Cdk1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cdk1
Entrez Gene ID	34411
Full Name	Cyclin-dependent kinase 1
Synonyms	5363,CDC2,CDCDm,CDK1,CDK1/CDC2,CG5363,Cdc2,Cdk-1,Dcdc2,Dm cdc2,DmCdc2,DmCdk1,Dmcdc2,Dmel\CG5363,cdc,cdc2,cdc2Dm,cdk1,dCdk1,group 4,l(2)31Eh
General protein information	Preferred Names Cyclin-dependent kinase 1 Names CG5363 gene product from transcript CG5363-RA CG5363-PA Cdk1-PA cdc2 cdc2 kinase cell division control cyclin dependent kinase cyclin-dependent kinase protein 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 31D11-31D11

mRNA and Protein(s)

mRNA	Protein	Name
NM_057449.4	NP_476797.1	Cyclin-dependent kinase 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CDK1	XP_001164774.1
	CDK1	XP_002805757.1
Bos taurus (cattle)	CDK1	NP_776441.1
Drosophila melanogaster (fruit fly)	cdc2	NP_476797.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009459	XP_307878.4
Caenorhabditis elegans (roundworm)	cdk-1	NP_001022747.1
Rattus norvegicus (Norway rat)	Cdk1	NP_062169.1
Danio rerio (zebrafish)	cdk1	NP_997729.1
Homo sapiens (human)	CDK1	NP_001777.1
Xenopus tropicalis (tropical clawed frog)	cdk1	NP_988908.1
Canis lupus familiaris (dog)	CDK1	XP_005618958.1
Mus musculus (house mouse)	Cdk1	NP_031685.2
Gallus gallus (chicken)	CDK1	NP_990645.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cdk1 waves are not controlled by the mitotic switch but by a double-negative feedback between Cdk1 and Chk1. In Drosophila embryos, Cdk1 positive feedback serves primarily to ensure the rapid onset of mitosis, while wave propagation is regulated by S phase events.
Title: Waves of Cdk1 Activity in S Phase Synchronize the Cell Cycle in Drosophila Embryos.

Cdk1 phosphorylates the conserved centriole protein Sas-4 during mitosis. This creates a Polo-docking site that helps recruit Polo to daughter centrioles.
Title: Cdk1 Phosphorylates Drosophila Sas-4 to Recruit Polo to Daughter Centrioles and Convert Them to Centrosomes.

Our results indicate that the cyclic changes in Gwl localization at mitotic entry and exit are directly regulated by the antagonistic cyclin B-Cdk1 and PP2A-Tws enzymes
Title: Spatial regulation of greatwall by Cdk1 and PP2A-Tws in the cell cycle.

Cdk1 mediates the role of TARA and CycA in sleep regulation.
Title: TARANIS Functions with Cyclin A and Cdk1 in a Novel Arousal Center to Control Sleep in Drosophila.

Y15 phosphorylation can both inhibit Cdk1 catalytic activity and de-stabilize Cdk1/Cyclin complexes, whereas T161 phosphorylation facilitates stable interactions between cyclin B and Cdk1.
Title: "Ready, set, go": checkpoint regulation by Cdk1 inhibitory phosphorylation.

The following Cdk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cdk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14340	NM_057449.4 Latest version!	Drosophila melanogaster Cyclin-dependent kinase 1 (Cdk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cdk1 ( NM_057449.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476797.1 Drosophila melanogaster Cyclin-dependent kinase 1 (Cdk1), mRNA.

Price & Availability↑

CloneID	OFa14340
Clone ID Related Accession (Same CDS sequence)	NM_057449.4
Accession Version	NM_057449.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Cyclin-dependent kinase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057449.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGATT TTGAGAAAAT TGAGAAGATT GGCGAGGGCA CATATGGCGT GGTGTATAAG 
GGTCGCAACC GCCTGACGGG CCAAATTGTG GCAATGAAGA AAATCCGCTT GGAGTCCGAC 
GACGAAGGCG TTCCATCAAC CGCGATCAGA GAAATTTCGT TGCTTAAGGA GTTGAAACAT 
GAAAACATTG TCTGTTTGGA GGATGTTTTG ATGGAGGAGA ACCGCATATA CTTGATCTTT 
GAATTCCTAT CGATGGACCT CAAGAAATAC ATGGATTCGC TGCCAGTTGA TAAGCACATG 
GAGAGTGAAT TGGTCCGTAG CTATTTGTAC CAAATAACTA GCGCCATTCT TTTCTGCCAT 
CGTCGGCGAG TACTTCACCG TGATCTTAAG CCGCAGAACT TACTAATCGA CAAGAGTGGC 
CTCATAAAAG TCGCCGACTT TGGACTTGGC CGATCCTTTG GCATTCCGGT GCGCATTTAT 
ACGCACGAGA TTGTTACCTT GTGGTACAGA GCGCCGGAGG TGCTACTGGG TTCACCCCGG 
TATTCCTGTC CCGTCGATAT CTGGTCCATT GGATGCATAT TCGCGGAGAT GGCAACGAGA 
AAGCCGCTAT TCCAGGGTGA CTCGGAAATT GACCAGTTGT TTAGAATGTT TAGAATTCTG 
AAAACACCTA CCGAAGACAT TTGGCCGGGC GTTACTTCGC TACCCGACTA TAAGAACACG 
TTCCCCTGCT GGTCCACGAA CCAATTGACC AATCAGTTAA AGAATCTCGA TGCGAATGGT 
ATTGATCTCA TACAAAAGAT GTTAATCTAC GATCCAGTTC ATCGCATTTC CGCCAAGGAC 
ATTTTGGAGC ATCCCTATTT CAATGGTTTT CAATCGGGCT TAGTTCGAAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476797.1
CDS	86..979
Translation	MEDFEKIEKIGEGTYGVVYKGRNRLTGQIVAMKKIRLESDDEGVPSTAIREISLLKELKHENIVCLEDVLMEENRIYLIFEFLSMDLKKYMDSLPVDKHMESELVRSYLYQITSAILFCHRRRVLHRDLKPQNLLIDKSGLIKVADFGLGRSFGIPVRIYTHEIVTLWYRAPEVLLGSPRYSCPVDIWSIGCIFAEMATRKPLFQGDSEIDQLFRMFRILKTPTEDIWPGVTSLPDYKNTFPCWSTNQLTNQLKNLDANGIDLIQKMLIYDPVHRISAKDILEHPYFNGFQSGLVRN

Target ORF information:

RefSeq Version	NM_057449.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Cyclin-dependent kinase 1 (Cdk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057449.4

ORF Insert Sequence:

ATGGAGGATT TTGAGAAAAT TGAGAAGATT GGCGAGGGCA CATATGGCGT GGTGTATAAG 
GGTCGCAACC GCCTGACGGG CCAAATTGTG GCAATGAAGA AAATCCGCTT GGAGTCCGAC 
GACGAAGGCG TTCCATCAAC CGCGATCAGA GAAATTTCGT TGCTTAAGGA GTTGAAACAT 
GAAAACATTG TCTGTTTGGA GGATGTTTTG ATGGAGGAGA ACCGCATATA CTTGATCTTT 
GAATTCCTAT CGATGGACCT CAAGAAATAC ATGGATTCGC TGCCAGTTGA TAAGCACATG 
GAGAGTGAAT TGGTCCGTAG CTATTTGTAC CAAATAACTA GCGCCATTCT TTTCTGCCAT 
CGTCGGCGAG TACTTCACCG TGATCTTAAG CCGCAGAACT TACTAATCGA CAAGAGTGGC 
CTCATAAAAG TCGCCGACTT TGGACTTGGC CGATCCTTTG GCATTCCGGT GCGCATTTAT 
ACGCACGAGA TTGTTACCTT GTGGTACAGA GCGCCGGAGG TGCTACTGGG TTCACCCCGG 
TATTCCTGTC CCGTCGATAT CTGGTCCATT GGATGCATAT TCGCGGAGAT GGCAACGAGA 
AAGCCGCTAT TCCAGGGTGA CTCGGAAATT GACCAGTTGT TTAGAATGTT TAGAATTCTG 
AAAACACCTA CCGAAGACAT TTGGCCGGGC GTTACTTCGC TACCCGACTA TAAGAACACG 
TTCCCCTGCT GGTCCACGAA CCAATTGACC AATCAGTTAA AGAATCTCGA TGCGAATGGT 
ATTGATCTCA TACAAAAGAT GTTAATCTAC GATCCAGTTC ATCGCATTTC CGCCAAGGAC 
ATTTTGGAGC ATCCCTATTT CAATGGTTTT CAATCGGGCT TAGTTCGAAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Cdk1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報