Myo31DF cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Myo31DF
Entrez Gene ID	34445
Full Name	Myosin 31DF
Synonyms	CG7438,Dm IA,DmIA,Dmel\CG7438,DroMIA,IA,MYOIA,Mhc1,Myo-IA,Myo1A,Myo1D,MyoIA,MyoID,myo31DF,myoID,souther
General protein information	Preferred Names Myosin 31DF Names CG7438 gene product from transcript CG7438-RA CG7438-PA CG7438-PB CG7438-PC Myo31DF-PA Myo31DF-PB Myo31DF-PC Myosin31DF myosin 1D myosin 1a myosin 31DF myosin I myosin IA myosin ID myosin-IA myosin1A souther type ID unconventional myosin 31DF unconventional myosin 31DF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 31F1-31F4

mRNA and Protein(s)

mRNA	Protein	Name
NM_078814.4	NP_523538.1	myosin 31DF, isoform A
NM_164933.3	NP_723596.1	myosin 31DF, isoform B
NM_001201855.2	NP_001188784.1	myosin 31DF, isoform C

Pathways from BioSystems

Homologies

Homo sapiens (human)	MYO1D	NP_056009.1
Mus musculus (house mouse)	Myo1d	NP_796364.2
Pan troglodytes (chimpanzee)	MYO1D	XP_511404.3
	MYO1D	XP_001110356.2
Rattus norvegicus (Norway rat)	Myo1d	NP_037115.2
Drosophila melanogaster (fruit fly)	Myo31DF	NP_723596.1
Canis lupus familiaris (dog)	MYO1D	XP_548273.3
Bos taurus (cattle)	MYO1D	NP_001069306.1
Gallus gallus (chicken)	MYO1D	XP_418085.3
Danio rerio (zebrafish)	si:ch211-94l19.4	XP_688008.5
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010160	XP_319330.4
Caenorhabditis elegans (roundworm)	hum-5	NP_497809.1
Xenopus tropicalis (tropical clawed frog)	myo1d	XP_002935562.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Myo31DF-overexpressing cells suggest that cell-shape chirality was established in each cell and reflects intrinsic planar cell chirality.
Title: Left-right asymmetry is formed in individual cells by intrinsic cell chirality.

DE-Cadherin regulates unconventional Myosin ID and Myosin IC in Drosophila left-right asymmetry establishment.
Title: DE-Cadherin regulates unconventional Myosin ID and Myosin IC in Drosophila left-right asymmetry establishment.

left-right(LR)directional rotation of hindgut epithelial tube; DECad is distributed to cell boundaries with LR asymmetry which is responsible for planar cell-shape chirality formation; myosin ID switches LR polarity found in PCC and in DE-Cad distribution
Title: Chirality in planar cell shape contributes to left-right asymmetric epithelial morphogenesis.

the organ specificities of the Myo31DF and Myo61F activities depended on their head regions
Title: Head region of unconventional myosin I family members is responsible for the organ-specificity of their roles in left-right polarity in Drosophila.

Myo31DF interacts and colocalizes with beta-catenin, suggesting that situs inversus genes can direct left-right development through the adherens junction
Title: Type ID unconventional myosin controls left-right asymmetry in Drosophila.

The following Myo31DF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Myo31DF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14370	NM_164933.3 Latest version!	Drosophila melanogaster myosin 31DF, transcript variant B (Myo31DF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa14370	NM_001201855.2 Latest version!	Drosophila melanogaster myosin 31DF, transcript variant C (Myo31DF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa14370	NM_078814.4 Latest version!	Drosophila melanogaster myosin 31DF, transcript variant A (Myo31DF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Myo31DF ( NM_078814.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523538.1 Drosophila melanogaster myosin 31DF, transcript variant A (Myo31DF), mRNA.

Price & Availability↑

CloneID	OFa14370
Clone ID Related Accession (Same CDS sequence)	NM_078814.4 , NM_164933.3 , NM_001201855.2
Accession Version	NM_078814.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin 31DF, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_078814.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTATGC AACGGGAAGC TGGAGTGCAG GACTTCGTCC TCCTCGACCA AGTGTCCATG 
GAGAAGTTCA TGGATAACCT GAGAAAGCGA TTCCAAAACG GATCCATCTA CACATACATC 
GGCGAAGTGT GCGTCTCGAT GAATCCCTAC AGACAGATGA ACATCTACGG TCCGGAAACC 
ATTCGCAAAT ACAAGGGTCG GGAGCTCTTC GAGAATGCCC CACATTTGTT CGCCATTGCC 
GATTCCGCCT ACAGGGTGCT CAAGCAGCGC CAACAAGACA CCTGCATCCT AATTTCCGGT 
GAGTCCGGTG CGGGCAAAAC GGAGGCCTCC AAGATCATCA TGAAGTACAT TGCGGCGGTG 
ACGAATGCCC AGGGTCAGAA CGAAATCGAG AGAGTCAAGA ACGTTTTGAT CCAGAGCAAT 
GCAATTCTGG AAACCTTCGG CAACGCAAAG ACTAACCGCA ACGACAACTC CAGCCGCTTC 
GGAAAGTACA TGGACATTGA GTTCGACTAC AAGGCGGATC CCGTGGGTGG CATCATCACC 
AACTATCTGC TCGAGAAGTC GCGCGTGGTG CAGCAGCAGC CCGGCGAGCG GAACTTCCAT 
AGTTTCTATC AGCTGCTGCG TGGAGCCAAC GACAACGAGC TGCGGCAATA TGAACTGCAG 
AAGGAAACGG GCAAGTATCA CTACTTGAAC CAGGGCAGCA TGGACATACT GACGGAGAAG 
TCCGACTACA AGGGCACCTG CAATGCCTTC AAGACCCTGG GCTTCTCCAC GGATGAGGTG 
CAGACCATTT GGCGCACAAT TGCCGCTGTC TTGCATCTGG GAAATGTGGA GTTCCAAACC 
ATTGAGGATG AGCTGGTGAT CAGCAATAAG CAGCACTTGA AATCCACGGC CAAGCTGCTC 
CAGGTCACCG AAACCGAGCT GTCCACGGCA CTGACCAAAC GGGTCATTGC GGCCGGTGGA 
AATGTGATGC AGAAGGATCA CAACGCCACG CAGGCAGAGT ACGGAAAGGA TGCTCTGGCC 
AAGGCCATCT ACGACCGTCT CTTCACCTGG ATCATCTCCC GCATCAACAG GGCAATCCTA 
TTCCGTGGCA GCAAGACGCA GGCGCGATTT AACTCGGTCA TCGGAGTGCT GGATATTTAC 
GGCTTCGAGA TCTTCGACAG CAACAGCTTT GAACAGTTCT GCATCAACTA TTGCAACGAG 
AAATTGCAGC AGTTATTCAT AGAACTGGTG CTTAAACAGG AGCAAGAGGA GTACCAGCGT 
GAGGGCATTG AATGGACCAA TATTGAATAC TTCAACAACA AGATCATTTG CGATCTCGTT 
GAGCAGCCGC ATAAGGGCAT CATTGCCATC ATGGACGAGG CCTGCCTCAG CGTGGGCAAG 
GTGACGGACG ACACACTTCT GGGGGCCATG GACAAAAATC TGAGCAAGCA TCCGCACTAC 
ACCAGTCGTC AGCTGAAGCC CACGGATAAG GAGCTAAAGC ATCGCGAGGA TTTCCGTATC 
ACCCACTACG CCGGCGATGT GATCTACAAC ATCAATGGAT TCATCGAGAA GAACAAGGAT 
ACGCTGTATC AGGACTTTAA GCGCTTGCTG CACAACTCTA AGGATGCCAA CCTGAGCGAG 
ATGTGGCCCG AAGGAGCACA GGATATCAAG AAAACCACCA AGAGACCATT GACAGCCGGC 
ACCTTGTTCC AGAGATCGAT GGCTGATCTG GTGGTTACTC TGCTGAAGAA GGAACCCTTC 
TATGTGCGCT GCATCAAGCC CAATGACCTT AAGAGCTCGA CAGTTTTCGA TGAGGAGCGT 
GTGGAGCACC AGGTGCGATA CCTTGGCCTA CTGGAGAACT TACGTGTCCG TCGAGCCGGA 
TTCGTTCATC GCCAGCGCTA TGATAAGTTC CTGCTGCGCT ACAAGATGAT CTCGCAGTAC 
ACTTGGCCCA ATTTCCGTGC GGGAAGCGAC CGCGATGGTG TCCGTGTGCT GATCGAGGAA 
AAGAAATTCG CCCAGGACGT TAAGTACGGC CACACAAAGA TCTTTATACG CTCGCCACGC 
ACCCTGTTTG CCCTGGAGCA TCAGAGGAAC GAAATGATCC CACATATTGT GACGCTGCTG 
CAAAAGCGAG TGCGTGGATG GATTGTGAGG AGAAACTTTA AGAAGATGAA GGCGGCAATT 
ACGATCGTGC GCGCCTATAA GGCCTACAAA CTGCGTAGCT ACGTCCAGGA GCTGGCCAAT 
CGCTTACGGA AAGCCAAGCA GATGCGCGAC TACGGCAAGA GCATCCAGTG GCCACAGCCG 
CCGCTCGCCG GACGTAAGGT GGAGGCTAAA CTGCATCGTA TGTTCGATTT CTGGCGAGCC 
AACATGATCC TGCACAAATA TCCTCGCAGC GAGTGGCCGC AATTACGTCT GCAGATTATC 
GCCGCCACTG CGCTGGCCGG AAGACGACCC TATTGGGGTC AAGCGCGCCG CTGGGTGGGT 
GATTATCTGG CCAACTCGCA GGAGAACAGC GGATATGAAG CCTACAACGG AAGCATAAAG 
AACATAAGGA ACCACCCCGC CGACGGCGAG ACCTTCCAGC AAGTGCTCTT CTCCTCATTC 
GTCAAGAAGT TCAACCATTT CAACAAGCAG GCTAATCGCG CCTTCATCGT TTCCGATTCG 
ACCATCCACA AACTGGACGG TATCAAGAAT AAATTTAAGG ACATGAAACG CACCATTAAA 
ATTAGGGAGT TGACGTCCAT TAGTGTCTCA CCCGGCCGGG ATCAACTAAT CGTCTTCCAC 
TCGTCGAAAA ACAAGGATTT AGTGTTTTCC CTGGAGAGCG AGTACACGCC CCTCAAGGAA 
GATCGCATTG GCGAAGTGGT GGGCATTGTG TGCAAGAAAT ACCATGACCT CACTGGAACC 
GAGTTGCGTG TGAATGTGAC CACCAATATT TCCTGTCGCC TAGACGGCAA GGCACGCATT 
ATTACCGTGG AGGCGGCGTC CAATGTGGAG GTCCCCAACT TCCGTCCTAA AGAGGGCAAC 
ATCATTTTCG AGGTGCCGGC GGCGTACTGC GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523538.1
CDS	189..3224
Translation	MAMQREAGVQDFVLLDQVSMEKFMDNLRKRFQNGSIYTYIGEVCVSMNPYRQMNIYGPETIRKYKGRELFENAPHLFAIADSAYRVLKQRQQDTCILISGESGAGKTEASKIIMKYIAAVTNAQGQNEIERVKNVLIQSNAILETFGNAKTNRNDNSSRFGKYMDIEFDYKADPVGGIITNYLLEKSRVVQQQPGERNFHSFYQLLRGANDNELRQYELQKETGKYHYLNQGSMDILTEKSDYKGTCNAFKTLGFSTDEVQTIWRTIAAVLHLGNVEFQTIEDELVISNKQHLKSTAKLLQVTETELSTALTKRVIAAGGNVMQKDHNATQAEYGKDALAKAIYDRLFTWIISRINRAILFRGSKTQARFNSVIGVLDIYGFEIFDSNSFEQFCINYCNEKLQQLFIELVLKQEQEEYQREGIEWTNIEYFNNKIICDLVEQPHKGIIAIMDEACLSVGKVTDDTLLGAMDKNLSKHPHYTSRQLKPTDKELKHREDFRITHYAGDVIYNINGFIEKNKDTLYQDFKRLLHNSKDANLSEMWPEGAQDIKKTTKRPLTAGTLFQRSMADLVVTLLKKEPFYVRCIKPNDLKSSTVFDEERVEHQVRYLGLLENLRVRRAGFVHRQRYDKFLLRYKMISQYTWPNFRAGSDRDGVRVLIEEKKFAQDVKYGHTKIFIRSPRTLFALEHQRNEMIPHIVTLLQKRVRGWIVRRNFKKMKAAITIVRAYKAYKLRSYVQELANRLRKAKQMRDYGKSIQWPQPPLAGRKVEAKLHRMFDFWRANMILHKYPRSEWPQLRLQIIAATALAGRRPYWGQARRWVGDYLANSQENSGYEAYNGSIKNIRNHPADGETFQQVLFSSFVKKFNHFNKQANRAFIVSDSTIHKLDGIKNKFKDMKRTIKIRELTSISVSPGRDQLIVFHSSKNKDLVFSLESEYTPLKEDRIGEVVGIVCKKYHDLTGTELRVNVTTNISCRLDGKARIITVEAASNVEVPNFRPKEGNIIFEVPAAYCV

Target ORF information:

RefSeq Version	NM_078814.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin 31DF, transcript variant A (Myo31DF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078814.4

ORF Insert Sequence:

ATGGCTATGC AACGGGAAGC TGGAGTGCAG GACTTCGTCC TCCTCGACCA AGTGTCCATG 
GAGAAGTTCA TGGATAACCT GAGAAAGCGA TTCCAAAACG GATCCATCTA CACATACATC 
GGCGAAGTGT GCGTCTCGAT GAATCCCTAC AGACAGATGA ACATCTACGG TCCGGAAACC 
ATTCGCAAAT ACAAGGGTCG GGAGCTCTTC GAGAATGCCC CACATTTGTT CGCCATTGCC 
GATTCCGCCT ACAGGGTGCT CAAGCAGCGC CAACAAGACA CCTGCATCCT AATTTCCGGT 
GAGTCCGGTG CGGGCAAAAC GGAGGCCTCC AAGATCATCA TGAAGTACAT TGCGGCGGTG 
ACGAATGCCC AGGGTCAGAA CGAAATCGAG AGAGTCAAGA ACGTTTTGAT CCAGAGCAAT 
GCAATTCTGG AAACCTTCGG CAACGCAAAG ACTAACCGCA ACGACAACTC CAGCCGCTTC 
GGAAAGTACA TGGACATTGA GTTCGACTAC AAGGCGGATC CCGTGGGTGG CATCATCACC 
AACTATCTGC TCGAGAAGTC GCGCGTGGTG CAGCAGCAGC CCGGCGAGCG GAACTTCCAT 
AGTTTCTATC AGCTGCTGCG TGGAGCCAAC GACAACGAGC TGCGGCAATA TGAACTGCAG 
AAGGAAACGG GCAAGTATCA CTACTTGAAC CAGGGCAGCA TGGACATACT GACGGAGAAG 
TCCGACTACA AGGGCACCTG CAATGCCTTC AAGACCCTGG GCTTCTCCAC GGATGAGGTG 
CAGACCATTT GGCGCACAAT TGCCGCTGTC TTGCATCTGG GAAATGTGGA GTTCCAAACC 
ATTGAGGATG AGCTGGTGAT CAGCAATAAG CAGCACTTGA AATCCACGGC CAAGCTGCTC 
CAGGTCACCG AAACCGAGCT GTCCACGGCA CTGACCAAAC GGGTCATTGC GGCCGGTGGA 
AATGTGATGC AGAAGGATCA CAACGCCACG CAGGCAGAGT ACGGAAAGGA TGCTCTGGCC 
AAGGCCATCT ACGACCGTCT CTTCACCTGG ATCATCTCCC GCATCAACAG GGCAATCCTA 
TTCCGTGGCA GCAAGACGCA GGCGCGATTT AACTCGGTCA TCGGAGTGCT GGATATTTAC 
GGCTTCGAGA TCTTCGACAG CAACAGCTTT GAACAGTTCT GCATCAACTA TTGCAACGAG 
AAATTGCAGC AGTTATTCAT AGAACTGGTG CTTAAACAGG AGCAAGAGGA GTACCAGCGT 
GAGGGCATTG AATGGACCAA TATTGAATAC TTCAACAACA AGATCATTTG CGATCTCGTT 
GAGCAGCCGC ATAAGGGCAT CATTGCCATC ATGGACGAGG CCTGCCTCAG CGTGGGCAAG 
GTGACGGACG ACACACTTCT GGGGGCCATG GACAAAAATC TGAGCAAGCA TCCGCACTAC 
ACCAGTCGTC AGCTGAAGCC CACGGATAAG GAGCTAAAGC ATCGCGAGGA TTTCCGTATC 
ACCCACTACG CCGGCGATGT GATCTACAAC ATCAATGGAT TCATCGAGAA GAACAAGGAT 
ACGCTGTATC AGGACTTTAA GCGCTTGCTG CACAACTCTA AGGATGCCAA CCTGAGCGAG 
ATGTGGCCCG AAGGAGCACA GGATATCAAG AAAACCACCA AGAGACCATT GACAGCCGGC 
ACCTTGTTCC AGAGATCGAT GGCTGATCTG GTGGTTACTC TGCTGAAGAA GGAACCCTTC 
TATGTGCGCT GCATCAAGCC CAATGACCTT AAGAGCTCGA CAGTTTTCGA TGAGGAGCGT 
GTGGAGCACC AGGTGCGATA CCTTGGCCTA CTGGAGAACT TACGTGTCCG TCGAGCCGGA 
TTCGTTCATC GCCAGCGCTA TGATAAGTTC CTGCTGCGCT ACAAGATGAT CTCGCAGTAC 
ACTTGGCCCA ATTTCCGTGC GGGAAGCGAC CGCGATGGTG TCCGTGTGCT GATCGAGGAA 
AAGAAATTCG CCCAGGACGT TAAGTACGGC CACACAAAGA TCTTTATACG CTCGCCACGC 
ACCCTGTTTG CCCTGGAGCA TCAGAGGAAC GAAATGATCC CACATATTGT GACGCTGCTG 
CAAAAGCGAG TGCGTGGATG GATTGTGAGG AGAAACTTTA AGAAGATGAA GGCGGCAATT 
ACGATCGTGC GCGCCTATAA GGCCTACAAA CTGCGTAGCT ACGTCCAGGA GCTGGCCAAT 
CGCTTACGGA AAGCCAAGCA GATGCGCGAC TACGGCAAGA GCATCCAGTG GCCACAGCCG 
CCGCTCGCCG GACGTAAGGT GGAGGCTAAA CTGCATCGTA TGTTCGATTT CTGGCGAGCC 
AACATGATCC TGCACAAATA TCCTCGCAGC GAGTGGCCGC AATTACGTCT GCAGATTATC 
GCCGCCACTG CGCTGGCCGG AAGACGACCC TATTGGGGTC AAGCGCGCCG CTGGGTGGGT 
GATTATCTGG CCAACTCGCA GGAGAACAGC GGATATGAAG CCTACAACGG AAGCATAAAG 
AACATAAGGA ACCACCCCGC CGACGGCGAG ACCTTCCAGC AAGTGCTCTT CTCCTCATTC 
GTCAAGAAGT TCAACCATTT CAACAAGCAG GCTAATCGCG CCTTCATCGT TTCCGATTCG 
ACCATCCACA AACTGGACGG TATCAAGAAT AAATTTAAGG ACATGAAACG CACCATTAAA 
ATTAGGGAGT TGACGTCCAT TAGTGTCTCA CCCGGCCGGG ATCAACTAAT CGTCTTCCAC 
TCGTCGAAAA ACAAGGATTT AGTGTTTTCC CTGGAGAGCG AGTACACGCC CCTCAAGGAA 
GATCGCATTG GCGAAGTGGT GGGCATTGTG TGCAAGAAAT ACCATGACCT CACTGGAACC 
GAGTTGCGTG TGAATGTGAC CACCAATATT TCCTGTCGCC TAGACGGCAA GGCACGCATT 
ATTACCGTGG AGGCGGCGTC CAATGTGGAG GTCCCCAACT TCCGTCCTAA AGAGGGCAAC 
ATCATTTTCG AGGTGCCGGC GGCGTACTGC GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo31DF ( NM_164933.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723596.1 Drosophila melanogaster myosin 31DF, transcript variant B (Myo31DF), mRNA.

Price & Availability↑

CloneID	OFa14370
Clone ID Related Accession (Same CDS sequence)	NM_078814.4 , NM_164933.3 , NM_001201855.2
Accession Version	NM_164933.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin 31DF, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164933.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTATGC AACGGGAAGC TGGAGTGCAG GACTTCGTCC TCCTCGACCA AGTGTCCATG 
GAGAAGTTCA TGGATAACCT GAGAAAGCGA TTCCAAAACG GATCCATCTA CACATACATC 
GGCGAAGTGT GCGTCTCGAT GAATCCCTAC AGACAGATGA ACATCTACGG TCCGGAAACC 
ATTCGCAAAT ACAAGGGTCG GGAGCTCTTC GAGAATGCCC CACATTTGTT CGCCATTGCC 
GATTCCGCCT ACAGGGTGCT CAAGCAGCGC CAACAAGACA CCTGCATCCT AATTTCCGGT 
GAGTCCGGTG CGGGCAAAAC GGAGGCCTCC AAGATCATCA TGAAGTACAT TGCGGCGGTG 
ACGAATGCCC AGGGTCAGAA CGAAATCGAG AGAGTCAAGA ACGTTTTGAT CCAGAGCAAT 
GCAATTCTGG AAACCTTCGG CAACGCAAAG ACTAACCGCA ACGACAACTC CAGCCGCTTC 
GGAAAGTACA TGGACATTGA GTTCGACTAC AAGGCGGATC CCGTGGGTGG CATCATCACC 
AACTATCTGC TCGAGAAGTC GCGCGTGGTG CAGCAGCAGC CCGGCGAGCG GAACTTCCAT 
AGTTTCTATC AGCTGCTGCG TGGAGCCAAC GACAACGAGC TGCGGCAATA TGAACTGCAG 
AAGGAAACGG GCAAGTATCA CTACTTGAAC CAGGGCAGCA TGGACATACT GACGGAGAAG 
TCCGACTACA AGGGCACCTG CAATGCCTTC AAGACCCTGG GCTTCTCCAC GGATGAGGTG 
CAGACCATTT GGCGCACAAT TGCCGCTGTC TTGCATCTGG GAAATGTGGA GTTCCAAACC 
ATTGAGGATG AGCTGGTGAT CAGCAATAAG CAGCACTTGA AATCCACGGC CAAGCTGCTC 
CAGGTCACCG AAACCGAGCT GTCCACGGCA CTGACCAAAC GGGTCATTGC GGCCGGTGGA 
AATGTGATGC AGAAGGATCA CAACGCCACG CAGGCAGAGT ACGGAAAGGA TGCTCTGGCC 
AAGGCCATCT ACGACCGTCT CTTCACCTGG ATCATCTCCC GCATCAACAG GGCAATCCTA 
TTCCGTGGCA GCAAGACGCA GGCGCGATTT AACTCGGTCA TCGGAGTGCT GGATATTTAC 
GGCTTCGAGA TCTTCGACAG CAACAGCTTT GAACAGTTCT GCATCAACTA TTGCAACGAG 
AAATTGCAGC AGTTATTCAT AGAACTGGTG CTTAAACAGG AGCAAGAGGA GTACCAGCGT 
GAGGGCATTG AATGGACCAA TATTGAATAC TTCAACAACA AGATCATTTG CGATCTCGTT 
GAGCAGCCGC ATAAGGGCAT CATTGCCATC ATGGACGAGG CCTGCCTCAG CGTGGGCAAG 
GTGACGGACG ACACACTTCT GGGGGCCATG GACAAAAATC TGAGCAAGCA TCCGCACTAC 
ACCAGTCGTC AGCTGAAGCC CACGGATAAG GAGCTAAAGC ATCGCGAGGA TTTCCGTATC 
ACCCACTACG CCGGCGATGT GATCTACAAC ATCAATGGAT TCATCGAGAA GAACAAGGAT 
ACGCTGTATC AGGACTTTAA GCGCTTGCTG CACAACTCTA AGGATGCCAA CCTGAGCGAG 
ATGTGGCCCG AAGGAGCACA GGATATCAAG AAAACCACCA AGAGACCATT GACAGCCGGC 
ACCTTGTTCC AGAGATCGAT GGCTGATCTG GTGGTTACTC TGCTGAAGAA GGAACCCTTC 
TATGTGCGCT GCATCAAGCC CAATGACCTT AAGAGCTCGA CAGTTTTCGA TGAGGAGCGT 
GTGGAGCACC AGGTGCGATA CCTTGGCCTA CTGGAGAACT TACGTGTCCG TCGAGCCGGA 
TTCGTTCATC GCCAGCGCTA TGATAAGTTC CTGCTGCGCT ACAAGATGAT CTCGCAGTAC 
ACTTGGCCCA ATTTCCGTGC GGGAAGCGAC CGCGATGGTG TCCGTGTGCT GATCGAGGAA 
AAGAAATTCG CCCAGGACGT TAAGTACGGC CACACAAAGA TCTTTATACG CTCGCCACGC 
ACCCTGTTTG CCCTGGAGCA TCAGAGGAAC GAAATGATCC CACATATTGT GACGCTGCTG 
CAAAAGCGAG TGCGTGGATG GATTGTGAGG AGAAACTTTA AGAAGATGAA GGCGGCAATT 
ACGATCGTGC GCGCCTATAA GGCCTACAAA CTGCGTAGCT ACGTCCAGGA GCTGGCCAAT 
CGCTTACGGA AAGCCAAGCA GATGCGCGAC TACGGCAAGA GCATCCAGTG GCCACAGCCG 
CCGCTCGCCG GACGTAAGGT GGAGGCTAAA CTGCATCGTA TGTTCGATTT CTGGCGAGCC 
AACATGATCC TGCACAAATA TCCTCGCAGC GAGTGGCCGC AATTACGTCT GCAGATTATC 
GCCGCCACTG CGCTGGCCGG AAGACGACCC TATTGGGGTC AAGCGCGCCG CTGGGTGGGT 
GATTATCTGG CCAACTCGCA GGAGAACAGC GGATATGAAG CCTACAACGG AAGCATAAAG 
AACATAAGGA ACCACCCCGC CGACGGCGAG ACCTTCCAGC AAGTGCTCTT CTCCTCATTC 
GTCAAGAAGT TCAACCATTT CAACAAGCAG GCTAATCGCG CCTTCATCGT TTCCGATTCG 
ACCATCCACA AACTGGACGG TATCAAGAAT AAATTTAAGG ACATGAAACG CACCATTAAA 
ATTAGGGAGT TGACGTCCAT TAGTGTCTCA CCCGGCCGGG ATCAACTAAT CGTCTTCCAC 
TCGTCGAAAA ACAAGGATTT AGTGTTTTCC CTGGAGAGCG AGTACACGCC CCTCAAGGAA 
GATCGCATTG GCGAAGTGGT GGGCATTGTG TGCAAGAAAT ACCATGACCT CACTGGAACC 
GAGTTGCGTG TGAATGTGAC CACCAATATT TCCTGTCGCC TAGACGGCAA GGCACGCATT 
ATTACCGTGG AGGCGGCGTC CAATGTGGAG GTCCCCAACT TCCGTCCTAA AGAGGGCAAC 
ATCATTTTCG AGGTGCCGGC GGCGTACTGC GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723596.1
CDS	766..3801
Translation	MAMQREAGVQDFVLLDQVSMEKFMDNLRKRFQNGSIYTYIGEVCVSMNPYRQMNIYGPETIRKYKGRELFENAPHLFAIADSAYRVLKQRQQDTCILISGESGAGKTEASKIIMKYIAAVTNAQGQNEIERVKNVLIQSNAILETFGNAKTNRNDNSSRFGKYMDIEFDYKADPVGGIITNYLLEKSRVVQQQPGERNFHSFYQLLRGANDNELRQYELQKETGKYHYLNQGSMDILTEKSDYKGTCNAFKTLGFSTDEVQTIWRTIAAVLHLGNVEFQTIEDELVISNKQHLKSTAKLLQVTETELSTALTKRVIAAGGNVMQKDHNATQAEYGKDALAKAIYDRLFTWIISRINRAILFRGSKTQARFNSVIGVLDIYGFEIFDSNSFEQFCINYCNEKLQQLFIELVLKQEQEEYQREGIEWTNIEYFNNKIICDLVEQPHKGIIAIMDEACLSVGKVTDDTLLGAMDKNLSKHPHYTSRQLKPTDKELKHREDFRITHYAGDVIYNINGFIEKNKDTLYQDFKRLLHNSKDANLSEMWPEGAQDIKKTTKRPLTAGTLFQRSMADLVVTLLKKEPFYVRCIKPNDLKSSTVFDEERVEHQVRYLGLLENLRVRRAGFVHRQRYDKFLLRYKMISQYTWPNFRAGSDRDGVRVLIEEKKFAQDVKYGHTKIFIRSPRTLFALEHQRNEMIPHIVTLLQKRVRGWIVRRNFKKMKAAITIVRAYKAYKLRSYVQELANRLRKAKQMRDYGKSIQWPQPPLAGRKVEAKLHRMFDFWRANMILHKYPRSEWPQLRLQIIAATALAGRRPYWGQARRWVGDYLANSQENSGYEAYNGSIKNIRNHPADGETFQQVLFSSFVKKFNHFNKQANRAFIVSDSTIHKLDGIKNKFKDMKRTIKIRELTSISVSPGRDQLIVFHSSKNKDLVFSLESEYTPLKEDRIGEVVGIVCKKYHDLTGTELRVNVTTNISCRLDGKARIITVEAASNVEVPNFRPKEGNIIFEVPAAYCV

Target ORF information:

RefSeq Version	NM_164933.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin 31DF, transcript variant B (Myo31DF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164933.3

ORF Insert Sequence:

ATGGCTATGC AACGGGAAGC TGGAGTGCAG GACTTCGTCC TCCTCGACCA AGTGTCCATG 
GAGAAGTTCA TGGATAACCT GAGAAAGCGA TTCCAAAACG GATCCATCTA CACATACATC 
GGCGAAGTGT GCGTCTCGAT GAATCCCTAC AGACAGATGA ACATCTACGG TCCGGAAACC 
ATTCGCAAAT ACAAGGGTCG GGAGCTCTTC GAGAATGCCC CACATTTGTT CGCCATTGCC 
GATTCCGCCT ACAGGGTGCT CAAGCAGCGC CAACAAGACA CCTGCATCCT AATTTCCGGT 
GAGTCCGGTG CGGGCAAAAC GGAGGCCTCC AAGATCATCA TGAAGTACAT TGCGGCGGTG 
ACGAATGCCC AGGGTCAGAA CGAAATCGAG AGAGTCAAGA ACGTTTTGAT CCAGAGCAAT 
GCAATTCTGG AAACCTTCGG CAACGCAAAG ACTAACCGCA ACGACAACTC CAGCCGCTTC 
GGAAAGTACA TGGACATTGA GTTCGACTAC AAGGCGGATC CCGTGGGTGG CATCATCACC 
AACTATCTGC TCGAGAAGTC GCGCGTGGTG CAGCAGCAGC CCGGCGAGCG GAACTTCCAT 
AGTTTCTATC AGCTGCTGCG TGGAGCCAAC GACAACGAGC TGCGGCAATA TGAACTGCAG 
AAGGAAACGG GCAAGTATCA CTACTTGAAC CAGGGCAGCA TGGACATACT GACGGAGAAG 
TCCGACTACA AGGGCACCTG CAATGCCTTC AAGACCCTGG GCTTCTCCAC GGATGAGGTG 
CAGACCATTT GGCGCACAAT TGCCGCTGTC TTGCATCTGG GAAATGTGGA GTTCCAAACC 
ATTGAGGATG AGCTGGTGAT CAGCAATAAG CAGCACTTGA AATCCACGGC CAAGCTGCTC 
CAGGTCACCG AAACCGAGCT GTCCACGGCA CTGACCAAAC GGGTCATTGC GGCCGGTGGA 
AATGTGATGC AGAAGGATCA CAACGCCACG CAGGCAGAGT ACGGAAAGGA TGCTCTGGCC 
AAGGCCATCT ACGACCGTCT CTTCACCTGG ATCATCTCCC GCATCAACAG GGCAATCCTA 
TTCCGTGGCA GCAAGACGCA GGCGCGATTT AACTCGGTCA TCGGAGTGCT GGATATTTAC 
GGCTTCGAGA TCTTCGACAG CAACAGCTTT GAACAGTTCT GCATCAACTA TTGCAACGAG 
AAATTGCAGC AGTTATTCAT AGAACTGGTG CTTAAACAGG AGCAAGAGGA GTACCAGCGT 
GAGGGCATTG AATGGACCAA TATTGAATAC TTCAACAACA AGATCATTTG CGATCTCGTT 
GAGCAGCCGC ATAAGGGCAT CATTGCCATC ATGGACGAGG CCTGCCTCAG CGTGGGCAAG 
GTGACGGACG ACACACTTCT GGGGGCCATG GACAAAAATC TGAGCAAGCA TCCGCACTAC 
ACCAGTCGTC AGCTGAAGCC CACGGATAAG GAGCTAAAGC ATCGCGAGGA TTTCCGTATC 
ACCCACTACG CCGGCGATGT GATCTACAAC ATCAATGGAT TCATCGAGAA GAACAAGGAT 
ACGCTGTATC AGGACTTTAA GCGCTTGCTG CACAACTCTA AGGATGCCAA CCTGAGCGAG 
ATGTGGCCCG AAGGAGCACA GGATATCAAG AAAACCACCA AGAGACCATT GACAGCCGGC 
ACCTTGTTCC AGAGATCGAT GGCTGATCTG GTGGTTACTC TGCTGAAGAA GGAACCCTTC 
TATGTGCGCT GCATCAAGCC CAATGACCTT AAGAGCTCGA CAGTTTTCGA TGAGGAGCGT 
GTGGAGCACC AGGTGCGATA CCTTGGCCTA CTGGAGAACT TACGTGTCCG TCGAGCCGGA 
TTCGTTCATC GCCAGCGCTA TGATAAGTTC CTGCTGCGCT ACAAGATGAT CTCGCAGTAC 
ACTTGGCCCA ATTTCCGTGC GGGAAGCGAC CGCGATGGTG TCCGTGTGCT GATCGAGGAA 
AAGAAATTCG CCCAGGACGT TAAGTACGGC CACACAAAGA TCTTTATACG CTCGCCACGC 
ACCCTGTTTG CCCTGGAGCA TCAGAGGAAC GAAATGATCC CACATATTGT GACGCTGCTG 
CAAAAGCGAG TGCGTGGATG GATTGTGAGG AGAAACTTTA AGAAGATGAA GGCGGCAATT 
ACGATCGTGC GCGCCTATAA GGCCTACAAA CTGCGTAGCT ACGTCCAGGA GCTGGCCAAT 
CGCTTACGGA AAGCCAAGCA GATGCGCGAC TACGGCAAGA GCATCCAGTG GCCACAGCCG 
CCGCTCGCCG GACGTAAGGT GGAGGCTAAA CTGCATCGTA TGTTCGATTT CTGGCGAGCC 
AACATGATCC TGCACAAATA TCCTCGCAGC GAGTGGCCGC AATTACGTCT GCAGATTATC 
GCCGCCACTG CGCTGGCCGG AAGACGACCC TATTGGGGTC AAGCGCGCCG CTGGGTGGGT 
GATTATCTGG CCAACTCGCA GGAGAACAGC GGATATGAAG CCTACAACGG AAGCATAAAG 
AACATAAGGA ACCACCCCGC CGACGGCGAG ACCTTCCAGC AAGTGCTCTT CTCCTCATTC 
GTCAAGAAGT TCAACCATTT CAACAAGCAG GCTAATCGCG CCTTCATCGT TTCCGATTCG 
ACCATCCACA AACTGGACGG TATCAAGAAT AAATTTAAGG ACATGAAACG CACCATTAAA 
ATTAGGGAGT TGACGTCCAT TAGTGTCTCA CCCGGCCGGG ATCAACTAAT CGTCTTCCAC 
TCGTCGAAAA ACAAGGATTT AGTGTTTTCC CTGGAGAGCG AGTACACGCC CCTCAAGGAA 
GATCGCATTG GCGAAGTGGT GGGCATTGTG TGCAAGAAAT ACCATGACCT CACTGGAACC 
GAGTTGCGTG TGAATGTGAC CACCAATATT TCCTGTCGCC TAGACGGCAA GGCACGCATT 
ATTACCGTGG AGGCGGCGTC CAATGTGGAG GTCCCCAACT TCCGTCCTAA AGAGGGCAAC 
ATCATTTTCG AGGTGCCGGC GGCGTACTGC GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo31DF ( NM_001201855.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188784.1 Drosophila melanogaster myosin 31DF, transcript variant C (Myo31DF), mRNA.

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CloneID	OFa14370
Clone ID Related Accession (Same CDS sequence)	NM_078814.4 , NM_164933.3 , NM_001201855.2
Accession Version	NM_001201855.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin 31DF, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001201855.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTATGC AACGGGAAGC TGGAGTGCAG GACTTCGTCC TCCTCGACCA AGTGTCCATG 
GAGAAGTTCA TGGATAACCT GAGAAAGCGA TTCCAAAACG GATCCATCTA CACATACATC 
GGCGAAGTGT GCGTCTCGAT GAATCCCTAC AGACAGATGA ACATCTACGG TCCGGAAACC 
ATTCGCAAAT ACAAGGGTCG GGAGCTCTTC GAGAATGCCC CACATTTGTT CGCCATTGCC 
GATTCCGCCT ACAGGGTGCT CAAGCAGCGC CAACAAGACA CCTGCATCCT AATTTCCGGT 
GAGTCCGGTG CGGGCAAAAC GGAGGCCTCC AAGATCATCA TGAAGTACAT TGCGGCGGTG 
ACGAATGCCC AGGGTCAGAA CGAAATCGAG AGAGTCAAGA ACGTTTTGAT CCAGAGCAAT 
GCAATTCTGG AAACCTTCGG CAACGCAAAG ACTAACCGCA ACGACAACTC CAGCCGCTTC 
GGAAAGTACA TGGACATTGA GTTCGACTAC AAGGCGGATC CCGTGGGTGG CATCATCACC 
AACTATCTGC TCGAGAAGTC GCGCGTGGTG CAGCAGCAGC CCGGCGAGCG GAACTTCCAT 
AGTTTCTATC AGCTGCTGCG TGGAGCCAAC GACAACGAGC TGCGGCAATA TGAACTGCAG 
AAGGAAACGG GCAAGTATCA CTACTTGAAC CAGGGCAGCA TGGACATACT GACGGAGAAG 
TCCGACTACA AGGGCACCTG CAATGCCTTC AAGACCCTGG GCTTCTCCAC GGATGAGGTG 
CAGACCATTT GGCGCACAAT TGCCGCTGTC TTGCATCTGG GAAATGTGGA GTTCCAAACC 
ATTGAGGATG AGCTGGTGAT CAGCAATAAG CAGCACTTGA AATCCACGGC CAAGCTGCTC 
CAGGTCACCG AAACCGAGCT GTCCACGGCA CTGACCAAAC GGGTCATTGC GGCCGGTGGA 
AATGTGATGC AGAAGGATCA CAACGCCACG CAGGCAGAGT ACGGAAAGGA TGCTCTGGCC 
AAGGCCATCT ACGACCGTCT CTTCACCTGG ATCATCTCCC GCATCAACAG GGCAATCCTA 
TTCCGTGGCA GCAAGACGCA GGCGCGATTT AACTCGGTCA TCGGAGTGCT GGATATTTAC 
GGCTTCGAGA TCTTCGACAG CAACAGCTTT GAACAGTTCT GCATCAACTA TTGCAACGAG 
AAATTGCAGC AGTTATTCAT AGAACTGGTG CTTAAACAGG AGCAAGAGGA GTACCAGCGT 
GAGGGCATTG AATGGACCAA TATTGAATAC TTCAACAACA AGATCATTTG CGATCTCGTT 
GAGCAGCCGC ATAAGGGCAT CATTGCCATC ATGGACGAGG CCTGCCTCAG CGTGGGCAAG 
GTGACGGACG ACACACTTCT GGGGGCCATG GACAAAAATC TGAGCAAGCA TCCGCACTAC 
ACCAGTCGTC AGCTGAAGCC CACGGATAAG GAGCTAAAGC ATCGCGAGGA TTTCCGTATC 
ACCCACTACG CCGGCGATGT GATCTACAAC ATCAATGGAT TCATCGAGAA GAACAAGGAT 
ACGCTGTATC AGGACTTTAA GCGCTTGCTG CACAACTCTA AGGATGCCAA CCTGAGCGAG 
ATGTGGCCCG AAGGAGCACA GGATATCAAG AAAACCACCA AGAGACCATT GACAGCCGGC 
ACCTTGTTCC AGAGATCGAT GGCTGATCTG GTGGTTACTC TGCTGAAGAA GGAACCCTTC 
TATGTGCGCT GCATCAAGCC CAATGACCTT AAGAGCTCGA CAGTTTTCGA TGAGGAGCGT 
GTGGAGCACC AGGTGCGATA CCTTGGCCTA CTGGAGAACT TACGTGTCCG TCGAGCCGGA 
TTCGTTCATC GCCAGCGCTA TGATAAGTTC CTGCTGCGCT ACAAGATGAT CTCGCAGTAC 
ACTTGGCCCA ATTTCCGTGC GGGAAGCGAC CGCGATGGTG TCCGTGTGCT GATCGAGGAA 
AAGAAATTCG CCCAGGACGT TAAGTACGGC CACACAAAGA TCTTTATACG CTCGCCACGC 
ACCCTGTTTG CCCTGGAGCA TCAGAGGAAC GAAATGATCC CACATATTGT GACGCTGCTG 
CAAAAGCGAG TGCGTGGATG GATTGTGAGG AGAAACTTTA AGAAGATGAA GGCGGCAATT 
ACGATCGTGC GCGCCTATAA GGCCTACAAA CTGCGTAGCT ACGTCCAGGA GCTGGCCAAT 
CGCTTACGGA AAGCCAAGCA GATGCGCGAC TACGGCAAGA GCATCCAGTG GCCACAGCCG 
CCGCTCGCCG GACGTAAGGT GGAGGCTAAA CTGCATCGTA TGTTCGATTT CTGGCGAGCC 
AACATGATCC TGCACAAATA TCCTCGCAGC GAGTGGCCGC AATTACGTCT GCAGATTATC 
GCCGCCACTG CGCTGGCCGG AAGACGACCC TATTGGGGTC AAGCGCGCCG CTGGGTGGGT 
GATTATCTGG CCAACTCGCA GGAGAACAGC GGATATGAAG CCTACAACGG AAGCATAAAG 
AACATAAGGA ACCACCCCGC CGACGGCGAG ACCTTCCAGC AAGTGCTCTT CTCCTCATTC 
GTCAAGAAGT TCAACCATTT CAACAAGCAG GCTAATCGCG CCTTCATCGT TTCCGATTCG 
ACCATCCACA AACTGGACGG TATCAAGAAT AAATTTAAGG ACATGAAACG CACCATTAAA 
ATTAGGGAGT TGACGTCCAT TAGTGTCTCA CCCGGCCGGG ATCAACTAAT CGTCTTCCAC 
TCGTCGAAAA ACAAGGATTT AGTGTTTTCC CTGGAGAGCG AGTACACGCC CCTCAAGGAA 
GATCGCATTG GCGAAGTGGT GGGCATTGTG TGCAAGAAAT ACCATGACCT CACTGGAACC 
GAGTTGCGTG TGAATGTGAC CACCAATATT TCCTGTCGCC TAGACGGCAA GGCACGCATT 
ATTACCGTGG AGGCGGCGTC CAATGTGGAG GTCCCCAACT TCCGTCCTAA AGAGGGCAAC 
ATCATTTTCG AGGTGCCGGC GGCGTACTGC GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188784.1
CDS	330..3365
Translation	MAMQREAGVQDFVLLDQVSMEKFMDNLRKRFQNGSIYTYIGEVCVSMNPYRQMNIYGPETIRKYKGRELFENAPHLFAIADSAYRVLKQRQQDTCILISGESGAGKTEASKIIMKYIAAVTNAQGQNEIERVKNVLIQSNAILETFGNAKTNRNDNSSRFGKYMDIEFDYKADPVGGIITNYLLEKSRVVQQQPGERNFHSFYQLLRGANDNELRQYELQKETGKYHYLNQGSMDILTEKSDYKGTCNAFKTLGFSTDEVQTIWRTIAAVLHLGNVEFQTIEDELVISNKQHLKSTAKLLQVTETELSTALTKRVIAAGGNVMQKDHNATQAEYGKDALAKAIYDRLFTWIISRINRAILFRGSKTQARFNSVIGVLDIYGFEIFDSNSFEQFCINYCNEKLQQLFIELVLKQEQEEYQREGIEWTNIEYFNNKIICDLVEQPHKGIIAIMDEACLSVGKVTDDTLLGAMDKNLSKHPHYTSRQLKPTDKELKHREDFRITHYAGDVIYNINGFIEKNKDTLYQDFKRLLHNSKDANLSEMWPEGAQDIKKTTKRPLTAGTLFQRSMADLVVTLLKKEPFYVRCIKPNDLKSSTVFDEERVEHQVRYLGLLENLRVRRAGFVHRQRYDKFLLRYKMISQYTWPNFRAGSDRDGVRVLIEEKKFAQDVKYGHTKIFIRSPRTLFALEHQRNEMIPHIVTLLQKRVRGWIVRRNFKKMKAAITIVRAYKAYKLRSYVQELANRLRKAKQMRDYGKSIQWPQPPLAGRKVEAKLHRMFDFWRANMILHKYPRSEWPQLRLQIIAATALAGRRPYWGQARRWVGDYLANSQENSGYEAYNGSIKNIRNHPADGETFQQVLFSSFVKKFNHFNKQANRAFIVSDSTIHKLDGIKNKFKDMKRTIKIRELTSISVSPGRDQLIVFHSSKNKDLVFSLESEYTPLKEDRIGEVVGIVCKKYHDLTGTELRVNVTTNISCRLDGKARIITVEAASNVEVPNFRPKEGNIIFEVPAAYCV

Target ORF information:

RefSeq Version	NM_001201855.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin 31DF, transcript variant C (Myo31DF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201855.2

ORF Insert Sequence:

ATGGCTATGC AACGGGAAGC TGGAGTGCAG GACTTCGTCC TCCTCGACCA AGTGTCCATG 
GAGAAGTTCA TGGATAACCT GAGAAAGCGA TTCCAAAACG GATCCATCTA CACATACATC 
GGCGAAGTGT GCGTCTCGAT GAATCCCTAC AGACAGATGA ACATCTACGG TCCGGAAACC 
ATTCGCAAAT ACAAGGGTCG GGAGCTCTTC GAGAATGCCC CACATTTGTT CGCCATTGCC 
GATTCCGCCT ACAGGGTGCT CAAGCAGCGC CAACAAGACA CCTGCATCCT AATTTCCGGT 
GAGTCCGGTG CGGGCAAAAC GGAGGCCTCC AAGATCATCA TGAAGTACAT TGCGGCGGTG 
ACGAATGCCC AGGGTCAGAA CGAAATCGAG AGAGTCAAGA ACGTTTTGAT CCAGAGCAAT 
GCAATTCTGG AAACCTTCGG CAACGCAAAG ACTAACCGCA ACGACAACTC CAGCCGCTTC 
GGAAAGTACA TGGACATTGA GTTCGACTAC AAGGCGGATC CCGTGGGTGG CATCATCACC 
AACTATCTGC TCGAGAAGTC GCGCGTGGTG CAGCAGCAGC CCGGCGAGCG GAACTTCCAT 
AGTTTCTATC AGCTGCTGCG TGGAGCCAAC GACAACGAGC TGCGGCAATA TGAACTGCAG 
AAGGAAACGG GCAAGTATCA CTACTTGAAC CAGGGCAGCA TGGACATACT GACGGAGAAG 
TCCGACTACA AGGGCACCTG CAATGCCTTC AAGACCCTGG GCTTCTCCAC GGATGAGGTG 
CAGACCATTT GGCGCACAAT TGCCGCTGTC TTGCATCTGG GAAATGTGGA GTTCCAAACC 
ATTGAGGATG AGCTGGTGAT CAGCAATAAG CAGCACTTGA AATCCACGGC CAAGCTGCTC 
CAGGTCACCG AAACCGAGCT GTCCACGGCA CTGACCAAAC GGGTCATTGC GGCCGGTGGA 
AATGTGATGC AGAAGGATCA CAACGCCACG CAGGCAGAGT ACGGAAAGGA TGCTCTGGCC 
AAGGCCATCT ACGACCGTCT CTTCACCTGG ATCATCTCCC GCATCAACAG GGCAATCCTA 
TTCCGTGGCA GCAAGACGCA GGCGCGATTT AACTCGGTCA TCGGAGTGCT GGATATTTAC 
GGCTTCGAGA TCTTCGACAG CAACAGCTTT GAACAGTTCT GCATCAACTA TTGCAACGAG 
AAATTGCAGC AGTTATTCAT AGAACTGGTG CTTAAACAGG AGCAAGAGGA GTACCAGCGT 
GAGGGCATTG AATGGACCAA TATTGAATAC TTCAACAACA AGATCATTTG CGATCTCGTT 
GAGCAGCCGC ATAAGGGCAT CATTGCCATC ATGGACGAGG CCTGCCTCAG CGTGGGCAAG 
GTGACGGACG ACACACTTCT GGGGGCCATG GACAAAAATC TGAGCAAGCA TCCGCACTAC 
ACCAGTCGTC AGCTGAAGCC CACGGATAAG GAGCTAAAGC ATCGCGAGGA TTTCCGTATC 
ACCCACTACG CCGGCGATGT GATCTACAAC ATCAATGGAT TCATCGAGAA GAACAAGGAT 
ACGCTGTATC AGGACTTTAA GCGCTTGCTG CACAACTCTA AGGATGCCAA CCTGAGCGAG 
ATGTGGCCCG AAGGAGCACA GGATATCAAG AAAACCACCA AGAGACCATT GACAGCCGGC 
ACCTTGTTCC AGAGATCGAT GGCTGATCTG GTGGTTACTC TGCTGAAGAA GGAACCCTTC 
TATGTGCGCT GCATCAAGCC CAATGACCTT AAGAGCTCGA CAGTTTTCGA TGAGGAGCGT 
GTGGAGCACC AGGTGCGATA CCTTGGCCTA CTGGAGAACT TACGTGTCCG TCGAGCCGGA 
TTCGTTCATC GCCAGCGCTA TGATAAGTTC CTGCTGCGCT ACAAGATGAT CTCGCAGTAC 
ACTTGGCCCA ATTTCCGTGC GGGAAGCGAC CGCGATGGTG TCCGTGTGCT GATCGAGGAA 
AAGAAATTCG CCCAGGACGT TAAGTACGGC CACACAAAGA TCTTTATACG CTCGCCACGC 
ACCCTGTTTG CCCTGGAGCA TCAGAGGAAC GAAATGATCC CACATATTGT GACGCTGCTG 
CAAAAGCGAG TGCGTGGATG GATTGTGAGG AGAAACTTTA AGAAGATGAA GGCGGCAATT 
ACGATCGTGC GCGCCTATAA GGCCTACAAA CTGCGTAGCT ACGTCCAGGA GCTGGCCAAT 
CGCTTACGGA AAGCCAAGCA GATGCGCGAC TACGGCAAGA GCATCCAGTG GCCACAGCCG 
CCGCTCGCCG GACGTAAGGT GGAGGCTAAA CTGCATCGTA TGTTCGATTT CTGGCGAGCC 
AACATGATCC TGCACAAATA TCCTCGCAGC GAGTGGCCGC AATTACGTCT GCAGATTATC 
GCCGCCACTG CGCTGGCCGG AAGACGACCC TATTGGGGTC AAGCGCGCCG CTGGGTGGGT 
GATTATCTGG CCAACTCGCA GGAGAACAGC GGATATGAAG CCTACAACGG AAGCATAAAG 
AACATAAGGA ACCACCCCGC CGACGGCGAG ACCTTCCAGC AAGTGCTCTT CTCCTCATTC 
GTCAAGAAGT TCAACCATTT CAACAAGCAG GCTAATCGCG CCTTCATCGT TTCCGATTCG 
ACCATCCACA AACTGGACGG TATCAAGAAT AAATTTAAGG ACATGAAACG CACCATTAAA 
ATTAGGGAGT TGACGTCCAT TAGTGTCTCA CCCGGCCGGG ATCAACTAAT CGTCTTCCAC 
TCGTCGAAAA ACAAGGATTT AGTGTTTTCC CTGGAGAGCG AGTACACGCC CCTCAAGGAA 
GATCGCATTG GCGAAGTGGT GGGCATTGTG TGCAAGAAAT ACCATGACCT CACTGGAACC 
GAGTTGCGTG TGAATGTGAC CACCAATATT TCCTGTCGCC TAGACGGCAA GGCACGCATT 
ATTACCGTGG AGGCGGCGTC CAATGTGGAG GTCCCCAACT TCCGTCCTAA AGAGGGCAAC 
ATCATTTTCG AGGTGCCGGC GGCGTACTGC GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Myo31DF cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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