACCGACAG AGTCATCCTC CTTCAGAAAG TCATCCGGGG ATTCAAAGAC 
AGGTCTAACT TTCTGAAGCT GAAGAACGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGT 
CACAACTGTA GGAAGAACTA CGGGCTGATG CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG 
CACCGCTCCC GGAAGCTGCA CCAGCAGTAC CGCCTGGCCC GCCAGCGCAT CATCCAGTTC 
CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC AAGGCCTTCC GCCACCGCCT CTGGGCTGTG 
CTCACCGTGC AGGCCTATGC CCGGGGCATG ATCGCCCGCA GGCTGCACCA ACGCCTCAGG 
GCTGAGTATC TGTGGCGCCT CGAGGCTGAG AAAATGCGGC TGGCGGAGGA AGAGAAGCTT 
CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC 
CTGGCCCAGC TGGCTCGTGA GGACGCTGAG CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG 
CGGAAGAAGG AGCTCCTGGA GCAGATGGAA AGGGCCCGCC ATGAGCCTGT CAATCACTCA 
GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCAG GTGGCCTGCC AGGCCAGGAG 
GGCCAGGCAC CTAGTGGCTT TGAGGACCTG GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG 
GACCTGGATG CAGCCCTGCC CCTGCCTGAC GAGGATGAGG AGGACCTCTC TGAGTATAAA 
TTTGCCAAGT TCGCGGCCAC CTACTTCCAG GGGACAACCA CGCACTCCTA CACCCGGCGG 
CCACTCAAAC AGCCACTGCT CTACCATGAC GACGAGGGTG ACCAGCTGGC AGCCCTGGCG 
GTCTGGATCA CCATCCTCCG CTTCATGGGG GACCTCCCTG AGCCCAAGTA CCACACAGCC 
ATGAGTGATG GCAGTGAGAA GATCCCTGTG ATGACCAAGA TTTATGAGAC CCTGGGCAAG 
AAGACGTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG 
GGCCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCATT TGACTCTGAA AAAGAAGTCC 
AAGCTCACAG AGGAGGTGAC CAAGAGGCTG CATGACGGGG AGTCCACAGT GCAGGGCAAC 
AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATCGGCAAT 
GGCATCCTGC GGCCAGCACT CCGGGACGAG ATCTACTGCC AGATCAGCAA GCAGCTGACC 
CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATTC TCGTGTCTCT CTGCGTGGGC 
TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG TACCTGCGGA ACTTCATCCA CGGGGGCCCG 
CCCGGCTACG CCCCGTACTG TGAGGAGCGC CTGAGAAGGA CCTTTGTCAA TGGGACACGG 
ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC AATCATGTTG 
CCCGTGACAT TCATGGATGG GACCACCAAG ACCCTGCTGA CGGACTCGGC AACCACGGCC 
AAGGAGCTCT GCAACGCGCT GGCCGACAAG ATCTCTCTCA AGGACCGGTT CGGGTTCTCC 
CTCTACATTG CCCTGTTTGA CAAGGTGTCC TCCCTGGGCA GCGGCAGTGA CCACGTCATG 
GACGCCATCT CCCAGTGCGA GCAGTACGCC AAGGAGCAGG GCGCCCAGGA GCGCAACGCC 
CCCTGGAGGC TCTTCTTCCG CAAAGAGGTC TTCACGCCCT GGCACAGCCC CTCCGAGGAC 
AACGTGGCCA CCAACCTCAT CTACCAGCAG GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC 
AGGTGTGAGA AGGAGGACGA CCTGGCTGAG CTGGCCTCCC AGCAGTACTT TGTAGACTAT 
GGCTCTGAGA TGATCCTGGA GCGCCTCCTG AACCTCGTGC CCACCTACAT CCCCGACCGC 
GAGATCACGC CCCTGAAGAC GCTGGAGAAG TGGGCCCAGC TGGCCATCGC CGCCCACAAG 
AAGGGGATTT ATGCCCAGAG GAGAACTGAT GCCCAGAAGG TCAAAGAGGA TGTGGTCAGT 
TATGCCCGCT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ATGAAGCCTA CAAATTCTCA 
GGCCCCAGTC TCCCCAAGAA CGACGTCATC GTGGCCGTCA ACTGGACGGG TGTGTACTTT 
GTGGATGAGC AGGAGCAGGT ACTTCTGGAG CTGTCCTTCC CAGAGATCAT GGCCGTGTCC 
AGCAGCAGGG AGTGCCGTGT CTGGCTCTCA CTGGGCTGCT CTGATCTTGG CTGTGCTGCG 
CCTCACTCAG GCTGGGCAGG ACTGACCCCG GCGGGGCCCT GTTCTCCGTG TTGGTCCTGC 
AGGGGAGCGA AAACGACGGC CCCCAGCTTC ACGCTGGCCA CCATCAAGGG GGACGAATAC 
ACCTTCACCT CCAGCAATGC TGAGGACATT CGTGACCTGG TGGTCACCTT CCTAGAGGGG 
CTCCGGAAGA GATCTAAGTA TGTTGTGGCC CTGCAGGATA ACCCCAACCC CGCAGGCGAG 
GAGTCAGGCT TCCTCAGCTT TGCCAAGGGA GACCTCATCA TCCTGGACCA TGACACGGGC 
GAGCAGGTCA TGAACTCGGG CTGGGCCAAC GGCATCAATG AGAGGACCAA GCAGCGTGGG 
GACTTCCCCA CCGACAGTGT GTACGTCATG CCCACTGTCA CCATGCCACC GCGGGAGATT 
GTGGCCCTGG TCACCATGAC TCCCGATCAG AGGCAGGACG TTGTCCGGCT CTTGCAGCTG 
CGAACGGCGG AGCCCGAGGT GCGTGCCAAG CCCTACACGC TGGAGGAGTT TTCCTATGAC 
TACTTCAGGC CCCCACCCAA GCACACGCTG AGCCGTGTCA TGGTGTCCAA GGCCCGAGGC 
AAGGACCGGC TGTGGAGCCA CACGCGGGAA CCGCTCAAGC AGGCGCTGCT CAAGAAGCTC 
CTGGGCAGTG AGGAGCTCTC GCAGGAGGCC TGCCTGGCCT TCATTGCTGT GCTCAAGTAC 
ATGGGCGACT ACCCGTCCAA GAGGACACGC TCCGTCAACG AGCTCACCGA CCAGATCTTT 
GAGGGTCCCC TGAAAGCCGA GCCCCTGAAG GACGAGGCAT ATGTGCAGAT CCTGAAGCAG 
CTGACCGACA ACCACATCAG GTACAGCGAG GAGCGGGGTT GGGAGCTGCT CTGGCTGTGC 
ACGGGCCTTT TCCCACCCAG CAACATCCTC CTGCCCCACG TGCAGCGCTT CCTGCAGTCC 
CGAAAGCACT GCCCACTCGC CATCGACTGC CTGCAACGGC TCCAGAAAGC CCTGAGAAAC 
GGGTCCCGGA AGTACCCTCC GCACCTGGTG GAGGTGGAGG CCATCCAGCA CAAGACCACC 
CAGATTTTCC ACAAAGTCTA CTTCCCTGAT GACACTGACG AGGCCTTCGA AGTGGAGTCC 
AGCACCAAGG CCAAGGACTT CTGCCAGAAC ATCGCCACCA GGCTGCTCCT CAAGTCCTCA 
GAGGGATTCA GCCTCTTTGT CAAAATTGCA GACAAGGTCC TCAGCGTTCC TGAGAATGAC 
TTCTTCTTTG ACTTTGTTCG ACACTTGACA GACTGGATAA AGAAAGCTCG GCCCATCAAG 
GACGGAATTG TGCCCTCACT CACCTACCAG GTGTTCTTCA TGAAGAAGCT GTGGACCACC 
ACGGTGCCAG GGAAGGATCC CATGGCCGAT TCCATCTTCC ACTATTACCA GGAGTTGCCC 
AAGTATCTCC GAGGCTACCA CAAGTGCACG CGGGAGGAGG TGCTGCAGCT GGGGGCGCTG 
ATCTACAGGG TCAAGTTCGA GGAGGACAAG TCCTACTTCC CCAGCATCCC CAAGCTGCTG 
CGGGAGCTGG TGCCCCAGGA CCTTATCCGG CAGGTCTCAC CTGATGACTG GAAGCGGTCC 
ATCGTCGCCT ACTTCAACAA GCACGCAGGG AAGTCCAAGG AGGAGGCCAA GCTGGCCTTC 
CTGAAGCTCA TCTTCAAGTG GCCCACCTTT GGCTCAGCCT TCTTCGAGGT GAAGCAAACT 
ACGGAGCCAA ACTTCCCTGA GATCCTCCTA ATTGCCATCA ACAAGTATGG GGTCAGCCTC 
ATCGATCCCA AAACGAAGGA TATCCTCACC ACTCATCCCT TCACCAAGAT CTCCAACTGG 
AGCAGCGGCA ACACCTACTT CCACATCACC ATTGGGAACT TGGTGCGCGG GAGCAAACTG 
CTCTGCGAGA CGTCACTGGG CTACAAGATG GATGACCTCC TGACTTCCTA CATTAGCCAG 
ATGCTCACAG CCATGAGCAA ACAGCGGGGC TCCAGGAGCG GCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000251.3
CDS	273..6920
Translation	MVILQQGDHVWMDLRLGQEFDVPIGAVVKLCDSGQVQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNSRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQALDERNYHVFYCMLEGMSEDQKKKLGLGQASDYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHLGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVDKINAAIYKPPSQDVKNSRRSIGLLDIFGFENFAVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQDALDMIANKPMNIISLIDEESKFPKGTDTTMLHKLNSQHKLNANYIPPKNNHETQFGINHFAGIVYYETQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRRAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLKLKNAATLIQRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLHQQYRLARQRIIQFQARCRAYLVRKAFRHRLWAVLTVQAYARGMIARRLHQRLRAEYLWRLEAEKMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEAARRKKELLEQMERARHEPVNHSDMVDKMFGFLGTSGGLPGQEGQAPSGFEDLERGRREMVEEDLDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGEAQLPEGQKKSSVRHKLVHLTLKKKSKLTEEVTKRLHDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTAKELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHSPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLNLVPTYIPDREITPLKTLEKWAQLAIAAHKKGIYAQRRTDAQKVKEDVVSYARFKWPLLFSRFYEAYKFSGPSLPKNDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRECRVWLSLGCSDLGCAAPHSGWAGLTPAGPCSPCWSCRGAKTTAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDSVYVMPTVTMPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKLLGSEELSQEACLAFIAVLKYMGDYPSKRTRSVNELTDQIFEGPLKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIATRLLLKSSEGFSLFVKIADKVLSVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKQTTEPNFPEILLIAINKYGVSLIDPKTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRGSRSGK

Target ORF information:

RefSeq Version	NM_000260.3
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VIIA (MYO7A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000260.3

ORF Insert Sequence:

ATGGTGATTC TTCAGCAGGG GGACCATGTG TGGATGGACC TGAGATTGGG GCAGGAGTTC 
GACGTGCCCA TCGGGGCGGT GGTGAAGCTC TGCGACTCTG GGCAGGTCCA GGTGGTGGAT 
GATGAAGACA ATGAACACTG GATCTCTCCG CAGAACGCAA CGCACATCAA GCCTATGCAC 
CCCACGTCGG TCCACGGCGT GGAGGACATG ATCCGCCTGG GGGACCTCAA CGAGGCGGGC 
ATCTTGCGCA ACCTGCTTAT CCGCTACCGG GACCACCTCA TCTACACGTA TACGGGCTCC 
ATCCTGGTGG CTGTGAACCC CTACCAGCTG CTCTCCATCT ACTCGCCAGA GCACATCCGC 
CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAC 
TGCTACTTCA ACATGAAACG CAACAGCCGA GACCAGTGCT GCATCATCAG TGGGGAATCT 
GGGGCCGGGA AGACGGAGAG CACAAAGCTG ATCCTGCAGT TCCTGGCAGC CATCAGTGGG 
CAGCACTCGT GGATTGAGCA GCAGGTCTTG GAGGCCACCC CCATTCTGGA AGCATTTGGG 
AATGCCAAGA CCATCCGCAA TGACAACTCA AGCCGTTTCG GAAAGTACAT CGACATCCAC 
TTCAACAAGC GGGGCGCCAT CGAGGGCGCG AAGATTGAGC AGTACCTGCT GGAAAAGTCA 
CGTGTCTGTC GCCAGGCCCT GGATGAAAGG AACTACCACG TGTTCTACTG CATGCTGGAG 
GGTATGAGTG AGGATCAGAA GAAGAAGCTG GGCTTGGGCC AGGCCTCTGA CTACAACTAC 
TTGGCCATGG GTAACTGCAT AACCTGTGAG GGCCGGGTGG ACAGCCAGGA GTACGCCAAC 
ATCCGCTCCG CCATGAAGGT GCTCATGTTC ACTGACACCG AGAACTGGGA GATCTCGAAG 
CTCCTGGCTG CCATCCTGCA CCTGGGCAAC CTGCAGTATG AGGCACGCAC ATTTGAAAAC 
CTGGATGCCT GTGAGGTTCT CTTCTCCCCA TCGCTGGCCA CAGCTGCATC CCTGCTTGAG 
GTGAACCCCC CAGACCTGAT GAGCTGCCTG ACTAGCCGCA CCCTCATCAC CCGCGGGGAG 
ACGGTGTCCA CCCCACTGAG CAGGGAACAG GCACTGGACG TGCGCGACGC CTTCGTAAAG 
GGGATCTACG GGCGGCTGTT CGTGTGGATT GTGGACAAGA TCAACGCAGC AATTTACAAG 
CCTCCCTCCC AGGATGTGAA GAACTCTCGC AGGTCCATCG GCCTCCTGGA CATCTTTGGG 
TTTGAGAACT TTGCTGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTCGC CAATGAGCAC 
CTGCAGCAGT TCTTTGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAATA TGACCTGGAG 
AGCATTGACT GGCTGCACAT CGAGTTCACT GACAACCAGG ATGCCCTGGA CATGATTGCC 
AACAAGCCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACA 
GACACCACCA TGTTACACAA GCTGAACTCC CAGCACAAGC TCAACGCCAA CTACATCCCC 
CCCAAGAACA ACCATGAGAC CCAGTTTGGC ATCAACCATT TTGCAGGCAT CGTCTACTAT 
GAGACCCAAG GCTTCCTGGA GAAGAACCGA GACACCCTGC ATGGGGACAT TATCCAGCTG 
GTCCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT CGCCATGGGC 
GCCGAGACCA GGAAGCGCTC GCCCACACTT AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG 
CTGATGCGCA CGCTGGGTGC CTGCCAGCCC TTCTTTGTGC GATGCATCAA GCCCAATGAG 
TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGCGTGC GCCAGCTGCG GTACTCAGGA 
ATGATGGAGA CCATCCGAAT CCGCCGAGCT GGCTACCCCA TCCGCTACAG CTTCGTAGAG 
TTTGTGGAGC GGTACCGTGT GCTGCTGCCA GGTGTGAAGC CGGCCTACAA GCAGGGCGAC 
CTCCGCGGGA CTTGCCAGCG CATGGCTGAG GCTGTGCTGG GCACCCACGA TGACTGGCAG 
ATAGGCAAAA CCAAGATCTT TCTGAAGGAC CACCATGACA TGCTGCTGGA AGTGGAGCGG 
GACAAAGCCA TCACCGACAG AGTCATCCTC CTTCAGAAAG TCATCCGGGG ATTCAAAGAC 
AGGTCTAACT TTCTGAAGCT GAAGAACGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGT 
CACAACTGTA GGAAGAACTA CGGGCTGATG CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG 
CACCGCTCCC GGAAGCTGCA CCAGCAGTAC CGCCTGGCCC GCCAGCGCAT CATCCAGTTC 
CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC AAGGCCTTCC GCCACCGCCT CTGGGCTGTG 
CTCACCGTGC AGGCCTATGC CCGGGGCATG ATCGCCCGCA GGCTGCACCA ACGCCTCAGG 
GCTGAGTATC TGTGGCGCCT CGAGGCTGAG AAAATGCGGC TGGCGGAGGA AGAGAAGCTT 
CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC 
CTGGCCCAGC TGGCTCGTGA GGACGCTGAG CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG 
CGGAAGAAGG AGCTCCTGGA GCAGATGGAA AGGGCCCGCC ATGAGCCTGT CAATCACTCA 
GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCAG GTGGCCTGCC AGGCCAGGAG 
GGCCAGGCAC CTAGTGGCTT TGAGGACCTG GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG 
GACCTGGATG CAGCCCTGCC CCTGCCTGAC GAGGATGAGG AGGACCTCTC TGAGTATAAA 
TTTGCCAAGT TCGCGGCCAC CTACTTCCAG GGGACAACCA CGCACTCCTA CACCCGGCGG 
CCACTCAAAC AGCCACTGCT CTACCATGAC GACGAGGGTG ACCAGCTGGC AGCCCTGGCG 
GTCTGGATCA CCATCCTCCG CTTCATGGGG GACCTCCCTG AGCCCAAGTA CCACACAGCC 
ATGAGTGATG GCAGTGAGAA GATCCCTGTG ATGACCAAGA TTTATGAGAC CCTGGGCAAG 
AAGACGTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG 
GGCCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCATT TGACTCTGAA AAAGAAGTCC 
AAGCTCACAG AGGAGGTGAC CAAGAGGCTG CATGACGGGG AGTCCACAGT GCAGGGCAAC 
AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATCGGCAAT 
GGCATCCTGC GGCCAGCACT CCGGGACGAG ATCTACTGCC AGATCAGCAA GCAGCTGACC 
CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATTC TCGTGTCTCT CTGCGTGGGC 
TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG TACCTGCGGA ACTTCATCCA CGGGGGCCCG 
CCCGGCTACG CCCCGTACTG TGAGGAGCGC CTGAGAAGGA CCTTTGTCAA TGGGACACGG 
ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC AATCATGTTG 
CCCGTGACAT TCATGGATGG GACCACCAAG ACCCTGCTGA CGGACTCGGC AACCACGGCC 
AAGGAGCTCT GCAACGCGCT GGCCGACAAG ATCTCTCTCA AGGACCGGTT CGGGTTCTCC 
CTCTACATTG CCCTGTTTGA CAAGGTGTCC TCCCTGGGCA GCGGCAGTGA CCACGTCATG 
GACGCCATCT CCCAGTGCGA GCAGTACGCC AAGGAGCAGG GCGCCCAGGA GCGCAACGCC 
CCCTGGAGGC TCTTCTTCCG CAAAGAGGTC TTCACGCCCT GGCACAGCCC CTCCGAGGAC 
AACGTGGCCA CCAACCTCAT CTACCAGCAG GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC 
AGGTGTGAGA AGGAGGACGA CCTGGCTGAG CTGGCCTCCC AGCAGTACTT TGTAGACTAT 
GGCTCTGAGA TGATCCTGGA GCGCCTCCTG AACCTCGTGC CCACCTACAT CCCCGACCGC 
GAGATCACGC CCCTGAAGAC GCTGGAGAAG TGGGCCCAGC TGGCCATCGC CGCCCACAAG 
AAGGGGATTT ATGCCCAGAG GAGAACTGAT GCCCAGAAGG TCAAAGAGGA TGTGGTCAGT 
TATGCCCGCT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ATGAAGCCTA CAAATTCTCA 
GGCCCCAGTC TCCCCAAGAA CGACGTCATC GTGGCCGTCA ACTGGACGGG TGTGTACTTT 
GTGGATGAGC AGGAGCAGGT ACTTCTGGAG CTGTCCTTCC CAGAGATCAT GGCCGTGTCC 
AGCAGCAGGG AGTGCCGTGT CTGGCTCTCA CTGGGCTGCT CTGATCTTGG CTGTGCTGCG 
CCTCACTCAG GCTGGGCAGG ACTGACCCCG GCGGGGCCCT GTTCTCCGTG TTGGTCCTGC 
AGGGGAGCGA AAACGACGGC CCCCAGCTTC ACGCTGGCCA CCATCAAGGG GGACGAATAC 
ACCTTCACCT CCAGCAATGC TGAGGACATT CGTGACCTGG TGGTCACCTT CCTAGAGGGG 
CTCCGGAAGA GATCTAAGTA TGTTGTGGCC CTGCAGGATA ACCCCAACCC CGCAGGCGAG 
GAGTCAGGCT TCCTCAGCTT TGCCAAGGGA GACCTCATCA TCCTGGACCA TGACACGGGC 
GAGCAGGTCA TGAACTCGGG CTGGGCCAAC GGCATCAATG AGAGGACCAA GCAGCGTGGG 
GACTTCCCCA CCGACAGTGT GTACGTCATG CCCACTGTCA CCATGCCACC GCGGGAGATT 
GTGGCCCTGG TCACCATGAC TCCCGATCAG AGGCAGGACG TTGTCCGGCT CTTGCAGCTG 
CGAACGGCGG AGCCCGAGGT GCGTGCCAAG CCCTACACGC TGGAGGAGTT TTCCTATGAC 
TACTTCAGGC CCCCACCCAA GCACACGCTG AGCCGTGTCA TGGTGTCCAA GGCCCGAGGC 
AAGGACCGGC TGTGGAGCCA CACGCGGGAA CCGCTCAAGC AGGCGCTGCT CAAGAAGCTC 
CTGGGCAGTG AGGAGCTCTC GCAGGAGGCC TGCCTGGCCT TCATTGCTGT GCTCAAGTAC 
ATGGGCGACT ACCCGTCCAA GAGGACACGC TCCGTCAACG AGCTCACCGA CCAGATCTTT 
GAGGGTCCCC TGAAAGCCGA GCCCCTGAAG GACGAGGCAT ATGTGCAGAT CCTGAAGCAG 
CTGACCGACA ACCACATCAG GTACAGCGAG GAGCGGGGTT GGGAGCTGCT CTGGCTGTGC 
ACGGGCCTTT TCCCACCCAG CAACATCCTC CTGCCCCACG TGCAGCGCTT CCTGCAGTCC 
CGAAAGCACT GCCCACTCGC CATCGACTGC CTGCAACGGC TCCAGAAAGC CCTGAGAAAC 
GGGTCCCGGA AGTACCCTCC GCACCTGGTG GAGGTGGAGG CCATCCAGCA CAAGACCACC 
CAGATTTTCC ACAAAGTCTA CTTCCCTGAT GACACTGACG AGGCCTTCGA AGTGGAGTCC 
AGCACCAAGG CCAAGGACTT CTGCCAGAAC ATCGCCACCA GGCTGCTCCT CAAGTCCTCA 
GAGGGATTCA GCCTCTTTGT CAAAATTGCA GACAAGGTCC TCAGCGTTCC TGAGAATGAC 
TTCTTCTTTG ACTTTGTTCG ACACTTGACA GACTGGATAA AGAAAGCTCG GCCCATCAAG 
GACGGAATTG TGCCCTCACT CACCTACCAG GTGTTCTTCA TGAAGAAGCT GTGGACCACC 
ACGGTGCCAG GGAAGGATCC CATGGCCGAT TCCATCTTCC ACTATTACCA GGAGTTGCCC 
AAGTATCTCC GAGGCTACCA CAAGTGCACG CGGGAGGAGG TGCTGCAGCT GGGGGCGCTG 
ATCTACAGGG TCAAGTTCGA GGAGGACAAG TCCTACTTCC CCAGCATCCC CAAGCTGCTG 
CGGGAGCTGG TGCCCCAGGA CCTTATCCGG CAGGTCTCAC CTGATGACTG GAAGCGGTCC 
ATCGTCGCCT ACTTCAACAA GCACGCAGGG AAGTCCAAGG AGGAGGCCAA GCTGGCCTTC 
CTGAAGCTCA TCTTCAAGTG GCCCACCTTT GGCTCAGCCT TCTTCGAGGT GAAGCAAACT 
ACGGAGCCAA ACTTCCCTGA GATCCTCCTA ATTGCCATCA ACAAGTATGG GGTCAGCCTC 
ATCGATCCCA AAACGAAGGA TATCCTCACC ACTCATCCCT TCACCAAGAT CTCCAACTGG 
AGCAGCGGCA ACACCTACTT CCACATCACC ATTGGGAACT TGGTGCGCGG GAGCAAACTG 
CTCTGCGAGA CGTCACTGGG CTACAAGATG GATGACCTCC TGACTTCCTA CATTAGCCAG 
ATGCTCACAG CCATGAGCAA ACAGCGGGGC TCCAGGAGCG GCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYO7A ( NM_000260.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000251.3 Homo sapiens myosin VIIA (MYO7A), transcript variant 1, mRNA.

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CloneID	OHu27355
Clone ID Related Accession (Same CDS sequence)	NM_000260.3 , NM_000260.4
Accession Version	NM_000260.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	unconventional myosin-VIIa isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000752.4, U39226.1, U34227.1, U55208.1 and BG108466.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_000260.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The update from NM_00260.1 to NM_00260.2 was intended to only modify select nucleotide residues that did not match the reference genome; however, the update mistakenly represented use of an alternate splice donor and splice acceptor site. NM_00260.2 was subsequently updated to version 3 to represent the original exon and splice site combination. The difference between NM_000260.1 and NM_000260.3 involve single residue changes to match the reference genome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U39226.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000409709.9/ ENSP00000386331.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGATTC TTCAGCAGGG GGACCATGTG TGGATGGACC TGAGATTGGG GCAGGAGTTC 
GACGTGCCCA TCGGGGCGGT GGTGAAGCTC TGCGACTCTG GGCAGGTCCA GGTGGTGGAT 
GATGAAGACA ATGAACACTG GATCTCTCCG CAGAACGCAA CGCACATCAA GCCTATGCAC 
CCCACGTCGG TCCACGGCGT GGAGGACATG ATCCGCCTGG GGGACCTCAA CGAGGCGGGC 
ATCTTGCGCA ACCTGCTTAT CCGCTACCGG GACCACCTCA TCTACACGTA TACGGGCTCC 
ATCCTGGTGG CTGTGAACCC CTACCAGCTG CTCTCCATCT ACTCGCCAGA GCACATCCGC 
CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAC 
TGCTACTTCA ACATGAAACG CAACAGCCGA GACCAGTGCT GCATCATCAG TGGGGAATCT 
GGGGCCGGGA AGACGGAGAG CACAAAGCTG ATCCTGCAGT TCCTGGCAGC CATCAGTGGG 
CAGCACTCGT GGATTGAGCA GCAGGTCTTG GAGGCCACCC CCATTCTGGA AGCATTTGGG 
AATGCCAAGA CCATCCGCAA TGACAACTCA AGCCGTTTCG GAAAGTACAT CGACATCCAC 
TTCAACAAGC GGGGCGCCAT CGAGGGCGCG AAGATTGAGC AGTACCTGCT GGAAAAGTCA 
CGTGTCTGTC GCCAGGCCCT GGATGAAAGG AACTACCACG TGTTCTACTG CATGCTGGAG 
GGTATGAGTG AGGATCAGAA GAAGAAGCTG GGCTTGGGCC AGGCCTCTGA CTACAACTAC 
TTGGCCATGG GTAACTGCAT AACCTGTGAG GGCCGGGTGG ACAGCCAGGA GTACGCCAAC 
ATCCGCTCCG CCATGAAGGT GCTCATGTTC ACTGACACCG AGAACTGGGA GATCTCGAAG 
CTCCTGGCTG CCATCCTGCA CCTGGGCAAC CTGCAGTATG AGGCACGCAC ATTTGAAAAC 
CTGGATGCCT GTGAGGTTCT CTTCTCCCCA TCGCTGGCCA CAGCTGCATC CCTGCTTGAG 
GTGAACCCCC CAGACCTGAT GAGCTGCCTG ACTAGCCGCA CCCTCATCAC CCGCGGGGAG 
ACGGTGTCCA CCCCACTGAG CAGGGAACAG GCACTGGACG TGCGCGACGC CTTCGTAAAG 
GGGATCTACG GGCGGCTGTT CGTGTGGATT GTGGACAAGA TCAACGCAGC AATTTACAAG 
CCTCCCTCCC AGGATGTGAA GAACTCTCGC AGGTCCATCG GCCTCCTGGA CATCTTTGGG 
TTTGAGAACT TTGCTGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTCGC CAATGAGCAC 
CTGCAGCAGT TCTTTGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAATA TGACCTGGAG 
AGCATTGACT GGCTGCACAT CGAGTTCACT GACAACCAGG ATGCCCTGGA CATGATTGCC 
AACAAGCCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACA 
GACACCACCA TGTTACACAA GCTGAACTCC CAGCACAAGC TCAACGCCAA CTACATCCCC 
CCCAAGAACA ACCATGAGAC CCAGTTTGGC ATCAACCATT TTGCAGGCAT CGTCTACTAT 
GAGACCCAAG GCTTCCTGGA GAAGAACCGA GACACCCTGC ATGGGGACAT TATCCAGCTG 
GTCCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT CGCCATGGGC 
GCCGAGACCA GGAAGCGCTC GCCCACACTT AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG 
CTGATGCGCA CGCTGGGTGC CTGCCAGCCC TTCTTTGTGC GATGCATCAA GCCCAATGAG 
TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGCGTGC GCCAGCTGCG GTACTCAGGA 
ATGATGGAGA CCATCCGAAT CCGCCGAGCT GGCTACCCCA TCCGCTACAG CTTCGTAGAG 
TTTGTGGAGC GGTACCGTGT GCTGCTGCCA GGTGTGAAGC CGGCCTACAA GCAGGGCGAC 
CTCCGCGGGA CTTGCCAGCG CATGGCTGAG GCTGTGCTGG GCACCCACGA TGACTGGCAG 
ATAGGCAAAA CCAAGATCTT TCTGAAGGAC CACCATGACA TGCTGCTGGA AGTGGAGCGG 
GACAAAGCCA TCACCGACAG AGTCATCCTC CTTCAGAAAG TCATCCGGGG ATTCAAAGAC 
AGGTCTAACT TTCTGAAGCT GAAGAACGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGT 
CACAACTGTA GGAAGAACTA CGGGCTGATG CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG 
CACCGCTCCC GGAAGCTGCA CCAGCAGTAC CGCCTGGCCC GCCAGCGCAT CATCCAGTTC 
CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC AAGGCCTTCC GCCACCGCCT CTGGGCTGTG 
CTCACCGTGC AGGCCTATGC CCGGGGCATG ATCGCCCGCA GGCTGCACCA ACGCCTCAGG 
GCTGAGTATC TGTGGCGCCT CGAGGCTGAG AAAATGCGGC TGGCGGAGGA AGAGAAGCTT 
CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC 
CTGGCCCAGC TGGCTCGTGA GGACGCTGAG CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG 
CGGAAGAAGG AGCTCCTGGA GCAGATGGAA AGGGCCCGCC ATGAGCCTGT CAATCACTCA 
GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCAG GTGGCCTGCC AGGCCAGGAG 
GGCCAGGCAC CTAGTGGCTT TGAGGACCTG GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG 
GACCTGGATG CAGCCCTGCC CCTGCCTGAC GAGGATGAGG AGGACCTCTC TGAGTATAAA 
TTTGCCAAGT TCGCGGCCAC CTACTTCCAG GGGACAACCA CGCACTCCTA CACCCGGCGG 
CCACTCAAAC AGCCACTGCT CTACCATGAC GACGAGGGTG ACCAGCTGGC AGCCCTGGCG 
GTCTGGATCA CCATCCTCCG CTTCATGGGG GACCTCCCTG AGCCCAAGTA CCACACAGCC 
ATGAGTGATG GCAGTGAGAA GATCCCTGTG ATGACCAAGA TTTATGAGAC CCTGGGCAAG 
AAGACGTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG 
GGCCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCATT TGACTCTGAA AAAGAAGTCC 
AAGCTCACAG AGGAGGTGAC CAAGAGGCTG CATGACGGGG AGTCCACAGT GCAGGGCAAC 
AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATCGGCAAT 
GGCATCCTGC GGCCAGCACT CCGGGACGAG ATCTACTGCC AGATCAGCAA GCAGCTGACC 
CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATTC TCGTGTCTCT CTGCGTGGGC 
TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG TACCTGCGGA ACTTCATCCA CGGGGGCCCG 
CCCGGCTACG CCCCGTACTG TGAGGAGCGC CTGAGAAGGA CCTTTGTCAA TGGGACACGG 
ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC AATCATGTTG 
CCCGTGACAT TCATGGATGG GACCACCAAG ACCCTGCTGA CGGACTCGGC AACCACGGCC 
AAGGAGCTCT GCAACGCGCT GGCCGACAAG ATCTCTCTCA AGGACCGGTT CGGGTTCTCC 
CTCTACATTG CCCTGTTTGA CAAGGTGTCC TCCCTGGGCA GCGGCAGTGA CCACGTCATG 
GACGCCATCT CCCAGTGCGA GCAGTACGCC AAGGAGCAGG GCGCCCAGGA GCGCAACGCC 
CCCTGGAGGC TCTTCTTCCG CAAAGAGGTC TTCACGCCCT GGCACAGCCC CTCCGAGGAC 
AACGTGGCCA CCAACCTCAT CTACCAGCAG GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC 
AGGTGTGAGA AGGAGGACGA CCTGGCTGAG CTGGCCTCCC AGCAGTACTT TGTAGACTAT 
GGCTCTGAGA TGATCCTGGA GCGCCTCCTG AACCTCGTGC CCACCTACAT CCCCGACCGC 
GAGATCACGC CCCTGAAGAC GCTGGAGAAG TGGGCCCAGC TGGCCATCGC CGCCCACAAG 
AAGGGGATTT ATGCCCAGAG GAGAACTGAT GCCCAGAAGG TCAAAGAGGA TGTGGTCAGT 
TATGCCCGCT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ATGAAGCCTA CAAATTCTCA 
GGCCCCAGTC TCCCCAAGAA CGACGTCATC GTGGCCGTCA ACTGGACGGG TGTGTACTTT 
GTGGATGAGC AGGAGCAGGT ACTTCTGGAG CTGTCCTTCC CAGAGATCAT GGCCGTGTCC 
AGCAGCAGGG AGTGCCGTGT CTGGCTCTCA CTGGGCTGCT CTGATCTTGG CTGTGCTGCG 
CCTCACTCAG GCTGGGCAGG ACTGACCCCG GCGGGGCCCT GTTCTCCGTG TTGGTCCTGC 
AGGGGAGCGA AAACGACGGC CCCCAGCTTC ACGCTGGCCA CCATCAAGGG GGACGAATAC 
ACCTTCACCT CCAGCAATGC TGAGGACATT CGTGACCTGG TGGTCACCTT CCTAGAGGGG 
CTCCGGAAGA GATCTAAGTA TGTTGTGGCC CTGCAGGATA ACCCCAACCC CGCAGGCGAG 
GAGTCAGGCT TCCTCAGCTT TGCCAAGGGA GACCTCATCA TCCTGGACCA TGACACGGGC 
GAGCAGGTCA TGAACTCGGG CTGGGCCAAC GGCATCAATG AGAGGACCAA GCAGCGTGGG 
GACTTCCCCA CCGACAGTGT GTACGTCATG CCCACTGTCA CCATGCCACC GCGGGAGATT 
GTGGCCCTGG TCACCATGAC TCCCGATCAG AGGCAGGACG TTGTCCGGCT CTTGCAGCTG 
CGAACGGCGG AGCCCGAGGT GCGTGCCAAG CCCTACACGC TGGAGGAGTT TTCCTATGAC 
TACTTCAGGC CCCCACCCAA GCACACGCTG AGCCGTGTCA TGGTGTCCAA GGCCCGAGGC 
AAGGACCGGC TGTGGAGCCA CACGCGGGAA CCGCTCAAGC AGGCGCTGCT CAAGAAGCTC 
CTGGGCAGTG AGGAGCTCTC GCAGGAGGCC TGCCTGGCCT TCATTGCTGT GCTCAAGTAC 
ATGGGCGACT ACCCGTCCAA GAGGACACGC TCCGTCAACG AGCTCACCGA CCAGATCTTT 
GAGGGTCCCC TGAAAGCCGA GCCCCTGAAG GACGAGGCAT ATGTGCAGAT CCTGAAGCAG 
CTGACCGACA ACCACATCAG GTACAGCGAG GAGCGGGGTT GGGAGCTGCT CTGGCTGTGC 
ACGGGCCTTT TCCCACCCAG CAACATCCTC CTGCCCCACG TGCAGCGCTT CCTGCAGTCC 
CGAAAGCACT GCCCACTCGC CATCGACTGC CTGCAACGGC TCCAGAAAGC CCTGAGAAAC 
GGGTCCCGGA AGTACCCTCC GCACCTGGTG GAGGTGGAGG CCATCCAGCA CAAGACCACC 
CAGATTTTCC ACAAAGTCTA CTTCCCTGAT GACACTGACG AGGCCTTCGA AGTGGAGTCC 
AGCACCAAGG CCAAGGACTT CTGCCAGAAC ATCGCCACCA GGCTGCTCCT CAAGTCCTCA 
GAGGGATTCA GCCTCTTTGT CAAAATTGCA GACAAGGTCC TCAGCGTTCC TGAGAATGAC 
TTCTTCTTTG ACTTTGTTCG ACACTTGACA GACTGGATAA AGAAAGCTCG GCCCATCAAG 
GACGGAATTG TGCCCTCACT CACCTACCAG GTGTTCTTCA TGAAGAAGCT GTGGACCACC 
ACGGTGCCAG GGAAGGATCC CATGGCCGAT TCCATCTTCC ACTATTACCA GGAGTTGCCC 
AAGTATCTCC GAGGCTACCA CAAGTGCACG CGGGAGGAGG TGCTGCAGCT GGGGGCGCTG 
ATCTACAGGG TCAAGTTCGA GGAGGACAAG TCCTACTTCC CCAGCATCCC CAAGCTGCTG 
CGGGAGCTGG TGCCCCAGGA CCTTATCCGG CAGGTCTCAC CTGATGACTG GAAGCGGTCC 
ATCGTCGCCT ACTTCAACAA GCACGCAGGG AAGTCCAAGG AGGAGGCCAA GCTGGCCTTC 
CTGAAGCTCA TCTTCAAGTG GCCCACCTTT GGCTCAGCCT TCTTCGAGGT GAAGCAAACT 
ACGGAGCCAA ACTTCCCTGA GATCCTCCTA ATTGCCATCA ACAAGTATGG GGTCAGCCTC 
ATCGATCCCA AAACGAAGGA TATCCTCACC ACTCATCCCT TCACCAAGAT CTCCAACTGG 
AGCAGCGGCA ACACCTACTT CCACATCACC ATTGGGAACT TGGTGCGCGG GAGCAAACTG 
CTCTGCGAGA CGTCACTGGG CTACAAGATG GATGACCTCC TGACTTCCTA CATTAGCCAG 
ATGCTCACAG CCATGAGCAA ACAGCGGGGC TCCAGGAGCG GCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000251.3
CDS	291..6938
Translation	MVILQQGDHVWMDLRLGQEFDVPIGAVVKLCDSGQVQVVDDEDNEHWISPQNATHIKPMHPTSVHGVEDMIRLGDLNEAGILRNLLIRYRDHLIYTYTGSILVAVNPYQLLSIYSPEHIRQYTNKKIGEMPPHIFAIADNCYFNMKRNSRDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQALDERNYHVFYCMLEGMSEDQKKKLGLGQASDYNYLAMGNCITCEGRVDSQEYANIRSAMKVLMFTDTENWEISKLLAAILHLGNLQYEARTFENLDACEVLFSPSLATAASLLEVNPPDLMSCLTSRTLITRGETVSTPLSREQALDVRDAFVKGIYGRLFVWIVDKINAAIYKPPSQDVKNSRRSIGLLDIFGFENFAVNSFEQLCINFANEHLQQFFVRHVFKLEQEEYDLESIDWLHIEFTDNQDALDMIANKPMNIISLIDEESKFPKGTDTTMLHKLNSQHKLNANYIPPKNNHETQFGINHFAGIVYYETQGFLEKNRDTLHGDIIQLVHSSRNKFIKQIFQADVAMGAETRKRSPTLSSQFKRSLELLMRTLGACQPFFVRCIKPNEFKKPMLFDRHLCVRQLRYSGMMETIRIRRAGYPIRYSFVEFVERYRVLLPGVKPAYKQGDLRGTCQRMAEAVLGTHDDWQIGKTKIFLKDHHDMLLEVERDKAITDRVILLQKVIRGFKDRSNFLKLKNAATLIQRHWRGHNCRKNYGLMRLGFLRLQALHRSRKLHQQYRLARQRIIQFQARCRAYLVRKAFRHRLWAVLTVQAYARGMIARRLHQRLRAEYLWRLEAEKMRLAEEEKLRKEMSAKKAKEEAERKHQERLAQLAREDAERELKEKEAARRKKELLEQMERARHEPVNHSDMVDKMFGFLGTSGGLPGQEGQAPSGFEDLERGRREMVEEDLDAALPLPDEDEEDLSEYKFAKFAATYFQGTTTHSYTRRPLKQPLLYHDDEGDQLAALAVWITILRFMGDLPEPKYHTAMSDGSEKIPVMTKIYETLGKKTYKRELQALQGEGEAQLPEGQKKSSVRHKLVHLTLKKKSKLTEEVTKRLHDGESTVQGNSMLEDRPTSNLEKLHFIIGNGILRPALRDEIYCQISKQLTHNPSKSSYARGWILVSLCVGCFAPSEKFVKYLRNFIHGGPPGYAPYCEERLRRTFVNGTRTQPPSWLELQATKSKKPIMLPVTFMDGTTKTLLTDSATTAKELCNALADKISLKDRFGFSLYIALFDKVSSLGSGSDHVMDAISQCEQYAKEQGAQERNAPWRLFFRKEVFTPWHSPSEDNVATNLIYQQVVRGVKFGEYRCEKEDDLAELASQQYFVDYGSEMILERLLNLVPTYIPDREITPLKTLEKWAQLAIAAHKKGIYAQRRTDAQKVKEDVVSYARFKWPLLFSRFYEAYKFSGPSLPKNDVIVAVNWTGVYFVDEQEQVLLELSFPEIMAVSSSRECRVWLSLGCSDLGCAAPHSGWAGLTPAGPCSPCWSCRGAKTTAPSFTLATIKGDEYTFTSSNAEDIRDLVVTFLEGLRKRSKYVVALQDNPNPAGEESGFLSFAKGDLIILDHDTGEQVMNSGWANGINERTKQRGDFPTDSVYVMPTVTMPPREIVALVTMTPDQRQDVVRLLQLRTAEPEVRAKPYTLEEFSYDYFRPPPKHTLSRVMVSKARGKDRLWSHTREPLKQALLKKLLGSEELSQEACLAFIAVLKYMGDYPSKRTRSVNELTDQIFEGPLKAEPLKDEAYVQILKQLTDNHIRYSEERGWELLWLCTGLFPPSNILLPHVQRFLQSRKHCPLAIDCLQRLQKALRNGSRKYPPHLVEVEAIQHKTTQIFHKVYFPDDTDEAFEVESSTKAKDFCQNIATRLLLKSSEGFSLFVKIADKVLSVPENDFFFDFVRHLTDWIKKARPIKDGIVPSLTYQVFFMKKLWTTTVPGKDPMADSIFHYYQELPKYLRGYHKCTREEVLQLGALIYRVKFEEDKSYFPSIPKLLRELVPQDLIRQVSPDDWKRSIVAYFNKHAGKSKEEAKLAFLKLIFKWPTFGSAFFEVKQTTEPNFPEILLIAINKYGVSLIDPKTKDILTTHPFTKISNWSSGNTYFHITIGNLVRGSKLLCETSLGYKMDDLLTSYISQMLTAMSKQRGSRSGK

Target ORF information:

RefSeq Version	NM_000260.4
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin VIIA (MYO7A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000260.4

ORF Insert Sequence:

ATGGTGATTC TTCAGCAGGG GGACCATGTG TGGATGGACC TGAGATTGGG GCAGGAGTTC 
GACGTGCCCA TCGGGGCGGT GGTGAAGCTC TGCGACTCTG GGCAGGTCCA GGTGGTGGAT 
GATGAAGACA ATGAACACTG GATCTCTCCG CAGAACGCAA CGCACATCAA GCCTATGCAC 
CCCACGTCGG TCCACGGCGT GGAGGACATG ATCCGCCTGG GGGACCTCAA CGAGGCGGGC 
ATCTTGCGCA ACCTGCTTAT CCGCTACCGG GACCACCTCA TCTACACGTA TACGGGCTCC 
ATCCTGGTGG CTGTGAACCC CTACCAGCTG CTCTCCATCT ACTCGCCAGA GCACATCCGC 
CAGTATACCA ACAAGAAGAT TGGGGAGATG CCCCCCCACA TCTTTGCCAT TGCTGACAAC 
TGCTACTTCA ACATGAAACG CAACAGCCGA GACCAGTGCT GCATCATCAG TGGGGAATCT 
GGGGCCGGGA AGACGGAGAG CACAAAGCTG ATCCTGCAGT TCCTGGCAGC CATCAGTGGG 
CAGCACTCGT GGATTGAGCA GCAGGTCTTG GAGGCCACCC CCATTCTGGA AGCATTTGGG 
AATGCCAAGA CCATCCGCAA TGACAACTCA AGCCGTTTCG GAAAGTACAT CGACATCCAC 
TTCAACAAGC GGGGCGCCAT CGAGGGCGCG AAGATTGAGC AGTACCTGCT GGAAAAGTCA 
CGTGTCTGTC GCCAGGCCCT GGATGAAAGG AACTACCACG TGTTCTACTG CATGCTGGAG 
GGTATGAGTG AGGATCAGAA GAAGAAGCTG GGCTTGGGCC AGGCCTCTGA CTACAACTAC 
TTGGCCATGG GTAACTGCAT AACCTGTGAG GGCCGGGTGG ACAGCCAGGA GTACGCCAAC 
ATCCGCTCCG CCATGAAGGT GCTCATGTTC ACTGACACCG AGAACTGGGA GATCTCGAAG 
CTCCTGGCTG CCATCCTGCA CCTGGGCAAC CTGCAGTATG AGGCACGCAC ATTTGAAAAC 
CTGGATGCCT GTGAGGTTCT CTTCTCCCCA TCGCTGGCCA CAGCTGCATC CCTGCTTGAG 
GTGAACCCCC CAGACCTGAT GAGCTGCCTG ACTAGCCGCA CCCTCATCAC CCGCGGGGAG 
ACGGTGTCCA CCCCACTGAG CAGGGAACAG GCACTGGACG TGCGCGACGC CTTCGTAAAG 
GGGATCTACG GGCGGCTGTT CGTGTGGATT GTGGACAAGA TCAACGCAGC AATTTACAAG 
CCTCCCTCCC AGGATGTGAA GAACTCTCGC AGGTCCATCG GCCTCCTGGA CATCTTTGGG 
TTTGAGAACT TTGCTGTGAA CAGCTTTGAG CAGCTCTGCA TCAACTTCGC CAATGAGCAC 
CTGCAGCAGT TCTTTGTGCG GCACGTGTTC AAGCTGGAGC AGGAGGAATA TGACCTGGAG 
AGCATTGACT GGCTGCACAT CGAGTTCACT GACAACCAGG ATGCCCTGGA CATGATTGCC 
AACAAGCCCA TGAACATCAT CTCCCTCATC GATGAGGAGA GCAAGTTCCC CAAGGGCACA 
GACACCACCA TGTTACACAA GCTGAACTCC CAGCACAAGC TCAACGCCAA CTACATCCCC 
CCCAAGAACA ACCATGAGAC CCAGTTTGGC ATCAACCATT TTGCAGGCAT CGTCTACTAT 
GAGACCCAAG GCTTCCTGGA GAAGAACCGA GACACCCTGC ATGGGGACAT TATCCAGCTG 
GTCCACTCCT CCAGGAACAA GTTCATCAAG CAGATCTTCC AGGCCGATGT CGCCATGGGC 
GCCGAGACCA GGAAGCGCTC GCCCACACTT AGCAGCCAGT TCAAGCGGTC ACTGGAGCTG 
CTGATGCGCA CGCTGGGTGC CTGCCAGCCC TTCTTTGTGC GATGCATCAA GCCCAATGAG 
TTCAAGAAGC CCATGCTGTT CGACCGGCAC CTGTGCGTGC GCCAGCTGCG GTACTCAGGA 
ATGATGGAGA CCATCCGAAT CCGCCGAGCT GGCTACCCCA TCCGCTACAG CTTCGTAGAG 
TTTGTGGAGC GGTACCGTGT GCTGCTGCCA GGTGTGAAGC CGGCCTACAA GCAGGGCGAC 
CTCCGCGGGA CTTGCCAGCG CATGGCTGAG GCTGTGCTGG GCACCCACGA TGACTGGCAG 
ATAGGCAAAA CCAAGATCTT TCTGAAGGAC CACCATGACA TGCTGCTGGA AGTGGAGCGG 
GACAAAGCCA TCACCGACAG AGTCATCCTC CTTCAGAAAG TCATCCGGGG ATTCAAAGAC 
AGGTCTAACT TTCTGAAGCT GAAGAACGCT GCCACACTGA TCCAGAGGCA CTGGCGGGGT 
CACAACTGTA GGAAGAACTA CGGGCTGATG CGTCTGGGCT TCCTGCGGCT GCAGGCCCTG 
CACCGCTCCC GGAAGCTGCA CCAGCAGTAC CGCCTGGCCC GCCAGCGCAT CATCCAGTTC 
CAGGCCCGCT GCCGCGCCTA TCTGGTGCGC AAGGCCTTCC GCCACCGCCT CTGGGCTGTG 
CTCACCGTGC AGGCCTATGC CCGGGGCATG ATCGCCCGCA GGCTGCACCA ACGCCTCAGG 
GCTGAGTATC TGTGGCGCCT CGAGGCTGAG AAAATGCGGC TGGCGGAGGA AGAGAAGCTT 
CGGAAGGAGA TGAGCGCCAA GAAGGCCAAG GAGGAGGCCG AGCGCAAGCA TCAGGAGCGC 
CTGGCCCAGC TGGCTCGTGA GGACGCTGAG CGGGAGCTGA AGGAGAAGGA GGCCGCTCGG 
CGGAAGAAGG AGCTCCTGGA GCAGATGGAA AGGGCCCGCC ATGAGCCTGT CAATCACTCA 
GACATGGTGG ACAAGATGTT TGGCTTCCTG GGGACTTCAG GTGGCCTGCC AGGCCAGGAG 
GGCCAGGCAC CTAGTGGCTT TGAGGACCTG GAGCGAGGGC GGAGGGAGAT GGTGGAGGAG 
GACCTGGATG CAGCCCTGCC CCTGCCTGAC GAGGATGAGG AGGACCTCTC TGAGTATAAA 
TTTGCCAAGT TCGCGGCCAC CTACTTCCAG GGGACAACCA CGCACTCCTA CACCCGGCGG 
CCACTCAAAC AGCCACTGCT CTACCATGAC GACGAGGGTG ACCAGCTGGC AGCCCTGGCG 
GTCTGGATCA CCATCCTCCG CTTCATGGGG GACCTCCCTG AGCCCAAGTA CCACACAGCC 
ATGAGTGATG GCAGTGAGAA GATCCCTGTG ATGACCAAGA TTTATGAGAC CCTGGGCAAG 
AAGACGTACA AGAGGGAGCT GCAGGCCCTG CAGGGCGAGG GCGAGGCCCA GCTCCCCGAG 
GGCCAGAAGA AGAGCAGTGT GAGGCACAAG CTGGTGCATT TGACTCTGAA AAAGAAGTCC 
AAGCTCACAG AGGAGGTGAC CAAGAGGCTG CATGACGGGG AGTCCACAGT GCAGGGCAAC 
AGCATGCTGG AGGACCGGCC CACCTCCAAC CTGGAGAAGC TGCACTTCAT CATCGGCAAT 
GGCATCCTGC GGCCAGCACT CCGGGACGAG ATCTACTGCC AGATCAGCAA GCAGCTGACC 
CACAACCCCT CCAAGAGCAG CTATGCCCGG GGCTGGATTC TCGTGTCTCT CTGCGTGGGC 
TGTTTCGCCC CCTCCGAGAA GTTTGTCAAG TACCTGCGGA ACTTCATCCA CGGGGGCCCG 
CCCGGCTACG CCCCGTACTG TGAGGAGCGC CTGAGAAGGA CCTTTGTCAA TGGGACACGG 
ACACAGCCGC CCAGCTGGCT GGAGCTGCAG GCCACCAAGT CCAAGAAGCC AATCATGTTG 
CCCGTGACAT TCATGGATGG GACCACCAAG ACCCTGCTGA CGGACTCGGC AACCACGGCC 
AAGGAGCTCT GCAACGCGCT GGCCGACAAG ATCTCTCTCA AGGACCGGTT CGGGTTCTCC 
CTCTACATTG CCCTGTTTGA CAAGGTGTCC TCCCTGGGCA GCGGCAGTGA CCACGTCATG 
GACGCCATCT CCCAGTGCGA GCAGTACGCC AAGGAGCAGG GCGCCCAGGA GCGCAACGCC 
CCCTGGAGGC TCTTCTTCCG CAAAGAGGTC TTCACGCCCT GGCACAGCCC CTCCGAGGAC 
AACGTGGCCA CCAACCTCAT CTACCAGCAG GTGGTGCGAG GAGTCAAGTT TGGGGAGTAC 
AGGTGTGAGA AGGAGGACGA CCTGGCTGAG CTGGCCTCCC AGCAGTACTT TGTAGACTAT 
GGCTCTGAGA TGATCCTGGA GCGCCTCCTG AACCTCGTGC CCACCTACAT CCCCGACCGC 
GAGATCACGC CCCTGAAGAC GCTGGAGAAG TGGGCCCAGC TGGCCATCGC CGCCCACAAG 
AAGGGGATTT ATGCCCAGAG GAGAACTGAT GCCCAGAAGG TCAAAGAGGA TGTGGTCAGT 
TATGCCCGCT TCAAGTGGCC CTTGCTCTTC TCCAGGTTTT ATGAAGCCTA CAAATTCTCA 
GGCCCCAGTC TCCCCAAGAA CGACGTCATC GTGGCCGTCA ACTGGACGGG TGTGTACTTT 
GTGGATGAGC AGGAGCAGGT ACTTCTGGAG CTGTCCTTCC CAGAGATCAT GGCCGTGTCC 
AGCAGCAGGG AGTGCCGTGT CTGGCTCTCA CTGGGCTGCT CTGATCTTGG CTGTGCTGCG 
CCTCACTCAG GCTGGGCAGG ACTGACCCCG GCGGGGCCCT GTTCTCCGTG TTGGTCCTGC 
AGGGGAGCGA AAACGACGGC CCCCAGCTTC ACGCTGGCCA CCATCAAGGG GGACGAATAC 
ACCTTCACCT CCAGCAATGC TGAGGACATT CGTGACCTGG TGGTCACCTT CCTAGAGGGG 
CTCCGGAAGA GATCTAAGTA TGTTGTGGCC CTGCAGGATA ACCCCAACCC CGCAGGCGAG 
GAGTCAGGCT TCCTCAGCTT TGCCAAGGGA GACCTCATCA TCCTGGACCA TGACACGGGC 
GAGCAGGTCA TGAACTCGGG CTGGGCCAAC GGCATCAATG AGAGGACCAA GCAGCGTGGG 
GACTTCCCCA CCGACAGTGT GTACGTCATG CCCACTGTCA CCATGCCACC GCGGGAGATT 
GTGGCCCTGG TCACCATGAC TCCCGATCAG AGGCAGGACG TTGTCCGGCT CTTGCAGCTG 
CGAACGGCGG AGCCCGAGGT GCGTGCCAAG CCCTACACGC TGGAGGAGTT TTCCTATGAC 
TACTTCAGGC CCCCACCCAA GCACACGCTG AGCCGTGTCA TGGTGTCCAA GGCCCGAGGC 
AAGGACCGGC TGTGGAGCCA CACGCGGGAA CCGCTCAAGC AGGCGCTGCT CAAGAAGCTC 
CTGGGCAGTG AGGAGCTCTC GCAGGAGGCC TGCCTGGCCT TCATTGCTGT GCTCAAGTAC 
ATGGGCGACT ACCCGTCCAA GAGGACACGC TCCGTCAACG AGCTCACCGA CCAGATCTTT 
GAGGGTCCCC TGAAAGCCGA GCCCCTGAAG GACGAGGCAT ATGTGCAGAT CCTGAAGCAG 
CTGACCGACA ACCACATCAG GTACAGCGAG GAGCGGGGTT GGGAGCTGCT CTGGCTGTGC 
ACGGGCCTTT TCCCACCCAG CAACATCCTC CTGCCCCACG TGCAGCGCTT CCTGCAGTCC 
CGAAAGCACT GCCCACTCGC CATCGACTGC CTGCAACGGC TCCAGAAAGC CCTGAGAAAC 
GGGTCCCGGA AGTACCCTCC GCACCTGGTG GAGGTGGAGG CCATCCAGCA CAAGACCACC 
CAGATTTTCC ACAAAGTCTA CTTCCCTGAT GACACTGACG AGGCCTTCGA AGTGGAGTCC 
AGCACCAAGG CCAAGGACTT CTGCCAGAAC ATCGCCACCA GGCTGCTCCT CAAGTCCTCA 
GAGGGATTCA GCCTCTTTGT CAAAATTGCA GACAAGGTCC TCAGCGTTCC TGAGAATGAC 
TTCTTCTTTG ACTTTGTTCG ACACTTGACA GACTGGATAA AGAAAGCTCG GCCCATCAAG 
GACGGAATTG TGCCCTCACT CACCTACCAG GTGTTCTTCA TGAAGAAGCT GTGGACCACC 
ACGGTGCCAG GGAAGGATCC CATGGCCGAT TCCATCTTCC ACTATTACCA GGAGTTGCCC 
AAGTATCTCC GAGGCTACCA CAAGTGCACG CGGGAGGAGG TGCTGCAGCT GGGGGCGCTG 
ATCTACAGGG TCAAGTTCGA GGAGGACAAG TCCTACTTCC CCAGCATCCC CAAGCTGCTG 
CGGGAGCTGG TGCCCCAGGA CCTTATCCGG CAGGTCTCAC CTGATGACTG GAAGCGGTCC 
ATCGTCGCCT ACTTCAACAA GCACGCAGGG AAGTCCAAGG AGGAGGCCAA GCTGGCCTTC 
CTGAAGCTCA TCTTCAAGTG GCCCACCTTT GGCTCAGCCT TCTTCGAGGT GAAGCAAACT 
ACGGAGCCAA ACTTCCCTGA GATCCTCCTA ATTGCCATCA ACAAGTATGG GGTCAGCCTC 
ATCGATCCCA AAACGAAGGA TATCCTCACC ACTCATCCCT TCACCAAGAT CTCCAACTGG 
AGCAGCGGCA ACACCTACTT CCACATCACC ATTGGGAACT TGGTGCGCGG GAGCAAACTG 
CTCTGCGAGA CGTCACTGGG CTACAAGATG GATGACCTCC TGACTTCCTA CATTAGCCAG 
ATGCTCACAG CCATGAGCAA ACAGCGGGGC TCCAGGAGCG GCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MYO7A
Entrez Gene ID	4647
Full Name	myosin VIIA
Synonyms	DFNA11,DFNB2,MYOVIIA,MYU7A,NSRD2,USH1B
General protein information	Preferred Names myosin VIIA Names unconventional myosin-VIIa myosin VIIA (Usher syndrome 1B (autosomal recessive, severe))
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q13.5
Summary	This gene is a member of the myosin gene family. Myosins are mechanochemical proteins characterized by the presence of a motor domain, an actin-binding domain, a neck domain that interacts with other proteins, and a tail domain that serves as an anchor. This gene encodes an unconventional myosin with a very short tail. Defects in this gene are associated with the mouse shaker-1 phenotype and the human Usher syndrome 1B which are characterized by deafness, reduced vestibular function, and (in human) retinal degeneration. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008].
Disorder MIM:	276903
Disorder Html:	Usher syndrome, type 1B, 276900 (3); Deafness, autosomal recessive 2, 600060 (3); Deafness, autosomal dominant 11, 601317 (3)

	MYO7A	XP_001087868.2
Gallus gallus (chicken)	MYO7A	XP_004938998.1
Danio rerio (zebrafish)	myo7aa	NP_694515.1
Rattus norvegicus (Norway rat)	Myo7a	NP_703203.1
Xenopus tropicalis (tropical clawed frog)	myo7a	XP_004912319.1
Homo sapiens (human)	MYO7A	NP_000251.3
Bos taurus (cattle)	MYO7A	XP_005216396.1
Pan troglodytes (chimpanzee)	MYO7A	XP_003313297.2
Canis lupus familiaris (dog)	MYO7A	XP_005633514.1
Mus musculus (house mouse)	Myo7a	NP_001243010.1
Drosophila melanogaster (fruit fly)	ck	NP_523571.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MYO7A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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