kek1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	kek1
Entrez Gene ID	34688
Full Name	kekkon-1
Synonyms	BEST:GM02380,CG12283,CT18186,Dmel\CG12283,Dmkek1,GM02380,KEK1,Kek,Kek-1,Kek1,NB1,SD01674,kek,kek-1,kekon,l(2)01433,n(2)k07332,uns
General protein information	Preferred Names kekkon-1 Names CG12283 gene product from transcript CG12283-RB CG12283-PB CG12283-PC Kekkon-1 Kekkon1 Kekon-1 kek1-PB kek1-PC kekkon kekkon 1 kekkonI unstrung
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 34A1-34A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_078835.3	NP_523559.3	kekkon 1, isoform B
NM_001316391.1	NP_001303320.1	kekkon 1, isoform C

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	kek1	NP_523559.3
Bos taurus (cattle)	LRFN5	NP_001075079.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009136	XP_319897.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

kek1 can suppress bract formation and can interfere with other steps of sensory development, including Sensory Mother Cell determination and shaft differentiation.
Title: Control of bract formation in Drosophila: poxn, kek1, and the EGF-R pathway.

the leucine-rich repeats of Kek1 in conjunction with its juxta/transmembrane region direct association and inhibition of the Drosophila EGFR through interactions with receptor domain V
Title: Bipartite inhibition of Drosophila epidermal growth factor receptor by the extracellular and transmembrane domains of Kekkon1.

Kek1 inhibits EGFR activity during eye development
Title: Knockouts of Kekkon1 define sequence elements essential for Drosophila epidermal growth factor receptor inhibition.

The following kek1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kek1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14584	NM_078835.3 Latest version!	Drosophila melanogaster kekkon 1 (kek1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa14584	NM_001316391.1 Latest version!	Drosophila melanogaster kekkon 1 (kek1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kek1 ( NM_078835.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523559.3 Drosophila melanogaster kekkon 1 (kek1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa14584
Clone ID Related Accession (Same CDS sequence)	NM_078835.3 , NM_001316391.1
Accession Version	NM_078835.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kekkon 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_078835.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATATCA GGGAAGCAGT TTTCCTGGTC CTCACCCTGC TGCCTGGAAT GATCCTGGGC 
ACTCGCTACA ATCAGCTGCA TCTGTATGCC AATGGAGGAG CATCGTCATC GGGCCCTGGA 
GGCTACAGGC CCGCCCCCTC GTCCCAGAAC GAGGTGTACT CCATAGCGGA CAGCCAGCCG 
ATGACTGAGG ATGGCTACAT GCCCCCCAGC CAGCACTTTC CGCCCACCCA CTCCGACTTG 
GATCCCCCCG CCCAGCAGCA GAGCACCTGC CAAACGGTTT GCGCCTGCAA GTGGAAGGGT 
GGCAAGCAGA CGGTGGAGTG CATCGATCGC CACCTCATCC AGATACCCGA GCACATCGAT 
CCCAATACCC AGGTGCTGGA CATGTCCGGT AATAAGCTGC AGACCCTCTC CAACGAGCAG 
TTCATCCGTG CGAATCTGCT AAATCTGCAG AAGCTGTATT TGAGGAACTG CAAGATCGGC 
GAAATCGAGC GGGAGACCTT CAAGGGACTG ACCAATCTGG TGGAGTTGGA TCTGTCACAT 
AATCTGCTGG TTACCGTGCC CAGTTTGGCC CTGGGCCACA TACCCTCACT GCGCGAACTC 
ACCCTGGCCT CCAATCACAT ACACAAAATC GAGAGCCAGG CCTTCGGGAA CACACCATCG 
CTGCACAAAT TGGATCTGTC GCATTGCGAT ATTCAGACCA TTTCCGCCCA GGCATTTGGT 
GGCCTCCAAG GATTGACGTT GCTCCGATTG AATGGCAATA AACTGAGCGA GCTTTTGCCC 
AAGACAATTG AGACCCTGAG TCGTCTTCAT GGCATCGAAC TGCACGACAA TCCCTGGCTC 
TGTGATTGTC GATTGAGGGA CACGAAGCTC TGGCTGATGA AGAGGAACAT ACCCTATCCG 
GTGGCACCGG TTTGCTCGGG TGGCCCCGAA AGGATTATCG ATCGCAGCTT TGCGGATCTG 
CATGTGGATG AGTTTGCCTG CCGACCGGAG ATGTTGCCCA TATCGCATTA TGTGGAGGCG 
GCCATGGGCG AGAATGCCTC GATTACATGT CGAGCTCGAG CGGTTCCAGC TGCGAATATC 
AACTGGTACT GGAACGGACG GCTGCTGGCC AACAATTCCG CCTTCACCGC GTACCAGAGG 
ATACACATGT TGGAGCAGGT GGAAGGTGGA TTCGAAAAGC GATCCAAACT GGTGCTGACC 
AACGCACAGG AAACGGATTC CAGTGAGTTC TACTGCGTGG CCGAGAATCG AGCTGGGATG 
GCCGAGGCCA ACTTCACCCT GCACGTGAGC ATGAGAGCTG CGGGCATGGC CTCCCTGGGT 
AGTGGCCAAA TTGTGGGTCT GAGTGCCGCC CTGGTTGCTC TGATTGTGTT TGCCCTTGGG 
GTTATCATGT GCCTGCTCCT GAGGGTAAAA CGGCAGCCGT ATGTCGATAG CAAGACGCCC 
AATCACATGG AGGTGATAAC ATCTGTTAAC CACCAGAACT CCATAACAAA CAAGACGCAG 
CCCGCAACGG GAAATGGCAG TATTGGCGGC GTGGTCATCG CCAATGGAGC TGTGGCCAAC 
ATAATCGATG GCGGAGTGGT GCAGGGAGGA ACTCTGGAGC GGAAAAGCAG CGGACGGGGA 
GGTGTACCGC ATGGAGTTCA CGATCAGCGC AGTGCAAATC CCGTGCAGAA ACCGCCGAGG 
CTAACAGATC TTCCGTACTC TACGCAGGGC TATGACAACA ACGGAAGTGT CCTGTCCACT 
GCCTCCTGTT TCATCTCGCC CAGTGGATCC ACCGGAAACG GTGGCAACAA TCCTGATCTC 
ATTAATGATA CCAAACGTTT TGGGAGCGAC GAGTTTGCGG ATCTGAAGAT ACCACCCATC 
AGTGGTGTTG GAGTCGGCGG CAGTGGGGAG TATAGTCGCG CCAACGGCTG CGATTCCCTG 
TATCCTTCGG GTCTGTGGGA ACATGGTGCT CCAGTGGGCA CCACATCCGC GGATGACCTC 
TTCATGAAGC GCTACACCGA CAAGACGCCC ATCATAGACT CCACACAGCT GTACGACCTT 
CATGAGCGAA CGGCGGCCAC GGATTATTTT AGCAAGACAT TCCCGAGATC TCACCTCCAG 
CAGGGCATGA TGACGGGTGG CGGTGGAGGA ACCTCGACGG CGTCGACGGT AACCACTAAT 
TTGTCGGGTG GCTCCTCATC GGGTTACCCC AACGATTATG GTCTGCCTCT GGTGCCGGGG 
GCAGAGCACC AGCACAACCA CCAGCTGCAG ATGCATCCAC TGCAGCAGCT CCAGCAGCAG 
CTGACCTCCA CGCTGAACCA TCAGAAGCAG GAGGGCAGCT CCACCGGGAG CAGTCCGCAC 
TTCAGTAGCC GCACACTGCC ACGCCTGCAC GAGGGCAGTG GCGGGGGCGG CAGTTCACGG 
TCGTCGCCGA CGCCAGCGGT TAGCGGTGGC CATGCCAACC AGGCGGCAAA TCCCAGCACC 
TCCAGTTCCT CCTGCTCCAT CCTGCCCAAC GGGCAGCCAA TTAACGCCAA GACGATACGG 
GTGTGGCAAA AGGGCGGTGT GCCCGTCCTG CCACCCGTGA CGGCGCTGAA AAGGGCCCTG 
ATCAGCAGCA GCCGGAATTC GCCGGACGAG GGATACCAGG AAGGATGCGG CACGGATGTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523559.3
CDS	1128..3770
Translation	MHIREAVFLVLTLLPGMILGTRYNQLHLYANGGASSSGPGGYRPAPSSQNEVYSIADSQPMTEDGYMPPSQHFPPTHSDLDPPAQQQSTCQTVCACKWKGGKQTVECIDRHLIQIPEHIDPNTQVLDMSGNKLQTLSNEQFIRANLLNLQKLYLRNCKIGEIERETFKGLTNLVELDLSHNLLVTVPSLALGHIPSLRELTLASNHIHKIESQAFGNTPSLHKLDLSHCDIQTISAQAFGGLQGLTLLRLNGNKLSELLPKTIETLSRLHGIELHDNPWLCDCRLRDTKLWLMKRNIPYPVAPVCSGGPERIIDRSFADLHVDEFACRPEMLPISHYVEAAMGENASITCRARAVPAANINWYWNGRLLANNSAFTAYQRIHMLEQVEGGFEKRSKLVLTNAQETDSSEFYCVAENRAGMAEANFTLHVSMRAAGMASLGSGQIVGLSAALVALIVFALGVIMCLLLRVKRQPYVDSKTPNHMEVITSVNHQNSITNKTQPATGNGSIGGVVIANGAVANIIDGGVVQGGTLERKSSGRGGVPHGVHDQRSANPVQKPPRLTDLPYSTQGYDNNGSVLSTASCFISPSGSTGNGGNNPDLINDTKRFGSDEFADLKIPPISGVGVGGSGEYSRANGCDSLYPSGLWEHGAPVGTTSADDLFMKRYTDKTPIIDSTQLYDLHERTAATDYFSKTFPRSHLQQGMMTGGGGGTSTASTVTTNLSGGSSSGYPNDYGLPLVPGAEHQHNHQLQMHPLQQLQQQLTSTLNHQKQEGSSTGSSPHFSSRTLPRLHEGSGGGGSSRSSPTPAVSGGHANQAANPSTSSSSCSILPNGQPINAKTIRVWQKGGVPVLPPVTALKRALISSSRNSPDEGYQEGCGTDV

Target ORF information:

RefSeq Version	NM_078835.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kekkon 1 (kek1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078835.3

ORF Insert Sequence:

ATGCATATCA GGGAAGCAGT TTTCCTGGTC CTCACCCTGC TGCCTGGAAT GATCCTGGGC 
ACTCGCTACA ATCAGCTGCA TCTGTATGCC AATGGAGGAG CATCGTCATC GGGCCCTGGA 
GGCTACAGGC CCGCCCCCTC GTCCCAGAAC GAGGTGTACT CCATAGCGGA CAGCCAGCCG 
ATGACTGAGG ATGGCTACAT GCCCCCCAGC CAGCACTTTC CGCCCACCCA CTCCGACTTG 
GATCCCCCCG CCCAGCAGCA GAGCACCTGC CAAACGGTTT GCGCCTGCAA GTGGAAGGGT 
GGCAAGCAGA CGGTGGAGTG CATCGATCGC CACCTCATCC AGATACCCGA GCACATCGAT 
CCCAATACCC AGGTGCTGGA CATGTCCGGT AATAAGCTGC AGACCCTCTC CAACGAGCAG 
TTCATCCGTG CGAATCTGCT AAATCTGCAG AAGCTGTATT TGAGGAACTG CAAGATCGGC 
GAAATCGAGC GGGAGACCTT CAAGGGACTG ACCAATCTGG TGGAGTTGGA TCTGTCACAT 
AATCTGCTGG TTACCGTGCC CAGTTTGGCC CTGGGCCACA TACCCTCACT GCGCGAACTC 
ACCCTGGCCT CCAATCACAT ACACAAAATC GAGAGCCAGG CCTTCGGGAA CACACCATCG 
CTGCACAAAT TGGATCTGTC GCATTGCGAT ATTCAGACCA TTTCCGCCCA GGCATTTGGT 
GGCCTCCAAG GATTGACGTT GCTCCGATTG AATGGCAATA AACTGAGCGA GCTTTTGCCC 
AAGACAATTG AGACCCTGAG TCGTCTTCAT GGCATCGAAC TGCACGACAA TCCCTGGCTC 
TGTGATTGTC GATTGAGGGA CACGAAGCTC TGGCTGATGA AGAGGAACAT ACCCTATCCG 
GTGGCACCGG TTTGCTCGGG TGGCCCCGAA AGGATTATCG ATCGCAGCTT TGCGGATCTG 
CATGTGGATG AGTTTGCCTG CCGACCGGAG ATGTTGCCCA TATCGCATTA TGTGGAGGCG 
GCCATGGGCG AGAATGCCTC GATTACATGT CGAGCTCGAG CGGTTCCAGC TGCGAATATC 
AACTGGTACT GGAACGGACG GCTGCTGGCC AACAATTCCG CCTTCACCGC GTACCAGAGG 
ATACACATGT TGGAGCAGGT GGAAGGTGGA TTCGAAAAGC GATCCAAACT GGTGCTGACC 
AACGCACAGG AAACGGATTC CAGTGAGTTC TACTGCGTGG CCGAGAATCG AGCTGGGATG 
GCCGAGGCCA ACTTCACCCT GCACGTGAGC ATGAGAGCTG CGGGCATGGC CTCCCTGGGT 
AGTGGCCAAA TTGTGGGTCT GAGTGCCGCC CTGGTTGCTC TGATTGTGTT TGCCCTTGGG 
GTTATCATGT GCCTGCTCCT GAGGGTAAAA CGGCAGCCGT ATGTCGATAG CAAGACGCCC 
AATCACATGG AGGTGATAAC ATCTGTTAAC CACCAGAACT CCATAACAAA CAAGACGCAG 
CCCGCAACGG GAAATGGCAG TATTGGCGGC GTGGTCATCG CCAATGGAGC TGTGGCCAAC 
ATAATCGATG GCGGAGTGGT GCAGGGAGGA ACTCTGGAGC GGAAAAGCAG CGGACGGGGA 
GGTGTACCGC ATGGAGTTCA CGATCAGCGC AGTGCAAATC CCGTGCAGAA ACCGCCGAGG 
CTAACAGATC TTCCGTACTC TACGCAGGGC TATGACAACA ACGGAAGTGT CCTGTCCACT 
GCCTCCTGTT TCATCTCGCC CAGTGGATCC ACCGGAAACG GTGGCAACAA TCCTGATCTC 
ATTAATGATA CCAAACGTTT TGGGAGCGAC GAGTTTGCGG ATCTGAAGAT ACCACCCATC 
AGTGGTGTTG GAGTCGGCGG CAGTGGGGAG TATAGTCGCG CCAACGGCTG CGATTCCCTG 
TATCCTTCGG GTCTGTGGGA ACATGGTGCT CCAGTGGGCA CCACATCCGC GGATGACCTC 
TTCATGAAGC GCTACACCGA CAAGACGCCC ATCATAGACT CCACACAGCT GTACGACCTT 
CATGAGCGAA CGGCGGCCAC GGATTATTTT AGCAAGACAT TCCCGAGATC TCACCTCCAG 
CAGGGCATGA TGACGGGTGG CGGTGGAGGA ACCTCGACGG CGTCGACGGT AACCACTAAT 
TTGTCGGGTG GCTCCTCATC GGGTTACCCC AACGATTATG GTCTGCCTCT GGTGCCGGGG 
GCAGAGCACC AGCACAACCA CCAGCTGCAG ATGCATCCAC TGCAGCAGCT CCAGCAGCAG 
CTGACCTCCA CGCTGAACCA TCAGAAGCAG GAGGGCAGCT CCACCGGGAG CAGTCCGCAC 
TTCAGTAGCC GCACACTGCC ACGCCTGCAC GAGGGCAGTG GCGGGGGCGG CAGTTCACGG 
TCGTCGCCGA CGCCAGCGGT TAGCGGTGGC CATGCCAACC AGGCGGCAAA TCCCAGCACC 
TCCAGTTCCT CCTGCTCCAT CCTGCCCAAC GGGCAGCCAA TTAACGCCAA GACGATACGG 
GTGTGGCAAA AGGGCGGTGT GCCCGTCCTG CCACCCGTGA CGGCGCTGAA AAGGGCCCTG 
ATCAGCAGCA GCCGGAATTC GCCGGACGAG GGATACCAGG AAGGATGCGG CACGGATGTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kek1 ( NM_001316391.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001303320.1 Drosophila melanogaster kekkon 1 (kek1), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa14584
Clone ID Related Accession (Same CDS sequence)	NM_078835.3 , NM_001316391.1
Accession Version	NM_001316391.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	kekkon 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATATCA GGGAAGCAGT TTTCCTGGTC CTCACCCTGC TGCCTGGAAT GATCCTGGGC 
ACTCGCTACA ATCAGCTGCA TCTGTATGCC AATGGAGGAG CATCGTCATC GGGCCCTGGA 
GGCTACAGGC CCGCCCCCTC GTCCCAGAAC GAGGTGTACT CCATAGCGGA CAGCCAGCCG 
ATGACTGAGG ATGGCTACAT GCCCCCCAGC CAGCACTTTC CGCCCACCCA CTCCGACTTG 
GATCCCCCCG CCCAGCAGCA GAGCACCTGC CAAACGGTTT GCGCCTGCAA GTGGAAGGGT 
GGCAAGCAGA CGGTGGAGTG CATCGATCGC CACCTCATCC AGATACCCGA GCACATCGAT 
CCCAATACCC AGGTGCTGGA CATGTCCGGT AATAAGCTGC AGACCCTCTC CAACGAGCAG 
TTCATCCGTG CGAATCTGCT AAATCTGCAG AAGCTGTATT TGAGGAACTG CAAGATCGGC 
GAAATCGAGC GGGAGACCTT CAAGGGACTG ACCAATCTGG TGGAGTTGGA TCTGTCACAT 
AATCTGCTGG TTACCGTGCC CAGTTTGGCC CTGGGCCACA TACCCTCACT GCGCGAACTC 
ACCCTGGCCT CCAATCACAT ACACAAAATC GAGAGCCAGG CCTTCGGGAA CACACCATCG 
CTGCACAAAT TGGATCTGTC GCATTGCGAT ATTCAGACCA TTTCCGCCCA GGCATTTGGT 
GGCCTCCAAG GATTGACGTT GCTCCGATTG AATGGCAATA AACTGAGCGA GCTTTTGCCC 
AAGACAATTG AGACCCTGAG TCGTCTTCAT GGCATCGAAC TGCACGACAA TCCCTGGCTC 
TGTGATTGTC GATTGAGGGA CACGAAGCTC TGGCTGATGA AGAGGAACAT ACCCTATCCG 
GTGGCACCGG TTTGCTCGGG TGGCCCCGAA AGGATTATCG ATCGCAGCTT TGCGGATCTG 
CATGTGGATG AGTTTGCCTG CCGACCGGAG ATGTTGCCCA TATCGCATTA TGTGGAGGCG 
GCCATGGGCG AGAATGCCTC GATTACATGT CGAGCTCGAG CGGTTCCAGC TGCGAATATC 
AACTGGTACT GGAACGGACG GCTGCTGGCC AACAATTCCG CCTTCACCGC GTACCAGAGG 
ATACACATGT TGGAGCAGGT GGAAGGTGGA TTCGAAAAGC GATCCAAACT GGTGCTGACC 
AACGCACAGG AAACGGATTC CAGTGAGTTC TACTGCGTGG CCGAGAATCG AGCTGGGATG 
GCCGAGGCCA ACTTCACCCT GCACGTGAGC ATGAGAGCTG CGGGCATGGC CTCCCTGGGT 
AGTGGCCAAA TTGTGGGTCT GAGTGCCGCC CTGGTTGCTC TGATTGTGTT TGCCCTTGGG 
GTTATCATGT GCCTGCTCCT GAGGGTAAAA CGGCAGCCGT ATGTCGATAG CAAGACGCCC 
AATCACATGG AGGTGATAAC ATCTGTTAAC CACCAGAACT CCATAACAAA CAAGACGCAG 
CCCGCAACGG GAAATGGCAG TATTGGCGGC GTGGTCATCG CCAATGGAGC TGTGGCCAAC 
ATAATCGATG GCGGAGTGGT GCAGGGAGGA ACTCTGGAGC GGAAAAGCAG CGGACGGGGA 
GGTGTACCGC ATGGAGTTCA CGATCAGCGC AGTGCAAATC CCGTGCAGAA ACCGCCGAGG 
CTAACAGATC TTCCGTACTC TACGCAGGGC TATGACAACA ACGGAAGTGT CCTGTCCACT 
GCCTCCTGTT TCATCTCGCC CAGTGGATCC ACCGGAAACG GTGGCAACAA TCCTGATCTC 
ATTAATGATA CCAAACGTTT TGGGAGCGAC GAGTTTGCGG ATCTGAAGAT ACCACCCATC 
AGTGGTGTTG GAGTCGGCGG CAGTGGGGAG TATAGTCGCG CCAACGGCTG CGATTCCCTG 
TATCCTTCGG GTCTGTGGGA ACATGGTGCT CCAGTGGGCA CCACATCCGC GGATGACCTC 
TTCATGAAGC GCTACACCGA CAAGACGCCC ATCATAGACT CCACACAGCT GTACGACCTT 
CATGAGCGAA CGGCGGCCAC GGATTATTTT AGCAAGACAT TCCCGAGATC TCACCTCCAG 
CAGGGCATGA TGACGGGTGG CGGTGGAGGA ACCTCGACGG CGTCGACGGT AACCACTAAT 
TTGTCGGGTG GCTCCTCATC GGGTTACCCC AACGATTATG GTCTGCCTCT GGTGCCGGGG 
GCAGAGCACC AGCACAACCA CCAGCTGCAG ATGCATCCAC TGCAGCAGCT CCAGCAGCAG 
CTGACCTCCA CGCTGAACCA TCAGAAGCAG GAGGGCAGCT CCACCGGGAG CAGTCCGCAC 
TTCAGTAGCC GCACACTGCC ACGCCTGCAC GAGGGCAGTG GCGGGGGCGG CAGTTCACGG 
TCGTCGCCGA CGCCAGCGGT TAGCGGTGGC CATGCCAACC AGGCGGCAAA TCCCAGCACC 
TCCAGTTCCT CCTGCTCCAT CCTGCCCAAC GGGCAGCCAA TTAACGCCAA GACGATACGG 
GTGTGGCAAA AGGGCGGTGT GCCCGTCCTG CCACCCGTGA CGGCGCTGAA AAGGGCCCTG 
ATCAGCAGCA GCCGGAATTC GCCGGACGAG GGATACCAGG AAGGATGCGG CACGGATGTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303320.1
CDS	1128..3770
Translation	MHIREAVFLVLTLLPGMILGTRYNQLHLYANGGASSSGPGGYRPAPSSQNEVYSIADSQPMTEDGYMPPSQHFPPTHSDLDPPAQQQSTCQTVCACKWKGGKQTVECIDRHLIQIPEHIDPNTQVLDMSGNKLQTLSNEQFIRANLLNLQKLYLRNCKIGEIERETFKGLTNLVELDLSHNLLVTVPSLALGHIPSLRELTLASNHIHKIESQAFGNTPSLHKLDLSHCDIQTISAQAFGGLQGLTLLRLNGNKLSELLPKTIETLSRLHGIELHDNPWLCDCRLRDTKLWLMKRNIPYPVAPVCSGGPERIIDRSFADLHVDEFACRPEMLPISHYVEAAMGENASITCRARAVPAANINWYWNGRLLANNSAFTAYQRIHMLEQVEGGFEKRSKLVLTNAQETDSSEFYCVAENRAGMAEANFTLHVSMRAAGMASLGSGQIVGLSAALVALIVFALGVIMCLLLRVKRQPYVDSKTPNHMEVITSVNHQNSITNKTQPATGNGSIGGVVIANGAVANIIDGGVVQGGTLERKSSGRGGVPHGVHDQRSANPVQKPPRLTDLPYSTQGYDNNGSVLSTASCFISPSGSTGNGGNNPDLINDTKRFGSDEFADLKIPPISGVGVGGSGEYSRANGCDSLYPSGLWEHGAPVGTTSADDLFMKRYTDKTPIIDSTQLYDLHERTAATDYFSKTFPRSHLQQGMMTGGGGGTSTASTVTTNLSGGSSSGYPNDYGLPLVPGAEHQHNHQLQMHPLQQLQQQLTSTLNHQKQEGSSTGSSPHFSSRTLPRLHEGSGGGGSSRSSPTPAVSGGHANQAANPSTSSSSCSILPNGQPINAKTIRVWQKGGVPVLPPVTALKRALISSSRNSPDEGYQEGCGTDV

Target ORF information:

RefSeq Version	NM_001316391.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster kekkon 1 (kek1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316391.1

ORF Insert Sequence:

ATGCATATCA GGGAAGCAGT TTTCCTGGTC CTCACCCTGC TGCCTGGAAT GATCCTGGGC 
ACTCGCTACA ATCAGCTGCA TCTGTATGCC AATGGAGGAG CATCGTCATC GGGCCCTGGA 
GGCTACAGGC CCGCCCCCTC GTCCCAGAAC GAGGTGTACT CCATAGCGGA CAGCCAGCCG 
ATGACTGAGG ATGGCTACAT GCCCCCCAGC CAGCACTTTC CGCCCACCCA CTCCGACTTG 
GATCCCCCCG CCCAGCAGCA GAGCACCTGC CAAACGGTTT GCGCCTGCAA GTGGAAGGGT 
GGCAAGCAGA CGGTGGAGTG CATCGATCGC CACCTCATCC AGATACCCGA GCACATCGAT 
CCCAATACCC AGGTGCTGGA CATGTCCGGT AATAAGCTGC AGACCCTCTC CAACGAGCAG 
TTCATCCGTG CGAATCTGCT AAATCTGCAG AAGCTGTATT TGAGGAACTG CAAGATCGGC 
GAAATCGAGC GGGAGACCTT CAAGGGACTG ACCAATCTGG TGGAGTTGGA TCTGTCACAT 
AATCTGCTGG TTACCGTGCC CAGTTTGGCC CTGGGCCACA TACCCTCACT GCGCGAACTC 
ACCCTGGCCT CCAATCACAT ACACAAAATC GAGAGCCAGG CCTTCGGGAA CACACCATCG 
CTGCACAAAT TGGATCTGTC GCATTGCGAT ATTCAGACCA TTTCCGCCCA GGCATTTGGT 
GGCCTCCAAG GATTGACGTT GCTCCGATTG AATGGCAATA AACTGAGCGA GCTTTTGCCC 
AAGACAATTG AGACCCTGAG TCGTCTTCAT GGCATCGAAC TGCACGACAA TCCCTGGCTC 
TGTGATTGTC GATTGAGGGA CACGAAGCTC TGGCTGATGA AGAGGAACAT ACCCTATCCG 
GTGGCACCGG TTTGCTCGGG TGGCCCCGAA AGGATTATCG ATCGCAGCTT TGCGGATCTG 
CATGTGGATG AGTTTGCCTG CCGACCGGAG ATGTTGCCCA TATCGCATTA TGTGGAGGCG 
GCCATGGGCG AGAATGCCTC GATTACATGT CGAGCTCGAG CGGTTCCAGC TGCGAATATC 
AACTGGTACT GGAACGGACG GCTGCTGGCC AACAATTCCG CCTTCACCGC GTACCAGAGG 
ATACACATGT TGGAGCAGGT GGAAGGTGGA TTCGAAAAGC GATCCAAACT GGTGCTGACC 
AACGCACAGG AAACGGATTC CAGTGAGTTC TACTGCGTGG CCGAGAATCG AGCTGGGATG 
GCCGAGGCCA ACTTCACCCT GCACGTGAGC ATGAGAGCTG CGGGCATGGC CTCCCTGGGT 
AGTGGCCAAA TTGTGGGTCT GAGTGCCGCC CTGGTTGCTC TGATTGTGTT TGCCCTTGGG 
GTTATCATGT GCCTGCTCCT GAGGGTAAAA CGGCAGCCGT ATGTCGATAG CAAGACGCCC 
AATCACATGG AGGTGATAAC ATCTGTTAAC CACCAGAACT CCATAACAAA CAAGACGCAG 
CCCGCAACGG GAAATGGCAG TATTGGCGGC GTGGTCATCG CCAATGGAGC TGTGGCCAAC 
ATAATCGATG GCGGAGTGGT GCAGGGAGGA ACTCTGGAGC GGAAAAGCAG CGGACGGGGA 
GGTGTACCGC ATGGAGTTCA CGATCAGCGC AGTGCAAATC CCGTGCAGAA ACCGCCGAGG 
CTAACAGATC TTCCGTACTC TACGCAGGGC TATGACAACA ACGGAAGTGT CCTGTCCACT 
GCCTCCTGTT TCATCTCGCC CAGTGGATCC ACCGGAAACG GTGGCAACAA TCCTGATCTC 
ATTAATGATA CCAAACGTTT TGGGAGCGAC GAGTTTGCGG ATCTGAAGAT ACCACCCATC 
AGTGGTGTTG GAGTCGGCGG CAGTGGGGAG TATAGTCGCG CCAACGGCTG CGATTCCCTG 
TATCCTTCGG GTCTGTGGGA ACATGGTGCT CCAGTGGGCA CCACATCCGC GGATGACCTC 
TTCATGAAGC GCTACACCGA CAAGACGCCC ATCATAGACT CCACACAGCT GTACGACCTT 
CATGAGCGAA CGGCGGCCAC GGATTATTTT AGCAAGACAT TCCCGAGATC TCACCTCCAG 
CAGGGCATGA TGACGGGTGG CGGTGGAGGA ACCTCGACGG CGTCGACGGT AACCACTAAT 
TTGTCGGGTG GCTCCTCATC GGGTTACCCC AACGATTATG GTCTGCCTCT GGTGCCGGGG 
GCAGAGCACC AGCACAACCA CCAGCTGCAG ATGCATCCAC TGCAGCAGCT CCAGCAGCAG 
CTGACCTCCA CGCTGAACCA TCAGAAGCAG GAGGGCAGCT CCACCGGGAG CAGTCCGCAC 
TTCAGTAGCC GCACACTGCC ACGCCTGCAC GAGGGCAGTG GCGGGGGCGG CAGTTCACGG 
TCGTCGCCGA CGCCAGCGGT TAGCGGTGGC CATGCCAACC AGGCGGCAAA TCCCAGCACC 
TCCAGTTCCT CCTGCTCCAT CCTGCCCAAC GGGCAGCCAA TTAACGCCAA GACGATACGG 
GTGTGGCAAA AGGGCGGTGT GCCCGTCCTG CCACCCGTGA CGGCGCTGAA AAGGGCCCTG 
ATCAGCAGCA GCCGGAATTC GCCGGACGAG GGATACCAGG AAGGATGCGG CACGGATGTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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kek1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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