Akap200 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Akap200
Entrez Gene ID	34170
Full Name	A kinase anchor protein 200
Synonyms	AKAP200,Akap 200,BcDNA:LP02734,CG13388,DAKAP200,Dmel\CG13388,EP(2)2254,MESR2,akap200,anon-WO0118547.134,anon-WO0118547.213,dpo
General protein information	Preferred Names A kinase anchor protein 200 Names CG13388 gene product from transcript CG13388-RD A kinase anchor protein Akap200-PA Akap200-PB Akap200-PC Akap200-PD Akap200-PE Akap200-PF Akap200-PG CG13388-PA CG13388-PB CG13388-PC CG13388-PD CG13388-PE CG13388-PF CG13388-PG
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 29C3-29C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_164820.2	NP_723395.1	A kinase anchor protein 200, isoform C
NM_001298823.1	NP_001285752.1	A kinase anchor protein 200, isoform F
NM_058112.4	NP_477460.1	A kinase anchor protein 200, isoform A
NM_175984.1	NP_787998.1	A kinase anchor protein 200, isoform D
NM_058111.4	NP_477459.1	A kinase anchor protein 200, isoform B
NM_001298822.1	NP_001285751.1	A kinase anchor protein 200, isoform E
NM_001298824.1	NP_001285753.1	A kinase anchor protein 200, isoform G

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Overexpression of Akap200 not only suppresses the phenotype caused by Dv-cbl expression, but also the severe disruption to eye development caused by the combined expression of Dv-cbl and activated Ras
Title: Akap200 suppresses the effects of Dv-cbl expression in the Drosophila eye.

Overexpression of Akap200 resulted in fusion of antennal glomeruli, while its loss resulted in misshapen and ectopic glomeruli.
Title: Misexpression screen for genes altering the olfactory map in Drosophila.

Akap200 is required for protein kinase A regulatory subunit localization and morphology of actin structures during oogenesis in Drosophila
Title: An A-kinase anchoring protein is required for protein kinase A regulatory subunit localization and morphology of actin structures during oogenesis in Drosophila.

The following Akap200 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Akap200 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa16479	NM_001298823.1 Latest version!	Drosophila melanogaster A kinase anchor protein 200 (Akap200), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa16479	NM_058111.4 Latest version!	Drosophila melanogaster A kinase anchor protein 200 (Akap200), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa16479	NM_164820.2 Latest version!	Drosophila melanogaster A kinase anchor protein 200 (Akap200), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa16477	NM_058112.4 Latest version!	Drosophila melanogaster A kinase anchor protein 200 (Akap200), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa16477	NM_001298822.1 Latest version!	Drosophila melanogaster A kinase anchor protein 200 (Akap200), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa16478	NM_001298824.1 Latest version!	Drosophila melanogaster A kinase anchor protein 200 (Akap200), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa16478	NM_175984.1 Latest version!	Drosophila melanogaster A kinase anchor protein 200 (Akap200), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Akap200 ( NM_164820.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723395.1 Drosophila melanogaster A kinase anchor protein 200 (Akap200), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa16479
Clone ID Related Accession (Same CDS sequence)	NM_164820.2 , NM_001298823.1 , NM_058111.4
Accession Version	NM_164820.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	A kinase anchor protein 200, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_164820.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTAAAG CTCAGAGCAA GCGTTCAATC GACATCACCA CCGATCCCAA GAAGGTCGGA 
GAGGGCGATG AGGTCGCCGG CAAGGTGGAG AAAATCGATG TGGACCAGAA GACGGACGCG 
CCGGCTGTGA ACGGAGACGC TGCCACGCCC AAAGAGGGTG GTGATGAAGC GGCGGCGGTA 
GAGAAAAAGG AAACTGAGGA GCATTCTGAG AACGACAAAG ATCTGACCAC CGAAAAGAGT 
GCCGCAGTCG CTGAGGGTGG TGATGCGGTC GCTGAAACGG CCAAGGGCGA GGAGGGATCT 
CCCAAGGAGG CTGCTGCTGG TGAGGATATA ACCCCGCTGG CAGACGAAAG CATCAAGTCC 
AAGTCCAAGA AGGACAAGGT CAAGAAGAAG TGGTCCTTCC GAAGCATTTC CTTTGGCAAG 
AAGGACAAAC AGAAACCGGC CAAGTCGGAG GAGGCTACGT CGCCCACCTC TGGCACAACG 
TCGCCCACGA CGGCCGAGGC AGAGGCTGCT CCAGCTGGGG ATGCAGCTGT CGCAGAACCA 
TCGGTGGCTA CAAATGGCGA GGCTGAAAAG CCAGCTGAGA CTGCCACTGC CACCAGCGAG 
CCGGCGTCCA AGGATGAGAA GCCCGCCGAA AATGGATCAG CGACCGAGCA GGAGAAACAG 
GCCAACGGAG AAACCGAAAA GGCGGCGCCA GCACCTTCCA CAGTTGAGGA AGCGGCGAAG 
CCCAAGCCCG CCGAAGAACC AGCCACAGTC ACTGCCACCG AATCGAACAC CACTGCCACC 
GAAGAAGTTC CCGTGAAAGA GAGCCAGCCA GAGCCCGAAG TGGTGACCAA TGGACATGGA 
GCCGGAGAGG CGCTGACCAA CGGATCAAGC AATGGACTGG CCGAGTCCCC AGTGACCGAG 
ACAGCACCTG TCGCTGACAA CATTCCGAGC AACGTTGATG ACGAGCCACC GCACCAGAAT 
GGCACCAATG GCACCACCAC GCCCCCGCCC ACTCCCGTGG CCACCGAGAT CGAGAAGGGA 
CAGCAAATTG AGGTGGATCT CGCCAAGGAT CTGAAGGAGA AGAATGCCGC CGCGGCAGAC 
GTTACAACAC AGGAACAACT GCCCGTTACA TGCGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723395.1
CDS	231..1349
Translation	MGKAQSKRSIDITTDPKKVGEGDEVAGKVEKIDVDQKTDAPAVNGDAATPKEGGDEAAAVEKKETEEHSENDKDLTTEKSAAVAEGGDAVAETAKGEEGSPKEAAAGEDITPLADESIKSKSKKDKVKKKWSFRSISFGKKDKQKPAKSEEATSPTSGTTSPTTAEAEAAPAGDAAVAEPSVATNGEAEKPAETATATSEPASKDEKPAENGSATEQEKQANGETEKAAPAPSTVEEAAKPKPAEEPATVTATESNTTATEEVPVKESQPEPEVVTNGHGAGEALTNGSSNGLAESPVTETAPVADNIPSNVDDEPPHQNGTNGTTTPPPTPVATEIEKGQQIEVDLAKDLKEKNAAAADVTTQEQLPVTCE

Target ORF information:

RefSeq Version	NM_164820.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster A kinase anchor protein 200 (Akap200), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164820.2

ORF Insert Sequence:

ATGGGTAAAG CTCAGAGCAA GCGTTCAATC GACATCACCA CCGATCCCAA GAAGGTCGGA 
GAGGGCGATG AGGTCGCCGG CAAGGTGGAG AAAATCGATG TGGACCAGAA GACGGACGCG 
CCGGCTGTGA ACGGAGACGC TGCCACGCCC AAAGAGGGTG GTGATGAAGC GGCGGCGGTA 
GAGAAAAAGG AAACTGAGGA GCATTCTGAG AACGACAAAG ATCTGACCAC CGAAAAGAGT 
GCCGCAGTCG CTGAGGGTGG TGATGCGGTC GCTGAAACGG CCAAGGGCGA GGAGGGATCT 
CCCAAGGAGG CTGCTGCTGG TGAGGATATA ACCCCGCTGG CAGACGAAAG CATCAAGTCC 
AAGTCCAAGA AGGACAAGGT CAAGAAGAAG TGGTCCTTCC GAAGCATTTC CTTTGGCAAG 
AAGGACAAAC AGAAACCGGC CAAGTCGGAG GAGGCTACGT CGCCCACCTC TGGCACAACG 
TCGCCCACGA CGGCCGAGGC AGAGGCTGCT CCAGCTGGGG ATGCAGCTGT CGCAGAACCA 
TCGGTGGCTA CAAATGGCGA GGCTGAAAAG CCAGCTGAGA CTGCCACTGC CACCAGCGAG 
CCGGCGTCCA AGGATGAGAA GCCCGCCGAA AATGGATCAG CGACCGAGCA GGAGAAACAG 
GCCAACGGAG AAACCGAAAA GGCGGCGCCA GCACCTTCCA CAGTTGAGGA AGCGGCGAAG 
CCCAAGCCCG CCGAAGAACC AGCCACAGTC ACTGCCACCG AATCGAACAC CACTGCCACC 
GAAGAAGTTC CCGTGAAAGA GAGCCAGCCA GAGCCCGAAG TGGTGACCAA TGGACATGGA 
GCCGGAGAGG CGCTGACCAA CGGATCAAGC AATGGACTGG CCGAGTCCCC AGTGACCGAG 
ACAGCACCTG TCGCTGACAA CATTCCGAGC AACGTTGATG ACGAGCCACC GCACCAGAAT 
GGCACCAATG GCACCACCAC GCCCCCGCCC ACTCCCGTGG CCACCGAGAT CGAGAAGGGA 
CAGCAAATTG AGGTGGATCT CGCCAAGGAT CTGAAGGAGA AGAATGCCGC CGCGGCAGAC 
GTTACAACAC AGGAACAACT GCCCGTTACA TGCGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Akap200 ( NM_001298823.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285752.1 Drosophila melanogaster A kinase anchor protein 200 (Akap200), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa16479
Clone ID Related Accession (Same CDS sequence)	NM_164820.2 , NM_001298823.1 , NM_058111.4
Accession Version	NM_001298823.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	A kinase anchor protein 200, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTAAAG CTCAGAGCAA GCGTTCAATC GACATCACCA CCGATCCCAA GAAGGTCGGA 
GAGGGCGATG AGGTCGCCGG CAAGGTGGAG AAAATCGATG TGGACCAGAA GACGGACGCG 
CCGGCTGTGA ACGGAGACGC TGCCACGCCC AAAGAGGGTG GTGATGAAGC GGCGGCGGTA 
GAGAAAAAGG AAACTGAGGA GCATTCTGAG AACGACAAAG ATCTGACCAC CGAAAAGAGT 
GCCGCAGTCG CTGAGGGTGG TGATGCGGTC GCTGAAACGG CCAAGGGCGA GGAGGGATCT 
CCCAAGGAGG CTGCTGCTGG TGAGGATATA ACCCCGCTGG CAGACGAAAG CATCAAGTCC 
AAGTCCAAGA AGGACAAGGT CAAGAAGAAG TGGTCCTTCC GAAGCATTTC CTTTGGCAAG 
AAGGACAAAC AGAAACCGGC CAAGTCGGAG GAGGCTACGT CGCCCACCTC TGGCACAACG 
TCGCCCACGA CGGCCGAGGC AGAGGCTGCT CCAGCTGGGG ATGCAGCTGT CGCAGAACCA 
TCGGTGGCTA CAAATGGCGA GGCTGAAAAG CCAGCTGAGA CTGCCACTGC CACCAGCGAG 
CCGGCGTCCA AGGATGAGAA GCCCGCCGAA AATGGATCAG CGACCGAGCA GGAGAAACAG 
GCCAACGGAG AAACCGAAAA GGCGGCGCCA GCACCTTCCA CAGTTGAGGA AGCGGCGAAG 
CCCAAGCCCG CCGAAGAACC AGCCACAGTC ACTGCCACCG AATCGAACAC CACTGCCACC 
GAAGAAGTTC CCGTGAAAGA GAGCCAGCCA GAGCCCGAAG TGGTGACCAA TGGACATGGA 
GCCGGAGAGG CGCTGACCAA CGGATCAAGC AATGGACTGG CCGAGTCCCC AGTGACCGAG 
ACAGCACCTG TCGCTGACAA CATTCCGAGC AACGTTGATG ACGAGCCACC GCACCAGAAT 
GGCACCAATG GCACCACCAC GCCCCCGCCC ACTCCCGTGG CCACCGAGAT CGAGAAGGGA 
CAGCAAATTG AGGTGGATCT CGCCAAGGAT CTGAAGGAGA AGAATGCCGC CGCGGCAGAC 
GTTACAACAC AGGAACAACT GCCCGTTACA TGCGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285752.1
CDS	182..1300
Translation	MGKAQSKRSIDITTDPKKVGEGDEVAGKVEKIDVDQKTDAPAVNGDAATPKEGGDEAAAVEKKETEEHSENDKDLTTEKSAAVAEGGDAVAETAKGEEGSPKEAAAGEDITPLADESIKSKSKKDKVKKKWSFRSISFGKKDKQKPAKSEEATSPTSGTTSPTTAEAEAAPAGDAAVAEPSVATNGEAEKPAETATATSEPASKDEKPAENGSATEQEKQANGETEKAAPAPSTVEEAAKPKPAEEPATVTATESNTTATEEVPVKESQPEPEVVTNGHGAGEALTNGSSNGLAESPVTETAPVADNIPSNVDDEPPHQNGTNGTTTPPPTPVATEIEKGQQIEVDLAKDLKEKNAAAADVTTQEQLPVTCE

Target ORF information:

RefSeq Version	NM_001298823.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster A kinase anchor protein 200 (Akap200), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298823.1

ORF Insert Sequence:

ATGGGTAAAG CTCAGAGCAA GCGTTCAATC GACATCACCA CCGATCCCAA GAAGGTCGGA 
GAGGGCGATG AGGTCGCCGG CAAGGTGGAG AAAATCGATG TGGACCAGAA GACGGACGCG 
CCGGCTGTGA ACGGAGACGC TGCCACGCCC AAAGAGGGTG GTGATGAAGC GGCGGCGGTA 
GAGAAAAAGG AAACTGAGGA GCATTCTGAG AACGACAAAG ATCTGACCAC CGAAAAGAGT 
GCCGCAGTCG CTGAGGGTGG TGATGCGGTC GCTGAAACGG CCAAGGGCGA GGAGGGATCT 
CCCAAGGAGG CTGCTGCTGG TGAGGATATA ACCCCGCTGG CAGACGAAAG CATCAAGTCC 
AAGTCCAAGA AGGACAAGGT CAAGAAGAAG TGGTCCTTCC GAAGCATTTC CTTTGGCAAG 
AAGGACAAAC AGAAACCGGC CAAGTCGGAG GAGGCTACGT CGCCCACCTC TGGCACAACG 
TCGCCCACGA CGGCCGAGGC AGAGGCTGCT CCAGCTGGGG ATGCAGCTGT CGCAGAACCA 
TCGGTGGCTA CAAATGGCGA GGCTGAAAAG CCAGCTGAGA CTGCCACTGC CACCAGCGAG 
CCGGCGTCCA AGGATGAGAA GCCCGCCGAA AATGGATCAG CGACCGAGCA GGAGAAACAG 
GCCAACGGAG AAACCGAAAA GGCGGCGCCA GCACCTTCCA CAGTTGAGGA AGCGGCGAAG 
CCCAAGCCCG CCGAAGAACC AGCCACAGTC ACTGCCACCG AATCGAACAC CACTGCCACC 
GAAGAAGTTC CCGTGAAAGA GAGCCAGCCA GAGCCCGAAG TGGTGACCAA TGGACATGGA 
GCCGGAGAGG CGCTGACCAA CGGATCAAGC AATGGACTGG CCGAGTCCCC AGTGACCGAG 
ACAGCACCTG TCGCTGACAA CATTCCGAGC AACGTTGATG ACGAGCCACC GCACCAGAAT 
GGCACCAATG GCACCACCAC GCCCCCGCCC ACTCCCGTGG CCACCGAGAT CGAGAAGGGA 
CAGCAAATTG AGGTGGATCT CGCCAAGGAT CTGAAGGAGA AGAATGCCGC CGCGGCAGAC 
GTTACAACAC AGGAACAACT GCCCGTTACA TGCGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Akap200 ( NM_058111.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477459.1 Drosophila melanogaster A kinase anchor protein 200 (Akap200), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa16479
Clone ID Related Accession (Same CDS sequence)	NM_164820.2 , NM_001298823.1 , NM_058111.4
Accession Version	NM_058111.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	A kinase anchor protein 200, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_058111.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTAAAG CTCAGAGCAA GCGTTCAATC GACATCACCA CCGATCCCAA GAAGGTCGGA 
GAGGGCGATG AGGTCGCCGG CAAGGTGGAG AAAATCGATG TGGACCAGAA GACGGACGCG 
CCGGCTGTGA ACGGAGACGC TGCCACGCCC AAAGAGGGTG GTGATGAAGC GGCGGCGGTA 
GAGAAAAAGG AAACTGAGGA GCATTCTGAG AACGACAAAG ATCTGACCAC CGAAAAGAGT 
GCCGCAGTCG CTGAGGGTGG TGATGCGGTC GCTGAAACGG CCAAGGGCGA GGAGGGATCT 
CCCAAGGAGG CTGCTGCTGG TGAGGATATA ACCCCGCTGG CAGACGAAAG CATCAAGTCC 
AAGTCCAAGA AGGACAAGGT CAAGAAGAAG TGGTCCTTCC GAAGCATTTC CTTTGGCAAG 
AAGGACAAAC AGAAACCGGC CAAGTCGGAG GAGGCTACGT CGCCCACCTC TGGCACAACG 
TCGCCCACGA CGGCCGAGGC AGAGGCTGCT CCAGCTGGGG ATGCAGCTGT CGCAGAACCA 
TCGGTGGCTA CAAATGGCGA GGCTGAAAAG CCAGCTGAGA CTGCCACTGC CACCAGCGAG 
CCGGCGTCCA AGGATGAGAA GCCCGCCGAA AATGGATCAG CGACCGAGCA GGAGAAACAG 
GCCAACGGAG AAACCGAAAA GGCGGCGCCA GCACCTTCCA CAGTTGAGGA AGCGGCGAAG 
CCCAAGCCCG CCGAAGAACC AGCCACAGTC ACTGCCACCG AATCGAACAC CACTGCCACC 
GAAGAAGTTC CCGTGAAAGA GAGCCAGCCA GAGCCCGAAG TGGTGACCAA TGGACATGGA 
GCCGGAGAGG CGCTGACCAA CGGATCAAGC AATGGACTGG CCGAGTCCCC AGTGACCGAG 
ACAGCACCTG TCGCTGACAA CATTCCGAGC AACGTTGATG ACGAGCCACC GCACCAGAAT 
GGCACCAATG GCACCACCAC GCCCCCGCCC ACTCCCGTGG CCACCGAGAT CGAGAAGGGA 
CAGCAAATTG AGGTGGATCT CGCCAAGGAT CTGAAGGAGA AGAATGCCGC CGCGGCAGAC 
GTTACAACAC AGGAACAACT GCCCGTTACA TGCGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477459.1
CDS	139..1257
Translation	MGKAQSKRSIDITTDPKKVGEGDEVAGKVEKIDVDQKTDAPAVNGDAATPKEGGDEAAAVEKKETEEHSENDKDLTTEKSAAVAEGGDAVAETAKGEEGSPKEAAAGEDITPLADESIKSKSKKDKVKKKWSFRSISFGKKDKQKPAKSEEATSPTSGTTSPTTAEAEAAPAGDAAVAEPSVATNGEAEKPAETATATSEPASKDEKPAENGSATEQEKQANGETEKAAPAPSTVEEAAKPKPAEEPATVTATESNTTATEEVPVKESQPEPEVVTNGHGAGEALTNGSSNGLAESPVTETAPVADNIPSNVDDEPPHQNGTNGTTTPPPTPVATEIEKGQQIEVDLAKDLKEKNAAAADVTTQEQLPVTCE

Target ORF information:

RefSeq Version	NM_058111.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster A kinase anchor protein 200 (Akap200), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058111.4

ORF Insert Sequence:

ATGGGTAAAG CTCAGAGCAA GCGTTCAATC GACATCACCA CCGATCCCAA GAAGGTCGGA 
GAGGGCGATG AGGTCGCCGG CAAGGTGGAG AAAATCGATG TGGACCAGAA GACGGACGCG 
CCGGCTGTGA ACGGAGACGC TGCCACGCCC AAAGAGGGTG GTGATGAAGC GGCGGCGGTA 
GAGAAAAAGG AAACTGAGGA GCATTCTGAG AACGACAAAG ATCTGACCAC CGAAAAGAGT 
GCCGCAGTCG CTGAGGGTGG TGATGCGGTC GCTGAAACGG CCAAGGGCGA GGAGGGATCT 
CCCAAGGAGG CTGCTGCTGG TGAGGATATA ACCCCGCTGG CAGACGAAAG CATCAAGTCC 
AAGTCCAAGA AGGACAAGGT CAAGAAGAAG TGGTCCTTCC GAAGCATTTC CTTTGGCAAG 
AAGGACAAAC AGAAACCGGC CAAGTCGGAG GAGGCTACGT CGCCCACCTC TGGCACAACG 
TCGCCCACGA CGGCCGAGGC AGAGGCTGCT CCAGCTGGGG ATGCAGCTGT CGCAGAACCA 
TCGGTGGCTA CAAATGGCGA GGCTGAAAAG CCAGCTGAGA CTGCCACTGC CACCAGCGAG 
CCGGCGTCCA AGGATGAGAA GCCCGCCGAA AATGGATCAG CGACCGAGCA GGAGAAACAG 
GCCAACGGAG AAACCGAAAA GGCGGCGCCA GCACCTTCCA CAGTTGAGGA AGCGGCGAAG 
CCCAAGCCCG CCGAAGAACC AGCCACAGTC ACTGCCACCG AATCGAACAC CACTGCCACC 
GAAGAAGTTC CCGTGAAAGA GAGCCAGCCA GAGCCCGAAG TGGTGACCAA TGGACATGGA 
GCCGGAGAGG CGCTGACCAA CGGATCAAGC AATGGACTGG CCGAGTCCCC AGTGACCGAG 
ACAGCACCTG TCGCTGACAA CATTCCGAGC AACGTTGATG ACGAGCCACC GCACCAGAAT 
GGCACCAATG GCACCACCAC GCCCCCGCCC ACTCCCGTGG CCACCGAGAT CGAGAAGGGA 
CAGCAAATTG AGGTGGATCT CGCCAAGGAT CTGAAGGAGA AGAATGCCGC CGCGGCAGAC 
GTTACAACAC AGGAACAACT GCCCGTTACA TGCGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Akap200 ( NM_058112.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477460.1 Drosophila melanogaster A kinase anchor protein 200 (Akap200), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa16477
Clone ID Related Accession (Same CDS sequence)	NM_058112.4 , NM_001298822.1
Accession Version	NM_058112.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	A kinase anchor protein 200, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_058112.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTAAAG CTCAGAGCAA GCGTTCAATC GACATCACCA CCGATCCCAA GAAGGTCGGA 
GAGGGCGATG AGGTCGCCGG CAAGGTGGAG AAAATCGATG TGGACCAGAA GACGGACGCG 
CCGGCTGTGA ACGGAGACGC TGCCACGCCC AAAGAGGGTG GTGATGAAGC GGCGGCGGTA 
GAGAAAAAGG AAACTGAGGA GCATTCTGAG AACGACAAAG ATCTGACCAC CGAAAAGAGT 
GCCGCAGTCG CTGAGGGTGG TGATGCGGTC GCTGAAACGG CCAAGGGCGA GGAGGGATCT 
CCCAAGGAGG CTGCTGCTGG TGAGGATATA ACCCCGCTGG CAGACGAAAG CATCAAGTCC 
AAGTCCAAGA AGGACAAGGT CAAGAAGAAG TGGTCCTTCC GAAGCATTTC CTTTGGCAAG 
AAGGACAAAC AGAAACCGGC CAAGTCGGAG GAGGCTACGT CGCCCACCTC TGGCACAACG 
TCGCCCACGA CGGCCGAGGC AGAGGCTGCT CCAGCTGGGG ATGCAGCTGT CGCAGAACCA 
TCGGTGGCTA CAAATGGCGA GGCTGAAAAG CCAGCTGAGA CTGCCACTGC CACCAGCGAG 
CCGGCGTCCA AGGATGAGAA GCCCGCCGAA AATGGATCAG CGACCGAGCA GGAGAAACAG 
GCCAACGGAG AAACCGAAAA GGCGGCGCCA GCACCTTCCA CAGTTGAGGA AGCGGCGAAG 
CCCAAGCCCG CCGAAGAACC AGCCACAGTC ACTGCCACCG AATCGAACAC CACTGCCACC 
GAAGAAGTTC CCGTGAAAGA GAGCCAGCCA GAGCCCGAAG TGGTGACCAA TGGACATGGA 
GCCGGAGAGG CGCTGACCAA CGGATCAAGC AATGGACTGG CCGAGTCCCC AGTGACCGAG 
ACAGCACCTG TCGCTGACAA CATTCCGAGC AACGTTGATG ACGAGCCACC GCACCAGAAT 
GGCACCAATG GCACCACCAC GCCCCCGCCC ACTCCCGTGG CCACCGAGAT CGAGAAGGGA 
CAGCAAATTG AGGCCAGCAG TGAAGTGATT GAAACAGTGA CCCCCAGCCA AGCGGAAGAG 
GAAGTTGTGG CAGCAATCAT CAAGGCGGTT AGCAGCGAAC CTGAAGCCGA AACGGAGACT 
GAGACAGAGG CCGAGGGATT CGTGCTTGTT GCTCCTGTGT CCACCGAAGT CGAAGTTCCC 
GTTTCCATTT CCCCCATTGA ACCCGTGGCA GAGGTTTCCC AAGTTAAAAT TGAACCACTT 
GTCGAAATTC CCGAAGTTGA AGCCAAGTCC GTAGCCGATG TGTCTGAAGC TGATACGGAA 
TCCGTACCAA AAGTTTCTGA ATTGAAAACT GAAGTTTCCG AAATTGAGTT CGAGTCCGTT 
ATTGTGGAGA CGAGAAGCAG CAGTCCGCCA CCTCCATTGC CCAAATCCCC GCCTCCGTCT 
CGTGTCTCCG CATTTGTGCT GTCCGAGGAT GTTATCGAGG AGCAGGTTAC CCCGAATGTC 
CCCGAAGTAA ACGACGTGAA GCCCGATGAG ATCGAACAGC AGGCAATCAG CATAGTGGCT 
GAAATCACCG AACAGGCAGC AGAAATTGTC ACCGAACAAG AGAAACAACA GGAGGAGGCC 
AAGGTGGATT CTGTTCCTGA AACAATTGAG GAATCCAGCT CCACGGTTGT GGTTGAGGAA 
GTTTTACCAG TTCAAAACGA TGAAGTAACG GCTCCTTCTC CTACTCCTGA TGATGTCCAG 
AAACCTATTG AAGATCAAGA TACACCCGAC GAAAAAGAGT CCTACCCAGT TCCAGATCCC 
ATTGACCCGG CGGCCGTAAA CGATGAGGTC GCTGTTACAG AAGCTGTAGA TTGTGAAGTT 
GAGAAAGAAA CCGGTAGCAT CAGCTCCAAC GTTGCTGAAT CTTCTTCAGT ATCCGACGAG 
CAGGCGGCAA TCGAAAATCA GGTTGAAATC CTCGAAGAAC AGACAGTAGC TGTTGAGGAA 
ACCACCGAAC AAGAGACATC AGACCAGCAA GTTATTTCCG AGGAGGCGCA TAGCGATAAC 
GATAAGGAGA ATGAAATCGA CCTGGTTGAA AACATCATTA GCGATCTGGA TGCTCCGATT 
ACCAAAGCAG GCGGCGATCT GCTGGTAGAA TTGGATGCGA GATCCGCGGA ACAGGAGGGC 
GAGAGCAACA ACAAGGTGGA TCTCGCCAAG GATCTGAAGG AGAAGAATGC CGCCGCGGCA 
GACGTTACAA CACAGGAACA ACTGCCCGTT ACATGCGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477460.1
CDS	182..2443
Translation	MGKAQSKRSIDITTDPKKVGEGDEVAGKVEKIDVDQKTDAPAVNGDAATPKEGGDEAAAVEKKETEEHSENDKDLTTEKSAAVAEGGDAVAETAKGEEGSPKEAAAGEDITPLADESIKSKSKKDKVKKKWSFRSISFGKKDKQKPAKSEEATSPTSGTTSPTTAEAEAAPAGDAAVAEPSVATNGEAEKPAETATATSEPASKDEKPAENGSATEQEKQANGETEKAAPAPSTVEEAAKPKPAEEPATVTATESNTTATEEVPVKESQPEPEVVTNGHGAGEALTNGSSNGLAESPVTETAPVADNIPSNVDDEPPHQNGTNGTTTPPPTPVATEIEKGQQIEASSEVIETVTPSQAEEEVVAAIIKAVSSEPEAETETETEAEGFVLVAPVSTEVEVPVSISPIEPVAEVSQVKIEPLVEIPEVEAKSVADVSEADTESVPKVSELKTEVSEIEFESVIVETRSSSPPPPLPKSPPPSRVSAFVLSEDVIEEQVTPNVPEVNDVKPDEIEQQAISIVAEITEQAAEIVTEQEKQQEEAKVDSVPETIEESSSTVVVEEVLPVQNDEVTAPSPTPDDVQKPIEDQDTPDEKESYPVPDPIDPAAVNDEVAVTEAVDCEVEKETGSISSNVAESSSVSDEQAAIENQVEILEEQTVAVEETTEQETSDQQVISEEAHSDNDKENEIDLVENIISDLDAPITKAGGDLLVELDARSAEQEGESNNKVDLAKDLKEKNAAAADVTTQEQLPVTCE

Target ORF information:

RefSeq Version	NM_058112.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster A kinase anchor protein 200 (Akap200), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058112.4

ORF Insert Sequence:

ATGGGTAAAG CTCAGAGCAA GCGTTCAATC GACATCACCA CCGATCCCAA GAAGGTCGGA 
GAGGGCGATG AGGTCGCCGG CAAGGTGGAG AAAATCGATG TGGACCAGAA GACGGACGCG 
CCGGCTGTGA ACGGAGACGC TGCCACGCCC AAAGAGGGTG GTGATGAAGC GGCGGCGGTA 
GAGAAAAAGG AAACTGAGGA GCATTCTGAG AACGACAAAG ATCTGACCAC CGAAAAGAGT 
GCCGCAGTCG CTGAGGGTGG TGATGCGGTC GCTGAAACGG CCAAGGGCGA GGAGGGATCT 
CCCAAGGAGG CTGCTGCTGG TGAGGATATA ACCCCGCTGG CAGACGAAAG CATCAAGTCC 
AAGTCCAAGA AGGACAAGGT CAAGAAGAAG TGGTCCTTCC GAAGCATTTC CTTTGGCAAG 
AAGGACAAAC AGAAACCGGC CAAGTCGGAG GAGGCTACGT CGCCCACCTC TGGCACAACG 
TCGCCCACGA CGGCCGAGGC AGAGGCTGCT CCAGCTGGGG ATGCAGCTGT CGCAGAACCA 
TCGGTGGCTA CAAATGGCGA GGCTGAAAAG CCAGCTGAGA CTGCCACTGC CACCAGCGAG 
CCGGCGTCCA AGGATGAGAA GCCCGCCGAA AATGGATCAG CGACCGAGCA GGAGAAACAG 
GCCAACGGAG AAACCGAAAA GGCGGCGCCA GCACCTTCCA CAGTTGAGGA AGCGGCGAAG 
CCCAAGCCCG CCGAAGAACC AGCCACAGTC ACTGCCACCG AATCGAACAC CACTGCCACC 
GAAGAAGTTC CCGTGAAAGA GAGCCAGCCA GAGCCCGAAG TGGTGACCAA TGGACATGGA 
GCCGGAGAGG CGCTGACCAA CGGATCAAGC AATGGACTGG CCGAGTCCCC AGTGACCGAG 
ACAGCACCTG TCGCTGACAA CATTCCGAGC AACGTTGATG ACGAGCCACC GCACCAGAAT 
GGCACCAATG GCACCACCAC GCCCCCGCCC ACTCCCGTGG CCACCGAGAT CGAGAAGGGA 
CAGCAAATTG AGGCCAGCAG TGAAGTGATT GAAACAGTGA CCCCCAGCCA AGCGGAAGAG 
GAAGTTGTGG CAGCAATCAT CAAGGCGGTT AGCAGCGAAC CTGAAGCCGA AACGGAGACT 
GAGACAGAGG CCGAGGGATT CGTGCTTGTT GCTCCTGTGT CCACCGAAGT CGAAGTTCCC 
GTTTCCATTT CCCCCATTGA ACCCGTGGCA GAGGTTTCCC AAGTTAAAAT TGAACCACTT 
GTCGAAATTC CCGAAGTTGA AGCCAAGTCC GTAGCCGATG TGTCTGAAGC TGATACGGAA 
TCCGTACCAA AAGTTTCTGA ATTGAAAACT GAAGTTTCCG AAATTGAGTT CGAGTCCGTT 
ATTGTGGAGA CGAGAAGCAG CAGTCCGCCA CCTCCATTGC CCAAATCCCC GCCTCCGTCT 
CGTGTCTCCG CATTTGTGCT GTCCGAGGAT GTTATCGAGG AGCAGGTTAC CCCGAATGTC 
CCCGAAGTAA ACGACGTGAA GCCCGATGAG ATCGAACAGC AGGCAATCAG CATAGTGGCT 
GAAATCACCG AACAGGCAGC AGAAATTGTC ACCGAACAAG AGAAACAACA GGAGGAGGCC 
AAGGTGGATT CTGTTCCTGA AACAATTGAG GAATCCAGCT CCACGGTTGT GGTTGAGGAA 
GTTTTACCAG TTCAAAACGA TGAAGTAACG GCTCCTTCTC CTACTCCTGA TGATGTCCAG 
AAACCTATTG AAGATCAAGA TACACCCGAC GAAAAAGAGT CCTACCCAGT TCCAGATCCC 
ATTGACCCGG CGGCCGTAAA CGATGAGGTC GCTGTTACAG AAGCTGTAGA TTGTGAAGTT 
GAGAAAGAAA CCGGTAGCAT CAGCTCCAAC GTTGCTGAAT CTTCTTCAGT ATCCGACGAG 
CAGGCGGCAA TCGAAAATCA GGTTGAAATC CTCGAAGAAC AGACAGTAGC TGTTGAGGAA 
ACCACCGAAC AAGAGACATC AGACCAGCAA GTTATTTCCG AGGAGGCGCA TAGCGATAAC 
GATAAGGAGA ATGAAATCGA CCTGGTTGAA AACATCATTA GCGATCTGGA TGCTCCGATT 
ACCAAAGCAG GCGGCGATCT GCTGGTAGAA TTGGATGCGA GATCCGCGGA ACAGGAGGGC 
GAGAGCAACA ACAAGGTGGA TCTCGCCAAG GATCTGAAGG AGAAGAATGC CGCCGCGGCA 
GACGTTACAA CACAGGAACA ACTGCCCGTT ACATGCGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Akap200 ( NM_001298822.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285751.1 Drosophila melanogaster A kinase anchor protein 200 (Akap200), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa16477
Clone ID Related Accession (Same CDS sequence)	NM_058112.4 , NM_001298822.1
Accession Version	NM_001298822.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	A kinase anchor protein 200, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTAAAG CTCAGAGCAA GCGTTCAATC GACATCACCA CCGATCCCAA GAAGGTCGGA 
GAGGGCGATG AGGTCGCCGG CAAGGTGGAG AAAATCGATG TGGACCAGAA GACGGACGCG 
CCGGCTGTGA ACGGAGACGC TGCCACGCCC AAAGAGGGTG GTGATGAAGC GGCGGCGGTA 
GAGAAAAAGG AAACTGAGGA GCATTCTGAG AACGACAAAG ATCTGACCAC CGAAAAGAGT 
GCCGCAGTCG CTGAGGGTGG TGATGCGGTC GCTGAAACGG CCAAGGGCGA GGAGGGATCT 
CCCAAGGAGG CTGCTGCTGG TGAGGATATA ACCCCGCTGG CAGACGAAAG CATCAAGTCC 
AAGTCCAAGA AGGACAAGGT CAAGAAGAAG TGGTCCTTCC GAAGCATTTC CTTTGGCAAG 
AAGGACAAAC AGAAACCGGC CAAGTCGGAG GAGGCTACGT CGCCCACCTC TGGCACAACG 
TCGCCCACGA CGGCCGAGGC AGAGGCTGCT CCAGCTGGGG ATGCAGCTGT CGCAGAACCA 
TCGGTGGCTA CAAATGGCGA GGCTGAAAAG CCAGCTGAGA CTGCCACTGC CACCAGCGAG 
CCGGCGTCCA AGGATGAGAA GCCCGCCGAA AATGGATCAG CGACCGAGCA GGAGAAACAG 
GCCAACGGAG AAACCGAAAA GGCGGCGCCA GCACCTTCCA CAGTTGAGGA AGCGGCGAAG 
CCCAAGCCCG CCGAAGAACC AGCCACAGTC ACTGCCACCG AATCGAACAC CACTGCCACC 
GAAGAAGTTC CCGTGAAAGA GAGCCAGCCA GAGCCCGAAG TGGTGACCAA TGGACATGGA 
GCCGGAGAGG CGCTGACCAA CGGATCAAGC AATGGACTGG CCGAGTCCCC AGTGACCGAG 
ACAGCACCTG TCGCTGACAA CATTCCGAGC AACGTTGATG ACGAGCCACC GCACCAGAAT 
GGCACCAATG GCACCACCAC GCCCCCGCCC ACTCCCGTGG CCACCGAGAT CGAGAAGGGA 
CAGCAAATTG AGGCCAGCAG TGAAGTGATT GAAACAGTGA CCCCCAGCCA AGCGGAAGAG 
GAAGTTGTGG CAGCAATCAT CAAGGCGGTT AGCAGCGAAC CTGAAGCCGA AACGGAGACT 
GAGACAGAGG CCGAGGGATT CGTGCTTGTT GCTCCTGTGT CCACCGAAGT CGAAGTTCCC 
GTTTCCATTT CCCCCATTGA ACCCGTGGCA GAGGTTTCCC AAGTTAAAAT TGAACCACTT 
GTCGAAATTC CCGAAGTTGA AGCCAAGTCC GTAGCCGATG TGTCTGAAGC TGATACGGAA 
TCCGTACCAA AAGTTTCTGA ATTGAAAACT GAAGTTTCCG AAATTGAGTT CGAGTCCGTT 
ATTGTGGAGA CGAGAAGCAG CAGTCCGCCA CCTCCATTGC CCAAATCCCC GCCTCCGTCT 
CGTGTCTCCG CATTTGTGCT GTCCGAGGAT GTTATCGAGG AGCAGGTTAC CCCGAATGTC 
CCCGAAGTAA ACGACGTGAA GCCCGATGAG ATCGAACAGC AGGCAATCAG CATAGTGGCT 
GAAATCACCG AACAGGCAGC AGAAATTGTC ACCGAACAAG AGAAACAACA GGAGGAGGCC 
AAGGTGGATT CTGTTCCTGA AACAATTGAG GAATCCAGCT CCACGGTTGT GGTTGAGGAA 
GTTTTACCAG TTCAAAACGA TGAAGTAACG GCTCCTTCTC CTACTCCTGA TGATGTCCAG 
AAACCTATTG AAGATCAAGA TACACCCGAC GAAAAAGAGT CCTACCCAGT TCCAGATCCC 
ATTGACCCGG CGGCCGTAAA CGATGAGGTC GCTGTTACAG AAGCTGTAGA TTGTGAAGTT 
GAGAAAGAAA CCGGTAGCAT CAGCTCCAAC GTTGCTGAAT CTTCTTCAGT ATCCGACGAG 
CAGGCGGCAA TCGAAAATCA GGTTGAAATC CTCGAAGAAC AGACAGTAGC TGTTGAGGAA 
ACCACCGAAC AAGAGACATC AGACCAGCAA GTTATTTCCG AGGAGGCGCA TAGCGATAAC 
GATAAGGAGA ATGAAATCGA CCTGGTTGAA AACATCATTA GCGATCTGGA TGCTCCGATT 
ACCAAAGCAG GCGGCGATCT GCTGGTAGAA TTGGATGCGA GATCCGCGGA ACAGGAGGGC 
GAGAGCAACA ACAAGGTGGA TCTCGCCAAG GATCTGAAGG AGAAGAATGC CGCCGCGGCA 
GACGTTACAA CACAGGAACA ACTGCCCGTT ACATGCGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285751.1
CDS	182..2443
Translation	MGKAQSKRSIDITTDPKKVGEGDEVAGKVEKIDVDQKTDAPAVNGDAATPKEGGDEAAAVEKKETEEHSENDKDLTTEKSAAVAEGGDAVAETAKGEEGSPKEAAAGEDITPLADESIKSKSKKDKVKKKWSFRSISFGKKDKQKPAKSEEATSPTSGTTSPTTAEAEAAPAGDAAVAEPSVATNGEAEKPAETATATSEPASKDEKPAENGSATEQEKQANGETEKAAPAPSTVEEAAKPKPAEEPATVTATESNTTATEEVPVKESQPEPEVVTNGHGAGEALTNGSSNGLAESPVTETAPVADNIPSNVDDEPPHQNGTNGTTTPPPTPVATEIEKGQQIEASSEVIETVTPSQAEEEVVAAIIKAVSSEPEAETETETEAEGFVLVAPVSTEVEVPVSISPIEPVAEVSQVKIEPLVEIPEVEAKSVADVSEADTESVPKVSELKTEVSEIEFESVIVETRSSSPPPPLPKSPPPSRVSAFVLSEDVIEEQVTPNVPEVNDVKPDEIEQQAISIVAEITEQAAEIVTEQEKQQEEAKVDSVPETIEESSSTVVVEEVLPVQNDEVTAPSPTPDDVQKPIEDQDTPDEKESYPVPDPIDPAAVNDEVAVTEAVDCEVEKETGSISSNVAESSSVSDEQAAIENQVEILEEQTVAVEETTEQETSDQQVISEEAHSDNDKENEIDLVENIISDLDAPITKAGGDLLVELDARSAEQEGESNNKVDLAKDLKEKNAAAADVTTQEQLPVTCE

Target ORF information:

RefSeq Version	NM_001298822.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster A kinase anchor protein 200 (Akap200), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298822.1

ORF Insert Sequence:

ATGGGTAAAG CTCAGAGCAA GCGTTCAATC GACATCACCA CCGATCCCAA GAAGGTCGGA 
GAGGGCGATG AGGTCGCCGG CAAGGTGGAG AAAATCGATG TGGACCAGAA GACGGACGCG 
CCGGCTGTGA ACGGAGACGC TGCCACGCCC AAAGAGGGTG GTGATGAAGC GGCGGCGGTA 
GAGAAAAAGG AAACTGAGGA GCATTCTGAG AACGACAAAG ATCTGACCAC CGAAAAGAGT 
GCCGCAGTCG CTGAGGGTGG TGATGCGGTC GCTGAAACGG CCAAGGGCGA GGAGGGATCT 
CCCAAGGAGG CTGCTGCTGG TGAGGATATA ACCCCGCTGG CAGACGAAAG CATCAAGTCC 
AAGTCCAAGA AGGACAAGGT CAAGAAGAAG TGGTCCTTCC GAAGCATTTC CTTTGGCAAG 
AAGGACAAAC AGAAACCGGC CAAGTCGGAG GAGGCTACGT CGCCCACCTC TGGCACAACG 
TCGCCCACGA CGGCCGAGGC AGAGGCTGCT CCAGCTGGGG ATGCAGCTGT CGCAGAACCA 
TCGGTGGCTA CAAATGGCGA GGCTGAAAAG CCAGCTGAGA CTGCCACTGC CACCAGCGAG 
CCGGCGTCCA AGGATGAGAA GCCCGCCGAA AATGGATCAG CGACCGAGCA GGAGAAACAG 
GCCAACGGAG AAACCGAAAA GGCGGCGCCA GCACCTTCCA CAGTTGAGGA AGCGGCGAAG 
CCCAAGCCCG CCGAAGAACC AGCCACAGTC ACTGCCACCG AATCGAACAC CACTGCCACC 
GAAGAAGTTC CCGTGAAAGA GAGCCAGCCA GAGCCCGAAG TGGTGACCAA TGGACATGGA 
GCCGGAGAGG CGCTGACCAA CGGATCAAGC AATGGACTGG CCGAGTCCCC AGTGACCGAG 
ACAGCACCTG TCGCTGACAA CATTCCGAGC AACGTTGATG ACGAGCCACC GCACCAGAAT 
GGCACCAATG GCACCACCAC GCCCCCGCCC ACTCCCGTGG CCACCGAGAT CGAGAAGGGA 
CAGCAAATTG AGGCCAGCAG TGAAGTGATT GAAACAGTGA CCCCCAGCCA AGCGGAAGAG 
GAAGTTGTGG CAGCAATCAT CAAGGCGGTT AGCAGCGAAC CTGAAGCCGA AACGGAGACT 
GAGACAGAGG CCGAGGGATT CGTGCTTGTT GCTCCTGTGT CCACCGAAGT CGAAGTTCCC 
GTTTCCATTT CCCCCATTGA ACCCGTGGCA GAGGTTTCCC AAGTTAAAAT TGAACCACTT 
GTCGAAATTC CCGAAGTTGA AGCCAAGTCC GTAGCCGATG TGTCTGAAGC TGATACGGAA 
TCCGTACCAA AAGTTTCTGA ATTGAAAACT GAAGTTTCCG AAATTGAGTT CGAGTCCGTT 
ATTGTGGAGA CGAGAAGCAG CAGTCCGCCA CCTCCATTGC CCAAATCCCC GCCTCCGTCT 
CGTGTCTCCG CATTTGTGCT GTCCGAGGAT GTTATCGAGG AGCAGGTTAC CCCGAATGTC 
CCCGAAGTAA ACGACGTGAA GCCCGATGAG ATCGAACAGC AGGCAATCAG CATAGTGGCT 
GAAATCACCG AACAGGCAGC AGAAATTGTC ACCGAACAAG AGAAACAACA GGAGGAGGCC 
AAGGTGGATT CTGTTCCTGA AACAATTGAG GAATCCAGCT CCACGGTTGT GGTTGAGGAA 
GTTTTACCAG TTCAAAACGA TGAAGTAACG GCTCCTTCTC CTACTCCTGA TGATGTCCAG 
AAACCTATTG AAGATCAAGA TACACCCGAC GAAAAAGAGT CCTACCCAGT TCCAGATCCC 
ATTGACCCGG CGGCCGTAAA CGATGAGGTC GCTGTTACAG AAGCTGTAGA TTGTGAAGTT 
GAGAAAGAAA CCGGTAGCAT CAGCTCCAAC GTTGCTGAAT CTTCTTCAGT ATCCGACGAG 
CAGGCGGCAA TCGAAAATCA GGTTGAAATC CTCGAAGAAC AGACAGTAGC TGTTGAGGAA 
ACCACCGAAC AAGAGACATC AGACCAGCAA GTTATTTCCG AGGAGGCGCA TAGCGATAAC 
GATAAGGAGA ATGAAATCGA CCTGGTTGAA AACATCATTA GCGATCTGGA TGCTCCGATT 
ACCAAAGCAG GCGGCGATCT GCTGGTAGAA TTGGATGCGA GATCCGCGGA ACAGGAGGGC 
GAGAGCAACA ACAAGGTGGA TCTCGCCAAG GATCTGAAGG AGAAGAATGC CGCCGCGGCA 
GACGTTACAA CACAGGAACA ACTGCCCGTT ACATGCGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Akap200 ( NM_175984.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_787998.1 Drosophila melanogaster A kinase anchor protein 200 (Akap200), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa16478
Clone ID Related Accession (Same CDS sequence)	NM_175984.1 , NM_001298824.1
Accession Version	NM_175984.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	A kinase anchor protein 200, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTAAAG CTCAGAGCAA GCGTTCAATC GACATCACCA CCGATCCCAA GAAGGTCGGA 
GAGGGCGATG AGGTCGCCGG CAAGGTGGAG AAAATCGATG TGGACCAGAA GACGGACGCG 
CCGGCTGTGA ACGGAGACGC TGCCACGCCC AAAGAGGGTG GTGATGAAGC GGCGGCGGTA 
GAGAAAAAGG AAACTGAGGA GCATTCTGAG AACGACAAAG ATCTGACCAC CGAAAAGAGT 
GCCGCAGTCG CTGAGGGTGG TGATGCGGTC GCTGAAACGG CCAAGGGCGA GGAGGGATCT 
CCCAAGGAGG CTGCTGCTGG TGAGGATATA ACCCCGCTGG CAGACGAAAG CATCAAGTCC 
AAGTCCAAGA AGGACAAGGT CAAGAAGAAG TGGTCCTTCC GAAGCATTTC CTTTGGCAAG 
AAGGACAAAC AGAAACCGGC CAAGTCGGAG GAGGCTACGT CGCCCACCTC TGGCACAACG 
TCGCCCACGA CGGCCGAGGC AGAGGCTGCT CCAGCTGGGG ATGCAGCTGT CGCAGAACCA 
TCGGTGGCTA CAAATGGCGA GGCTGAAAAG CCAGCTGAGA CTGCCACTGC CACCAGCGAG 
CCGGCGTCCA AGGATGAGAA GCCCGCCGAA AATGGATCAG CGACCGAGCA GGAGAAACAG 
GCCAACGGAG AAACCGAAAA GGCGGCGCCA GCACCTTCCA CAGTTGAGGA AGCGGCGAAG 
CCCAAGCCCG CCGAAGAACC AGCCACAGTC ACTGCCACCG AATCGAACAC CACTGCCACC 
GAAGAAGTTC CCGTGAAAGA GAGCCAGCCA GAGCCCGAAG TGGTGACCAA TGGACATGGA 
GCCGGAGAGG CGCTGACCAA CGGATCAAGC AATGGACTGG CCGAGTCCCC AGTGACCGAG 
ACAGCACCTG TCGCTGACAA CATTCCGAGC AACGTTGATG ACGAGCCACC GCACCAGAAT 
GGCACCAATG GCACCACCAC GCCCCCGCCC ACTCCCGTGG CCACCGAGAT CGAGAAGGGA 
CAGCAAATTG AGGTAAACAA CCACACAAGA ACAGACAACA CCACACAACA CAACAGCAAA 
AACATCACTG TAAATACAAG TACAACAACA ACAAAAACAA CCGAAATCAC AGAGAACACT 
GCACTAAAAA CAATAAGTAC AGATACAGAA AGAACTTGCA CAAGCACTGT GACCGAGACC 
GTTGTAGCAG CCGCAGAGTT AGTTCAGACC CAAACAATCC TAACCAAAAC AACAAACACG 
ATCCTTCCCA CCTCCCACAC GCAGGCCAGC AGTGAAGTGA TTGAAACAGT GACCCCCAGC 
CAAGCGGAAG AGGAAGTTGT GGCAGCAATC ATCAAGGCGG TTAGCAGCGA ACCTGAAGCC 
GAAACGGAGA CTGAGACAGA GGCCGAGGGA TTCGTGCTTG TTGCTCCTGT GTCCACCGAA 
GTCGAAGTTC CCGTTTCCAT TTCCCCCATT GAACCCGTGG CAGAGGTTTC CCAAGTTAAA 
ATTGAACCAC TTGTCGAAAT TCCCGAAGTT GAAGCCAAGT CCGTAGCCGA TGTGTCTGAA 
GCTGATACGG AATCCGTACC AAAAGTTTCT GAATTGAAAA CTGAAGTTTC CGAAATTGAG 
TTCGAGTCCG TTATTGTGGA GACGAGAAGC AGCAGTCCGC CACCTCCATT GCCCAAATCC 
CCGCCTCCGT CTCGTGTCTC CGCATTTGTG CTGTCCGAGG ATGTTATCGA GGAGCAGGTT 
ACCCCGAATG TCCCCGAAGT AAACGACGTG AAGCCCGATG AGATCGAACA GCAGGCAATC 
AGCATAGTGG CTGAAATCAC CGAACAGGCA GCAGAAATTG TCACCGAACA AGAGAAACAA 
CAGGAGGAGG CCAAGGTGGA TTCTGTTCCT GAAACAATTG AGGAATCCAG CTCCACGGTT 
GTGGTTGAGG AAGTTTTACC AGTTCAAAAC GATGAAGTAA CGGCTCCTTC TCCTACTCCT 
GATGATGTCC AGAAACCTAT TGAAGATCAA GATACACCCG ACGAAAAAGA GTCCTACCCA 
GTTCCAGATC CCATTGACCC GGCGGCCGTA AACGATGAGG TCGCTGTTAC AGAAGCTGTA 
GATTGTGAAG TTGAGAAAGA AACCGGTAGC ATCAGCTCCA ACGTTGCTGA ATCTTCTTCA 
GTATCCGACG AGCAGGCGGC AATCGAAAAT CAGGTTGAAA TCCTCGAAGA ACAGACAGTA 
GCTGTTGAGG AAACCACCGA ACAAGAGACA TCAGACCAGC AAGTTATTTC CGAGGAGGCG 
CATAGCGATA ACGATAAGGA GAATGAAATC GACCTGGTTG AAAACATCAT TAGCGATCTG 
GATGCTCCGA TTACCAAAGC AGGCGGCGAT CTGCTGGTAG AATTGGATGC GAGATCCGCG 
GAACAGGAGG GCGAGAGCAA CAACAAGGTG GATCTCGCCA AGGATCTGAA GGAGAAGAAT 
GCCGCCGCGG CAGACGTTAC AACACAGGAA CAACTGCCCG TTACATGCGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_787998.1
CDS	235..2748
Translation	MGKAQSKRSIDITTDPKKVGEGDEVAGKVEKIDVDQKTDAPAVNGDAATPKEGGDEAAAVEKKETEEHSENDKDLTTEKSAAVAEGGDAVAETAKGEEGSPKEAAAGEDITPLADESIKSKSKKDKVKKKWSFRSISFGKKDKQKPAKSEEATSPTSGTTSPTTAEAEAAPAGDAAVAEPSVATNGEAEKPAETATATSEPASKDEKPAENGSATEQEKQANGETEKAAPAPSTVEEAAKPKPAEEPATVTATESNTTATEEVPVKESQPEPEVVTNGHGAGEALTNGSSNGLAESPVTETAPVADNIPSNVDDEPPHQNGTNGTTTPPPTPVATEIEKGQQIEVNNHTRTDNTTQHNSKNITVNTSTTTTKTTEITENTALKTISTDTERTCTSTVTETVVAAAELVQTQTILTKTTNTILPTSHTQASSEVIETVTPSQAEEEVVAAIIKAVSSEPEAETETETEAEGFVLVAPVSTEVEVPVSISPIEPVAEVSQVKIEPLVEIPEVEAKSVADVSEADTESVPKVSELKTEVSEIEFESVIVETRSSSPPPPLPKSPPPSRVSAFVLSEDVIEEQVTPNVPEVNDVKPDEIEQQAISIVAEITEQAAEIVTEQEKQQEEAKVDSVPETIEESSSTVVVEEVLPVQNDEVTAPSPTPDDVQKPIEDQDTPDEKESYPVPDPIDPAAVNDEVAVTEAVDCEVEKETGSISSNVAESSSVSDEQAAIENQVEILEEQTVAVEETTEQETSDQQVISEEAHSDNDKENEIDLVENIISDLDAPITKAGGDLLVELDARSAEQEGESNNKVDLAKDLKEKNAAAADVTTQEQLPVTCE

Target ORF information:

RefSeq Version	NM_175984.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster A kinase anchor protein 200 (Akap200), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175984.1

ORF Insert Sequence:

ATGGGTAAAG CTCAGAGCAA GCGTTCAATC GACATCACCA CCGATCCCAA GAAGGTCGGA 
GAGGGCGATG AGGTCGCCGG CAAGGTGGAG AAAATCGATG TGGACCAGAA GACGGACGCG 
CCGGCTGTGA ACGGAGACGC TGCCACGCCC AAAGAGGGTG GTGATGAAGC GGCGGCGGTA 
GAGAAAAAGG AAACTGAGGA GCATTCTGAG AACGACAAAG ATCTGACCAC CGAAAAGAGT 
GCCGCAGTCG CTGAGGGTGG TGATGCGGTC GCTGAAACGG CCAAGGGCGA GGAGGGATCT 
CCCAAGGAGG CTGCTGCTGG TGAGGATATA ACCCCGCTGG CAGACGAAAG CATCAAGTCC 
AAGTCCAAGA AGGACAAGGT CAAGAAGAAG TGGTCCTTCC GAAGCATTTC CTTTGGCAAG 
AAGGACAAAC AGAAACCGGC CAAGTCGGAG GAGGCTACGT CGCCCACCTC TGGCACAACG 
TCGCCCACGA CGGCCGAGGC AGAGGCTGCT CCAGCTGGGG ATGCAGCTGT CGCAGAACCA 
TCGGTGGCTA CAAATGGCGA GGCTGAAAAG CCAGCTGAGA CTGCCACTGC CACCAGCGAG 
CCGGCGTCCA AGGATGAGAA GCCCGCCGAA AATGGATCAG CGACCGAGCA GGAGAAACAG 
GCCAACGGAG AAACCGAAAA GGCGGCGCCA GCACCTTCCA CAGTTGAGGA AGCGGCGAAG 
CCCAAGCCCG CCGAAGAACC AGCCACAGTC ACTGCCACCG AATCGAACAC CACTGCCACC 
GAAGAAGTTC CCGTGAAAGA GAGCCAGCCA GAGCCCGAAG TGGTGACCAA TGGACATGGA 
GCCGGAGAGG CGCTGACCAA CGGATCAAGC AATGGACTGG CCGAGTCCCC AGTGACCGAG 
ACAGCACCTG TCGCTGACAA CATTCCGAGC AACGTTGATG ACGAGCCACC GCACCAGAAT 
GGCACCAATG GCACCACCAC GCCCCCGCCC ACTCCCGTGG CCACCGAGAT CGAGAAGGGA 
CAGCAAATTG AGGTAAACAA CCACACAAGA ACAGACAACA CCACACAACA CAACAGCAAA 
AACATCACTG TAAATACAAG TACAACAACA ACAAAAACAA CCGAAATCAC AGAGAACACT 
GCACTAAAAA CAATAAGTAC AGATACAGAA AGAACTTGCA CAAGCACTGT GACCGAGACC 
GTTGTAGCAG CCGCAGAGTT AGTTCAGACC CAAACAATCC TAACCAAAAC AACAAACACG 
ATCCTTCCCA CCTCCCACAC GCAGGCCAGC AGTGAAGTGA TTGAAACAGT GACCCCCAGC 
CAAGCGGAAG AGGAAGTTGT GGCAGCAATC ATCAAGGCGG TTAGCAGCGA ACCTGAAGCC 
GAAACGGAGA CTGAGACAGA GGCCGAGGGA TTCGTGCTTG TTGCTCCTGT GTCCACCGAA 
GTCGAAGTTC CCGTTTCCAT TTCCCCCATT GAACCCGTGG CAGAGGTTTC CCAAGTTAAA 
ATTGAACCAC TTGTCGAAAT TCCCGAAGTT GAAGCCAAGT CCGTAGCCGA TGTGTCTGAA 
GCTGATACGG AATCCGTACC AAAAGTTTCT GAATTGAAAA CTGAAGTTTC CGAAATTGAG 
TTCGAGTCCG TTATTGTGGA GACGAGAAGC AGCAGTCCGC CACCTCCATT GCCCAAATCC 
CCGCCTCCGT CTCGTGTCTC CGCATTTGTG CTGTCCGAGG ATGTTATCGA GGAGCAGGTT 
ACCCCGAATG TCCCCGAAGT AAACGACGTG AAGCCCGATG AGATCGAACA GCAGGCAATC 
AGCATAGTGG CTGAAATCAC CGAACAGGCA GCAGAAATTG TCACCGAACA AGAGAAACAA 
CAGGAGGAGG CCAAGGTGGA TTCTGTTCCT GAAACAATTG AGGAATCCAG CTCCACGGTT 
GTGGTTGAGG AAGTTTTACC AGTTCAAAAC GATGAAGTAA CGGCTCCTTC TCCTACTCCT 
GATGATGTCC AGAAACCTAT TGAAGATCAA GATACACCCG ACGAAAAAGA GTCCTACCCA 
GTTCCAGATC CCATTGACCC GGCGGCCGTA AACGATGAGG TCGCTGTTAC AGAAGCTGTA 
GATTGTGAAG TTGAGAAAGA AACCGGTAGC ATCAGCTCCA ACGTTGCTGA ATCTTCTTCA 
GTATCCGACG AGCAGGCGGC AATCGAAAAT CAGGTTGAAA TCCTCGAAGA ACAGACAGTA 
GCTGTTGAGG AAACCACCGA ACAAGAGACA TCAGACCAGC AAGTTATTTC CGAGGAGGCG 
CATAGCGATA ACGATAAGGA GAATGAAATC GACCTGGTTG AAAACATCAT TAGCGATCTG 
GATGCTCCGA TTACCAAAGC AGGCGGCGAT CTGCTGGTAG AATTGGATGC GAGATCCGCG 
GAACAGGAGG GCGAGAGCAA CAACAAGGTG GATCTCGCCA AGGATCTGAA GGAGAAGAAT 
GCCGCCGCGG CAGACGTTAC AACACAGGAA CAACTGCCCG TTACATGCGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Akap200 ( NM_001298824.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285753.1 Drosophila melanogaster A kinase anchor protein 200 (Akap200), transcript variant G, mRNA.

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CloneID	OFa16478
Clone ID Related Accession (Same CDS sequence)	NM_175984.1 , NM_001298824.1
Accession Version	NM_001298824.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	A kinase anchor protein 200, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTAAAG CTCAGAGCAA GCGTTCAATC GACATCACCA CCGATCCCAA GAAGGTCGGA 
GAGGGCGATG AGGTCGCCGG CAAGGTGGAG AAAATCGATG TGGACCAGAA GACGGACGCG 
CCGGCTGTGA ACGGAGACGC TGCCACGCCC AAAGAGGGTG GTGATGAAGC GGCGGCGGTA 
GAGAAAAAGG AAACTGAGGA GCATTCTGAG AACGACAAAG ATCTGACCAC CGAAAAGAGT 
GCCGCAGTCG CTGAGGGTGG TGATGCGGTC GCTGAAACGG CCAAGGGCGA GGAGGGATCT 
CCCAAGGAGG CTGCTGCTGG TGAGGATATA ACCCCGCTGG CAGACGAAAG CATCAAGTCC 
AAGTCCAAGA AGGACAAGGT CAAGAAGAAG TGGTCCTTCC GAAGCATTTC CTTTGGCAAG 
AAGGACAAAC AGAAACCGGC CAAGTCGGAG GAGGCTACGT CGCCCACCTC TGGCACAACG 
TCGCCCACGA CGGCCGAGGC AGAGGCTGCT CCAGCTGGGG ATGCAGCTGT CGCAGAACCA 
TCGGTGGCTA CAAATGGCGA GGCTGAAAAG CCAGCTGAGA CTGCCACTGC CACCAGCGAG 
CCGGCGTCCA AGGATGAGAA GCCCGCCGAA AATGGATCAG CGACCGAGCA GGAGAAACAG 
GCCAACGGAG AAACCGAAAA GGCGGCGCCA GCACCTTCCA CAGTTGAGGA AGCGGCGAAG 
CCCAAGCCCG CCGAAGAACC AGCCACAGTC ACTGCCACCG AATCGAACAC CACTGCCACC 
GAAGAAGTTC CCGTGAAAGA GAGCCAGCCA GAGCCCGAAG TGGTGACCAA TGGACATGGA 
GCCGGAGAGG CGCTGACCAA CGGATCAAGC AATGGACTGG CCGAGTCCCC AGTGACCGAG 
ACAGCACCTG TCGCTGACAA CATTCCGAGC AACGTTGATG ACGAGCCACC GCACCAGAAT 
GGCACCAATG GCACCACCAC GCCCCCGCCC ACTCCCGTGG CCACCGAGAT CGAGAAGGGA 
CAGCAAATTG AGGTAAACAA CCACACAAGA ACAGACAACA CCACACAACA CAACAGCAAA 
AACATCACTG TAAATACAAG TACAACAACA ACAAAAACAA CCGAAATCAC AGAGAACACT 
GCACTAAAAA CAATAAGTAC AGATACAGAA AGAACTTGCA CAAGCACTGT GACCGAGACC 
GTTGTAGCAG CCGCAGAGTT AGTTCAGACC CAAACAATCC TAACCAAAAC AACAAACACG 
ATCCTTCCCA CCTCCCACAC GCAGGCCAGC AGTGAAGTGA TTGAAACAGT GACCCCCAGC 
CAAGCGGAAG AGGAAGTTGT GGCAGCAATC ATCAAGGCGG TTAGCAGCGA ACCTGAAGCC 
GAAACGGAGA CTGAGACAGA GGCCGAGGGA TTCGTGCTTG TTGCTCCTGT GTCCACCGAA 
GTCGAAGTTC CCGTTTCCAT TTCCCCCATT GAACCCGTGG CAGAGGTTTC CCAAGTTAAA 
ATTGAACCAC TTGTCGAAAT TCCCGAAGTT GAAGCCAAGT CCGTAGCCGA TGTGTCTGAA 
GCTGATACGG AATCCGTACC AAAAGTTTCT GAATTGAAAA CTGAAGTTTC CGAAATTGAG 
TTCGAGTCCG TTATTGTGGA GACGAGAAGC AGCAGTCCGC CACCTCCATT GCCCAAATCC 
CCGCCTCCGT CTCGTGTCTC CGCATTTGTG CTGTCCGAGG ATGTTATCGA GGAGCAGGTT 
ACCCCGAATG TCCCCGAAGT AAACGACGTG AAGCCCGATG AGATCGAACA GCAGGCAATC 
AGCATAGTGG CTGAAATCAC CGAACAGGCA GCAGAAATTG TCACCGAACA AGAGAAACAA 
CAGGAGGAGG CCAAGGTGGA TTCTGTTCCT GAAACAATTG AGGAATCCAG CTCCACGGTT 
GTGGTTGAGG AAGTTTTACC AGTTCAAAAC GATGAAGTAA CGGCTCCTTC TCCTACTCCT 
GATGATGTCC AGAAACCTAT TGAAGATCAA GATACACCCG ACGAAAAAGA GTCCTACCCA 
GTTCCAGATC CCATTGACCC GGCGGCCGTA AACGATGAGG TCGCTGTTAC AGAAGCTGTA 
GATTGTGAAG TTGAGAAAGA AACCGGTAGC ATCAGCTCCA ACGTTGCTGA ATCTTCTTCA 
GTATCCGACG AGCAGGCGGC AATCGAAAAT CAGGTTGAAA TCCTCGAAGA ACAGACAGTA 
GCTGTTGAGG AAACCACCGA ACAAGAGACA TCAGACCAGC AAGTTATTTC CGAGGAGGCG 
CATAGCGATA ACGATAAGGA GAATGAAATC GACCTGGTTG AAAACATCAT TAGCGATCTG 
GATGCTCCGA TTACCAAAGC AGGCGGCGAT CTGCTGGTAG AATTGGATGC GAGATCCGCG 
GAACAGGAGG GCGAGAGCAA CAACAAGGTG GATCTCGCCA AGGATCTGAA GGAGAAGAAT 
GCCGCCGCGG CAGACGTTAC AACACAGGAA CAACTGCCCG TTACATGCGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285753.1
CDS	231..2744
Translation	MGKAQSKRSIDITTDPKKVGEGDEVAGKVEKIDVDQKTDAPAVNGDAATPKEGGDEAAAVEKKETEEHSENDKDLTTEKSAAVAEGGDAVAETAKGEEGSPKEAAAGEDITPLADESIKSKSKKDKVKKKWSFRSISFGKKDKQKPAKSEEATSPTSGTTSPTTAEAEAAPAGDAAVAEPSVATNGEAEKPAETATATSEPASKDEKPAENGSATEQEKQANGETEKAAPAPSTVEEAAKPKPAEEPATVTATESNTTATEEVPVKESQPEPEVVTNGHGAGEALTNGSSNGLAESPVTETAPVADNIPSNVDDEPPHQNGTNGTTTPPPTPVATEIEKGQQIEVNNHTRTDNTTQHNSKNITVNTSTTTTKTTEITENTALKTISTDTERTCTSTVTETVVAAAELVQTQTILTKTTNTILPTSHTQASSEVIETVTPSQAEEEVVAAIIKAVSSEPEAETETETEAEGFVLVAPVSTEVEVPVSISPIEPVAEVSQVKIEPLVEIPEVEAKSVADVSEADTESVPKVSELKTEVSEIEFESVIVETRSSSPPPPLPKSPPPSRVSAFVLSEDVIEEQVTPNVPEVNDVKPDEIEQQAISIVAEITEQAAEIVTEQEKQQEEAKVDSVPETIEESSSTVVVEEVLPVQNDEVTAPSPTPDDVQKPIEDQDTPDEKESYPVPDPIDPAAVNDEVAVTEAVDCEVEKETGSISSNVAESSSVSDEQAAIENQVEILEEQTVAVEETTEQETSDQQVISEEAHSDNDKENEIDLVENIISDLDAPITKAGGDLLVELDARSAEQEGESNNKVDLAKDLKEKNAAAADVTTQEQLPVTCE

Target ORF information:

RefSeq Version	NM_001298824.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster A kinase anchor protein 200 (Akap200), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298824.1

ORF Insert Sequence:

ATGGGTAAAG CTCAGAGCAA GCGTTCAATC GACATCACCA CCGATCCCAA GAAGGTCGGA 
GAGGGCGATG AGGTCGCCGG CAAGGTGGAG AAAATCGATG TGGACCAGAA GACGGACGCG 
CCGGCTGTGA ACGGAGACGC TGCCACGCCC AAAGAGGGTG GTGATGAAGC GGCGGCGGTA 
GAGAAAAAGG AAACTGAGGA GCATTCTGAG AACGACAAAG ATCTGACCAC CGAAAAGAGT 
GCCGCAGTCG CTGAGGGTGG TGATGCGGTC GCTGAAACGG CCAAGGGCGA GGAGGGATCT 
CCCAAGGAGG CTGCTGCTGG TGAGGATATA ACCCCGCTGG CAGACGAAAG CATCAAGTCC 
AAGTCCAAGA AGGACAAGGT CAAGAAGAAG TGGTCCTTCC GAAGCATTTC CTTTGGCAAG 
AAGGACAAAC AGAAACCGGC CAAGTCGGAG GAGGCTACGT CGCCCACCTC TGGCACAACG 
TCGCCCACGA CGGCCGAGGC AGAGGCTGCT CCAGCTGGGG ATGCAGCTGT CGCAGAACCA 
TCGGTGGCTA CAAATGGCGA GGCTGAAAAG CCAGCTGAGA CTGCCACTGC CACCAGCGAG 
CCGGCGTCCA AGGATGAGAA GCCCGCCGAA AATGGATCAG CGACCGAGCA GGAGAAACAG 
GCCAACGGAG AAACCGAAAA GGCGGCGCCA GCACCTTCCA CAGTTGAGGA AGCGGCGAAG 
CCCAAGCCCG CCGAAGAACC AGCCACAGTC ACTGCCACCG AATCGAACAC CACTGCCACC 
GAAGAAGTTC CCGTGAAAGA GAGCCAGCCA GAGCCCGAAG TGGTGACCAA TGGACATGGA 
GCCGGAGAGG CGCTGACCAA CGGATCAAGC AATGGACTGG CCGAGTCCCC AGTGACCGAG 
ACAGCACCTG TCGCTGACAA CATTCCGAGC AACGTTGATG ACGAGCCACC GCACCAGAAT 
GGCACCAATG GCACCACCAC GCCCCCGCCC ACTCCCGTGG CCACCGAGAT CGAGAAGGGA 
CAGCAAATTG AGGTAAACAA CCACACAAGA ACAGACAACA CCACACAACA CAACAGCAAA 
AACATCACTG TAAATACAAG TACAACAACA ACAAAAACAA CCGAAATCAC AGAGAACACT 
GCACTAAAAA CAATAAGTAC AGATACAGAA AGAACTTGCA CAAGCACTGT GACCGAGACC 
GTTGTAGCAG CCGCAGAGTT AGTTCAGACC CAAACAATCC TAACCAAAAC AACAAACACG 
ATCCTTCCCA CCTCCCACAC GCAGGCCAGC AGTGAAGTGA TTGAAACAGT GACCCCCAGC 
CAAGCGGAAG AGGAAGTTGT GGCAGCAATC ATCAAGGCGG TTAGCAGCGA ACCTGAAGCC 
GAAACGGAGA CTGAGACAGA GGCCGAGGGA TTCGTGCTTG TTGCTCCTGT GTCCACCGAA 
GTCGAAGTTC CCGTTTCCAT TTCCCCCATT GAACCCGTGG CAGAGGTTTC CCAAGTTAAA 
ATTGAACCAC TTGTCGAAAT TCCCGAAGTT GAAGCCAAGT CCGTAGCCGA TGTGTCTGAA 
GCTGATACGG AATCCGTACC AAAAGTTTCT GAATTGAAAA CTGAAGTTTC CGAAATTGAG 
TTCGAGTCCG TTATTGTGGA GACGAGAAGC AGCAGTCCGC CACCTCCATT GCCCAAATCC 
CCGCCTCCGT CTCGTGTCTC CGCATTTGTG CTGTCCGAGG ATGTTATCGA GGAGCAGGTT 
ACCCCGAATG TCCCCGAAGT AAACGACGTG AAGCCCGATG AGATCGAACA GCAGGCAATC 
AGCATAGTGG CTGAAATCAC CGAACAGGCA GCAGAAATTG TCACCGAACA AGAGAAACAA 
CAGGAGGAGG CCAAGGTGGA TTCTGTTCCT GAAACAATTG AGGAATCCAG CTCCACGGTT 
GTGGTTGAGG AAGTTTTACC AGTTCAAAAC GATGAAGTAA CGGCTCCTTC TCCTACTCCT 
GATGATGTCC AGAAACCTAT TGAAGATCAA GATACACCCG ACGAAAAAGA GTCCTACCCA 
GTTCCAGATC CCATTGACCC GGCGGCCGTA AACGATGAGG TCGCTGTTAC AGAAGCTGTA 
GATTGTGAAG TTGAGAAAGA AACCGGTAGC ATCAGCTCCA ACGTTGCTGA ATCTTCTTCA 
GTATCCGACG AGCAGGCGGC AATCGAAAAT CAGGTTGAAA TCCTCGAAGA ACAGACAGTA 
GCTGTTGAGG AAACCACCGA ACAAGAGACA TCAGACCAGC AAGTTATTTC CGAGGAGGCG 
CATAGCGATA ACGATAAGGA GAATGAAATC GACCTGGTTG AAAACATCAT TAGCGATCTG 
GATGCTCCGA TTACCAAAGC AGGCGGCGAT CTGCTGGTAG AATTGGATGC GAGATCCGCG 
GAACAGGAGG GCGAGAGCAA CAACAAGGTG GATCTCGCCA AGGATCTGAA GGAGAAGAAT 
GCCGCCGCGG CAGACGTTAC AACACAGGAA CAACTGCCCG TTACATGCGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Akap200 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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